LOCUS       DAHUWM010000001        43615 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHUWM010000001 DAHUWM010000000
VERSION     DAHUWM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.001"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..334)
                     /locus_tag="NK788_000001"
     CDS             complement(<1..334)
                     /locus_tag="NK788_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCH6768383.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYP"
     gene            complement(327..761)
                     /locus_tag="NK788_000002"
     CDS             complement(327..761)
                     /locus_tag="NK788_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCH6768384.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            899..1636
                     /locus_tag="NK788_000003"
     CDS             899..1636
                     /locus_tag="NK788_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768385.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1629..2840
                     /locus_tag="NK788_000004"
     CDS             1629..2840
                     /locus_tag="NK788_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6768386.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3044..3826
                     /locus_tag="NK788_000005"
     CDS             3044..3826
                     /locus_tag="NK788_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCH6768387.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            3896..4558
                     /gene="trmB"
                     /locus_tag="NK788_000006"
     CDS             3896..4558
                     /gene="trmB"
                     /locus_tag="NK788_000006"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCH6768388.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4656..6410)
                     /gene="ade"
                     /locus_tag="NK788_000007"
     CDS             complement(4656..6410)
                     /gene="ade"
                     /locus_tag="NK788_000007"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCH6768389.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6542..7471
                     /gene="tadA"
                     /locus_tag="NK788_000008"
     CDS             6542..7471
                     /gene="tadA"
                     /locus_tag="NK788_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCH6768390.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7428..8477
                     /locus_tag="NK788_000009"
     CDS             7428..8477
                     /locus_tag="NK788_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCH6768391.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8474..8752
                     /locus_tag="NK788_000010"
     CDS             8474..8752
                     /locus_tag="NK788_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCH6768392.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8727..9197
                     /locus_tag="NK788_000011"
     CDS             8727..9197
                     /locus_tag="NK788_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCH6768393.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9214..9462
                     /locus_tag="NK788_000012"
     CDS             9214..9462
                     /locus_tag="NK788_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768394.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNM"
     gene            9533..9904
                     /locus_tag="NK788_000013"
     CDS             9533..9904
                     /locus_tag="NK788_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCH6768395.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9876..10235
                     /locus_tag="NK788_000014"
     CDS             9876..10235
                     /locus_tag="NK788_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768396.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10407..11414
                     /locus_tag="NK788_000015"
     CDS             10407..11414
                     /locus_tag="NK788_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6768397.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11439..12623
                     /locus_tag="NK788_000016"
     CDS             11439..12623
                     /locus_tag="NK788_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCH6768398.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13060..14643)
                     /locus_tag="NK788_000017"
     CDS             complement(13060..14643)
                     /locus_tag="NK788_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6768399.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15221..15364)
                     /locus_tag="NK788_000018"
     CDS             complement(15221..15364)
                     /locus_tag="NK788_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCH6768400.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15586..16065)
                     /locus_tag="NK788_000019"
     CDS             complement(15586..16065)
                     /locus_tag="NK788_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6768401.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16138..16422)
                     /locus_tag="NK788_000020"
     CDS             complement(16138..16422)
                     /locus_tag="NK788_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768402.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16500..16694)
                     /gene="ssrS"
                     /locus_tag="NK788_000021"
     ncRNA           complement(16500..16694)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NK788_000021"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16766..18052)
                     /locus_tag="NK788_000022"
     CDS             complement(16766..18052)
                     /locus_tag="NK788_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCH6768403.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18190..18861
                     /locus_tag="NK788_000023"
     CDS             18190..18861
                     /locus_tag="NK788_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCH6768404.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18827..19315
                     /locus_tag="NK788_000024"
     CDS             18827..19315
                     /locus_tag="NK788_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCH6768405.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19328..20275
                     /gene="prmA"
                     /locus_tag="NK788_000025"
     CDS             19328..20275
                     /gene="prmA"
                     /locus_tag="NK788_000025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCH6768406.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20276..21040
                     /locus_tag="NK788_000026"
     CDS             20276..21040
                     /locus_tag="NK788_000026"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCH6768407.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21273..23486
                     /locus_tag="NK788_000027"
     CDS             21273..23486
                     /locus_tag="NK788_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCH6768408.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23497..23943
                     /locus_tag="NK788_000028"
     CDS             23497..23943
                     /locus_tag="NK788_000028"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCH6768409.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24114..25373
                     /locus_tag="NK788_000029"
     CDS             24114..25373
                     /locus_tag="NK788_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCH6768410.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25462..26409)
                     /locus_tag="NK788_000030"
     CDS             complement(25462..26409)
                     /locus_tag="NK788_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6768411.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26803..28095
                     /locus_tag="NK788_000031"
     CDS             26803..28095
                     /locus_tag="NK788_000031"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCH6768412.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28110..29879
                     /gene="aspS"
                     /locus_tag="NK788_000032"
     CDS             28110..29879
                     /gene="aspS"
                     /locus_tag="NK788_000032"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCH6768413.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            29988..30863
                     /locus_tag="NK788_000033"
     CDS             29988..30863
                     /locus_tag="NK788_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6768414.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31129..33012
                     /locus_tag="NK788_000034"
     CDS             31129..33012
                     /locus_tag="NK788_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCH6768415.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33087..33371)
                     /locus_tag="NK788_000035"
     CDS             complement(33087..33371)
                     /locus_tag="NK788_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCH6768416.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33361..34095)
                     /locus_tag="NK788_000036"
     CDS             complement(33361..34095)
                     /locus_tag="NK788_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCH6768417.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34224..34847
                     /locus_tag="NK788_000037"
     CDS             34224..34847
                     /locus_tag="NK788_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCH6768418.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35039..36160
                     /locus_tag="NK788_000038"
     CDS             35039..36160
                     /locus_tag="NK788_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCH6768419.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36167..39295
                     /locus_tag="NK788_000039"
     CDS             36167..39295
                     /locus_tag="NK788_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCH6768420.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39314..42508
                     /locus_tag="NK788_000040"
     CDS             39314..42508
                     /locus_tag="NK788_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCH6768421.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42654..43361)
                     /locus_tag="NK788_000041"
     CDS             complement(42654..43361)
                     /locus_tag="NK788_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCH6768422.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14066 a         7747 c         9220 g        12582 t
ORIGIN      
        1 caggatactc tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac
       61 gtggttcggc ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag
      121 gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc
      181 tgttatttaa atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc
      241 ttgtttcatt cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc
      301 caaataacag tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt
      361 tcttgaatag cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata
      421 gaaaaatcat cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat
      481 tgataggcta gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct
      541 ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga
      601 tcatattcaa aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt
      661 gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg
      721 atttcttgat tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata
      781 aagaataaat acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca
      841 aaataccgtc attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat
      901 gagtttaatt atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat
      961 cagtttcgat gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa
     1021 aagtacgacc atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat
     1081 caatcatcta tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc
     1141 agagactcct tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat
     1201 ggcctacgat atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt
     1261 tcggttatct aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa
     1321 agtgatgatc ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt
     1381 tttaggcttg gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa
     1441 agaaggagcc ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga
     1501 tcgttttgtg gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag
     1561 aaaagaattt cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga
     1621 gcaggcacat gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga
     1681 aatatatgcg ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg
     1741 gtgtgggatt atattattct aattggttga aaaccctgca gccaccgttt gcaattggag
     1801 ggctcataat ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg
     1861 cagaggcagc agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg
     1921 aacgagcttt tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat
     1981 tggccatgcc attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc
     2041 tgatcatatt atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc
     2101 gactagatca acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct
     2161 ttttgtttct atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg
     2221 caagtctata ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa
     2281 tggtgcatgt agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag
     2341 atgttcctga aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc
     2401 atattaaaag agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag
     2461 gttctgattt tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg
     2521 gagtagtcga cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt
     2581 tccaactgtt acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa
     2641 tccataagaa tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg
     2701 cgtcggttat ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc
     2761 cgctagcggc ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt
     2821 tgaagaagat gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag
     2881 agcgttgaca gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa
     2941 tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa
     3001 taataagtac tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt
     3061 agaccaagag tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat
     3121 gcgtgatgag gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc
     3181 aagggaagga attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt
     3241 gacagcacaa gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa
     3301 cgacgtgatt gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac
     3361 ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt
     3421 accggaagaa ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca
     3481 gatcccgtca atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa
     3541 ttggttagta tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga
     3601 ccctgcggtg gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca
     3661 gtggctgatc agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta
     3721 tgcggccatg aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg
     3781 gatgaacgaa gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac
     3841 tctgcggaga tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg
     3901 tgtccgaaat cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag
     3961 tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca
     4021 cattgagatt ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga
     4081 aatcaattat attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt
     4141 gatcgaacaa ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga
     4201 gtatttcgct gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa
     4261 aaaacgtcat gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat
     4321 tcttcgtcca aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc
     4381 tctggcaagt ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca
     4441 aagtcaattt gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca
     4501 acgtatttat cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa
     4561 ctaactttct gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat
     4621 tacagatgta atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt
     4681 tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc
     4741 ttcaattcgg gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt
     4801 ttagctgctc ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc
     4861 gaaaaaggtt cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc
     4921 tgtttgttga caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc
     4981 atatcctcgt cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc
     5041 gctatcgctc cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt
     5101 tgatggtgac gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat
     5161 cgttcgcctt gtcggtccac tttttctacc aaatctttag tcactagact attcgggata
     5221 atttcgatca catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat
     5281 ggttgagcgt ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa
     5341 tggtcttgga gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt
     5401 ggcagcttgg taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca
     5461 cctagtgttt tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt
     5521 aatggatcga ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt
     5581 actaaatcat tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt
     5641 gtgatcgccg gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa
     5701 tacataccaa gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc
     5761 cgaatacctg ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg
     5821 tcaatttttt tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta
     5881 tttgctactg cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa
     5941 aaaggggcta atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt
     6001 acacaagaag gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata
     6061 aactcaagac ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta
     6121 gtaactccat gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg
     6181 atatgcacat ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg
     6241 ctttttcctt cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt
     6301 tccatgattt ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca
     6361 ggcttttttt tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct
     6421 cccaagataa atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa
     6481 attcatttta tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat
     6541 aatggaaata aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca
     6601 agacgtatat atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa
     6661 acgaacattc tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa
     6721 gtatttggga cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta
     6781 ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg
     6841 agagagttta gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag
     6901 ttctcgagat tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag
     6961 cgggacagtc ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt
     7021 gcaagtgatt actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca
     7081 gatcaatccg aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg
     7141 tccagacttg ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag
     7201 agcagcacta acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac
     7261 attggtcaga atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg
     7321 tgtcatttat caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta
     7381 tcaatttatg gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca
     7441 ggaaaatatt tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt
     7501 ttgcacaaac catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt
     7561 tattactcac gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa
     7621 aggaagggca agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg
     7681 tacagatcga gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca
     7741 aacagtttcg gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga
     7801 tgctattgct cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc
     7861 agcttattgg ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct
     7921 ttcattggta tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt
     7981 atcgtctgat gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga
     8041 ttggaccgat atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt
     8101 ttcatgaagg tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa
     8161 gtctgattca gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg
     8221 ctgagcagtt ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg
     8281 gagaggcaaa gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac
     8341 agcttttcaa acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg
     8401 tagcggcttt gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag
     8461 aggaggtttt gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga
     8521 aatgctaatc gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc
     8581 gcgttatagg aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac
     8641 acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt
     8701 gaatgaaggc tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg
     8761 ttttagcatc aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga
     8821 tcctttcttt tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat
     8881 ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga
     8941 tccaaacagg cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc
     9001 cagataaaat aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg
     9061 cttctattac ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt
     9121 attgggaaga aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata
     9181 caaaaagaat tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc
     9241 gcttctgtct gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag
     9301 aaaagtgggg aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg
     9361 atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc
     9421 gataataatg gagtaatcga aaaagtgttt gtcaatatgt agaaagaagc gatcagtatt
     9481 gaaaaaacaa gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt
     9541 gagcagtcta ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt
     9601 tttgcagcgt aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact
     9661 caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa
     9721 acagtatatc atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca
     9781 tcaacctatg ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc
     9841 tttttatgta cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca
     9901 gtagaaaatg ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta
     9961 cgtttttatt gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa
    10021 tcaaaaatta ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc
    10081 ttgccgaaag ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg
    10141 tacaacagtc cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag
    10201 aagtaaaaaa tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct
    10261 tttctgttat aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag
    10321 tgaaaacgat tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta
    10381 taatatatct agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc
    10441 aaaacctgga agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct
    10501 atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc
    10561 cgacaaaaga gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt
    10621 tcgagccaga agaatggcgc agactgtctc aattactttt attgaagggc agccagacag
    10681 aacatttgca ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc
    10741 ttattgaaca actatacaca aataaaaaag caccggttaa gattctggat attgcggcag
    10801 gaatgggcaa cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag
    10861 aaggaattgg tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt
    10921 tgacacaagc aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc
    10981 ctgtcgattt tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa
    11041 aagagttttt aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc
    11101 aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt
    11161 tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag
    11221 gaatgattca attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga
    11281 tcttacagaa aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg
    11341 cttcattaaa agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat
    11401 catctgctct gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca
    11461 atgctgggag ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg
    11521 cgaaagggat cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg
    11581 ataatcaaaa attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc
    11641 tgttagatca gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag
    11701 ggcaccgtgt tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag
    11761 taatggaccg cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa
    11821 tggggatcaa agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata
    11881 catctttcca ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt
    11941 atgaagattt caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg
    12001 aacgtgcggc aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact
    12061 taggaaatgg tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg
    12121 gattcacacc attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg
    12181 ttctcccata tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga
    12241 acaagaaatc tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa
    12301 agaactatga aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa
    12361 aatacatcgg cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg
    12421 gtatcggcga gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg
    12481 gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa
    12541 aagaatcaaa agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg
    12601 atgtggagcg tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg
    12661 caagagggaa ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac
    12721 ttcaatttcc cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca
    12781 aaagggaaaa gactggcatt ttatttattg attttcagga aaatcccaat attttatttt
    12841 cattcatctt tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct
    12901 cgtgggctgt cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt
    12961 cattgatcga ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca
    13021 caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag
    13081 cggctggcgg ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa
    13141 aaagtagcgg tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc
    13201 aatttcttct cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt
    13261 gattttttct aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc
    13321 tttgatccgt ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc
    13381 gacttcttcg tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc
    13441 aaactgcagc tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc
    13501 accgtaaggc ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt
    13561 gtcgccgatg atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag
    13621 tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt
    13681 tccaccatta gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt
    13741 cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc
    13801 ttcatgtgcc cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg
    13861 ctgcagttcg taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg
    13921 gctgtagttt ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt
    13981 gacttgatcg agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg
    14041 taaatagttt tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag
    14101 taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc
    14161 tccgctaagt tctgttaacc gtgcttcctg acagtgtgga atccctaagc cttgcaagat
    14221 cgctgcaatc cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc
    14281 accgtaacgg gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta
    14341 gcgactcatt tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt
    14401 gacggtcgct tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt
    14461 gcgactgata acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga
    14521 tttgccagtt ccgttgatat cgactaaggc aattttttca ccagcttcaa tttgacagga
    14581 taaatcctca aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat
    14641 catgatctct ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt
    14701 taaactcaga ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca
    14761 actgttttgg acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat
    14821 gcgatatggt atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta
    14881 ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac
    14941 tagcgctgac atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat
    15001 ctgactgagg gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca
    15061 ggcgaaatca tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg
    15121 cttaataccg ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag
    15181 gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac
    15241 cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga
    15301 taaaatcggt atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt
    15361 ccacattgac gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg
    15421 actaatcgcc ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt
    15481 tattttaaac attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt
    15541 ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg
    15601 gtctttagct ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc
    15661 gtttctaata acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc
    15721 tgtggctcct aacataatca aatctacttg attatcttct gggatttctc tggcaataat
    15781 ttgttttggt gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc
    15841 attgctttca taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat
    15901 tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag
    15961 ttctccgtta tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc
    16021 agaaccatct acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc
    16081 aagtcaatcg tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta
    16141 ctgctttggt tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat
    16201 tacgatcagt aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc
    16261 cattacgcct aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc
    16321 atttttgttg atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa
    16381 taataatact aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat
    16441 aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg
    16501 taatctccgt taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg
    16561 ggtcccatgg tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca
    16621 agacgggtcc caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa
    16681 catcacagat ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct
    16741 cgcagaaaca gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta
    16801 tacggtaata ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat
    16861 actgagcatt cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat
    16921 gatgaggata ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat
    16981 ctctcaaatg atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat
    17041 ctaatgccat gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg
    17101 ctagtgggat acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg
    17161 tacgagccgc agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac
    17221 gccggcaaat acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag
    17281 catccacatc acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat
    17341 ctccattttt gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg
    17401 taagatgaat ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat
    17461 tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt
    17521 ctaactggat tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg
    17581 ccaactggat gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga
    17641 tcgctggatt gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc
    17701 cactttctaa atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat
    17761 ccaataagag gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct
    17821 tttttgaatc tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg
    17881 cacttgcgat actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca
    17941 tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa
    18001 cgacttcatc gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt
    18061 cctccttctt gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa
    18121 cagccttctg ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag
    18181 gtgagaaaaa tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca
    18241 gaagtggcac gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta
    18301 gctggatata ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc
    18361 tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac
    18421 ctttacacga tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct
    18481 caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat
    18541 cgtggcggaa aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta
    18601 gcaattcctc aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt
    18661 gagaaaaaaa agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa
    18721 ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta
    18781 caaagaaaaa accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga
    18841 tctattaaca tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt
    18901 agattgggat acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa
    18961 agcacaattt gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt
    19021 tgaagacggc gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct
    19081 agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt
    19141 ggaatatcta aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga
    19201 agacgatgac gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt
    19261 agaagtaaaa cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt
    19321 gacagatatg aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt
    19381 tgctaatatt ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt
    19441 tgaacatttt cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct
    19501 gaaagaaggc gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt
    19561 gccttttatc aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa
    19621 taaagtaaca gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta
    19681 tcatccggtg cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac
    19741 agaagatcct ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac
    19801 tcatcccacg acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac
    19861 attgctagat gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc
    19921 aaaagatgtc tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat
    19981 ggatatgaat gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat
    20041 cgagattgaa agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat
    20101 cccagatgct tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga
    20161 agagaagaag caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat
    20221 tttccagcaa aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca
    20281 gcggtatttt ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa
    20341 ctatcaccac attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt
    20401 caatgatcaa atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct
    20461 gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag
    20521 tggctatcct aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc
    20581 gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg
    20641 tatgaaacga caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg
    20701 gcagatcgtg ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga
    20761 tgattatgac gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa
    20821 tgcagccaaa attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc
    20881 agaaggtgga ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg
    20941 tggattagga ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc
    21001 tagctatcaa tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga
    21061 gtatcgacag aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta
    21121 tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa
    21181 aacgtataac tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt
    21241 tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac
    21301 ccggtgtcat aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga
    21361 aagcatgcga atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct
    21421 acattattca tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata
    21481 cagtggctac aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc
    21541 tgagagaaga atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga
    21601 aaataaaata taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag
    21661 caatggctca agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc
    21721 gtacgttgaa gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa
    21781 tctatgcgcc tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata
    21841 cagcacttcg ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga
    21901 agagagaaga acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag
    21961 aagagctggg gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc
    22021 gtaaaatgaa agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag
    22081 tgatcgtaga ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga
    22141 aaccaatgcc cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat
    22201 ctttgcatac taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac
    22261 aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa
    22321 aaaatgaaaa agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac
    22381 tcgaacttca agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata
    22441 tctttagtga taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag
    22501 gatccggacc attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg
    22561 gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca
    22621 ttgagatcat gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg
    22681 ctactagcaa agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa
    22741 atgtgatcaa aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta
    22801 aagatatttt gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag
    22861 aagatgatct ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc
    22921 gtctgactga aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag
    22981 aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg
    23041 ttgtcattca aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc
    23101 ctggtgatga tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag
    23161 attgtccgaa tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat
    23221 gggaagatac atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata
    23281 atcgttcagg cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca
    23341 acagcgttga agcacggacg aataaagata aaatggcgac gatccatttg acggttggta
    23401 tccagaattt atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct
    23461 acagtgtacg ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta
    23521 agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt
    23581 gttttattag gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa
    23641 attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat
    23701 gtgggaggac aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga
    23761 aatcgaccaa gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca
    23821 ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat
    23881 atgtcggttt ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa
    23941 taaagtaatc tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat
    24001 aaactctaac ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga
    24061 aaaaaagtta agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag
    24121 aagtaccagt agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca
    24181 acatgttgga ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca
    24241 ataaagaagc tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga
    24301 atttcaaacg aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg
    24361 gagtacagac tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa
    24421 tgacaccgga aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac
    24481 ttgaagcaca gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat
    24541 ctatttgcga gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat
    24601 tagcagaata tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa
    24661 aaggtgtcga gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc
    24721 gacaaaatcc tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag
    24781 atttagatgg tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc
    24841 gtccaacggt tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac
    24901 cagatttgta tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag
    24961 aaatgagacg ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag
    25021 aagtagatgg ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag
    25081 accatgatga acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga
    25141 aagcaaaaga tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt
    25201 ttcgacaaat gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt
    25261 ccgatcagct agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat
    25321 tcaatcagtt tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca
    25381 taaacttatc gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta
    25441 taaattgata aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc
    25501 ttcattggat aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt
    25561 agctccaggt ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg
    25621 cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat
    25681 ttctctgaaa cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa
    25741 ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata
    25801 gggaacaaaa ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg
    25861 atggatcaaa ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt
    25921 caattgttct aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa
    25981 ttcgtgcaat gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata
    26041 aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag
    26101 aaactcagga gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc
    26161 gatgacgaca cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc
    26221 ggaacggccg tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc
    26281 tgtacgaact acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc
    26341 aacggcattt gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat
    26401 ttctgccata gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa
    26461 ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta
    26521 gtaggttata agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca
    26581 agagaggatc atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga
    26641 gatggacgag tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc
    26701 tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg
    26761 gtgatttttt tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca
    26821 aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca
    26881 gcacgtttat tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac
    26941 tatgaagtga tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat
    27001 gatttttatg acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc
    27061 gttcgttctt ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact
    27121 ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc
    27181 catcaaatcg gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg
    27241 gtgatggcac ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat
    27301 tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta
    27361 ccttttgaat cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc
    27421 gtattggaca gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta
    27481 gattatctaa gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca
    27541 ttggctgttc cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat
    27601 acgatttttg aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca
    27661 ggcggacgtt atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga
    27721 tttgcaatgg gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct
    27781 gcatttaatg agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca
    27841 ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac
    27901 cgtaaagcaa aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg
    27961 atcggtgaag atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa
    28021 gaaaaaagtt ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg
    28081 actttattcg agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag
    28141 tttccgaaca acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag
    28201 accttggggg agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct
    28261 ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg
    28321 tgatcgaagt gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg
    28381 ccactggtga attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc
    28441 cgccatttca aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt
    28501 atttggatct gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa
    28561 aagcgattcg tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag
    28621 ggaaatctac accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac
    28681 atttctatgc gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat
    28741 tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc
    28801 agcctgaatt tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa
    28861 cttacacaga gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat
    28921 tgccatttcc tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg
    28981 atacacgttt tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca
    29041 aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg
    29101 ctgcagatcg ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg
    29161 gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta
    29221 aattcatggg agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact
    29281 tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga
    29341 cacgtttagg aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa
    29401 ctgattggcc acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc
    29461 cattcacgat gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct
    29521 atgcagaagc ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta
    29581 ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa
    29641 tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg
    29701 gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg
    29761 aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta
    29821 tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc
    29881 cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg
    29941 aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat
    30001 tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt
    30061 tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta
    30121 ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac
    30181 cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa
    30241 tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc
    30301 atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg
    30361 ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag
    30421 atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct
    30481 acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt
    30541 acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa
    30601 agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa
    30661 actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata
    30721 agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa
    30781 tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc
    30841 gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag
    30901 acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg
    30961 cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat
    31021 gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat
    31081 acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa
    31141 aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg
    31201 gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt
    31261 ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa
    31321 caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg
    31381 gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg
    31441 ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa
    31501 atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc
    31561 tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg
    31621 atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc
    31681 tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac
    31741 ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca
    31801 agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga
    31861 ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc
    31921 atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca
    31981 gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc
    32041 aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag
    32101 gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta
    32161 atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt
    32221 cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca
    32281 tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg
    32341 aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa
    32401 ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc
    32461 cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg
    32521 gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa
    32581 gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa
    32641 ttaaaggaac aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca
    32701 ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg
    32761 gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag
    32821 atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat
    32881 agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt
    32941 cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac
    33001 gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt
    33061 ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt
    33121 gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt
    33181 gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt
    33241 tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga
    33301 tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt
    33361 tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt
    33421 gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa
    33481 caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat
    33541 tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt
    33601 aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt
    33661 atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag
    33721 ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga
    33781 taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc
    33841 gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc
    33901 ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat
    33961 cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga
    34021 aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc
    34081 gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat
    34141 ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc
    34201 ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac
    34261 tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg
    34321 agaattacat actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag
    34381 ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc
    34441 tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa
    34501 gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc
    34561 ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt
    34621 ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca
    34681 agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa
    34741 cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg
    34801 acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta
    34861 tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg
    34921 agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt
    34981 gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat
    35041 gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat
    35101 tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga
    35161 ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga
    35221 attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat
    35281 atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc
    35341 gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt
    35401 acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga
    35461 caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt
    35521 tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc
    35581 tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac
    35641 accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa
    35701 tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg
    35761 cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct
    35821 gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca
    35881 gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa
    35941 tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg
    36001 tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt
    36061 agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg
    36121 gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa
    36181 attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac
    36241 agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga
    36301 tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat
    36361 gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca
    36421 gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa
    36481 cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga
    36541 aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct
    36601 cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt
    36661 ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt
    36721 ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca
    36781 tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc
    36841 agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa
    36901 acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga
    36961 aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat
    37021 aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat
    37081 tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa
    37141 agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac
    37201 tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa
    37261 aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc
    37321 cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt
    37381 gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca
    37441 agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa
    37501 atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct
    37561 cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct
    37621 attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat
    37681 tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga
    37741 acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga
    37801 acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag
    37861 tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc
    37921 aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt
    37981 tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga
    38041 tagacgaaaa agcaagatgg ccaatgaaag aatagagctg gaagaaataa gacaacaagc
    38101 agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca
    38161 gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag
    38221 taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca
    38281 actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac
    38341 acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca
    38401 agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg
    38461 tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca
    38521 tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga
    38581 gacattccca gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga
    38641 aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta
    38701 cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt
    38761 acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta
    38821 tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt
    38881 aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac
    38941 cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct
    39001 gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat
    39061 ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc
    39121 attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg
    39181 aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat
    39241 aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg
    39301 gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata
    39361 cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc
    39421 agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga
    39481 ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga
    39541 actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga
    39601 accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac
    39661 acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga
    39721 gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt
    39781 ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac
    39841 agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt
    39901 tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt
    39961 attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc
    40021 agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca
    40081 gataaatgaa caaaacgatc aaggaataca attttctgat caaaacaaac ctttatcatt
    40141 caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt
    40201 ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca
    40261 acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt
    40321 gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat
    40381 cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt
    40441 tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg
    40501 tgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt
    40561 gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca
    40621 gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt
    40681 ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg
    40741 agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc
    40801 atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct
    40861 gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt
    40921 tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg
    40981 tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt
    41041 ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca
    41101 tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca
    41161 aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc
    41221 cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga
    41281 agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca
    41341 ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa
    41401 agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt
    41461 aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc
    41521 acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc
    41581 tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt
    41641 gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc
    41701 agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt
    41761 gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa
    41821 aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga
    41881 agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg
    41941 ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt
    42001 aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt
    42061 tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta
    42121 tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg
    42181 cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga atctcacggg
    42241 agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc
    42301 tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga
    42361 aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa
    42421 agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat
    42481 tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag
    42541 aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat
    42601 tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca
    42661 aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt
    42721 ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt
    42781 tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc
    42841 atatgaaatt ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt
    42901 gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt
    42961 ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc
    43021 ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc
    43081 tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct
    43141 tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt
    43201 acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct
    43261 gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca
    43321 tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg
    43381 ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag
    43441 ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat
    43501 atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa
    43561 tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcg
//
LOCUS       DAHUWM010000002        40611 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHUWM010000002 DAHUWM010000000
VERSION     DAHUWM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.002"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            111..1988
                     /gene="tdc"
                     /locus_tag="NK788_000042"
     CDS             111..1988
                     /gene="tdc"
                     /locus_tag="NK788_000042"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCH6768423.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2231..3652
                     /locus_tag="NK788_000043"
     CDS             2231..3652
                     /locus_tag="NK788_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6768424.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3762..5129
                     /gene="nhaC"
                     /locus_tag="NK788_000044"
     CDS             3762..5129
                     /gene="nhaC"
                     /locus_tag="NK788_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCH6768425.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(5410..6429)
                     /locus_tag="NK788_000045"
     CDS             complement(5410..6429)
                     /locus_tag="NK788_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6768426.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(6451..7458)
                     /locus_tag="NK788_000046"
     CDS             complement(6451..7458)
                     /locus_tag="NK788_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCH6768427.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(7451..8116)
                     /gene="pgmB"
                     /locus_tag="NK788_000047"
     CDS             complement(7451..8116)
                     /gene="pgmB"
                     /locus_tag="NK788_000047"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCH6768428.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(8109..10403)
                     /locus_tag="NK788_000048"
     CDS             complement(8109..10403)
                     /locus_tag="NK788_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCH6768429.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10663..12828
                     /locus_tag="NK788_000049"
     CDS             10663..12828
                     /locus_tag="NK788_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6768430.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            13132..13965
                     /locus_tag="NK788_000050"
     CDS             13132..13965
                     /locus_tag="NK788_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCH6768431.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(14067..15767)
                     /locus_tag="NK788_000051"
     CDS             complement(14067..15767)
                     /locus_tag="NK788_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768432.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            16274..17434
                     /locus_tag="NK788_000052"
     CDS             16274..17434
                     /locus_tag="NK788_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6768433.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17450..17893
                     /locus_tag="NK788_000053"
     CDS             17450..17893
                     /locus_tag="NK788_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6768434.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(18376..18906)
                     /locus_tag="NK788_000054"
     CDS             complement(18376..18906)
                     /locus_tag="NK788_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCH6768435.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            19011..19562
                     /locus_tag="NK788_000055"
     CDS             19011..19562
                     /locus_tag="NK788_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6768436.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(19715..20188)
                     /locus_tag="NK788_000056"
     CDS             complement(19715..20188)
                     /locus_tag="NK788_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6768437.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            20326..21798
                     /locus_tag="NK788_000057"
     CDS             20326..21798
                     /locus_tag="NK788_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCH6768438.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            21795..22505
                     /locus_tag="NK788_000058"
     CDS             21795..22505
                     /locus_tag="NK788_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCH6768439.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            22857..23141
                     /locus_tag="NK788_000059"
     CDS             22857..23141
                     /locus_tag="NK788_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCH6768440.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(23422..23841)
                     /locus_tag="NK788_000060"
     CDS             complement(23422..23841)
                     /locus_tag="NK788_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCH6768441.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(23843..24427)
                     /locus_tag="NK788_000061"
     CDS             complement(23843..24427)
                     /locus_tag="NK788_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCH6768442.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(24474..25949)
                     /locus_tag="NK788_000062"
     CDS             complement(24474..25949)
                     /locus_tag="NK788_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCH6768443.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            26130..26441
                     /locus_tag="NK788_000063"
     CDS             26130..26441
                     /locus_tag="NK788_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768444.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(26556..28892)
                     /locus_tag="NK788_000064"
     CDS             complement(26556..28892)
                     /locus_tag="NK788_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6768445.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            29034..29702
                     /locus_tag="NK788_000065"
     CDS             29034..29702
                     /locus_tag="NK788_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6768446.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            29705..30748
                     /locus_tag="NK788_000066"
     CDS             29705..30748
                     /locus_tag="NK788_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6768447.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(30985..31866)
                     /locus_tag="NK788_000067"
     CDS             complement(30985..31866)
                     /locus_tag="NK788_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6768448.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            32025..32753
                     /locus_tag="NK788_000068"
     CDS             32025..32753
                     /locus_tag="NK788_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCH6768449.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32909..33226
                     /locus_tag="NK788_000069"
     CDS             32909..33226
                     /locus_tag="NK788_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6768450.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            33219..33827
                     /locus_tag="NK788_000070"
     CDS             33219..33827
                     /locus_tag="NK788_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCH6768451.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            33882..34673
                     /locus_tag="NK788_000071"
     CDS             33882..34673
                     /locus_tag="NK788_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCH6768452.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            34811..35323
                     /locus_tag="NK788_000072"
     CDS             34811..35323
                     /locus_tag="NK788_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCH6768453.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            35349..36221
                     /locus_tag="NK788_000073"
     CDS             35349..36221
                     /locus_tag="NK788_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCH6768454.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            36397..36759
                     /locus_tag="NK788_000074"
     CDS             36397..36759
                     /locus_tag="NK788_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6768455.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            36768..37520
                     /locus_tag="NK788_000075"
     CDS             36768..37520
                     /locus_tag="NK788_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768456.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            37534..39336
                     /locus_tag="NK788_000076"
     CDS             37534..39336
                     /locus_tag="NK788_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6768457.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            39613..>40611
                     /locus_tag="NK788_000077"
     CDS             39613..>40611
                     /locus_tag="NK788_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCH6768458.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQV"
BASE COUNT        12611 a         7215 c         8411 g        12374 t
ORIGIN      
        1 catattaaaa tatgattaaa aatcaggcca aaacttagtt tacagaatag aagaacaggt
       61 gtaatgtagg gctcgacaaa atttaaatat ttttttagga gggtttcact atgagtgaat
      121 cattgtcgaa agatctaaac ctaaatgccc tattcattgg ggacaaagct gagaacggcc
      181 aaatctataa agctttgttg aatgaattgg tggatgaaca tttaggctgg cgtcaaaact
      241 acatgcctca agacatgcct attattacgc cagaagaaaa aagcagtgct agttttgaac
      301 acactgtgaa taaaacaaag gatgttctat cagaaatttc agcgcggatg cgtacccatt
      361 ccgttccttg gcataatgcc ggacgttact ggggtcatat gaactctgaa acattgatgc
      421 catctttact agcgtacaat tttgctatgc tatggaatgg taataacgtt gcctatgaat
      481 cttctcctgc aacaagccaa atggaagaag aagtaggaat ggaatttgca aaattaatga
      541 gctataaaga tggctggggc cacatcgttg ctgatggttc tttagcaaac ttagaaggac
      601 tttggtatgc ccgcaacatc aaatcattgc cgcttgcaat gaaagaagtg acacctgaac
      661 tagttgctgg aaaaagcgat tgggaattaa tgaacttgtc aacagaagaa atcatgaatc
      721 tgttagacag cgtcccagaa aaaatcgacg aaatcaaagc tcactcagca cgtagcggaa
      781 aacatttaga aaaattagga aaatggttgg ttccacaaac taaacactat tcatggctaa
      841 aagctgccga catcatcggt atcggtttag accaagtgat tccagtgcca gttgaccaca
      901 actatcgcat ggacatcaac gaacttgaaa aaatcgttcg tggtctagca gctgaaaaaa
      961 caccaatcct aggtgtagtc ggtgttgtag gatcaacaga agaaggagca atcgacggta
     1021 tcgataaaat cgttgcattg cgtcgcgtat tagaaaaaga cggtatttac ttctacttac
     1081 acgtagatgc tgcttatggc ggatacggcc gtgcgatttt cttagacgaa gacaacaact
     1141 tcattccatt tgaagattta aaagatgttc attacaaata caatgtcttt acagaaaaca
     1201 aagattatat cttagaagaa gtacacagtg catataaagc aatcgaagaa gcagaatctg
     1261 taacgattga cccacataaa atgggctatg ttccatattc tgccggtggt atcgtcatca
     1321 aagatatccg catgagagac gttatctctt actttgcgac atatgtattt gaaaaaggcg
     1381 cagatatccc agcattactt ggggcttata tcttggaagg ttcaaaagca ggggcaacag
     1441 cagccagcgt ttgggcagca caccatgtct tacctttgaa tgtcacagga tatggtaaat
     1501 taatgggtgc atcaatcgaa ggtgcacatc gtttctacaa cttcttgaat gacttgtcat
     1561 ttaaagtagg agacaaagaa atcgaagttc atccattaac ttatccagat ttcaatatgg
     1621 tagactatgt attcaaagaa aaaggcaacg atgacttagt tgcaatgaat aaattgaacc
     1681 atgacgtata tgattattct tcatacgtga aaggaagcat ttacggtaat gagttcttaa
     1741 cttcacatac tgactttgct atcccagact atggcaacag cccattacaa tttgtgaacc
     1801 aactaggatt ctcagatgaa gaatggaacc gtgccggaaa agtaactgta ttgcgtgcca
     1861 gcgtaatgac accatacatg aacaaagaag aacactttga agaatatgct gaaaaaatca
     1921 aagcagctct tcaagaaaaa ttggaaaaaa tctacgcaga ccaattattg gcaagcgaag
     1981 caaaataagc aaggtaatca tgttagaaaa cagcttgaat actaactgtt tctgacgcat
     2041 gtttggatct gaagagcaag gggagaccta agctcttcga tccctttgtt ttgaggttgt
     2101 ctttgaagaa acccttcaag caatagactc aaagaatccc ctcaaatatt cgctgaattg
     2161 tttcgtttat tgctgaaact acttcaaaga caccgttatt ttatccaaaa aatcaaggag
     2221 agtgtcaacg gtgagtaaat ctgaagtaag taaaatgggt cttgggacct ttatcggtct
     2281 gacaatggca ctgtgtgcaa cggttcggag tattcctacg ttagccgcag tcggttggac
     2341 attgattttt tattcaattt ttgcagtttt gttttttgcc ggtccgatat caatggtttc
     2401 cggtgagctt tccacgatgc ttcctcaaga aggcggtcct cagttatggg taaaaacagc
     2461 actgggcagc aaatggggat ttgtcgttgc atggctatta tgggtgcaaa tgttcccagg
     2521 tatggtaatg gtcgcttcta ctttaggacc attgttagga aacacatttg gtaatgtaga
     2581 acttggcaat aatcactggt tcgttttagg ttgtatttta gttatctact ggatcatcac
     2641 gatcttgaac ttgaaattcg atatggcaaa agtcggcggg aacatcggtg tatggctagg
     2701 ggtatatatc ccagtagtca tcatgttcgt tttaggtgtt ttggcagcct tcaaagtcgg
     2761 actagtctca aacggctatc taggtgactt ttcatggtca aaagctttcc ctgacttgga
     2821 acatattgat tcattgaaat acttagctgg tatcacattt atttttgttg ggatcgaaat
     2881 gagttctgtt tacatgcctc gtttgaaaga cgcaacaaaa aattatacaa aaggcgtatt
     2941 tatcgctttg atcggattag ttttattgaa tgtcatcaat gcgatgcttg tagcaaatgt
     3001 cgtaccagat ggaaaaatgg aattagcaaa tatcacgcag cctatcttga ttgactgtca
     3061 aatcctaggc ttaccagaag tgatcggaaa tatcttcagc ttcatggtat ttattggtgt
     3121 gcttttacaa ttatcagctt gggttactgg accatcaaaa acaatcatcc aagtagctcg
     3181 tgaaggattc cttccaccaa aattcggatt ccataaagaa aacaaatacg gcgtatctag
     3241 aaatgtcgtt ctaacacaat caatcgttat ttcattattt gctttgcttt atggtgtaat
     3301 ggatgacgta agtgctgtat tcttgacact tacaaatgct acaactgtta tttattgtat
     3361 cgtgtacatt ttgattgctg tttcattgct taaaatgcgt aagaaacatc cagaattcga
     3421 acgtccttac cgaattggta aaaacggaaa cggattagct tgggtagtca gctgcatgct
     3481 gatcttctct atcatcgttg tcgtatttgc tacattaggt acagcgacat tgagtgatgc
     3541 attattagta gcagctatca cagtggtcat gttcgttatt ccattgatca tcaatcactt
     3601 caagaaagat tcttggggga ttgaggtaga aaaaagttta gaagaaaaat aaaaaaaata
     3661 agtctttcag ccgacgtttt gtcgctgatt tagtaggagg tcctgccaag tgaatagttt
     3721 tttcatggca gggccttttc atttgaaata ggagatcgac gatgaataaa aatatttctt
     3781 taaaagaaag tatcgctgta ttgatcgtgt tgttggcact cctaggagta ctgatcatcg
     3841 gattccagct ctcgccacat atcccgatcc ttgcagcttt catgtgtttg ttgttttatg
     3901 gcagatttaa aaagttcaca tgggatgaga tccatgacgg gatcatcaaa ggaatcactc
     3961 cagggatcat tccaatcctg atttttcttt tgattggtgt cttagttgct tcatggattt
     4021 tatctggaac catccctacg attatggttt atggatttaa acttgtttct gtgcgtttct
     4081 ttttaccgac agcatttctt gtttgtgcaa ttgtcggagt gactgtcggc agttcattta
     4141 caacgatttc gacgattgga attgcttttt tggggatcgg tcatttactt ggattcaatg
     4201 atgcaatgac tacaggcgca gtcgtttccg gtgccttttt agggaataac tgctcgcctt
     4261 tatcagatac gaccaacttg gcagcgggta ttggaggagt taatctattc gaacatatcg
     4321 ctggtatgcg ttatacggat ttcccagcat tcgtcctttc gttgattttt tatacgatat
     4381 taggacataa cagtcattca gccgatctag ctagtatcaa tgaaatgatc caaaacatga
     4441 aagcgagctt ttggatctct ccttggacat tgattccact agttatttta tttggggggg
     4501 caatcaaaaa aattccggct atccctacat tactagcggg atcgacagct gctctcgttc
     4561 tttcttttat ccatgagaaa agtttgacgg tcggaaaagc agcagacatc ttgatgaatg
     4621 gttatgtcgc aaatacacct gatccaaagc tagatgaact gctttctcga ggcgggatca
     4681 tgagtatgtt aggttctgct agtttgattt tacttgcatt ggcattaggt gggttactga
     4741 tcaaatatct gatcgttgat accatcgttc aagaattaaa agaaaagatg gaccgacctt
     4801 ctcgtttagt cgggtttact gccttgagct gtatcggaat caatctgatc gttggagaac
     4861 agtatctgtc catcattctt ccaggagaaa cattcaagcg ttcttttgat gacgttggct
     4921 tatcaaaaac ctatttgaca cgaacactag cggattcagg tgcgaccgtc aattctctcg
     4981 taccgtgggg agtcagcggc acattcatta tgggaacgat gaaagttagc gcgttgcatt
     5041 acttgccatt cgtctttttc tcgattcttg cacctgtctt tacaatatta ggcggttttt
     5101 tattgaatcg tcgacgtgta aaaaattgaa ggaaggaaaa aagaggctgg gacagaagcg
     5161 tttaactcca agaaataaga agaaactcaa gaaaattgct ttttaaattt ttgtgaattt
     5221 cagcttattt ccgaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag
     5281 acaaaagtgt tctttacttt tgtcccacgc tcttttttac agataaacaa tgtgttagaa
     5341 gtagtatctt cttttacata aaaagctgac cataggataa atggtcagcg tatttagtga
     5401 gtcagctatt tagtatgggt tttctaaagg aacgactgtc tctcttttga tcaagcgatg
     5461 agggatgacc aaacgtacgc cactgctcaa ttcttcatgg atcaattcgc gcagcttttg
     5521 catagcgaca cgtcctaatt cggagatatc gatatcgata cttgtaaggt aagggtgtgt
     5581 taatgttgag aagatcgagt tgttgaagct aatgacggag agtgtctctg gaatgacata
     5641 gccgtacatt tgcgccaact gcatcgtgcg aagggcgaag atatcgtcaa tgaccaccaa
     5701 tgctgtggct tcgttttctt tcaacgtttt ctcaaaggac agataatcct ccggatgctc
     5761 tagtactaca ctggataacg caggaagacc agccatcatc attgcttttt gataaccgaa
     5821 atatcgttcg aaatacaaac tttcatgagt cgtgtttgtc acaaacagga tattcttatg
     5881 tccattctgg atcaaaaatt ctgttgcttg tttacctaga agctggttat cattatctac
     5941 ataaacgatt tcgttctcat gatgataagg ctgtccgatc aaagtgaaag ggatgtggtt
     6001 ttggtataaa taatcaatga caggatcttg actatctgaa tacactagaa taaaaccatc
     6061 tacttgtttt tgtaaatgca tacgctggac gttctctaac agcaattcaa aatttttagc
     6121 agtagcgatt gctgtagtca tatcataatt ccgcgcttct tcgttgattg cttccatgat
     6181 ctcaagataa aatgggttac cgatccgctc ttttgaatca agagggggta agattacgcc
     6241 gatggcactg gaaatgcgtt ttcctagatt gcgggcagtc atgttaggaa cataacctag
     6301 ttcatccata atttttcgga ctttcttctt tgttgtctcg gaaatactag gatgatcgtt
     6361 gatgacacga gagacggtcg aggtggcaac accggctttt ttcgccacat cttttacggt
     6421 aatagccatg ataaaaacac ctttctcttg ttattgtgca gagaaccgat aaatcgtctg
     6481 actgatccgt tcttgaccag gatggaggtt gatcgatcca aatttgggga aatgaacgat
     6541 atcagggatt tcttgcggtt cgattgctaa tccgtagttt gaatgcatct gttttccatt
     6601 gacagagaaa tcagcttcga atccagtcgt agaaaataat accatacttt tattagaagt
     6661 tgctatagtc atttggcgac cagaatgctt ctcgaataat tgtaaggaag ttttggaaag
     6721 cctaggatga tgcaaaagaa atacatcatc tagtccatct ggataacatg caaggatctc
     6781 ttgcagtgtt ggggattttc taaagtcgat tggcagtttt tctttttttg tccaatttcc
     6841 agtaggcagt ttttcttgat ctaattcaag cacaccatct aaaaagaccg ttaactgatg
     6901 ttcatagatg tctcttgttc catttcctga aaggttgaag taggcatggt tggtcggatt
     6961 gcagatggtt tcaacaaggc tactagcaga ggttgtcatc tctaattcgt tttttgttaa
     7021 acggtaagtg atcgtagcag tgattggtcc tggataggta gaaaattcat cttttaagta
     7081 aaagaccaca cctattgatg atggagtttt gaaaacttct acttcccaaa attggaaaga
     7141 ccagcctttc gtgccaccgt ggatatggtg tgatccgcag tttttctcca atctatgctc
     7201 accccaagaa gcatcccgga tccttccggc tacaggtccg acagttgctc caaaaaagct
     7261 tttgtctgtt agaacatctt cgaaacgatc gtaagacaat aaaacatttt ctgattttcc
     7321 ttctttgtct ggtgcgaaga tttggtgcag gcgggcaccg taattgagaa gaacgacttc
     7381 taagtagtcg ttcttcaatg tgattttctt tatttcagtc tctggaactg gtacgacgga
     7441 gatctctgga ttatccatgg tcatgacact tttctttcaa gtaatcgatc gtaagaaaag
     7501 tcgtatcagg gacaagcggg atctcctctc caaggtcttc agcacgtccc acgccaattg
     7561 gttgagcgcc acttgagagg atggcttgga taccggcttg cgcgtcttcg atcccgatac
     7621 attcttctgc ttttagtccg acagcttttg ctgcaaggag gaagatatct ggcgcaggtt
     7681 ttccgtttgt tgcttctgcc gggtcggcaa tagcatcaaa gtaaggtgta agacccattt
     7741 tttcaagtag gaatggcccg tttttacttg cagaagcaag agcaatcttg attttttctt
     7801 cttttaaaga atccagtaaa ggcacgaccc cggggaagac atcttttggt tcgatcgtct
     7861 ggatcatctc taaataataa tcgttttttc ttttcgctaa ttctgcaaat tcttcctttg
     7921 aaaaagagtg ttcttttccg ccataagcta aaagcaaaga gagtgagtct tctcggctga
     7981 cgccttttag ctgttcgttg aatgtgtggt cgatcgtaat gccgatttcc tgaccaagct
     8041 ttcgccaagc acgataatgg aattcagcag tatcggtaat gacaccgtct aaatcaaaca
     8101 ataccccttt aaacatttgc ttcctccttt cttagcgtta tttctagacg atccgctaaa
     8161 gaataagact gttcatagac gtaaatttct agaggaaaac cttctaataa ctcgaaagta
     8221 acttgttcat ctgtgactga gatgaacaat aaacgtccgc gataattgat atggaaagaa
     8281 taagctttcc aagtttttgg cagaaatggt gcgaatctca actgatcatg ggcagttttc
     8341 atttgagcaa atccttggac aatcgacaac cagctaccag tcatggaagt gatgtgcaag
     8401 ccgtcttctg tgtcgttatt gtaattatcc aaatccaagc gagctgttcg ctggtacatt
     8461 tctacagctt tgctttccat tcctaattct gctgctaaaa tcgaatggat acttggcgaa
     8521 agggaagact catggacagt cattggttca tagaaatcaa agtttttttg cttttcttct
     8581 tttgtaaaag tatcattgaa gaaataaatc ccctgtagta catctgcctg cttgatgaag
     8641 caagatctaa ggattttgtc ccaagaccaa tgctgattca gaggaagatc gcttgtggga
     8701 atatcggata caggcatgag atctttatct aagaatgtgt catgctggac aaagattcct
     8761 aattcttcgt caactgggaa gtacatgttc tctgcgatat ctttccattt ggctagttct
     8821 tcttcgctga tagtgatagc agcttctttt tgataagtta aataattttc tcgcgtgtaa
     8881 cgcaagaccc aagctgcgat cgtatttgta taccaattgt tattgatatt gttctcatat
     8941 tcgtttgggc cggtcacacc gtggatcatg tatttatttg ctcgttttga aaaatggaca
     9001 cggtctgccc agaagcgtgc gatttctgct aaaacttcca gtccttcatt tttcaaatat
     9061 tccgtatcgc cggtgtaatt cgtataattg tagattgcat aggcaatcgc gccatttcgg
     9121 tgaatctcct cgaatgtgat ttcccactcg ttatggcatt cgacaccagt gaaggtgacc
     9181 ataggataaa gcgcgccttt taatccttgt tgctgagcgt tgtggattgc ttgcggcagt
     9241 tgattatgcc gatatttcag cagattctta gttacttcag gtttggctaa agcaagataa
     9301 agagggacag catacgcttc tgtatcccag taagtggctc cgccgtattt ttctcctgtg
     9361 aatcctttgg ggccgatatt caatcgttca tcttcgccgt agtaagtaga gaatagttgg
     9421 aacaaattga atcgaatacc ttgttgtgct tcttcgtctc cttcgatgac cacatcggca
     9481 agcttccatc gttctgccca agccgctgtt tgctcttctt ttgcttggat ataattgttt
     9541 gtctggttcg ttaattctga gacagctttt tctgcttgct gcgcttctgg aatatctcta
     9601 ctagtaagaa cgataatttc tttttccact gaccatgtct caccttgaga taattctgca
     9661 gacaagcaac catgtaatgc taaagtatct tcgtgatagt cagggatgac tgattttcca
     9721 ttaacgaaat ggcgcattgc tgcagtaaca gtaaattgtt caatcccaaa gttgtttgga
     9781 atagttttag tcgtcaaata gccgatttct tctatagttc cacgatcaat ctcattccaa
     9841 aaatgttcat catagttgct gtcttcgttt tgaacatttc catccaattt tgatacgagt
     9901 ttgactgttc cgctaccttc aagcatttct atagttaaat agatataagc tgattctttt
     9961 ttgacaatgc tcagaaaacg ttcgaaggaa atgcgtactt tattagtaga agtagtgatt
    10021 gtgaatgtac gagataatac accatttttc atatctaatt cccagtaaaa gtcttctggt
    10081 tccaaagaag ccaagtcgat cagctgatcg ttgatcgaaa tatctatcgc gataaagttc
    10141 aaggcgttga tcacttttcc aaagtattct ggataaccat ttttccacca gccgacacgt
    10201 gtcttatcgg gataccagac acccgccaaa taagtacctt gatggtggtc ccctgagtaa
    10261 gtttcttcaa agttcccgcg catccccata taaccgtttc caatacttgt taaagattct
    10321 tgcagtcgaa gatttttttt atctaattgt gtcgtgctga ttttccatgg atcaatagca
    10381 aatagtcgtt tgatctgttt catttttctc ctcctaatga gctgttttat acgttcataa
    10441 taaacgcaac cgattgcaca agtcaataga caaaagaaaa tatttcatag aaaaaagctt
    10501 aaaaaaataa atttttagga gaaaattata gaagggaaca cacgtattat aggttttata
    10561 gaatggtata gacagaaaaa taaaaaataa agcatttaca aaaaagaaaa cgcacgctat
    10621 aatacgttta acgaaaccga ttgcgtaaag gagctaaacg gaatgaaaaa gatatttagt
    10681 tttgagtttt ggcaaaaatt tggaaaggct ttaatggttg tggtagcagt tatgccggca
    10741 gctgggttga tgatcagtat agggaaatca attcccttga tcaatcctga ttggacgccg
    10801 cttgtcacaa cgggcggagt aattgaaaat attggttggg caattattgg taatcttcat
    10861 attttatttg ctttagcaat aggaggaagt tgggcaaaag aaagagcagg cggagcattt
    10921 gcagctggga tctcatttat tttaatcaat cggatcactg gggctatctt tggtgtcaca
    10981 agtgacatgc ttgcgaatga agatgcattt acccacactc tgttcggtac gaagatcatg
    11041 gttaaaggat tttttacaag tgtattggaa gcaccagctt tgaatatggg agtattcgta
    11101 gggatcatcg ctggatttgt cggggcaatg gcttataata agtactataa ttatagaaaa
    11161 ttgccggatg ctttgtcatt tttcaatgga aagcgctttg ttccgtttgt tgttatctta
    11221 tggtcaacaa tcgtggcatt gattcttgct gtggtgtggc caaacgtaca ggctggaatc
    11281 aataattttg gtttatggat tgcagaatca caagaaagcg caccgattct agcaccgttt
    11341 ttatttggaa cattagaacg tttactattg ccatttggat tgcatcatat gctgacgatt
    11401 cctatcaact atacacagct tggcggatcg tatgagattt tatcaggcgc acaggcgggg
    11461 acacaagtat ttgggcaaga tccgttatgg ttggcttggg ccactgattt agtgaatctt
    11521 aaaggtgcag gggatatgtc gcaatatgag tttgtgttgc ataactggac gccggctcgt
    11581 ttcaaagtag gacaaatgat tggttcttca ggtatcttga tgggaatggc ttttgcgatg
    11641 tatcgtaatg tcgatcctga taagaaagca agatataaat caatgtattt ttcagcagcg
    11701 ttggctgtct ttctaacagg tgtaacagaa ccattggagt tcatgtttat gtttgctgct
    11761 gtgccattat atgtcattta ttcagtgatc caaggggctg catttgcgat ggcagatatc
    11821 ttgccattgc gggtccattc atttgggaat atcgaattgc tgactcggac accgttagct
    11881 atcaaagcgg gtctaggtgg agatcttatc aacttcgtgt tgtgtgtgat cgcttttggt
    11941 gttgttacgt atttcttagc aaatttcttg atcaaaaaat tcaactttgc tacaccggga
    12001 cgtaacggaa attacgacaa tgacagtgaa gaaaccgttg tatctaacag cggaacagga
    12061 acagcagatc agcaagtggt ccagatcatt catttgctgg gaggaaaaga aaatatcaaa
    12121 gatgtcgatg cttgtatgac tcggttacgc gtaagtgtaa atgatcgaga aaaagtaggg
    12181 actgaagaag cctggaaacg agcaggggcg atgggattga tcgtcaaaga caatggagtc
    12241 caagcagttt atggaccaaa agcagatgtc ttgaaatcag atatcgaaga tctgctgcaa
    12301 tcaggagcag agattccaga gccagaaatg gtttctacag gaaatggaca agcaggtagt
    12361 aagcaatact taggtataga acaagaacta gtttccgcag cttctggaga agtgattcct
    12421 ttatctgaag taaaagatcc ggtattctca caaaaaatga tgggtgacgg ctttgctgtt
    12481 attccaacat caagagaagt tgttgcgccg atcgctggaa aagtgacaag tattttccct
    12541 agcaaacatg caattgggct ggaaacgaaa gatggtatcg aagttttgat ccatatggga
    12601 atcgatacag tccaaatgaa tcagccagct tttgagattg ctgtcaaaga aggccaagaa
    12661 gtaggaacag gaacgaaatt agctgagatg gatcttgatg tgatccaaaa cgaagaaaaa
    12721 gatccaacga tcatgatcgt atttacaaac gataaagtag aagaagtagt gatcaagcaa
    12781 ttaggtacag tgacagctgg taaagtgatc ggctccatta aaatgtaagg agaggttgtg
    12841 aaaaagctgt cctgcatcaa gtcataagaa cagccaaaca aaaatagctc ctcctatttt
    12901 tcgtttggtt gggcttatta ccgagaagca gcagtttgaa tactgtttag tggagaggtc
    12961 gtgaaaaagc tgccctgcac caagtgataa aaacagccaa acaaaaaata gctcctggtc
    13021 cattgagctt attactgagg agttagcagg agaacactat ttgctggaga ggttgtgaca
    13081 aaaattttgt cacaaccctt ttgtttcgaa atgaagacaa ggagaaaacc aatgaaattc
    13141 atgacactca acacgcatag ctggcttgag ccagaaccag agaaaaaatt gcaagaatta
    13201 gcagataaga tcctgttaga agattatgaa gtgattgctt tacaagaaat caaccaatta
    13261 ctggaatctg aagaagtaga acctgcgaag ctaatgaagt tctgtcaggt aaagaatcaa
    13321 gtacctatcc gtaaagataa ttttgcctac cgtttagttc agcttttaaa ggaacacgga
    13381 aaagagtatt tttggagttg ggaaatgagc cacatcggtt atgataaata tgaagaagga
    13441 aatgcactgc ttacgaaaaa aacattggaa agtcaggtcc ttaccgtctc ccaatctcaa
    13501 aagaaagaag attatcagac acgcaagatc ttgattggga aaacaaagat agatgatcag
    13561 gacatttttg tctcgagctg ccacttttct tggtggacag ataaaaaaag tggcttttat
    13621 tttgagtgga aaaacttgga aaactacttt ttggaaactc gcgtaccact ttttttccta
    13681 ggtgatttca ataatccagt agactctcaa ggttattata ctgtaagaga aagttgcctt
    13741 cttcttcaag attcgtatgt tgtcgcgaat gaaaaaggaa aagcagctac tgtggaaaag
    13801 aaaatagatg gttgggaaca aaatacagaa aaattgcgta tagattttat tttcgtccct
    13861 gaaggaatgc aggttaaaaa atatcaacgg atttttgatg ggatcgatag tccaatcatc
    13921 agtgatcact acggggtgga aatcgaatta gatgtgaacg aataaaaaat ataacatata
    13981 acataaaaaa actgattaaa ataaaactct ccaaacgatt ataccgtttg gagagttttt
    14041 tgatcggatg tatttatccc ttttgcctaa gcacgtaaaa gcttgtatct atgataaata
    14101 aataaacata gcaaggtaaa ccagccaatc aaagtaagag aaagaatgaa atagaaatag
    14161 attggcgttt gataatgaag cgtgagctcg ttgattcctt ttttctgctt cacgattggg
    14221 gtcccgatat ctgttacttc ataatcagtc aatgtttttc cattttgctg tagcacagtc
    14281 cgatcgtaga cgataacagg gatatgaaca gttttgtccc cagtgttttt ccacttgatt
    14341 gtcaaaagac cgcctggatg tgtttttata aaagttgttt cgttttctag tatttcttcg
    14401 ttataccggt cgtatttatt gtcttttgtc tccttataga ttggcagata gtcaggtgtg
    14461 gttttttgga aaacgaacaa gctggtagac atatctttgc tgaagaatgt tttccttgct
    14521 tcttccggcg tatcgaatag ataagtatgt cgactgacaa atgtttcatt ttcccacttt
    14581 tccagatgct ggtataagtt ctggatcgtc tgtgctgaaa aaataagaat gccaaatata
    14641 cttaaaagac ggaaccactt ttgttcagac cactggttca gtgaaagggc aaggtaaagt
    14701 agaagcaata gcgtaaatgg cacgaaaaat cgaaatggaa actggatcaa actaataaaa
    14761 ggcacatttt tttcgatcaa tgtcgtccat ggaatcaagc tagtagaaag aaaaaagaaa
    14821 acaagacttg tgtacaacac gattttttgg aataaggtgt tcttttgttg aaagaaaata
    14881 aaaagcaaaa aacctgataa tatcaatata ggaaaggcct tagggtaatg gagccaataa
    14941 ctgccatcca gatctatcgc cttttgactc atttctctgt taacaaaggg aggaagaatc
    15001 ttattcccac tgtaaacaat agctaatcct gcccaaatat ttgctgtcag aacaaaataa
    15061 agcaagaccg acttaatcag ttgaagcagt gcttttcctt tttcagtctg gttaaaaaag
    15121 taataaagat aaaaagggat atacatcaaa gctaacatga tcgaagacag taaatggatc
    15181 tgtgtcataa gtcccactga gacagcgact ttgataacag ggaacttttt atgaaggacg
    15241 aaatcagcaa tagggatcat acataaagga taaaaacaag tcccccagct agtgaatcct
    15301 tggttgatag accagtattg aatagaatac gtactcatga ataaaacggc tactggtaca
    15361 cttacccaat ttcgaacatt gacatatctc agtaaaatca gcatagaaaa tcctgaaata
    15421 agataaagaa tgaaatttga aagaatctga taatgaaacc agctacctgc aagcaacacg
    15481 atcaatcctt gtaaataagc gaaaaatggg ccgtaaacgg cattcacgat cctgccactt
    15541 tgctggaatc cataaaggga aggaaaataa tggaaatccc catatttaat ttgttgcgcg
    15601 gcatcataga accgattgta atggaatggg gcatcagcac ccaaaattag tttatgtgtg
    15661 aataaatgag gcatgagaaa tgccaaagca atgaaaaaaa ttgctaaaaa tgggccatat
    15721 tttttcaatt gtcgaactcc tttgcaaact ttagataaat tcatcatata tcctatgcga
    15781 gaggtaggca agaaattaga aaattcatga aaatatttat gaattttaca agaaaaacat
    15841 aagtgaaagt tctaaggaga gaggatgcga cataagtttt gttgcatctc cttgttttga
    15901 aacagaattt ttcttaatac tcgaaacaaa gtagcttttt catgacctct cggtaaacgg
    15961 tgttcaatca gctgcttctc ggtaataagc tcactagcct agaaaatatg agaagctgtt
    16021 ttctgactag cgctcttatt actcaaagca taacgcagtt ttcacaacct cttccttaaa
    16081 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc caacaaaaaa tatgagtagc
    16141 tatttttttg ggctgtactt attacttgat gcaggacagc tttttcacaa cctcatcggc
    16201 tttcattttc tatttttttc ctgaacccat ataataaaag aaagaacaaa aaaacttgca
    16261 agggaggaca catgtgaagc agagtaagca aaaacagtct cgtcgacttt tgtttcgttt
    16321 gtttttatta atatgtatcg tcgtagggtt actgatcgct ttatatccat tttatgtgga
    16381 ttctttaaat agttttattg atcaaaaacg tatcgaacaa gtacaaaaac ggaccgcagc
    16441 tgaaattgaa gtgcagcgaa agaaaatgga ggagaaaaaa caacgattaa ctgaccaagg
    16501 attcaatcct ggtactgatc ctttcaatga aaagaacaga aatgaaagta caacatccag
    16561 ccaattagag gaatggctga tcggaagtat caatatacct aagatccaat taaatatttc
    16621 actttatgat cggctcaatg gtaggatcct tgaaaatgga gcaggcgtct tgcaaggaac
    16681 ctctttcccg cttggtggaa attcgacgca ttcagtgatc tctgctcaca gcggtttacc
    16741 taatcgacgg ttatttaccg aattagatcg tttagaaaac ggagacacgt ttattttgac
    16801 tgttttgggg gagaaattgg cttatcaagt taaaaatata caagtggtac tgccagatga
    16861 cacgtctgtt ctaacgattt cagaaggaga agacttagtt accttgctta catgtacgcc
    16921 gtatatgatc aacacgcatc gtttgctagt gacaggacat cggattcctt ataacgaatc
    16981 ggtaaaaaaa gaagaaggaa aaggaaatca ggaaagaacc atgcggcaat ggtttattct
    17041 tgcaggaacg ataatagctg tagtcatact gttagtgttt atcggacgac taatctatca
    17101 gtatagatta agtaaaaaag tgcttgattt ttcatttatt attagtgatt cgtcagggaa
    17161 accggtaaat ggtagttcat tcattttgaa acataagaaa aaaacactta ctagaaatgg
    17221 tgtgccgttt tcagtacaaa gcgaccatca tgggaaagtc aagctggatc aattgcctgg
    17281 tggaacttat cgaatcgttt cagctgatga tcctaaagtt gctgtttctt ttggtattag
    17341 aaaagtaaaa caagagaaga tatacttttt tgaggggcga aaacttgtaa aagagcttca
    17401 aaaaaatggt ttttatttta aaatcaatga ataagtaagg agagaaaaaa tgatccgcac
    17461 aacaacaaaa ttgtctgcca aagaactgga atctgttcta tcgatttggc tgcatgctaa
    17521 cttggattct catgaattca ttccatcaac ttattgggag aatcaagtag aatctgttag
    17581 gaaagcttta ccagatgcag tgatttactt agcagaaata gatcaagaaa ttattggttt
    17641 tgctggggtg attgatcagt ttatcgcagg aatctttgtg aaaagggaat accggcaaca
    17701 aagaatcggt acgcaactaa tgaaaaaaat aaaagaaacc aataaaacac tgactttgac
    17761 agtctatcaa aaaaatcaat cagctattca tttctatctt tcacaaggtt ttacaattca
    17821 aacacagcaa aaagatgaag cagtaaatga ggaggaactt gtgatgaaat ggaatcaaaa
    17881 cgctgatttt tgatgaacag aataacgaaa taatgctccc agcctaattt tttgctggga
    17941 gcattatttc attttgaagt cactaattta actgtgtgaa tatgcagttg ttacaatctt
    18001 tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
    18061 ggaactattt ttccatttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
    18121 ttgataaacg gtgttcaaac ggctgcttct cggtaataag cccactagcc tagaaaatat
    18181 gaaaagctat tttctaacta gcgttcttat tactcaaagc ataacgctga tttcacaacc
    18241 tcttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaaca
    18301 tgaggaacta tttttccgtt tctttctcta atactcaggt cagaacgctg atttcacaac
    18361 ctctctttca caaccttatt ttactcgttt ataattttca tcgaagtatt ttccgctacg
    18421 gatatcatct ggcattccat cgtatggttc ttcgctaata acatcttcat tgtctatacc
    18481 tgcatcaccc atataagtga tcgtagcaaa ttcaggaaca agtaattttg gataagtttc
    18541 atatttttca cgagctgctt ccatttctgc gatatgttcg ttttggaaag taacagtgat
    18601 ttctggatgt tcttcaaccc atttagggaa aaagatcatt ccgtgaagtt tcttttcatt
    18661 tggcataaag tccaatgcca cgtctgtccc tgttggctct gtccaagtga ttttgaatac
    18721 gccgtccgtc aatttaacga tatcagcttc ttggtctttt acccaacggc ctttgaccat
    18781 tccgccatgg atacggtaat caacagtatg gtcatttttg gcataccatt catattccca
    18841 accattgtca tacgtataga taaagtgagt tccaataaaa tcttccaatt ctttaaattc
    18901 tttcatgttt aagcatctcc ttataaatta aaacatgtaa taaactacat gttttcgatt
    18961 actcatttat gatagaatgg atttaaagat ttgtcaagga gggaaaactg atggcaagaa
    19021 aaaatacact tcgatatatt ttgttagggt tattaagtaa gaagaagatg acaggatatg
    19081 aattgaatca atcgttcaaa aatgaaatag gagaattttg gcaagcaaaa catagtcaga
    19141 tttatcctga acttgcaaaa atggaagagc aaggaatcat tcagcatcaa gtagagatca
    19201 caggtgaaaa attggaaaag aaagtttatg aattaacaga agaagggaag gaacaattgg
    19261 cagagtggat tcatacacca accaatgaac taccggttaa tcgagacgaa tttgttctga
    19321 aattgtattt cgtcaaagac gtgaatgatc cggcattagt agaaattatt cagcagcagc
    19381 gtgaccttca tgaagaaaaa cgacttcatc tattagagcg aaaaaaaacg atctttccta
    19441 ctgaaaaaga aaaacatgaa aattacggac actacctgat tttagcgcat gcgatcaatc
    19501 gggaaacaga atatgcaaat tggctaacag atgtattaga agaaagtaac cgaaaaaaat
    19561 aaaaaaatat ccgaacagtg atggagaatt cctttctata tgaaagaaac gcagatcacc
    19621 gttcggatat tcttatgttc aaacaggcct tttttcaagt atttatgtag catctactta
    19681 ttaaaaagct tggtacttag gagattagtg gcgtttagag ttcttttaac aactgttcgt
    19741 agtcagcttt ttcatcgaca ggaaggagct tgtaagttaa ttcgtctgtc tcgttgtcat
    19801 gaatgatgta catcttagca ttctcttctt tgaagttacc gatttgtaaa atcgtatagc
    19861 gttcgtcgat caattcgata tctttgattg cttttgaaaa aactttacgg acatggtgca
    19921 ttttcttttg gcggatttct gccttttctg cagcttttga caaagtaaat ccgtcatcta
    19981 aatatttttt gatcagttct acttttatga ctgtaggtaa acgatatttt ctaggacaat
    20041 tcgcatcagc ttcgatcgaa cgaatgaatc ccttttgttc ccaatacctt aattgacgtg
    20101 gagacacacc tacgatatca cttaattcac tgatgccaac aaccaattga tcattttcca
    20161 acaaacgttt aagggaagcc ccaaccattt ttgtcactcc ttttgtcctc ttgcttccct
    20221 taattatatc cgtattaact tatgtgtcaa gtaggttgac aaatagcgaa tttaggacta
    20281 aagtattcta gtagtttcag aaaaaattca gaaagaagga gaaatatgag taacaaccaa
    20341 cccgtagata tttacggaaa accatataat cggacacttt tagttgttgt cttattaatt
    20401 ggtacatttt gtaccgtttt gaatcagacc ttattgacga cagcttttcc tacattgatg
    20461 aaagctttcg atatttcagc gtctgatgtg caatggttaa cgactggttt cttgctagtc
    20521 aacgggatca tgatcccaat aacagcatgg ctgatcaata aattcagttc aagaaattta
    20581 tatctttctg ccatgacgat ctttttgatc ggaacgatta cttgctttac agcacctaat
    20641 tttagtacat tactaatcgg tcgcttgatc caagcttgcg gcgtgggagt atcgatgcca
    20701 ttattgcaaa atatcatgct atctattttc ccgccagaaa aaagaggatc agcaatgggg
    20761 atgtcaggga tcgttattgg acttgctcct gctttaggcc ctacattatc tggttatatc
    20821 attgataact accattggcg tgacttgttt ggtatggtac tgccaatcgt tgttcttgtc
    20881 ttggttttag cattcttcct aatgaaaagt gttattccat tgtcaaatcc tagcattgat
    20941 attttatccg ctattttatc aacgattgga ttcggtagtt tactttacgg attttctagt
    21001 gtaggagatg acggctgggg aagcttgaaa gtaattggtt tcttagttgt tggtgtgatt
    21061 ttcatcgcct tgttctcatg gcgtcaatta catttggaac atccattctt ggaacttcgt
    21121 gtattcaaat catctacatt cacgattgca gctgttttag ctggtgtaac aaatatggcg
    21181 atggtagggg cagaaatggt cgtaccattg tatattcaaa atattcgtgg agaatcagca
    21241 ttccattctg gcttgatgtt gttgccgggt gcattgatca tgggggctat gatgcctgtg
    21301 acgggtgcga ttttcgataa atacggcgcg aaacgtttag ccatcacagg gatgttcatt
    21361 ttgacagcgg caactgcacc atttgctttc ttgacaaaaa cgactccagt tttatatatc
    21421 gtgatccttt atgcgatccg tatgtttggg atctccatgg tcatgatgcc ggtaacgact
    21481 tctggtatga atgcattgcc aaataacttg atcagtcatg ggacagcggt aaataataca
    21541 ttccgtcaaa tcgcaagctc gattggtaca gctattttga tcagtgtgtt gacgaatgtg
    21601 acaaaagcaa atatcccaag caaatctgta ttggataata cgccattagc ttataaagat
    21661 caggcaatca atgccacact gtcaggttac catgccgcgt ttatcgtagc gatcatcttt
    21721 gggattcttg gctttattat tgccttttta ttgaagaaaa aagaacctgt tctaatgaaa
    21781 gcgggggata atgcatgatt attggagttt tgattgtcag cttgcttgct tttgctttaa
    21841 cgaacgtgtt cgctaaaaaa acatggcaaa cctttttatc tttgattttc ggactaatct
    21901 tcgtcgcttc actaagtttg atcgtcgcta atttgtccaa tcactttgga atggaaaaag
    21961 taacggaaac aaaaacagaa aaaattgttt ccagtgctga ttcccaagga gcagatatgc
    22021 ttttgtataa agctttaggg aatggaaaag aaaaagtgta tctttaccga acaaatgaaa
    22081 aacaagaaaa accaaaagca actggtacgg ataacgaaac aaataaagta gaaaagacag
    22141 acggcgatgc tgaaaaagtg acgaaaacta cttattggga atacaaaaac gacatgtata
    22201 aattctggtt caatattgct gataacaatc acgaatatga cagccgagtg aatacattca
    22261 aaatcccaga gacatgggta gaattaagta cggaccaagc agccaagtta gctgaattag
    22321 tgaagaaaca gcaaagtacg atggaaagtg aagcaaaagc ttatgttcaa gacggtatgg
    22381 taaaagcaat gactgaaaat ccaaaaatga gcaaagcaga gcaagaacaa cgtacgaaag
    22441 ccttagctgc tgaattccaa caacaagcat tcgcaaaatt agtgaaagaa gcaaaaggcg
    22501 aataatattt tgaacaccat gtaggaggtt gtgaaaaagc tgctctgcat caagcagtaa
    22561 gaacaatcag ataaaaatag cactccatat ttttcatctg attgggctta gtgccgtaga
    22621 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta actctgagtc
    22681 acaaggagca atttcaaaaa acagcttctc atgtttgttg actattgtga cttgtgaccg
    22741 aggagttggc aggagaacac cgtttattcg aaaaaaggaa ctgcgacaag acttttgtca
    22801 cagttccttc ttttttttgc tatgctatac tagatgtaaa aaataggaga aataaaatga
    22861 gggaaattta ttcttttgaa tcaaaaatct atgcttccga agtcgacaag ggcggggcat
    22921 atattatttt tccgtatgat ataagaaaag aattcggtgc tggaagagtt aaagtccaag
    22981 caacttttga caatatgccg tatgaaggca gtatcgtgaa tatgggcgtg aaaaatccag
    23041 atggttcagt ctgctacatc cttggactac gaaaagatat tcgtaaaaac atcggaaaag
    23101 aaatcggtga tattgtagca gttacagtga agcaaaaata aaagaggttg tgacaaaaga
    23161 cgtgtcacaa cctcttgata aacggtgttc aaaagccagt ttctcggtag taagtcaaat
    23221 ttatgcaaaa atgtggaaac ccatgtttac aaaatttttc ttactactcg aaacgaagcg
    23281 gctttatcac aacctcttga taaacggcgt tcaatcagct gtccctcggt attagctcaa
    23341 aaacggcaaa atatgagaag ctatttttcc atttttttct ctcatactca ggtcataacg
    23401 ctgatttcac aacctcttct ttcataatgt tttttcacca tctttataat aaactggttc
    23461 tttgatatgg aaatctattt tttccaacca atctaaaatc ttgtaaccta actcttctga
    23521 catcggaaaa gaggcgttga tttgtgcatt ggagatatgg tcaacatgga taccggaagt
    23581 gatcgtacaa cttcccggaa cggcagtttt gatgatcaca gctatttttt cggctaagac
    23641 atcgtcttta tggaaccgac catcgtggct agggaaacgt acagtttgca tctctgtatc
    23701 acgagaaaat gtagtgactg ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa
    23761 taagtcttta ccgattactt taacagcagc ttggatcgtg aaacctagtt tggttacttc
    23821 aactatcgtt tctttttcca tattaatcac cctgccaacg ttttgaatgc tcatttacaa
    23881 ttccaaattg atccaaaagt ttcattgtat ggtgattaac aagatcttgg atcgtttgag
    23941 gctgattata aaaagcagga attggtggta tgatttgcac acctagtcgt gctaatttag
    24001 tcaagttttc caagtgaatg gcatttaatg gcgtttcacg aggaacaatg actaactttc
    24061 gttgctcctt cattgttaca tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag
    24121 agatacctgc gactgttttc atacttgccg gtacgatgat cattccatcg gtcaaaaatg
    24181 atccgctggc gacagcagca cctaaatctt tgtttgaata atggtaatca aacaggcttt
    24241 cgaattcagt tagtgataag tcggattcca gctttaagtt ctgttttgcc catggactga
    24301 tgattccgtg tgtttctatc atcggatatt cttttagttt cttcactagg ttgatggcat
    24361 aaatggtgcc tgaagcgcca cttacaccaa tcacgatttt tttcttccgt tgtgccaatt
    24421 gttccatttt ccgcactcct ttttatgatg tcaaaacagt tgcagatcat tcattatttc
    24481 aaatattttt cccaatcttc tacttcttgg aattttgcac gtacaaaatg ttctttcata
    24541 tcgaaaggaa ctgtaccgtc aaagatagtt tttgcactca tcccacggaa acgaatagag
    24601 ccaggattat attctggtaa ttctgatggg tccaatggat ggttcctcat tcctgggatc
    24661 acgatgatat ctttatctcc ttggaaacga gtgttgaaag tccaaactac atcgttcatg
    24721 tcgaaaatat caacatcttc atcgactaaa atgactgtct tcaattcttt gaaggctgat
    24781 aaagcaagaa tagctgcttg acgctgaata ccttcatccg cttcgttttc tttacggatt
    24841 tgcatgatgg tcatcaattt accaccacct gcaggaggat tgtagacatt tactactttt
    24901 cctggaatag ctttatcgac caaggcaaga atacttgctt ctgtaggaat accggccata
    24961 gatacgtgtt cttcactggg accaatcgtt gtttgcatga ttggattgtc ttttcggtga
    25021 gtaactgctt tgactttcac tacttgtaca gctggattag catccccagt atagccagga
    25081 aattctggca ttgcttttcc tgagttcgtg ttgatatctt cttgcattgt ttcatttggc
    25141 atgatttctg cttcaatgat gaattcagca cgagcaatac ccacttcatc gactgctata
    25201 ccatcaacga tttgaatagg ttcgttacgc aaagctcctg cacaccatag ttcattgtag
    25261 ccaagtggag tggtaggtgg ttcgaaagtc gtgccaatgg caattgctgg atctaaacca
    25321 atattgatag tgataggcat tggtttattc aaggcttcga attgtttttg gaagtgacca
    25381 atgtggcgtc cgccaggcat gatgtacata ccgatcgtat ctttatcttc tagaaccata
    25441 cgatggatcg ttacatcgct cattgttttt tctggatctg atcctaagac tacacccatt
    25501 gtgatgtatg gaccagcatc ttcaggagta tttgtaggtg caggcaaaat gttgcgaata
    25561 tcgaagtctg catcggtagc tagatgaacg acttcttgtg ctggagcgtc tgctttgtct
    25621 actttcacag gtttgattgg attttctaca gcgtctttca agaaacgacc aagtgttcga
    25681 taatcatggt gcaaaatttc acctacacgt ttacgactag ccattgttcc gatgttcaca
    25741 cgtgtgccgg ggaaaccttt gatattgttg aaagtcatag ctggtccttc ttgagtcgga
    25801 cgttttactg taccgccagc accgatataa cggtaaacac cagataattc tgcttcagga
    25861 tcgatttcaa tatctgtttc gtgatattga cctggaagtg tttttagttc ttcgatgact
    25921 tttcttaaat catacggttg atctgtcatg agagattcct ccttagttgt ctttacctat
    25981 ctatagtaaa aggtgaaaaa gaggaaaaca attcaacttg ttcggtaaag tattctacaa
    26041 tggaatggat gcgttttttt gataatagaa taaactatga tatacttatc actaaaagaa
    26101 agcgcatact tagtgttgga ggaaggaaag tgaaaaaaat gaaggagtca tttcctaaag
    26161 cagtaaaagt ggaaaatata gcgaatattt tgaaagttac atttgaaaac ggagaagtaa
    26221 aatacgtgaa atctcattgg acagaagaga taaccgatgc cttgcaattt ggaaaaaaag
    26281 gaagaggaaa aagaaaaaat cttctcgcac tttctcgtaa tatgtggatt gggactgaag
    26341 taacgattga agcagatgga acagtattca taaatggaaa agacaggtat acgccagaag
    26401 agttgtggta caaaggaaag aaaagtatac cagaattata aaaaataatt aaaaaaaaca
    26461 gcgcgaaaca aggaacatat tacttcctcg tttcttgctg ttttctacta tttcagcgct
    26521 cttatgactc aaatctaaat gtggttgtca caaccttatt ctgcacgcaa agcgatagcg
    26581 gcgtcttttt tcgctgccat tcgtgcagga agatgaccgc caagcatcgt caatatagta
    26641 gagacgatca ctagaataat cgcatggaca ggatttagtt gagcaacatt ttttagatcc
    26701 gtcatgttgt ataagatgct attgataggg aacgtagcta gccatgcaat aaacacacct
    26761 aatatcccag aagaaatacc taaaatacac gtttctgcgt caaatacgcg agtgatgtct
    26821 tttttacgtg cacctaaagc ttttagtaca ccgatttctt ttgtccgttc aatcacagat
    26881 gtataagtaa tgatactgat catgatcata cttgttacta aagagatccc tgcaaaagca
    26941 ataaggacat aagtgatagc atccatcaat ccgccagtca gttcagtcat cgttccagct
    27001 aaatcagtat aaatgatttt atcttcatct gatttgcctt tattgtaatc atctagataa
    27061 tccaagatct tttctttgtc ttcaaaattg ttcggataaa tcatgatact tgaaggcaag
    27121 ctgtctccac ctaaataact aagcaatgtt tgtttggcat tttcgtcaac tttttcagta
    27181 gttaagacat ttacaccact gtctctttga gctttgacga tttctgattc tttgttttcg
    27241 ttgacgattt gagtcgttaa ttgatcgcta taagcgattc ctggagataa gagattcatt
    27301 gtagaagaag atttcacgcg taggatcccc gagatcttca attcatcaga agcattctga
    27361 tacatcgcat cataatctgt attagggata aaattcccag ttggaagttt tgtatagtaa
    27421 gtattgttgt ttactaattt gaatgttgta ccaacgatat catcaaaatc aagtttttcg
    27481 tcttctttca catcaaatcc aagattcttc aatgcgttga tattagtatt attattgccg
    27541 tctacgatca gcacgacatc tgttgctgag gctggataac ttccggcaag taaagaataa
    27601 ttgtctttta aaaagttttc tttgctagtg tcaagctgtg tagggaaact tgacactccc
    27661 acacctgtca ttgctgacat cgtactagat aaagaaagag attccgtatc aggattttga
    27721 ttcgagaagc tgacaggctg tactttgcca tttacatcac gtaaaagatt gattcctgta
    27781 gtacgggtaa atccgatatt attacttaag ttaggatcga tgtctgttac gtaatcgacg
    27841 tatgtttgat caatcttatt cgtgtgctgt gctttatctt cttcgctgac ttttgcagta
    27901 atcgttttgc tgtctggata atccgctttg cttgcaccca atccggcatc gtcacgagtc
    27961 tggttagtcg ttaccttaga aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg
    28021 ttatcgattt gtttttggaa tccagaagaa agggacagaa cgatcgcgat tccgatgatc
    28081 ccaatactgg aagcaaagga ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg
    28141 aaggaaagct ttaacgctgt ccaaaaactc atctttgttc tgcgtaagtt aaattgatca
    28201 tcttttggtc gttcgatgtg aggatgggaa tctgttaaga tcttaccatc agaaaattca
    28261 atgatccgat ctgcgtattg atgggccagt tcaggattat gtgtgaccat gatcactaat
    28321 ttttctttcg ataactcttg gatcaattcc atgatctgga tacttgtttc tgtatctaac
    28381 gctccggttg gttcatcgca caaaaggata tctgggtcat tggctagtgc tcgagcgatg
    28441 gccactcgct gcatttgtcc accagataac tgatttggct ttttatgcat atgatctgtc
    28501 aagccgactc ggtgcaatgc atcttctgcc cgttttcgtt tttcgtcttt agaaacacca
    28561 cttaatgtca tcccaagttc aacgttttca ataatgccta gatgaccaat caagttgtaa
    28621 ctttgaaaaa tgaaaccgat agagttgttt cggtaggcat cccagtcact gtccttaaag
    28681 tctttagtag actttccgtt gattaccata tcaccggaat cataattatc tagtccacca
    28741 atcacattca gcatagtcgt tttaccagaa ccactaggac ctaaaatcgc tacgaattct
    28801 ttttgtctga aagcgacaga cacaccatcc aatgctttgg tggtggtttc gccgacttta
    28861 taatattttt ttatatcttt taattgaagc ataatcaccc ttctcccgta ttgaaatatt
    28921 cggtatacta ataagtatat cagaacaatg atcgtagtat acgaaataaa tgttaactga
    28981 agatgaacta caagaaaaaa tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc
    29041 gtattttagt agtagaagat gatccgcaat tacagttgct ttaccgttct gtgttggaac
    29101 gagcgggctt ttcagtcatc accgcattga atggagagga tgcgctgaaa aaactagaag
    29161 cgacacgtat agaattaatc attacagata tcatgatgcc gaaaatggat ggctatgact
    29221 tgctagactc attacgtagc accagatttg acacacctgt tctaatgatt acggcaaagg
    29281 ccgattttga ggataagaaa cgcgggttca aattaggagc agatgattat atgacaaaac
    29341 ccatcgatgt caatgagatg atcctacgtg tggaagcatt gcttcgacgt tcaaagatca
    29401 attacagcag tagcttgata atcggacaaa caaaattaga gctagatgct tatacagtgt
    29461 tacaagatca gcattcgttg attctgcctc aaaaagaatt tctgcttctg tataaattac
    29521 tgtcttatcc caacaagatc ttcactcgcc aacagttgat ggatgatatt tggggattag
    29581 atacgaatac agaagaacga acgatcgatg tacatatcaa acgtttaagg acccgttttg
    29641 aaaagacgga agattttcaa atcatcacag tacggggact aggctataag gcggttattt
    29701 aaagatgaaa aaaaagctat tttcacaatt atggatctat tttgctagta ttgtcttcat
    29761 ttcgatctta gtgacactgc tgtgttttct cggttttatt ttcttattgt ctcgacattc
    29821 tatctcgcct gctgaatcgg ctggaaaacc aacgttatac ccgttattcg tctttgcggg
    29881 gttcagtatg gtcgtgggta caggaatatc gatttttgtt gggcggcgta tcctgcatcc
    29941 aatcagtgcg ctagggacaa atatgagtct ggtagcaacg ggagattttt cgattcgaat
    30001 ggatgaacag cagaaagtag cagaagtaca gcaactgtat aaagatttca acgttatggt
    30061 ccaagaactc aatagtatcg agacactgag gaatgacttt gtgtccagtg tatcccatga
    30121 attcaaaaca ccactagcaa caatccaagg atatgttcaa ttgttacaag ccccaaatct
    30181 ctctgacgaa gaacgccaaa tatttttata tcggattatc gaaagtatca cgcagctttc
    30241 acaattaaca gagaacatct taaaattgaa caaactggaa aaccagcgta tccaactgga
    30301 aaagaaagag tatcgattag atgaacaaat cagagaagta atcgtttttt tgcagcctaa
    30361 atgggagaaa gaacagctag aattggatat cgaacttgct gcagtaaatt acacaggcaa
    30421 tgaagagttt ttgtaccaag tgtggctgaa tatcatggat aacgccataa agtacaatca
    30481 aatcaatgga cagattcata tcaaactatt tgagacagcc acagagattg gactggaagt
    30541 gaccgattca ggtgtaggaa tgaatgaaga gaccagagat cgaatgtttg aaaaatttta
    30601 tcaaggagat acaagtcgtc aaatttctgg gaatgggcta ggactttcac tggttaaaaa
    30661 aattctagaa ctgcatgatg gacgtattga ttatagcagt atcgaaggag taggaacgac
    30721 agcaatgata cggttaagta aacaatagga aaaagaataa aacccctaaa aattctattg
    30781 aaggttttta ggggttttta gtcgtttttt tgtgattagg gaaatgttct tacggttctt
    30841 ttggcataaa aatcgctgct ttcttattga actacgagaa atgttagagg tgtcattgaa
    30901 acaaggacat gatgcaagaa agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg
    30961 atagatggac agaaaatgta gggcttagtt ttcttcttga tttaaatact cccaaaatgt
    31021 atctgaagcc aaagaatgct caccgacttt acgagtaaat gcaagataac tctctgttgt
    31081 tggagaaata gggagaatca cagtattttc ttttaataga ttcttgactg ttttttccat
    31141 aacaatagca atccccatgt catttgcaat catattgatg atcaagtcga ttcttgctcc
    31201 tttataagag atttttggtt caaatccagc ttcgcgacac agatctagta caggttgata
    31261 caagttcgta gtggggccaa ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa
    31321 tttcttttta ctagctaaag gatgattttt aggtagaact gctacaaaat cgtctgtttc
    31381 agtaggcaac cactcaatcg aacttggtgg ggcctggaaa tttctagcaa agtggatcgt
    31441 gtcactatgt tcattggcgt cattgtaagt taattcgaca ctttccactt ctttcaactg
    31501 gactgtaaat tcaggatggc gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt
    31561 gatcattgtc ggtatcgtta agatatttaa tgaaagattg ttgtctttcg tataggtttc
    31621 aagcacttct tgcatagcat ggtattgttc tacgacattt tttacatatg ggagcaataa
    31681 gcttccagct tcggtcaatt cgatttttct atgggcgcgt tcgaataatt tgacgtctaa
    31741 ttctgtttct aacgaaagaa tctgtttaga aatatttcct tgggtggtga attgtcgatc
    31801 agcagttttg gtgaaattca ttgtttcggc taaatcaaga aaaacttcga atttatggat
    31861 atccatcata ttcctcctac ttattccaat aaagaatact ttaacacaaa aaatgaattg
    31921 gtgtaatctt ctctgtctgt tacaatcttt cttgcagcga aagcaataat tacagaaatt
    31981 tttctgattt ttttcgcttc gtagaaaaag agaggagttt tttgatgcca actggagttt
    32041 tgatcaatgt aggttccgtt ttgcttggcg gactaattgg cggattagta ggaaataaat
    32101 taagtgaaca ttttaaagca cagctgacga tggtttttgg tgtgtgttcg atggggatgg
    32161 gaatctattc cattgcgccg atgaaaaata tgccggctgt tatttttgct ttagtgatag
    32221 gaacagcgat tggtctgatt gttcatttag gtaatgggat aaataagggg gctgcgctga
    32281 tgcaagtacc tatttctaaa atcttcccta gtgaaaagct tggtatgacc cacgatgaat
    32341 ttatcagcac attagtgacg gtcattgttc tattttgtgc aagtggtacg gggatctatg
    32401 gaagtttaga ttctgggatg acaggagaca gtacgatttt gatttctaaa tctgtccttg
    32461 attttttcac agcagctatt tttgcgtgta atttagggta tgtcgtttct gtgatagcaa
    32521 ttccccaatt cattattttt tatattttgt tcttattggc aaaatttatt tatccattga
    32581 ctacaccaga catgatctta gattttaaag cttgcggcgg attcttgatg gtcgctaccg
    32641 gttttagaat gatcaacgtg aaaatgttcc cagtagctga catgatacct gcaatgatcg
    32701 ttatcatgcc actgagctgg atgtggacaa attggattat gccactgtta taataaaaat
    32761 caaagagaag ccattttttg ctgactaaaa aggatcaaat cttttagtca gtttttttgt
    32821 atttgacaaa ctgattttga aactatatta tatgttgtat aatatagatt gaaataaaat
    32881 attacacttg agaatagaag gtgacgtaat gcacatccag attccaactg ttcttcttga
    32941 tggtactgtg ctagcagcat taagcaagga agatatgtat gggtatgcct taacaaaaat
    33001 cgtacaagaa agtctctctg tcagtgaatc tacactatat ccggttttga gaagattgaa
    33061 aaaagaagga tatcttgaga cctatgatga accttttgaa ggcagaaacc gtcgatatta
    33121 ctcgttgaca ccaatgggaa gtcgtcattt agaagaaatt attgatgaat ggattgtttt
    33181 tcgagattct ttagaccggt tattggagga gagcacaaat gaatgaatat ttgaatgaag
    33241 ttgtggatca tcttcaagaa attcctgaag acgatcgttt tgatttgatc cagtattatg
    33301 aggaatattt tttagattct ggaaaaacat tagatcagat cattgatgaa tatggtacac
    33361 caaagcagtt tgccttaaaa ttaaaaatca gttacttttc agaagaagaa caagcagaaa
    33421 acgagttagt gaaaaatcca aaaagacaaa ttcgcctgat ttggttaatc attatcggtt
    33481 tatttgcttc tcctgtcctc attccactag cactggggtt tatattggtt attgggggac
    33541 tattggttgc tttactagcg ctcattttta gtatctatgt tttatgtatc agtatcttag
    33601 gtgttggcct attcatgatc atctcaggaa tcagtgtgtt tggacaatcg attctgagtg
    33661 gattattctt tatcggtgtc ggtctgttcg ccacaggaag cgcagtattt atttcgccac
    33721 tgttgctgaa aggaacaaaa gggctttttt atgtcatgat gaactttgtt aaatggatgg
    33781 gacgacgttt tattacaaaa agagatctgc attcagcggg cgtttgaagg aggacacata
    33841 aattgagatt gaaacatttt ttgattaccg gtattggtct gatgcttact ggtggcctcc
    33901 tagcaggtgc aacttataca ttaggcgctc aaaagagtct gacttgggat aatggtcctc
    33961 ggttgatcga tatggttact gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc
    34021 atgcagaaaa tcaaacagtt tcggtcatac gcggattgga ttttgagata aaaacgactt
    34081 atgataagac agaaaagcca gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag
    34141 tcaaaaaatc aacaggtgca atcattagtt tagaagacac aagtgcacaa atcactattg
    34201 ttgtccctgc agatcatgaa ttatctgaat tgacaatcga agggaataat agtgcaattg
    34261 atatcaacga agtatcaatc aaaaaagtga atactaagac acatgatggc tgggtatcgt
    34321 tcaatgaatt tacaggagga acaataactg cgaaagatga attaggctca cttcgattag
    34381 atgaggccaa tgtggataac ttgatcgtca caggtgaaaa cagcaacttc catctagaag
    34441 acagtttgat cagcaaagaa ggaaaagtca tcctaaacgg tggcaattta tctttgcatg
    34501 agatggaaaa cagtggctat actatccaaa cgtctggtga atcttatatt gaaaaagact
    34561 accagccaat aacaaatact ttgttagaac aaggacagaa aaagctaaag atcactgcta
    34621 aagacacatc gatcaatatt acgacggatc aagaggacaa tgaaacagat tgatcctgta
    34681 ctcaactgga tattttaaga aaaaacagca aagatccttc caatctttgc tgtttttcat
    34741 cgcttaacgc acataactat agtaaaataa gaaagaaaat atctaaaaag aaaacagggt
    34801 gaaagagtat atgaaggtag aagaaggtca aagagaaaag ttaaaaacag aaatggaaag
    34861 attacatact tatatcactc aactaagcca aaccttctat gatccagaca aagaaaaagt
    34921 gatggttaat tacccgaaca attcagaagg tcgccaatta gagcaagtgt accatgaagt
    34981 tttcaaacat ttattgacgg tgaagaaaga gctggattat tattctttac cgattatcga
    35041 tacaggaata ttgaagtatg atgggaagaa ggaacgattt atctttaaat ctgttcggga
    35101 agatttgcct ctttcagcag ggatggatct agaggtcttg gtagaagact attttacaga
    35161 agaaaagcac tgggttcgaa caaaattgga ttatttaccg caggcagccg gagggacaca
    35221 ggcaaggggt tggtacatca ctgaagacaa agagttagaa ctagaaggtg taatggcgcg
    35281 tattcgaaaa aaaattaaaa aagtaacatt agatatttat tgaaggataa aggagcgcag
    35341 gaagtagcat gagagaaaaa agagcatttt atatcaatgg ttatgcaggg attgtcggat
    35401 tggtcgtatt agctttagtt ggattgtttt tcttctattt aggtatgtgg caagcgaaag
    35461 tttgggcact ttttttaagt atttttcttt ggttgatcac gttattgttg ttaagttcgg
    35521 caactgtagt cagtcctaat caagcaaaag taattttgtt ttttggacaa tatttaggta
    35581 cgattcgaga aaatggcttt ttccttacga tacctttggc acaaaaaatg actgtttcgt
    35641 tgaaagttcg gaatttcaat agttctgttt taaaagtcaa tgatttggat ggaaacccca
    35701 tcgaaatctc agcagtagta gtctttaaag tcgttgatac agcaaaggca ttattcgatg
    35761 tagcgtatta tcaagatttt gtagaaatcc aaagtgagac agccattcgg catatcgcta
    35821 gccaataccc gtatgataca ttcaatgaga atgatttgac gcttcgagga aacacgacgg
    35881 cagtgtctga cgagttgcaa aaagagctgc aagagcgttt agcagtagca ggtgtcgaag
    35941 taatcgaaac acgcttgaac catttagcct atgcaacaga aatcgctagt gccatgctcc
    36001 aacgtcaaca agcaaaagcc attttgtctg ctcgacaaac cattgttgaa ggagctgtca
    36061 caatcactca aatggcctta gagcaaatcg aagatggaca agaaatcaat ttcaccgatg
    36121 atcggaaagt ccaattgatc aataatttac ttgtctcaat cattacagac aaaggaacgc
    36181 aacctgttat caatacaggc gacatgaaag accgtaagtg acatactttt tgtaagatag
    36241 gaagaaggag tttaggacgg attattgtct tgaactcttt ttttagttgt tttaaagaaa
    36301 atgacaaaat tgaaagaaga atgatagttg attcgaaggc agaattgttc gcttttttct
    36361 ataatcagta tataaagtaa gagagaaggg gaaaaagtga aaaaacttct gatcggtcta
    36421 cttgttttca tcgttgtggt acttgcaggg gtgaaaatcg cagattacgt agttatgggt
    36481 ggagaagaat actatgtaca aattacgaca gatggtacaa aaacagtttc aaaatcagac
    36541 tctggacaag aaacagtcaa ctatagatat atcctgcctg gctatacgaa agagggtaag
    36601 gaaaaagaac tggacttcaa gggacaattg actcgaccgc ttcgtaaagg agcatattta
    36661 aaaatcacct ggaacaaaaa taaaggtgtc acttcgtatg aagaagtcga aaaaaaagac
    36721 gtgccgaaaa cagcactgga aaaactaatt aaagggtagg gaaagaaatg aacaaaatag
    36781 tcgaagtaca gaatgtaaca aaagtatacg gaaaaaacaa tgagaaaaag acgcaagcat
    36841 taagcggcat cagcttcgat gtagaaaaag gcgaatttat tgggatcatg ggcgcttctg
    36901 gttctggtaa gtcgacactt ttgaatattt tatccacatt agataaacca acagatgggc
    36961 atatccgaat caatcaaaaa gatgtgacca cattaaaagg aaatcaacta gcagattttc
    37021 gtgcgaatga gattggcttt attttccaag atttcaattt attagaaagc ttgacagcac
    37081 aagaaaatat cgctgttcct ctttctttac aaggcgtcag accaaaagaa atcaagcaac
    37141 gtgtccaaaa aattgcagaa cgtttatcca tctctcatat tttggaaagc tatccttctg
    37201 agatttccgg tggacaaaaa caacgggtcg ccgccgcacg tgcattgatc acacaaccaa
    37261 cgattctatt aggggatgag cctacaggtg cgctggattc caaaagtgcc cgggatcttt
    37321 tagatacgat ggacgaattg aatacaaaag atcatgtatc gattcttctt gtgacgcatg
    37381 atccgttttc tgcaagttat tgccaacgta ttttattcat caaggatggc ggtatccatc
    37441 aagaagtgaa acgtggagat caaagccgtg attccttcta tcgagaaata ctatctattc
    37501 ttggaaactt ggaacagtag ggggagattg actatgcttt ggaaattatc acttactggg
    37561 atcaagagcc gtttaagaga ctacttggtg ttattctcag gattagttat ggcatctgct
    37621 attttttaca tgtttgaatc aatggcaagc aacgaagctt ttttgaaaag taatacgatc
    37681 gttgcggcta cagttatcat tttccgattc ggttctgttt tactagggat catcacattc
    37741 gtttatattt tatatgcaaa ttcctttttg atgacgatgc ggcaaaaaga ctatgcaatg
    37801 tttatgatgc tgggagcaaa aggcagaaaa atcgcacaga tgattttttc tgaaacattc
    37861 ttagtaggga tcgcagctac tttagtgggt tcagctgtcg gtgtaggatt gacaagtatc
    37921 gtccaccgat tattagtgga tcagttagat ctcaatattt cgcattttac accttttagt
    37981 attcacgggt tgttgatcac tgttttgttt tttgctgttt tgtttttatt agctgctgtt
    38041 gtcaatgctt tttcgattgt caaaaaaccg attttagcat tgattcgtgc ggatcagaca
    38101 ccaacaagaa tcaagcagca taaattcttg tttttccttg aagtagtact gggaattgct
    38161 tttctaggct gcggttacta tatgatgaaa cacgtgggga cctatcaagt ctttggtata
    38221 ggggtagcct tagttacgat catattagga acttatttca ttttccattc tgtgatcatt
    38281 ttctttttat cattactaaa gaaatcagaa cagatttcat tgaaaaaatt gaacaatttc
    38341 acattgtcac agttaaactt tcgtattcgt gaatatacac aaatgctctc aatggtcgct
    38401 atgctgtttg ccttggcact gggcgctttg acggtaggat taggttttaa aaatgaaatt
    38461 gcagacttga cagaaaacaa ttctggatat gatctagtga tgaataatcc tcgggcagaa
    38521 gatcagcaaa aaatcaaaga actttcacca acactttctg cttcttatac tcaaaaagaa
    38581 gatgcgggaa cgatttacta taatgcaagt gaatttgcgg ctcaaccatt gatcatgata
    38641 aaacatgaag atggaacacc gccgaagatc acgaaagaaa aagttcctgc taaaaaaatg
    38701 aatgaactga ctgtacaaga ggaacttcga agctatgaat tgccggaaca gcaagaaaaa
    38761 gaaatcaagg tagtcagtca atcagaattt gatcaactga acttgccaca atccacttta
    38821 cagctagttc aggtaaaaga cttccaagca aacctgaaac caatcgaaac acttgttgct
    38881 caaaataaaa tgaacaatcc atctttgcaa aaagtagagt acagcaacca gaaatatgaa
    38941 atgtatgaag tgtataatgg tctattctct ggttttgaat ttatgggatt cttcctaggt
    39001 ttagctttct tgaccatgct tgctagctgt ttgatgttca agatcttgtc aggaagcaag
    39061 agtgacattg ctcgttatga aatgcttgaa aagataggaa cacgacgcgg gttgcttaaa
    39121 caatcgatcc gtagagaagt aggcgtgtta ttcttagcac caggaatctt aggtgtagtc
    39181 catgtcttat tcggattaca aatgtttggt ctattcatgc aaaatccata caaagatatt
    39241 tgggttccgt ttacgatctt cttcgtattg tatttcatct actatgtact gacgacatgg
    39301 ttatatactg gaatcgtatt gaaaaagaga ttgtgaaaaa gctgtcctgc atcaagtaat
    39361 aagaacagcc aagcaaaaat agctcctcat atttttcgtt tggttgggct tattaccgca
    39421 gatgcaagct tttgaacacc gtttgttcgg aaaaagaggt tgtgacaata tttttgtcac
    39481 aacctttttt tcacggtctt ttattagaat atagtaaatt ttttctcgtt actcacggaa
    39541 taagctgtta agaaaacggt ttccatgtat actggaatca gtgcaaatga aagaaaagag
    39601 ggtgcaagga acatggaaat caatgaacaa aattttggcg aaaactatca cttgattacg
    39661 atcacgaatc aaaatgggac atctttgtct gtcagtgatc ttggggcacg ggttgttcat
    39721 tttcaaacaa taatcgataa tgaaaaacga gagttgatct taggttttga tgaagcaaaa
    39781 gaatatctag aaaaagaccc ttatatcggt gcttcaatcg gacgtatggc tggacggatt
    39841 acgaatggca cattcaaatt agatgatgaa acgttcgaag taaaaacaga cccggacact
    39901 ggtcattgtt tacatggtgg tgcacctagc tttgaagcca agcagtggca ttatacagtc
    39961 atcaatggtg aaaacgaagc atcggtcatt ttctatcaga ctagcccaga tatgggaaac
    40021 ggatttcctg gaactttaga tgtagaagta cggtatacgc tgacgaacga tgatacatgg
    40081 cgagtgacgg tccaagggat cagtgaccgc aaaacagtat tcaatcctac aaatcatgtc
    40141 tatttcaatt taaccggtga tccagcacag tcaatcgacg aacatatact ttggttgaat
    40201 agccgattat ttgctacgct caataaagat acaacgccta caggagaagt agtttcagta
    40261 agtggcacag catttgactt ccaacgtcct aaaaaattag gtgaggtctt tgccagtgat
    40321 tttcgccaat caatgatagt gggcggcatc gatcatccat ttttgttgaa aaaacaatct
    40381 ggtttactaa atgctgcgga attgacaagc ccagatgaaa aagtatcgat ccaagtaaaa
    40441 acagatgctt caggaatcgt gatttttaca gcaaattttg gagaagatgg accggaaatg
    40501 cgcgggaata aattagtaaa tcatggcggt atcacatttg agactcaaga actgccagga
    40561 gcggagcgac ttgaaagttt tggagatgtc actctggaac ctaaccaagt a
//
LOCUS       DAHUWM010000003        39031 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHUWM010000003 DAHUWM010000000
VERSION     DAHUWM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.003"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            18..899
                     /locus_tag="NK788_000078"
     CDS             18..899
                     /locus_tag="NK788_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768459.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="NK788_000079"
     CDS             912..2522
                     /locus_tag="NK788_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCH6768460.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="NK788_000080"
     CDS             complement(2599..3258)
                     /locus_tag="NK788_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCH6768461.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="NK788_000081"
     CDS             complement(3541..4206)
                     /locus_tag="NK788_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCH6768462.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="NK788_000082"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="NK788_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCH6768463.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="NK788_000083"
     CDS             complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="NK788_000083"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCH6768464.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5971..6417)
                     /gene="dhaM"
                     /locus_tag="NK788_000084"
     CDS             complement(5971..6417)
                     /gene="dhaM"
                     /locus_tag="NK788_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCH6768465.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="NK788_000085"
     CDS             6520..6840
                     /locus_tag="NK788_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCH6768466.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..7821)
                     /locus_tag="NK788_000086"
     CDS             complement(6952..7821)
                     /locus_tag="NK788_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6768467.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7917..9220)
                     /locus_tag="NK788_000087"
                     /pseudo
     CDS             complement(7917..9220)
                     /locus_tag="NK788_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9217..9726)
                     /locus_tag="NK788_000088"
     CDS             complement(9217..9726)
                     /locus_tag="NK788_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768468.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9901..12036)
                     /locus_tag="NK788_000089"
     CDS             complement(9901..12036)
                     /locus_tag="NK788_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6768469.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12119..13774)
                     /locus_tag="NK788_000090"
     CDS             complement(12119..13774)
                     /locus_tag="NK788_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6768470.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13776..14090)
                     /locus_tag="NK788_000091"
     CDS             complement(13776..14090)
                     /locus_tag="NK788_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCH6768471.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14260..14520
                     /locus_tag="NK788_000092"
     CDS             14260..14520
                     /locus_tag="NK788_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCH6768472.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14534..14848
                     /locus_tag="NK788_000093"
     CDS             14534..14848
                     /locus_tag="NK788_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCH6768473.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15578..16132)
                     /locus_tag="NK788_000094"
     CDS             complement(15578..16132)
                     /locus_tag="NK788_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCH6768474.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16258..18186)
                     /locus_tag="NK788_000095"
     CDS             complement(16258..18186)
                     /locus_tag="NK788_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCH6768475.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18492..19208)
                     /locus_tag="NK788_000096"
     CDS             complement(18492..19208)
                     /locus_tag="NK788_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCH6768476.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19209..21032)
                     /gene="glpO"
                     /locus_tag="NK788_000097"
     CDS             complement(19209..21032)
                     /gene="glpO"
                     /locus_tag="NK788_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCH6768477.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21046..22542)
                     /gene="glpK"
                     /locus_tag="NK788_000098"
     CDS             complement(21046..22542)
                     /gene="glpK"
                     /locus_tag="NK788_000098"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCH6768478.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22678..24138)
                     /locus_tag="NK788_000099"
     CDS             complement(22678..24138)
                     /locus_tag="NK788_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768479.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24128..25423)
                     /locus_tag="NK788_000100"
     CDS             complement(24128..25423)
                     /locus_tag="NK788_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6768480.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25755..26432)
                     /locus_tag="NK788_000101"
     CDS             complement(25755..26432)
                     /locus_tag="NK788_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCH6768481.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26462..27553)
                     /locus_tag="NK788_000102"
     CDS             complement(26462..27553)
                     /locus_tag="NK788_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCH6768482.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27654..28088)
                     /locus_tag="NK788_000103"
     CDS             complement(27654..28088)
                     /locus_tag="NK788_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6768483.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28672..30129)
                     /gene="ascB"
                     /locus_tag="NK788_000104"
     CDS             complement(28672..30129)
                     /gene="ascB"
                     /locus_tag="NK788_000104"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCH6768484.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30126..32021)
                     /locus_tag="NK788_000105"
     CDS             complement(30126..32021)
                     /locus_tag="NK788_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6768485.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32079..33854)
                     /locus_tag="NK788_000106"
     CDS             complement(32079..33854)
                     /locus_tag="NK788_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCH6768486.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33904..35400)
                     /locus_tag="NK788_000107"
     CDS             complement(33904..35400)
                     /locus_tag="NK788_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6768487.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35594..37216)
                     /locus_tag="NK788_000108"
     CDS             complement(35594..37216)
                     /locus_tag="NK788_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCH6768488.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37665..38978)
                     /locus_tag="NK788_000109"
     CDS             complement(37665..38978)
                     /locus_tag="NK788_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCH6768489.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT        10995 a         8360 c         6725 g        12951 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atccgcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7201 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7261 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7321 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7381 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7441 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7501 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7561 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7621 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7681 agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7741 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7801 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     7861 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     7921 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     7981 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8041 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8101 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8161 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8221 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8281 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8341 cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat cttcaacgtc
     8401 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8461 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8521 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8581 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8641 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8701 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8761 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     8821 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     8881 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     8941 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc ttcgatcaca
     9001 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9061 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9121 catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata gacaaacaag
     9181 tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca ccccattttc
     9241 tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga cagaataaaa
     9301 acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt cttcaagtaa
     9361 ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta cttttttgta
     9421 tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat
     9481 caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa aagcgtacaa
     9541 ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata taaaaggaag
     9601 aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta accagtaatt
     9661 accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc ttgttacttt
     9721 tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat acatttcatt
     9781 tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac atttctcatc
     9841 agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt tatctcttaa
     9901 ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg ccgtgtacat
     9961 ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga ttacatgatc
    10021 aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta atccatatga
    10081 atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg tgataactgc
    10141 cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca attcttcaga
    10201 ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata cgatgtcgat
    10261 cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc tagaacggta
    10321 actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac aacgcataat
    10381 atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa
    10441 gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat aatcttgtac
    10501 ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg
    10561 aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag cataagctga
    10621 gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc atgccatttc
    10681 ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg cataagctac
    10741 aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact gcctatcttc
    10801 tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca aaagatgatt
    10861 cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca atttaggatg
    10921 agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg tttttccatt
    10981 tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg attgttttag
    11041 attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat tttctcccgt
    11101 gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct gtgaaaccag
    11161 ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga cattcgaaat
    11221 atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg tcatcgtatc
    11281 tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt taccacttcc
    11341 tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg tgttttgttc
    11401 cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc ctaagacttc
    11461 taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca aacgatctct
    11521 ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt gacgaaccgt
    11581 ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg aacgccaatc
    11641 ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat aaaaggattc
    11701 ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac ttttcaataa
    11761 acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca tctgatcgat
    11821 ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa
    11881 attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta tttttgccgt
    11941 atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca cgttcttcag
    12001 atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct ttttttaaag
    12061 tggtggaccc gattagttta cctcttttaa gagtcgaatg caagcttttt aacgagtatc
    12121 atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa aatgcaccat
    12181 caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga tagctacgta
    12241 atccgctatg acagctgacg atatattctt gttggctgtc taattcattt aatcggtccc
    12301 gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc aattcttctg
    12361 ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat tcatgccatt
    12421 gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc atgtttactg
    12481 gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt aaatcgaaga
    12541 tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc acaccttttg
    12601 ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc aacttcagta
    12661 aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca tgaatgactt
    12721 caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat gcggcagtca
    12781 ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga gaaattccgg
    12841 aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga gaaataagtg
    12901 catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca taaatatcag
    12961 gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct aattcgattc
    13021 cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata actaaatcag
    13081 cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca aatccagcga
    13141 cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct ataaaagctg
    13201 ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc atgacttcta
    13261 aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca ccaacgatca
    13321 cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct ggtacatttc
    13381 taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca actggttttg
    13441 caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc tcatgtacga
    13501 cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga cgcacatcta
    13561 aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc cgttcactga
    13621 tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac ggtccttttt
    13681 caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca gcagacattc
    13741 cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac tgttcgatca
    13801 aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt ctttaattct
    13861 ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt cttttctttg
    13921 tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt tatatgcccg
    13981 ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa aagcttttcc
    14041 tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat tttctgcacc
    14101 tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt attaatatgt
    14161 caaccagaat gactcttcat ttttttcact actttttata tttgcataaa ataccgataa
    14221 ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga taataaaaat
    14281 atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa aatgatcgaa
    14341 gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc tagtattgat
    14401 cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa acctgatagt
    14461 aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat taaaaagtaa
    14521 aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac
    14581 agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg tcgtatctgt
    14641 aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct tagatttgca
    14701 aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt cagaaagttc
    14761 tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg aactgcaatc
    14821 ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag tgatcagctt
    14881 caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt
    14941 gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    15001 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    15061 ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa atagctcctc
    15121 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttaaggaa
    15181 gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggattt
    15241 ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat accgtttaag
    15301 gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagc
    15361 tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    15421 ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt caaaaacaac
    15481 ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg agtctattta
    15541 tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga aacatctttc
    15601 ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa
    15661 tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc atgccaacat
    15721 agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg atatttttag
    15781 atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat agaccaaaca
    15841 acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt
    15901 tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat ctccgagcaa
    15961 tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct gctatataaa
    16021 atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg aatatcgata
    16081 aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc attttatcgc
    16141 cctacctcct cagcactctt ttctttttat tttagcttac acggggaata aaaccaatga
    16201 tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt gagataatca
    16261 cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt cttttactag
    16321 tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac gtgttttata
    16381 atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac
    16441 cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata catcaatcgt
    16501 atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga caaatatccg
    16561 atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg taactgctgt
    16621 cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt aacgctctgc
    16681 ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag cttggctttc
    16741 tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat cgaaagccat
    16801 agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc cataatgggt
    16861 cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga tcgctagtgc
    16921 agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca acgtattcct
    16981 ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc
    17041 attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga cagcaagctc
    17101 tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca ataaaccgac
    17161 tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca agcctcgata
    17221 gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc
    17281 ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat tgatttctcg
    17341 gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga tcaataaatc
    17401 gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt gatattcttc
    17461 actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa tcacagttgg
    17521 ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat ctattttatt
    17581 tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa tcattgcttc
    17641 ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg actggatttc
    17701 tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact
    17761 gactttatta ttttttattt tctggtcaat caactcttct acatttatca aatcaggaag
    17821 atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa aaaactttgt
    17881 gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg gctgcagaat
    17941 caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca taaatggaac
    18001 atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac
    18061 agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat agtttccaat
    18121 tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa tcatttcgtt
    18181 gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa gggcctagag
    18241 ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat tagaatacaa
    18301 agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact ttgtttattg
    18361 gctattcgac ttactgatta gataccagct acactcacaa aacatacccc attcttctag
    18421 gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa actttagtat
    18481 gttttataga tttacaccat taatgaatag acacctaccg cgataattgc tccgatcatt
    18541 ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt agctattggc
    18601 aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc atatcctgtt
    18661 ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa tacgttcgtt
    18721 ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac aaatgtacca
    18781 attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt tgcaaacgtt
    18841 cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga aagccaaaca
    18901 agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac gacaagattc
    18961 caactaattg cccctgtcat cgccatagct agagagactg caggattcaa atgagctggg
    19021 cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc aaatgcgatg
    19081 acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc acacacacca
    19141 tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg agtcatatca
    19201 ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat caatgcttct
    19261 aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc agcaattgca
    19321 tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag atgatttgta
    19381 cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc ataacgtaac
    19441 cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc taattcaaag
    19501 atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc ttcctctaaa
    19561 ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt agttggatcg
    19621 aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc tattccgtat
    19681 tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt acggtaatcg
    19741 gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa agaactacct
    19801 ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt caacacgtct
    19861 tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc gacaacttta
    19921 ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga accagaatta
    19981 ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa tgtgatattt
    20041 gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc ttgtgtgact
    20101 ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta agtcttgttt
    20161 tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa gtatgtgggt
    20221 tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt tggacgcatt
    20281 ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca aggaccactc
    20341 gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag gtcacgagct
    20401 ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat tttacttact
    20461 agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg ggcatcattg
    20521 ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa aaacggttct
    20581 ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc tgtcacattt
    20641 gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa ggttgttgct
    20701 ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg tggggcaata
    20761 ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc aaatgtcttc
    20821 agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa
    20881 tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac tgccacaccg
    20941 gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg cattttttga
    21001 atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt caaatgcttg
    21061 ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct cttcctccat
    21121 ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg tatctttcca
    21181 ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc
    21241 tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat attcattgtt
    21301 tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt
    21361 atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa tgatatcttc
    21421 ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat cccaataagg
    21481 agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg tagagcgttt
    21541 cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc tcagccattg
    21601 aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct ttccatttat
    21661 gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt caccggtatt
    21721 catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt cgaatgccat
    21781 ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg cttcgcttcc
    21841 ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga cttttggtaa
    21901 catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta gctgatggat
    21961 attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt gtccatcggt
    22021 cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt tttcagctcg
    22081 ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg agaaataggc
    22141 atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat tttttaagtt
    22201 atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga ttggacgtcc
    22261 tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa tcccagcaat
    22321 ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact gtacagaatt
    22381 ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt gcgtgaactc
    22441 tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc gtgagctagt
    22501 tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc cctccattat
    22561 atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa gaaactgtca
    22621 aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat tttttaatca
    22681 attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt cgtaaggtga
    22741 cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa atgtgatcac
    22801 caaatcatat tctttacctt cttcataact tccaatctca ataccgccga ttcccctcag
    22861 ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt ctaaatcgat
    22921 tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat tggcatactg
    22981 gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt gctgcaccgc
    23041 tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt tctgctgctg
    23101 ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa accattcatt
    23161 gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca aacgacgagg
    23221 acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg ttggtaactt
    23281 tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg aaaaagaaac
    23341 aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg ttcgactaag
    23401 atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat agagtaattt
    23461 cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt tcgtaatccc
    23521 gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta atacacggtc
    23581 caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc
    23641 ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct cttctccgta
    23701 taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca attctttgat
    23761 ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa cgatggatac
    23821 ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct gcaataaaaa
    23881 caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct gatactcatt
    23941 tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat cttccaaata
    24001 ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga tagaggctgt
    24061 tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt ttctttctaa
    24121 taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata tctggcacac
    24181 ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc catccttctt
    24241 ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt tggagctgtg
    24301 ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg ctttgattta
    24361 ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa aaaatgctct
    24421 cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg ccgactggac
    24481 gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca tttcgtgctg
    24541 tacggacagc gttatttact agagaaacat acattttttc atttgttgga cgaaaagtta
    24601 cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag tagtcatcta
    24661 cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga ttgtcggggc
    24721 gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc gtctctacaa
    24781 tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt gaaagccctt
    24841 gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa cgaaaaagaa
    24901 ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac ccttcagcta
    24961 tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc atgatctttt
    25021 gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt gattctgcag
    25081 aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca aaatccaatt
    25141 tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc ttttctgtgt
    25201 aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa agttgttttt
    25261 cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat cctaacttcg
    25321 attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga taaatagccg
    25381 catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt cctctttctt
    25441 ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa ctcgctttca
    25501 accttatgat acatagcgat gcagcagaat caaccctctc catcataccg tgttctaggt
    25561 ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg aagagatgtt
    25621 tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc tgtccaaata
    25681 ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag ttagaataga
    25741 cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa ttgatgagca
    25801 gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc tgaaaaagct
    25861 gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa gaatggtact
    25921 tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac tgtacgtcca
    25981 gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac accgtgacaa
    26041 acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc aagatctatt
    26101 gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc tgcataatca
    26161 tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct gaaatcttca
    26221 tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg ttctaaaggt
    26281 acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac taatacatca
    26341 taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt ttcgtacttt
    26401 tctacatttg ttaatacgat caatactttc attttcccat cctctttcta taaaaattgc
    26461 gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa agctctttat
    26521 atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat aatttctctg
    26581 cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca tatttcaata
    26641 cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg atctttcctt
    26701 gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca gaacgagcta
    26761 ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca cgtaatcgtt
    26821 caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct tctggtgaga
    26881 aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag aaaaccgttg
    26941 ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat tgatcctctc
    27001 gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct ccatgcaact
    27061 cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat gcttggattg
    27121 tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga cgttttgctg
    27181 caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt gtcattcgtc
    27241 caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct ttcgctagct
    27301 cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc cagccttttc
    27361 cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta gaacgttgtt
    27421 tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca
    27481 taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta attgattgat
    27541 caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc gagaatcatc
    27601 tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa attttatttt
    27661 ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact tgcttcattt
    27721 cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac ttccttagca
    27781 aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct aactgttttg
    27841 acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag
    27901 agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat caataaaata
    27961 aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa tttattcaac
    28021 atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt ttcaattggc
    28081 ttttccattt catcccctcc attggcgtac atatcatagt atgattttta ttttttgtca
    28141 aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa gtagccatct
    28201 tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt
    28261 gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat
    28321 ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt tatatcagag
    28381 gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    28441 tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg tttattgaga
    28501 ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa acagcttctc
    28561 atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac tgtttattcg
    28621 taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt attataggat
    28681 ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct ttaaccgttt
    28741 ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac gttttgacat
    28801 ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac caaataaatc
    28861 tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat agtcaatgcg
    28921 gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga gtccgttttc
    28981 agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa ttctcaaacc
    29041 cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt taattcctcc
    29101 agttattaag tttccttgga ccccttccca attttctgga tgagcactga ctgcactaga
    29161 catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt cttcatcacc
    29221 tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat acgctggata
    29281 atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct caattgtttt
    29341 ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga tcatacaacc
    29401 gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac ttgctataaa
    29461 ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt cttcaattac
    29521 tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag tcatccaata
    29581 ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat
    29641 aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca tttcataatg
    29701 ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca cttgatcata
    29761 aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca
    29821 tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga tgtcttcttt
    29881 aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc tgttgggatc
    29941 ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc atttttcttt
    30001 aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt catctacatc
    30061 ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc cttctggaaa
    30121 ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt tttcttatga
    30181 ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt ggtcggatca
    30241 tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc tttacgtact
    30301 ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac tgtatcaata
    30361 ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc atgtttcgtt
    30421 ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc cgttggaatg
    30481 atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac ttcagataaa
    30541 tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga ttcttcttcg
    30601 atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat cgctaagaaa
    30661 taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga caaaatactt
    30721 cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc aaatatagct
    30781 ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc ataaacagca
    30841 ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact ttttactttc
    30901 ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc tacggcagtc
    30961 atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt cattactggt
    31021 acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc gataacagca
    31081 ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat cagctgactt
    31141 gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac gatgatacaa
    31201 atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca
    31261 atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt ataccttgct
    31321 agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc actgacaatc
    31381 gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt attgaacttt
    31441 tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc agctgctgca
    31501 ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt acataaggcg
    31561 agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc aaaaacagcg
    31621 gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct ctttccacta
    31681 gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc atttactgca
    31741 tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac accttctagt
    31801 tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg taaccttgtt
    31861 gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac aatatctttc
    31921 gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt agcaatcctt
    31981 cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt accccttata
    32041 atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa atcactcact
    32101 gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc
    32161 ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt gatttttgtt
    32221 tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg aagcataact
    32281 aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg gatttttatt
    32341 tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc ttcaactatg
    32401 gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc tagaacacta
    32461 ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt caaaattgca
    32521 tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc tatccggcat
    32581 tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt caaaatacta
    32641 attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt caacgcttcg
    32701 gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg aaaatgtatt
    32761 gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat cttttgtaca
    32821 aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt gatatgggtt
    32881 agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa acgaataatc
    32941 gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc attaatcatc
    33001 aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt ggaaaaagaa
    33061 tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat aaataatgta
    33121 tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac gagataccga
    33181 gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag aacctctttt
    33241 aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg ttgaggcaac
    33301 cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct ttttgaattt
    33361 tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa tttaggattg
    33421 cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt tgaaagaaag
    33481 aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa gtacgtcaca
    33541 atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac ctcttgtatg
    33601 atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg tttatttgac
    33661 tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt ttggatcgtt
    33721 ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg attcttattt
    33781 cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt acacctcctc
    33841 tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc agaggtttaa
    33901 aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga taatttgttt
    33961 ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat cattttgtct
    34021 atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg atacgatatc
    34081 gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg cctcagcaat
    34141 ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc gattcaattc
    34201 atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta attcataacg
    34261 gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc aactccaagg
    34321 ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc cagtatctac
    34381 gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg caatataatc
    34441 tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg cacagtcttc
    34501 ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga catcggagaa
    34561 aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat cacagtcata
    34621 tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat ttggatcgat
    34681 tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac aggcttgata
    34741 aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag ggaatgtaac
    34801 caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa aggtcatcca
    34861 atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc
    34921 gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg gggtttcata
    34981 atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga ttagtctgtc
    35041 ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa atattcgtgc
    35101 ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac gaatatcctc
    35161 ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg attcgtctaa
    35221 agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct ggttcatgat
    35281 cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg caccttcaca
    35341 ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt cctgatccat
    35401 aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat tcatcaaaac
    35461 aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac tcttcatttt
    35521 taatatttat atataacaaa tacataagac gatccccgaa agaaaacact cgttaaccat
    35581 ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt aaatcaactt
    35641 tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa gcttcacgtg
    35701 gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat tgctggtctt
    35761 catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat tcatagtctc
    35821 cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac atataaaaaa
    35881 cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt ggattgactc
    35941 gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat tgcataggcg
    36001 tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct tttgtaaatc
    36061 cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct tccgcgatat
    36121 catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa ggggtgcctt
    36181 tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac acatcgtcat
    36241 ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa ctattccatc
    36301 cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca gagatactca
    36361 agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg tgttcgaatt
    36421 ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg atttcggtag
    36481 ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt gcttgattca
    36541 tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta tccggttctt
    36601 tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg atccagaaat
    36661 tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa tcaggttgac
    36721 gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc caagcagaac
    36781 cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca cgccaaatat
    36841 aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac ggatgttcat
    36901 cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc tttgcttctt
    36961 caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct tgataattac
    37021 tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc caaattgcat
    37081 caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa tctccaatac
    37141 catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact gaatggtgcc
    37201 accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt tgcttaatca
    37261 tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt gcaccttgtt
    37321 atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa
    37381 ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac ggtgttcact
    37441 cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat tttttcgttt
    37501 ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa cggtgttcac
    37561 tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt
    37621 tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt cacattcaat
    37681 atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga tacttctttt
    37741 cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc aatcccatct
    37801 gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat cgcaatctct
    37861 aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac cataatcaat
    37921 gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa caagcctaac
    37981 aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac ggcgatacca
    38041 gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc cgcaagcata
    38101 gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc tttcatgaaa
    38161 cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt cacaagcaag
    38221 aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg ttttaatgca
    38281 actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg cattggaatc
    38341 aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat gccacctggt
    38401 acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa ccaagtagaa
    38461 ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat gatcccacct
    38521 tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc atatttcaaa
    38581 tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat caaaataaaa
    38641 atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc cattcctaaa
    38701 caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc aattggataa
    38761 cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt cgcagtaaac
    38821 actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa taagatataa
    38881 gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt ggtgttcaat
    38941 ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc taaatccgaa
    39001 cattataaat aaagtccgtt aaaatttcga t
//
LOCUS       DAHUWM010000004        34507 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHUWM010000004 DAHUWM010000000
VERSION     DAHUWM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.004"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(236..2128)
                     /locus_tag="NK788_000110"
     CDS             complement(236..2128)
                     /locus_tag="NK788_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768490.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2125..3852)
                     /locus_tag="NK788_000111"
     CDS             complement(2125..3852)
                     /locus_tag="NK788_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768491.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3868..4518)
                     /locus_tag="NK788_000112"
     CDS             complement(3868..4518)
                     /locus_tag="NK788_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCH6768492.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4722..5018
                     /locus_tag="NK788_000113"
     CDS             4722..5018
                     /locus_tag="NK788_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCH6768493.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5190..5810
                     /locus_tag="NK788_000114"
     CDS             5190..5810
                     /locus_tag="NK788_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCH6768494.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5902..7068)
                     /locus_tag="NK788_000115"
     CDS             complement(5902..7068)
                     /locus_tag="NK788_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6768495.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7080..7433)
                     /locus_tag="NK788_000116"
     CDS             complement(7080..7433)
                     /locus_tag="NK788_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCH6768496.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7561..8583
                     /locus_tag="NK788_000117"
     CDS             7561..8583
                     /locus_tag="NK788_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCH6768497.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8602..8763
                     /locus_tag="NK788_000118"
     CDS             8602..8763
                     /locus_tag="NK788_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768498.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9022..9456)
                     /locus_tag="NK788_000119"
     CDS             complement(9022..9456)
                     /locus_tag="NK788_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768499.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9566..11425)
                     /locus_tag="NK788_000120"
     CDS             complement(9566..11425)
                     /locus_tag="NK788_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCH6768500.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11543..12703)
                     /locus_tag="NK788_000121"
     CDS             complement(11543..12703)
                     /locus_tag="NK788_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCH6768501.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13010..14434)
                     /gene="araA"
                     /locus_tag="NK788_000122"
     CDS             complement(13010..14434)
                     /gene="araA"
                     /locus_tag="NK788_000122"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCH6768502.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14450..15148)
                     /locus_tag="NK788_000123"
     CDS             complement(14450..15148)
                     /locus_tag="NK788_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCH6768503.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15105..16766)
                     /locus_tag="NK788_000124"
     CDS             complement(15105..16766)
                     /locus_tag="NK788_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCH6768504.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16984..18384
                     /locus_tag="NK788_000125"
     CDS             16984..18384
                     /locus_tag="NK788_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCH6768505.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18636..19715)
                     /locus_tag="NK788_000126"
     CDS             complement(18636..19715)
                     /locus_tag="NK788_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6768506.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19801..20760)
                     /locus_tag="NK788_000127"
     CDS             complement(19801..20760)
                     /locus_tag="NK788_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCH6768507.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20772..22283)
                     /locus_tag="NK788_000128"
     CDS             complement(20772..22283)
                     /locus_tag="NK788_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCH6768508.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22305..22868)
                     /locus_tag="NK788_000129"
     CDS             complement(22305..22868)
                     /locus_tag="NK788_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCH6768509.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22874..23737)
                     /locus_tag="NK788_000130"
     CDS             complement(22874..23737)
                     /locus_tag="NK788_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6768510.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23751..24647)
                     /locus_tag="NK788_000131"
     CDS             complement(23751..24647)
                     /locus_tag="NK788_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6768511.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24822..26111)
                     /locus_tag="NK788_000132"
     CDS             complement(24822..26111)
                     /locus_tag="NK788_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6768512.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26083..27600)
                     /locus_tag="NK788_000133"
     CDS             complement(26083..27600)
                     /locus_tag="NK788_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCH6768513.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27798..28688
                     /locus_tag="NK788_000134"
     CDS             27798..28688
                     /locus_tag="NK788_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCH6768514.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28761..29585)
                     /gene="nadE"
                     /locus_tag="NK788_000135"
     CDS             complement(28761..29585)
                     /gene="nadE"
                     /locus_tag="NK788_000135"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCH6768515.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29597..31069)
                     /locus_tag="NK788_000136"
     CDS             complement(29597..31069)
                     /locus_tag="NK788_000136"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCH6768516.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31706..32068)
                     /locus_tag="NK788_000137"
     CDS             complement(31706..32068)
                     /locus_tag="NK788_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCH6768517.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32291..33043
                     /locus_tag="NK788_000138"
     CDS             32291..33043
                     /locus_tag="NK788_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6768518.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33183..34106)
                     /locus_tag="NK788_000139"
     CDS             complement(33183..34106)
                     /locus_tag="NK788_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6768519.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34121..34393)
                     /locus_tag="NK788_000140"
     CDS             complement(34121..34393)
                     /locus_tag="NK788_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768520.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10179 a         7196 c         5947 g        11185 t
ORIGIN      
        1 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtaaa
       61 atcagcggtt tgacctgagt attagagata caaaacggaa aaatagcttc tcatattttg
      121 ccgttttttg agctaatacc gaaggacagc tgattgaaca ccgtttattc ggaaaaaggg
      181 actgtgacac gacttttgtc acagccccta aaaaaaataa taaatttgta ctattctatg
      241 aagcttcttc ttcagaaaac tgactgttat aaagatctgc atagaatccg tctttttcca
      301 tcaatgtatc gtggtttcct gtttctacga tcgaaccatg attcatcaca ataatgttgt
      361 ctgcatcgcg tattgtagac aagcggtgag caacaacgaa acttgtccgg ttttccaata
      421 ggcggttcat cgctttttga atcagtattt ctgttcgtgt atctacacta gatgttgctt
      481 catcaaggat caggacatct ggatttgcta agaatgcccg cgcaattgtg attagctgac
      541 gctgcccttg cgaaatattg cttgcatcct cgtttaatac agtgttatat ccttctggca
      601 atttacgaac aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt tgttcgtctg
      661 ttgcatcgtc gttcccatat ttgatattgt catagatact tcctgtaaac agccaagtat
      721 cttgcagtac cattgaaaac tgtgcgcgca attcatcacg tgaaagatca cgggtatcta
      781 caccatcgta acgaatacta cctccagaaa catcgtagaa ccgttcaaga aggttgatca
      841 gtgtcgtctt ccctgcacca gtcggaccta caatagcgac catctcacca ggtttgacat
      901 ccaagttaaa atcagtcatt agcaagttat ctggtgtgta gccgaattga acattttcaa
      961 aacgaacttt gtatggtgta tctttttctt ctggaaggcc agatttttca ttggacatct
     1021 cttcttcatc caacacttca aatacccgtt cggctgaagc aactgttgat tggatagtgt
     1081 tcatcaaact tgcaagttga gtgattggtt gagagaactg tgttgtatat tgcaagaacg
     1141 cttgaacatc ccctaatgtc atgttcccat ttgcaacttt tactccgcct agtacagcga
     1201 cgaacacata gcctagattt ttaacgaaat tcatcaacgg catgatgatt gccgaaataa
     1261 attgagcttt ccaaccagcg gcatacagat tctgattttc tttttcaaat acttcctggt
     1321 ctttttcagt atgattgaat gttttaacaa tggtatgacc accatatgtt tcttctactt
     1381 ggttattcaa taaacctaaa cttttttgct gatctgcgaa aaacacttgt gatttcggcg
     1441 ctacgatcat cacgatgatc aagcttaaag ggatcatcaa taacgcgact aacgtcaatt
     1501 gccagctgat tgaaagcatc atcacgatga ccccaatcaa tgtgactgca ctagttacaa
     1561 attgagtcaa attttgctgc agcgtgcttg cgatgttatc catatcattg attgctcgag
     1621 acatgatatc cccatttgta tgtgtatcat aataggaaat cggtacacga ttcattttgg
     1681 cttccaaatc atgacgtaat tcataaactg ttcgttggga aacacgtgtc ataataaatt
     1741 gctgtaaaaa gttgaataca gcagaaatca aatacatcac gattactgtc gcaatgatct
     1801 gcgcgatttt atcaaagtct atcggaaatt ttgttacttg ttggccttgt tgcatctgtt
     1861 gagcgccagc cattacacct ttgaatattt ctgtggttgc tttccccaag acctttggtg
     1921 tttggatttg gaagatcacg gctgctattg ctagtacaaa tacagcgatg atcgcaacca
     1981 tacgtttaga catatagcca aacaatcggc gtaccgttcc ccagaaattt ttaggctttt
     2041 caccttttac accgagattt ctgccaggtc caccatgtcc tggaccgcct gctggtccat
     2101 gagagggacg tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt gtgaatgaac
     2161 gatttcttga taagtttcgt ttgttgctaa tagttcttca tgtgttcctt tgccaaccag
     2221 ttttccttca tctagcacaa ggatcatatc tgcatccatt actgtactta ctcgttgagc
     2281 aactaaaacg gtgatacttt ctttcatatt cgttttcaat gcttggcgaa gattggcatc
     2341 cgttttgaaa tccaacgctg agaaggaatc atcaaacaca tagatatctg ctgttttgac
     2401 taatgcccgt gcaattgcca gacgctgtct ttgtccacca gagaagtttc ctcctccttg
     2461 ttccacatgg ctatctaagc catcttccat ctctgaaaca aaatctttag cttgtgcgat
     2521 ttctaatgct ttccaaatca tttcatctgt tgcatccggc ttaccatact gcatattgtc
     2581 tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca aagcccatta attctcgcaa
     2641 attatattga gtcatgtcac taatgtccgt tccatttatt tggatcgaac cagattcgat
     2701 gtcatataat cgagggatca aattgactaa ggtcgtttta cctgaaccag tcccccctat
     2761 gattgcgacg atctcgcccg acttggcttg gaaatcaata tcttccaatg caagtttttc
     2821 tgctccatga tatcgataat tgacatgatt gaaggccaat gttgcgtttt ttccagcaaa
     2881 agaaactgtt ttaggatttt cagggtcatg gataccgatt ttttcatcca acacttcatt
     2941 aatacggtca gctgaagctt gtgcacgagg caccatgata aaaatagcgg aaagcatcat
     3001 aaagctgatc aagatctgca tcgcataagt cataaatgca atcagattac cgacttccaa
     3061 agtcatatct gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg taccgctcat
     3121 gatcaatgtc atcattggca tcatcaatgc gacgatcgta ttaactttga ttgccgtatt
     3181 tgtataatct ttattcgcta aatcaaaacg attttcttca aagcgtgtct tatcgaatgc
     3241 tcgaatcact ctgacccctg tcaatccttc tcgaaatacc aggtttaaac ggtcagtttt
     3301 cttttgcata cttttgaata atggaacagc aaagtacatg attccgccga tcaaaataat
     3361 cattactggg atgacaacta agaagatttt tgtcaactga tgatcttttt gataagcaag
     3421 tatgctcgcg ccaatcaacg tgattggtgc attgatcatc atacgtaaaa acatttgtgt
     3481 aaccatttgg atttgagtca catcattggt cgttcttgtg atcaaagagg ccgttccata
     3541 tttatcaaac gcatcttttg tcagactctc tgatttttta tagacatccg tacgcaattg
     3601 cttacccagt ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag cagctaagat
     3661 actgatcaaa gaaaagccga tcatcacaaa acctgtatgc caaatataat cgacatctcc
     3721 ttgcgctacc cctttatcaa taatattgga ggtaagtgtc ggcaaatata aatctgccag
     3781 tacttggatc accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga tccgttttac
     3841 gatttttagc atctagttcc cttctttcta tttatatact ccgtttttca gccaagaaag
     3901 tttcaaattg aattcactga ttgcttgctc tactgagtaa tcttcagatg ttgaaaataa
     3961 gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc actaattgga tcagtaattc
     4021 caattcatga tcatttttta ctttcagctt cgtccgatcg atcaattcaa gcaactcttt
     4081 tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac tcgtatgagg
     4141 agcgactttt cttgaggcac ggtaatccat gttcataaac aagttacgat aaaaagaagc
     4201 attctcgcct tccaaaatct cataaatcat tttagaaaaa taggattcga atcctttgaa
     4261 tagatcacca tcggattctt tcattagttg taacatatct ttagagctgt cacgtctcat
     4321 tgtctcaaag taataaaaat aaagatcctc tttatcttca aaatactgat agaaactgcc
     4381 tctagggata tccgctaatt tgacgatatt agcaatcgaa gcttctttta agggtactct
     4441 agaaaactcg attctcgcag cctcgattag tcttttttgt ttttctttag gcaaatggaa
     4501 aaatgtctgt gtcggcatat ggatcctcct ttctttcaag atgacacatt gtcatatgac
     4561 gctgtgtcat tataacacct cctacctgtt ttgcaagaca tttgcatagt ttttttcatt
     4621 atcatccgat acattaaaaa agtgataaga ggaatccttc catttgaatt aatgagagtt
     4681 gcccattaaa atagccacat actaaacaaa ggatgagata aatggcccaa tcaattagtg
     4741 tccaagactt caataaattg cctctcgatg ctgacagcat cgttttagat acaagaaata
     4801 ccttagatta cacaaaaaac cacttaagcc actcgctttc tatccccgct gctatcttgc
     4861 caagacaatt aaaaaatcta gatccaaaga aactttatta tattctaagc tatacaggca
     4921 ggcgatcaga agcattagcc gatcaactta ctgctaatgg ttttcgtgct gtttatatca
     4981 ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt agattcaaaa
     5041 gaggctgtga caaaaaatcg tgtcacagcc tcttttatac cctgtctttt tttagttatt
     5101 tattcttcaa ccttatcaat atgacttgat agccttttct ttttctcata gttataacaa
     5161 tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc aaaggtcatc
     5221 ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta acaagttata
     5281 aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat gatattccgg
     5341 aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca gaattcactt
     5401 ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact gatcagttcc
     5461 ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc ccaacaaaat
     5521 taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat acagaaaacg
     5581 ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat ggcggtgtat
     5641 acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt gtaggcatcg
     5701 atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt gcagatgagt
     5761 tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa aaattgagat
     5821 aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgta ccttggatat
     5881 tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt cttcataaag
     5941 agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag ctgctgcata
     6001 aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag tcagcagaac
     6061 taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg gagagatcaa
     6121 gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga tcatgaatgg
     6181 acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc ctaagtaagg
     6241 gatcaggttc aaaaaacccg caataatccc caacgtgaca gcatactcta aaccaatgac
     6301 cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt gtcctcttat
     6361 ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt tttgcatgcg
     6421 tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca atagataaaa
     6481 taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc cgacaaaact
     6541 tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg ccgaattcat
     6601 gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa
     6661 aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac tgatgaagct
     6721 cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga ttagtgcaac
     6781 aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct tttcaaagta
     6841 atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag gcagacctac
     6901 gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac
     6961 gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat ttaaaaacca
     7021 gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa ttcctcctgt
     7081 tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg atgaagatag
     7141 attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga tacgtgagct
     7201 actgtctcta aattcccatt ggccatcgga ccgacataat cgatcgtata tacctgatca
     7261 tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga
     7321 atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat cagcatgggt
     7381 tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat cattgcactc
     7441 tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc cgtgtggaat
     7501 aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga atcagctcat
     7561 atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg aatctatacg
     7621 atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt tgaagaaaat
     7681 agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac tagtgtggat
     7741 ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc ggtaacagag
     7801 tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact atacggggcg
     7861 aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga actgaaagca
     7921 gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat
     7981 gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct tggtacagat
     8041 cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac taccttcact
     8101 gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca attcgcttat
     8161 ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga aacaggtgct
     8221 tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga aaatggaggg
     8281 gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg aggacataac
     8341 tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc
     8401 tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa ttttgtcgtg
     8461 tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga aacaggatta
     8521 ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt
     8581 tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact acgggaagaa
     8641 ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga agaggaatat
     8701 cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa tgtagaaaac
     8761 taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc gtctatcctg
     8821 atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga aaaggagtat
     8881 ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca ttatcataaa
     8941 ggattttgat cactctatca taaaggtaat attacattta tattagataa ctctagtttt
     9001 tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt tttggatggc
     9061 agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga atagatcttc
     9121 atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg ctacatatga
     9181 aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg gacgcaaagt
     9241 actgactttc ttaagcattg cccgatattt ttttgttcct tcttcaaata acaggatgat
     9301 ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg cgtttctgat
     9361 cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga cgatttgatc
     9421 gatggactgc acataatagc caatattcgg taacatgttc gttttcctac tttctccatt
     9481 gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg aaaaaattta
     9541 atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct gctaaactat
     9601 attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa atctcttgtt
     9661 gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac agttctcgtt
     9721 caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca tttgttgtct
     9781 gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag
     9841 tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt tcatgcaatg
     9901 tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga tgatcccccc
     9961 aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg
    10021 ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc ccacttccat
    10081 ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc atatgtgtct
    10141 gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt gtcattactt
    10201 ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc gtataggtca
    10261 tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc tctcttttat
    10321 acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg tttaacgctg
    10381 aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt
    10441 gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata tttgctgttc
    10501 caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct acttcataat
    10561 aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga tctgagaaac
    10621 tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta tttttttcat
    10681 cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa tatccttcag
    10741 gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa ccagtgttat
    10801 agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat gcttttcgca
    10861 acaaattatc gggattgttg aaattactga tataccctag cagtccaatt gccacaagca
    10921 gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga aacatgtacc
    10981 agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac agcatagtgc
    11041 cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct ggataaatcg
    11101 gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg atggctacga
    11161 catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa ataagaataa
    11221 tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca
    11281 acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta ctaaaaacaa
    11341 caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc tgtttggtcg
    11401 aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa taaaagaatg
    11461 ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat
    11521 gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag cggacctttc
    11581 gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca aaatcagtac
    11641 tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat atatctgcga
    11701 tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg gttttctttt
    11761 gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat ggataattga
    11821 ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact gccaaacgtc
    11881 cgctgtctgc caaagcttcc tgagtcgatc cccctagatg aggcaacata agtatacggt
    11941 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
    12001 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
    12061 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
    12121 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
    12181 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
    12241 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
    12301 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
    12361 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
    12421 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
    12481 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
    12541 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
    12601 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
    12661 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
    12721 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
    12781 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
    12841 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
    12901 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
    12961 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
    13021 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
    13081 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
    13141 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
    13201 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
    13261 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
    13321 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
    13381 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
    13441 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
    13501 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
    13561 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
    13621 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
    13681 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
    13741 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
    13801 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
    13861 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
    13921 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
    13981 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
    14041 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
    14101 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
    14161 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
    14221 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
    14281 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
    14341 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
    14401 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
    14461 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
    14521 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
    14581 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
    14641 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
    14701 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
    14761 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
    14821 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
    14881 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
    14941 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
    15001 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
    15061 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
    15121 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
    15181 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
    15241 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
    15301 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
    15361 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
    15421 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
    15481 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
    15541 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
    15601 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
    15661 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
    15721 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
    15781 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
    15841 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
    15901 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc gtttggctac actgttcgta
    15961 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
    16021 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
    16081 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
    16141 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
    16201 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
    16261 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
    16321 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
    16381 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
    16441 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
    16501 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
    16561 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
    16621 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
    16681 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
    16741 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
    16801 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
    16861 aagcggttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
    16921 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
    16981 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
    17041 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
    17101 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
    17161 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
    17221 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
    17281 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
    17341 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
    17401 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
    17461 tatttgctaa aagtgttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
    17521 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
    17581 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
    17641 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
    17701 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
    17761 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
    17821 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
    17881 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
    17941 actttattga aaataggcgg tagcgtgatg ggactttcat tcatcctacc agctgtttta
    18001 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
    18061 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcatagg cgaaatattc
    18121 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
    18181 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
    18241 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
    18301 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
    18361 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
    18421 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    18481 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
    18541 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
    18601 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
    18661 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
    18721 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
    18781 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
    18841 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    18901 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    18961 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    19021 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    19081 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    19141 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    19201 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    19261 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    19321 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    19381 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    19441 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    19501 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    19561 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    19621 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    19681 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    19741 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    19801 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    19861 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    19921 tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt
    19981 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    20041 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    20101 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    20161 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    20221 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    20281 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    20341 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    20401 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    20461 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    20521 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    20581 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    20641 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    20701 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    20761 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    20821 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    20881 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    20941 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    21001 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    21061 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    21121 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    21181 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    21241 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    21301 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    21361 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    21421 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    21481 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    21541 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    21601 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    21661 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    21721 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    21781 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    21841 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    21901 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    21961 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    22021 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    22081 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    22141 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    22201 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    22261 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    22321 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    22381 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    22441 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    22501 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    22561 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    22621 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    22681 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    22741 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    22801 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaaattt agtctcaact
    22861 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    22921 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    22981 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    23041 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    23101 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    23161 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    23221 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    23281 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    23341 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    23401 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    23461 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    23521 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    23581 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    23641 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    23701 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    23761 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    23821 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    23881 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    23941 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    24001 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    24061 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    24121 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    24181 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    24241 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    24301 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    24361 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    24421 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    24481 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    24541 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    24601 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    24661 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    24721 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    24781 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    24841 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    24901 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    24961 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    25021 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    25081 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    25141 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    25201 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    25261 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    25321 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    25381 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    25441 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    25501 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    25561 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    25621 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    25681 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    25741 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    25801 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    25861 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    25921 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    25981 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    26041 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    26101 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    26161 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    26221 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    26281 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    26341 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    26401 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    26461 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    26521 tgtttctgtc ctgattggtg caataacgtt taccaattga gcaagacaag caatctttac
    26581 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    26641 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    26701 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    26761 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    26821 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    26881 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    26941 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    27001 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    27061 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    27121 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    27181 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    27241 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    27301 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    27361 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    27421 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    27481 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    27541 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    27601 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat
    27661 ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat taataatgat
    27721 taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct
    27781 tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag
    27841 cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg
    27901 tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa
    27961 agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc
    28021 ttcgtgtcga taaaatcgat attagttttc cagaaaaaat attcaatgct tttgatacga
    28081 aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt
    28141 tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc
    28201 gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta
    28261 attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg
    28321 aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg
    28381 agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg
    28441 attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac
    28501 acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag
    28561 agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct
    28621 gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact
    28681 acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga
    28741 cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt
    28801 cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact
    28861 tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct
    28921 gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc
    28981 ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc
    29041 aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca
    29101 tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata
    29161 cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt
    29221 aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc
    29281 tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga
    29341 cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt
    29401 agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa
    29461 gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt
    29521 tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg
    29581 ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt
    29641 ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg
    29701 gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt
    29761 tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta
    29821 aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga
    29881 acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt
    29941 cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta
    30001 ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga
    30061 tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg
    30121 tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt
    30181 catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt
    30241 cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt
    30301 tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg
    30361 tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg
    30421 cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta
    30481 tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg
    30541 ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata
    30601 gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga
    30661 aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca
    30721 cttggatcaa tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg
    30781 ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac
    30841 gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt
    30901 ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa
    30961 aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat
    31021 atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact
    31081 cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa
    31141 tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc
    31201 catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt
    31261 ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa
    31321 ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata
    31381 accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc
    31441 cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg
    31501 gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac
    31561 acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg
    31621 cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta
    31681 ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca
    31741 ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca
    31801 ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt
    31861 cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat
    31921 atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag
    31981 ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac
    32041 tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga
    32101 tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc
    32161 tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt tcatgagaaa
    32221 aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg
    32281 aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag
    32341 aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg
    32401 ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac
    32461 aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca
    32521 gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct
    32581 cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa
    32641 agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca
    32701 agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa
    32761 tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct
    32821 cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca
    32881 taagaagtgg ttatcttaca gctggcctga atatcagcca aaagcgcata aaggactagg
    32941 aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg
    33001 tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa
    33061 caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt
    33121 atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat
    33181 attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac
    33241 aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga
    33301 agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag
    33361 ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc
    33421 tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca
    33481 agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta
    33541 taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc
    33601 aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt
    33661 tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca
    33721 tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct
    33781 tcaagatacc ttcgctcttt ttctttctga tagcccgtcg gtaaaagtgt cactttgtaa
    33841 tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg
    33901 gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt
    33961 gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca
    34021 tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc
    34081 tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt
    34141 gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg
    34201 aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca
    34261 ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg
    34321 atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta
    34381 cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc
    34441 tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa
    34501 gtctctt
//
LOCUS       DAHUWM010000005        33864 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHUWM010000005 DAHUWM010000000
VERSION     DAHUWM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.005"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            157..2055
                     /locus_tag="NK788_000141"
     CDS             157..2055
                     /locus_tag="NK788_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCH6768521.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2354..3073)
                     /locus_tag="NK788_000142"
     CDS             complement(2354..3073)
                     /locus_tag="NK788_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCH6768522.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3410..3742
                     /locus_tag="NK788_000143"
     CDS             3410..3742
                     /locus_tag="NK788_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768523.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3729..5732
                     /locus_tag="NK788_000144"
     CDS             3729..5732
                     /locus_tag="NK788_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCH6768524.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5751..6221
                     /locus_tag="NK788_000145"
     CDS             5751..6221
                     /locus_tag="NK788_000145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCH6768525.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6242..6826
                     /locus_tag="NK788_000146"
     CDS             6242..6826
                     /locus_tag="NK788_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCH6768526.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6841..7827
                     /locus_tag="NK788_000147"
     CDS             6841..7827
                     /locus_tag="NK788_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCH6768527.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7817..8128
                     /locus_tag="NK788_000148"
     CDS             7817..8128
                     /locus_tag="NK788_000148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCH6768528.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8150..9931
                     /locus_tag="NK788_000149"
     CDS             8150..9931
                     /locus_tag="NK788_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCH6768529.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9924..11300
                     /locus_tag="NK788_000150"
     CDS             9924..11300
                     /locus_tag="NK788_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCH6768530.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11307..11942
                     /locus_tag="NK788_000151"
     CDS             11307..11942
                     /locus_tag="NK788_000151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCH6768531.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11972..13327
                     /locus_tag="NK788_000152"
     CDS             11972..13327
                     /locus_tag="NK788_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCH6768532.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13519..14913)
                     /locus_tag="NK788_000153"
     CDS             complement(13519..14913)
                     /locus_tag="NK788_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCH6768533.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            15005..15976
                     /locus_tag="NK788_000154"
     CDS             15005..15976
                     /locus_tag="NK788_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCH6768534.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15973..16755
                     /locus_tag="NK788_000155"
     CDS             15973..16755
                     /locus_tag="NK788_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCH6768535.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16924..17295)
                     /locus_tag="NK788_000156"
     CDS             complement(16924..17295)
                     /locus_tag="NK788_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6768536.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17429..17872
                     /locus_tag="NK788_000157"
     CDS             17429..17872
                     /locus_tag="NK788_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCH6768537.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17915..18724)
                     /gene="yidA"
                     /locus_tag="NK788_000158"
     CDS             complement(17915..18724)
                     /gene="yidA"
                     /locus_tag="NK788_000158"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCH6768538.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18743..20110)
                     /locus_tag="NK788_000159"
     CDS             complement(18743..20110)
                     /locus_tag="NK788_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6768539.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20094..20924)
                     /locus_tag="NK788_000160"
     CDS             complement(20094..20924)
                     /locus_tag="NK788_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCH6768540.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21087..21524
                     /locus_tag="NK788_000161"
     CDS             21087..21524
                     /locus_tag="NK788_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCH6768541.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21600..22220
                     /locus_tag="NK788_000162"
     CDS             21600..22220
                     /locus_tag="NK788_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCH6768542.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22409..22915
                     /locus_tag="NK788_000163"
     CDS             22409..22915
                     /locus_tag="NK788_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCH6768543.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22932..23831
                     /locus_tag="NK788_000164"
     CDS             22932..23831
                     /locus_tag="NK788_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCH6768544.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23918..24802
                     /locus_tag="NK788_000165"
     CDS             23918..24802
                     /locus_tag="NK788_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCH6768545.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24994..25065)
                     /locus_tag="NK788_000166"
     tRNA            complement(24994..25065)
                     /locus_tag="NK788_000166"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25030..25032),aa:Arg,seq:ccg)
     gene            25125..26459
                     /gene="rpoN"
                     /locus_tag="NK788_000167"
     CDS             25125..26459
                     /gene="rpoN"
                     /locus_tag="NK788_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCH6768546.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QERLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26608..27699
                     /locus_tag="NK788_000168"
     CDS             26608..27699
                     /locus_tag="NK788_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCH6768547.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27740..28741
                     /gene="gap"
                     /locus_tag="NK788_000169"
     CDS             27740..28741
                     /gene="gap"
                     /locus_tag="NK788_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCH6768548.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28851..30041
                     /locus_tag="NK788_000170"
     CDS             28851..30041
                     /locus_tag="NK788_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCH6768549.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30145..30900
                     /locus_tag="NK788_000171"
     CDS             30145..30900
                     /locus_tag="NK788_000171"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCH6768550.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            31016..32314
                     /gene="eno"
                     /locus_tag="NK788_000172"
     CDS             31016..32314
                     /gene="eno"
                     /locus_tag="NK788_000172"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCH6768551.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            32713..33852
                     /locus_tag="NK788_000173"
     CDS             32713..33852
                     /locus_tag="NK788_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCH6768552.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        11186 a         5997 c         7210 g         9471 t
ORIGIN      
        1 gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg tatagaccta
       61 tttctatttt tttattatat tggatatata tattcattga aaatagagca taacaacttt
      121 ataatgttga gtataccaat ttttggaggc gttttaatgg accgctattt agatttactt
      181 tcagagaagt tccctacaac ggaagatgtc atcacggaaa tcatcaatct cgaagcaatt
      241 ttgcagcttc caaaaggaac agagttattt ttgagcgata tacatggcga atttcctgct
      301 tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttc
      361 tctgaacaaa tgacggaaac ggagatcagc caattcacta tttttacggc ttatccagaa
      421 tatgtgttga cgactgattg gtacaaaaaa caagataaat cccagctgat ccatcgtttg
      481 atcgatcttt tacggtttac gacagtcaaa tacactcgat caaaagttcg aaaaggattg
      541 ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgatcg tatcaacggg
      601 aaaaaagact atgtaaaaaa aatcattgaa caacttgtct tgatgaagga agaagaaaaa
      661 tttttaacaa aacttgcgca gacgatccaa aaatctgtca tcgatcattt gcatatcgtg
      721 ggtgatattt ttgatcgagg aactcaagca gccaaagtaa tggatcgaat catggatttt
      781 tcctctgtag atatccagtg ggggaatcat gatattttgt ggattggtgc gtattgcggc
      841 tctgaggcat gtttattgac gcttttacgg attgccgcac gctacaacta tttatttgaa
      901 ttagagaaag aatatggatt aaatttacgt ccattatttt cttttgccca tcaaacctat
      961 cagaagaatc cagtatttac ccctaaaaac aggaaagatc tatctgaaag agagcaagaa
     1021 gaattagaaa agatccatca agcactttct atcctccaat tcaaatcaga acatcagttg
     1081 ttgaatcgac gacctgaatt taaaatggac gatcgtaaat tgttggaaaa aatcaactat
     1141 gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt tgaaagggac ttgttttcaa
     1201 acgatccagc cagatgatcc taaaaaattc ttggaagagg aacaaaaagt gatcgatagt
     1261 ttgatgtatt cattccagca ctcgttaaga atgcaaaaac acatgacgtt tttgatcgaa
     1321 aaagggggaa tgtatctcac gaacaatcag catgtactgt tccatggctg tatacctgta
     1381 gatgaaaagg ggcagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
     1441 ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag atttgactgc aaatgaagat
     1501 ttggcgaccg atttgatttg gtatgcgtgg agcgggccat tttcgccttt attcggcaag
     1561 agtaaaatgg caacttttga gcgctatttt ctcacagagc ctcatacgca cgtcgaaaaa
     1621 agcaatccct attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttggcagaa
     1681 ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac acacgccagt aaaagtaaaa
     1741 aaaggagagt caccgatcaa agcaaacgga aaagcatttg ttattgacgg aggtctatcc
     1801 aaagcttatc aaaaaacaac cgggattgct ggctactcat tactatgcaa ttcttacggt
     1861 ttccaaatcg tgacacatta tccgttcttg cctattgaac aacaatttgc aaaaaaatct
     1921 gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc cacgcaaact taatcggaat
     1981 acgacaaaag gaatggaatt acagcaacag attactcaac tccaaaggct gttggattat
     2041 cgaaaggacg attgagaaaa atgctagcca acattgctca atagactaaa aagcgactgc
     2101 aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa aaaacggtaa aatattagaa
     2161 gctatttttc tgtttatttc tctaatactc aggtcagggc gctgatttca cgacctcttc
     2221 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca acaaacatga
     2281 gaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgaccta
     2341 atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa taaacgctgt tcagatgaat
     2401 ctacacgatg agaaagttcc ggatgccatt ggacaccaag gattttgtgc atattatctt
     2461 tactttggac tgcttcaatg agtccatcgt tggaaaaggc gatggctttt aacaaaggag
     2521 aaagatcttt gactgcctga tgatgatagg aattcacaaa gtatgaatca gggagaaggt
     2581 cacttaataa actgtccgaa acgattttga cttcgtgagt tgcaaactgc ggggcagtgg
     2641 gctgttgttc gtgttttacg gaccattttg gatataatga aagatcttga taaagtgttc
     2701 cctccaacac gacattcaat agttgaagtc ctcggcagac tgcaaaaatc ggttttcttt
     2761 gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc tcgttgaata tttgtttccg
     2821 tcagttttgg atgagggtct tccatgtaaa gttgcggact aacatcctga ccgcctgcaa
     2881 gaatgagttt gtcgattttc tcaattagtt gaggcgctga atctggagac atgactggca
     2941 gaataagagg taatccttga gcatctaaaa ctgcatccac aaatccctgc ggtgtatagc
     3001 ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag ttgattcccg gcaatgccga
     3061 tgattggttt catttttatt ccccctaaag tagactaatt gtaacaagga tttaaaaaaa
     3121 tttaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta ctatctgtaa
     3181 agagaatttt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat ttgttataat
     3241 aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt tcttcaaggg
     3301 gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta gaggtacata
     3361 ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag tgaagcaagt
     3421 gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgagaagc gtgctcgaac
     3481 cctacggcag cagttgaaga cctatgcaga agaaaaagag caagaattgg agataatcca
     3541 aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa aaagaacaat tcgaaaagca
     3601 agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc aagcaacttt
     3661 gaaagaccaa tatcaaaagc atcagagtca agcaatcggt gcaatcattg aaagggtgaa
     3721 agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat aaaagaaatc
     3781 aggagaggat tttacaagca gtacaaggaa tccaaaatgt tgaaatccgg gatctgtatc
     3841 aaaatagaac aaacaaccag tgggtagaaa aatattttcc agataccacg attattgata
     3901 aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga catccaagaa gctatccaat
     3961 tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt caagaacttt
     4021 ccttacaaga cttggaagca aattattccg aagaaaattt catgaataaa ttagaagaag
     4081 tccttgattt aaaagaaaag tgggaacagc taactgaaaa acgtgatcgt ttagaagaag
     4141 aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg tatgcaagta
     4201 agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag
     4261 aactaagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca aaacaagttt
     4321 atctttcgta tgttgcttta aaaaaatcag ttgctgctat agaagaaatt gctgggaaat
     4381 acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa ttgcaagaaa
     4441 ccaaacagca attatccatt gttcacgagc agcaaaaaaa gataacgaca gcgttaggtc
     4501 agtgcagcgg ctatatccaa gaatttgaat gggtagaaga agtcacactt gccgttgcag
     4561 aacgggaaaa agtaaaagaa cggtttatcc atgccactta tctgatcgtt ttgcaaggtt
     4621 gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt aaacgaaact gtatcagaag
     4681 aagatatata cgttagtttc gaaacaccaa actcagaaga gattcagcag gaagtcccta
     4741 ctaaattgaa aaaccatccg cttatcgaac cttttgaatt gctgactgaa atgtacagtc
     4801 tgccaaaata tgaagaagta gatccaacgc cttggatgac accattttat cttgtcttct
     4861 tcgggatgat ggtcgcagat gttggttacg gattgctgat gctcattggg acaatactag
     4921 ctcaaaaact atttgtttta ccccgcggga tgaaacgttt cgtaaaattc tttgagattc
     4981 tctcggtacc atcgatcatc tggggattga tctacagttc atttttcgga caggcattac
     5041 caaaagaact atttggtatc cgcttgcctt tcccaatcct atctacgacg gatgacgtca
     5101 atacgatttt gattttatcc gtgattttcg gtctgataca gatcttggtt ggattattcg
     5161 tggcagcaaa ggaaaatatc aggaggaaag attacttgag tgctgtcagt gatggttttg
     5221 cctggcaagg gatcctgatt gggatcgtta ttgcattgat tggtgtcatg ttgataaaaa
     5281 atacagcatt tgtttatctt ggcgcggggc tatcgatcat tgctgcttta tgtatcttaa
     5341 tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc tgccaaagga gcgtataatc
     5401 tttacggatt gactggttat atcggtgatt tagtcagtta tacacgtttg atggcactag
     5461 gtatttcagg cggaagtatt ggcgcggcat tcaatatgct agttgctttc atgccgccag
     5521 ccgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg aatatgtttt
     5581 tgacactgtt aagtgcctat gtccatggtg cacgattgca gtatgtcgaa ttttttggaa
     5641 aattttatac aggaggcgga cgtgcctttg acccgctgaa aacggcggaa aaatacgtca
     5701 acatcaatca taagaaaaaa aacaataaat aattggagga tttttataca atgatggatt
     5761 acttaattag tcaaaatggt ggaatggtat ttgcagtatt agcgatggca acagcgacga
     5821 ttttttcagg gattggttca gctaaaggtg ttggaatgac aggtgaggca gcagcagcgt
     5881 taacaacaag tcaacccgaa aaattcggac aggctttgat tctgcaattg cttccaggta
     5941 cgcaagggtt gtatggattc gttatcgcct ttttgatttt tatcaactta ggaaatgata
     6001 tgtcagttgt acaaggattg aactttctag gcgcttctct gccgattgct tttacaggtt
     6061 tgttttcagg tatcgcccaa gggaaagttg cagcagcagg tatccaaatt ttagcgaaaa
     6121 aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca tatgcgattt
     6181 taggtttcgt tatttccttc ctattagtat tgaatgccta agcataaagg aggaaaagca
     6241 gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa gcagcagagc aagaaagagc
     6301 ggcgttagag caagaagagc gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca
     6361 aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattacttg aaaaaaaata
     6421 ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa gaaaatttga atgcgaagca
     6481 ggaattttta cgacgattgt ttgcagatgc aatagctgaa atggagaatt gggatgaatc
     6541 tgaacaaatc cagtttatca aaaattcatt gtattcatta cctttgacag gcaaagtaac
     6601 attcattgct ggagaaaaat cagcagctta tctttctcag acattgctgg atgaatggaa
     6661 caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc aagcgggttt
     6721 tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg tacaagatat
     6781 ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag ggatcagtgt
     6841 atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact tttgtccaat
     6901 gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga aatattgaaa
     6961 tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga acagaatctt
     7021 ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc
     7081 gtttggatct atacgatgcg ctatactttc cataatctga aggttttgac aaaagccgag
     7141 atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc attagaaacc
     7201 ttgaaggatg ccattcatac gcaagtttca aatgaactcc ctgaaagcat tatggacagt
     7261 atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat tgacgtgatt
     7321 tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg atatcaagaa
     7381 ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat ggctcgagga
     7441 atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc gggaagtatc
     7501 gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac gaattttcta
     7561 ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa tcttgatctc
     7621 gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga agcacaaacc
     7681 caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt agaaagcaaa
     7741 aaccttcgtt tactggttat cggcaaaaga agccatttcg aaccagaaac aatcagaaag
     7801 cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag attctgtttt
     7861 gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca
     7921 ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag agcaatgtgc
     7981 agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg ctatcgtatt
     8041 gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa aaaacgtaga
     8101 aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt tgcaaattgg
     8161 aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg atgccagtat
     8221 ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg agatgcgtgg
     8281 cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag gcgaaccggt
     8341 tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt cacaaatgtt
     8401 tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta atttcttagg
     8461 gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg aaccaacagt
     8521 agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag aaacaaaagt
     8581 tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa
     8641 aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa
     8701 tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa
     8761 cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc cagtgacaaa
     8821 aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca
     8881 aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa
     8941 tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata caggagaatc
     9001 gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag cagctcgaga
     9061 agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg gttatgacgt
     9121 tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga tgagcggacg
     9181 tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac ggcttgcgga
     9241 atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg aaggaagtat
     9301 aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag tcacacaaaa
     9361 tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc aaaaaagaca
     9421 ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag tagggaagta
     9481 tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca tgcgtatttt
     9541 gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt ccctttcaga
     9601 taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt tgcagcaaaa
     9661 tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa tgttgaagat
     9721 gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga
     9781 gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat atatcccgga
     9841 aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga tgcaaacgat
     9901 cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg
     9961 gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc
    10021 gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa gataaagcaa
    10081 tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct gtacgatttt
    10141 taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt
    10201 tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg gatattaacg
    10261 gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa acaggtatct
    10321 ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta ttttctggct
    10381 ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg gtactcgatt
    10441 catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa gaagctgaat
    10501 ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg tttatgaatt
    10561 tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg actgctgcag
    10621 aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac atgaccaact
    10681 atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga agaagaggat
    10741 atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga agaatccgcg
    10801 gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac gataaaactc
    10861 atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg actagagagc
    10921 tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg cggttaaaag
    10981 ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg aatcagcttt
    11041 ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta ggtgaatctg
    11101 ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa aaagaatatg
    11161 tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta gggtgggaac
    11221 tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg ctggacaaat
    11281 atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg acccgcatgg
    11341 aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa ttattaaaag
    11401 ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac aaccagctgc
    11461 gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg gcaaatgctt
    11521 cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac gttgatttgt
    11581 ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt gaatatgatg
    11641 aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc gaactggatc
    11701 ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca gaaatcgaaa
    11761 aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc aatgcattag
    11821 aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg aagctggaag
    11881 aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt accactgact
    11941 aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac aaataaagaa
    12001 acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt tagttggcgg
    12061 cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt ttatcgatgc
    12121 gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga atacagcaga
    12181 gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg gcggtcttgg
    12241 ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca gtctcagcac
    12301 acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga tgaagctcat
    12361 gctttacatc ctaaaattgg cctttgttat ccaattgatt ggtacggttg ctttatcttt
    12421 tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt tccatgcaat
    12481 ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca atcaccagca
    12541 aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt taggcttcat
    12601 cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc attcaaaaat
    12661 cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct ttttcacaga
    12721 gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa acaccttttt
    12781 tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc atatgagcca
    12841 tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg ggtcgacggc
    12901 cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa
    12961 aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag tgttgcgggc
    13021 acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt
    13081 gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg tcttctctgc
    13141 gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa tagggaaatt
    13201 gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg tcttctctct
    13261 gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat
    13321 tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa aataaaataa
    13381 tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt gaggcactac
    13441 ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata agatttgatt
    13501 cctttattta tcaataggtt ataattcata tttttttatt ttttctttta cgatacttag
    13561 tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga atgcatctgg
    13621 ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag caagaaagtc
    13681 attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat agtaagggac
    13741 acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg ctctttttgt
    13801 aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga tttgagtgat
    13861 gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt gttctatctt
    13921 gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac aaataaattt
    13981 tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag gaggaatgat
    14041 cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa cccatagacc
    14101 ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt gaaaccctat
    14161 aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc tacattcctg
    14221 aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga tctctaaatg
    14281 atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg ctattctatc
    14341 acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac cggaaacaat
    14401 agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg ctgcagtagt
    14461 caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa gatttgggtt
    14521 attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa aagaactaac
    14581 cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga tggactcaaa
    14641 gacattggga gaaataaatg tgccacttcc aatattcgta tacagcaaac ctttctcaat
    14701 tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat cagctagctc
    14761 tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat cttgttctaa
    14821 ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg gtcgccaagt
    14881 taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa attgtatacc
    14941 atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga ggagggaatt
    15001 gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg aagaagcgat
    15061 tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg
    15121 attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga tgcctgctta
    15181 tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg ataatcatca
    15241 gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa acggactacc
    15301 caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg tatctgcgct
    15361 ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg gagctggtca
    15421 acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta cagtgtttgt
    15481 ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa aacagtatcc
    15541 taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag cggaaatcat
    15601 ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc
    15661 acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta catcgttgat
    15721 ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa gcggggacct
    15781 gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta cattaggcga
    15841 attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga ttttcgacgc
    15901 agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca aattagagaa
    15961 aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga tgaggtgttc
    16021 aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa
    16081 aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa aaaccaagaa
    16141 tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca cgcgacatta
    16201 gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat taaattttct
    16261 actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat ggattttcct
    16321 agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat tggggctaag
    16381 attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga aaaaacggtg
    16441 gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat aggagtcatc
    16501 gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca
    16561 atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa
    16621 agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa
    16681 tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt
    16741 acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcaacc
    16801 ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg
    16861 cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac
    16921 taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga
    16981 aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccggcag ctttatgttg
    17041 ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc cgtctcctag
    17101 atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat ccccaggaag
    17161 tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa
    17221 accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc gaacacatgt
    17281 gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata acaaaaagaa
    17341 gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa
    17401 aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg aaaaaaagca
    17461 atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag atcagcagat
    17521 tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga
    17581 agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga
    17641 aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta
    17701 ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac
    17761 tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg
    17821 tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc
    17881 gagtagatca gctttctctc agttttttta tttattacag tacatatttt ttgattgctt
    17941 cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag
    18001 aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct
    18061 cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg
    18121 caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt
    18181 tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa
    18241 tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag
    18301 gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga
    18361 tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac
    18421 ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt cagtccgtga cgggcgatga
    18481 ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat
    18541 cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc
    18601 ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg
    18661 ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag
    18721 acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc
    18781 tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag gacggatcaa
    18841 ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa
    18901 caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt
    18961 gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg tagctagaat
    19021 tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa gtgagttctt
    19081 ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca
    19141 aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt
    19201 aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa
    19261 gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact
    19321 cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa acaggatgga
    19381 agtacacttg cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc
    19441 cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa
    19501 gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc
    19561 gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt
    19621 ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca
    19681 cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga
    19741 tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata
    19801 aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat
    19861 ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgagtaa
    19921 aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc
    19981 gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc tggacatgga
    20041 tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag
    20101 gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg
    20161 ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt
    20221 gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg
    20281 ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca
    20341 tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccgcgt
    20401 ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga
    20461 cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa
    20521 taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat
    20581 aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgggggaaa
    20641 atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg
    20701 atcactggag cgtaacctgc atctttcagt gtttggatcc cgtcacttag aaaaggagtt
    20761 cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt
    20821 tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg
    20881 tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg
    20941 tataaattca tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa
    21001 ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag
    21061 ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca
    21121 aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag
    21181 ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga
    21241 cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc
    21301 gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct
    21361 atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt
    21421 tgaaagaccg tcccacagga gggaaggttc gaatcgatta tgatctgttc atgggaccgg
    21481 aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt
    21541 tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat
    21601 tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac
    21661 acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc
    21721 aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg
    21781 acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat
    21841 ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag
    21901 atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg
    21961 cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg
    22021 atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc
    22081 aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag
    22141 aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt
    22201 ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag
    22261 cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt gtatataaaa
    22321 atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca
    22381 tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga aaaaacggca
    22441 gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc aaaaaatgtt
    22501 ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga tgcaagatag
    22561 ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt ttagtgcatg
    22621 gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa aaaggacgcc
    22681 tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa cttccgttgc
    22741 agagctccat atgattttag ataaaataga cagtccggaa aaagagattc tgatcctcta
    22801 cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga atgaaaacac
    22861 agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag aatgaaggag
    22921 agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa acttataaag
    22981 aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt atcgaagagc
    23041 aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga gtatctttgg
    23101 ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga agctttgcag
    23161 aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc tttactgatc
    23221 gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag aaagaaaata
    23281 aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat tttgaaaaag
    23341 ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga gattatcaga
    23401 aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa acagcagcag
    23461 atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc cagctctcac
    23521 ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa gtcaaacggc
    23581 agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag caagatattc
    23641 aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt tatttgaaca
    23701 aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt tatatgggta
    23761 caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg gaagattatt
    23821 tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt aagtacaagt
    23881 aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt atttagttat
    23941 cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat
    24001 caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc
    24061 agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca
    24121 agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca gtacaaaaac
    24181 gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac
    24241 cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt
    24301 cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca
    24361 cgagattggc aattacacat acgatcatca gaatctggcc actctttctg cccagtcgat
    24421 gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca aaacccccac
    24481 ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca tccagcgacc
    24541 gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg atttgatttt
    24601 gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg atatctatcc
    24661 tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag ggtatcgcat
    24721 cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt atggcagaaa
    24781 cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca cattttaata
    24841 agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat acaggtacaa
    24901 caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat aaagaagcaa
    24961 ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc gaacccccgt
    25021 ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca ctgggataat
    25081 tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa tttgagcaaa
    25141 atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag ttgcaacagt
    25201 ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag aatcaagcaa
    25261 tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac ataaaagctt
    25321 cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct gatacaaaag
    25381 ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat acttttttga
    25441 ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg acgattagtt
    25501 tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat gcgttgactc
    25561 tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa cgtctcatgc
    25621 ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg gaagaatgtt
    25681 ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct gttgatctac
    25741 atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct ggccggattt
    25801 tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg gacgacgata
    25861 aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt caagagagtt
    25921 acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta aaagaacgga
    25981 aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca attttgcggg
    26041 ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa cgtccaatca
    26101 agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc acggtcagtc
    26161 gtgcagtcaa cgggaaatat ttggaaacag atttcggtgt atttgaatta aaaaaatttt
    26221 tcactacacg aattcctacg aatagtacag aacagacaga agacctttca gcagatacag
    26281 cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg ttgtcggacc
    26341 agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga acagtagcaa
    26401 aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat gataactaat
    26461 cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg aaaaacgaga
    26521 gcgaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg
    26581 aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt ggacgtaaag
    26641 tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc tgttgcacca
    26701 gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta ttggatgcag
    26761 ccgattgggc gtcgaagttt atcggaaagt atggggatca cagaacgagt cttacgaaca
    26821 gaaacagacc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg tatgacgctg
    26881 acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca gcttttaggt
    26941 acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg tatcgttgtt
    27001 tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa
    27061 gccttagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg aggaactact
    27121 atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca taacctgttt
    27181 gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc tgtcagtgca
    27241 agaatggctg ctaaagcaaa cggtaaccac cgcgcacttt atgttccgga acaattaagt
    27301 ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt aaacttgatt
    27361 agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc agcacgtcgt
    27421 aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc ggaatctttt
    27481 ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat cggcatagaa
    27541 ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa atcaaaagct
    27601 aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa
    27661 gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt ttttgatttt
    27721 cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg gacgtattgg
    27781 acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag caatcaacga
    27841 cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc aaggacgttt
    27901 caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag aagttaaagt
    27961 attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag atatcgttct
    28021 agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa cagctggtgc
    28081 taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg tttataacac
    28141 aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat gtactactaa
    28201 ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg aaggattaat
    28261 gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc atcctaaagg
    28321 tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc
    28381 taaagctatc ggtttagtaa ttcccgaatt gaacggtaaa ttagacggag ctgctcaacg
    28441 tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa aagaagtaac
    28501 tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg gatacaacac
    28561 agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat ttgatgctac
    28621 acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg cttggtacga
    28681 caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg ctaacttata
    28741 agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg gggaagcaat
    28801 cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa atggctaaga
    28861 aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg
    28921 ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca ttgccaacaa
    28981 tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa
    29041 aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat
    29101 tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa
    29161 tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt gaagatattg
    29221 acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct tcattaggcg
    29281 atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct aacgtaggta
    29341 tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa atcaagttca
    29401 tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt ggcgcaaaag
    29461 tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa atcttgatcg
    29521 gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt aactctctag
    29581 tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc gacaaacttg
    29641 tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa
    29701 tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca tcagtaaaat
    29761 tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat
    29821 ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag
    29881 aaggcgcaac aacaatcatc ggtggcggag actcagccgc agcagcagaa caattaggat
    29941 ttgctgacaa attcacacac atctcaacag gtggcggcgc aagcttggaa ttgcttgaag
    30001 gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag tttgctcaat
    30061 tctgacgagt aatcgaacaa aaggtgaagg gagttttcat gactccctag ccatttttca
    30121 ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg
    30181 aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa aatcccttca
    30241 aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga attaatgaca
    30301 tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga aaactcaggt
    30361 gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca ttatgtgatc
    30421 atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat caataaaaaa
    30481 gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgcggcga atctttagaa
    30541 acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc tggtttagct
    30601 gatctttcag ctgaacaggt gtcaaacatg gttattgctt atgaaccaat ctgggcaatc
    30661 ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa
    30721 acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca atacggcggt
    30781 tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga cggtgcttta
    30841 gtcggtggcg caagcttgca agcagattca ttcttgtcat tgttagacgc tgtaaaataa
    30901 ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa attaactaaa
    30961 atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac aaaacatgtc
    31021 aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc caacaatcga
    31081 agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt caggtgcttc
    31141 aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt atggcggtaa
    31201 aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa tcattggcta
    31261 cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg gaactcctaa
    31321 caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg cacgtgcagc
    31381 tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca caaaagtatt
    31441 gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact caatcgactt
    31501 ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc tacgtatggg
    31561 tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag caacttctgt
    31621 tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat
    31681 catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc ttgctatgga
    31741 tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag ctgactcagg
    31801 cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag tttctaaata
    31861 tccaatcatc tcaatcgaag atggattaga cgaaaacgac tgggacggat tcaagaaatt
    31921 aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg taacaaacac
    31981 tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa
    32041 ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag aagctggcta
    32101 cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct ctgatatcgc
    32161 tgttgcaaca aacgctggcc aaatcaaaac tggttctcta tcacgtactg accgtattgc
    32221 taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag aatacaaagg
    32281 tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag atacatgatc
    32341 gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc tctacttgct
    32401 gaaatatcag caggtagaga gtttttttgc aactagcttc tgtatatgat gtttacaaag
    32461 aatggtacaa gaaaagatca tccttgaaaa aggaaagcta acaataacta gcatcatata
    32521 agagctttta aggatagaac agtagaatag gaatctatgg gcaagtcctc tcgtataaat
    32581 aaaataggag ggaagtagca gcatattttt cataaagtgg ctctctttct tcatttttac
    32641 tttggttgct cctatttgaa aattcgagca cactcaaata caataaaaag aaatggcaag
    32701 ggagaggatt agatgaaaaa agaagaaaaa atcgcgattt tacaagagat tatccgaatt
    32761 aaatcagtga atggaaacga aggtgaagta gcagcctatt tgaataagct tttggcaaag
    32821 catgacataa caggagagat tgtttcttac agagatggta gagataattt gattgctcgt
    32881 taccaaaagg gccaatcagg aaaagtgtta ggcctatctg gacatatgga tgtcgtggcg
    32941 gctggtgatg aatcgtcttg gacctatgca ccctttgcag cagaaatcca tgggaaccgt
    33001 ttgtacggac gtggtgctac agatatgaag agtggcttgg cagcaatggt gattgccatg
    33061 atcgaactga aagagtcagg aaaaccattt aacggaacag tcaaattatt agccactgtc
    33121 ggagaagaag tgggtgaact aggtggggaa cagctcacga aagcaggcta tgtagatgac
    33181 ttagatgcct tgatcatcgg agagccaacc aattacagtt tgatgtatac gcacatggga
    33241 tcaatcaact atacagtcac ctcgcacgga aaagaagcgc atagctctat gccagaccaa
    33301 ggttataatg caatcaatca cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat
    33361 cacctcgctg aaacaatcga gaatcctgtc ctaggaaaaa caatccataa tgttacattg
    33421 atcagtggcg ggaatcaagt caacagcatc ccaagccatg cgcagctcca agggaacatt
    33481 cgatcgattc cagaataccc aaatgataaa atcattgcat tgctgcaatc aatcgtcaat
    33541 gaattgaatc aagagacaga ctatcatttg gaattgatga tcgattacaa caaaatacct
    33601 gtcaaagctg atccagattc tcctttgatt cattgcattc agcaacaatt cagccagcca
    33661 ttacctttag ttggagcggc agcaacaacg gatgcagccg aatttacgaa agctaaccat
    33721 tccttcgact ttgtcgtctt tgggcctggt gtcgttactt tgcctcatca agtagatgag
    33781 tatgttgaaa tcgataatta tttggatatg atcgaaaaat atcaaggaat tattttatcc
    33841 tacttagcgt aagatttttt taat
//
LOCUS       DAHUWM010000006        33004 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHUWM010000006 DAHUWM010000000
VERSION     DAHUWM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.006"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..810
                     /locus_tag="NK788_000174"
     CDS             <1..810
                     /locus_tag="NK788_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCH6768553.1"
                     /translation="LSGCSKWIDRGESITAVGSSALQPLVETVAETYQTENPGKFINV
                     QGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIVNKDIGVK
                     DISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGETAVQAQE
                     QDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWTIWAYEHM
                     YTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE"
     gene            925..1845
                     /gene="pstC"
                     /locus_tag="NK788_000175"
     CDS             925..1845
                     /gene="pstC"
                     /locus_tag="NK788_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCH6768554.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1845..2729
                     /gene="pstA"
                     /locus_tag="NK788_000176"
     CDS             1845..2729
                     /gene="pstA"
                     /locus_tag="NK788_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCH6768555.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2744..3550
                     /gene="pstB"
                     /locus_tag="NK788_000177"
     CDS             2744..3550
                     /gene="pstB"
                     /locus_tag="NK788_000177"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCH6768556.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3566..4324
                     /locus_tag="NK788_000178"
     CDS             3566..4324
                     /locus_tag="NK788_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCH6768557.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4337..5014
                     /gene="phoU"
                     /locus_tag="NK788_000179"
     CDS             4337..5014
                     /gene="phoU"
                     /locus_tag="NK788_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCH6768558.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5252..6283
                     /locus_tag="NK788_000180"
     CDS             5252..6283
                     /locus_tag="NK788_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCH6768559.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6280..7047
                     /locus_tag="NK788_000181"
     CDS             6280..7047
                     /locus_tag="NK788_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCH6768560.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7147..8715
                     /gene="liaX"
                     /locus_tag="NK788_000182"
     CDS             7147..8715
                     /gene="liaX"
                     /locus_tag="NK788_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCH6768561.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9012..9332
                     /locus_tag="NK788_000183"
     CDS             9012..9332
                     /locus_tag="NK788_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCH6768562.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9332..9682
                     /locus_tag="NK788_000184"
     CDS             9332..9682
                     /locus_tag="NK788_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCH6768563.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9849..10787
                     /locus_tag="NK788_000185"
     CDS             9849..10787
                     /locus_tag="NK788_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCH6768564.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10802..11632
                     /locus_tag="NK788_000186"
     CDS             10802..11632
                     /locus_tag="NK788_000186"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCH6768565.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11676..12701
                     /locus_tag="NK788_000187"
     CDS             11676..12701
                     /locus_tag="NK788_000187"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCH6768566.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12716..13654
                     /gene="galU"
                     /locus_tag="NK788_000188"
     CDS             12716..13654
                     /gene="galU"
                     /locus_tag="NK788_000188"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCH6768567.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13779..14219
                     /locus_tag="NK788_000189"
     CDS             13779..14219
                     /locus_tag="NK788_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCH6768568.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14222..14824
                     /locus_tag="NK788_000190"
     CDS             14222..14824
                     /locus_tag="NK788_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCH6768569.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14883..15989)
                     /locus_tag="NK788_000191"
     CDS             complement(14883..15989)
                     /locus_tag="NK788_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCH6768570.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16199..17200
                     /gene="ccpA"
                     /locus_tag="NK788_000192"
     CDS             16199..17200
                     /gene="ccpA"
                     /locus_tag="NK788_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCH6768571.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17537..19882)
                     /locus_tag="NK788_000193"
     CDS             complement(17537..19882)
                     /locus_tag="NK788_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCH6768572.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20161..21063
                     /locus_tag="NK788_000194"
     CDS             20161..21063
                     /locus_tag="NK788_000194"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCH6768573.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21276..23051
                     /locus_tag="NK788_000195"
     CDS             21276..23051
                     /locus_tag="NK788_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCH6768574.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23117..24703)
                     /locus_tag="NK788_000196"
     CDS             complement(23117..24703)
                     /locus_tag="NK788_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCH6768575.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            24954..25178
                     /locus_tag="NK788_000197"
     CDS             24954..25178
                     /locus_tag="NK788_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCH6768576.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25320..27071
                     /locus_tag="NK788_000198"
     CDS             25320..27071
                     /locus_tag="NK788_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6768577.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27071..28855
                     /locus_tag="NK788_000199"
     CDS             27071..28855
                     /locus_tag="NK788_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768578.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29181..30032
                     /locus_tag="NK788_000200"
     CDS             29181..30032
                     /locus_tag="NK788_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6768579.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30086..31837
                     /locus_tag="NK788_000201"
     CDS             30086..31837
                     /locus_tag="NK788_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6768580.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            31841..32011
                     /locus_tag="NK788_000202"
     CDS             31841..32011
                     /locus_tag="NK788_000202"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6768581.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(32051..32953)
                     /locus_tag="NK788_000203"
     CDS             complement(32051..32953)
                     /locus_tag="NK788_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6768582.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        10497 a         5867 c         6967 g         9673 t
ORIGIN      
        1 ttatcaggct gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct
       61 gctttacagc ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa
      121 ttcatcaatg tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca
      181 gtcgacatcg gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca
      241 ttagtcgatc atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc
      301 gtaaaagaca tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg
      361 agtgaagtcg gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga
      421 acccgtagca cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa
      481 caagattcaa gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat
      541 gttgcttttt cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg
      601 acagatgaaa atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca
      661 agagatcatc caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta
      721 caagataaca tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt
      781 gattggcaag gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa
      841 acttttacat gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta
      901 aacctgatta agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca
      961 aaacgttcaa aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc
     1021 gtgttggttg tattatctat tttttatttt gtagctagta aaggattggc cacctttttt
     1081 actgataaga tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc
     1141 ggagcaaatg ggaaaccaat ggtaggggca ttgcctatga tcacaggttc gtttattgtg
     1201 acctttttat ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg
     1261 gagatctctc ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt
     1321 attccttctg tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt
     1381 atttttggcg ggactgggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc
     1441 ttgccgactg tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg
     1501 gaagcttcat tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga
     1561 gcagccgtgc ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa
     1621 gccttagcta tccaaatggt catcggtaat gcagcattga tgccaacagg tcttactact
     1681 cctgcttcta cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca
     1741 gttgaaaata atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt
     1801 aatatcgtga tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct
     1861 cagataaaat tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag
     1921 cagctttact actttatatc ttagttcggg gaataccgca catttcatgg tccttcttga
     1981 ccgaaccttc caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga
     2041 tttatttgct tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct
     2101 atctttcaga atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa
     2161 tcttgagctc gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc
     2221 aattccagta tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc
     2281 ctcttttgac tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag
     2341 caggattggc cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag
     2401 cgacaccagg gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag
     2461 ctgcattgat ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc
     2521 cattaagtgt ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata
     2581 tttggaagat caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt
     2641 ctgccgtgtt gattattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac
     2701 gattataccg aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg
     2761 gaatgaaaca aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga
     2821 tctacatgtc tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa
     2881 aaataagatc actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc
     2941 gttgaatcga atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca
     3001 aggcgtagat attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat
     3061 ggtttttcaa cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt
     3121 gaaacagcac ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa
     3181 acaagctgcc ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc
     3241 tggcgggcaa cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt
     3301 gctgctagac gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac
     3361 acttgtaaac ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc
     3421 agctcgtatc agcgactata ctgccttttt ctatttagga aaagtaatgg aatatgacaa
     3481 aactaacaag atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca
     3541 ttttggttaa gacggaggcc tcaacatgac aaaagagatt atttcatcta aagatcttca
     3601 tctgtattat ggaaagaaag aagcactaaa agggatcgat ctaagcttca atcaagggga
     3661 aatcactgcg atgatcggtc cttctggttg cggaaaatcc acttatctgc gttctttgaa
     3721 ccggatgaat gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa
     3781 agacatctat ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt
     3841 ccagcagcca aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt
     3901 aaaaggggaa aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc
     3961 gtctgtttgg gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg
     4021 gcagcagcag cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt
     4081 ggatgaacca acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt
     4141 agaattaaga gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg
     4201 tatttcagat aagacggcat tctttttaga tggtcacttg atcgaataca atgatacgaa
     4261 gaagatattc atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg
     4321 ataaggagga aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa
     4381 tcagttctac gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata
     4441 catcaaacac gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa
     4501 catggaaaca cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac
     4561 aactgatttg cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc
     4621 cgatcatgcg gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc
     4681 ggaaatcgaa aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt
     4741 cctagttgct tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca
     4801 aagagtcaat gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa
     4861 tcctgagaca gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat
     4921 cggagactat gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac
     4981 tgagttaaat acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt
     5041 ttaggaaaag aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa
     5101 gaacagtcaa acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag
     5161 atgcaagctt ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac
     5221 ccttttcatc atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat
     5281 attttttatt tgtgtgctta cttcatgggt tattcagcat caagccgctt tatatgaagt
     5341 acctgtcatc gagatagaaa aagtacgaat aaaagaggat catcaattga ttacaggaaa
     5401 actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa
     5461 tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga
     5521 tgaatggcag ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt
     5581 cattggcgcc atgttgctta ttggtggaaa acagggtcta tttgctttag gaagtctcat
     5641 catcaatatc gttttacttt tacttttgtt gctcatcttt actaaaacag aagggatttc
     5701 tctattaaat atatctatcc tgttcgtcgt tatagggatg attattagca tattggcttt
     5761 agatggatgg aatagaagca gtataatcaa aatttcagct gcagttacga gtgttttttt
     5821 agcatttttt atttgtcttt tgacaatgaa tcattggaaa gaccaaggac tgcgctatga
     5881 ggaactgaat tatctgacac gaccgtatcg ttctgttttt ctttctagcg tactgattgg
     5941 aacagctgga ggaacactcg ataccgttat cacagtcatt gcgacattag aagaattgac
     6001 taagcgacaa ccagaaataa cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg
     6061 agatgtgatt ggctcgatga ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc
     6121 atcgcttctt ttatatttag gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt
     6181 atcattggaa tttttacgcg tcattgctgg cagcttagga atagtattat cgatccctat
     6241 ctcggtcctc ttttttctga ctgtaaggag gctgaaacga tgaatgtact tattatactt
     6301 gttgggattt tagcaatcag cgtgctactt attggcggta cgcaaggtat gtatattctt
     6361 ttaggcttat tcatcaattt agggatcttt ttcctgttgt tattcggtta tcatcagaag
     6421 tggccgatac tggcattgtc ggtcatagga tttttattga ttgctgtggt tatcttattt
     6481 tttatcaacg gttataatct aaaaatgcgt gcagcttttg tgagtatact gatcttttta
     6541 ttttgtttcc tactattgct tcctatcact gattttttag caatccaagg ttttacatcg
     6601 gtagaactag aagaattatc tggcttagat aagactttag cgattgattt tagattactt
     6661 acgagatcgt tgcttctgat tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt
     6721 tctagtggga cattcgaagt atatcaggca aatccgcaat tatcttttta ccaattgcgc
     6781 cattctagtt tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac acttcttttt
     6841 gcattcatgg gaagttcttt agcgttgatc ctctggttta ttgatttaga cattcctttt
     6901 cagcaagtga tcaatgaaaa atcatttgtt ctagaatata cgatggctat tcttaccaca
     6961 ttgtccgcac tacttgttct tcctttaacc tgcgtgacta cagcatatct ctttacaaaa
     7021 aagaaagaac aactcaagat ggagtaaaag ttacaactta agtcctatga catttttact
     7081 gaatcagtgc ataatatgga tgtaaggaaa agcttgcaaa caattttgct taaggaggca
     7141 atttccatga aagaaagaga acgtatttta gatttagtga aaaaaggaat cctttcgact
     7201 gaagaaggct tagatttatt agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa
     7261 aaagaagcag acaaggtaac tgctagtcat aaagaaaaag atcaggcaag tcaattgatc
     7321 gataaacttg aaaacggcga agaagaaatt tctgagccag ttgatccaaa ggaaagagaa
     7381 agacaagatc aagaaaactt agaaaagatc ttagatgaac tagcaactga agcgaataaa
     7441 acttcggcgc gtctagatga agtaaatgct caattcgctg acaataaggc agcacgcaat
     7501 gagaaacagg aagcattgat gcagctgaat acaaaagaag aattaggcga gttgacagaa
     7561 gaagaattag cacagcgtca gacgttagaa gcagaaatca aagaattaga agctagtggc
     7621 gatacactgt tagaagaaca attgaaatta gaagcagaat taaaagatat ccgtaagaat
     7681 caatggtctg agaaaaaaga aacgtttact gataagtttg aattgccaga tgattggaaa
     7741 gatcaagcaa cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag
     7801 ttaggcaaat tcttgaagaa aacattccaa actgtttcag aaacagtgaa tgacaacatg
     7861 gaatggaaag atgtcagctt acgtgtccct gggattgcga caacgaaatt tgagcatgaa
     7921 ttttactatg aagctcctgc agcaagtatc cttgatatca aggcagcaaa cggaaacgtg
     7981 acgttaaaaa cgtgggacag caacgatgtg aaagttgaag ctaagatcaa actttacgga
     8041 aaaatgggtg ctgaaccatt tgaagcattc tctgaacgga gccagattga ggttaatgaa
     8101 gatcatatct ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt tttctactta
     8161 cctaaacgag tatacgacca cgcagctatt aaacttctaa atggcaacat catgattgaa
     8221 acattggaag ctaaagatat ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa
     8281 ttaactgcaa cgatgctgga agtagaaggt gtaaacggca atatcgatat tcgaaacggg
     8341 aacatcttag attctattat cgaaacagta aatggtacag tgacatttgg tgcgacagca
     8401 gaaaaccttt ctgtgtcttt ggtaaatggt gatgtacgtc ttacgattaa agaagataat
     8461 ttgaaaaaag tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt accagatggc
     8521 atcggtttgg aaggacatgc taaaacaagt cttggaagta tcaacagccg tctgtccaat
     8581 tacgaagtag ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt
     8641 tcagatggtg aaatcgcgca agtgcaactt tcaacgacaa caggaagtat ctatttaaaa
     8701 gatacagata aataaagagg ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat
     8761 tttgtaaaat agcttcccct attttacaaa attgtgcctt attgccgata gtctatctct
     8821 tgaacaccgt ttaccaagag attatgaacc tgccaattat cgccgagtca caaggaataa
     8881 tttcagaaaa agctcgtcac gttttatgaa tatggtaatt tgtgaccgag aattttgcag
     8941 aaagacaccg attaagaaga aggttgtcac aacgattttc ttcatttaaa atagtagaac
     9001 agaggtgaga catgatgaaa aaaaagttga caaaatctaa taaaaatgtt gttttgacag
     9061 ggactttagc aggaatagga gaatacatcg ggattgatcc gactgttatc cgtgtgcttt
     9121 atgtattcct gagcttagtt gcttttggaa gtccaattat tttatacatt ttattagctt
     9181 tgattatccc atctgcgcgt tctgcacgaa caaactatgg acataacaat gcatattatg
     9241 aaaaaaacaa ttatcgtgaa caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa
     9301 aaatcgatga tgacgaatgg agtgactttt aatgtcgtac tttcaacgta ttttagttaa
     9361 tacgttaaca ttcatatcat tggctgttct ttttcctgcg atggtccatg tgagcagtat
     9421 ttggacggcc gttcttgcca gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat
     9481 tttgacgata ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat
     9541 caatgcagcg attcttaaaa tgacctcctt ttttgtggga gcagaacatt ttagtttttc
     9601 ttcattcggt gcagcgttgt tagtagctat cttgatgtcg cttgtcaatg taattgtcag
     9661 cgaacataac agtagaaaat aaaagaaagc tgtgccgaac tatcggcgca gtttttttat
     9721 ttagaatcag gaatgcgttt tctattacat tggtaattaa ataaaaacgt tttttttaat
     9781 tggtagtact tttggctaga caatgataaa atgatagaga acactctaat agaaatgagg
     9841 tagcatgaat gtcagaagtt gtaaaagtta gccaattagt gaaaaaatta tctttggaaa
     9901 tcgtgacagg cgacgagaaa agcttagacc gtgtcatcgt aactggggac atctcacgcc
     9961 caggactaga gttgactggt tattttaatt attattccca taatcgttta cagctatttg
    10021 gcagtaaaga aatcagtttt gcggaacgga tgatcccaga agagcgtttg atcatcatga
    10081 gaagattatg tagtgaagat atgcccgcat ttattatttc aagaggatta ccagctccag
    10141 aagaaatgat ccaggcagca aatgaaaatg ggatagctgt actgcgttct cccatctcaa
    10201 cttctcgttt attaggagaa ttatccagtt atttagatgg tcgccttgcc cctcgaacaa
    10261 gtgtccacgg tgttttagtg gatgtttacg gattaggtgt actgattcaa ggtgacagtg
    10321 ggataggaaa aagtgaaaca gcgttggagt tgatcaaacg cggccatcga ttgatcgcag
    10381 acgatcgagt agatgtttat cagcaagatg aattaacggt gatcggagaa ccgcctaaaa
    10441 tcctggaaca tctgattgaa atccgaggaa tcgggatcat tgatgtaatg aatctttttg
    10501 gtgcgagtgc ggtacgtggc tctatgcaag tccagttggc tgtttatctg gaagcatggg
    10561 caaaagataa gaaatacgac cgtctgggct ctgaagacac gaccgttgaa atcgcggaag
    10621 tgggcattcc acaaataaaa atccctgtca aaacaggacg aaacgttgca atcatcattg
    10681 aagtagccgc gatgaatttc cgcgcacgta caatgggctt tgacgcaacg aaaacatttg
    10741 aagaaagatt aagtcgcttg attgaagaaa actcaggcaa cgactagcaa ggagtgacaa
    10801 tatgttagca caaatcaatc gagttgcttt tgagcttgta gggattccta tctactggta
    10861 tgcattgatc atcgtatcag gtatcattat cgctatgtgg cttagcagca gagaggctgt
    10921 tcgggtagga ttaaaagcag atgatgtgac agattttatg ttgtggggat tgcctatctc
    10981 cattattggc gctcgcttat attatgtttt atttgatctt ccccagtata tagctgatcc
    11041 aatccaaata ttcaatatcc gttcaggagg cttagctatt tatggcggac tgattgcagg
    11101 cgcgatcacg ctgtattttt ttactaaata tcattttatt tctttgtgga cttttttaga
    11161 tatcgctgca ccaagtgtat tattagcaca ggcaatcggt cgttggggaa atttcatgaa
    11221 ccacgaagca tatggaccag acacgacgcg aagcttctta gaaagtctgc atttaccgca
    11281 attcatcatt gataatatgt atatcgaagg gatctatcgt caaccaacat ttctttatga
    11341 atcactttgg agtttgattg gatttatttt gcttttgttt ttacgaaaga aaccacacct
    11401 tttgaaaaaa ggcgaagtcc ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt
    11461 tattgaagga ttacgtacag acagtcttta ctttttaggt gtgatccgta tttctcaatt
    11521 attatctgct gtgttatttg tcggtacatt gattctattt atttggcgca gaaaaaaagg
    11581 aaatttaccg gattatgacc gctcattagg aaaaaatcaa caaattatct gatttttaga
    11641 gcgttaaaaa tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta gcggttttag
    11701 gaccaggctc ttggggtact gctttggcac aagtcctcgc cgaaaacgga catgacgttc
    11761 ggatttgggg acatcgtgct tcacaagtag aagaaatcaa cacccaacac acgaatcaac
    11821 gttatttacc agatcattat ctacccgatt ccatccaagc attcaccaat atggaagatg
    11881 cagtaaaaga cgcagatgcg gtgctattcg tcattccaac gaaagcaata cgttctgttg
    11941 caaaagaact agtgcctaaa ttgaatacaa agccagtgat catccatgca agtaaaggat
    12001 tagaacaggg aacacataaa aggatctctg acatcttgtt agaggaaatc ccagcagaaa
    12061 aaagaaaagc tgttgttgtt ctttctggac caagccatgc agaagaagta gcaaaacatg
    12121 acatcactac gatcacagct gcaagtacag atgcaaaagc agcaaaatat gtacaagagc
    12181 tgttcatgaa taattatttt cggatctata ccaatcctga tgtgatcggt gtagagatgg
    12241 gggcagcttt gaaaaatatt attgctattg gtgctggcgc tattcacggc ttagggtttg
    12301 gcgataatgc aaaggcagca atcatgacgc gcggattagc tgagatcagc cgtttaggtg
    12361 tagcaatggg tgccaacccg ctgaccttta tcggattaag cggaataggc gatctagtcg
    12421 ttacttgcac aagtgttcat tctaggaact ggcgcgcagg caacttgtta ggacaagggc
    12481 agcccctaga agaggtgcta gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc
    12541 aagcagcgat ggaactagcc caaatgatgg aaatcgaaat gcccatcact caaacaatct
    12601 atgaagtgct gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc atgttacgtg
    12661 atggaaaaaa agaaaatgaa ttctcattaa caattttttg aaggagcgaa tgtatatgaa
    12721 agttaaaaaa gcagttattc cagcagcagg attaggaaca agatttttac cagcaacaaa
    12781 agctatggca aaggaaatgt tgccaattgt tgataaacca acaatccagt tcatcgttga
    12841 agaagcgttg aaatcaggta tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc
    12901 aatcgaagat cactttgatt caaatattga attagaaaca aacctaagtg aaaaaggaaa
    12961 gaccgaatta ttgaaattag tagaagaaac gacagatgtc aacttgcatt tcatccgtca
    13021 atctcatcca aagggtttag gacatgccgt tcttcaagca agagccttta ttggaaatga
    13081 accatttgtc gttatgctag gcgatgatat aatggaagac gaagtaccat tgacacagca
    13141 attaatcgat gattacgaac gtactcatgc ttctacgatc gccgttatgc aagttcctca
    13201 tgatgaaact tcaaaatacg gaattatcaa tccaggagaa gtattggaag atgggttata
    13261 caatgtcaaa aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg atttagctat
    13321 tattggacgt tacttattga ctccagaaat ttttgatgta ttgtccgagc aacaaccagg
    13381 agccggaaat gaaatccaac taactgacgc aatcgataca ttgaataaaa cacaacgcgt
    13441 atttgcccgt aaattcacag gtaaacgtta cgatgtcggt gacaaatttg gttttatgaa
    13501 aacaagtatt gaatatggtc taacacatcc agaagtaggt gcacctttac gtgagtacat
    13561 cctgtcttta ggtaaaacat tagaagcaga aggaagcaag atcgatcata atccaaaagg
    13621 aataacagaa atcaaagaag aaaagaaaaa ataacccttt tttctaacga gcttcagctg
    13681 ctaattgaat aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct
    13741 atccttatag aaaagagaga caagaaggga gtttgatcat gtcaggagga gaaattgcag
    13801 gattgatcgc agcaatcgcc tttgcgatac ttgtcgtttt tatcgttcgt gtgttgttgc
    13861 aagtttctaa aactttagaa aaagtagatc agactgtagc agaagcaaat acgacgatcg
    13921 aaatcgtcac aaaagacgta gatcttttgt ctagacaagt agaaggctta ctggtcaaaa
    13981 gtaatgaact gctcttggat atcaatggaa aagtcgcaac gatcgatcca ttgttcacag
    14041 cagttgctga tttaagtgaa agtgtatcag aacttaatca ttctagtaaa aacatcgcga
    14101 cgaaagtcgg cggaatcggt aaaacgacag ctaaagcgac ggttgccgga aaagtcggtg
    14161 ggacagcgat gaaatttttt aaaaacaaaa aacatactga aacgacagga ggaaaataaa
    14221 aatggctaaa aaaggtggat ttttattagg tgcagtagtc ggaggcacag ccgcagcaat
    14281 cgccgcgtta ttgttagcac caaaatctgg taaagaactt cgggatgaat tagctgccca
    14341 agcggatgat ttgatggata tggcttcaga ttacacagat atggcgaaag aaaaatcgac
    14401 tgagttaaca ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggtgatct
    14461 agcaggtagt gtcaaacaaa aagtaagtga atcagcagat gaagcaggaa gtgaatctga
    14521 cgatatcttg ttaaatctta aagaacaatc agctgactta tctaatcgtt tcaaaaaaac
    14581 ggcagaagat atgaaaaaac ctgcagatga attaggaagt attgctaaag atacttcaga
    14641 agatatcatg attgatgtaa aagacgctgc tggtaaagca ggagatactg tcaatgaagg
    14701 aatgtcagaa gcaaaaccag ttgcagatga ctcaaaagaa atgatgaaaa tggcaaaaga
    14761 agatgcgaaa gaagctaaaa aagaaataaa agatacaatg acagattcat ctacaaatca
    14821 ctaaaatcac aaatttatag aataaaaagg tgggagcgga ctagctctca ccttttttac
    14881 tcttattttt ctaaaacaag taattctttt ggtgtagtcg tgaatggcac acagccatcg
    14941 cttgtcacat atacacagtc ttcgatacgt acccctactt tttcaggaat atagattcct
    15001 ggttcaagag agaagcacat cccttcttcg attactaagt catttccttc tacaagggaa
    15061 gggaattcgt ggacggttgt tccgatcccg tgacctaaac ggtgattgaa atattcgccg
    15121 tagccatagc tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct
    15181 ggacggacgg cttcttgtgc ggcaagctgt gcttcaagag tgatgttgta gattttttct
    15241 tggaagtcgg ttggtttttg ataagcaatt gtacgagtag catcactgca atagccattc
    15301 caaacaacgc caagatcgaa taacacaagt tcattcggtg aaatcttcgt atttcctggt
    15361 gtaccatgag ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa agacatttgt
    15421 gaaacacctt gttttttcaa ttggtattca atttctgcaa tgatcgcttg ttcagtcacg
    15481 ccttctttca ctgctttaaa gccaatctca aatgcaacat cggcccattt accagcttcg
    15541 atcagtttgt ctttttcctc tagagttttg atcagttgta gacgttggat gatggaagtt
    15601 agatcagtgt aagaatccgt tcccgtcaag atatcagaga tagcatcgaa gcgctcgaca
    15661 gtcaaggcgt tcttttcaat ccctacattg ccaggctgac catagcgagc acgaagttct
    15721 tttccgatga tctcccaagg gttttcgctg tctaaatagc cacggacatc atacgcccaa
    15781 ctactgtttt gagcatcttc aacttccaaa gcaggagtga aaaggaaagg atcttgttca
    15841 agagggataa aaagagctag tacacgctca tgaggatcac tttcgtatcc ggaaaagtaa
    15901 gcgatgtggc cagggtcgct gatataagcc aattcgactt ggtgttgtgc catccattga
    15961 cgtagttcat tgatttttac gttattcatt tggcaaccta ctttctaaat taattttgta
    16021 ttcaataatt gtaccactga tttattaaaa gaccaacgtt ctaaaaactg aaaaaagtaa
    16081 aaattttcat gaaaacagag ataaaacggt tgcaattctt gagattattt gatattctat
    16141 tttagaaatg taagacgtat tttcagaaaa catataaaat atgaatagga gaacacaaat
    16201 ggaaaagcaa acaatcacta tctatgatgt agcgagagag gcaaatgttt cgatggctac
    16261 tgtttcacgt gtcgtaaatg gcaatccaaa cgttaaacca gcaacaagaa aaaaagtctt
    16321 ggaagttatc gatcgcttgg attatcgtcc aaacgcagtt gcgcgtggct tggcaagtaa
    16381 gaaaacaaca acagttggtg taatcatccc tgatgtcagc aatatgttct ttgcttcttt
    16441 agcacgtggt atcgatgatg tggcaacaat gtacaaatac aatattatct tagcaaattc
    16501 agatggcaac gaccaaaaag aagtcaatgt attgaataac ttactggcta agcaagtgga
    16561 cggcgtgatc ttcatgggtc accatatcac agacgaaatc cgtggagaat tttcacgttc
    16621 aaaaactcct gttgtattag ctggctctat tgatccagat gaacaagtag gtagcgtaaa
    16681 cattgattat gtatccgcaa caaaagatgc agtaaacaaa ttggcaaaaa gcggcaataa
    16741 aaaaatcgct tttgtcagtg gtgcgttgat cgatcccatc aatggacaaa atcgtctgaa
    16801 aggctataaa gaagcattaa gcgaaaataa cctaccatat agcgaaggat tgatctttga
    16861 agcacaatac aacttcaaag atggtctatc tctagtcgat cggatccata atagcggtgc
    16921 aacagctgtc tatgtttcag atgatgaatt agccatcggt attttggatg gtttattaga
    16981 ccgtggcgtg aaagtaccgg aagaattcga aatcatcaca agtaacaatt cactactgac
    17041 agaagtagct cgtcctcgtt taacaagtat cacacaacca ttatacgatt taggggcggt
    17101 atctatgcgc ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat
    17161 cttgccttat ggaatcgaag aaaaaggatc tacaaaataa taaaaggaca accattcaga
    17221 ggttgtgtgc ctgccgttta actctgcgtt acaaggagca atttcaaaaa acagcatctc
    17281 atgtttgatg aatattgtga ctcgtgattc ggagttggca ggagaacacc gtttatttgg
    17341 gaaaaggggt tatgacaaaa ttttgttata atcctttttt tgtgtcttaa aaaagagaga
    17401 aagtggttga aattacattt atcgtatata aaacaatccg tgtatagatg ctgcattttg
    17461 ttcaccacca tcaaaaaaat aagggactgt gacaatacta ttgtcacaat cccttattac
    17521 tgtgtattct attaaatcaa tcatctttct catctgattc tttagttgct tgattcgctc
    17581 ttgcataagc tgaagcggtg ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc
    17641 caaatttaaa ggcgcttttc ttcggtccat ttttcgccca tagcgattca actgtttttc
    17701 ctggtgtttg gatggatttt ttattgacag taacttttcc aggtgtttga ccagtaaatc
    17761 cagatacttt tttcttcatc acatcagatg ataattcgaa tttctcatca gtgccaaaaa
    17821 tgtcaggttt tgcttggtaa gcttgattga tcagattggc aagataatta gccgaacgta
    17881 tccttgcttt gctgtccatt ccagtattgt catcgtgccc tgcccagcta ctgatggtaa
    17941 tagacggagt agagacaatt agccatgaat ctcggtattc attggtagaa ccagtttttc
    18001 cgacccaatc tgcttttcct agatttccgt ttaagcttga gatggcatct ttgaatggtg
    18061 tagtgatttt tgcattgatc acactacgca tcatgtcatt catgatcgat gccgttgctt
    18121 ccgaatagat ccggacagag ttgctttcgt gtttataaat tacatttcct gcgttatctg
    18181 tgatggaatc gatgatatag ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc
    18241 cgttggtctg ttgaagcgtc gtgacatcta ctgtacctaa aggagcagat tcaacgcccc
    18301 aattgtcatt tgccgggtag ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg
    18361 atccaccgga attcaacaca tcttgatata agtgataagc cgggatattg ttggaccatt
    18421 ctagtgactc acggacggtt tggaaggtgt tcgatccttc gttagtagca ttgacgattt
    18481 tctcgccagc attttctcct tcttgccaag ttgttgcata atctgataca cgggattctg
    18541 atccgataag ccccatgtcg attgcaggtc cataaacaag aactggtttg attgatgagc
    18601 cagcttgacg ttctgtatcg aaggcatggt tattttgatt ctgactgtaa ttccgtccgc
    18661 caacgaatcc gtagatgcgg ccagttctgt tatccatcaa tacatttccg gtttctactt
    18721 gactgccagc accatcatct aacatgtatc cataattttc aacgccattt tgcaatgcag
    18781 catagacatc tttatcgatt gtcgaatgga ttgtatatcc tttattttgg atcgtttgct
    18841 gtgccttttg ataataagcg tcactgaccg atgaatcaga catatcagcg ttgtcttttt
    18901 cagccaattg ttgtgcaatg attcttgttg cttcattcat cacagtataa tacaagaatt
    18961 ctctgtcagt ctgttctgcg atttgttgtg gtagaaagtc ttttgtaagg tcataggctt
    19021 ttgcttcttc gtattgttct ttcgtaattt ggccttcacg atacatactg aataaaacaa
    19081 aatcttttct ggcaaggccg gcagaaagat cttcttttag cgcgcctgtg ttcgtgtatg
    19141 gactatacgt gatcgggctt tggggtaatc cagcgatata agcagcttgc ggcagcgtca
    19201 catcttttgc atctacacca aagattcctt ttgcagcttc ctggactcca gcaatgtttt
    19261 gtcccttatt attacgacca aatggtgaga cattcaaata cgtagcaatg atctcatctt
    19321 ttgaaaagta tttttctact tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg
    19381 ttgtttcatc tgtaaggatc tgctgtttga ctaattgctg tgtcaaagtt gatccaccag
    19441 aagagccaat accagtagct tcagatacta atgctcgtaa aacagcttta ggaacgtaac
    19501 cttggtgttt atcgaagtat tcatcctctg tgctgataat tgcagttttc aataaaggtg
    19561 aaatcttatc agatgaaata gtcgtgcgca tcagatctgt ctggattttt gatatctctg
    19621 tattatctgc ataaacaatc tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg
    19681 cttttttagt cggcaattgt gtgtcttcga cgagataggc aaaatagcca gcgcctattc
    19741 caatacctag agctgcgaac atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa
    19801 aaactctaaa aataatctta ggaacaaacc atttttcttt ttttcttcta ttctgttttt
    19861 ttcgagtatt tttgcgtggc aaaagggtca cctcaactaa tggttttatt tcgctaatta
    19921 tagcaaataa tagaacaaaa aaacagacaa ggctgttttt ggcaggaatt tcttttttaa
    19981 aagagttaaa acgtgagaaa atgatcttta ttataaaaga gttcttaagc ttttctttag
    20041 agaaaatttc agttactatg gagaacaggg caaagaaaaa tcaatcattt tctttgtata
    20101 tcatcttacc aatatatagg aattgttagt aacaattgcc tttcaaggag gaatccatcc
    20161 atgttattag gctcacacgt cagcatgagc ggaaagaaaa tgctgttagg agcagcagaa
    20221 gaagcagcca gttatggatc ttcgactttt atgatctata caggcgcccc tcaaaacact
    20281 agaagaaaag ccatcgaaga gatgaatatt gatgccggaa aagcgtacat ggcagaaaaa
    20341 ggtctaacaa atatcgtcgt tcatgcccct tatatcatta atttggggaa tacgatcaaa
    20401 ccagaaatgt ttccttttgc cgtccaattt ctgagagaag agattgttcg tgcggaagct
    20461 ttaggcgcta ctcaaattac acttcaccca ggtgctcatg taggggcagg aacagaagcc
    20521 ggaattgcta gaattattga aggcttgaat gaagttttga caaaagaaca gacggcacaa
    20581 atcgctttgg agacaatggc aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta
    20641 gcgcaaatca tcgatggagt aaaattgaat gaaaaattat ccgttacttt tgacacctgt
    20701 catacgaatg atgccggtta taatattaaa gaagactttt caggtgtaat ggaagagttt
    20761 gataagatca tcgggttaga tcggcttaaa gtactccata tcaatgattc taaaaatccg
    20821 caaggttcgc ataaagaccg gcacgccaat attggttttg gaacaatcgg attcgacacg
    20881 ctgaataaga tcgtccatga tccaatgttt gacaatattt cgaaaatctt ggaaactccc
    20941 tatgtaggca aagataaaaa gaaagcttac gcaccatatg gaaaagaaat cgccatgttc
    21001 aaagcgcaaa cattcgatcc agaagtgttt cctgagttat tagcagaaga agtgatactc
    21061 taagattttc tatctgacag cagcaaaaaa ggaccaggca cattgccttg tccttttttg
    21121 tttaagaaac aaaggagtac tttttgcagt ctttttcatc atgcatcttc tcccccctct
    21181 ttttaaattt agacaaacaa ggtatactgg tagaaaagta aaaaaacgaa aagtttcaca
    21241 agcaagtggc aaaacgtgat tggaaggacg aaaagatgct gaacacttta aaacccttga
    21301 tcaaccaact aaaaaagaaa catacatact ttattcttgg catagcccta ttatttacaa
    21361 ttattcgttt gtatttatat agtaaagcaa tctatggaac agacatgtcg gcaacctatg
    21421 atgaccagct gctgattcgc tatgctcaaa atattttgga cggtaaatgg ctaggggagt
    21481 atacgactac aacactttcc aaaggaatct cctacagctt atttttagta cttgcacata
    21541 aactgcatct ttcttatgca gttttgttcg gcatgttcag tatttttgcc agtatattgg
    21601 ttgcactcgc gtttaaaccg attgtgaaga acaagtatct tcttttaagt atctattttt
    21661 attttttgtt ttctccgatt agttttacac atgaatattc cactcgaaca tatcgtaatg
    21721 ccctcgtgat tccggctgtt gagatcatta tcgcttgttt attagctatt tattaccgca
    21781 aatacggttc tagcagaaaa ttgctgccat ggtttttagg gctttctgtc gcttttcctt
    21841 tcttttggtt cattcgcgaa gattctatct ggcttcttcc ttttactgta gtagctttgg
    21901 gtatcagttt cgtccagatc ttgctagggc aagacttttc tttcaaagaa gtaaagaagt
    21961 taaaaaaacg actctttctt ctctcgaaac gggaatggct gaaactttca ttgtttttac
    22021 ttcctatagt tggcttttcc atcgtctatc aatggatcga atataaaaat gagacaacat
    22081 acggtattgc aaccatcaat gaccgttcca gtggagaatt tggacgtttg atgaaacaac
    22141 tgatccgaat agatgatgaa acgaacttga ataaagaaaa cagtgagata tgggtctcac
    22201 atgctgcact aaataaagca ttagccgcat ctcctacatt ccatactgtt tctacccgta
    22261 ttgacgaatt atatacgact cacgcgtgga ctcaaggtgg aaaagtaaaa gaattacccg
    22321 gagatatcat cttttgggca ttgcgagatg tggtaagcga atctggttat taccaaaaca
    22381 atgcaaaaga aaccaatgat ttttggaaaa aagtcaacca agaattagaa gaagcctatc
    22441 acaagggaac aatcaaaaag aaaaaagagt tctatctgat gggatcagga gatggaaaga
    22501 cgatgagtga tctgccaatc gtattcgatt tccttcaatc aggatacgct tatgcatggc
    22561 gatataaaga tttccatcaa ggaggaaatt atagtttcgg aacgcaagaa caagtcaaac
    22621 aggctcaaga attattaaac cttccgctaa tggagaattg gatcgatagt aatgaaccga
    22681 gaacacctaa tttccaatta acaaaatcag caaaagtcgc gaatgtcatc ataaaaattt
    22741 accaagttct ggctattccc gtgctgattg cttcaatagc agggatatta cttatgatac
    22801 taggaaccgt atttgcaaaa gctgatcgtg cctattatag ttcctctttg attgtggtca
    22861 ccggattaat actgacacaa ttggtttttc ttttcggtgt ctcatggttt tgttcttatg
    22921 cgccagaaaa aaaggactat tttttaagtg tctacactgg tgcaggcgtg ccattaacgc
    22981 aattcatcgt agtaacaagc atgctagttt tcagtaaatt aaatctgaaa aaagctttat
    23041 cttctaaata aaaagaccct tggtagctag atttttaacc taactatcaa gggtctttgt
    23101 gtggaaaaat cagtttttat tcgatttctt ctaccgtttc ctgcaaaagc tgttcatatt
    23161 ttttgtttcc tttatataaa tcaccaatcg tccatgccaa caggagaagg acaagaacaa
    23221 tgattacata agcgatgtga tcagccaatg ctagttgagc ggaagtggct tgctcgccga
    23281 agaaactttc gatctgtcct ggtaatcctc gtaagttcaa gtaagttaga ctaatcacag
    23341 agaaccagcc aagaagtttt acgatgaatg aatttttaaa tcggctaccc atctccgctt
    23401 ggctgttggt catcatcagt aaaggaatca tcgaaaacgg caacgcaaaa gccaagaaca
    23461 cctgggattc attcattaag ttattcaaag cggcatgttc ttcgatcgtt ccttttgtac
    23521 ttgtaaacaa aacacaaatc aatacaggaa tcacagaaag caatcgtgtg accagtcttc
    23581 ttagccaaat cgggatacgc atatggatga atccttccat gatgacttga ccggtcaacg
    23641 taccagtaat agtagagttc tggccagaag caagaagtgc tacagcaaat aaagtggaca
    23701 agataccaga ttttgctaca gcaatcaaaa taccattgct catagctgat ggatcagata
    23761 aagcttcata cagaccaaaa aaggaaggat cttggacaga accggttttg aagacagcga
    23821 cacccatgat caatagcagt gcattgacta gaaaagccat tgacaactgg atattagaat
    23881 cccaagtaga aaaacgtaca gcttgtgcta catcttcttc gttttggtga tcgatcttcc
    23941 gagtttgtga aactgctgaa tgaagatata agttatgcgg catgactgtt gcaccaataa
    24001 tccctaaagc tccagtcagc ggagtttcgc cgccgatgac cggtgagtct gagaatgtat
    24061 gactgttagg gataaagcca gcaaataaac tgtgccaatc tgggttagac aagccaactt
    24121 gatagacaaa aacgaataaa atgacgaaaa ttaagcagac aaccagtgct tcgatcttac
    24181 gaaacccgac ttttgtcagt agtaagagaa cgaaaacatc taatacagta ataaagacgg
    24241 caatcaccaa tggaatatca aaaagtaagt agagggcgat tgctgcacca atgacttctg
    24301 caatatctgt tgccataatg gctaattcag tcaagatcca taaaatgaca cctaatgtct
    24361 tacttgtgcg tgctcggatt gcctgtgcca aatccatttg gctcacgatt cctaattttg
    24421 ctgccatata ctgtaaaagc atagcaatca gactagacat caaaataact gacatcaata
    24481 aatattggaa attttgtcct ccggtgatcg aagtagacca gttacccggg tccatatacc
    24541 caactgctac taacgcacca ggtccagagt aagcaagaag tgttttaaaa aaacttttcc
    24601 cctttggtac atcaactgtt ccatttactt cagctaaaga taaaccattt gtatgttcaa
    24661 taagtcgatg cttatgaaat tgtttattgg attgattatt caagaaaaaa ccacctttcc
    24721 tatttatcta cgattacatt ctacacctca aaaagaaaaa attaaagaaa aagattcggt
    24781 acgcctaatt ttttttcatg gcgcgtcttg ctagatgacg aaagaaattt tgttctcctc
    24841 taaaaaaacc actatttttt gcatgaaact aaagaatacg cctttctttt ttcaataaac
    24901 tttagtaaaa taagtcattg agagcaattc ggaaatagaa aggagagatt tatatgttat
    24961 caacaggtat tgtcgtactt attgctatta ttgctttgct aatcggcgca gtaggtggct
    25021 ttttcctagc gcgtaaatat atgaaagact atttagaaaa aaatcctccc gttaatgagg
    25081 acatgttgcg atcaatgatg atgtcaatgg ggcaaaaacc atctgagaag aaaattcgcc
    25141 agatgatgca gcaaatgaag aaccaaggga aaaaataggt tctgacccaa cgacttttta
    25201 cgggctgatt ttttcagtcc gttttttatt tcgtaatttg ttagactatt cagattattc
    25261 agcaaaaaag acaagcacag acaatcctag gctcttgata aagacaaagg aggagtaaaa
    25321 tgtcgatttt tagaaaatta ggctggtttt ttcgtcagga aaagaaacat tatttaattg
    25381 gggttttctc gctattcgca gtggccttgg tacagttagt tcctccaaaa gtgattggga
    25441 tcatcattga tgagatcgcc gataaaaatt tatcaataaa gatcattttc gggtggatct
    25501 tcctattgat tatcgctgct tttttacaat atctttttcg ttatatctgg cggacgaata
    25561 tttggggaag tgcagctcga ttggagaaag aacttcggca gcagttattc aaacatttta
    25621 cagaaatgga caatctcttt taccaaaaat atcggactgg ggatttgatg gctcacgcaa
    25681 cgaatgattt gaatgcgatc cagaatgtag caggagctgg tatcttgacc tttgcagatt
    25741 cagtgattac gggcggaaca acgatcattg cgatgatttt gtttattgat tggcgcttaa
    25801 cactgattgc gctgattcct ctgcctttgc tggccgtcac ttcacgagtg ctggggtcaa
    25861 aactccatga tgcttttcgt gattcgcaag aagctttttc tagtatcaat gataagacac
    25921 aagaaagtat cactgggatc aaagtcatta aaacatttgg acaagaaaaa gaagatctag
    25981 cggattttga agaaaagatt gaagatgcaa ttattaaaaa caaacgagtc aattttttag
    26041 actccttatt tgatccattc attaccttga ttatcggtct gtcttatgtt gcaacaatta
    26101 ttttaggtgg aaagtatgtc ttggaaggca cgatctcgat cgggcagatg gtctcattta
    26161 ttagttatat cggtatgctt gtttggccga tgtttgcgat tggtcgattg ttcaacgtat
    26221 tggaaagagg aaatgcttct tatgatcgcg tcaatgaact tttgcatgaa aagacgcata
    26281 tcgtggaaca actgggtgca gtgcagaccc cggcaaaagg cgagctgaat gtagctattt
    26341 cctcttttaa ttatccgaaa gaagaatcac cagcattaga acaaatcaag tttgcgatcc
    26401 aagaaggaga aacattaggt attgttggga aaactggtgc gggaaaaacg acgatcatca
    26461 aattactgat gcgtgaatac gatgattatc aagggaaaat ccaatttggc ggacgttcga
    26521 tcaaagaata ttcattgaat gctttattag gatcaatcgg ctatgtgcct caggatcact
    26581 tcttgttttc gatgaccatt cgagataata tccgtttttc taatccaaaa ctttcagaag
    26641 aagaagtaga gcatgcggct tctttagcat ttatcaacaa tgaaatcaag gaattaccaa
    26701 aaggttatga taccttagtt ggagaacgtg gagtctcttt atctggcgga cagaaacaaa
    26761 ggatttcgat tgctcgagca ttgatcaaaa atcctgaatt attaatatta gatgatgcac
    26821 tctctgcagt agatgccaaa acagaagaag ctatcttgca taatttaaaa gaaataagaa
    26881 aagagaaaac aactatcatt gcagctcacc gtttaagtag cgtgatgcat gcaaaagaaa
    26941 tcattgttgt cgatcaaggg aaaatcattg aacgaggaac ccatcatgag ctgcttgcac
    27001 taggcggctg gtatgaacaa atgtgggaga aacagcaatt agaagcgaag atagaaggag
    27061 gcgaggcctg atggatgaaa attatcaatc ggaatggaca aaatcgattc cacttaaaga
    27121 acagaaaaag atcatcaaac gactgatgaa gttcgccaaa ccttttagaa gaacgttcat
    27181 cgttgctatc ttatttgcgt ttgctttgtc tgtgatcaat gtcctattgc caaggatcat
    27241 ccaaactttt atggatgatc atctggctaa acagtcggct acgacacaag ttattttgtt
    27301 ttttgctggt atgtacttat ttggagtcat agtaaaaagt atcatttggt ttttccaatg
    27361 gtatttgtat tccatggcct cattgaagac ctatcagtat attagggtaa aattatttga
    27421 aaagctgcat acactaggta tgcgttattt tgatcagaca ccggcaggat caaccgtttc
    27481 tcgagtgacg aatgacacag aaacgctatt tgaattttgg tacgtctttt tgatggtatt
    27541 gactgggatc tttgcagtcg tttcttcatt ccttgccatg tttcaaatca atggagaaat
    27601 tgccttgtgg actttggtgt ttttgccgat tttaggaatc gtcatctggt attaccagaa
    27661 attcagttct cgtatttacc gaaatatgcg ggaaaaatta agtcagctga acacgaaatt
    27721 gaatgaatcg atttccggca tgcgtattat ccagcagttt cgccaagaag ctcgtttagc
    27781 taaagaattt gaacaagtga acaatgagta tttagacacg cgttatgcga tgatcaaaac
    27841 gaattcgatt ctgttaagtc cgatcatcaa ttttctatat gcattggcga ttgcgttggt
    27901 gttgactttg tttgggatcg atgcgttgca ttctcccgta gaagttggac tgatctatgc
    27961 atttacgacc tatgtccagg gattttttaa tccaatgtct caaatgatgg actttttgag
    28021 catcttcaca gatgggatgg ttgctggtag tcgtatcttg aagattatgg atacggaaga
    28081 atatgcacca caacaaaacc ctggagcaga tcaaatgatt acttcaggta agattgagtt
    28141 tcgcaatgta agcttttctt atgatggaga gcacaatgta ttaaacaata tctcttttgt
    28201 tgctaatccg ggagagaccg ttgcattagt gggacacacc ggaagcggaa aaagctcgat
    28261 catcaacgta ttgatgcggt tttacgaatt ttatgaaggt cagatattga ttgatgatca
    28321 tgacattcga gaatatccaa tggaagagtt acgtaagaaa atggggctgg tcttgcaaga
    28381 tgctttcttg ttttatgggg atatcgcagg aaatattcgg atgatgaatc cagctatcac
    28441 agacgaacag atacgccatg cggctgagtt tgttcaagca aacaaattca tcgatgaatt
    28501 accaaaagga tatcactcaa aagtgatcga acatggtgcc agctattcta gtggacaacg
    28561 acagctgatc acttttgcgc gaacaatcgt gactgatcct aaaattctag tattggatga
    28621 agcaacagca aatatcgata ctgaaacaga aggattgatc caagaaggat tggcaaaaat
    28681 gcgtcaagga cggacaacga ttgcgattgc ccatcggctg tcaacgattc gagatgcaga
    28741 attgatttta gttttagaca aaggtcatat agtagaacgc gggaatcatg aaacacttct
    28801 agcgcaaaat ggtttgtatg cagatatgta caaactgcaa agcagtggta tataaaagat
    28861 gttgtgacag tagaattttg acctaagcac tagagtgaaa ataaaacata gcttctcata
    28921 tttttcatct gtgagagtta gtgtcgtagt caccagattt aaaaaaagat tcgacagcta
    28981 gtcagaagtg cctaatagcc actaagaaag cgttgacaaa agcatttgct ttcgataata
    29041 taaagaaaac gatacaactt ggatagtgat tgcctgatgc aagctaaacc tgatgagtct
    29101 cccatggttt catggacggc tttttgggtt tttctttttg ttttggatac agccctatac
    29161 atcatggaaa ggagcaaaac atgctgattg agaaagtact gaataacaac gtcgtaataa
    29221 ccagggatga aagaaatcag gaaatgatcg tgatgggaaa aggtttggca ttcaaaaaga
    29281 aagtgggtga cccgattttt gactcatgca tcgacaaaaa atttcagttg acaaatgacg
    29341 ggctttcaca acagttccaa gaattggcac aagcgattcc tcttgagtac ttagaaattt
    29401 cttgtgaaat cattgaatat acaaaagagg ctctacaagt ccagttgaat gattctgtgt
    29461 atattgcatt gacggatcat ttgtatacgg ctatcgaacg tcacaaaatg ggaatatttg
    29521 taccaaatgt attgctgtat gatatcaaac aactatttcc taaagagtat gccatcggga
    29581 aaaaaacagt gaagaaaatc tcggataaat accacatcaa gttatcagaa gatgaagccg
    29641 gatttatcgc attgcattta gtcaatgcac aaggcattca aagccagcaa gacaatacct
    29701 acctgtttac tataacaata caggatattc taaatatcgt ccgctattac tataagatta
    29761 catttgatga agattcagtc catttttttc gattcgtcac acacttacgt ttttttctga
    29821 cacgcgtcct agatggagaa aaatcagaag tgggagaagt ttctgaggaa ttgtttcagg
    29881 ttattcaaaa aaaatataag gatacgtttg gttgcgttga gaaaatagca gactttttag
    29941 ccgagacgtt caactaccag atgtccacgg acgaaaaaat gtatttaacg atccatactg
    30001 cgcgtttagt gcatgagaat cgttgtaaat aaaaaaagga ggataaatta acaagaacgt
    30061 atgaaataag ggtagtgaca ttcagttggc taaaccttcc actaataaat tggagggaat
    30121 agaaatggga aaataccaag aattagcgag aaaaatcgta gaaaatgtcg gcgggaagga
    30181 aaatatcaat ggtttgacac attgtatcac aaggcttcgt ttcaaattaa aaaacgaaga
    30241 aaaagcaaat gatgagatct tgaaaaatat ggacggtatt gtaacggtaa tgcgagcggg
    30301 agggcagtat caagttgtca ttgggaatca tgtgccagtc gtgtttgaag aggttataaa
    30361 agcaggaaac cttacttttg atgaggctgt ttcaacagaa aaaatgagac cgtttgatat
    30421 gcttattgat ggaatatcgg gctgtttcca gccattcctt gctatcctag ctgctggcgg
    30481 gatgattcgt ggactgacag cattccttgt cttcttaggc gcgtttgacc gcggttctgg
    30541 cacatttgtt atgtttgata atatcggtga ttctgtattc caatttatgc ctgttattat
    30601 cggtttaaca gctgcccgta aatttaaagt aaatgaattc gtagggatgt tgcttggcgc
    30661 agcattaatg aatcctagct tgtcgctgga agctttgagt ggtgcagcag aagcaccact
    30721 aacgaccata ttttctggca ctatttttga agcacctatt tatcaaacag tattcggcat
    30781 tccctggatt gcgcgtaact acgcttcttc tgtcattccc attatcttta ttgtgttgtt
    30841 agctagccaa attcagaaac caattaaaaa gttagtacct gagatgatcg cgaatttctt
    30901 cgttccattc ttcaccgttc ttattacaat gcctttaggt tttttactcg ttggaccagt
    30961 attcacattc gcaactgata ttttaatggc tggatttgaa acacttttag cactttcgcc
    31021 agttatttat ggagcaatcg taggcttctt ctggcaaatt ctggtgatgt ttggtctcca
    31081 ttgggcaatc gtaccaatgg gattgatgca attttctgtg aatggttggc aaaatattat
    31141 gacacctgtt gcagtagtgt cttttggaca aactgcagca cttaccgcac tctactttaa
    31201 gcttagaaat ccaaaagaca aagcaattgc aataccagcc attgtttctg gtattgtggg
    31261 gattacagag ccagctattt atgggtttac attaccacgt aaaaaaatat ttatttttac
    31321 ctgtgtcggt ggtgcgttag ggggcgcgta tagtgggtta atgaatttaa cttcttggaa
    31381 tcaaggtgga ctaggcatct ttacaattcc caactatatt agaccagatg gtgatttaac
    31441 agatgtgatt aatgtattga ttgggatcgc cattgcgatg gtggtatcct ttacgctgac
    31501 ttttttcttt tggaaggatg aagcaggtga aacagatgat atacagaaaa aatcaggtaa
    31561 agaaattgta aaaacaccga tacaaggaca aatcgctcca ctaaatgcag caaaggatgc
    31621 agcttttgca caaggaacac taggtcgagg aatcttgatc taccctgaaa aaggagaagt
    31681 acgtgcccct tttgacggaa cagtcatgac cctgttcccg acgaaacagg caattggaat
    31741 ggtttctgaa acagggctgg aattgctgat tcatgtcgga ttggatactg tgcagttgga
    31801 agggaaatat tttgaaagct tagtgcagca aggataaaga gtgaaacaag gcgatctgtt
    31861 gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac agtttagaaa cacctgtgat
    31921 cgtcaccaat tacacggatt atttggatat tttggaaaac caaggggaga cggtcgggcc
    31981 agacgatgca cttatcaccg cactgacctg aacggttagg tgaagaatgg actaggctta
    32041 actattcttt ttattccact tctttatttt caaaattgaa aggctctttc atgatggctg
    32101 agctgatagg gcgtttatcg aatttttgac cgacagaaat tagatggact aaattctgtt
    32161 gataattttc tgcaaaaaca caactatata acacgtctag aagataacaa attgatgtat
    32221 tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg tgtcgtaagt cgcaaagcgg
    32281 ctgtactcat tgcagatagc gttgaatcac cgatactggt caaagctaaa attggaacat
    32341 gattttgttt caatatttct gcggtacgaa ggatcacatt gttttcaccc gtataagaaa
    32401 tcagaatccc gcacatatca ctcgtgcaat tgtacgcttc atagaatgat tcaccaaaag
    32461 tggtgacaat cgtgacatcc attttgatgc gcttcatttt cagcatgaaa tcttgagaaa
    32521 tcaaagtatt cgagttactg gcgaaaatca cgattttttt tgcttgaagc attagttgtt
    32581 tggctttttg cagttggtca tgatgaatga gagaaagtgt atcatgaatc gtcatctgtt
    32641 ctaaagaagc cattttattt gcgatcgtca taataccgtc tgtggtggaa aaaggaagat
    32701 tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt ttcagctaaa aaagcattac
    32761 gaaaactgac ccagccatca aaacccattt tttttgctac acggatgagt gtagaggggt
    32821 ggacataaca tcgctcagca atcgcttgga tggtcatttc gccaagtgct ggagaatcgc
    32881 ttaagagaaa ctggatcact tcattttctg aaggagaaaa ggagatttct ttgatttttt
    32941 cacgtaataa catgtagata ccttcttctt atttttttac gtaaattata acttttttga
    33001 aaat
//
LOCUS       DAHUWM010000007        30688 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHUWM010000007 DAHUWM010000000
VERSION     DAHUWM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.007"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     regulatory      <1..69
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            185..766
                     /locus_tag="NK788_000204"
     CDS             185..766
                     /locus_tag="NK788_000204"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCH6768583.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            769..2076
                     /locus_tag="NK788_000205"
     CDS             769..2076
                     /locus_tag="NK788_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCH6768584.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2369..2857
                     /gene="purE"
                     /locus_tag="NK788_000206"
     CDS             2369..2857
                     /gene="purE"
                     /locus_tag="NK788_000206"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCH6768585.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2850..3974
                     /gene="purK"
                     /locus_tag="NK788_000207"
     CDS             2850..3974
                     /gene="purK"
                     /locus_tag="NK788_000207"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCH6768586.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            3987..5282
                     /gene="purB"
                     /locus_tag="NK788_000208"
     CDS             3987..5282
                     /gene="purB"
                     /locus_tag="NK788_000208"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCH6768587.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5462..5839)
                     /locus_tag="NK788_000209"
     CDS             complement(5462..5839)
                     /locus_tag="NK788_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768588.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6095..6823
                     /locus_tag="NK788_000210"
     CDS             6095..6823
                     /locus_tag="NK788_000210"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCH6768589.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6823..7077
                     /gene="purS"
                     /locus_tag="NK788_000211"
     CDS             6823..7077
                     /gene="purS"
                     /locus_tag="NK788_000211"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCH6768590.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7079..7750
                     /gene="purQ"
                     /locus_tag="NK788_000212"
     CDS             7079..7750
                     /gene="purQ"
                     /locus_tag="NK788_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCH6768591.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7751..9973
                     /gene="purL"
                     /locus_tag="NK788_000213"
     CDS             7751..9973
                     /gene="purL"
                     /locus_tag="NK788_000213"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCH6768592.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            9958..11397
                     /locus_tag="NK788_000214"
     CDS             9958..11397
                     /locus_tag="NK788_000214"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCH6768593.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11420..12472
                     /locus_tag="NK788_000215"
     CDS             11420..12472
                     /locus_tag="NK788_000215"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCH6768594.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            12469..13047
                     /gene="purN"
                     /locus_tag="NK788_000216"
     CDS             12469..13047
                     /gene="purN"
                     /locus_tag="NK788_000216"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCH6768595.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13044..14579
                     /gene="purH"
                     /locus_tag="NK788_000217"
     CDS             13044..14579
                     /gene="purH"
                     /locus_tag="NK788_000217"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCH6768596.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14595..15869
                     /gene="purD"
                     /locus_tag="NK788_000218"
     CDS             14595..15869
                     /gene="purD"
                     /locus_tag="NK788_000218"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCH6768597.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            15991..16905
                     /locus_tag="NK788_000219"
     CDS             15991..16905
                     /locus_tag="NK788_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6768598.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17038..18603)
                     /locus_tag="NK788_000220"
     CDS             complement(17038..18603)
                     /locus_tag="NK788_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCH6768599.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18596..20047)
                     /locus_tag="NK788_000221"
     CDS             complement(18596..20047)
                     /locus_tag="NK788_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6768600.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20061..21077)
                     /locus_tag="NK788_000222"
     CDS             complement(20061..21077)
                     /locus_tag="NK788_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6768601.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21297..22385
                     /locus_tag="NK788_000223"
     CDS             21297..22385
                     /locus_tag="NK788_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCH6768602.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22439..23965
                     /locus_tag="NK788_000224"
     CDS             22439..23965
                     /locus_tag="NK788_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCH6768603.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            23967..25172
                     /locus_tag="NK788_000225"
     CDS             23967..25172
                     /locus_tag="NK788_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6768604.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25429..26406
                     /gene="guaC"
                     /locus_tag="NK788_000226"
     CDS             25429..26406
                     /gene="guaC"
                     /locus_tag="NK788_000226"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCH6768605.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26670..28094
                     /locus_tag="NK788_000227"
     CDS             26670..28094
                     /locus_tag="NK788_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCH6768606.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28555..29664
                     /gene="pdhA"
                     /locus_tag="NK788_000228"
     CDS             28555..29664
                     /gene="pdhA"
                     /locus_tag="NK788_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCH6768607.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29667..30644
                     /locus_tag="NK788_000229"
     CDS             29667..30644
                     /locus_tag="NK788_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCH6768608.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9859 a         5229 c         6946 g         8654 t
ORIGIN      
        1 gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga
       61 ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa
      121 catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa
      181 taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt
      241 attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg
      301 aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc
      361 ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc
      421 tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt
      481 tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa
      541 agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt
      601 atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca
      661 agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc
      721 ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa
      781 acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga
      841 gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact
      901 tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt
      961 aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct
     1021 ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct
     1081 tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct
     1141 cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc
     1201 gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta
     1261 cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt
     1321 cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga
     1381 atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat
     1441 tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc
     1501 agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt
     1561 ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt
     1621 attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg
     1681 ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta
     1741 ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg
     1801 ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc
     1861 agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat
     1921 gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc
     1981 atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa
     2041 gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa
     2101 tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc
     2161 gtttgtttga gctaataccg agggtcagct gattgaacac cgtttatcca taaaaaggaa
     2221 tgtgacctaa cttttgtcac attccttttt ttcatcttta ataaacacga acaataaaat
     2281 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
     2341 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
     2401 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
     2461 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
     2521 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
     2581 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
     2641 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
     2701 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
     2761 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
     2821 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
     2881 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
     2941 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
     3001 ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
     3061 atgtcattac ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg attttaccca
     3121 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
     3181 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
     3241 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
     3301 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
     3361 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
     3421 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
     3481 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
     3541 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
     3601 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
     3661 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
     3721 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
     3781 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
     3841 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
     3901 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
     3961 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
     4021 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
     4081 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
     4141 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
     4201 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
     4261 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
     4321 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
     4381 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
     4441 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
     4501 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
     4561 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atctctactc
     4621 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
     4681 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
     4741 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
     4801 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
     4861 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
     4921 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
     4981 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
     5041 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
     5101 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
     5161 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
     5221 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
     5281 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
     5341 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
     5401 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
     5461 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
     5521 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
     5581 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
     5641 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
     5701 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
     5761 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
     5821 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
     5881 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
     5941 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
     6001 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
     6061 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
     6121 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
     6181 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
     6241 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
     6301 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     6361 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     6421 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     6481 tccatttatc aatgaagagc atatctattt cttacaactt gcaaaaccgg aagaaatcag
     6541 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     6601 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     6661 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     6721 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     6781 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     6841 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     6901 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     6961 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     7021 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     7081 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     7141 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     7201 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     7261 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     7321 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     7381 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     7441 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     7501 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     7561 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     7621 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     7681 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     7741 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     7801 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     7861 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     7921 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     7981 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     8041 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     8101 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     8161 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     8221 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     8281 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     8341 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     8401 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     8461 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     8521 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     8581 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     8641 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     8701 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     8761 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     8821 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     8881 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     8941 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     9001 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     9061 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     9121 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     9181 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     9241 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     9301 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     9361 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     9421 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     9481 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     9541 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     9601 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     9661 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     9721 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     9781 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     9841 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     9901 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     9961 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    10021 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    10081 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    10141 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    10201 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    10261 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    10321 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    10381 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    10441 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    10501 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    10561 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    10621 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    10681 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    10741 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    10801 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    10861 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    10921 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    10981 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    11041 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    11101 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    11161 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    11221 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    11281 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    11341 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    11401 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    11461 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    11521 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    11581 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    11641 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    11701 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    11761 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    11821 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    11881 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    11941 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    12001 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    12061 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    12121 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    12181 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    12241 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    12301 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    12361 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    12421 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    12481 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    12541 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    12601 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    12661 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    12721 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    12781 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    12841 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    12901 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    12961 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    13021 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    13081 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    13141 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    13201 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    13261 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    13321 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    13381 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    13441 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    13501 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    13561 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    13621 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    13681 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    13741 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    13801 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    13861 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    13921 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    13981 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    14041 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    14101 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    14161 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    14221 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
    14281 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    14341 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    14401 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    14461 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    14521 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    14581 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    14641 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    14701 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    14761 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    14821 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    14881 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    14941 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    15001 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    15061 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    15121 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    15181 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    15241 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    15301 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    15361 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    15421 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    15481 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    15541 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    15601 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    15661 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    15721 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    15781 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    15841 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    15901 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    15961 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    16021 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    16081 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    16141 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    16201 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    16261 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    16321 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    16381 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    16441 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    16501 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    16561 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    16621 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    16681 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    16741 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    16801 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    16861 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    16921 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    16981 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    17041 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    17101 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    17161 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    17221 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    17281 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    17341 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    17401 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    17461 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    17521 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    17581 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    17641 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    17701 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    17761 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    17821 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    17881 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    17941 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    18001 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    18061 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    18121 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    18181 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    18241 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    18301 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    18361 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    18421 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    18481 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    18541 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    18601 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    18661 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    18721 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    18781 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    18841 ctttgaattg gatcatctca tcaattttgc atgcggtaat acgaataaat cctttgtgac
    18901 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    18961 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    19021 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    19081 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    19141 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    19201 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    19261 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    19321 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    19381 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    19441 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    19501 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    19561 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    19621 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    19681 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    19741 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    19801 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    19861 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    19921 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    19981 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    20041 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    20101 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    20161 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    20221 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    20281 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    20341 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    20401 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    20461 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    20521 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    20581 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    20641 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    20701 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    20761 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    20821 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    20881 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    20941 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    21001 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    21061 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    21121 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    21181 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    21241 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    21301 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    21361 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    21421 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    21481 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    21541 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    21601 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    21661 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    21721 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    21781 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    21841 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    21901 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    21961 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    22021 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    22081 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    22141 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    22201 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    22261 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    22321 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    22381 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    22441 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    22501 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    22561 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    22621 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    22681 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    22741 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    22801 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    22861 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    22921 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    22981 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    23041 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    23101 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg ggatggtggg
    23161 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    23221 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    23281 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    23341 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    23401 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    23461 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    23521 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    23581 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    23641 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    23701 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    23761 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    23821 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    23881 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    23941 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    24001 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    24061 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    24121 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    24181 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    24241 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    24301 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    24361 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    24421 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    24481 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    24541 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    24601 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    24661 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    24721 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    24781 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    24841 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    24901 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    24961 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    25021 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    25081 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    25141 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    25201 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    25261 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    25321 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    25381 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    25441 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    25501 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    25561 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    25621 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    25681 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    25741 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    25801 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    25861 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    25921 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    25981 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    26041 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    26101 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    26161 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    26221 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    26281 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    26341 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    26401 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    26461 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    26521 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    26581 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    26641 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    26701 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    26761 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    26821 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    26881 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    26941 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    27001 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    27061 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    27121 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    27181 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    27241 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    27301 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    27361 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    27421 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    27481 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    27541 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    27601 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    27661 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    27721 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    27781 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    27841 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    27901 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    27961 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    28021 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    28081 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    28141 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    28201 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    28261 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    28321 atgcagatga aaaataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    28381 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    28441 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    28501 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    28561 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    28621 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    28681 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    28741 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    28801 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    28861 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    28921 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    28981 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    29041 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    29101 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    29161 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    29221 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    29281 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    29341 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    29401 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    29461 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    29521 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    29581 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    29641 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    29701 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    29761 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    29821 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    29881 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    29941 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    30001 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    30061 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    30121 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    30181 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    30241 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    30301 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    30361 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    30421 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    30481 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    30541 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    30601 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    30661 acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAHUWM010000008        29259 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHUWM010000008 DAHUWM010000000
VERSION     DAHUWM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.008"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(144..368)
                     /locus_tag="NK788_000230"
     CDS             complement(144..368)
                     /locus_tag="NK788_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768609.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(388..810)
                     /locus_tag="NK788_000231"
     CDS             complement(388..810)
                     /locus_tag="NK788_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCH6768610.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(954..1664)
                     /locus_tag="NK788_000232"
     CDS             complement(954..1664)
                     /locus_tag="NK788_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768611.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1682..2179)
                     /locus_tag="NK788_000233"
     CDS             complement(1682..2179)
                     /locus_tag="NK788_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6768612.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2216..2668)
                     /locus_tag="NK788_000234"
     CDS             complement(2216..2668)
                     /locus_tag="NK788_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6768613.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2803..3408)
                     /locus_tag="NK788_000235"
     CDS             complement(2803..3408)
                     /locus_tag="NK788_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCH6768614.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3858..4478)
                     /locus_tag="NK788_000236"
     CDS             complement(3858..4478)
                     /locus_tag="NK788_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6768615.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4480..5349)
                     /locus_tag="NK788_000237"
     CDS             complement(4480..5349)
                     /locus_tag="NK788_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCH6768616.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5372..5779)
                     /locus_tag="NK788_000238"
     CDS             complement(5372..5779)
                     /locus_tag="NK788_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCH6768617.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5798..7087)
                     /locus_tag="NK788_000239"
     CDS             complement(5798..7087)
                     /locus_tag="NK788_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6768618.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(7114..7416)
                     /locus_tag="NK788_000240"
     CDS             complement(7114..7416)
                     /locus_tag="NK788_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6768619.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(7441..9534)
                     /locus_tag="NK788_000241"
     CDS             complement(7441..9534)
                     /locus_tag="NK788_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6768620.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(10023..10787)
                     /locus_tag="NK788_000242"
     CDS             complement(10023..10787)
                     /locus_tag="NK788_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCH6768621.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(10898..11767)
                     /gene="mdcB"
                     /locus_tag="NK788_000243"
     CDS             complement(10898..11767)
                     /gene="mdcB"
                     /locus_tag="NK788_000243"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCH6768622.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(11792..12868)
                     /locus_tag="NK788_000244"
     CDS             complement(11792..12868)
                     /locus_tag="NK788_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCH6768623.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(12892..13485)
                     /locus_tag="NK788_000245"
     CDS             complement(12892..13485)
                     /locus_tag="NK788_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCH6768624.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(13489..15165)
                     /gene="mdcD"
                     /locus_tag="NK788_000246"
     CDS             complement(13489..15165)
                     /gene="mdcD"
                     /locus_tag="NK788_000246"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCH6768625.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(15155..15454)
                     /locus_tag="NK788_000247"
     CDS             complement(15155..15454)
                     /locus_tag="NK788_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCH6768626.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(15556..17202)
                     /gene="mdcA"
                     /locus_tag="NK788_000248"
     CDS             complement(15556..17202)
                     /gene="mdcA"
                     /locus_tag="NK788_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCH6768627.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(17209..18120)
                     /locus_tag="NK788_000249"
     CDS             complement(17209..18120)
                     /locus_tag="NK788_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCH6768628.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            18240..19097
                     /locus_tag="NK788_000250"
     CDS             18240..19097
                     /locus_tag="NK788_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6768629.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(19284..19646)
                     /locus_tag="NK788_000251"
     CDS             complement(19284..19646)
                     /locus_tag="NK788_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCH6768630.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            19992..21431
                     /locus_tag="NK788_000252"
     CDS             19992..21431
                     /locus_tag="NK788_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768631.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(21438..23990)
                     /locus_tag="NK788_000253"
     CDS             complement(21438..23990)
                     /locus_tag="NK788_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCH6768632.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(24104..25435)
                     /locus_tag="NK788_000254"
     CDS             complement(24104..25435)
                     /locus_tag="NK788_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6768633.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(25401..26879)
                     /locus_tag="NK788_000255"
     CDS             complement(25401..26879)
                     /locus_tag="NK788_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6768634.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(27184..29019)
                     /gene="tdc"
                     /locus_tag="NK788_000256"
     CDS             complement(27184..29019)
                     /gene="tdc"
                     /locus_tag="NK788_000256"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCH6768635.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         8415 a         6217 c         4971 g         9656 t
ORIGIN      
        1 taaacaaaaa attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta
       61 tctcaaagtt catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag
      121 tttcttcatt ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt
      181 tcttctttga tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct
      241 gtatcgacca tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct
      301 cgaagcttct cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt
      361 agatccactg tttttcctcc tttttattca atcagacgac tcagcttttc ctataacaaa
      421 ataattgcct tcagggtcag aaaaattgaa agtcaagatg ccattgttat ctgtgatttc
      481 tcctggttct tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga
      541 aggcggtggt cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc
      601 taattctaat tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat
      661 tgtttcagaa atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc
      721 attgacataa agcatcacac gtgttttttg tatcatcatt tgacccttct ttctatttga
      781 ttttctttcc tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt
      841 tgatactatt caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt
      901 ttttacaaaa aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt cttttataat
      961 tctgcttctg attcatcttt ggttagtgtg tgtctaacac tagttacgcc aaatgtttga
     1021 tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg
     1081 atggattcaa tgaccagcac cgctgcttgt gtttcttccg tcagcattgt tctttgggct
     1141 tcacgccagt tgagacagcg acagatcgct ccttgttcat catagtagat gatttcttcc
     1201 ggaagtgccg gagcatcgga atctgcacct aaaggcagga atgattcgcc accttgggct
     1261 tttcctaaat gcagatttcc tgcgatcttt tcgatatctt ctccgccgca aggtacgcca
     1321 aaagacaagg aaatactatt atagatatct acaagcggat tgattgggaa aaattcccgt
     1381 tcttgatttg cacgtttcag taaagcttca atagaagaac gtgcaccttt tttagtcttg
     1441 aatgtggtaa atgcctgacg ccactgagca atgacttcgt tttggctgaa tgtttcttct
     1501 gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata attgatgata cgtttcttca
     1561 ctttcttttg ttacgtggtt atcgatacca tttacggata agatttcgat ggttgcttca
     1621 ggaaatactt cccagaaatt ggtatccact attacttttt tcatctgttc acacctcttg
     1681 attagaataa ttgcttgatg aatagatagt ctgtttgggg atcatctccc atataaaaaa
     1741 catgttcgct gtatttgata aatccttttt tacgataaaa agcgagcgca acatcgttgt
     1801 gttcccaaac accgagccaa atggctgttt tttgcttttc ttttgctttt tcaatagctg
     1861 tatctagtaa aaatgtaccg ataccttgat gtttaaattt ttttcgtaca tagattcgct
     1921 cgatttctaa aaaatgatca gccatttcct cagattgact cgttcctgta tttagtttca
     1981 ggtaacccgc aggctcttga tcgatactca ctaggaaaaa atcggtttct ttatctttta
     2041 gctcttctgt aagtttttct agtgcataag cttgtgtcaa gtaatcagct aaatgctccg
     2101 gtgaattatc ttttccaaat gtatctgtaa atgtttcgat actaatttgt tgtagtaagt
     2161 ctacatctga aggcgtcatt tttcgaattt cgtatttatt cacggcttct tcctttcaat
     2221 agatcctttt attgcctttt ttgacaaaat tccaatcttc gctaacattc ttgcatactt
     2281 tatttaaaag atattccaat tgttttgctt cttcgtcaga aagaccattt aatgcgactg
     2341 cattggagta gttgttttct cgaatgatga aaggatagat ttctgctcct tttttagtcg
     2401 gatagagttt cttgattttt ttattttctt tatcttccaa acgttcgatc atcccattgg
     2461 attctaattt cttgattgct cgggcagtcg ttgtacgatc tactttgatc atttctgcta
     2521 gtttttcttg gatgattcca ggattttcgc aaatacgtac taaataaaga tactgtcctc
     2581 ttgttaaatc gacttctttg aattcgatat tgctaataga atctagcgct cgggcaatca
     2641 ttccgatgtc tcgaaggatt tcagccattt catcccacct ttattttgtt tgtaagacta
     2701 gtttacctct ttttgttgca aatgcaacta ttttcaataa aaaaatcaag ttctttttta
     2761 ataactagta aagtatattc ttttcttctt ttacgaaaaa gctcaatcac aatagatctg
     2821 gtttttgttt tctaaaataa gtaactgttt cttcgttgtt cgcttttctg atagctttgg
     2881 caactcttct atactaaaaa atcccatgtt ggaagtttca ttattctcaa taaacttggc
     2941 ctcttcaccg tggatcgcac aagcaaaaac aagtttataa tactgaaaaa gctgtggaat
     3001 atctttttgt ttattcgtat caaaaactgc tcgcaactga gtagtttcta ctgttaatcc
     3061 tgtttcttca tagacttctt tacgcacatt ttcttcagga gacaatccta tttcagcaaa
     3121 tccaccagga agtgaccatt ctttcgtccg tttatcttcg attagtagga ccttgccatc
     3181 ttttttgata taggcacgaa catctacttt tggtgttgga tgtccttctc caatcgttgt
     3241 gagcgctttt atttcttcga aagtttcttt tcctatttca ctgatcagtt ccaatgaaat
     3301 cgtccggatc tcctcaaaac gctctttatc aaatacatct tttccataat atagaccagc
     3361 ttgagaaagt gcgagtagtc ttttgtattg agccaacttc ttgttcattc tcttcccttc
     3421 ttttttagca aatgtatgac tatgtaatca agtatagaag actggtttaa ttacggcaaa
     3481 taaatataat gaaatctgca ataaataaaa gaatgttaaa aaactcgaga acaaaagaca
     3541 ttcgacttct ctggggctgt gaataaatgg cgttcaaaag cttgcatctg cggtaataag
     3601 ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg ctgttcttat taattgaggc
     3661 aggacagctt tttcacaacc tccccactaa acggtgttct cctaccaact cctcggtcac
     3721 aagtcacaat attcatcaaa catgagaagc tgtttttttg aaattgctcc ttgtgactcg
     3781 gagctgaacg acaggctcac gacctctcgg taaacagcgt tcaaaagcca atttttcgat
     3841 attaagccat ttctttgtta cttattgtca ttgatttctt tttctaattt atccaatagt
     3901 ggagtgtaat ccacttgagt atagtctgtt acatcaaatt catatagtgt tcctaactgg
     3961 aatttgtcat attttcggtc ggcgatgatc tttttgaatg cttctttcgt aatatgattg
     4021 gtccctttcg ttcgatcttc caatacgtca ccatagtgat aatgatccat gatatagctc
     4081 aaatgacgat ctgattcacg atcacgtttg taccgacgtt gacataacac ttcaaagtct
     4141 gctgttaggc gaatcgtgat cacctgatat ccgtatctat ctgctaattc ttggagtcgt
     4201 gctttttgct tatcactaaa aggatactcg gacacaatca ctttctttcc tgcttccata
     4261 tacaaatcaa gtgctttgta atagaaagga taaactttcg tcctttcttg ttccgcccgt
     4321 tcttccaaat tgttaaaccc gatggattct gcatagtatt ccttgatttc atcgagcgtg
     4381 atggcataca tatctggaaa tcgttcccgt agtttattca taagatatgt ttttcctgta
     4441 ccaggactac cagccagaag aatgaaatac tttttcatct catttatttc ctaagttaat
     4501 tggactttcc aagttcataa acagtccaat cttatgcttg acaatttctt ttactgcttc
     4561 atttgctgga atcacattat ggcgaagaga cttgttgatt tctgtttcac taaaggtttc
     4621 tttagctact ttcgtccagt tgactaaaag ctccgttcca acattgaatt tgcggatccc
     4681 gttattgatc aattcaggat aatcttcttc tttcacacct gttcctccat ggataactag
     4741 ggggacatca actactgcat cgatttcttt cagcaaggga atgtttactt ctgtttttga
     4801 tttgaattgt ccatgattcg taccgatagc aatagctaat gcatccacgc ctgtagcttc
     4861 tacaaatttc actgcgtctt ccggtcttgt atataccttg tcattttcag cgacatggat
     4921 tccttcttct gtaccgccga ttgttccaag ttctccttct acggaaacac cgtgcgcatg
     4981 ggcatattct actactgctt ttgtcttcag gatattttct ttgaaaggca gatgggaccc
     5041 atcatacatg acagaagtat atccgctgtc gattgcttct ttgatatcat caaagtctcg
     5101 ggcgtggtct aagtgcaaga ctgcatcaac catttcttct tctgcaatag ctgtcattac
     5161 attgactaac acatgcatgc cgatatattt agcggtatcg acacttgttt gaataatgat
     5221 cggcgaaccc atttcttttg ctgcgcctag catagcagga agcatttcta ggttatgcgt
     5281 attaaaagct ccaactgtaa aattcaactc ttctgctgtt tttgttactt ctttcaatgt
     5341 tgtatacatc ttttattgcc accttatctt tttatttgtt tcctttgatc gttacttgtt
     5401 ttgcaatttg tcgcatctca tccattttgc ccatgtagta atcgatccct tgatcatcac
     5461 cagcacgagc agcatctgca cgcttctggt tatagaggct catcagtttt ttatacccat
     5521 ctccgatcgt ctgtttattt tccaaactct tttctaaata ttggatggct tgatcgttca
     5581 catacaattc agaaaattta agtccaactt gttcataaag tttcgctaat ttatgcttat
     5641 cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg
     5701 cttctggcga tagggctttt tcttcttttg ccagaacttt tttcggttct ttttcttttt
     5761 tcttaaaaaa tccaaacatc attttcccta ctttcaatta aaatgctttc aaaactgggc
     5821 ttaagatcca ggcatataag agtgctgcgg ctactgtatc tacatcggtt gctgtagcat
     5881 tgatgaatcc catcgaatta aagatcgtta ctagcaaagc gggcaataat gtaataaaga
     5941 aaccatgagc aataccgcca atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata
     6001 tccctgctgt ccctccggca aagaaattgg ttagcatacc aggtaaaatc atcgctaagc
     6061 cgaacatggg caaagtaaac atggcaataa tcgtaccaat cgttgtcgta atgaaaccta
     6121 aaatgacagc atttgggcta taaggaaaga aaactggaca atctaaggca gggatcgcat
     6181 caggaactaa tttcatagct atccctcgaa atgccggcac gatttcacct aacagtagac
     6241 gtacaccagc tagcaataca tacacaccca ctacgaattg gatggcttgt aaaaaggcaa
     6301 acatgatata gttttgatct cctgataatt cagaagcgaa tggttcgcct gcaaataaaa
     6361 cagtgatgat atatagcggg accatcacga ccatgacaga caaataagta tcttgcagaa
     6421 attcgaaatg tgtcggcaac ttgatatctt cgatcgactt tttattttcg cctttctcac
     6481 caaaaagctt agcaacaccg gcttcaaata agtacccgat cgtacaaaaa tgacctaaag
     6541 caatatcatt tgatccagtg attttacgaa tgatcggttg cgctactgcc ggcatagcaa
     6601 tggcgaaaca agctccgata aaaccaccga ccaagatcaa tacgatccct cgcagaccgg
     6661 caaaatagcc aaagactgtc gtcatcgttg ccatccataa aattgcttga ccagttaaaa
     6721 aaatgtactt ccattttgtg aaacgagcca aaataatatt gaatacaaaa atggctaaga
     6781 acgtcagtgc aatatctcta cctaaaccca aatcattcat tgcttgacca ttgatagatt
     6841 caatagaagg gatgatccct tgcatatgaa atccttcagt aaagattttc ccgaaataag
     6901 ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac ttgaaatcct aggagtgtct
     6961 ttaacgtgcc agagatgact tgtcctgctg atttcttctg cagtagtaga cccaacatag
     7021 caatcaatgc aatggtgatc gatgcctgag tcaaaatatt ttcgatgata aaattgataa
     7081 cgcccatgtt tttaccctct tttctttttg tatttagata actcccttat cttgcagaac
     7141 tggggtgatt ttttcttcaa tttcattttt tgaaacaata tttttcaaat agatcatcgc
     7201 cgttttagga tcaactgcga acttttcgaa ctgcgaacga aaattttctg ctgtgatgat
     7261 cacatctggt ttcatggaaa ctgcggaaga aatatcacaa tggtccagtt tggcttctac
     7321 tcctaatttg ttcaaaacat cctcagcaga catctgtgct gcaaaactac ttcctaaacc
     7381 tgcaccgcat acgaataaaa tgttcaattt gttcatcttt gttctccccc gtatcattta
     7441 ttatttctca actactgaaa aaagcagttg tttgaaatct tcgatatttg tgcattcaat
     7501 caatcgatcc aacttctctt cgttgttgat caattcaatt agttctttca tgatgttcag
     7561 atgcgagtag gagtctactg ctgccaaaca aaaaataatc tttactggat ccatttcagg
     7621 attaccgaaa tcgattggtt gctcaatcgt agctacgctg actcctaatt tattgacccc
     7681 atcttctggt cgggcatggg ctagtgccat gtgtttgcca atcacgatat acggtccgta
     7741 atcattcact gcacgaatca tcgcttcgac atagcgctct tcgacaatgt tttctttaac
     7801 caatggtcta gcagctagtt caatcgattc ctcccaagta gtggcttttt ctttgatcat
     7861 gatattgctg tccgtcaaaa tatcttttaa cataggctgg atctcccttt tgttgatttc
     7921 taaattgttt gtttcgaata atttttctaa cttgccatag atgattcgat ctactcggcc
     7981 accactttct ttcatgacat ctattatttg agtgaataac tccgtcgatt catgactgtt
     8041 aggctctaac cgctgtagtt cttcatttct tttcaaaaag tcttctataa ccaagatatt
     8101 cgtctcagtt aaaatcggat cgacaactaa aagcggctta tttcgataat tcaattgaaa
     8161 ggtgctgaaa accagattga catccaattt atctactaat tcgatttctt ttgaacttaa
     8221 aactgcagta atctcgatct gaggaaactt ttctttcaga ttctctgcta acagatttcc
     8281 agtagccatc ccatggttac agacaacgac tgatttataa acataattca agtctcgctt
     8341 gattacactg gcgatcgtcg aaaagtggat gacaagaaaa gctatctcgt cctccagtat
     8401 cttgccacca attacttctt cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg
     8461 gaaataattt gttcggatgt tttctttcaa tggattcatg acttggatat gattcttgat
     8521 tcttacaatc agtgcagcca tgtgcttata gagattttcg aataaagctt cttcctttag
     8581 atggaaaggt acgtgtgtct cgttctctac aaattggatc aattggatcg ttagtaattg
     8641 agcttgtacc cactcgattg aattggagat atctctagca ttgaacgtct caatagtgaa
     8701 acgaagataa tttctttcta cttgcgggac attgtccaat tgaaattcct cgatcatgtg
     8761 gtcaataaat gcagaaaagc tactgtcttt tatttctgta tttttccaat taatggcaga
     8821 gataaattca tgtttcctga ttcgctggat ccagatcaat gtaaaaagca ggatttgttt
     8881 tttataaata tcatcttctt gtttcaacaa cttattggaa tagatctcct gtattttttt
     8941 gatttccacc aaaggaaaat aatggagaaa gattttttga gccggtgtta attgtgcatc
     9001 ttgattcata aagaagtcgg tcattcccag attctgattg ataaaatcat agatcatcgt
     9061 acggatagat cgttctgaac ctttataaac cagtccttgc tttccataac tgacgatctc
     9121 tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata tcttcatcta atgtactttt
     9181 agagatttga aattcttctt cttttttgtt tagatggatc gggattcggc tgtaagcaac
     9241 ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt
     9301 tatttcagtt tctaatcgag cttgcttttc cttttccagc ttcaacaaaa aaactttgtt
     9361 gcgaattgta cggatctccg gaagcttttt actgctcaac cagatattcg cttcaagaac
     9421 ttcctgacgg atcgtccgct gactgacatt gaactcactt gctagatgtt ttgcttttac
     9481 tggatattgg gctgtcatga ttctgtgaag tatttcaatt gttcttgttt tcatagaaat
     9541 gatctccttc tgatttcaag tatatggttc cgcttacata ataaaaagta gatatttctg
     9601 ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa ataagccata tgctttttca
     9661 tctcctcaac gaaaaagaca tacgcctatt attacaaaaa ttttgcccat ctatacaaaa
     9721 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acaactaaac taaaatagtg
     9781 cttcctattt ttcgtttggt tgttcttatt atcgcagatg caagctttta aacaccattt
     9841 agtggagagg ttgtgacaga gatgttcagc cttgagcgat aagaagcgat tttgaaaaat
     9901 cgcttcccat attttacaaa atcgtgcctt attgccgata ggctatctct ggcacactgt
     9961 ttattcagaa aaaggagctg cgacaaggct tttgtcacaa ctccgttcat aaatatttcc
    10021 tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc atcttttttc tttctcagaa
    10081 aaatcaaaat accatatctg agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat
    10141 cgcccatctt ttcgatcgac tgaataatca cagcatctga aatcaattcg gatggcaaaa
    10201 ctattttcgt ggatctgtcg ataatttcct acttcctgaa tagttatttt tttagtatga
    10261 tttcttacac cgtctgcttt gtttttttgc agaactttct gatgttcggc aaacagcttg
    10321 tctgtataac agcttctggc tttttcagga gtttcttctt cccaagcgga ttggatcttc
    10381 atgaatatct tttggatttc cagaaactgt gctttctttt ctttagccgt tcctgacatg
    10441 cgggacaata tctccgcttt cagccttttc ttttgtcttt tattcttgac gatagaaaaa
    10501 gcagaaaccc ctcctactga caaaacagct aatgcaagaa atcccgtttg ctcataacta
    10561 gaaggacctg tacctggacc ataataccca taagaatctc tgccgtaaga acgactgcca
    10621 aaataactgc ccccatatga acgaccacca aatgaacctc tgcccgaact tcgaccgcta
    10681 gatgtacctc caccatgacc acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa
    10741 cataggccca ctaacaaaat gattgctaaa taattgattt ttttcattgt cttcctcgtt
    10801 atcttttatt acaaaaaact atttttatga tagccttgct aaaaggaaga gtcaataacc
    10861 agatgcataa actgtttttt tagcaatttt gtcttattta gtcattgagt aacatcggac
    10921 aaatgtatgt gtcggcagtc cataattcct ctatcttcag catgaacaag cttgcagaca
    10981 agagatctgc gcttcctcca ggagagattt ggttttcctg acaaaagtgc aacagtgctg
    11041 taaatggacg atttggtaaa gcactttgac tagcagcatg agcaagtttt tgcatttcca
    11101 ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc atctagcgaa ctcatcaaaa
    11161 ctaacaacat atgcatttgt ttctgtacat atgaatcatt atctagctcc ttagctgctt
    11221 ggattgcaag cctgatatgc ggatacccta atatcgcttc ttcatatgca ccatttacgc
    11281 catatttttg cttcacccta tgaccatgag tggttttttt atgacgatat tttgtatcag
    11341 gaaaagcagc aagcttgctg acatcatcaa gtattttcga taaagaaaaa ggctgttcca
    11401 tactgatttt ttgtgcaatg acactgatca atagccccat tgcccaaatc gctcctttat
    11461 gggtattgat gccatttgta gcaatcagca ttgccttttc cgcttttcgc ccaatctcag
    11521 caattctttc tcttaactcc tgatcaatag catgatgcca tgctatttga gccatttctt
    11581 caaaaaaagg agttagcgtc ttagcagact taagaaataa agcatagtcc atatcggaat
    11641 gtgcacccgc atccagtgca tccactaatc caggttttgg acttaatgtt acttctgcga
    11701 tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac tgcttttttt cgttcgtcat
    11761 ttatcatgac actcactcct tcttcctact attacttgct gaaacctttt ttgatagcta
    11821 cccaaatgat atgaagtaac cagcttaaag caaatgcaca acttgccgtc attagataag
    11881 ctacaagaat catgactcct gaaggtaact gcagccaact atctttaaac aataataaca
    11941 gtacttggaa aacaaataca ttggctaaat acgtacgata agcataaaca gacaaccatt
    12001 tgaacaatgg caattttggc gaatcgttta caatcatttt ggaagcgcct atgaagacag
    12061 caaagatgat gactaaactg tataaacttt ggatcgtatt caagtaaggt gcattcgcaa
    12121 agctcaagtc tcctggatgt gcgagcaatg aacggacaga aaagaacgcg ataactaagg
    12181 caacaggtaa gaaaagataa cgtactctgt ccaaatattg atagactttt tcgtgataaa
    12241 caaatgcggc taccccataa atcatgaaac tagcaacaaa ccgatctaat aagtaccagc
    12301 tgtcatgata aggcccagtc aatacttgtg tttgatagaa cagataccat gcgacatata
    12361 aaatcgttga accaatcaat actggccata catatttttt attagcaaat actttgtaac
    12421 cgagccaaac aaaaaacggc atgaacaatt ggatctgcag catcataacg gtataccata
    12481 aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa gcttgcagta tccgtatacg
    12541 gagtttgttg ttggactgct ggaaatagcc aaagataagc cgtcgtccat agaagatacg
    12601 gaacgactag ttcaaaaaac ttttctttca aaaattccca ataagacaaa tgctgacttg
    12661 tttttaccat attataaaca attgcaaaaa taaacattgg tgctgtgtat ttaacgatga
    12721 cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg gctgtttgcc aaagcaaagc
    12781 tcaatatcgt ttgcatcata acagccgtta cagctgatac tttctggaag tcacttacgt
    12841 tgattttgat ttttttgttt tcaaacatct ctttctctct ctttttcttt attaccaaag
    12901 ttctttggtt aatttaggtc cttcgactgt tttcaataga atttttttct ctggctctct
    12961 caaatactct tttagtgaaa aaccgccttg ccgagtcact acttgggtat cgaattgctc
    13021 aaaaaaagca ggatgcgatt gaattacctg aattggcaat tcaatcggtg aatttgcgta
    13081 cagcagcaaa tcaaaatcac tggtttcttt tactgccgga atcccagtcg ataattcaaa
    13141 tcctaagctg ccgccgattc cccatttaca tcctgataat atgtttctag cagcatgaaa
    13201 ttgctgataa acaggataat ccatgatttt ctcttgtcta tacgattctt gttcgatgat
    13261 gtcccacgga tgaatgactt gtttgacggc atgttttgag atttctgttg accatctctg
    13321 attgcgggca ggtccacgta aaccaatcgg tagtatttct gcgtgatccg tcgacgcacg
    13381 acgaactgtt ccgaacatct tttcagttag aacgacccat tcaggcaagg tgaacccttc
    13441 tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga atcatttttt atgcatccca
    13501 ttcttcgtcc attttggcac ggactttatt tgtttcgaca cgtccgaatg ttttggctgt
    13561 ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta ttgcggacat cttgaatggc
    13621 ttcattgatt gcttcgatga ctttgctgac attttgttct gaagcagctt ggtcggtgat
    13681 atcttctagg aaatgataga tcgctcctag ttttgtataa ttattgatat cgtaagcgat
    13741 agctggaact ttttcagcag ctgcttcgat ttcagcaacc gaacgttttg tcacacgtgc
    13801 agcactttct ttagacatcg cttggacggt aatttcttta ttgtttaaag caatcaagcg
    13861 gcttgattgt aatccatgtg ctaaaaatgc acctgaaata gcatttcctg caatcaatcc
    13921 aataactgga tgtccagctt gacggagttt tgcataagca gcagcactat tagccaaagc
    13981 cacatggata ccgatcaatt cttcgttata gccgtatgct tggcttggta catcgatcac
    14041 aacgatgatt ggccgtttca cttttttgtc tttatcttct tcgtaaacgt gatccaaaat
    14101 ttgtcccatg cggaaacctt caactaaacc aacttcaccg tttcgtacac ggtacatcgg
    14161 gtttttagga tcaggtacga ttgatgtcac gatggcttct tctatgaatt tacttgattt
    14221 accataatac acagtactaa tagaagaagt tggatttttc atgccagtta gtgccgcaaa
    14281 ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct tctgctactt ctggtaagtc
    14341 aattgatttt gcttgatggt tagcaattgc ttcattgtaa tcgtcaatgg ccattggttt
    14401 tgaaagatct aaagaatcaa gtaatgataa ataaaatgct gagctttcac tgcgatgcgt
    14461 gtcttttcgt tcgtccattg ctgcgatgac agcagctttg attgcttcta cactatcagt
    14521 cactaattca tcaatgattt ttgattctac ccgttgacga gatcctaatg tattccaaat
    14581 caagtctttg tcactagaat caaattctcg aacgcctgct tcttgttcaa taacttctgg
    14641 tccattcaaa ccgactctcg ctttttttgt tgcgattaaa taactcatta atgctgaagt
    14701 aatcgacata ccgccaaatg atcctacacg tcctggaatc aaaccgatga ctggtacata
    14761 ttgttttaga gcaatgatta catttccgat ttctgaaata gataacagtc cgtagtttgc
    14821 ttcttgcaaa cgaacaccac ctgtatctag tacaatgact ggaaagatct catttccttt
    14881 tttattttct tctaacacat gttcaagtgc agcaacgatt tttgcaccgg aaacttcacc
    14941 gataccgcca ccttggaatt ttccttccat agcgattact actgcttgtt tgccattgat
    15001 cgtgccacgg ctgacaacga tcccgtcatc actttcaggg acgattcctt gtgctggtaa
    15061 acttggcgcg atgatttgat cgaatggatc aatcaattca cgtccattgt caagtagagc
    15121 gttcactcgt tctctggcgt ttaattctac aaaactattt ttcatctgaa agttcctcca
    15181 ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc accgaagtca ttgatcacga
    15241 tatctgctga aattggataa cgatcaaaaa atctcgttaa tacattgttc catacttcgc
    15301 cgaagccatc gcttcctgta caaattttta cagtagattc tgttttttct gttggaaaca
    15361 tcaagatttc caaatctcct gaaccgacca ctcctacatg gattttctta ttgatcggct
    15421 gtttcgttgg atatttataa gctaattgtt ccatggatct ctcccctttt tgtaaaacag
    15481 agcatacacg ttctgcttat tcgtctttct tcttccatct gggtaaatgg cgacaaaagg
    15541 tttttctcgc caattttatg accaagttct gaatttcatt ggtggttcat acaggccatc
    15601 tgaccattcg atcaaatcat cgatggtttg tgcagctaat aatgaacgtt tcgcttcact
    15661 acgtttgatg cctagatctt ctggtaattt cacgatgccg gctgcacgca agctatctag
    15721 tgattctttc gtgctagtca ttccaagagg tgtaacaccg ccaattgctg caattgctgc
    15781 ttgtcgttct tcttttgttt ttgctttgta caaataagca attccttctt ctgtcacgat
    15841 atgtgtagta tcttcgctat aaatcatcac tggtgcattg gctaagcctg cttgtttttg
    15901 aacagctagt gcatctaatt cttcgacaaa gactggtttt ttatttgaac catatgtttc
    15961 aaccatttga acaactaatt tttggccttt ccctaatgga tcatcattgt cgcgaagtga
    16021 ttgccatgct ggtgtagaat gacgacgacc gccaggatta tgtcccatgt taggcgcacc
    16081 gccaaaacca gataaacgtc cttttgttac ggtagaagag tttccttcgt aatctaattg
    16141 caaggttgaa ccgataaaca tatcgatggc atattgtcct gcagcttgtg ataatgttcg
    16201 gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca tctgggcgag cagcaatata
    16261 tttttccata ccaacttcgc caccaaaact atggatgctt tcaacccaac ctgattcgat
    16321 ggctggaatc atcgttggat gtgggttcaa tacccagttc ttcgcgattt tcccttttaa
    16381 ccctagtgat tcaccataag ttggcagtaa caattcgata gctgctgtat tgaatccaat
    16441 accgtgattc aaagattgta cgccgtgttc tgcgtagatt cctttgattg ccatcattgc
    16501 cattagaatt tgcaattctg tgatgttttg aggatctcgt gtgaataaag cttctaattg
    16561 ataaggttta tctgctttca cgacaacgtc tacccagtca ccagggatat ctacacgtgg
    16621 taaagaatcg acaacttcgt tgacttgaac gatcacgata ccatctttga aagctgcagc
    16681 ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg tacaagttgc cgtctttatc
    16741 tgctttatct gcagctacta aaacaacatt tggaatcaaa tcgatgaata agcgtccata
    16801 aagttccaaa taagtatgga tatcacctaa tttcattgtg ccgtcttcaa tcatttgggc
    16861 gacacgcaag ctttgtgcac cagcataaga gaaatcgatc ttgcttgcga ttcctttttc
    16921 aaaaatattc aaatgttctg gtcgtgagat actagacatg atcaaatgca gatcattcac
    16981 tttttctgca tctacttttt caagactttc tgaaagaaaa cttgcttgtt tttgattgtc
    17041 gccttctaat acaactttgt cgccgggagc aatcagtttt tccattactt ctacgatttt
    17101 ttcagtttct acatacttgc cattgagcaa gttgctgatt ttttccaatc gctgcgcttt
    17161 tttttcgcgt ttagaagccc agttgcggtt tggtttttcc ataattagtc atctccattt
    17221 ttgatataga aacgtggcgt catcaatacc atgttggtct agattgatcg tacggatccc
    17281 tttatctaca ccaaattttg tatgaatcag ctttgtcaaa gtattgccgg ggggaaattc
    17341 aatcgtcaga tccattccta attcttttgc caagtccatc atttggttcc actgcacagg
    17401 gaaagctagg ttttccaaca aatctttacg aatctcttct gcgtcctgag tggctcttcc
    17461 atccgtattt tttatataca aacatctagc atcagataga tggacagact gcatatacgg
    17521 cttgaatttc ttgaccgtat cattcatcaa gagacagtga gatggcacag ggactttcag
    17581 cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc aacgtaacgg ataaaccttc
    17641 gatactccca gataacgtat gttgcatcgg acaattttca tttgacagat aaactggatg
    17701 gtcaggatca aatgtttttg ctacaatttc ctcagcttcg cttctcgtca cacctactac
    17761 tacgcccatt gcataaccgc ttggataagc ttctttcatt aagactgcgc ggtgatagac
    17821 tagttccgct gcattctcaa atctcaaaga tcgacaagca acagctgcac aaaaagcacc
    17881 aatcgaatgt cctgctgcaa tatcaggctg aataccgttt ttttccattt catctaaata
    17941 aaatactgct tttgtcagta atgctagctg gataaaaacc gtatcttgat agttttcttc
    18001 tttatcaact aaccgatacc ccgtcacatt ttctaaacgt tccatgtaca tttcagggat
    18061 atccgctaac atccctaact tctgactgcc ttgtccagga aataagaaac ctgtactcat
    18121 gagcctcgcc tcctcagcac ttactttttc cactgctatc ttagtcaatt tctgctataa
    18181 taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt atggatggga tggataatta
    18241 tgaaattgct tcatctgcag tattttaaaa aagtattcga aacaaaaaat gttacccaag
    18301 cagccaaaga attatttatt tctcaacctg ccttgagccg tgcgatcaaa catttggaaa
    18361 acgaattagg tgtccctttg ttttaccata gcggcagaaa cattgagacg accgtttatg
    18421 cagaagaatt ttatccatat gcagtcaaga cattagacac attagaagaa ggaatcaaat
    18481 tattgtcttc ggtcaatgag aagatcgtta cttccgtcat attgtattta gaggtagcct
    18541 ctgtttctat tccgaacttg gtccgaattt ttatggaaaa gcatcctgat atccaattaa
    18601 cgatcatgca acatcattta ccagatgata ttaccgatcc tgtactgtac atcacttcgg
    18661 agatgaagcc tggcatgacc aatattccta tcatgaaaga acctgtttat gtcgccattc
    18721 ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt agaagatatc caacgcaccc
    18781 ccatcttgat gctgtcaaag agcaacgcct tccgtcatac actggatgca gcacttgaag
    18841 cgaaaagtat cgaattgacg atcagttcga cgacagatga tcctgcgaca ttacgttcta
    18901 ttgtcaagca aggattagct gttagctttt tcccaaaagt ttcttggtct tacgataaaa
    18961 atgatccttt cgtcttgcgg gaaatcaaag atttgccctt gacacgaaca atttatttat
    19021 cttctagctt cacagaagat catcctttga tcaaaatgat tgcaaaaacg atgcgggaat
    19081 tttatttagg aaaataaaaa aaaaagagct gggacaaaat tcttgtcgca gctctttttt
    19141 cgaacaaacg gtgttccaga agtaatttct tagaaaataa gccgaaattt cccaaaaatt
    19201 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctaaacattt ctgtcacaac
    19261 ctcttctcta ttgtcctaga cattcattgc gttgatttaa aacttccatc aatcaaatct
    19321 gctagttcat ctagcttttc tattttacgt agatcaatag agatccaatg ttctttattc
    19381 atgtgatagg ctggaaaata cccctctttt cctcgcaaaa ttccgccaag ttcaggatcg
    19441 accttcaaat caagaatatt gatgatccta tggtcacttg catcatcata gattttttct
    19501 tctggaacag acatcaggac cccaaaccat tttttgcttt tttgatgtct gaatacgcaa
    19561 taatcaggaa attttttaaa aatatattct ggagacaaat tgtatgtttc attgatgtga
    19621 tcgattagtt cttttttcgt tagcatctgc ttccatctct ttcttattga ttcgctttat
    19681 tcaaagatac cacgtaagaa tagaaaaatg aatacaagta gttgctaatc aaatacttcg
    19741 atatttttct tttttatcaa ctcttaaatg ttcaatccgc ctgtgttggt cttgctacca
    19801 tctactatta aaatgtttat tattgtttgc ccacctattt tttgatttta gtggtatcat
    19861 ttttcaagtt catgatgaaa ttcttatcat cagtaaatgc tccagaaaaa taaacaatga
    19921 ttgaaaaatc agttgaaact tgaccaccgt ttattttctc cgtattctta gaatagaagg
    19981 gagtctgatt gatgtattca ctaatgaaaa aaatcattac cgaaaaagat attcgacgtc
    20041 atgtttcttt agtagaacaa ttgctgaatc atacaaagat aaccgtaaat gaattagctg
    20101 aaatcatagg gacaaccgaa cgaacgattt tttctgacct tcaatctatt cgctctcagc
    20161 tgcccgaagg ctgggacatc atcagcgatc aagcaggaat cagtttgcaa aaccagcaaa
    20221 atttattaac aaatgacctt tgggagattt ttttcaaaca atcagtcagt gtcgaactgc
    20281 tgaaaaatct gctttttaca aaaaaagttg ctgtccctga ttttctagcc gaccatgggc
    20341 tttcttatgg gacattgaaa agacatgtga ctaaaatcaa tcagcgttta gcaagttacg
    20401 acttgcagat cgatttgacg aaatatacag cttgtattct tggaaaagag cgtgcgattc
    20461 gtacttttta tcatcgattg ctgattcctt tcactcataa caactatttt ttcgaggatt
    20521 attcgatcca tgaatcccat tattttcaat ttttaagaaa cctcagtcaa actgaactag
    20581 cggttgaaac agaagagatt ttcggaacat gttggttctt tatcaatacg atccgtataa
    20641 aagcgaattg ccgattagat tcttctatcc ataccaacag cacactctcc tccctgtatg
    20701 actctgcatt gaaaaaattg tatttaacgg aaggtattta tcttaaagac actgagcttt
    20761 cttttgcttc tttctgcttt ctggaaagtt ggaattacaa caataactat ggacaagaaa
    20821 ccgctcgctg cctgcatcat tctccatttt tagaggtcct cgaaacgttt gtagaggaac
    20881 tagcttcaga gctgtctctt gatcaactaa aaaaaacatc tctgactgat aacttggctc
    20941 tcctcatatt aaaatatcat gaatcgccga tattgattga agcattggac cgtcaatacc
    21001 atcgtttttt agaaacatat gatcagcaat atgcacactt gtatcaacag aaagagaatt
    21061 tactgaacga tctaaaaaaa gaaatagaaa tcaatgaacc aaattacttt ctccaactct
    21121 tgtctctact gatccaacaa acgatttttt ctatcaaacc gaatttgttc aatgtctatt
    21181 ttattttcca aggagaacct tcatggaagg cgttcttgca gcaggaatta aaagattact
    21241 taggaaaacg tgtctgcttc ttacctatcg aactaaccga tctttccact atttcttttg
    21301 aaaaaacaga tatcttgatt tccaatttcc ctctagattc tctagatatc cctattgtat
    21361 acatttcttc tattccaacg aaaaatgagc tcaatcgttt gactgaactc actttcaaaa
    21421 cttttttata gtaacggcta gcgactgtat tctctagctg ttttttcttt tttgcgaaac
    21481 agtttgttca tgatcccttt ttctatttct acaactaaga atagaagaat cacatttgct
    21541 ccaatcaata actgttgtgt catcgttaaa cttactgtac cgatcagctg ctggccgact
    21601 ggtagaaaaa taaccatccc ttgcaataaa gcaaggatac ctaatgaaag aaaaagcgct
    21661 ttgttttgca aaagaccttg gttcaatgac ggcttggaca actctctgca attgatcatg
    21721 taaacagctt gagctaagac gatattttgc agaagaatgg tttgctgtaa gctttgccct
    21781 tcaaactgca tagccaataa ataggcagga accatgatca gtagggagac atagatgatc
    21841 cgaaacaaat gataaccaga taggattcct tgctttggat ctcttggtgg gcgcttcatg
    21901 atttctggat ctggcgactc gaaccctagt gcataagata aagtgattgt cgttaccata
    21961 ttgacccata aaatctgtac tggggacaaa ggtagcgggc gattcagcat aagtgcccaa
    22021 atcaagatca atccttgagc taatgcagtt ggcaagaaga aattgatggt tttttgcaaa
    22081 ttatccaaaa tccgccggcc ctcttttacg gcttttgcaa tcgtatggaa attatcatct
    22141 cctaaaacca tatcagctgc ctgtttgctt acttcactac ctttgattcc catagcaatt
    22201 cctacatcgg atcttttcaa agcaggtgca tcatttacgc cgtctccggt cattccgacg
    22261 atttctccat ttttctgcaa agctttaaca attcgcagtt tatgttctgg tgtcgttcga
    22321 gcaaagacat cgactattgg cacttgctga atcaactctt catcagacat aagatccaaa
    22381 tcgatccctt ctaaaacttt ggcagtatgt tttaagccaa tttgttccgc aatcgctttt
    22441 gctgtgtctt tgtgatcccc tgtaatcatt ttcactgaga tccctgcttg ttggcattcc
    22501 tttacagctt tgactgcact ttcttttggc ggatcgatca atccggcgat ccctgcaaaa
    22561 gttaattggg ttaaagtctc atgcgtgagt tcctgtttcg aatctacttt tttataagca
    22621 aatcctaaaa cacgctgacc ttttctcgca aattctgcag ctgtctgact ccatgcttct
    22681 tgttctgcag aagaaagagt tgatttagca aataatactt ctggtgcacc tttgacgtaa
    22741 atcaccgctc ctttttcctc cgcatgcatc gttgccatat atttgtattc agaactgaac
    22801 ggaatctttg cttctatttt tttcaaaggt agattggctg tttctgtgtg tcgaatcagc
    22861 gcaacttctg tcggattgcc acttaaattt tctgttttct gttcttcttt tgtttttaat
    22921 tcctgacagt ttttcataat ggacaacacg caggcttctt cttttgcagc tacatccaca
    22981 actgtcattt cattttttgt caatgttcct gtcttatctg aacagatcac tgtcatagaa
    23041 cctaatgttt ctaccgaagg aagtcctttg atgatcgcct tttcttttgc catttcatgt
    23101 actccaacgg atagaatcat ggtcaaaaca gctggcaatc cttcgggtac cattgacacc
    23161 accaaagcaa tcgtagccga aagaaggata tgccaatcca taccatatcg aaatgtcgta
    23221 aaaataacta agaataggct caaacaaaga atgccttgaa aaatttgttt gtttaatcgg
    23281 ttgatttttt taatcagagg cgtcgtttgt tgttcgaccg attgcaatgc ttgattgatt
    23341 ttaccgattt ctgtcgtgtt tcctgtttct acaacgacac cgactgcaga accagattgt
    23401 acaagcgttc ctgaaaaagc catatttttc tggtcgccga tcgattcttc ttctgtcaaa
    23461 acggcatgac ttttttcaga tgcttctgat tctcccgtca aaatcgattc ttccactaat
    23521 aaatcatgtg cttcgattaa tcggatatct gctggaacga catctccggc acgtaagaca
    23581 atgacatctc cttttaccag ttctttcgca gggatttttt cttgcatccc gtttgatagt
    23641 actatcgctt cttgtcccat cagctgcttc aaaccattca atgattcttc cgcttttctt
    23701 tcttgccagt aactaacgag tccattgacg atcactacga ataaaataat acttccttca
    23761 atatattcac tagaagcaaa tttcaaaaaa gaggcaatga tcaacacgat catcagcaga
    23821 tcagtaaaat gctttgctat ttttttccat cgttttgtct gctgccttgc ttttatttca
    23881 ttatatcctt gatcagtcag gcgtttctgt cgttcttctt gcgacaagcc ttctgttgat
    23941 gtttgatagt ttttcaatac atcttctacc gtcaatctgt accagttcat cctatcccct
    24001 cctgtttaat tgatactgtt attatacgca gggaagtttt ttcaggcgta tcgattattt
    24061 caggggggtc tgaaataatt cgttttgcaa aatgtatctg ggcctaactg atatttctat
    24121 tgcttattct tttatataca aaatttaaga atacaaacca gataaccaat aaaaaaggaa
    24181 taaagcgtgt cgtgctttcg aataacaagc tgaaagcaat tgaagtaaaa aacaccaaca
    24241 aagcaagatc tttatatctt tgtgtagtgt tccgtttctt ttctttaata aattgaacat
    24301 gtgcacacaa gatcgaagac cacacaaata gaaaacaact cgttgtcatg cctgccaaaa
    24361 gctgaaaaaa caatcgacta tcagctacaa aaaaatgtag acaaagaccg gcaaaaaata
    24421 acattcctgt aattaaaata ccgtgtaaag gtacgccttt cttagaaagc gcacaaaacc
    24481 aatggggcaa acttttttct tttgctgttt gatataaaat cctgctagta ctgtagactg
    24541 cactatttgc tgaggacaaa gaagcaacca aaatcattag attgacaatt gctgctccgc
    24601 cttggcaatg gatcacttgc agcatagtca ctaaaggact ttcttttaaa tctaaacaat
    24661 gccatggaac aacaaataaa atcgacaaaa tcgttcctat atagaaaaag agaatacgcc
    24721 aaggcagaca gttgattgct tttttcaaat tcttttgcgg atcacttgtc tcgccaacgg
    24781 ttaagccgat catttcaatt ccagcaaaac tgaataaaac cataggaaat gagactacca
    24841 ttccagcaaa gccaaacggt agtaattcat cgatctgtcc aaaattttct ttgattccga
    24901 tagactgggt atgaaaatct gagacactca atcctatacc caataagata aaaaggaaaa
    24961 tccccaagat cttgatgatt gagaaaaaag attccaattc accaaaccac ttgactgcaa
    25021 caaggttgac tgccattaag atcattagtg cgctacctcc gcttatcaaa gctggtacat
    25081 gtacaaacca gaaatggata tacaatccta ttgctgttaa ttccgcaagt ccagctccaa
    25141 gccagcaaag ccaataagtc caattgacaa aaaatgccca ctttttgcct aaataatagt
    25201 caacgaaatc actgatagag ttaaatgtac tatttgctaa aaataaatca cccatggccc
    25261 gcatcaaacc ataaatagcc aaaccgcata tcaaataaac gagaataaca gctggccctg
    25321 ctgatgcaat tgcctttgct gaacctaaaa ataatcctgt tccaattgcg ccacctaatg
    25381 cgattaactg gatatttcga ttagttagag tccgtttcat tgccggtttt tccatcaagt
    25441 tcaccttttt ctttttcaag cagcatggcg acttcctgtt cccaagaagg tttctttatt
    25501 ttttcaatac agagtggtac tacaaacaaa atgacactga tggctgtgac tgcaattaga
    25561 ttgatccatg ttccagcttt catcgtcagt ccgacagaaa atccgattgc cgcaaataaa
    25621 acaataacga cagtccaaac tccaagattg ctctttttct ctttttttcc aattctaaat
    25681 ggtcgattca actttgcatg tgtataacgc aatctcaaaa ctgcgatccc catgatgaca
    25741 tagacgatac agtagataac agtagttgaa tttacgagca tcaaaaatgc ttgattgacg
    25801 ccaggaatca gcaaataaaa aagtgcaaag attgagataa tgacagactg agtcaaaata
    25861 acggatttgg atacgtcaaa ttgattcgtc ttccaaaaat tgaattttgg tggatacgca
    25921 ccttttctag cactttctgt cactgtcttt gctggtcctg aggcccaagc agataattgg
    25981 actgctacac caatgaagac aagtaaacta aacagattca caataagatg tggcaaacca
    26041 agaatttggc aataaatcga aatagattgc gcgatattat tcaattccat ttgtcctttt
    26101 ggtacgacat ttgctacaac taaagcattc aaaatattga cgaaaaatat aaaaattaac
    26161 gcggcaaata ctccgcgaat atacgtttta actggatttt ccaatcgagt gatatacaca
    26221 gaggacattt cgataccagt aaaaataaac atgatcggcg caaaataaac aaatgaggat
    26281 gcagtcgtag tatctggtat taatttgtcc catgaaaaac tgcctaatgt tccggttggt
    26341 acgatcccaa ctttgatcca cgcagcaaac cctaataaga gcatcatcgt caaaggcacg
    26401 tacaatccta accaaacacc gacttttccg ccgatttttg ccatatcaaa tttcaaattc
    26461 aaaagagtga tgatccaata aacgactaaa atgacaacta atgtaaattt gctgttcaaa
    26521 ccaagcggca cattatcgat aatattacca aacaacggag cgagtgctga agcgaccatc
    26581 accatcccag gaaacatttg gacccataac agccatgaga caacgaaccc ccattttttt
    26641 cctagagcat tcgataccca aagttctgga ccaccttttt ggggaaacat tccagcaaat
    26701 tcaccagaga tcaatgagat tggaaaagcg tacaataata ctgcaaccag catgtaaaaa
    26761 atcatcgtcc agctcgttgc tgctacatct ggaatatttc gtacacttgc tacaagggcg
    26821 caggtcattc cgataaaggt catggtcgtg atttgctttg atgaaccagt atctttcatc
    26881 tttattcctt ctttcaatag cgacatatgt ttttgttttc ttttattttt gttcaagaac
    26941 agaaatgaca aggtttcatc gattcagtac aaaacccagt atcaccgaaa catccattga
    27001 acgatcttag tgaactgggt ttgatacatg aaacgaacga tccaacttat agaacgtttt
    27061 ataaaaaaga atatcttggc gaggaatata tgaaaaacag ggggaaatac aattgttttg
    27121 atgaagcaaa taattgtctg ttctccatat gtctcttgtt attctttcac acaaaaatca
    27181 gttctattgg atgatttcgt ttagtttctt ttccattgct ttggcgattt tttttgtata
    27241 aaaattgaaa attcgatcat cattcaaata aggagtcatg atagccgcac gtagcagagt
    27301 gattttctgt tcttttttcc attcttcttt cgttagaccc attctttcaa caaaacgaat
    27361 cggagaatca ccgtaatctt cttgtgtaaa tgtagtatga gaagtaataa accgttcacc
    27421 ataaacatcg ccatccatat aagaagaccg atcgaacatt ttttcattta attcattgat
    27481 agcgttcaaa tctgtacagc cttgttcttt gaatacccaa ttgaccatat taaaatcagg
    27541 atgattcaat ggatagactt cgattgtttt tcctttaaca gtaaaagtca gatgatcgag
    27601 aaattctctg aatctttgag ctgcctcgat tgaagcccca atcaattgac catatccggt
    27661 cacattcaat ggcaatacac gatgtgctgt ccagactgcg gcggcggttg ctccagcttt
    27721 tgatccttcc aaaatataag ctcctaacat atctggagcc tttactgatt tttcaaacac
    27781 atatggcgcg aaataagaaa tgatatttct catattttta tgtttgatta cgattcctcc
    27841 agcagcataa gggacatagc ccatcttatg aggatctatc gtgacagaat ccgcttctga
    27901 aatagctctg aacccttcat atacctcttt gtcaatcgtt acgcagtggt ggaaaacatg
    27961 atgttcttca aagaattcag ctaaggaagc atacggcaca aattctcctg cttcgtttag
    28021 aaacagtgaa cgcgcatagc cgccatatgc ggcatcaaca tgtaaataga aatagatccc
    28081 ttcatctttt aacctttctc ttaattggac gattttatct acactgtcta cttgaccttc
    28141 ttctgtcgtt cctacgacag caaccacacc tagaattggg attttttgtc cagctaattc
    28201 acgaattgtt ttttccaaag cattaatatc cattcgataa tcctcttgaa ctgggatagc
    28261 aaccatctga tctaagccga ctccgcaaat atctaacgcc ttcatccaag aatagtgttt
    28321 tgtttgaggg accagccatt tccctaatct ttggatattt tttccgctac gggaagaagc
    28381 tgctttgacc tcatccattt cttcgtccgt aaagctttcg gtcatttcca aaatttcttc
    28441 aacagataaa ttcaaaagtt cccactcaga catttttttc actttttcag gcagcacttc
    28501 cttgacagct aaaggaatcg atttgatgca acgggcgtac cataatcctt caagattagc
    28561 aatagaacca tctgctgcaa tgtgtcccca tccgtctgtc atattgaata atgaagcaaa
    28621 atcctgtcct acttctgctt ccatttgcga agttgccata gaagattcca atgcaacatt
    28681 atttgggttc cacagcatcg catagttgta agctaataaa gcaggcatca atgtttcagc
    28741 attcatttgt ccccagtaac ggcctgctga atgccatggg atcgatcctg cgcgtaatcg
    28801 ctgactgact tcatccaaga cctctatcat atggtcgcgg gtggctagat aatctggagt
    28861 gagcttatct ccttcgctaa ttgcaggcat atctgaagga atataatttt ctctccatcc
    28921 aagatgttca tctaccatct tgttcaacaa cattttataa actggtccat tttcagcttt
    28981 gtcgcctata aagacggcct tgatatccat atctttcatt tttcttccct ccatattttg
    29041 ttggtcaatg tcttgcagat acatgtgttt atctgtaaca cctcttcact atagcgccaa
    29101 aaatgagaaa ttttttttat aacatcaaat ccacgtagat ttttacaaac aaaaataaat
    29161 aaagtcggat ttttctgata attcaacgca aaaaagcgaa caatttttca ataaatcagt
    29221 cttctaaaat agataaccta aaaaaagcga acaaacaaa
//
LOCUS       DAHUWM010000009        27894 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHUWM010000009 DAHUWM010000000
VERSION     DAHUWM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.009"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="NK788_000257"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="NK788_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCH6768636.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="NK788_000258"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="NK788_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCH6768637.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="NK788_000259"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="NK788_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCH6768638.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="NK788_000260"
     CDS             1642..2274
                     /locus_tag="NK788_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCH6768639.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="NK788_000261"
     CDS             complement(2276..3181)
                     /locus_tag="NK788_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCH6768640.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="NK788_000262"
     CDS             complement(3266..3925)
                     /locus_tag="NK788_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCH6768641.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="NK788_000263"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="NK788_000263"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCH6768642.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="NK788_000264"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="NK788_000264"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCH6768643.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="NK788_000265"
     CDS             6168..7253
                     /locus_tag="NK788_000265"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCH6768644.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="NK788_000266"
     CDS             7250..8314
                     /locus_tag="NK788_000266"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCH6768645.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="NK788_000267"
     CDS             complement(8563..9366)
                     /locus_tag="NK788_000267"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCH6768646.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="NK788_000268"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="NK788_000268"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCH6768647.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="NK788_000269"
     CDS             complement(10244..11251)
                     /locus_tag="NK788_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCH6768648.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="NK788_000270"
     CDS             complement(11258..11902)
                     /locus_tag="NK788_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCH6768649.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="NK788_000271"
     CDS             complement(12002..12760)
                     /locus_tag="NK788_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCH6768650.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="NK788_000272"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="NK788_000272"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCH6768651.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="NK788_000273"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="NK788_000273"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCH6768652.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="NK788_000274"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="NK788_000274"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCH6768653.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="NK788_000275"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="NK788_000275"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCH6768654.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="NK788_000276"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="NK788_000276"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCH6768655.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="NK788_000277"
     CDS             18158..19027
                     /locus_tag="NK788_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCH6768656.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="NK788_000278"
     CDS             complement(19153..20052)
                     /locus_tag="NK788_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768657.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="NK788_000279"
     CDS             complement(20217..21041)
                     /locus_tag="NK788_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCH6768658.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="NK788_000280"
     CDS             complement(21052..22347)
                     /locus_tag="NK788_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCH6768659.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="NK788_000281"
     CDS             complement(22363..22842)
                     /locus_tag="NK788_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCH6768660.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="NK788_000282"
     CDS             complement(22848..23876)
                     /locus_tag="NK788_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCH6768661.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="NK788_000283"
     CDS             complement(23893..24999)
                     /locus_tag="NK788_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCH6768662.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="NK788_000284"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="NK788_000284"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCH6768663.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7866 a         6126 c         5036 g         8866 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttt
//
LOCUS       DAHUWM010000010        27427 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHUWM010000010 DAHUWM010000000
VERSION     DAHUWM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.010"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            167..475
                     /gene="rpsJ"
                     /locus_tag="NK788_000285"
     CDS             167..475
                     /gene="rpsJ"
                     /locus_tag="NK788_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCH6768664.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            506..1135
                     /gene="rplC"
                     /locus_tag="NK788_000286"
     CDS             506..1135
                     /gene="rplC"
                     /locus_tag="NK788_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCH6768665.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1163..1786
                     /gene="rplD"
                     /locus_tag="NK788_000287"
     CDS             1163..1786
                     /gene="rplD"
                     /locus_tag="NK788_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCH6768666.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1786..2076
                     /gene="rplW"
                     /locus_tag="NK788_000288"
     CDS             1786..2076
                     /gene="rplW"
                     /locus_tag="NK788_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCH6768667.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2115..2948
                     /gene="rplB"
                     /locus_tag="NK788_000289"
     CDS             2115..2948
                     /gene="rplB"
                     /locus_tag="NK788_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCH6768668.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            2991..3269
                     /gene="rpsS"
                     /locus_tag="NK788_000290"
     CDS             2991..3269
                     /gene="rpsS"
                     /locus_tag="NK788_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCH6768669.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3292..3639
                     /gene="rplV"
                     /locus_tag="NK788_000291"
     CDS             3292..3639
                     /gene="rplV"
                     /locus_tag="NK788_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCH6768670.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3653..4309
                     /gene="rpsC"
                     /locus_tag="NK788_000292"
     CDS             3653..4309
                     /gene="rpsC"
                     /locus_tag="NK788_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCH6768671.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4312..4746
                     /gene="rplP"
                     /locus_tag="NK788_000293"
     CDS             4312..4746
                     /gene="rplP"
                     /locus_tag="NK788_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCH6768672.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4736..4924
                     /gene="rpmC"
                     /locus_tag="NK788_000294"
     CDS             4736..4924
                     /gene="rpmC"
                     /locus_tag="NK788_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCH6768673.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            4949..5215
                     /gene="rpsQ"
                     /locus_tag="NK788_000295"
     CDS             4949..5215
                     /gene="rpsQ"
                     /locus_tag="NK788_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCH6768674.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5273..5641
                     /gene="rplN"
                     /locus_tag="NK788_000296"
     CDS             5273..5641
                     /gene="rplN"
                     /locus_tag="NK788_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCH6768675.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5678..5986
                     /gene="rplX"
                     /locus_tag="NK788_000297"
     CDS             5678..5986
                     /gene="rplX"
                     /locus_tag="NK788_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCH6768676.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6014..6553
                     /gene="rplE"
                     /locus_tag="NK788_000298"
     CDS             6014..6553
                     /gene="rplE"
                     /locus_tag="NK788_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCH6768677.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6572..6757
                     /locus_tag="NK788_000299"
     CDS             6572..6757
                     /locus_tag="NK788_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCH6768678.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6793..7191
                     /gene="rpsH"
                     /locus_tag="NK788_000300"
     CDS             6793..7191
                     /gene="rpsH"
                     /locus_tag="NK788_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCH6768679.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7223..7759
                     /gene="rplF"
                     /locus_tag="NK788_000301"
     CDS             7223..7759
                     /gene="rplF"
                     /locus_tag="NK788_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCH6768680.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7920..8276
                     /gene="rplR"
                     /locus_tag="NK788_000302"
     CDS             7920..8276
                     /gene="rplR"
                     /locus_tag="NK788_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCH6768681.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8297..8797
                     /gene="rpsE"
                     /locus_tag="NK788_000303"
     CDS             8297..8797
                     /gene="rpsE"
                     /locus_tag="NK788_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCH6768682.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8812..8991
                     /gene="rpmD"
                     /locus_tag="NK788_000304"
     CDS             8812..8991
                     /gene="rpmD"
                     /locus_tag="NK788_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCH6768683.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9035..9475
                     /gene="rplO"
                     /locus_tag="NK788_000305"
     CDS             9035..9475
                     /gene="rplO"
                     /locus_tag="NK788_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCH6768684.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9475..10770
                     /gene="secY"
                     /locus_tag="NK788_000306"
     CDS             9475..10770
                     /gene="secY"
                     /locus_tag="NK788_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCH6768685.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10832..11479
                     /locus_tag="NK788_000307"
     CDS             10832..11479
                     /locus_tag="NK788_000307"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCH6768686.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11670..11888
                     /gene="infA"
                     /locus_tag="NK788_000308"
     CDS             11670..11888
                     /gene="infA"
                     /locus_tag="NK788_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCH6768687.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11921..12037
                     /gene="rpmJ"
                     /locus_tag="NK788_000309"
     CDS             11921..12037
                     /gene="rpmJ"
                     /locus_tag="NK788_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCH6768688.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12055..12420
                     /gene="rpsM"
                     /locus_tag="NK788_000310"
     CDS             12055..12420
                     /gene="rpsM"
                     /locus_tag="NK788_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCH6768689.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12448..12837
                     /gene="rpsK"
                     /locus_tag="NK788_000311"
     CDS             12448..12837
                     /gene="rpsK"
                     /locus_tag="NK788_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCH6768690.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12917..13855
                     /locus_tag="NK788_000312"
     CDS             12917..13855
                     /locus_tag="NK788_000312"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCH6768691.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13883..14263
                     /gene="rplQ"
                     /locus_tag="NK788_000313"
     CDS             13883..14263
                     /gene="rplQ"
                     /locus_tag="NK788_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCH6768692.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14535..15374
                     /locus_tag="NK788_000314"
     CDS             14535..15374
                     /locus_tag="NK788_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6768693.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15350..16219
                     /locus_tag="NK788_000315"
     CDS             15350..16219
                     /locus_tag="NK788_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6768694.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16209..17006
                     /locus_tag="NK788_000316"
     CDS             16209..17006
                     /locus_tag="NK788_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCH6768695.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17209..17955
                     /gene="truA"
                     /locus_tag="NK788_000317"
     CDS             17209..17955
                     /gene="truA"
                     /locus_tag="NK788_000317"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCH6768696.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18288..20957)
                     /locus_tag="NK788_000318"
     CDS             complement(18288..20957)
                     /locus_tag="NK788_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCH6768697.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21195..21383)
                     /locus_tag="NK788_000319"
     CDS             complement(21195..21383)
                     /locus_tag="NK788_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCH6768698.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21653..22015
                     /locus_tag="NK788_000320"
     CDS             21653..22015
                     /locus_tag="NK788_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6768699.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22067..23749
                     /locus_tag="NK788_000321"
     CDS             22067..23749
                     /locus_tag="NK788_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCH6768700.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            23866..25902
                     /gene="recG"
                     /locus_tag="NK788_000322"
     CDS             23866..25902
                     /gene="recG"
                     /locus_tag="NK788_000322"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCH6768701.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            25951..26952
                     /gene="plsX"
                     /locus_tag="NK788_000323"
     CDS             25951..26952
                     /gene="plsX"
                     /locus_tag="NK788_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCH6768702.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27074..27316
                     /gene="acpP"
                     /locus_tag="NK788_000324"
     CDS             27074..27316
                     /gene="acpP"
                     /locus_tag="NK788_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCH6768703.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         9119 a         4962 c         5984 g         7362 t
ORIGIN      
        1 caaaggtgcg tttactttag tgaattgcac agacgtaatc agaatgattt cgtcggctaa
       61 gaaacgagag gttgcgacac gcccggacgc tttgccacga acgagcgtga cggaaatttt
      121 cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa
      181 aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag cggataaaat
      241 cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt ccaattccat taccaactga
      301 acgcagtctt tacacaatca tccgtgcgac tcataaatac aaagattcac gcgaacaatt
      361 cgaaatgcgt acacacaaac gtcttatcga cattgtgaac ccaacaccaa aaactgttga
      421 tgctttgatg aagctagact taccatctgg tgtaaacatc gaaatcaaat tataaaacta
      481 aaacaaaaat aatggaggtg tactcatgac caaaggaatc ttagggaaaa aagtgggaat
      541 gacacaaatc ttcactgaat ctggtgaatt aattccagta actgttgtag aagctactcc
      601 aaacgtagta ctacaagtga aaacaatgga aacggatggc tacgaagcta ttcaagttgg
      661 ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc
      721 aaacacggct cctaagcgct tcattcgtga attcaaaaat gttgagctag gagaatatga
      781 agtaggaaaa gaaattacag tagatgtttt ccaagcagga gacattattg atgtaacagg
      841 tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa gccgcggacc
      901 aatggctcac ggttctcgtt accatcgtcg tcctgggtca atgggtccag ttgcacctaa
      961 ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca
     1021 aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt
     1081 tcctggagct aaaaaatctt taattactat caaatcagct gtgaaagcta aataattgta
     1141 ggaagaaagg aggaactaag gaatgccgaa tgtagcatta ttcaaacaag atggaagcca
     1201 aaacggtgaa atcacactta acgaagaaat cttcggaatt gaacctaatg aaagtgttgt
     1261 ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa
     1321 ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc
     1381 acgtcaagga tctatccgct caccacaatg gcgtggaggt ggcgtagtct ttggaccaac
     1441 accacgttct tacagctaca aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt
     1501 attgtcagaa aaagttgctg aaaacaactt ggtagcaatc gaaggcttga acttcgacgc
     1561 accaaaaaca aaagaattca aacaagttct tgctaactta tctatcgact ctaaagtatt
     1621 ggttgttcta gaaaaaggta acgactttgc tgctttatca gcacgtaact taccaaacgt
     1681 ttctgtagta acttctgata atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt
     1741 agcaacacaa actgctctta ctcaaattga ggaggtgctt gcataatgaa cttactagac
     1801 gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg ccatggatga caagaaatac
     1861 acttttgaag tggacactcg cgcaaacaaa actttagtaa aacaagcggt agaagctgct
     1921 tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg
     1981 ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat
     2041 tcaaaagaaa tccaattatt cgaagctgct gaataagcac taatagataa ctcattaaat
     2101 taggagggaa aaacgtggcg attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca
     2161 tgacaggttc tgattttgct gaaatcacaa catcaacacc tgaaaaatca ttgttacaac
     2221 cattgaaaaa ccatgctgga cgtaacaaca acggccgcat cactgtacgt catcaaggtg
     2281 gcggacacaa acgccaatat cgcgtgatcg acttcaaacg taacaaagat aacgttgttg
     2341 caactgttca aacgatcgaa tatgatccaa accgttcagc taacatcgcg ttagtacact
     2401 atgaagatgg agtgaaagct tacatcttag cacctaaagg actagaagtg ggtatgaaac
     2461 ttgtttcagg tccagaagca gatatcaaag tcggaaatgc tcttccatta gaaaacattc
     2521 cagttggtac tgttatccac aacattgaaa tgaaacctgg taaaggtgga caattgatcc
     2581 gttctgctgg tacaagtgct caagtacttg gtaaagaagg caaatacgta ttgatccgct
     2641 tgaactctgg tgaagttcgt atgatcttag ctgcatgccg tgctacaatc ggttctgtag
     2701 gtaacgaaca acacgaatta atcaacatcg gtaaagctgg acgctctcgt tggatgcgta
     2761 aacgcccaac tgtacgtggt agcgtaatga accctaatga tcacccacac ggtggtggtg
     2821 aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg
     2881 gttacaaaac tcgtaacaaa aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa
     2941 ataaataatt actttgacca tgtgtcgcac attttgagag gaggttcacc atgggtcgta
     3001 gtttaaagaa aggacctttt gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag
     3061 gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt
     3121 ttgttggata taccatcgca gtttatgatg gacgtaaaca tgtaccagtt tacatccaag
     3181 aagacatggt aggacacaaa ttaggtgaat ttgcacctac aagaacttat cgtggacatg
     3241 ctgctgacga taagaaaacg aaacgctaat ttgaggggag gataaatcca aatggcagaa
     3301 caaatcacat cagctaaagc aactgcaaaa acagttcgca cttcacctcg aaaagcccgt
     3361 ttagtaattg accttatcag aggtaaaagt gtcgcagatg caatttcaat cttgaaattc
     3421 acaccaaaca aatctgctgg aatcattgaa aaagtattga tgtcagcagt tgcgaatgca
     3481 gaaaataact ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa
     3541 ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa
     3601 cgtacaagtc atatcacggt agtcgtatca gaaaaataag gagggataat cagtgggtca
     3661 aaaagtacat ccaattggaa tgcgtgtagg catcatccgc gactgggatg ctaaatggta
     3721 tgctgaaaaa gagtatgctg agttcttaca cgaagattta agaatccgta aatttatttc
     3781 aactaaactt gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt
     3841 aaacatttca attcacacag ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt
     3901 cgaaagccta agaaaagaat taaacaaatt aactggtaaa agaatccata tcaatatcgt
     3961 ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt
     4021 agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc
     4081 tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag atatcgctcg
     4141 ttcagaagga tactcagaag gaactgttcc tcttcatact ttacgtgcgg acattgatta
     4201 cgcatgggaa gaagcagata caacatacgg aaaactagga gttaaagtgt ggatctatcg
     4261 tggagaaatt cttccaacta aaaaaaacac tgagaaagga gggaaataat catgttagta
     4321 cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc
     4381 ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg ttgattcaca ttggatcaca
     4441 aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa
     4501 gtatggatta aaattttccc tcacaaatct tatactgcca aagcaattgg ggtacgtatg
     4561 ggttctggta aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg
     4621 tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag cgttacgtct agcttctcac
     4681 aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa
     4741 ggttaaagaa atcagagaat taaccactgc cgaaatgctt gatcaagaaa aacaattaaa
     4801 agaagaattg tttaatctta gattccaatt agcaacaggt caattagaaa acactgcacg
     4861 tattaaagaa gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg aacaagctaa
     4921 gtaattatgg aaggaggcca ttaagcgtat gactgaagga agaaatcaac gcaaagttta
     4981 tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt accgttgctg tcgaaacaaa
     5041 gaaaaaccac cctatctatg gcaaacgcat gaactattct aagaaataca aagtgcatga
     5101 tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt atggaaactc gtccattatc
     5161 agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa gcagttatta tctaataaca
     5221 cgagattttc ctatatagat tgaatgcaaa atctgaaagg aggatacaca acgtgatcca
     5281 agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa
     5341 agtccttggt ggatctggtc gtaaaactgc taacatcggt gatgtgatcg ttgctacggt
     5401 taaacaagca acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt
     5461 tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat attaaatttg atgaaaatgc
     5521 tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca cgtatctttg ggccagttgc
     5581 acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag aagttttata
     5641 atcttgagac acttatcaaa ggaggtgcga aacagttatg tttgttaaaa aaggcgataa
     5701 agttaaagtt atcactggaa aagacaaaaa caaagaaggc gttgtactag cagcgttccc
     5761 gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc
     5821 tcaagctgct cctcaaggtg gaattgttga gatggaagcg ccaattcatg tttctaacgt
     5881 gatggtggtt gactctactg gtgtagctgg ccgagttggc tacaaagaag tagacggcaa
     5941 aaaagtccgt gtttctaaaa aaaccggtga agttttagat aaataaaagt ttaaggaagg
     6001 agggtcttac tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt
     6061 gatggaaaaa tttaactata gctctgttat gcaaacacct aaagttgaaa aaatcgtcat
     6121 caacatgggt gttggtgatg cggtatcaaa cgctaaaaac ttagataaag cagttgaaga
     6181 attaactttg atcactggtc aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg
     6241 attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat
     6301 gtacgaattc ttagacaaat tggtttctgt ttcactacca cgtgtacgtg acttccatgg
     6361 tgtaagtaaa aaagcctttg acggtcgtgg aaactatact ttaggtatta aagaacaatt
     6421 gatcttccca gaagtagatt atgatttagt agataaagta cgtggtatgg acatcgttat
     6481 tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg ttgacacaat taggcatgcc
     6541 attccaaaaa taattaaagg aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa
     6601 caaacgtcct gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc
     6661 acattcagtt tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac ttgcctataa
     6721 aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc ataagaagga
     6781 ggtaaaagat caatggtcat gacagatcca attgcagatt ttctaactcg tatccgtaac
     6841 gcaaacatgg ttaaacatga gtctttagaa gtacctgcat caaaaattaa acgtgacatc
     6901 gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga agatgacaaa
     6961 caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac
     7021 ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca aagctgacga agtaccaaaa
     7081 gtattgaacg gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt cactgataaa
     7141 gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt acgtatggta atcaagtaga
     7201 atctacaagg aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg ttatccctga
     7261 aggagtaaca gttactcaag acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt
     7321 gactcgtact ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag taactttcac
     7381 tcgtccaaac gacagtaaag aaatgaaaac aattcatgga acaactcgtg cgaacttcaa
     7441 caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg
     7501 gtaccgtgca caattacaag gatcaaaact tgtattgaac gttggttact cacatccagt
     7561 agaaatcaca ccaccagctg gagtaactgt tgaagtacca gctaacacac aagtaattgt
     7621 taaaggtgcg aacaaagaag aagttggcga attagctgca aacattcgtg gaactcgtcc
     7681 tccagaacct tataaaggta aaggtattcg ttatgttggc gaatatgtac gccgtaaaga
     7741 aggtaaaact ggtaaataat cgtttagaaa aattgcatag aagccagcag caggaaaaag
     7801 gcatttggct gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc agcttctatt
     7861 tttcgaaacg tatttactgc ttgaaatagt agtacaataa aaataaagag gtgacaattg
     7921 tgattacaaa accagataag aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca
     7981 atatctctgg tactgctgag cgcccacgct tgaacgtttt tcgttctaac aaaaacatct
     8041 acgctcaaat tattgatgac gtagcgggtg taacgctagc aagtgcctct accttggata
     8101 aagaaatttc aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg
     8161 aacgtgcagc tgaaaaaggc attaaagtag tagtctttga ccgtggtgga tacctttacc
     8221 atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga
     8281 aggaggaata ccattcatgg tttatatcga tccaaaacat ttggaattag aagaccgcgt
     8341 tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac gttttgctgc
     8401 tttagttgtt gtcggtgaca aaaacggaca cgtagggttt ggtacaggta aagctcaaga
     8461 agttcctgaa gcaatccgta aagcaattga agatgcgaag aaaaacttag ttgaagtacc
     8521 tatggttggt tcaacaatcc cacacgaagt aatcggtgct tttagtggtg gtagaatcct
     8581 aatgaaacct gctgtagaag gttctggggt tgctgctggt ggaccagttc gtgccgtatt
     8641 agaattagct ggggtagcag atatcacttc taaatcttta ggctcaaata caccaatcaa
     8701 cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga
     8761 acttcgcggc aaatcagttg aagaattaat cggataagga ggacaacaat aatggctgaa
     8821 ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg cgatacagtt
     8881 aaagcgttag gtcttacaaa gattaacagt tcagtggtta agccttctaa tgaagcaatt
     8941 aaaggcatga tcaatactgt ttctcaccta gtggacgttg aagaaattta atttagaaaa
     9001 atcataacta gaacattaag gaggtgccaa acctatgaaa cttcatgaat taaaacctgc
     9061 tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa
     9121 aactgccgga cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg
     9181 atttgaaggg ggacaaacac cattgttccg tcgtttacca aaacgaggat tcacaaacgt
     9241 gaaccgtaaa gactatgcag tcattaatct tgatgtattg aaccgctttg aagacggagc
     9301 tgaagtaact cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat
     9361 caaagtatta gctaatgggg aactaaacaa gaaattaact gtgaaagcag caaaattctc
     9421 taaagcagct caagaagcaa ttgaagcggc tggtggatca atcgaggtga tctaatgttt
     9481 agactattaa aagatgcttt taaagtaaaa gacattagat cgaaaatcct atttactgta
     9541 tttgtgttgt ttgtatttcg cctaggtgca catattactg taccaggcgt aaatgctgct
     9601 tcattgcaaa acctttcttc tttgccattt ttcaacatgt tgaacttggt aagtggtagt
     9661 gcgatgcaaa acttttccat tttctcaatg ggggtttcac catacattac tgcttctatc
     9721 gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg
     9781 gaagttggtc gtaaaaaact gaatcaagcg actcgatatc tgacattagt attggcattt
     9841 atccaatctg tcggtatcac tgctggattc aatcaatacg ctcaattagg tttcgtgaat
     9901 aatcctaacg tagcaactta cgtaacgatc ggtttgattc taacagccgg tacgatgttt
     9961 gttacttggc taggagaaca gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc
    10021 atttttgcag gtatcatttc tagattgcct gaaagtatca gagaaattta tgaagattac
    10081 tttataaata ttgatccttc agacatatgg aaatctgcta tttttgtggc cattttacta
    10141 gtagcggtct tgttgatcgt gacattcgta acatttttcc aacaagctga acgcaagatc
    10201 ccgatccaat atacgaaacg cgtagctggc gcgcctacaa gcagctatct gccgttgaaa
    10261 gtaaatgcag ctggagttat tccagttatc tttgctagtt cgtttattgc tacgccaaat
    10321 gctgttttac aagcattgag cggtagattc ggttctgaaa actggtacaa agtgatgatg
    10381 gagatcttta actataatac agcacctggt gctacgattt atacgattct gatcgttgcc
    10441 ttcactttct tctacgcctt tgttcaagta aaccctgaga aattagcgga aaacttgcaa
    10501 aagcaaggaa gttatatacc aagcgtacgc cctggtaagg ggactgaaga gtatgtctca
    10561 agattactga tgcgtttaag tacagttggt gcagtcttcc ttggtttagt ggcattgctt
    10621 ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa tcggattagg cggtacaagc
    10681 ttactgatcg ttatcggtgt agctttagaa actgcgaaac agttagaagg cttaatgctg
    10741 aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca
    10801 cgttctcaca catttattag gagggaaaac aatgaacctc attttaatgg ggctgccagg
    10861 cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct tatggtattc cgcacatctc
    10921 aacaggagat atgttccgcg cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa
    10981 gtcttatatg gataaaggtg cattagttcc tgatgaagta acaaatggaa tcgtgaaaga
    11041 gcgactagct gaaccagata cagagaaagg cttcttatta gatggctttc cacgtacgct
    11101 agatcaagca aaagctctgg acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt
    11161 catcgatatc catgttggtg aagaaatctt ggtcgaacgt ctagccggcc gatttatctg
    11221 cagcaattgt ggagcaactt accataaaat tttcaaccct acaaaagttg aagacacatg
    11281 tgatcgttgc ggtgggcatg aattctatca aagagaagat gataaacctg agacggttaa
    11341 aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca
    11401 aggtttgcta aacactattg atggtgctcg tgaaatcgat gctgtatttg cagatgtaca
    11461 aaaaatcatt gaaaaatagc acgattgccc gtagccgact tgcattatgc agttactctt
    11521 gtcaagttca ataaattatg atagaatatg tcagtccgac ttctagagaa tcctctagat
    11581 gatccatttt gagaattttg aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa
    11641 tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag
    11701 gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc
    11761 aagttcttgc tactgtttca ggtaaaattc gtatgcatta catccgtatc ttacctggtg
    11821 acaaagtaac ggttgaattg tctccatacg atcttaaccg tggccgtatt acttatcgct
    11881 ttaaataatt gtactccgta aaactacagg aggtattatc atgaaagtaa gaccatcagt
    11941 aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg
    12001 cccagcaaat ccaaaacata aacaacgtca aggataattg gaggtgctat aaatatggct
    12061 cgtattgcag gagtagatat tcctcgtgat aaacgtgtag tagtttcact tacttatatc
    12121 tatggtattg gtaacactac tgctaaaaag atcttagctg atgctggtgt atctgaagat
    12181 gttcgcgttc gtgaattaac gaatgaacaa acagatgcta tccgtgcaga agtagataaa
    12241 ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa
    12301 atcggttctt accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg
    12361 aaaaacaatg cacgtactcg taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa
    12421 ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt
    12481 gtgaagaaga atatagaagc aggtattgca catatccatt ctacattcaa taacactatc
    12541 atcatgatca ctgatactca cggaaatgca ttagcgtggt catcagctgg ttcattaggt
    12601 ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga aactgctaca
    12661 aaagcagcaa tggaacacgg attaaagact gttgaagtaa cagttaaagg acctggttct
    12721 ggacgtgaag cagcaattcg ttcattacaa gctacaggtt tagaagtgac tgcaattcgt
    12781 gacgtgactc cagttcctca taatggtgcc cgccctccaa aacgccgtcg tgtttaatga
    12841 gtgccgctca ttttgagttt tatcagtaac gtcattgaac aagacacttt tcgttttgaa
    12901 aggggtaaag ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga
    12961 aaaagattat ggcaagttca tcgttgaacc tcttgaaaga gggtatggga ctactttagg
    13021 gaattcccta cgtcgtattt tattatcttc tttgccaggt gctgccatca ctaatattca
    13081 aatcgatggt gtactgcatg aattctctac catcaaaggt gttagagaag acgtcactca
    13141 aatcatcttg aacattaaag gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct
    13201 tgaaatcgat attaccggac ctgctacagt aactgctggc gatattatcg ttgatagtga
    13261 tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc tctgaaggag ctacgttcca
    13321 tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga
    13381 agatatgcca attggcgttc ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa
    13441 ctatcaagtt gagaacacac gtgttggaca ccgtgaagat tttgataaat tgacgatgga
    13501 gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat
    13561 gactgaacat ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat
    13621 ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg aagagttaga
    13681 cttgtctgtt cgctcttaca attgtttgaa acgtgcagga atcaatactg tacaagaact
    13741 tacaaataaa tctgagcctg aaatgatcaa agtccgtaac ttaggacgta aatcattaga
    13801 agaagttaaa gcaaaattgc acgacttagg tttaggttta cgtaaagaag attaaaacct
    13861 tttacgaagg agggaaacca acgtgagtta tcgtaaacta ggacgcacat ctagccaacg
    13921 taaagcgatg ttgcgtgatt taacaactga tttattaatc aacgaacgta ttgtgactac
    13981 tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg
    14041 cggagatttg catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt
    14101 gcgtgaagaa aatgaggaca tcgtcattga atcagcttta caaaaactgt tcaacgatat
    14161 tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc attttgaaga cagaaccaag
    14221 acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca ctaataacat
    14281 cactttatag atgattcttt tagagtgtta tgatgttgga agatattttt ccgagtctag
    14341 ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa gcactcatca taaagtgttt
    14401 ttttatacct tcaattacct gtcatgacag atatgtttga aggcataaaa aaacatttta
    14461 agcgagaaat aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg
    14521 aagaaggagt aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc
    14581 cagatgatct tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga
    14641 tcgctattat tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat
    14701 tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata
    14761 tttggacgat ccgacagatg gttggaatga tttttcaaaa tccagacaat cagtttgttg
    14821 gttcgacagt agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag
    14881 aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac
    14941 gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg
    15001 ccttacgacc tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc
    15061 gtgaagaagt gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat
    15121 ctatcactca tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag
    15181 gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg
    15241 acttaggtct agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg
    15301 ttgatgtgcc cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg
    15361 ttttgaacaa gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt
    15421 tgacatcaat atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag
    15481 cgggaaatca accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt
    15541 ccatattgga gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg
    15601 aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc
    15661 aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct
    15721 agcaaaagaa acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt
    15781 tgaactttca ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc
    15841 ggaagtactt gtattggatg aaccaacggc aggacttgat ccccagggca gaaaagaaat
    15901 gatggagatg ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca
    15961 tttaatggat gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat
    16021 cgttaatagc ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca
    16081 actaggcgta cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt
    16141 tgctaccttg cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg
    16201 aaatgcccat gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc
    16261 atcgattgga tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt
    16321 tagcaaataa ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct
    16381 tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc
    16441 tcttcactgt agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg
    16501 gtgttttcca tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg
    16561 tgttgatcat ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg
    16621 atgcgattga gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt
    16681 cattgatgct gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga
    16741 tcatgaacgc tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa
    16801 tgaaagcagt cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag
    16861 atcttgcgac tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc
    16921 gtgtattgca ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg
    16981 tgctactgat ctttttaaga ggataaacgt tcagtgggcc gctatctatt cttttcttgc
    17041 tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa gaaatattag ggtctgtggc
    17101 agactaaggt cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa aattttatag
    17161 aaaaatcatt attccagata accttttagg aacaaaggag agaaaaacat ggtacgttac
    17221 aaagcaatca ttgcgtacga cggaacaaat tttaatggat tccaaaaaca gccaaatggg
    17281 cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc
    17341 acggtctttg gttcgggaag gacagatgcc ggagtacatg cgatcgggca agtaatccat
    17401 tttgattatc cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca
    17461 ccagaggata ttgctgtaag acaagtagaa attgtttcag aagacttcca cgcaagatat
    17521 ttggtcaaag aaaagacgta tcaatttcga gtggatatag ggaaaccacg cagtcctttt
    17581 cgacgccatt atgcaagtta ttttccttac ccacttgatt tggaaaaaat tcagcgggcg
    17641 cttcctgatt tattagggac tcacgatttc acctcttttt gtgcatcagg aagctcgata
    17701 gaggataaaa tacgtacgat ctacgaggct aagatggagg taaatgaaac aaaagacgaa
    17761 ttgttgttta catttagggg aaatggcttc ttgtataaga tgatacggat cttagtagga
    17821 acgcttttga aaattggcaa tggccgattg cctgaagata gtatcccaga tattattgcg
    17881 aaaaaagatc gtaatgcagc aggacctaca gcacaccctg aaggacttta tttgtatgaa
    17941 gtgaaatatg aataagaggt tatgatagaa gtggtcagct tcaagaaata agaaggtatt
    18001 tacgaaaatt acttctcaaa attttgcaaa atttcggctt attttcgaag aagctacttc
    18061 tggaacaccg tttaagaaag aggtcgtgag cctgtcattt aactctgagt cacaaagagc
    18121 aatttcaaaa aacagcttct catctttgat gaatatcgtg acttgtggac gaggcgttga
    18181 taagtgaatc atctttttgt tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt
    18241 tttgattgcc cttggtgcgg acagtctgaa caggttccat cgtttcttta ttttctacgt
    18301 cttcttctgt gtgtgtttcg tggttgttct tctattgctg gatcaatcgt gccataaaag
    18361 gggcgattgc ggtcaaggta ccagacataa agaataaaca atgcaataca taggacaaaa
    18421 aggattgcac ctatcgtaaa tccttcaggc aagtaactga aagtgacagt atgtttacct
    18481 gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac tggtttccca
    18541 tcaattttag ctttccatcc tttgtcataa ggaatagttg tcaccaacat ggaatcttca
    18601 gcggtattta cagtcccaga agcttttctg ccagtggctt ccatttcgac acctttttct
    18661 ttgatctgat tcattgcggc ttgataggct acaacatcta gaccaacgac tttaggctcc
    18721 atgaaactta tttctttcgt cccataaaaa cttgcagtaa atgtcactgt tgtctcttga
    18781 ggataatagc caagatcata gtattgacca gaaatgttga tttgcgtttt tcgcgatgta
    18841 ccattgacag tgatcgtcgc actagaactt tctaattgcg caaaattggt tgggaacaaa
    18901 cttaagtaag cttgtgtatt tgcaggcaca tggacttccc aagtgagctc ttttggctgg
    18961 ttatctgctg tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt cgtattcaac
    19021 tgattcttca aagtaggtgt atagaatgta aaatactgtt catttagacc agacaatgta
    19081 ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg gctgctcaac atcattaacg
    19141 gacaacggtg cagtaaatgc tagtggcaat gcattgacat tctgatacag tgtatagtcg
    19201 ccgctttgac cagcttggcg gaagccatat ttactgaacg tgctttcgtt tttagaaatg
    19261 ttatatttga ttcctgtaaa cccatccatc agcaaagtat tattgttata acggatattc
    19321 aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt aagaggaaga attacgattt
    19381 cttatagaag aaaacaaact gataccactg taaccatagt tgatcgaatc gtttgcagat
    19441 actggatcca gattttccaa gcggtaaaac gagtcggaat ctttttttgt ttcatttaca
    19501 agagctttca agtcaggata aggatcagta tataaactgc gggaagcata attccaatca
    19561 tcaagaatgc catggatcat actgttcgta ttgaccagtg cttcagcaga catgatcaat
    19621 aataacaata tactcagatg attgaatgaa agttttttat gctgataaaa tcccacagca
    19681 aaagcataaa tccctaagaa aaccactgtc aatacaaagg aagtcatcgt cacataggtg
    19741 tagcttcctt caggtttgat gccccatgcc accgcaaaga gagcagctac gaccaaaatg
    19801 attcctaaca acagcccacc atcgtcttgt tctatctttt cccatccttt tcctgcaagc
    19861 aggagcacta agaaagagaa cagataggca taacggaata aaaacatatt cggtgcatgc
    19921 ataccatgcc agaacaaatt caatggaaca agataaaaac ttgcgataag tatggcgaaa
    19981 agactgccgt acaaaaattt atttttcaac ggtactttcc gactggcaaa ataaaacaga
    20041 cagaggacta atggtagcag acctgcgtaa acgaacggaa tcgatccgta ttttgtcgtg
    20101 tcataaacac caatcatatt tttcatgatt agatccagat aagaagtcgc ttctgttttc
    20161 aacgtagtga tctgactcag tgcctcgcca tttgaccgaa gatccagcac tgctggcaat
    20221 acaatgatca ttgaagcgcc gcctgccagt aaagaagtga taccataagg aacaatcgtt
    20281 tttttatatt ctgaccagtt tgtcaataga cgggcgataa aatataagaa agaaaatacg
    20341 ccaatcataa aacccatata aaaactggat aaaaacaaga gtaaataact gataaataaa
    20401 agtttcggcc gtttaagatc cataacacga tgaatcccca aaatcactaa tggaagataa
    20461 acaaatgcat ccagccacat gatgatctcc gaatgtgcag tgataaatga gatcaatgcg
    20521 taactgacgc taagtgcaac atgcccccag cgaggaatct tgaacgtacg tgcaagaaac
    20581 caaaaagcca atccggcaca gccgacctta agcaaagtca gaacatacag tgcatctggc
    20641 atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag atagtaagaa
    20701 attaaagata aataattcaa tcctagagaa gcattccatg tataaaaaat actttgcttt
    20761 ccaagaagaa catttcggaa gctggcatga aaattagaaa actgtgaaaa ggcatcactt
    20821 gccaaaatac ttctgctgct tccaggatag attccgatac ttagataaac aagtgctaaa
    20881 atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc cgttttctct aaagaatgac
    20941 tttaaatttt ttttcatatt tattttccac caaatttcta tgaagttgta ttcagtttat
    21001 catatccttg tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag
    21061 aataaataac ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat
    21121 caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc
    21181 caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc
    21241 caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca
    21301 gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct
    21361 gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga
    21421 ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt
    21481 catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa
    21541 ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata
    21601 aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt
    21661 aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt
    21721 tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa
    21781 agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca
    21841 agaagataat ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc
    21901 tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt
    21961 aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg
    22021 agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga
    22081 aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa
    22141 tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa
    22201 tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt
    22261 aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg
    22321 cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt
    22381 aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt
    22441 aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga
    22501 aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc
    22561 aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt
    22621 agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg
    22681 agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa
    22741 tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta
    22801 ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta
    22861 cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga
    22921 cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca
    22981 aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat
    23041 tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta
    23101 tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc
    23161 taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc
    23221 gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat
    23281 ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac
    23341 gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa
    23401 caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat
    23461 cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga
    23521 tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca
    23581 aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga
    23641 acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat
    23701 ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca
    23761 gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga
    23821 tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat
    23881 gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg
    23941 ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag
    24001 gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc
    24061 tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg
    24121 caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc
    24181 attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac
    24241 gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac
    24301 aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc
    24361 acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga
    24421 gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa
    24481 cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa
    24541 gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa
    24601 tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc
    24661 tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc
    24721 ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt
    24781 gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg
    24841 tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga
    24901 cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca
    24961 ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac
    25021 cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta
    25081 tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat
    25141 gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga
    25201 acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa
    25261 atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg
    25321 gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat
    25381 ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg
    25441 cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc
    25501 atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt
    25561 gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta
    25621 ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa
    25681 gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa
    25741 tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct
    25801 gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa
    25861 agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt
    25921 atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga
    25981 gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa
    26041 gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa
    26101 aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca
    26161 attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa
    26221 gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc
    26281 gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg
    26341 aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa
    26401 catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga
    26461 cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg
    26521 aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa
    26581 gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac
    26641 gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct
    26701 attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt
    26761 tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa
    26821 gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc
    26881 caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa
    26941 agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat
    27001 tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt
    27061 ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa
    27121 tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa
    27181 agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga
    27241 aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag
    27301 cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt
    27361 ttatccttct ttttttaatc tttttgacac gtaaaagtca tttttctgat aattctggcg
    27421 ataaaca
//
LOCUS       DAHUWM010000011        26604 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHUWM010000011 DAHUWM010000000
VERSION     DAHUWM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.011"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1152
                     /locus_tag="NK788_000325"
     CDS             <1..1152
                     /locus_tag="NK788_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCH6768704.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="NK788_000326"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="NK788_000326"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCH6768705.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="NK788_000327"
     CDS             2728..3003
                     /locus_tag="NK788_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCH6768706.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="NK788_000328"
     CDS             3009..3776
                     /locus_tag="NK788_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCH6768707.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="NK788_000329"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="NK788_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCH6768708.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="NK788_000330"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="NK788_000330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCH6768709.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="NK788_000331"
     CDS             7920..8222
                     /locus_tag="NK788_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCH6768710.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="NK788_000332"
     CDS             8225..9388
                     /locus_tag="NK788_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCH6768711.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="NK788_000333"
     CDS             9414..12842
                     /locus_tag="NK788_000333"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCH6768712.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="NK788_000334"
     CDS             12863..13987
                     /locus_tag="NK788_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768713.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="NK788_000335"
     CDS             13977..14414
                     /locus_tag="NK788_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCH6768714.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="NK788_000336"
     CDS             14398..14736
                     /locus_tag="NK788_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCH6768715.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="NK788_000337"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="NK788_000337"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCH6768716.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="NK788_000338"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="NK788_000338"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCH6768717.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="NK788_000339"
     CDS             15893..16936
                     /locus_tag="NK788_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCH6768718.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="NK788_000340"
     CDS             17095..17823
                     /locus_tag="NK788_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCH6768719.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="NK788_000341"
     CDS             17939..18184
                     /locus_tag="NK788_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCH6768720.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="NK788_000342"
     CDS             18181..18399
                     /locus_tag="NK788_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCH6768721.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="NK788_000343"
     CDS             18535..19074
                     /locus_tag="NK788_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCH6768722.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="NK788_000344"
     CDS             19067..21340
                     /locus_tag="NK788_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCH6768723.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="NK788_000345"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="NK788_000345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCH6768724.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="NK788_000346"
     CDS             complement(22513..22767)
                     /locus_tag="NK788_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCH6768725.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="NK788_000347"
     CDS             complement(23167..23436)
                     /locus_tag="NK788_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768726.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="NK788_000348"
     CDS             complement(23906..24361)
                     /locus_tag="NK788_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCH6768727.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="NK788_000349"
     CDS             complement(24499..26427)
                     /locus_tag="NK788_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCH6768728.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8860 a         4610 c         5614 g         7520 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taag
//
LOCUS       DAHUWM010000012        26421 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHUWM010000012 DAHUWM010000000
VERSION     DAHUWM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.012"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            3..191
                     /locus_tag="NK788_000350"
     CDS             3..191
                     /locus_tag="NK788_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCH6768729.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(249..602)
                     /locus_tag="NK788_000351"
     CDS             complement(249..602)
                     /locus_tag="NK788_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768730.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1133..1555
                     /locus_tag="NK788_000352"
     CDS             1133..1555
                     /locus_tag="NK788_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6768731.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1552..1782
                     /locus_tag="NK788_000353"
     CDS             1552..1782
                     /locus_tag="NK788_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768732.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2280..2480
                     /locus_tag="NK788_000354"
     CDS             2280..2480
                     /locus_tag="NK788_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCH6768733.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2507..3700
                     /locus_tag="NK788_000355"
     CDS             2507..3700
                     /locus_tag="NK788_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6768734.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3768..5333)
                     /gene="guaA"
                     /locus_tag="NK788_000356"
     CDS             complement(3768..5333)
                     /gene="guaA"
                     /locus_tag="NK788_000356"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCH6768735.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5392..6315)
                     /locus_tag="NK788_000357"
     CDS             complement(5392..6315)
                     /locus_tag="NK788_000357"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCH6768736.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6511..7116
                     /locus_tag="NK788_000358"
     CDS             6511..7116
                     /locus_tag="NK788_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6768737.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7424..8440
                     /locus_tag="NK788_000359"
     CDS             7424..8440
                     /locus_tag="NK788_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCH6768738.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8581..9882
                     /locus_tag="NK788_000360"
     CDS             8581..9882
                     /locus_tag="NK788_000360"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCH6768739.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9928..10590
                     /gene="deoC"
                     /locus_tag="NK788_000361"
     CDS             9928..10590
                     /gene="deoC"
                     /locus_tag="NK788_000361"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCH6768740.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10611..11009
                     /locus_tag="NK788_000362"
     CDS             10611..11009
                     /locus_tag="NK788_000362"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCH6768741.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11075..12157
                     /locus_tag="NK788_000363"
     CDS             11075..12157
                     /locus_tag="NK788_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6768742.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12369..13931
                     /locus_tag="NK788_000364"
     CDS             12369..13931
                     /locus_tag="NK788_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768743.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13924..15057
                     /locus_tag="NK788_000365"
     CDS             13924..15057
                     /locus_tag="NK788_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6768744.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15057..16013
                     /locus_tag="NK788_000366"
     CDS             15057..16013
                     /locus_tag="NK788_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6768745.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16112..17278
                     /gene="deoB"
                     /locus_tag="NK788_000367"
     CDS             16112..17278
                     /gene="deoB"
                     /locus_tag="NK788_000367"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCH6768746.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17302..18120
                     /locus_tag="NK788_000368"
     CDS             17302..18120
                     /locus_tag="NK788_000368"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCH6768747.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18137..18844
                     /gene="deoD"
                     /locus_tag="NK788_000369"
     CDS             18137..18844
                     /gene="deoD"
                     /locus_tag="NK788_000369"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCH6768748.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19142..19828)
                     /gene="gpmA"
                     /locus_tag="NK788_000370"
     CDS             complement(19142..19828)
                     /gene="gpmA"
                     /locus_tag="NK788_000370"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCH6768749.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20000..20581)
                     /locus_tag="NK788_000371"
     CDS             complement(20000..20581)
                     /locus_tag="NK788_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6768750.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(20714..21397)
                     /gene="rpiA"
                     /locus_tag="NK788_000372"
     CDS             complement(20714..21397)
                     /gene="rpiA"
                     /locus_tag="NK788_000372"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCH6768751.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21716..22129
                     /gene="rpsL"
                     /locus_tag="NK788_000373"
     CDS             21716..22129
                     /gene="rpsL"
                     /locus_tag="NK788_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCH6768752.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22213..22683
                     /gene="rpsG"
                     /locus_tag="NK788_000374"
     CDS             22213..22683
                     /gene="rpsG"
                     /locus_tag="NK788_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCH6768753.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22777..24864
                     /gene="fusA"
                     /locus_tag="NK788_000375"
     CDS             22777..24864
                     /gene="fusA"
                     /locus_tag="NK788_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCH6768754.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25036..26223
                     /gene="tuf"
                     /locus_tag="NK788_000376"
     CDS             25036..26223
                     /gene="tuf"
                     /locus_tag="NK788_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCH6768755.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8320 a         4887 c         5605 g         7609 t
ORIGIN      
        1 ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca agactacgaa
       61 tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa
      121 ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac gccaagacgc
      181 agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt ttatctgaat
      241 tttactgact agccttcaat atttcttgct tcattgatac cttttgctag tgcttccatt
      301 aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa ccttcgagtg
      361 cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg gagtaatgat
      421 acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat atttgttaaa
      481 taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc ccgtttcatt
      541 cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc ttttttacgc
      601 atactaaatc acctctatac attttactgt tcctatataa ccataaacag gtatagaaaa
      661 acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa tgcgatgaat
      721 ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat aaaatttagt
      781 catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc gccatgagaa
      841 agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt atccacctgt
      901 actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa aaatcaaagc
      961 atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata aatatctttt
     1021 aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta gtatgaaggg
     1081 agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa gcatgaaacc
     1141 atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac aagttattga
     1201 agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag aagtctcgtt
     1261 ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt acgtcactga
     1321 tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt tattaagtga
     1381 agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt actttatgga
     1441 tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg tttatcgtca
     1501 tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag aatgaaaaca
     1561 gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga cgtggaagca
     1621 attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt acgtcgcatg
     1681 acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg cagaatggaa
     1741 acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt tttcgtggaa
     1801 gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca tcaaagcata
     1861 ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac aaccaaataa
     1921 aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc atgacctaca
     1981 aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga ttgctgccat
     2041 gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt tagaagcgtc
     2101 acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg tgagctttgc
     2161 tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat aaaactattt
     2221 gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa ggagtaataa
     2281 tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct ttttcagcag
     2341 acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac tatcaatctc
     2401 gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt cggttttcaa
     2461 atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg ttaaagaccc
     2521 aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt atgattctaa
     2581 agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag caagagaaga
     2641 atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg ataaaatgac
     2701 gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg taaagctcag
     2761 aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg ataaaatggc
     2821 tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat caaagaagtg
     2881 taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg atcgagcaat
     2941 tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga aaaagcaaaa
     3001 gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc taatttacaa
     3061 agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta tgactggaat
     3121 gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact caggagtact
     3181 aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg ttgaaccaaa
     3241 aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt atttatccga
     3301 atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct ataacggtat
     3361 tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc tggctggtat
     3421 ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa tcagtatggg
     3481 agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga cgactttagg
     3541 aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat taaatggagc
     3601 catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc aatttactgt
     3661 agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta aaaagataag
     3721 aagagccaga aacccttggt ataagaggat ttctagctct cctacaacta ttcccactcc
     3781 actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg ggctacttcg
     3841 tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc aaagtcagcg
     3901 gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta agtacgtccg
     3961 tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg ccagatatca
     4021 cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc gcgtacgatt
     4081 tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg accagggaat
     4141 ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc acggacttcg
     4201 tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc tggcagtcca
     4261 cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc gataacgtca
     4321 gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag ttttgttgct
     4381 tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg ttctgggtca
     4441 gatacacctt tcaacttgtc taagaagcga tctcttgcat cgactttgat gatgttcaag
     4501 ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg tagcagaccg
     4561 tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag aaccccaacg
     4621 acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc aacttgttca
     4681 cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc ttcacagtga
     4741 caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg acgtacttct
     4801 gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc aatcggacaa
     4861 tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg
     4921 ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg atcgcttgtt
     4981 acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga accgccaagt
     5041 ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc taaattgaag
     5101 atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc gccagaaaga
     5161 atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg gctcaataat
     5221 tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata ctgactaccg
     5281 tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt caaatttatt
     5341 acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat gctaatattt
     5401 tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta tatcggctct
     5461 tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt tccatacatc
     5521 ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat aataattctc
     5581 tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc atttttcgat
     5641 cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga cgtagatttg
     5701 ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat caggctgctg
     5761 gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa caggtacttt
     5821 tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct ccatatcata
     5881 actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac gatttggata
     5941 aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat
     6001 cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg cgatcccgat
     6061 aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca agaacaaaga
     6121 ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga tatctcgaac
     6181 atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat cttgtgtcaa
     6241 aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac ggtagtagtt
     6301 cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat tttaacacaa
     6361 aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata atctttataa
     6421 tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg atagactgtt
     6481 gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc tgaacgccca
     6541 gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac ctttcaattt
     6601 gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg tgtcctgatt
     6661 gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt gggatgcggg
     6721 tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga aatggtcgac
     6781 attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat
     6841 gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta tgcagctatc
     6901 gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt gacaggcgca
     6961 tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa acaaggagcg
     7021 ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat
     7081 aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata gtggagcgac
     7141 gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt tgttgagaag
     7201 tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt
     7261 tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa gtcggtagat
     7321 aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt cttgggggtt
     7381 ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc tgaccatcag
     7441 agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt tgaataacaa
     7501 aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga atgaacatca
     7561 ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga ccatcggaat
     7621 gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg tagagagcta
     7681 tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc aagaaaatga
     7741 gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc tggccacacc
     7801 aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa tgccaatcat
     7861 tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa aggaatatga
     7921 gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga tcggcatgct
     7981 gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc gtcatgcgct
     8041 gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt tgactgtcca
     8101 aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg ctattttttg
     8161 cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat
     8221 cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat acatgattcc
     8281 gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg cacgtttcct
     8341 gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt
     8401 aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta tattgcttat
     8461 gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca gtttactaaa
     8521 gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga ggatcaggca
     8581 atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa
     8641 atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca agtcagtgca
     8701 ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa cttaacattg
     8761 gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat
     8821 aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc cctagtagct
     8881 agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac aggtggtaca
     8941 ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga agaatttgta
     9001 cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt ggcaccggcg
     9061 gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct tccgctgatt
     9121 gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg
     9181 accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct ggcaaagacc
     9241 atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc agatatgtca
     9301 gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat cgaaacatta
     9361 caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag ccaaatggtt
     9421 gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca agaagctctg
     9481 gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca
     9541 gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga gcttcctgct
     9601 aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg
     9661 ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg attgaaactg
     9721 cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac
     9781 gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat tggtgaatcg
     9841 gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa agcggacttt
     9901 accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga ccatactatt
     9961 ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac
    10021 catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga acaattacaa
    10081 ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa cacacccgag
    10141 acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt cgatatggtc
    10201 atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga tatcgaagca
    10261 gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta
    10321 gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc
    10381 aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt
    10441 gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa
    10501 gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg agtagcaatc
    10561 gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc atggcagaac
    10621 tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat gttccttatt
    10681 cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat caaggcatca
    10741 acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct tttttcaaag
    10801 cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac acccccgatc
    10861 ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc cctgatatgc
    10921 cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc
    10981 cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct gactgttgaa
    11041 aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat tatttggttt
    11101 aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca acggctctac
    11161 tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga tcacagacac
    11221 aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag aaaaatgggg
    11281 gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat cttcaaatga
    11341 atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa cgatttttgg
    11401 tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata caggtacgaa
    11461 ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg caactttcaa
    11521 agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt
    11581 agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg ctggattcaa
    11641 agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata
    11701 tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta tgtatgcaca
    11761 aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat tccaagaagc
    11821 aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg accgcgacca
    11881 atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat tgacatctac
    11941 actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg acggtaaatt
    12001 ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc taacagaagg
    12061 acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg
    12121 tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata gccttgttag
    12181 acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt atccaagctg
    12241 ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt caaaagaaga
    12301 acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg ttaggatgtg
    12361 aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc aaagaatttg
    12421 gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt
    12481 tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc ggattattag
    12541 agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca
    12601 aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt gatgccttta
    12661 ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg cttgaccata
    12721 aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca gtcaacccag
    12781 atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac
    12841 tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg acccctcaag
    12901 aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag tcgatcattt
    12961 tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca gttatccgcc
    13021 gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa ttagccgata
    13081 tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag
    13141 tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa
    13201 acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc gatggaaacg
    13261 ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc ggacatatca
    13321 agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa catggtgtag
    13381 gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca ttatctgaaa
    13441 atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg ctgaattatt
    13501 cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga acaacgaatg
    13561 aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg
    13621 aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag
    13681 gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt
    13741 tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt gctgttatcc
    13801 atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat gaattaggtc
    13861 tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag ggggctggcg
    13921 cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact ttcagttgtt
    13981 ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc catccaaggt
    14041 tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga aacgttaagg
    14101 caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc agctgggttt
    14161 ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca
    14221 cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc
    14281 ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt tggaacaagt
    14341 gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac atttgcttta
    14401 catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat gatcagtcaa
    14461 aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg cgtaaactca
    14521 ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact
    14581 ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta tgcaggaatg
    14641 agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc agggcttggc
    14701 ggtgtggttg aaggattagg aacgtatcaa aatttctttg ttcaaacgac ttcgctatct
    14761 attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat cggtattctt
    14821 ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca gacaggagca
    14881 ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt tgtcgggatc
    14941 aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca agaagagatc
    15001 gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga actataatgt
    15061 cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca acaccgctaa
    15121 ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac gtagggctag
    15181 agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg atgagtccat
    15241 cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta ggtatcctat
    15301 tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt atcagcggga
    15361 cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca tggtatggta
    15421 aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca ggattagcag
    15481 atattccggt cattggacca attttcttta aaaatacatt cttacctgct tattgtgcta
    15541 ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc
    15601 gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta tatgctatgc
    15661 gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct gtttttgctc
    15721 agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc atctccatgg
    15781 ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca ttgttctttg
    15841 gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct tcgattccgc
    15901 cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt gttttcttag
    15961 ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa taatctttta
    16021 gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt gttttaaagg
    16081 caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc tgatcgtcat
    16141 ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg ttggttctga
    16201 tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag agcagcttgg
    16261 tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc
    16321 gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact gggaaatcat
    16381 gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg atgatctgat
    16441 caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt atagcggaac
    16501 agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac
    16561 ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt tagaagaatt
    16621 atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata tgattggccg
    16681 aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa gcaatcgcca
    16741 tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag aagcaggaaa
    16801 ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa tcactgaatc
    16861 tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga
    16921 tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg gtcatcgtcg
    16981 ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc cagaattgct
    17041 tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa acgatccgac
    17101 gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat
    17161 gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa cgattgctga
    17221 gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc
    17281 tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga aacgacccag
    17341 tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg ttcaggatta
    17401 ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga gatcccgttc
    17461 tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc tctggcaggc
    17521 aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc catgcaaaca
    17581 gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat tgtgactaat
    17641 gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat taccgatcat
    17701 atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat gggccctaga
    17761 tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa agcagcagca
    17821 gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg accaacttat
    17881 gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt agggatgtcg
    17941 actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg aatctcttgt
    18001 attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca
    18061 actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac attattgtaa
    18121 taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa
    18181 gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt ttttggaaga
    18241 tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat ataaaggaga
    18301 acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct atgcccatga
    18361 attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg gtgcattgtc
    18421 aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca gttcttcgat
    18481 gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca atctattact
    18541 gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa atgttctttc
    18601 ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg aacttggcgt
    18661 tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat accatgtgca
    18721 gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa caacagctgc
    18781 agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag cgatagcaga
    18841 ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca agcaaaaata
    18901 gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt ttgaacaccg
    18961 tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca atttcaaaaa
    19021 acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc aggagaaaac
    19081 cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt tatagtgttt
    19141 tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa acaagtggtt
    19201 gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg atatgtttag
    19261 ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg tctagtaatg
    19321 ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact ttcaagtttt
    19381 ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt gctgcagaac
    19441 cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc cagatgtgca
    19501 cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt aatttaccat
    19561 agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga tctgaatatt
    19621 ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa gcaacatcaa
    19681 attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg attccttctg
    19741 gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat tcactaagac
    19801 cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata ggtttatatt
    19861 tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc catcgaaaaa
    19921 ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca gtgcccacgc
    19981 aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca ggtaaattga
    20041 tatatttata acatgttcca actgaaacac cgcattttga agaaataaat tggatcgttt
    20101 cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg atttttggtc
    20161 ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta cgttctttga
    20221 tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt tttcccattt
    20281 cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct ttgaataatt
    20341 gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg caaatgacta
    20401 gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca gtagaatcat
    20461 tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca gataattgat
    20521 caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca ataattttca
    20581 tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa tgtagcataa
    20641 agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta ttggatgatc
    20701 aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct ttttctgagc
    20761 cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc ccaacaagtt
    20821 catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc agatcaatga
    20881 tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc aagacgggtt
    20941 ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg atcacttcaa
    21001 ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg atccaaatcg
    21061 tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg ccgcctttga
    21121 tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg actacgggga
    21181 cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt gtcgcatttg
    21241 aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca atctcttcta
    21301 ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc tttacgaatt
    21361 tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc tttttttagt
    21421 ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga taccattctt
    21481 ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat gttttttcat
    21541 tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac gtgctgactg
    21601 tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat cacaaaaaaa
    21661 gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac aaggaatgcc
    21721 tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc
    21781 tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa actcacctca
    21841 aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga actcagcttt
    21901 acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt atatcccagg
    21961 gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga
    22021 cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg gtgttaacga
    22081 ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat tttaattatt
    22141 aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat atttcggaag
    22201 gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt accagatcct
    22261 atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga cggaaaacgc
    22321 gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc tacaggtaac
    22381 gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct tgaagttaaa
    22441 gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc tgaacgccgc
    22501 gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga acacacaatg
    22561 gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc ttctgttaaa
    22621 aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca ctatcgctgg
    22681 taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt ccttattttt
    22741 agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt ttctttacaa
    22801 aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac tgctactgaa
    22861 cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga aggagcttca
    22921 caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc tgctgctact
    22981 acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca cgtagacttc
    23041 acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt tcttgatgca
    23101 caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac ttatggtgta
    23161 cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt atattcagta
    23221 tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc aattggtgcg
    23281 gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat gtatacaaac
    23341 gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga attagctgaa
    23401 gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact tactttgaaa
    23461 tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg ccgcgcaaca
    23521 gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa aggggttcaa
    23581 ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc agctatcaaa
    23641 gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag cgctcctttc
    23701 tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac tttcttccgt
    23761 gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt
    23821 gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat ctctgaagtt
    23881 tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg ggacacacta
    23941 tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc agttatccaa
    24001 gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc tttacaaaaa
    24061 ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc
    24121 atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg tcgtgaattc
    24181 aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc
    24241 actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata cggtcacgta
    24301 tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc
    24361 ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa agatgctatg
    24421 gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact ttacgatggt
    24481 tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc tatggcatta
    24541 cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc tgtagaagtt
    24601 gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg tcgtggacgt
    24661 gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat ggttccactt
    24721 gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg tggtacattt
    24781 acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga aatcattaag
    24841 aaaaacggcg gaaaagctga ataatttctt aaaaccggat agtcctgcaa agagattatc
    24901 caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga tatgggcatg
    24961 tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat tgtattttta
    25021 ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt
    25081 ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat cacaactgta
    25141 ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg tgctccagaa
    25201 gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac agatacacgc
    25261 cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat gatcactggt
    25321 gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc aatgcctcaa
    25381 actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg
    25441 aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat ggaagttcgt
    25501 gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc tggatcagct
    25561 ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca
    25621 gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat gatgccagtt
    25681 gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt tgaacgtgga
    25741 caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca
    25801 acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc tggagacaac
    25861 attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca agttttagct
    25921 aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa
    25981 gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt ctacttccgt
    26041 acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc
    26101 gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa
    26161 ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga aatcaaagct
    26221 taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa
    26281 ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa aaaagacagg
    26341 agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa tgaaacttta
    26401 tgaaaacaaa atgtttttta c
//
LOCUS       DAHUWM010000013        26414 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHUWM010000013 DAHUWM010000000
VERSION     DAHUWM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.013"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(3..215)
                     /locus_tag="NK788_000377"
     CDS             complement(3..215)
                     /locus_tag="NK788_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768756.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            402..869
                     /locus_tag="NK788_000378"
     CDS             402..869
                     /locus_tag="NK788_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768757.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            866..1108
                     /locus_tag="NK788_000379"
     CDS             866..1108
                     /locus_tag="NK788_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCH6768758.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            1126..1428
                     /locus_tag="NK788_000380"
     CDS             1126..1428
                     /locus_tag="NK788_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768759.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1499..3553
                     /locus_tag="NK788_000381"
     CDS             1499..3553
                     /locus_tag="NK788_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768760.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            3553..5385
                     /locus_tag="NK788_000382"
     CDS             3553..5385
                     /locus_tag="NK788_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCH6768761.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            6098..7996
                     /gene="ltrA"
                     /locus_tag="NK788_000383"
     CDS             6098..7996
                     /gene="ltrA"
                     /locus_tag="NK788_000383"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCH6768762.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            8113..8913
                     /locus_tag="NK788_000384"
                     /pseudo
     CDS             8113..8913
                     /locus_tag="NK788_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            8910..11441
                     /locus_tag="NK788_000385"
     CDS             8910..11441
                     /locus_tag="NK788_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768763.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            11491..11823
                     /locus_tag="NK788_000386"
     CDS             11491..11823
                     /locus_tag="NK788_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768764.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11824..12417
                     /locus_tag="NK788_000387"
     CDS             11824..12417
                     /locus_tag="NK788_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768765.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12580..14274
                     /locus_tag="NK788_000388"
     CDS             12580..14274
                     /locus_tag="NK788_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCH6768766.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            14962..16839
                     /gene="ltrA"
                     /locus_tag="NK788_000389"
     CDS             14962..16839
                     /gene="ltrA"
                     /locus_tag="NK788_000389"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCH6768767.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            16970..17347
                     /locus_tag="NK788_000390"
                     /pseudo
     CDS             16970..17347
                     /locus_tag="NK788_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            17347..18552
                     /locus_tag="NK788_000391"
     CDS             17347..18552
                     /locus_tag="NK788_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCH6768768.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            18568..19212
                     /locus_tag="NK788_000392"
     CDS             18568..19212
                     /locus_tag="NK788_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768769.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            19223..20029
                     /locus_tag="NK788_000393"
     CDS             19223..20029
                     /locus_tag="NK788_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768770.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            20051..20281
                     /locus_tag="NK788_000394"
     CDS             20051..20281
                     /locus_tag="NK788_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768771.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            20827..21102
                     /locus_tag="NK788_000395"
     CDS             20827..21102
                     /locus_tag="NK788_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768772.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            21144..21566
                     /locus_tag="NK788_000396"
     CDS             21144..21566
                     /locus_tag="NK788_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768773.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            21585..23177
                     /locus_tag="NK788_000397"
     CDS             21585..23177
                     /locus_tag="NK788_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768774.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            23189..23524
                     /locus_tag="NK788_000398"
     CDS             23189..23524
                     /locus_tag="NK788_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768775.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            23671..23865
                     /locus_tag="NK788_000399"
     CDS             23671..23865
                     /locus_tag="NK788_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768776.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            23897..26269
                     /locus_tag="NK788_000400"
     CDS             23897..26269
                     /locus_tag="NK788_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768777.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         9980 a         4136 c         5453 g         6845 t
ORIGIN      
        1 ttttatttgt tagttgaagt attaatatca acgatttctt cagccaattt atttaatttc
       61 atagcattta tgaatctcat gttgtctata ctagagcgtt tgttggcaag gtcacttact
      121 gtagttaagg gtacgccaga ttctatggat atagaacgaa ctgatttacc actatcaaac
      181 agcaaccatt cgattttctt tttatcagcg tacatttcaa tgctccttct tgttttttct
      241 ttattatagc tcttttttgt aatgaatcac aatatcaaga gaaaaatttt ctatagaaag
      301 gtggattttt atgagtattc gttctccgtg tatccaaatg aggtttgttg ctactttcgt
      361 tatttgatat actaaaaata aacaacgaaa gaaggaataa aatgaagaaa aagcaactat
      421 ggtttgtctc tatgtttgca atatttttta ttattttaag tggatgttca agtaataaga
      481 aatcggaagc taaagaagat aatagtttat tgcaaccagg aactgtttgg aaagaagaag
      541 taggtggtct tattcataat ttaaaaatta tagatgatac tacctgggaa tattctgaaa
      601 gtgtatggca tccagatcct gtacaaataa cagttaagag gcaaaaagac tatgaaggtt
      661 tagaaagata caaaatagta gattctgcag gcattaggga atttataaac aaacctgatt
      721 cattatttat tgttgttccc tatgagaagg atggcgtaaa aaaaataatt tttcttggat
      781 ctagcaagga tgagaagcga acgaaagaga aattaattca tgatggatca caatcaaata
      841 aatacaaatt acaaaagact agcgagtgat tgctagtctt tttatttgtc ctgaaggagg
      901 aatcacagtg gcaaaagcta aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg
      961 ccgcggtgta tctgaaacac agtggaaaca agaagtcctt gaaaaagcac aatttgacct
     1021 attacgggga aaagacaagg acttagaagg atatatctat caacgtgaac gtgaaaagct
     1081 tgttttcgat gaaattatta aacactaaaa ggagcgaaca attttatgtt tgaatcaatt
     1141 acaatgaatt ttttaacaca accgaaagta gtagctgcgg ttgatatttt tagagcatta
     1201 caaactgccg caactagttt gaaaaataat ctgatgattt ttgctgctgt tgcggcagtg
     1261 gcttccattg ctcttggagg tattttcttc ttctttgggc gaagaggagc tgaaaacggg
     1321 aagacacacg taggttatgt attgttgggg gtaggagtta ttttagctgc cgcttctatt
     1381 gtagtttcaa tgttctcaat gtttggacaa accccacaag gactataaaa aaacaagtag
     1441 aaggagtaaa aagtacgtgg ctacttttta ctcctttttt tatggaagga gaatattcat
     1501 gttttttacg atttcaggca atgcaaaatt ggataaagag ttaataagaa aagcaacgaa
     1561 gaaagtggga aagcgtactt cctttttacc gaaaacaatt aaatattctg tcattgaaac
     1621 ggaagaacag ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa
     1681 attgactctc acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt
     1741 ttttggctca ctggattatc aaatttcaga aggtttttat cctacattga ttgatttaat
     1801 ccgcctagat atatttaaag atacggatga aaccatgagt gtggaagagt cttatcgaaa
     1861 cgaaaaaatt ttacaagttt tagaagaaga gttagaaagc gaccaaagaa aagtgaatca
     1921 agtgagtgaa gtcgaagcaa acgaagaagt accatgggaa cgcccaatct ttccaaagga
     1981 tgaatcaaaa gaaaaagaga aaggaattat tgacgaacaa gctgacattt tgactcataa
     2041 atcaatattt gaggaagaga acgaggtaat gggtcaattg gaggtagatc cctttgaaga
     2101 aacaatggag gatgaagaga cggccgaagt agaagttgat ccagttgaag ctaatgaaga
     2161 cccaacatgg actgaagggg attctcagga atatattgaa ccacttatca gtgaagaagt
     2221 agagaaaaat gaaacagaga tacaaaaggc aacggtattt gattatattt tggatcaata
     2281 tccgccagag catgaatgga tgaaagaaaa aaataaatta tttttaaatg aactttattc
     2341 gaaatatcaa ttacctcaaa cctatcaagc atttttagag agtaaagaag aattaatctc
     2401 acagggtaaa ggcacacttt tagaacagtt agagtctgtc caatcaacaa attggcatga
     2461 gcttgcacaa gaagatttat tagctacttt tgaagaacgt caacaagaaa cagatcaaga
     2521 aattaatgag tatgcgacgg aacagtcgtc gaattgggag aaagcaaaag aggaactaaa
     2581 taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa attaaagagc ggtacgaaaa
     2641 gaaacgtgac aatgcgtatt ccttgtgtgt agaaaagatc aacaatttta ctcagacgaa
     2701 tcaagaagca ttatcgaaag aaaaagaaaa cttattaaaa gaacgtataa aagaacgaaa
     2761 agaagaattt taccgagggt tgcgtactga taagctaagg attagcaaag aattaaatga
     2821 acaactgaat aaactctatc aaactacgac tgaagttctg atggaaaaac acgagcagat
     2881 ccaacaagaa ttacaagcac aaattccaaa atggaaaaaa gagtatgaaa ttcaccaaca
     2941 agaattgaaa gaacaagaag aaaaacaacg gaaaaaagaa aaagagcgcg aacaattaga
     3001 actaaaaaaa cgtgaacagg atcttgcgga acaaaaaatg gcgatagaaa aacaacggct
     3061 agaacaagaa aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac
     3121 attgcgacaa gcacagttaa attatctgac acatcctcaa ccagtagctg ctgttcaaac
     3181 gccagtgaac aaccaagcaa atttaactcc ttcaccccag cgttccttta acggatggat
     3241 ggtgggatgt attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa
     3301 tcatatcaat caatcttcac aggctgattc tgtagccaca acggcgtcaa tcaaggaaga
     3361 agcccaggaa caagcaagaa aagagtatga acaacaatta gaagaattat tgaatcgtac
     3421 caatcagtca acaacagagt caagcatgga aaatagcaaa gagagtgatt ctagtaaggc
     3481 taaagaagga tcaagcgatt caactgtaca atcaaacact tccgaaagtt cagaatcaaa
     3541 tgaggtgaaa taatgcgtgt tagagaaaca acgaagcgaa aagacaaacg attcaattta
     3601 gaaaagaata ggtatctttc tcatttctat cgctatactc cctcaggaga agagctacca
     3661 gaaaaacatg acaaacgcat ttcgaaaaag attttttata gtggacttgt atacctccct
     3721 ttagagtatt tagtaacaag agtaccagaa gttttcaacc gattggaaat tgttaaggga
     3781 agcttacaag gaataaatct gtttcaatta caggaaatgc cacaggtctt tcattttgat
     3841 tcagctggtt tttggaatcc atttactcct acaactagtt tgggtacacg tttagcaatt
     3901 tcgacagttt gtgtggttcc agctgtactt atgggattga aaatggacat gtggtttcgt
     3961 cctctagcag atggacaaaa aggggataat cgattaatga ccttaaaaga aatccaacag
     4021 atttatccgc aaatacctga aacaaaaact acttttccag gttatggagg tattccaatc
     4081 acacattaca aaaaatattg gtacattcaa acagatactg ttaatacgtg tattgttggt
     4141 acctctcgat cagggaaagg tcagattgaa gtgttagcca ccattgacaa tttatctcgt
     4201 gcagaaaaac aaagttcaat ggtcgttaat gatccaaaaa cagaattata tgttggtagt
     4261 aaagatgaac tagaagctag agggtatgat gtatatgcct ttaatatcac cgatccactg
     4321 caatcaatga gtgataatcc attggcactc atcgtgaaat actggaaacg aggagacata
     4381 gatactgcaa cacagttaac caatactttc acaaatacga tttattatga tgccaacgct
     4441 tcagataaca aatactttaa cgaaaacgcg cagaaagctg tcaatgcgct tatttttgcg
     4501 ttgttagaac aagctgatca aacaggagat tactccaaat tgacaccgaa taatctcgtt
     4561 gagcttcttt ctgagattgg cggttttaat tattatccag atccagacaa tgaattcaaa
     4621 cagttgaatg ccttagatga atttatgaat cagttgccac ctggaaacat tgcaaaaaaa
     4681 caatatgggg caacgaaaat tggatctgat aaagcgaagg gaaatatttt atcaacggca
     4741 atcacgggat tgaatccatt tactctaacg aaaattgcaa agatgacttc tcaatcaacg
     4801 attgattata aatcggttgg gtttccaaaa tacttagatt tgaaattaca tgaaagtctt
     4861 ttaaatcaac gagtcgtagt agaattttat cataagggaa agaaatttca tgaagaacgt
     4921 gtcaaagtag gctataaagg atttagtgaa atcaattttg attgtcattt agttgcaggt
     4981 gatcaagttt gtttaaaata cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt
     5041 tctcaaagag tattgaaaga cgaagagggt aatatcgttt atcggaaaaa acgaggagaa
     5101 acccatttgc cagaattaga aaaagaagcc gttctaacat tagatgagga aaaaagtaac
     5161 ttattagtag agtctattcg gatgcactat accgataaac caatggctat ttttatgctt
     5221 acacctgatt atgatccatc taaccatatc attgttagta tttttcttag tcagttatat
     5281 aaagaattat ctacgcaatg tgttaagact aaaggagata aatgtcaccg caggattcat
     5341 tttattttag atgaatttgg gaatagtgtg acctgtttag cctaaggata aaagaggcta
     5401 gttccaacga acattggaat aactttacta tcgagaaatc aaatgacggg gtaacgccct
     5461 gaagggtttc cactgatact gcgacatgcg ttagtaggat gtgcatccaa acgcgtatga
     5521 agctcgctga agtcggcaga agggtaccgt aaggaaagac acgatacgta tctaccacgc
     5581 aagtgctcta gaggtagggt atgtacctga tatgtcgggt gtcctaaaaa gttcaataag
     5641 gtgagaatga tttgattatc tgacaaactt gcgaatggaa tgtctgaaac gaataactca
     5701 tagaaatgtg agggggaagg gggatacgtc ctctttccat tgactcgagg ttgagtaaaa
     5761 atgtgtttct atgaaaaacc ttgcatggtt aaaggctatg gagtagtcaa caggcaaaag
     5821 caagcaccta agttgaaatc tatggataga tagtaaggaa caggggaagc atctagcacg
     5881 gagggagcat accgaagatg tgtttctaag gaaaaaccga aatcggttat aacttaggtg
     5941 aatagatgtg aaagtagagc cacgaccagt gaaaatttcg taatgaaatt ggaggaacag
     6001 gctcaagtcg ttatttataa tataagaaac ctatttaaaa tcatggattc tcgtaagact
     6061 aaaaaggtca cgaaccagaa tggagcgtga taaaaccatg actaacacaa aaggaattag
     6121 cttaaagaac aaacaactca gacacgccga atactacgac atgactgaga ctttcgatga
     6181 attgcacgca atgagtgttg caaaatcaaa ttttaaaaat ctcatgaaat tcattattga
     6241 tgacaataat atctttctag cgtatagaaa tatcaaaaag aataaaggaa gtgcaacggc
     6301 cggatacgat ggtgtcacta taaaagacat tgaaaaattg gcatgtggtg actttttaac
     6361 tattattaaa aggcgattta aggattacag accaagaaaa gttaaaagag tagagatccc
     6421 gaaaccaaac ggaaaacttc gacctttagg aattccttca atatgggaca ggatagtcca
     6481 gcaatgtatt ctacaaattt tagaaccaat atgcgaagca aaatttaata agcatagcta
     6541 tggattcaga ccaaattgtt ctacagaaaa cgccatagca gattgtcttg ctagaatcaa
     6601 tcaaagtaag atgaattatg ttgtagatat tgatataaaa ggattctttg acgaagtaaa
     6661 ccataccaaa cttatgcgtc aaatatggac aatggggatt cgagataaac aactccttgt
     6721 aattattcgt aagatgttaa aggctccgat tgttatgcga aatggcaatg ttgtttttcc
     6781 aaccaagggt actccacaag gagggatttt aagtccactg cttgcgaata tatgcctgaa
     6841 tgaatttgat tggtggatag ctaatcaatg ggaggaacga actgtcaaag agctgaaaca
     6901 ccactatcga aaaaacggag catgggataa aggaaatatc tattcgaaat taagaagcca
     6961 cacaagttta aaagagatgt atctggtacg ctatgcagat gattttaaaa ttttctgccg
     7021 aacaggagaa gaagcccaaa gggcttttta tgccacacaa aaatggctca gagaaagatt
     7081 aaagctccca atttcaatgg agaaatctaa agttacgaac ctaaataaag ctgaaagtga
     7141 attcttgggt ttttcaataa gactccacaa aaagggaaaa aagaaagtgg ctttcacgta
     7201 cgtttgtcgc aaagcagaag aaaacattaa aaataaacta aaacaacaga ttaaaaaaat
     7261 tcaatacgct ggagcaaaaa aaaataggca ccgagcaata ggtgactaca acatgatggt
     7321 catcggtatc cacaattact atcggatagc ttcacattgt agtgtcaatt tcactgagat
     7381 ttatagaagt gttaggacag taatagaagc aagactgaaa gctagaggaa tgacgaaaaa
     7441 tggaatctat ggacaagatc atgaaggata ccgtccgtat ctaaagtcta agcaaatgag
     7501 atatattgaa ggttttccga tacttccaat tggctatatt aaaaaaagaa ctccactcaa
     7561 caagcggaga aatctaaata aatataccaa ggaagggaga aagctcattc atcaagagca
     7621 acaagctata gaaagctgga aaatcaagta tcttgtaaaa ggttttagta acaataagaa
     7681 atacacggtg gaattttctg ataacaaaat atctaagttt atttcccaaa aagggaaatg
     7741 cgctatcaca ggaaacgaac ttttgatgga agaaattgaa tgccatcaca ttaacccata
     7801 tcatttgtcc aaagacgata gttacaagaa ccttgtcatt atctctagag aaatccatag
     7861 attaatccat gcaactagtg aagaaactat tctgaaactg cttcggttac tgaaactaaa
     7921 tcactatcaa attagaaaac taaacacgta tcgaaaaaaa gcagctaatt ttccaatatc
     7981 aatcaaaatg aattagatag gaaacaaatt atattgtaat taacgatgga acgccgtgtg
     8041 cggtgaaagt cgcacgcacg gtgtggaccg ggggaaaaga cggagataat atcaaactct
     8101 tacctatcgg aattgccacc aatggataat atggaaggaa tcatgacggt cacagctggc
     8161 cgaaacatgt tgtgggatct gtttattcaa tcttatcagc aactttatgc gaagtatgga
     8221 gaacaaaacg cagcgattat caaagaaaat tgtcagacac atatttatat tatgtcgaca
     8281 gatgataata ccatcgaaga attttctaaa aaagtgggga ataagacggt tgaacaagag
     8341 aacagtacca tcaattcatt aggtatgaat actcatatca atcgcaatgt tgattcagat
     8401 cgtatcttaa ctcctgaaag aatttctact tttttagaag gtgaaacgat tgtactcgct
     8461 cctttaaaac gacaagattt gaaaggtcat aaaatacggc cgtatcctat ttttaatcat
     8521 ggggaaacga atcttcctta tgcttatcaa tatattgaag atttcaatcc atcgtgggat
     8581 atcaatgata tagacataca ttgtacacat gcggacttgg atcttaaggc tttacaaatt
     8641 gattatgatt atttcctacc agatgaatat gctagaaatc tctatcatgg actgcatgat
     8701 caattagaag aaaaaaacga agaaaatatg ttttataaaa tcgttcaatc tcttgagaat
     8761 gagacggcta aaaaattgtt aattcaattt tacgaagaac aagcagcaga tcgacttcaa
     8821 gaatatgttg aaaagttgat caaaaatgaa gaaatttctg aaatgattgg gaaacggttg
     8881 ttattggaat ctgaaaaggt ggtgaaagaa tgatcattgg aggaattttt tctggcatag
     8941 ccgactggtg gtcaggctta ttcggagaag gggatgcaaa ccccaaaacc gtaacgaaag
     9001 tcttacaaga ttatggtaat tatctgcagt atcaagatgt gttcagttgg atttgtaata
     9061 cttttttatg ggggttgatc aaagggttat ataatctcaa tactttacta gaaaaaacca
     9121 tttatcaaag ctttgattta aaagacttat tgaacgccgc tggggtaaat gaactatttc
     9181 aaagtttgat tagtaaggta ttggctcttt tttgtgtcgt cacattaatt tatttaggct
     9241 tgaaattttt tatgagtaaa cacccaccaa aagtcaaaaa tatcttggtt aatttattta
     9301 tggccatgat cttgattttt ggtggtagtt ctttaatcga tgaagggtta aatatcagta
     9361 aaagctttta cggagacgtc actgcagcaa ataaagcaga taaaacgggt tcacctgctt
     9421 ttcaaattat tcaaaataat gtgatcgatg tcagtacatt actagcaact gacccgaata
     9481 aagtagaatc cctgccaaca gacaaaagaa atgctttaac agcgaaaaat tttaaaacag
     9541 cgaatatcaa tgggattatt actcctaaac aagctaaaga aatggcggat aaagataaga
     9601 gtggtttatc agatttacaa aaggatcggt taagagcgtt acaataccga ctggaactgg
     9661 atgataatgg aaaagaaatt cctgtgcaaa tcagtgatga aggcttagcc aaatatgttt
     9721 atacttcagg gtatcgtcga tacattagta gtccagggat tattttagct ggtgaatctt
     9781 ctttggcggt ggcctattta tttatcctat ttacgatcgt tacttgcatt attgagctgg
     9841 tatttaaaaa attctattta gtgattgccg cgtcaacgga ctttgagaca ggacaacgga
     9901 tgaaaagtgc tatcgaagat atttctcaat ccttcttgtt attggcattt acaattttag
     9961 aattacgaat ctatacctta atgctttcag gtattggtga tttgcatgcg gcaggaaaga
    10021 tcaatggatt cttgtatgtt gtcgctttga ttgtactaac cattgcgcta ttcaaaggct
    10081 ctcaatcagt tacgaaaatt ttcggcgttg atacgagtct gaaaaacggc agtaattctc
    10141 ttcttagcat gtttgcctta ggaaatattg cgaaaaatgt gggtagcgga gctaaaaacc
    10201 ttgcaagtgc tggtaagagt ggtttaagcc atttaaatga tattcgtaaa aatggatttg
    10261 gccatcgaaa agatgttacc cctgataacg gggaagaaca atcagacgat aaagctacaa
    10321 ctgtacctaa tagtaagaaa acagtttccg aaatgttcgg gaaagcgaaa aatggtgttt
    10381 ctaaagccgc agaaggtctt ggttacgtca atgaacgtgg gttaaaagga acagctgaag
    10441 atcttacaga aaaagccaaa gacaccttag aagataaatt agacggcacg agagctaaag
    10501 aattggctga agctgtgaag aatccatccg aagcactaga aaaacaaaaa gaaaaagctt
    10561 cagaagtcaa agaaggacta aaagatgcgt atggtgaagg acaagtcaat gcagcagtga
    10621 aagctaatac aacaaccgaa aaagaaaatc aagaaggtat agtgattcct tcagtcaaag
    10681 atcaccaaga agcgttgcac tctaatgaaa attcgattga agagaaaaat tccatagaag
    10741 aaagaaattc agaagaagga aaaaatggac agttaccaaa aactgatcca gaacaatatc
    10801 gctataaacc taacagtgac cttgaaaatg agaagcgaat ttcagattta tcaaaagtaa
    10861 atcaagaaga caaagcagaa aatgagggac agttaccaaa aactgatcca gaacaatatc
    10921 ggtatagatc tgttaactcc gaaaatgaaa aacgcatttc agatttacca aaacgaaatc
    10981 aagaagtaca aacggatgag gattccatcg ttctgccaaa tgataatgga atcgaagttg
    11041 gaactgaaaa atcaacgaaa attggtttac ctaaggcaca aaatttacca atgaaagata
    11101 gtcgtttgaa tcaattcaag gataatgatc aaagttttgt tcatcaatca cctgcaggta
    11161 ctgttcaaat gaaaccaagt cgtttgcctg aaaatggaaa tctgacaact gtttcttaca
    11221 atttatctga aagtggtgag acagggaatg cgtccgttca aaatgttgtg acaagagaag
    11281 ctgcaccaaa acctattcaa aacgttcata caggttacaa gccagcaaat gtggcattta
    11341 ctgcaccaac aaaaataaat gtgacaagag aaactaaaaa caggaacaat gcattgaaag
    11401 aacaactatt gagggatatc aaacaggata tcaaaccata aaaggcctac aagttaggcc
    11461 ttttctttat atgaaaagga gaaatcaatt atggataaat atataaacat gaaaaaccct
    11521 ccaagattta gtggagaatg gaagtttttc gcttttttct ttttagatct attaacgctt
    11581 ggtggctgct ttttttgggc gaaaatggtc aataatcttg tccacttacc actctttttt
    11641 gcactcttga attatggctt atggattgcg atagcgttag tgattgtttg gcgaccctat
    11701 aataacccag gtcaacgaca aatttttgcg attatcgagt ctattttttt tgcggacaag
    11761 aaccattatc atgcgttaga tcctaaccga aattatcatg gtacgtataa ggaggatgaa
    11821 taaatgcgag tcaaaggaca aaattcagtt gaaaggaaat ccattttgca gtttggaaaa
    11881 ccagaagagc gctctatctt ggacttattt ccgtataaat cctgtacaga agagggagtc
    11941 ttaattacta gagaagatca ttttcaacgt tattatcgtg tagtttctac agacgtggaa
    12001 ggattaaatg aacaagagaa actagaacga atgaatcagt tgaccaccgt catgcgtacg
    12061 tatgtgccaa caattaagtt agttagtttg accacagaga cggatctaac ggaacaaatc
    12121 gtgcaaaagc ggcagttatt agataaaaat cgtatggaac agtccttagg aagaaatcta
    12181 cagcgattaa gaaaatatga aaagaaatta gtagaggaaa tccaggaatt gaaacgagca
    12241 gaacaggaac gccctgattt aagcttcttt ttcgtcattg aaggaaagac aatcaaggat
    12301 ttgcaaacca gaaaccgtca gttaatgcga tctagtggca ttttagggtt aaaaccactc
    12361 tacaaaaaag aattgatcaa gattctttat cgaatgaata atatgaatga tgagtaggaa
    12421 tggggaagag agaatgaaat atccgttgat atggttagaa aaagatgttc cgaaagaaga
    12481 gaccactaga aaatttcctc acactttaag cctgggtaag ttgggaaaag gaaaatcttt
    12541 taaccaagta aagaatcaaa caagaagagg tgactgtaca tgttagaagc aattaaaaca
    12601 gctttttcac cacaaaatga tcaaaaggta cttgatccat taaagcaact aggaaatcgg
    12661 gaaatagaaa ttttaaaatc caaagggtat gatttagact ttttaaaaca agtgatgcca
    12721 caaggtggat taaatttcga tgaagagtat gtgacctatg gaaatggtta ttcacgatgt
    12781 gtgacagttt atcactacac aaaaaatcct acgctttttt ggttggccaa tctcatgaac
    12841 aatcctcata cagtggcaac actagatgtt agcacagatg aaaagcaaaa agtgattgat
    12901 caaatcaatc gtgccttaga tgaattaaat gatcggtcag ataatgaacg aaaagcgact
    12961 gaccgaaata aaagcgcgga agaataccta gaactagcgg aatatgcccg aaaattgact
    13021 tctaatgggg aaatttctaa acaagtcaaa gtgagagtac atgtttacgg aaacacgatt
    13081 caagaagtag atgaacgagc acaggaactg atcgaaaatc tcaaagggat ggattatcgt
    13141 gcagtgacct atctgcacaa aacgaaaaga gactggcaat cgatgttcac catgttcgat
    13201 gaacaagctg aatggtttgg aaatatcaaa gggcaatcac taccatcgct aaacattggc
    13261 ggaggtttcc cgttccacca ccaaacgcta aaagatccag gtggaacaca atatggggaa
    13321 accatgacag gcggcccgtt tgtttggaat gccacgtata aaaatagtgt gcggttgtct
    13381 accaatacct tgcttctagg aatgatggga gctggtaaat cgaccgttct taaaatgatc
    13441 gcggaagccc atttagctgc aggagacttt atttggggct ttgaaaaggg aaaagacttt
    13501 attccctttt taaaggaata taacggcatt atggtgcgtt tagacggatc tgacgggatg
    13561 atcaatcctt tagaaatatt tgccacacgt acgtatgatg aagcttccag tttatatgac
    13621 gatggatcag tcaaagattt gaagatcaat gaagcagcgt cctatcaaac gcacttagac
    13681 aaagtggtct atcaggtaca gttagtgagt cctcagctaa aaggaacgat gaaagcagaa
    13741 tttaagacct atttaaatcg tttttatgaa gagtatggaa cagtaccacg cggctttact
    13801 tcctcaaatt cgcgttcaaa tactgatacg caagtcacag gtaaagatcc agaagcttat
    13861 ccgacgttca aagaattttt agacttttta ggacaactgg aattaccagg tgcttcccaa
    13921 gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag aatccttatg tgagacgtat
    13981 ggcatgattt ttgatggtca tagtacgatt cgacaccttg accaacaaca gttggtcttt
    14041 tttgacattg actctattac aggcttagat cgaggggtac aacaatgtca aatgtacatg
    14101 gctatgacgt tgatttggaa tcaggcattg attcaaggaa gaagacaacg acgtttaatt
    14161 aaagaaaaac aaatggaaga acaagacttc aaatgcttct tggcgatgat tgatgagtgc
    14221 cataatggtg cgacctgttc tgcagtgaaa agctgtgggt tgcaagtagt ctaaaaacta
    14281 ttgtaataac tcttataacg agaagtcaaa tgagtgggta acgccacaaa gggcttccac
    14341 tgatactccg acatgcaaca agtagtttat gcagaccaac gttgtgtgaa gctcggtgaa
    14401 gtcggctgaa ggataccgta aggtaaaaga aatagtgaat accacgaaag ttctctagag
    14461 gtaaagggtg tatccgataa gtcgggtgtc caaaaaaact taatatggtg agtatgtaca
    14521 aacgtactga cgaactagcg aatgtactgt ccgaaaggca tagctcctag aaatgggaga
    14581 gacagtgagc aaaaaatcac tcattgtgct aacttgtggt tgagtaaaag tcgttgttat
    14641 gaaagaccat gctgcgttac aggcgtagga gaagttaggg ggcaaaagct agcatctaag
    14701 ttaaggcatg tggatagata taagggaaca ggggaagcca tcaacatgga gagtgcatac
    14761 tcgatgaagt ggtagtaaga aatatgtgaa tgacaaataa cttactttag tgatggtgaa
    14821 agtagagtca cgactgatga aggttctgta atggaactgg aggaacaggc tcgattcgtc
    14881 tgtaaaagtc atgaaacccg ttcaaccatg ggattctcgt atgactaaaa aggtcacgag
    14941 ccaattggag cgtgataaac catgacaaaa gaaacaagtc caaaagatag gaagttgcgg
    15001 catgcagaat attatggcat gacggaaatc ttcgataaga tatactccaa aagtttgaag
    15061 ggagataatt tcaagaatct aatgcagatt atcatttctg ataacaacat tgtcctagca
    15121 tatcgtaata tcaagagaaa tcacggtagt gtgacaagag gagaagatgg agtaacgatt
    15181 aaagatattg aaaatctcaa ccgtaatgaa ttcgtagaaa ttgttaggaa gcgattcgca
    15241 cattataacc ctcggaaagt aaggcgtgta gaaattccta aatataatgg taaattaaga
    15301 ccgttgggta ttccatcaat atgggataga atcgcgcagc aatgcatctt acaagtatta
    15361 gaaccaatat gtgaagcaaa attcaataat cacagctatg gtttccgccc aaatagatca
    15421 gcagaacacg caatagcaga ttgtatggcc cgaatgaatc aaagtcacat ggaatatgtg
    15481 gtagatgtag atatcaaagg tttcttcgac gaagtcaatc atactaaact catgagacaa
    15541 atctggtcta tgggtatacg agacaaacag cttctcttaa tcattaggaa aatgttaaga
    15601 gcacccgtag tacttccaaa tgggaagttg atatacccaa caaaaggaac accacaaggc
    15661 ggaatactta gcccattact tgcgaacatc aatctcaacg aatttgactg gtggatagca
    15721 aatcagtggg aggaaaaaga tgccaaagag ctaaaacatc attttaggaa aaatggagct
    15781 tgggataaag gtaatatcta tcacaagctc agaaagagta caactctaaa agaaatatac
    15841 ctagtgagat acgcagatga ttttaaaatt ttctgtagaa attataatga agcgaaaaaa
    15901 gttttctatg caactaaaat gtggttacag gagcgtctaa agttaccaat atctgaagaa
    15961 aagtcaaaaa tcacaaatct ttcaaaagaa gaaagtgaat ttttaggctt tacgatgaaa
    16021 ttagtgacca aaggtaacaa aaaggttgtt aatacgcata taggtacaaa agcacaaagc
    16081 atgattaaag tgaaactaaa aaaacaaatc aaacgaattc aaaaaagtcc taatagcaaa
    16141 gataccttta tctatattgg agtctataac agtatggtta ttgggattca taattacttc
    16201 aaaattgcga ctcattgtag tgtagcaatg cgaaaaattg caagagaact cagaccaagt
    16261 tttgaaatac gactacgaga acaaggaatc tcaaaagaag gacaatacgg aaagtacgaa
    16321 ggatacaagc cttacctacg gtcaggacaa atacgatata taagagggtt tccaatatta
    16381 ccagtagggt atattcaaca cagacatccg atgtgtaaaa aaagaaatat taataaatac
    16441 accgaagaag gtagggaagc cattcataag aagcaacaaa ccgtagaaga atggaagata
    16501 tcgtggttgt gtagcaatcc agtgcttagt aagagagcaa cgattgaatt tgcggacaat
    16561 cgaatttcaa aattcatcgc tcaaaaggga agatgtgcag taacaggtga ggagcttatc
    16621 ttatctgaaa tgcattgtca tcatattatc ccttatcatg aaagcaaaag tgatagctat
    16681 gaaaacctag tgattgttac tgaggaggtt catcgggtaa tacacgctac acaaagtgaa
    16741 acgattgagg aacttctaaa atatctaaaa ttaaatccaa aacaaaaaga aaaactcaat
    16801 gagttgagat taaaagttgg taacgaagaa atatcttaat aaatatttgt aggacgggaa
    16861 aacagaatgt cttttataga cgagggaacg ccgtatgcgg tgaaagtcgc acgtacggtg
    16921 tggactgggg gaaaagacgg agataatatc aaactcttac ctatcagtat cgattaatcc
    16981 agattttatt gaaacggtaa attatgtcac aaactttcaa cgagaaatgc gtaaagtaga
    17041 agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa atatgtcttc
    17101 tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga tctttatgcg
    17161 tatggatgaa tctattttac gtcatataga aggattagtt ggtaaatcaa tgacaaactc
    17221 agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt ttggctcaaa
    17281 agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg
    17341 aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt atgttaagcg tcgtgcaaag
    17401 cgacgcttat ttttgtggtt gtttggtact tctgcaggtt tgattactat tttgattact
    17461 gtgttcgtga ccctttttct aatacttgcg gcgggttcga ttgataattc tgattcagat
    17521 tcaagtagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc gatttataaa
    17581 gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgctgt aggtgcagca
    17641 gtgaaatata aattattacc aagtgtgatc ctttcgcaat acggttatga atcagcgttt
    17701 ggtacgtcgg cgtccgcaag aaatgatctc aactattttg ggattacttg gtttgatggt
    17761 tgtctgtttc ctaaaggaac tgcgcgagga atcggtggta ttgaaggtgg ctggtacatg
    17821 aagtttccca actcaaaagc agccttctcc tattacggct ttatggtagc cacacaatcc
    17881 aattttaatg catgtgtagg aaataaaagc cctggcgcaa gcttattgat cttaggacga
    17941 ggtggttatg ccgctgcagg aattactgaa gatagtccgt attatacagg ttgtatgtcc
    18001 attatcactt ctaataaatt aaccgaatat gatgaattcg ccatcaaaca ttggggtgaa
    18061 ggtggcaata acaacggcac gatcactggt gaatggacga atccattccc tggcagcagt
    18121 ttagataaaa gttccttcag tggtggtcaa ttatttggaa caaatccagg cggtgaattc
    18181 cgtccgaatg gcttccatga tggattagac tttggttcag tcgatcatcc aggaagtgaa
    18241 attcacgcgg tgcatggagg aaaagtcgtg tacgttggca atcctggtat ttcaggttta
    18301 ggtgcatgtg tgatcgtgat taattacgac ggtttgaata tggtatatca agaatttgcg
    18361 aatagtacag gaaattctcg tgtgaaggtt ggcgatcaag tgaaagttgg tcaagtaatc
    18421 gggatacgag atacagcaca cttgcattta ggtttcactc gaatggattg gcgtcaagca
    18481 caaggacacg cattcacaga cgatggtaca tggattgatc cattaccatc tttgaatagc
    18541 agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa ctttaaaaat cgtcttaaca
    18601 gctgcaatta gcgttagttt aatcggcaat atcgcgttgt tttggcagct gaaagaagaa
    18661 caagagacaa atacctttaa aattgaagaa gtggctaaaa acgcgtctga gaagctcaaa
    18721 aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa atcaaaccat caatgagaaa
    18781 aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact
    18841 gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa atcattaagt
    18901 agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat cgacaaatta
    18961 agtcctgcag acgatgatca ccaacatgat gattacggaa gttactcctt tacattaggt
    19021 gacgtgcaaa cttatgctca aacaagcagt gtaaaagagg aacaaaattt tgtcgtattt
    19081 gttgattata caatcggcaa tccgcaattc aaagatgtca aaccacaaaa aataaaaggt
    19141 ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcataga ctttagttat
    19201 ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gaaagacaac gtttcaaaaa
    19261 acgaaaaagt tatcttcaat ggctgggaat ctttatgatt ctcggctttt tgattcttgc
    19321 tagtccgatt tatacgaatc agaaggtaga tataccagat gaggtaagca cgaacgcctt
    19381 tttaaacacg aaagagctga caatggtttc aaaagaaaaa ggagcgaacc agcaattagt
    19441 ttttgaattt gcgatcgatg atgaagatca aagcttgtta aaagaactgg ctaatcttaa
    19501 ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa gcaatacgag ccaaagtgat
    19561 caaggtgtct gatgattatt ttgcagtgaa gctaacgcat gtaccagaag aatatatcgc
    19621 catgcgttta accattattc ctgaaaaaat tgatccaaaa gtggatatgc aagaaccaca
    19681 agatctgatt tattacgtgc acgaagataa ggttaaagat caagtgaaag atgaagatta
    19741 tgaacaacac gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa aagcagctca
    19801 aaaacaaatg gaatctttcc aagcaaccat tgatttgaat gaagaattag tgaagaagct
    19861 aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa aatactgaaa acaagattaa
    19921 tagctaccag caagaaattg aaacatcgaa aacgcaactg aaagatcaag aagagatcat
    19981 tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aagatttaga aggacaggtg
    20041 gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat actttcgctg agcagaaagt
    20101 attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcgag aagtgaattt
    20161 atctaacaca acaatatctg agttgaagaa tggtcgaaag aatttagata ctttaaaatt
    20221 cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg ataacaaata
    20281 aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa tcagctatat
    20341 caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct aacgcaaaca
    20401 tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa ccaaacagga
    20461 agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat atgacaaaat
    20521 atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca caaataacct
    20581 taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt gttaggtagg
    20641 ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat ttgtgtgtag
    20701 atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg tgtagcaaaa
    20761 tcaaaaaatg tgtagggata tgtgtagcta cacatatccc tacacataat aaatttggag
    20821 gaaaatatgg acttattaat tagaaatatt gaagaaaagt atattaataa aattgacaaa
    20881 cgttgtgaag aacttacggt caagactggc aaaaaatgga gtcgaaatca atacctaaaa
    20941 gttttgatcg aaaatgattt tgatcacgct cttctgaatt ataaaaaaga ccaatttgat
    21001 cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaacttt agaagaatat
    21061 attgcaacaa ataatcaatt aattggtttg ttaattgaat aataaaaata gtattgtgaa
    21121 tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta tatctttacg
    21181 atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata tctaattata
    21241 gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa aaaataaatc
    21301 caattcaaat taaattgaat gcaatgggaa aagaaatttc tattttaacc gaaatggttt
    21361 cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca aattcaactg
    21421 tttatcaaga agcagaagaa cgtgtggaag gaaaaattaa atccaatcaa accaaaaaat
    21481 attcttctaa gttaaagaaa ccagtgaatg aatctgttca taccagagta gatccatttg
    21541 atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat aaatcgcctg
    21601 cagttattct tacttgtcaa tttaccttgc ccaataaaaa atctttttca acatacattg
    21661 attatatgac aagagaaaaa gcactggaag aaatggaacg acgtacgccc gaagaagaac
    21721 aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa ggagaaactt
    21781 tttcggaaag taagacggat aaagaattca gtgaaacgtc gaatgaagct caatcgttga
    21841 tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa tatctttctt
    21901 atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagg gaacaaaaag
    21961 aataccaacg gttgaaccaa gcaatgaaaa aatatcaaac agagccacat aaaaaagctt
    22021 caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga agtgatctga
    22081 aggagatcaa acaaatcttc caaagaggac aacaaaaagg ctcaatcatt tatcaagatg
    22141 ttatctctca cgacaattcg tatcttgaaa aaatgggctt gtacgatccc aaaacagata
    22201 ccttagatga agaaggtttg aaagcagctg gaagaaaaat gatggagaca ttattcgaga
    22261 aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac actaagcata
    22321 tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccgtat actgagattg
    22381 aaaatggtgt gagttatatc gtaccgaagg ggaagagaag acaagcaaca ttagacgcaa
    22441 tgaaaaccaa ttatgtacat gagttagaac gtttctctta tgaaaaggat cgaatgatcg
    22501 gtatggagaa acgaaattta ttgacacgaa aaagtgattt gcgtaacacc ttaacaaaag
    22561 tagtaaaaga accagcacgt tacgatcgac acgccatgaa cttattaaat gaagtatata
    22621 aaaccctacc tgaaaaacgt tcggaatgga attatggaga tgaaaatcga accaagcttt
    22681 caccatttac aaggggaaaa cttgatgagt taaccaaata tgttttaaca aaagaacctg
    22741 gatatcaaga atatatacag atttcaaaag cattgaaaga agaagccaaa agcctttatg
    22801 gggaaagcaa aagagaaaca aagaatgcag aaaaaaacgc cctctttgat ttaaaaaagc
    22861 gcacaggaaa tgcgatttta acagaactga agagacagga tagtcaaata aaaccattaa
    22921 tagaaagtgt caatcagatg caatctattt acaatgaacg atcctttcga acactcaaac
    22981 agttgccaac aaatgaatac aagtatagtt atcaagaatt cgtaaagcgt aggaaacaat
    23041 tccaacaaag agttgtcacg cagcgaagct tgcgtcaatt aaatcgaaca attaatgcac
    23101 atgaagaaaa ataccacgca agtaaagcat atgaagagca acagcgaaga atccaaatgg
    23161 aacaagaaag acaataggag gaagagaaat gaaacaatat ttagatcaat ggaaagtcat
    23221 agaaggatca ctaaaagaag aacgaatcga acaacttcct gattgtttag aaaaagagca
    23281 tttgtttcag ataagcgaaa tgttgagaaa cgaacagttt gatccaaatc aatttttagt
    23341 tgttgagtac ccagctactg gtgtgtattg ttgtaatcat gtgaagggtg aaaaatattt
    23401 tattattcaa gaatacgaag gtaagttagc gccttattat actacttggg aaataaatga
    23461 agagggaatc aataatttcc catgtgaaag cattgaagag tctatttctc tcacggaatg
    23521 ttaaataaat agagacgtaa aagagtcctg aacgtgattg tccaggactc tttttcaatg
    23581 aattgttttc cgccaattca ctgagtggtg cgattcaatt ttattatatc aaaagaaagt
    23641 gagattccaa agtgaaggga ggaagaagaa atgactcaga caacgattgg tgttgtggtt
    23701 gaaaaaagca gggataatct cgtttttgtg acagaaattc aaacaggtag agcttttgta
    23761 gtcacagaca aagctgcaaa agcgtatcaa aatggcaata ttttaacgtt aaatctgact
    23821 acgaagatgt tcgttgatgc agtagaaaat tatccatttg tttagacaaa cgatatttta
    23881 caaggaggaa acggacatgg cagaagaaaa agaaccacta gataatagtc gtttgggaaa
    23941 gtccaaaaga aaattagtac ggttgcaaaa cgaattaaat aatcaatttc aaaagctatt
    24001 tgatcaccaa aaattgacaa atgggcaacc aatgaatgac aaacgaaatg gtcgttcatg
    24061 gtttaaccag caagaaaaaa tagaaaataa aatacaaagt ttgagagaag agatcaagca
    24121 acaagaaaaa caggtagaaa aacttgaaag acaagaagag ctaaaggaaa tgggttataa
    24181 caagtacggt ggcctggaca tgacgatcga aaatattcca agaatcaaag aagaaatcga
    24241 acgctttgaa aaaggtgaat cgactttttc agctgccacg atccgaaaat accaacgaaa
    24301 attggaaaca ttagaacagc tgaaagaaaa atcagagaaa ggaaaagaaa atcttcttcc
    24361 tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaga atccaactat
    24421 ctattttctg aaaggctatc gaaaagtcgc tttagaactc aatgaaaacg ggggatttga
    24481 ggaatctaaa aaataccaag ccacgtcgga agaagaaaag gcagctgttc gtgaattact
    24541 tgaagaagct acacctactg attctgtgaa ggagaaagaa accgatcatt tctatcgagt
    24601 agaattcaac gaaaatgatc cagcaaaagg gttgaaaagt tatggtgggg agatcgtaac
    24661 actagaattg atcgatcaac tcaaaacact ggataaccaa tacgccgcag aagcaggcta
    24721 ttataaattc tattttgaag aagtgaagaa tggagaagta gtcgatcaga tacgaatgga
    24781 tctaggggat ggcgtcaata gtaatcaccc catctattta gagttagcta gattgtgcga
    24841 acaatcacct gaaaaggaac aagaacaaac agtcaaaaat gaacctattt caaaagtaca
    24901 aaacgagatt aaagagacaa aaacaaaaca aaagggttat caaaaacgaa cggcagaaga
    24961 gattaagcaa gaagtcaaat cactttccca acaggcgctt gatgcggtga aaaagcatac
    25021 tcattcacct gaagacgtca aagaactttt gaattttatg tctcgttttc cagaacgatc
    25081 ctttagaaat caattattga tcgaggaaca atttccaggc gcaacggctt gtttaggtcg
    25141 tgccgcgttg aaaaaagaag gaatctatat caaaaaagga gaacaaggga acaagatctt
    25201 tgtcagaaaa acggtcaaag gattttatga gaaaggacgt ggctttgttc gagaaacaga
    25261 ggctacacca gaagataaaa aacgaatcga aagtggtcaa atcgaagtga tcaaaaaacc
    25321 ttactacacg attgaaaaag tgtttgatat cacacaaaca caattgaaac ctgaagacta
    25381 tccgaaaatc ttcccgaacc gcgtctttga ttttcaattg gatcaaacag ggcagacaga
    25441 acttcaagtt gggattcaag cggtagccaa aaatattgga attcaaattc gagatatgac
    25501 agaaagtgaa gtctatcaaa gagaattagg acaagcacgt ggggcgtacg ttcgtaacga
    25561 atttactggc gaagaagaaa tcgtcatgaa tactcgaaat actcctacgc agcatttagc
    25621 cacaagtatt cacgaattag cccatgctag aatgcacaaa ttttcagaat tagatactgc
    25681 aacaaaagaa ctacaggcag aaatgacctc ctatattgtg tgcaagcatt ttgggatgga
    25741 cacctcggag aaagctattc cttatattgc gacatggacg aagaatagtc agacgttgga
    25801 tgataaggaa gctgcggaac gtgggaaaat catgaatgat gtcagtcgtg tcgcaaatga
    25861 attcattcaa accatttcta atgaaatcaa tcaatacaga gaaagagaac cagagatcca
    25921 gccagcagaa gtgaagcagg agcataaaaa tactgtagaa acggcagaac aaaagaatat
    25981 tgtgcccgtt ggctcacttt ggatcgacaa gaaagacggg aaagtcctgg aagaagacac
    26041 gggccgaacg ttacatttga aggatgcggc gtttattgaa gatccgcagc ataatcaagt
    26101 gattttaaaa ggaaaaagtt atggaaaaga gatccaaacg gcgttacctt cagaagaatt
    26161 tgcgctagga aatccattcc gattttcact tgagacagtg tcagaagtgg caaaacgaaa
    26221 aaatgaaatc tattatgaac aacaattgtc acaaaaagaa cgcgtataaa acaagcaaga
    26281 aagactatct gaaaaatgga tagtcttttt tcttttggga ggtttgaaaa tgacagagaa
    26341 aaaaatgagt ctcattgatc gatgcaagca aatggatatt gtggattttg cacgaaacaa
    26401 tggtttagcg gtgg
//
LOCUS       DAHUWM010000014        25423 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHUWM010000014 DAHUWM010000000
VERSION     DAHUWM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.014"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            3..485
                     /locus_tag="NK788_000401"
     CDS             3..485
                     /locus_tag="NK788_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCH6768778.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            522..1532
                     /locus_tag="NK788_000402"
     CDS             522..1532
                     /locus_tag="NK788_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCH6768779.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1647..2360)
                     /locus_tag="NK788_000403"
     CDS             complement(1647..2360)
                     /locus_tag="NK788_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768780.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2550..3140)
                     /locus_tag="NK788_000404"
     CDS             complement(2550..3140)
                     /locus_tag="NK788_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCH6768781.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3624..3851
                     /locus_tag="NK788_000405"
     CDS             3624..3851
                     /locus_tag="NK788_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCH6768782.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3867..5033
                     /locus_tag="NK788_000406"
     CDS             3867..5033
                     /locus_tag="NK788_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCH6768783.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5267..6310
                     /gene="hrcA"
                     /locus_tag="NK788_000407"
     CDS             5267..6310
                     /gene="hrcA"
                     /locus_tag="NK788_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCH6768784.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6404..6967
                     /gene="grpE"
                     /locus_tag="NK788_000408"
     CDS             6404..6967
                     /gene="grpE"
                     /locus_tag="NK788_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCH6768785.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7018..8847
                     /gene="dnaK"
                     /locus_tag="NK788_000409"
     CDS             7018..8847
                     /gene="dnaK"
                     /locus_tag="NK788_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCH6768786.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8998..10164
                     /gene="dnaJ"
                     /locus_tag="NK788_000410"
     CDS             8998..10164
                     /gene="dnaJ"
                     /locus_tag="NK788_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCH6768787.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10246..10911)
                     /locus_tag="NK788_000411"
     CDS             complement(10246..10911)
                     /locus_tag="NK788_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768788.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10887..11060
                     /locus_tag="NK788_000412"
     CDS             10887..11060
                     /locus_tag="NK788_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768789.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11105..11842)
                     /locus_tag="NK788_000413"
     CDS             complement(11105..11842)
                     /locus_tag="NK788_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6768790.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11844..13346)
                     /locus_tag="NK788_000414"
     CDS             complement(11844..13346)
                     /locus_tag="NK788_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCH6768791.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13605..15599
                     /gene="uvrB"
                     /locus_tag="NK788_000415"
     CDS             13605..15599
                     /gene="uvrB"
                     /locus_tag="NK788_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCH6768792.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15609..18425
                     /gene="uvrA"
                     /locus_tag="NK788_000416"
     CDS             15609..18425
                     /gene="uvrA"
                     /locus_tag="NK788_000416"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCH6768793.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            18688..19953
                     /locus_tag="NK788_000417"
     CDS             18688..19953
                     /locus_tag="NK788_000417"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCH6768794.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            19957..20688
                     /locus_tag="NK788_000418"
     CDS             19957..20688
                     /locus_tag="NK788_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCH6768795.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            20823..21707
                     /gene="rapZ"
                     /locus_tag="NK788_000419"
     CDS             20823..21707
                     /gene="rapZ"
                     /locus_tag="NK788_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCH6768796.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            21704..22702
                     /locus_tag="NK788_000420"
     CDS             21704..22702
                     /locus_tag="NK788_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCH6768797.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            22736..23671
                     /gene="whiA"
                     /locus_tag="NK788_000421"
     CDS             22736..23671
                     /gene="whiA"
                     /locus_tag="NK788_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCH6768798.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24214..24864
                     /locus_tag="NK788_000422"
     CDS             24214..24864
                     /locus_tag="NK788_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768799.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25051..>25423
                     /locus_tag="NK788_000423"
     CDS             25051..>25423
                     /locus_tag="NK788_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCH6768800.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSI"
BASE COUNT         8390 a         4320 c         5444 g         7269 t
ORIGIN      
        1 aaatgattga cttggcaaag aaattcttac acgaaaatat atattttgag ttgtatcatc
       61 atcgagccat ttatacaaat gaagatgctt tggtaataaa gaaagaacaa ggatttacag
      121 gtacagagac caaaagctta ttcttaaaag ataaaaaaga taataactac gtatatctga
      181 cttttactac gaaacaaacg gatttcaaag cattagggaa gatcgtcggt aaaaggcttt
      241 ctattgtttc atcagaacgc atggaagaac aaacgggaca aaagcagggc gctgtttctc
      301 cgtttggtta tgaaaaacag attccgatta ttattgatga agagctctta tcaaatgaaa
      361 aacttgtttt tgcaccaggt agaccagatc aaacgatggt catccaaacg gttgacttaa
      421 acaaaatcat ccagctactt gagatgccga tatttttgat gccggcaaac gaaaaaaaca
      481 attaaccaag taaaatagtg catacttgga ggtgaagcaa catgataagt tatccattat
      541 tagaaagaaa tgcaacgatt ggggttactg caccatcctc aggcgtatca gaggaacacc
      601 attcgttact cgaatcagct ttaaagagaa tggaagaaaa atctttccaa gtagttactg
      661 gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg
      721 aattcaatca aatgatgcaa gatgaaaata tccaaatgat ctttccgact tggggaggag
      781 aattattgat tgagattctc gattttgtcg attttgatgc tctacaacca aaatggatag
      841 taggttattc agatattagt acgttgctac ttgctgtcac attaaaaaca gggatagcca
      901 ctgcacatgg aactaacctt attgatttaa gaggagagta cttggatgaa acgacagcaa
      961 tgtgggaaag tgtactgaaa acaagagaag gagaatcgat tctacagcaa tcttctgaaa
     1021 agtatcaaaa agtgtggccc catgagcatt catcatcttc tgttttccat ctcactgaac
     1081 caacaaattg gcaatcagtc agcggagatg cagtaaggat gaagggacga ttattaggtg
     1141 gttgtattga tacgatcagg catttaatcg gtactcctta tggagatgtc caatctttta
     1201 aaagaaagta taatgatgga aagccgatct tgtggtttct agaaaactgt gagctatcgt
     1261 cagttgattt aagaagatca cttgttcaga tgaaactggc aggctggttt gatggcagct
     1321 cggggatcat gttcggtaga agtgcggcag aggatttgtt agattataaa atgaaggatg
     1381 tatacgaaga tctgtcaaag gaattaaaac taccgatctt gtatgatatt gattgtggtc
     1441 atgtaccgcc acaactgaca ttgattaatg gtgcctatgc agaagtagcg gtagaagata
     1501 gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt ggagtctatc
     1561 aaagaccaat aaatctaaat agaaaagaaa gtgaaagaag ctgacaaaat gctgtcagct
     1621 tctttttgta taacatatta gaaagtctaa ttatctataa ctgtcacatc gattaactgt
     1681 ttcacgtcat tgggatttcc tgtaggaata gctactacag atgcattatc aatgatttct
     1741 tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat ttgccaatgt
     1801 gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac taaaactccc
     1861 tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc actccctgca
     1921 tcataagggg cgataggtgt tcctgcagga taatgaataa catgtaattc gaaatcaccc
     1981 gatgtatctc taaaagtatt acctctaata gctagccaaa cacgtgcgta ctcaatttgt
     2041 agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga ggttgtgtct
     2101 attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga agtattgggc
     2161 tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga tttctgagta
     2221 cttgattttt cagctgaact ggtttgtctg attgaacttt tgtctgtggt cgcagatgga
     2281 tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac caacaataaa
     2341 ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca gtatagcata
     2401 atcctgaatt tttgaatccg taaaaccttg tctatgaata taaatagagt gtatcttctt
     2461 tgataaatgt cagaaaaagt gtgacaaata atcatcagta aaattacttg tcacactatt
     2521 gataaggagt atttaattga agagaatgat taggagaaag gatctccgta ctttttatag
     2581 taatcataga atccaagttt gtatagacga atcgtttttt ctcttatttc ttctgtttca
     2641 aaatggactg cttgaagaat caagtctgtg aattctatag gagctgttcc attcgcagta
     2701 atcaaattac gatctttcac agcttgttgt tctataaaat cttctttatt ctgataattt
     2761 tccgattcta accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg caataatccg
     2821 ctctgagcta aataatcaac tgccccacaa atagcagcta ctggtacgtc tgtttttaac
     2881 aatttatcga ttatttgata taaatgatga ttttttatgt cccatgaatt gccaccgatc
     2941 ataataaaaa ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat ttttgttttt
     3001 aatcctccaa tggaagtgac ctctgattgg atagaagcag ttttgttttg ccatttctca
     3061 ttttggttta gtgtgctagt aatataagca ccttcccaat cggcatattc ctctaataga
     3121 aacgtaatcg cagttttcat tttcaagctc tcctttacta tctataacaa ggacaacagt
     3181 tttaacctac agacattcat taaaattggg tagcgacagc gaaaacaaca attcttaagt
     3241 tttagacaga gtatacagat agtcaactta attaaatacg aactaaataa aaatgacagg
     3301 taatcatttt tgtttaccag cattataaca ttttgcatac atggatgaca aaagaaccaa
     3361 ttcccacttg cttgagtaca atattaaaag aaactggaag gcaaaaagta attatagaaa
     3421 cgaaaaataa atcatatcgg gcactcagac atgaaaacaa ccctagagat atataactag
     3481 gtaaccagtt taatgaaaga aaaaatcatc caagaagtcg attcttggat tttttagtgg
     3541 atttgttcgc caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa agcgttgtgg
     3601 tctgagcact taaggaggaa tctgtgaaaa tagcttttca tatttttgca gattttgact
     3661 taatgccgaa ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa gcaccgtgta
     3721 gctccgatga caataagcaa gcatttttag agctaggttt ccttgattct aaatatgcgg
     3781 cttatttcaa tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac aggcacgact
     3841 tttcgacgta aggagaaaga atttcaatga tagaaacaat aaatcccgca gaaaacaagg
     3901 tttctgcgta cattcatatc ccattttgcg agcatatctg ctattactgt gattttaata
     3961 aagttttttt agaagggcag ccagtagatg agtatgtgga aatgctgctg aatgaaatga
     4021 aaataatgct agaaagtcat ccgacagcga atttagaaac actttacgta gggggcggga
     4081 caccaacttc tttatctgct aaacaacttg atcgactgtt atcaggtgct agagagatcc
     4141 tgccttttca aaatggtaat gagtttactg tagaagcgaa tccaggtgat ttgacccgtg
     4201 aaaaacttca agtaatgaaa aactatggcg tcaatcgttt atccatggga gtgcagacat
     4261 ttgataatcg cttattgaaa aaaattggac gtaaacacac cgcagaagat gtgtatcaaa
     4321 ctatgcaatt tttagaagca gaaaatttta ccaatgtaag catcgatttg atttatgcat
     4381 taccgggaca aacactagaa ggatatctgg atactttgga aaaagctttg gctttggatc
     4441 tgcctcacta ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc atgaactggg
     4501 tacgacaagg aagactcgaa ctgcctgatc aggaaacgga aaccagaatg tttgaagaga
     4561 cgattgaagc aatggaaaag catggacgtc accaatatga aataagtaac tttgggctct
     4621 ccggacatga atctcaacat aacctcatgt actggaacaa tgaccactat tttgggtttg
     4681 gagctggtgc tagcggttat cttggagata ttcgttaccg caacaaagga ccaatccaac
     4741 actatttacg cccattacga gcaaatgaac gcccggtatt ggaagaagaa aaattgacac
     4801 gaaaaaatca aatagaagaa gaaatgttct taggactaag aaaaaaaaca gggattacta
     4861 aaaaacattt ctatgatcgc tatcaaaaaa caattgaatc ggtgtacggt gatgtgctaa
     4921 gggaattaga gcaagaaggg ttacttgtta atgaagaaga ccgtgtttat ttgacaccaa
     4981 gagggacctt tttggggaac aatgtgtttg agcgcttttt gtttgaaaaa tagtttaaag
     5041 aatatttgtt ttttatctga atttaaataa aaaaagttca aaaatacaat aaaggactat
     5101 tgaaacgttg atataactgg atttacatca attatttaga attagcactc ttgtgtttag
     5161 agtgctaaaa aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag
     5221 tggattagca cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg
     5281 acaaaacgac attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg
     5341 ttccaaaaca ttgatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat
     5401 gaaagcatta gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc
     5461 atctgcttta ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga
     5521 agatgatttg ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat
     5581 tattaagcaa tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg
     5641 acctgaaata aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca
     5701 gattattgct atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc
     5761 cgaatatatg ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt
     5821 cggagatcct ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca
     5881 taaatatttc caaacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt
     5941 ttttgaagat aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca
     6001 agatcttgat cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca
     6061 aatgatcact ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga
     6121 tttattgcag aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg
     6181 tacaatagcc ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga
     6241 tgtttatcga agagaactag cagctaaact tgctgattac taccgttcgc ttgacttgtc
     6301 aaactcgtaa tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag
     6361 agcaatcaaa cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga
     6421 agaactagat aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc
     6481 agaagattcg ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc
     6541 tgagttagaa gaaatggaag ataaatactt gcgggctcgt gctgaaatcg caaatatggc
     6601 aaatcgagga aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa
     6661 aaaattactt ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga
     6721 tcaaggggca ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact
     6781 ggaagaagaa ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca
     6841 tcaagcagta caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt
     6901 actacaagaa ggctacaaat tacatgatcg cgtgttaaga ccaaccatgg tcattgttgc
     6961 acaataattg tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg
     7021 agtaaaatta tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga
     7081 aacgaagcaa aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca
     7141 ttcaaaaatg gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca
     7201 aatacgattt catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa
     7261 ggtaaatcat atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc
     7321 gcagaagact atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt
     7381 aatgatgcac aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa
     7441 cgtatcgtca atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc
     7501 gacgaaaaaa tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa
     7561 ctaggagacg gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac
     7621 gactttgata acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt
     7681 gacttgtctc aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa
     7741 aaagatttat ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa
     7801 tctggaccat tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct
     7861 gacttagtag aacgtacaaa agtaccagta cgtcaagcgc taaaagatgc aggtttatca
     7921 caatcagaaa tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta
     7981 gaagcagtac gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta
     8041 gttgctatgg gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt
     8101 ttacttgacg ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa
     8161 ttgattgacc gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca
     8221 gataatcaac cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac
     8281 aacaaaactc taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca
     8341 caaatcgaag taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat
     8401 ctaggtacac aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagacgaa
     8461 gaaatcgaac gtatggtaaa agatgcagaa gcaaatgcag aagcagataa agctcgtaaa
     8521 gaggaagttg acctacgtaa tgatgtagat gcattgctat tctctgtgga caaaacattg
     8581 aaagaattag aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgcgat
     8641 gaattgaaag ccgctgttga agcaaacaac atcgaagaaa tgaaaactaa acgcgatgca
     8701 ttgaacgaaa tcgttcaaaa cttgactgtt aaactatacg aacaagcggc acaacaacaa
     8761 gctcaagaaa acccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt
     8821 gaagaagtcg atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt
     8881 tgctactttg gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa
     8941 aatatgatat gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg
     9001 gcaacaaaac gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa
     9061 ataaagaaag cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct
     9121 gatgcggaag aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa
     9181 aaacgcgctg catatgacca atacgggcat gcaggcacag atccaaatta cggtgctggt
     9241 ggagctggtg gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt
     9301 gaagatatct tcgactcttt ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct
     9361 cgtcaaggtg ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt
     9421 gtagaaaaag aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tgggaacggt
     9481 gcaaaaccag gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgattaat
     9541 gtagaacgcc aaacaccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat
     9601 gggacaggga aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag
     9661 aaagcacatt cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt
     9721 ttagctggtc aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtc
     9781 ttccgtgtag aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg
     9841 cctttaagtt ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga
     9901 gatgtgaaat tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga
     9961 aaaggcgctc cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg
    10021 atcacaccga aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct
    10081 ggcggcatca aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct
    10141 tttggcggga aaaagcggaa gtagtttttc tagtaaaaac gctaagtcat taataattaa
    10201 aaaccagacg aacaatcata tgttcgcctg gtttttttgt agttgtcatg tttgtaccaa
    10261 caaaaaagcc agctttagaa tatgataatc gtcatagttt ctaggggtat aaccagtgag
    10321 agaatggaac ttgttcaatc gatattgcaa ggtgttttta tgaataaata attcttctga
    10381 gcaatttttg atgcttccgt tatttttact gaagcatgcc aaagtcttct tgaacaaatg
    10441 tacttcttct tctgtcaggc ttttcaaaat acgttttaca tataatgttc gttgatctga
    10501 cgggattgct ggtaaaacga tcccgaggtc taaatcttca aaaaagcgga tctcgttcac
    10561 agattgatgg aaagctatcc attcgcaaac ttgtttggca tgaaggaaag attgcggtgt
    10621 ctcttgctct gtataacaaa gcgacccaat tccaaagttt agctgtaact gatacatttt
    10681 attaaacttt gtctggattt tatcaagcaa tgatagcaat gaggctctgg aaatccgatt
    10741 gactaggata atgatttgtt gtctatgtac agtgagcaaa gcctgtccgt aataagagaa
    10801 gaccctttcg agcaactcca ataaaggatt gatccgttct tgacacaatt gctgattgtc
    10861 agaaagttgt cccacaacta cataatatgg agaagaaaaa tcaagctcaa ttaagtgagg
    10921 gtggtatcga ctatgtactt gatatcatca atgaaccgat ggtactttct tatgcgatgg
    10981 aacaggtaga gcgattgctt gtttgtgcag gaaaacaagc gatgcggctg atacgattag
    11041 gtgagaaaat aagaagataa aaatacacgg tcccttcatg gcgaccgtgc atttttggtt
    11101 tttattaaat atttaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
    11161 caaagatttg ttgaggcgtt ccatcttcta aaaagtttcc gtcgtcaatg aacatgacgc
    11221 gattggccac ttctttagcg aaccccattt cgtgcgtaac gataaccatc gtcatacctt
    11281 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcagga tcgagtgcag
    11341 aagtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
    11401 cacgctgttt ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac
    11461 caactgtctg cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
    11521 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
    11581 gttggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
    11641 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
    11701 gcaagaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
    11761 tgatggatac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
    11821 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
    11881 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
    11941 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
    12001 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
    12061 atgcgtatcg cttgcggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
    12121 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
    12181 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
    12241 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
    12301 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
    12361 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
    12421 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
    12481 agaaatgttg attcatcagc agtggaagac tcactggaag tttctgaacc atccgctaca
    12541 tattctttaa taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
    12601 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
    12661 gattcttttt tgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
    12721 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
    12781 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
    12841 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
    12901 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
    12961 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
    13021 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
    13081 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
    13141 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
    13201 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
    13261 tcagcttgtg caacatgacc aaacgcaaat gtcacaagtc ctgtcattaa gaaaacaaat
    13321 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
    13381 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
    13441 gtttctaaca ttttatgtta taaaacataa catgtataat aagataaaaa agtggcgaaa
    13501 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
    13561 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
    13621 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
    13681 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
    13741 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
    13801 tgattattgc ccataacaaa acattggctg gtcagctgta cggggaattc aaggaatttt
    13861 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
    13921 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
    13981 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    14041 ccgtttcatg tatttttgga ttaggctcgc cttttgagta ccaaaaacaa gtagtttcga
    14101 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    14161 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    14221 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    14281 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    14341 tagcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    14401 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    14461 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    14521 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    14581 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    14641 acgtaaccat gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    14701 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    14761 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    14821 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    14881 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    14941 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    15001 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    15061 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    15121 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    15181 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    15241 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    15301 gtctagcgat ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac
    15361 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    15421 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag
    15481 aaaaagacac gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg
    15541 attttgaaac agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    15601 aggacaacat ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa
    15661 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    15721 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    15781 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    15841 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    15901 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    15961 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    16021 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    16081 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    16141 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    16201 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    16261 gcctatttga ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg
    16321 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    16381 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    16441 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    16501 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    16561 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    16621 aattaccgga agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc
    16681 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    16741 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    16801 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    16861 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    16921 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    16981 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    17041 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    17101 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    17161 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    17221 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg
    17281 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    17341 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    17401 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    17461 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    17521 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    17581 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    17641 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    17701 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    17761 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    17821 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    17881 ttccttgtga agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca
    17941 aaggaaaaag cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca
    18001 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    18061 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    18121 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    18181 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    18241 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    18301 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    18361 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    18421 tataaagagg atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg
    18481 ttttattaat atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta
    18541 tagataaaaa cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact
    18601 ttcctttgat tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa
    18661 caagagatta tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc
    18721 cctatattat taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca
    18781 tacggcaaaa tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa
    18841 attgtcaatg tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg
    18901 cttcgtttgg caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt
    18961 ggtgatggat atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc
    19021 tgtccatata ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa
    19081 gtttgtgaag aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta
    19141 gtaaatggac atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca
    19201 aacagtgtgg agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag
    19261 actagagaag agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa
    19321 atgattgtac aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat
    19381 gtagatgaag atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct
    19441 acaccagcat cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat
    19501 caaaccatta aggcattttt ggaaaaaatc gaatatacag gctttgccaa tttcgatatg
    19561 aagtatgatc ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt
    19621 agcagtttct ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga
    19681 gtgttcaaca aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga
    19741 ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa
    19801 aaactagctg agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa
    19861 gaccgatcaa tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga
    19921 ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta
    19981 ggcggaatgg gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa
    20041 gctacaaaag atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat
    20101 cgtacagctt atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat
    20161 attgagaaac aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac
    20221 tacttttttg aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa
    20281 gcagcaaatg aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa
    20341 ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg
    20401 attcctgata ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa
    20461 tcggattatt tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta
    20521 aactgtgaaa aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa
    20581 gataatcatt acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt
    20641 gcattagctg aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt
    20701 tggggcaaat atcctaaaag atatttggtc caaatctttt tgtaaaagaa aaagaaaact
    20761 ggtaatgata cgcccgggat gatataattt tattatgcac aatgtgagag gggttcgtaa
    20821 atatgacaga taatttgcaa ttagtcatca taacaggtat gagtggtgca gggaaaacag
    20881 tagctatcca aagttttgaa gatatgggct atttttgtat cgataatatg ccgcctcgat
    20941 tgattccaaa attctgggaa ttgatcaaag aatcaggtaa agtcacaaaa atggctttag
    21001 gagtggattt gcgttctcgt tccttctttg aagaaatcca aaacatgctg atcgaaatcg
    21061 aaaatacgaa cttcatcgac acttctatct tatttttaga tgcttcagac gaagaactag
    21121 tggctcgcta caaagaaaca agacgagcac atccaatggc gatggacggt ttgatcactg
    21181 aagggatacg taaagaacgg gcgatactag acgacttgaa agcccaagct tccgtgatca
    21241 ttgatacgac aacattgaca ccacgacaat tacgtgaaaa aatcaatgag tcattcaaat
    21301 caaagggaga ccaaggattt cggatcgaat ttgtatcatt tggttttaaa tacgggttgc
    21361 cgatcgattc ggatattgtg atggatgtgc gttttttgcc caaccctcat tatatcccgg
    21421 aactaagacc attgaccgga aaagatcagc cagtctatga ttatgtgatg tcctttgatg
    21481 aaacgcaatc attctaccat aaatttttgg atcttctgga aactgtattg cctggataca
    21541 ttaaagaagg aaaaagcagc ttgacagttg caatcggctg tacaggcggt caacaccgtt
    21601 ctgtggcatt gactgaacgt ttggcaaatg ctttagggaa aaactataaa gtcaatgtga
    21661 cacatcggga caaggataaa aggaaagaga cggtgaaccg gtcgtgaaaa tgaaaaccta
    21721 ccggataaga aaaccaaaga tcgtagtggt cggcggtggc actggtctgc cagttatcct
    21781 gaaaagttta cgaaatcaag gagcagatat cacagcagta gtgactgttg cagatgatgg
    21841 tggaagcagc ggtgcaatca gagaatcgat cgctatggct cctcctggtg acttgagaaa
    21901 tgtcttagtg gcgttatcgg atatgccaca attctatgaa gatattttcc aataccgttt
    21961 caaaaaggaa gatcagtttt tagccaatca tacgatcggt aatctgatca ttgccgcagt
    22021 ttccgagatg cggggaagca cttatgaggc gattcaatta ttagcaaaaa tgatgcatgt
    22081 agatggacat gtttatcctt cttcagaaac tcctttgacc ttgcatgctg tttttaaaga
    22141 cggaacgact gcagtaggag aatctaaaat cgcgatcaac cgtaaaacca ttgaccgtgt
    22201 gtatgtctgc aacacaaatg ataaaagtga agccaaagcg gctcgaaaag tcgtttcagc
    22261 aattatggac gccgacatgg tcgttttggg acccggaagt ttattcacca gtatccttcc
    22321 aaatttagtg ataccagaaa tcggcgaagc aatgctcaag acaactgctg aaaccgtcta
    22381 tatctgtaat atcatgaccc aaaaagggga aacggaacat ttcacagatg ccgatcatat
    22441 acgtgtttta cacaagcatt tgaatcagcc ttttatcgat acagtattgg tcaatacgga
    22501 aaaagtgcct gaagattata tggacccaga gatctatgat gagtatctag tacaagtcgc
    22561 acacgacttc aacggattga gaaatgaagg atgtcgagtg atttccaccg actttttaga
    22621 attgaaagat ggtggtgtct tccacgatgg agacaaagta gtagaagaat tgttccgttt
    22681 agttttcggt tcaaaatatt aagcaattta ttgttggaga aaggaggaga ataggatgtc
    22741 atttgctgca gatgtgaaga aagaactaac tggtttggaa gttcatcgag aacatgcgaa
    22801 agcggaatta gctgctttgc tgcgaatgaa cggctcttta ggtattgtga accagcagtt
    22861 tattttgaac gtccaaacag aaaatgctgc aattgccaga cgtatctatt ctcttttgaa
    22921 agatcattat caggtacgaa gtgagctact cgttcgtaaa aaaatgaaat taaaaaagaa
    22981 caatgtctat atcgtccggt tgaagcaagg cacaaaagat gtgttgatgg atcttgatat
    23041 catggatggt atgatgttca atggacacgt ttcggacgag attatgggaa attcgcaaaa
    23101 aatgcgttct tacttacgtg gtgcttttat ggcctcaggt tcagtcaata atccggaaac
    23161 gagtcgttat catttggaaa tattttctat ttatgaacag cataaccaag acatttgcga
    23221 tatgttaaat tattacggtc tacatgctcg aacgcttgaa cggcgcaatg gctatatttc
    23281 gtatttaaaa ggagcagaaa aaattgctga tttcttaaca ttgattggtg caaccaactc
    23341 aatgctccga tttgaagatg ttcgaatcgt acgggatatg agaaattcgg tcaatcgtct
    23401 agtcaattgt gagacagcaa atctgaacaa gacgattgat gcggcttcta aacaaatcga
    23461 aaacatcgaa ttgatcgaag caaaggtcgg tctacatgcc cttcctgaaa aacttcagga
    23521 aatcgcagaa cttcggcttc aacatccaga ggtcagcttg aaagaattag gggaaatgat
    23581 tccctcaggt gcaatatcaa aatcgggaat taatcaccga attcgcaaaa taaatgaatt
    23641 tgcggaaaaa ctgcgtgaag aagcagttta atccaaagaa aaaactgata catgaagcag
    23701 gagatgcgaa tgtatcagtt ttttttaaaa gctatacttg cttttcttcc gtcaaactat
    23761 aattccaact ttactggagt aacataatgt tgaaaggctt ctattgtttt ttaatacaat
    23821 cgtataaaaa gcggggagac aactgaatga agattttagt tttatacata aacttttgct
    23881 taatatagaa gtcctgatat agataggatc aagtatttat ctataactgg agattaatta
    23941 aaaactggaa ggaactgctc gatcaacctt gagaactgtt tttcttactt ctggtcaaga
    24001 ttctcgtggt tggtggataa ggatggttga taagttcatt ggattagcta gtcgaaaaca
    24061 accaggtgta attaatgtag aagttttaac aaggggatgg agtgatttat tcagtgataa
    24121 tcataagttc ataaatagta attgggatgt atggttggta tacgttatac ctgaacttga
    24181 actatcaaga tttggtttag aatttgagaa tcgatgttta taaagggagg gcaaaaaatg
    24241 tgctttttta caacttttac tatcattatg attgcagtac tttttatttt tctagatata
    24301 gcaaagcgaa atacagcatt tttattatat cgggttttac ttcgagcagg tctcataaca
    24361 tttatatcga tcgttggatt ttttctattc acagttattg tatttatttg gagaactaca
    24421 gcaccaccat tgccagagat tacatatgga gaatttccgt ttagtcttga atatgaaata
    24481 gatgaggaaa gaattttgat tgaagatacg gtgattgcag aatttatagg aagttttaga
    24541 gggaatgtga caactcaacc tagacgaagt tggagtataa aacttttaaa taatagtgga
    24601 gagttgaaag catttcatta tgaattctta ataatagaaa gtgaagatgt aacaatcact
    24661 ttaagtagtg gctctgctgg atattttatg aatgatggtg gaagcaagcc acagctcaca
    24721 gtccgtgatc gtattgcagg ttttatgcat ttttctcact atgacttaga agaagctcgt
    24781 aaaatacttg cagaatatgg gatcacatta atcaattggg aatatgcgcc accgataaga
    24841 aattttttca atgattatta ttagtcttat aaaaaacaga atgtaaaatt tatgtgcgcg
    24901 agtatttagt tttccatttt ttgtttgata gatatatagc attgttgaag catgggatag
    24961 tggtgccaat tttatcaatg aggttggtca ggcagttaac ataattggtg atgctataaa
    25021 tccatttaat tggaattgga ggaattaggt gtggaaacag tgagattatg gatattcatg
    25081 aaaaattttc gttcggcttt gagatatgtt attctggaat caaaatcagg ttattatctg
    25141 attgacacag atcactatac tttttgggga ggctaccttt ttccgatttt taattggata
    25201 atcccccata aggttatgaa aattaacttg cctgcagaag aaataaatag attaatactc
    25261 aacaaagatt tacaaaggaa aagagagaaa gaacattcta atgttgcttg gataggtcca
    25321 gcatttgcag tagtgacttc ttcaattgtc ggtcctatgc tagagagtat ttatagtgat
    25381 ttttctattg gaatacgtta tttatttgtt actttaagta tag
//
LOCUS       DAHUWM010000015        25213 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHUWM010000015 DAHUWM010000000
VERSION     DAHUWM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.015"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            12..84
                     /locus_tag="NK788_000424"
     tRNA            12..84
                     /locus_tag="NK788_000424"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="NK788_000425"
     tRNA            88..160
                     /locus_tag="NK788_000425"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="NK788_000426"
     tRNA            168..249
                     /locus_tag="NK788_000426"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="NK788_000427"
     tRNA            267..339
                     /locus_tag="NK788_000427"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="NK788_000428"
     tRNA            351..422
                     /locus_tag="NK788_000428"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="NK788_000429"
     tRNA            439..524
                     /locus_tag="NK788_000429"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="NK788_000430"
     tRNA            535..608
                     /locus_tag="NK788_000430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="NK788_000431"
     tRNA            618..691
                     /locus_tag="NK788_000431"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="NK788_000432"
     tRNA            711..783
                     /locus_tag="NK788_000432"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="NK788_000433"
     tRNA            811..884
                     /locus_tag="NK788_000433"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="NK788_000434"
     tRNA            898..971
                     /locus_tag="NK788_000434"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="NK788_000435"
     tRNA            995..1084
                     /locus_tag="NK788_000435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="NK788_000436"
     tRNA            1097..1170
                     /locus_tag="NK788_000436"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="NK788_000437"
     tRNA            1189..1264
                     /locus_tag="NK788_000437"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="NK788_000438"
     tRNA            1266..1336
                     /locus_tag="NK788_000438"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="NK788_000439"
     tRNA            1350..1423
                     /locus_tag="NK788_000439"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="NK788_000440"
     tRNA            1448..1535
                     /locus_tag="NK788_000440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="NK788_000441"
     tRNA            1557..1628
                     /locus_tag="NK788_000441"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="NK788_000442"
     tRNA            1651..1740
                     /locus_tag="NK788_000442"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="NK788_000443"
     tRNA            1753..1826
                     /locus_tag="NK788_000443"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="NK788_000444"
     tRNA            1845..1917
                     /locus_tag="NK788_000444"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="NK788_000445"
     tRNA            1921..1993
                     /locus_tag="NK788_000445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="NK788_000446"
     tRNA            1998..2068
                     /locus_tag="NK788_000446"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="NK788_000447"
     tRNA            2081..2154
                     /locus_tag="NK788_000447"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="NK788_000448"
     tRNA            2166..2237
                     /locus_tag="NK788_000448"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="NK788_000449"
     CDS             2503..3696
                     /locus_tag="NK788_000449"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCH6768801.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="NK788_000450"
     CDS             3826..5298
                     /locus_tag="NK788_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6768802.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="NK788_000451"
     CDS             5381..6169
                     /locus_tag="NK788_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCH6768803.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="NK788_000452"
     CDS             complement(6236..6904)
                     /locus_tag="NK788_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCH6768804.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="NK788_000453"
     CDS             7019..8731
                     /locus_tag="NK788_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768805.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="NK788_000454"
     CDS             8734..10503
                     /locus_tag="NK788_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768806.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="NK788_000455"
     CDS             complement(10708..11274)
                     /locus_tag="NK788_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6768807.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="NK788_000456"
     CDS             complement(11274..12230)
                     /locus_tag="NK788_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCH6768808.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="NK788_000457"
     CDS             complement(12230..12886)
                     /locus_tag="NK788_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCH6768809.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="NK788_000458"
     CDS             13256..15274
                     /locus_tag="NK788_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6768810.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="NK788_000459"
     CDS             15701..18115
                     /locus_tag="NK788_000459"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCH6768811.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="NK788_000460"
     CDS             18463..20016
                     /locus_tag="NK788_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCH6768812.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="NK788_000461"
     CDS             complement(20397..22181)
                     /locus_tag="NK788_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768813.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="NK788_000462"
     CDS             complement(22196..23917)
                     /locus_tag="NK788_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768814.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="NK788_000463"
     CDS             24017..24778
                     /locus_tag="NK788_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6768815.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7801 a         4632 c         5365 g         7415 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tcc
//
LOCUS       DAHUWM010000016        25115 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHUWM010000016 DAHUWM010000000
VERSION     DAHUWM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.016"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..340)
                     /locus_tag="NK788_000464"
     CDS             complement(<1..340)
                     /locus_tag="NK788_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768816.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLH"
     gene            607..2307
                     /locus_tag="NK788_000465"
     CDS             607..2307
                     /locus_tag="NK788_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCH6768817.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2277..2495
                     /locus_tag="NK788_000466"
     CDS             2277..2495
                     /locus_tag="NK788_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCH6768818.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2515..3405
                     /locus_tag="NK788_000467"
     CDS             2515..3405
                     /locus_tag="NK788_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCH6768819.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3423..3707
                     /locus_tag="NK788_000468"
     CDS             3423..3707
                     /locus_tag="NK788_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768820.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4194..4808
                     /gene="gmk"
                     /locus_tag="NK788_000469"
     CDS             4194..4808
                     /gene="gmk"
                     /locus_tag="NK788_000469"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCH6768821.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4813..5118
                     /locus_tag="NK788_000470"
     CDS             4813..5118
                     /locus_tag="NK788_000470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCH6768822.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5371..7779
                     /gene="priA"
                     /locus_tag="NK788_000471"
     CDS             5371..7779
                     /gene="priA"
                     /locus_tag="NK788_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCH6768823.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7794..8285
                     /gene="def"
                     /locus_tag="NK788_000472"
     CDS             7794..8285
                     /gene="def"
                     /locus_tag="NK788_000472"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCH6768824.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8278..9216
                     /locus_tag="NK788_000473"
     CDS             8278..9216
                     /locus_tag="NK788_000473"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCH6768825.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9216..10574
                     /gene="rsmB"
                     /locus_tag="NK788_000474"
     CDS             9216..10574
                     /gene="rsmB"
                     /locus_tag="NK788_000474"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCH6768826.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10587..11327
                     /locus_tag="NK788_000475"
     CDS             10587..11327
                     /locus_tag="NK788_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCH6768827.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11324..13393
                     /gene="pknB"
                     /locus_tag="NK788_000476"
     CDS             11324..13393
                     /gene="pknB"
                     /locus_tag="NK788_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCH6768828.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13558..14457
                     /gene="rsgA"
                     /locus_tag="NK788_000477"
     CDS             13558..14457
                     /gene="rsgA"
                     /locus_tag="NK788_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCH6768829.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14470..15120
                     /locus_tag="NK788_000478"
     CDS             14470..15120
                     /locus_tag="NK788_000478"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCH6768830.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15121..15759
                     /locus_tag="NK788_000479"
     CDS             15121..15759
                     /locus_tag="NK788_000479"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCH6768831.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15914..16768
                     /gene="mreC"
                     /locus_tag="NK788_000480"
     CDS             15914..16768
                     /gene="mreC"
                     /locus_tag="NK788_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCH6768832.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16772..17281
                     /gene="mreD"
                     /locus_tag="NK788_000481"
     CDS             16772..17281
                     /gene="mreD"
                     /locus_tag="NK788_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCH6768833.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17536..19074
                     /locus_tag="NK788_000482"
     CDS             17536..19074
                     /locus_tag="NK788_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCH6768834.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
     gene            complement(19142..20941)
                     /locus_tag="NK788_000483"
     CDS             complement(19142..20941)
                     /locus_tag="NK788_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCH6768835.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21064..21570
                     /locus_tag="NK788_000484"
     CDS             21064..21570
                     /locus_tag="NK788_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCH6768836.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21649..22371
                     /locus_tag="NK788_000485"
     CDS             21649..22371
                     /locus_tag="NK788_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6768837.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(22449..23849)
                     /locus_tag="NK788_000486"
     CDS             complement(22449..23849)
                     /locus_tag="NK788_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCH6768838.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(24019..24993)
                     /locus_tag="NK788_000487"
     CDS             complement(24019..24993)
                     /locus_tag="NK788_000487"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCH6768839.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
BASE COUNT         8166 a         4628 c         5328 g         6993 t
ORIGIN      
        1 aatgaagatg tgttttcata tgaccaaaat ataataattc tgaaatttct aaaggtgtca
       61 attctttcag catcgttaaa tcagtaaagt cgatccactt caattcatcg aatccacgcc
      121 tgttacgaga agaggaaaag aaacgctgaa tattttctgt accacggatt actttcagcc
      181 ctgtatggga ttcatattct ccagcagaag cagatggatc aagcaagagc aagttttgag
      241 gaaaatgcac gatactgttt gcaaagtcag tagcagtcag cccttttgtt aaaacagcat
      301 tacttgtggt atcaatatgt acataaactg actcagtcat catgttcctc ctcttatttc
      361 ctcttaatct cttttatttt acaacatttg cattaaaaaa gcttgtttct tttattttaa
      421 aattccatta caaaatcacg ttttcattga aaacactctc attttcaatt aatttttgtg
      481 atttttgtat ttttttattt aaaggaggtt ttatgaattg ttttcttatg gaatctacaa
      541 agtcgaatac gagactcatg acctttttat gacgtactgg tatacttgaa gaaaaaggag
      601 agcgcgatgg agataaaaaa agtttacttt tcacagaaaa tgtcgctgta ttcgcctatc
      661 cagcaagcaa aacaacccct tccatttttt caaagtcatc aagaaagtag agacgctttt
      721 tggcgttctg caaaaaagca atgtgctttt aaagcagtat cgaaaaaata taccgtctat
      781 tttactattg ctaaaagtag aggagatgtt atttttaata atctgttgat tgaaggtgaa
      841 acttttgaat ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt ttttattaaa
      901 gaaaactgtt gttttttatt tcaatctcct ctatcagaag aatggttgcg tatttttcat
      961 aaaaacggct atcaaggaac cacacaatta aataaaaagc tggtctatca tacagcactt
     1021 gttttaggtg gaggaggagc gcacggtgct tatcagattg gtgtgtggca ggcgctgaag
     1081 gaacatgata tcaactttga gattatcaca ggtacctcgg taggtgcgtt gaacggcgca
     1141 ttgattttac aaggagatat gaaaaaagct gtgaatttat ggaaaaagct gtcgacaaga
     1201 caagtgcttg cgttaccaga aatggctgca gttgaagacc ttcgtgaacg cttctatcga
     1261 gaaagacgcc aaatgacgaa aacagcacta attgaaggcg gggtttcttc tgctccattg
     1321 gaaaagatgg tcaaagacaa tttggatttg tccttactca aacataatcc caaaatacgt
     1381 ttatatacgg tgtctactaa actgccagaa ctagcggaag tagtgactga tatccaacag
     1441 acaaaaacag aagaaatacc agattggata ctcgcctctg cttcatttta tcccgctatg
     1501 gcttatcgaa agatcggaaa aaataaatat gcagacggtg ggtatcgcaa caaaatacct
     1561 attgatattg cgattaacaa aggagcaaca gaggcatttg tcgtagatgt gcaagggcca
     1621 ggcccagcga aaaaaattcg tatgccagac atttttatcc attggaaatg tcaaacattg
     1681 tggacactag gcagctttct gctttttgat agccaaagga accaattaaa tttacaatta
     1741 ggctatctgg aaatgaaaaa acgattaggc tattactatg ggaactggta tacatttcat
     1801 tctgttaaac ctgcaagaac atactggcga ggatttttgt cttacttaac aaaagaaatt
     1861 cagttagaat tatctttttt caaaagcata aaattttggc aaaaactgcg taatttatat
     1921 aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa
     1981 cattttttac tgcccaatga aatctatcaa gtagacatga tgattcaaat gatctgccaa
     2041 caagatataa aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt aagtactgaa
     2101 gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt
     2161 tattttgatt atctgctaca aggaaaacgc aaggatcgtt tgcagcatga attgcttaag
     2221 caaccagttg acacattact gatcctatat ttatattatc tcaaggagga acaaccatgg
     2281 cacaagaatt ttcatatgaa atcgtagaag aaatcgctat attgtcagaa aacaacaagg
     2341 ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc
     2401 gagattgggc gccagaccat gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt
     2461 ttgaacaact gcaaaaagca atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa
     2521 agtatgactg gttttggcaa agccatccgc gaaacaaaag cgtatcagtt agatattgaa
     2581 ataaaaagtg tcaaccagcg ttttctagat gttcaaatac gaagtcctaa aatcctgaac
     2641 tttgttgaaa acgattttcg tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa
     2701 gtatttgtga atctagctta tattggcaaa aacgaaaaac agatcgtcat caactgggaa
     2761 ttgattgatt cacttatcac tcaattgaca gacggaattc aacaacgata cggtagtcaa
     2821 aatcaagtgt caattagtcg gatggcggaa cgcttcgtgc tggacgaaaa cttcgtaacg
     2881 gtagaagaaa aaccggaaga gagcatggaa gaattggcac agttagctct agatacattg
     2941 aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag aaggtcaagc tttagcttta
     3001 gtgttgaaaa agaatactga agaattcaaa gaagtactaa aaaaactcga tagttttgta
     3061 gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa agctggagga atttcttgga
     3121 gcgaaagtcg atgagcaacg gttgttgacg gagctagcca tcttgttgga acgcggagat
     3181 attcacgagg aactggatcg tctggcgatc catgtacaaa aactagatga acttatcgaa
     3241 gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc aagaaatgaa tcgggaaatc
     3301 aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa atcaagtcgt ccagtctaag
     3361 acgatcttag aaaaaatcag agaacaagta caaaatattg aatgacgaaa gaggtagaga
     3421 taatgaaata ttatgacgta acgttccacg aattaagtgg aaaaactgtg gttaaacggg
     3481 atatcccttc tgaaaaaaac ggctttgacg tatggaaaga tgcctgtgca gactataatg
     3541 agaacgaact gtttattttg atcaacgacg gtgcttacgt gacgatgaat cggaaattta
     3601 ttgttcgaat tgacacagaa gaggtagaag atccgacaga aaaagcacgt tctcgaaaag
     3661 atgagatcat gggtgtagtg aatactttgt ccaatatggg cttttagcaa ggatgctgaa
     3721 caaagaaata caataaataa acagaattgg atttcttcgc ttagcttctt tgctctagga
     3781 atacatttaa aggttgtgac aaaattcgtg tcacaatctt tttttatttt gaataagtga
     3841 tgttcaaaaa ccattttctc ggtattaagc caaatttatg caaaaatgtg agaaactatt
     3901 ttcacaaaat ttttcttcat actcgaaact aatcggcttc ttcacgacct cttatacacg
     3961 gcgttcaaaa gcttgcatct gcgataataa ccccaaccaa acgaaaaaca tgagaagctg
     4021 tttatgtttg gctgttctta ttacttgatg caggacagct tttttacgac ctcttggtac
     4081 agggtagctt ttttaccaat ttctagctat aattaaagat aaccttgaaa gttgcagaaa
     4141 aaaagtgcaa aataaacgat aagtacaaaa agactagttg aaaggacgtc gtcatgtcag
     4201 agcgtggatt attaatcgtg ttgtctggtc catccggtgt aggaaaaggc acagtacgaa
     4261 aagcaatttt tgagagtgag gataatgatt ttcagtattc gatctcaatg actacccgta
     4321 aaatgcgtga aggggaggtc gaaggcgttg attatttctt ccgaagcaaa gaagagtttg
     4381 aatcaatgat cgaagctgga gagatgctgg aatacgcaga atatgtcggg aattattatg
     4441 gaactccttt gtcatatgtc aacaagacat tggatgaagg aaaagatgtt ttcttggaga
     4501 ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc cgatggtgta ttcatctttt
     4561 tgactccgcc agatttagcg gaattaaaat cacggatcgt aggccgagga acagatgcag
     4621 atgaagtaat cgaaaaacga atgaaagttg caaaagaaga aatcgaaatg atggctcttt
     4681 acgactatgc tgttgtaaat gatgaagtgc cgctagcagt tgaacgaatc aaaaacatta
     4741 ttgccagtga acattttcga gtagatcgcg taatcggtaa atatattaaa atgttaaagg
     4801 agatgtagtc ttatgatgtt aaaaccttca attgactcac tattagatcg tgtaaattca
     4861 aaatattctc tagtgatttt agcaagtaaa cgcgcgcatg aattggatgc aagtgcacag
     4921 cctactctag attcttttga ttcagtaaaa agcgtcggtc aagctttaga agaaatcgat
     4981 gcaggaaatg tagtcaatga tccacatcct gaattaaaac gtgaacgttt gaagatggaa
     5041 gaagaggaac gtcaagcaca aaaagagcgt gaacaacacg aattggaatc acgtatccgt
     5101 gaagagcaaa aaatgtaata aagaaaaata gagcgaacat ctaaaaagaa aagcaaaccc
     5161 agctgtttcc ttttcaaaca ttcgccactt attgtctggt agaaaaggct gtgtctgaac
     5221 tttttgacac agttcttttt tttcttatct gctgttaatt ttttgttagg atttggcaga
     5281 agataagaca aatccatcga tctctagtaa aataggcaag gaactttttg tcgcttgata
     5341 agaaaagaca aaaaaaggag gaaacgaacc atgacattaa cagctgaagt cattgtggac
     5401 gtacctacta tgcagaccga tcagccattc acttatttgg ttccctctga aatggaaaca
     5461 gctttgcaag tcgggatgcg agtagaagtc ccttttggta atggcaatcg tcatgtccaa
     5521 ggatttgtta tggctatcca aaaatcagaa gaatcagcta atccatcttt gaaggcagtt
     5581 attcgtttgc tagaccttgc tccggtggtc aatgaagaat tactttcatt agctgattat
     5641 atgaaaaaaa ttacttacgc attcaaaatc acttgtctac aaacgatgct tccgagtgtg
     5701 atgaaagcag aatatgataa attgatctat cctttggcag acacaccaga agttcaggta
     5761 ttgtttgatc agcgagaagt gatttcctgg aaggaagcag aagaagaagg cagcctttct
     5821 caattgattc gctggcgcca agagcaatta gtagatatca aatatgaagt ccatacacga
     5881 aataaagtaa aaacgattcg tttggtccgt tctttgttaa ctgaaaagca aatcgaagaa
     5941 gaatgggcaa aattacggca aaatgccaag aaacaaaaag aacttctttt gtgtttatct
     6001 gaactgagcc aagaagaacc aattgcttat ttcaaaaata aaggaatcag tactgctgtc
     6061 ttgaatcaag gaaaagaaaa gggctggctg gagtttatag aatcagaacg ttatcgcgac
     6121 ccttataaag accgagtatt tgaccaaacg acggcattgg aactgaatgc agaacaaaaa
     6181 aatgctgtcg aacaaatcat cacagcaggt cagcagcaaa aagatcaggt ctttttgctg
     6241 gagggaatca ctggcagcgg gaaaacagaa gtatatcttc aggcaatcgc tgatgttttg
     6301 tcagaaaata aaacggctat tatgttagtg cctgaaatcg cattgactcc gcaaatggtc
     6361 gagcgattca aaggacgctt cggagaatca gttgcggtct tacatagcgg actttcccaa
     6421 ggtgaaaaat atgatgaatg gcgcaagatt gagcgggaag aagcgcaagt agtagtaggc
     6481 gcacgatctg ctatttttgc cccgctgaaa aatatcgggc tgatcattat cgatgaagaa
     6541 catgaatcaa gttacaaaca agatgagaca ccgcgttatc atgcgagaga tctagcaatt
     6601 tggcgcagta aatatcatca ttgcccaata gttctaggaa gcgcgacgcc ctctttagaa
     6661 tcaagagcac gagctcaaaa aggcgtttat cagttattgc agttaaacca ccgagccaaa
     6721 gcagctgcac agcttccagc tattgagatc gtagatatgc gtgaagagtt tcagaatcat
     6781 cggacctcta ctttttctgc taatttgcag gaaaaaatac aaaatcgatt agacaaaaaa
     6841 gaacaaactg tcctactgct caatcgtcga ggatattcgt cttttgtgat gtgtagggac
     6901 tgtggatttg ttcttccatg tcctaattgc gatatctctc ttacactgca tatggataca
     6961 cgttcaatgc gctgccatta ttgcggacat gaagaaccta ttcccaatcg ctgtcctaat
     7021 tgtggcggca ataaaatccg ctactatgga acaggtactc aaaaagtaga agaggaactc
     7081 cgagaacttt atccgcaagc taggattttg cgtatggatg tagataccac tcgaagaaaa
     7141 ggggcacacg aacagatttt acaaaaattc ggtgcaaaag aagcagatat attattaggg
     7201 acacaaatga ttgctaaagg tttggacttt ccagaagtga cattagttgg ggtgttgaat
     7261 gctgatacct ccctgaattt accggatttt cgttctagtg agcatacatt ccaattgctt
     7321 acccaagttt caggacgagc agggcgagcg gaaaaagcag gcgaagtcgt gatccaaaca
     7381 ttcaatcctc aacattacgc catagaattg gctaaaaaac aaaattatga gcagttttat
     7441 cagcaggaaa tgcatgtacg gcatcgtgga ggctatcctc cttattactt tactgtgaaa
     7501 ataaccgcca gccatcctga agaacaagta gcggcaaaga aaatctttca gattgccaat
     7561 cagctaaaag aagtgttaag ccctcaaagc ttgctattag ggccaacgcc cagcatgatt
     7621 ttgagagtga aaaaccgata ttattatcaa ctaatcatca aatataaaca tgaaccgaat
     7681 cttcctcatg ttttggatga aattttgaat ggaagccaga aagaacagcg acaaggtctt
     7741 tttgtggcaa ttgacaacga accattaaac tttatttaag aaagggccaa attatgcgct
     7801 atcctatatt gatccaccct aatgataaat taaaaagaac tgctcagcca atcgatgtaa
     7861 tcacggacga gacaatcgct ttattagaca atttatatga gacaatgatt gcgaatgacg
     7921 gaatagggat cgctgctcct caagtaggac aaaacaagag aattgctgtg atcgaagtag
     7981 atgaaggcga gaaatttgaa ctgattaacc cagaaatcat cgaagcaaaa ggtgaaagcc
     8041 ttgacgtaga aggttgtctc agtattccgc atgtctatgg aacggtcaag cgagcagatg
     8101 aagtgaccgt gcgttattat gatcgagacg gagaagagat agaagtgacg gcttttggct
     8161 atttagcccg tgccttccag catgaaatcg atcacttaga cggtatcttg ttcattgaga
     8221 aaatgataca gcagatcccg gaagaagaac ttgaagaata tatggaggaa catcttgatg
     8281 actaagctag tatttatggg cacacctgct ttttctgtgc cgattcttga agggctgata
     8341 gaatcaggtt acgagatcca ggcagtggta acacagcccg accgtccagt tgggcggaaa
     8401 cgagtaatta cgccgacacc ggtaaaaaaa gcggctctga agcacggtat ccgtgtgctg
     8461 cagccagaga aaatctccgg ttcgccagaa atggaagaaa tcattgaatt ggtgcctgat
     8521 gtgatcgtca ctgcggcatt tggccaattc ttgcctgaaa agttattgca agtaccaaaa
     8581 ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat atcggggagg tgcgcctgtt
     8641 cattatgcca tcatgaatgg ggagaaagaa actggtgtaa cgatcatgga aatgatcaaa
     8701 aaaatggatg caggcggaat ctatgcgcaa gaaagcatac caatcactaa acaagatgat
     8761 gtagggacga tgtttgaaaa gcttagcctt ttaggaagaa agctattgct agaaacattg
     8821 cccaatattt tggatggaca aaaaccagta cctcaagacg agtcagaagc aactttttcg
     8881 ccaaacatca cacgggaaca agaagcaatc gattggcata aaactgcaga agaaattgac
     8941 aaccaagtac gcggaatgcg tccttggcca attgcattca cgacctatga gggcatacgc
     9001 tggaaattat tatcggttga agtattggca gaaactacag acaaagcacc tggtacgatc
     9061 atccggaaag acaaaaaaaa tctatggatt gcttgcggta aacagaccgt cttagccatc
     9121 aatgaattac agccagctgg taaaggaaaa caggccgtac atgatttcct taacggaagc
     9181 ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa aaaaatccct aaaaaaatga
     9241 aaaaatcagt acgatatatg gcattactga cattagagcg agtgaacaag ggtggcgcct
     9301 actcgaatct tttgttgaat gagatgatca aacaaagcga gctaaatcaa aaagatgtgg
     9361 gtttatttac agaattgatc tatggtacga ttagccatca gcgactattg gaattttacg
     9421 ttgcaccatt gatcgctaaa gcaaaaaaag tggatgactg ggtcaagatg cttctttatc
     9481 tttctgtata ccaattagaa tttttagata aagtccctgc acatgctatt ttaaacgagg
     9541 cggtcgaaat cgcaaaagta aaaggaaatc ctgggacagg gaaatttgta aatggtgtct
     9601 tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg cattgctgat cctatcgaaa
     9661 gactttctgt caaaatcagc ctgcctctat ggatgacgaa acgactggtg gatcaaatcg
     9721 gatacgaaga aactgaaaag ctcggtttat cgctatatca gcctagccat gctagtgccc
     9781 gagtagatac acgccgactt tccagagatc aggccatcac tgttttgcaa gaagaagaga
     9841 tcacagctga aaaaagccaa gtcagtccat acggtatagt agcgaaaaaa gggcatttag
     9901 cttctagctc attgttccac gatggcgtca tgacgatcca agatgaaagt tcgatgcttg
     9961 tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt agatgcctgt gcagcacccg
    10021 gaggtaagac gacgcatatt gcttcttttt tagatccgtc atgcggtgga gaagtggtcg
    10081 ctttagatgt acacgaacac aaagtcaaac tcattgaaga taatgcagca cgtctaggtg
    10141 tctcttctgc tgtctacgct caaaagatgg atgctagaga agtcagtaaa cagttcgaag
    10201 cagagcagtt tgatcggatt ttagtagacg cgccttgttc aggtcttggg ctgcttcggc
    10261 gaaaaccaga tattcgttat aaaaaacagc cggatgaatt agcgaattta ccaaaaatcc
    10321 aattggaaat tttagaaagc tgcgcaccaa ctttaaagtc ttccggtatt ctgacttata
    10381 gcacttgtac gatcttaaaa gaagaaaacc aagacgttgt ttctgctttc ttggaaagac
    10441 accctgattt tgagaggatt gatgttttga ccaatcctat gctacaatca tcaatcgaag
    10501 agaaaatgtt gactttgtat ccgcatcagt tttacacaga tggatttttc atttgttgtt
    10561 tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata tcaatcagat gtaggaagac
    10621 gacgtaacac aaatcaagac tatgccagtg tatttaccag ccgttcaggt ctcaagcttg
    10681 ctgtattagc agacgggatg ggtggtcatc gtgccggaga cattgccagt cagatggcgg
    10741 taacgaatct aggcgaagct tgggaaaaac aagaagctgt tgacgatgaa aaaatcgccc
    10801 aatggttcat tcaatcgatt caagaagaaa atatgctgat ttatcagcgt ggacaagaac
    10861 agccggaata taacggtatg ggaacaacaa tcgtagcggc cgctctgtca gatgactggt
    10921 tcacgattgc caatgttggg gatagcagag cttatttgat tcgtgaagat aaaatcatgc
    10981 aattgacgga ggatcattct ttagtcaatg aactagtcaa atcaggagaa atcagcgaag
    11041 aaatggcagt gaaccatcca aggaaaaata ttttaacacg ttctgtcggc atgcctggaa
    11101 cagtcgaagt agatgtttct actcatcttt ggcaactaaa agatcgactg ctgttatgtt
    11161 cagatggctt gacgaacatg ctgtctgaag aagaaatagg aacgatcatc aatcaagaag
    11221 gcccattgtc tcataaagta actgagctga ttgatcgtgc caatgaagca ggcggagcgg
    11281 ataatatcac cgttcttctt atcgaatata aggaggacag tgtatgatcg agttgggcaa
    11341 aaaattaaat ggccgttatc aaatcactgg aaatatcggt agcggtggta tggcaaatgt
    11401 atttttggcg cacgatttaa tcttggatcg tgatgtagca gttaaagtct tacgatttga
    11461 ctttcaaaat gaccagactg cgattcgacg atttcagaga gaagctttag cagcaacaga
    11521 actcgttcat ccaaacattg ttagtgttta cgatgttggt gaagaagaca acatgcagta
    11581 tctggtgatg gaatacgtca aaggaatgga cttgaaacga tatatccaaa cacattatcc
    11641 tgttccttat gaaacagcag taaatattat gcagcagatc ctttctgcta tttctttagc
    11701 tcataaccat caaatcatcc accgagattt gaaaccccaa aacgtactga tcgataatga
    11761 aggtgttgta aaaatcacgg atttcgggat tgctatcgct ttatctgaaa catcgatcac
    11821 gcagacgaat acgatgctag gatctgttca ttatctgtcc cctgagcagg ccagaggaag
    11881 tatggcaacg aagcagtcag atatttatgc tttagggatc atcctttatg agatgttgac
    11941 gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt gctttaaaac attttcaaga
    12001 tgatcttcca tctataaaag cacttgatcc aaatgtgcca caagcactgg aaaacgtgat
    12061 tttacgggca acggctaaag aacctgcaga ccgttataag tcagccgagg aaatgtctga
    12121 tgatctttct acagtattat caccagctcg tgcaaacgaa gaaaaatggc agcctcatgt
    12181 gatggataat gaaacaaaag ttatcacacc ccttacggaa gatgcaccaa tgcctgattc
    12241 attcaaatct atgcctctgc caaaagacaa gccagaaaac gaaaccttac cagaggagga
    12301 agaagttctg cctgatccta aaaagaaagg acagaaaaaa tggtggctga tcctattagc
    12361 tattcttgct gttttaggaa tcggaacagc tgtattcttt gcttctggcg ggcgaggaga
    12421 agttgttgtt cctgatgttt ctgatctttc tgaagcttct gctagagaaa cactcgcaaa
    12481 agcgaaatta gaagtagcaa caaaaacaga agaaattgca gatgacaaaa tcgaagaagg
    12541 taatgtagtg aagacagacc cggcagcggg aacgacagtc aagcaaaaac gcgaagtcac
    12601 tctgtatatt agtagcggca cgaaaaaaat caaattagat gattacagtg gtaaaagtta
    12661 tgaagatgcc gaagcaagtt tgaaagaatt agggttttct gctgacttga ttcaaaaaga
    12721 agaggaattc agttcagagg tagaaagcgg aatgatcatt tcgcaatctc catcagaaga
    12781 tacagaagtc gatcctaaaa cagacacgat tactttcaca gtcagcaaag gaccagaaac
    12841 agtgactttg agtagtcatg taggagaaaa ctacgaaact gttcgtaatt ggcttgttac
    12901 acaaggattc aatatcaata atattagtgc tacttatgac tatagcgaca ctgtaccatc
    12961 aggttcgatc atcagtcagg atccagcctc gggtgaagtt gtagcagaag aaacttacat
    13021 caattttgtc gtaagccaag gaacagagcc taaattgaat gatatctccg gctatacaaa
    13081 aagcgaagca caaagttatt tagctagtgt cggtgcagaa tatatcgggc atgagacttc
    13141 cgaatattca aacacggtgg ataaagataa agtgattcgt acagtaccag gggcaggaac
    13201 gacaattacc aaaggaatgg ttgtgaacgt aatttactct aaaggaccag atccagcgtc
    13261 ttctagttct gaaaaagact cttcagacag ttctagcagc tcaagtagct ccagtagcac
    13321 aagcaactct tcaaaagaag aatcttccag ctccacaagt tcatcagcta gcacctcaga
    13381 aagttcaaaa taagtgctga ataaaatata tcagaaggga atgcgacatt gatttgttgc
    13441 attctctttt ttgtgtttct atctcttttg atcaattttc aagccatata ctttgagaag
    13501 tttgcatgga ataacaccta ctttcctctt cgtatggtaa aataaacaaa aacggaggtg
    13561 gcacttatca aaggacaaat cagaaaagca atcagcggtt tttactatgt atacgcaaac
    13621 ggagagacct atcaaacaag aggaagagga aatttccgga atcggaaaat cacgccgctt
    13681 gtcggagatc aggtgatctt tgagagtgaa aatcaaacag acggctatct tctggaaatc
    13741 ttgccgagaa aaaatcaatt agttcgccca ccagtcacaa atgttgatct tggaatcatt
    13801 gtcacaagta tcgtagagcc tgcattttct tataatctac ttgaccgctt tctagttacc
    13861 ttggaatacg agtcgatcga accgattatt tttttgacaa agaccgactt gtcagacgac
    13921 ccagaaaaaa tcgaagagat caagcaaacc tatactaaaa taggctatcc ggtgattgta
    13981 cccaatcata cgggtgatac gacagaactg atccgttact tcccagaacg gctatcggtc
    14041 tttatgggtc agtctggagc aggaaaatcg acgctactga atcaaatatc accggaactg
    14101 aatttagcaa caggagagat ttctgatgca cttggaagag gacggcatac aacacgtcat
    14161 gtggaactga ttcctatcta tggcgggtta gtagcagata caccaggatt tagctcgatt
    14221 gactttttga caattgaagc aggagaactg ccgagacaat ttcctgaatt tgtagaagct
    14281 tctgtccatt gcaaatttag agagtgcatg catgctaaag aacctggctg cgaagtaaaa
    14341 catagagtag aaacaggtgg tatcgcacag acacgttacg acaattattt gcaattctta
    14401 caagaaatcg aaaaaagaaa accaatgtat gcaaaaaaag aaggtaaacg aaaataggag
    14461 gaaacaagaa tgaagatagc accatccatt ttgagtgcag attttgccaa tttacaaaga
    14521 gacattgagt tagtggaaaa aggaggcgca gattacatcc acgtagatgt aatggatggc
    14581 cgatttgtcc caaatatcac attagggcca aatattgttc aggcaatccg tcctgttact
    14641 aaattaccgt tagacgtcca tctgatgatc gttgatccag aaaactacat tccagcattt
    14701 gcaaaagctg gagcagatat cattactgtc cacgtcgaag caactcctca tattcatcgg
    14761 gcacttcaaa tgacaaaaag cttaggagtt aaagctggag tagttatcaa tccaggaaca
    14821 ccagtctcca tgatcaaaca tgttttgcct ttggctgatc aagtactagt catgactgtc
    14881 aatcctgggt tcggcggaca aagctttatt gaagaaacag tagaaaaaat tacggaactc
    14941 gctgatctac gtagagagaa caattggact tatgcaatcg aagtagatgg cggaatcgtt
    15001 ccagaaacag caaaaatctg taaagatgca ggggcggatg tcttcgttgc aggatcatat
    15061 atctatggag ccgaaaatcc agtcggacaa atcgaacgat taaaagaggc attagactaa
    15121 atgaaaattt tattagctgc tggaggtaat ccgcaaaact ggccgcagtc tctgctgtcc
    15181 aaatacgatg cctatgtcgg gatagaccgt ggtgcgctgt acctgcttca agcaggctac
    15241 ccagtagatc tggcagtggg tgattttgat tcattgtcga acgaagagca gaaggaagta
    15301 tttgcaaaag ccaaagagta tacccgttcc cctgcagaaa aagacgatac agatacacag
    15361 ttaggtatct taaaaacatt tgaacgttac ccgcaagcgg aagtcacttt gattggagct
    15421 acgggcggaa gattggatca tcttttagcc aatctctggt taggtatgga acctcgcttc
    15481 aaaccctttg cccatcagct tattatcaga gattgtcaaa attatttgtc ttattatttg
    15541 cctggagagc atatagtaag acagatacca gatatgaagt atttagctta ttgctgcttg
    15601 actcccgtct cgaatcttac attaaaaggc agcaagtatg tattggaaaa taaagaagta
    15661 gaatatccga catcttatgc aagtaacgaa tttctttcag ataccgcaga attttctttt
    15721 tcagatggta tgattgcagt tattcaaagc aaagattgat ccataaacaa ataaaaaaat
    15781 ttgtctttca ggctaaaaat gacagaatct attatttgct aaaaaaaagc ttaagaggag
    15841 caacttacct tttgtttcac ggcatttagt gataaaattg aacacaagag tctaattaat
    15901 tcgaggtgtc tttgtgaaga agttcaatcc taataaaaat attattatta ccttggttat
    15961 tgttatcatc gtggtgacga tactaagtgt gaccgctgcg cagcgagcga atgaaggaaa
    16021 gcccaatttc ttccaatcca tcgttaatga cagtgttgca atggttgata aaacaatctc
    16081 tgccccagta cggtgggtac gaaatggagt agattctgtc cataatcttt tttcaactta
    16141 tgccgaaaat gagcggctaa aagaaaaaat cgatagctat gatgacgttt cacagcaaaa
    16201 caagaattac gagagagaaa tcgaagcgtt gaaaaaagaa cttgagttga atcagacgct
    16261 gactagttac gaaaaagtaa cggcaaatgt cattactcgt tcgcctgaca gttggcagga
    16321 catgctgatc gtagacaagg gttctaaaga cggtatcgaa gcaaatatgg cagtcatgtc
    16381 gcaaaaagga ttgattggcc gagtgatcga ggtcaatacg gcttcgtcta aaatcgaatt
    16441 actgtcatcc tctaatgaaa gctccaatca ttttccagta cgggtatctt cggctaatgg
    16501 cgaagcgttt ggtttgctta aaaactatga tgaaaagctc catgccttag tggtgaccca
    16561 attaactggt gatacggata tcaaagaagg ggatgttgtc cagacatccg gtcttggagg
    16621 gaattctcca gctaacttgc cgatcggtac ggttattaaa acgaaaccag atagttatgg
    16681 gctggatcgg gaagtttatg tgaaacctta tgcagaaatg tatgacgtgt cagttgtgac
    16741 gattgtccaa cggctagcgg aggattagtc catgttgaag aaagaaacaa tgaagtacta
    16801 tctgccaatc gttttgttct ttttgatgtt gatagatggt catttaacaa gaatgctagg
    16861 ggagtggtcg aaaggcacct atatgtcaaa tgcccacttt ctgatattgg cattattatg
    16921 ttgcagtatg gcgtttgaaa aacgttattt actgattacc acgattgttc tcggggctat
    16981 ctatgacgct tactatattg gcgttatcgg tatctatgca gtagctctcc ctttaattgt
    17041 atggttgatg tatgtaatga aagacgttat ccatgtcaac atctttactg aatttttcag
    17101 tatgatcatc tttgtcacgg gttatgaatt gtttacgatg gtggtccagt tgatttttaa
    17161 attagcagta gtaaataaca cgtattttat tacaaggttt ttaggaccta cactgctgtt
    17221 gaacatgatt atatttgtat tattcatttt tccctttaag aaattattca gtgatgaata
    17281 atttcttttt ttatttttag gtcgaaagcg aaaaagaatc gttgttatct taagaaatgc
    17341 tagacttaac gcggcttttt catgatcatt ttctgaaacg aacagttttg tcatttttaa
    17401 aagttgtttt gaaacatttt tgtaacataa ctattatatc gttgacacac tgctatggta
    17461 caatttttat atcgttaaga aagacttaaa tatatttaga tatttattaa attaagccag
    17521 tcggaggaat gaagagtgaa aaagagttta atatcagcag taatggtatg ttcaatgacc
    17581 ttgactgccg tagcatcacc gatcgctgcc gcggcggacg attttgattc tcagatacaa
    17641 cagcaagatc aaaagattgc agacttgaaa aatcaacaag cagatgcaca atcacaaatc
    17701 gatgccttag aaagccaagt ttctgaaatc aacacacaag cacaagacct attagcaaaa
    17761 caagatacat tgcgtcaaga atctgcacaa ttagtaaaag acattgcaga tttacaagaa
    17821 cgtattgaaa aacgtgaaga tacaatccaa aaacaagcac gcgaagcaca agtaagcaac
    17881 acaagctcaa actacatcga tgctgtattg aatgctgatt cattagcaga tgctattgga
    17941 cgcgttcaag caatgacaac gatggtaaaa gcaaacaacg acttgatgga acaacaaaaa
    18001 caagataaaa aagctgtaga agacaaaaaa gctgaaaacg atgctaaact aaaagaatta
    18061 gctgaaaacc aagctgcttt ggaatctcaa aaaggcgatc ttttatctaa acaagctgat
    18121 ttgaacgtac tgaaaacttc attagctgct gaacaagcaa ctgctgaaga caagaaagct
    18181 gacttgaacc gtcaaaaagc agaagctgaa gcagagcaag ctcgtattcg tgaacaacaa
    18241 cgtctagcag aacaagctcg tcaacaagct gcacaagaaa aagctgaaaa agaagcacgc
    18301 gaacaagcag aggctgaagc acaagctact caagcttcat ctactgcaca aagctcagca
    18361 acagaagaat catcagctac acaatcttca atgactgaag aatcatcatc agctacacaa
    18421 tcttcagcaa cagaagaatc aacaacacct gaatcttcaa cagaagaatc aacagcgcct
    18481 gaatcttcag caacagaaga atcaacaaca gtgcctgaat cttcagcaac agaagaatca
    18541 acaacagtgc ctgaatcttc aacaacagaa gaatcaacaa cacctgcgcc tacaacacca
    18601 tcaacagatc aaagtgttga tacaggaaac ggcacaggaa gtagtactcc ggctccaacg
    18661 ccaacaccaa cacctgaaca accaaaacct gtaacacctg ctccagctcc ttctggaagt
    18721 gtgaatggtg cagcaatcgt agcagaagct tacaaatata ttggtactcc atatgtttgg
    18781 ggcggtaaag atccaagtgg ctttgactgc tcagggttca cacgctatgt ttacatgcaa
    18841 gtaactggtc gtgacatcgg tggttggaca gttcctcaag aatcagctgg aacaaaaatt
    18901 tcagtttctc aagcaaaagc tggagacttg ttattctggg ggtcacaagg cggaacttac
    18961 cacgtagcga ttgctttagg cggaggacaa tatatccatg ctcctcaacc aggcgaaagc
    19021 gtaaaagttg gttcagttca atggtttgcc cctgactttg ctgtcagcat gtaagaatga
    19081 ttaatcaaaa agagttagcc cattctggac taactctttt tttattgtga taaaatagaa
    19141 atcagataaa ttcttctgtc aattccatga acgtttggat atcttcagtc accatgtcga
    19201 tacctgcttg ccaaaaatct ggttgggtaa gatcgacatt caaatgtttc ttcgcaagat
    19261 cttccgtcgt cattgaggca gtatcgcgta gtaaatggat atattcttct tcaaaatttg
    19321 ctcccttttg gttggcgtaa gcatagatgc ccaaactaaa gaggtagcca aatgtgtatg
    19381 ggaagttata aaacggtacg tcatcaataa agaaatgcaa ttttgcagcc cagaaatatg
    19441 gatggtaagt agctaaagct ccttgatagc cttctttttg cgcctcaacc atcatctctg
    19501 tgatcttatc ttctgagact aatccttctt gccgtgcttc atagaatcga ttttcaaaaa
    19561 tgaatcggga atggatattc atgaacatag ctaaagcatt ttgcagtttt gtatctagca
    19621 aattgatttt ttcttctttt gtttttgcga cttttaatgt agcatcggct acgatcagtt
    19681 ctgcgaatgt acttgctgtt tctgcgacat tcatggcata ttcgcgattt aaagaaggta
    19741 aatcccacat cgtgctgcta tggaaagcat gtcctagttc atgagcaagt gtcgcaacct
    19801 cattaacaga attactgtag gtcataaaga tccttgattc ttttgtttct ggtaactcag
    19861 tacaataacc accaggacgt ttcccaggac gatcttctgc ttcgatccag ctcttttcaa
    19921 aagcagattg ggcaaattta gccatttttg gactgaattt gttaaagttt tcgataataa
    19981 atgctgctgc ttcgtcaaaa ctaaaggttt tttctttcaa atcgcctaaa ataataggtg
    20041 catcttgatc ttgccattcc attttttctt taccgaaaag attagctttt ctagtcaagt
    20101 aattaacaag gggttgtttg tttttctgga ttgtatccca catgacagac aatgtttctt
    20161 tcttcaaccg attgtactct aatggttttt gtagaaaatc tgtgacacca tgaagttcat
    20221 agtcagaaag tctaaaacca tctaaatgat tcaaagtatc cgtgaagagg ggtgcctttt
    20281 ctttccaggc ttcttcccat gctgcgaaaa gtgtttctct gacttttgga tctggatcgc
    20341 ccatcatctt attgaaggct tgtccagcag ataattcgac tacttgtcca tcttgttcga
    20401 agggaatcga aatgctggct acaatcgtat cataatgact gctccaagca tttagaccat
    20461 ctaaagaaag cgtatttata atgttttctt cagcttctga taataattgt gagccatcac
    20521 gacgaatctc gtttaaacga aaagcaattg tttcaaacga agattgagaa agcatctgat
    20581 tccaatcatt gtcagatatt tctgcaaatt tctttgacaa tacagtttcg gctgattgca
    20641 gacgaggcag cattgcgtaa agcttgcctg ataagacctt tgcatctgaa tcattgacat
    20701 tggcagaaag caatgcagta atatagctgc tgcattgggt aaaaccattc gtaatcgttt
    20761 cttgcaattg aagaatagcg tttaactgct ctgcattgtc agaactaggt gaccattttg
    20821 ttacgcggtg ataatactcg ttcatttggt cttccaattg cttcatccgt tggttcaaag
    20881 catcagaatg actgcctccg gaaaaaatag aatctagatt ccaatttaat gaataactca
    20941 tgaaaaaact cccttcatat tgtaatacaa aattaagtat agcacttttt tatggaagaa
    21001 gtctctatga aaatgattgt tgtttcttca aaaaaagtct aagataaaat acagaggtga
    21061 cttatgaatt cttcaaaaaa acaaatgaaa aacggaaata tttatttagt aatcgcactg
    21121 atagtcgtta taatcttatt ttacagttct tctcaaactt acggacagca atcacaagtt
    21181 tcgcgattag aaagctggct gcctaatcag ccatttaaag acacattagc gggtatacgt
    21241 tttaattatg gtggaagtat ggtgagtatc aaccatctgg gctacgccaa atttttagag
    21301 ttctttttaa ggaagggcgc acatttcggt acctatttta ttttgggagg cagcttgttt
    21361 ttaggtgttt tccccaagct aaaaatttgg tggctgacag cgatccttgc ttggctttca
    21421 gcaacagggt acgcaggttt ggacgaattc catcagatga tgaccggtgg acgaacacct
    21481 atgttccaag atgtcatgtt ggattcgatc ggtgctctga ctgctgtgat attatgtgtg
    21541 ctttttgcgg cattcaagaa gcgtttatag attggctgtg gctttgtttt tttgtgaaat
    21601 agtactagaa tagaagtgta ttctcaaaag aacaagggag gaaaaatcat gtcaggacat
    21661 agtaaatggc ataatatcca aggaagaaaa aatgcccaag atgcaaaacg agggaaagtc
    21721 tttcagaaac tttctcgtga aatttatatg gcagctaaag caggcggtcc tgatccatct
    21781 atgaatccag ctttgcggct tgttgtggac aaagcaaaag ctgcaaatat gcctaacgac
    21841 aatgtggaac gggcgattaa aaaggctacg agtgccgtag atgaaacgaa ttacgatgaa
    21901 atcacgtatg aaggatatgg tccgggtgga gtaggaatct tagtccatgc attgacagat
    21961 aaccgtaatc ggacagcgac aaatgtccga gtagctttta ctcgaaacgg tggatcactg
    22021 ggagaaacgg gttcagtcag ttacttgttt gaccgcaaag gatatatcgc tattgaacgc
    22081 gatgggttga ctgttgatga agagacgatg tttgaggatg tattagaggc gggggcagaa
    22141 gatctgcaaa cttctcctga agtattcgag atttatactg caccagaaga ttttgctgct
    22201 gtacgagatg agttggaaaa agagttcact ttggcgcaag cagaattgac gatgattcca
    22261 caaacgacga ttgatttaaa tgaggaacaa aaagagcaat tggaacgtct agtagataag
    22321 ctagaagacg atgatgatgt ttcagaagtc tttactgctg ctgaaatgta agtagcacat
    22381 tgttagataa taaaaagaaa gcacctgtac caatcgtacg ggtgctttct ttttatatat
    22441 tatttttgtt agaagaccaa tacaggcgtt cctttttcga ccatcccgaa taattccttc
    22501 attacacttg gtggggtgtt gatgcagccg tgtgaaccgc gggttttcca taagtctcca
    22561 ccgtattcag gttgccagtc tgaatcgtgg ataccaacac ctgtccagtc aatcggcatc
    22621 cagtagttta ccggactctc gtaaggtgtt ccatcgtcat ttgttccttt taaagttgca
    22681 tcttcttctt tattccaaac atagaagact ccagcaggtg taggtgtagt tggctttccg
    22741 gaaacgatat ctgtttcaag ggccactttt ccgtctttgt aataccacat atgctggttc
    22801 tccagatcaa cttcgatata cgtatcttcg atcaatggat gatcagcggt tgtgccacct
    22861 tgcacaatgg gtgaacgagt aaaatcttgg cctgccagaa tcgctttttt caatgcttct
    22921 gtctcactgt ctgtttggat cgtccaactg taagtgccaa ctgggaccgt aacttctcct
    22981 ctttttgtac ttttaaattt tgtgtcattc gtacttgtgt tatactttgt gcctaaatct
    23041 gttacatatt gacgtacttg ttctgtatct aaatcgactt ttccatcatt gtaggtcagc
    23101 caactcataa tatcacttga tgggatctgg aaagtctcac cgtttatact gtaagtagct
    23161 tttacatttg cgatagcatt catggatgcg agttgttctt tcagtgagct gtcttcagat
    23221 gttacttttg gtttttcttt gaattcagtc aattcaattg tatctttacc agagtttacc
    23281 gcacttttta attgtttgac cgcagcatcg acgtcgatcg tatttccatt gacttcaggc
    23341 ttgataacga aagagtcacc tgctttttcg atcgttgcat cagtagtagg tgtccggtct
    23401 ttgttcaaat cagtcagctt agttgtcaat gtctgaacgg tagtgtctag tttttggtgg
    23461 ttttgactga caggatctat ttcttgtttt tctgccgctg agacataagt cattccccaa
    23521 gtccatccgt tttgttcatt taggatcgga gaaagctccg aagtgaaatc atctttgtag
    23581 ccgagctcgg attttggtac ttcctgccag acttttccat cttctttaat agccagtttt
    23641 ttatcagaat aagcttcttt taatttttca tttgcttttt cgactgttag attgctgaca
    23701 tttattccat ttatttctgt attcggcaaa aaatgctttg tgtagtatgt actgcgatag
    23761 gtataaaagc tgatcagcac taagagaaca ccaagaactg aaagcaaagc aacatgtttc
    23821 gatttttttc gatgcgaaga tcttgtcata tcgacaactc cttaagtaaa agtttagggt
    23881 ggaacggtct catgatagca taatttagag gaaaagacga aggttatctc tactttatga
    23941 aaaaatttag aaaccataag ggtgataaaa taaaaaaacg gaaaacaccg aatagatgtt
    24001 ttccgtcatg ttcaaagatt attcagcgtc tagtttagca aaagcttcat ctagaatttc
    24061 ttttaattgc ttagcagaag cagccatacg gtcttgctcg ctgtcactta aaggaatttc
    24121 gattactttt tggatacctt gacggttgat aacagcaggt gcaccaatgt agatgtcgtt
    24181 caatccatat tcgccattca tgaatactga taaaggaaga acagcatttt catcatctaa
    24241 gatggcacga gtgatacgtg ctaatgctac tgcgatacca tagaaagtag cgcctttttt
    24301 ctcgataatt gtgtaagcag cgtcacgtac gccaaagaat aggttaacca tagcttcttc
    24361 atcgatttct gggttgtttt ttacccattc atagatttgc aagccggcaa catttgcatg
    24421 tgaccaaact gggaattctg aatctccgtg ttcacctaag atgtaagcat ggacgttacg
    24481 tgcatcaacg tcaactaatt ccgcaagtgc ttgacggaaa cgagctgagt caagagaggt
    24541 acctgaaccg attacgcgtt ctttagggaa acctgagaat ttccaagttg agtaagtcaa
    24601 aatatctact gggttagcgg caactaaaaa gataccgtta aatcctgatt caacgatttg
    24661 tgtgacaaca tcgcggttga tttttaggtt tttgtttact aagtctaaac gagtttcacc
    24721 tggtttttgt ggtgcgccag ctgtaataac aactaagtca gcatcatgtg cgtctgcgta
    24781 agtagcagaa tagatttttt taggtgaagt aaatgccaaa gcgtgagaca agtcaattgc
    24841 gtctccttca gttttagcag tattgatatc cacaatacct acttcttgag cgatattttg
    24901 agtaactaga gcaaaggcat agctagaacc tacggcgccg tctccaacaa gaataacttt
    24961 ttgatgatct tttttttctg cagctgcagt catttagtat caatccttcc attgatttgt
    25021 tttttcccat gcttatgcta acatttctca agccccatgt cacgctattt ggctataaaa
    25081 tataacagat aacaaggttg tattatatgt aaaag
//
LOCUS       DAHUWM010000017        24122 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHUWM010000017 DAHUWM010000000
VERSION     DAHUWM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.017"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            173..742
                     /locus_tag="NK788_000488"
     CDS             173..742
                     /locus_tag="NK788_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6768840.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(920..1360)
                     /locus_tag="NK788_000489"
     CDS             complement(920..1360)
                     /locus_tag="NK788_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCH6768841.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1518..2282
                     /gene="map"
                     /locus_tag="NK788_000490"
     CDS             1518..2282
                     /gene="map"
                     /locus_tag="NK788_000490"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCH6768842.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2314..3237
                     /locus_tag="NK788_000491"
     CDS             2314..3237
                     /locus_tag="NK788_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCH6768843.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3313..4452
                     /locus_tag="NK788_000492"
     CDS             3313..4452
                     /locus_tag="NK788_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCH6768844.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4445..5245
                     /locus_tag="NK788_000493"
     CDS             4445..5245
                     /locus_tag="NK788_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6768845.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5245..6072
                     /locus_tag="NK788_000494"
     CDS             5245..6072
                     /locus_tag="NK788_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6768846.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSRNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            6050..6784
                     /locus_tag="NK788_000495"
     CDS             6050..6784
                     /locus_tag="NK788_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6768847.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6884..7750
                     /gene="rfbA"
                     /locus_tag="NK788_000496"
     CDS             6884..7750
                     /gene="rfbA"
                     /locus_tag="NK788_000496"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCH6768848.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7764..8336
                     /gene="rfbC"
                     /locus_tag="NK788_000497"
     CDS             7764..8336
                     /gene="rfbC"
                     /locus_tag="NK788_000497"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCH6768849.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8358..9386
                     /gene="rfbB"
                     /locus_tag="NK788_000498"
     CDS             8358..9386
                     /gene="rfbB"
                     /locus_tag="NK788_000498"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCH6768850.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9484..10335
                     /gene="rfbD"
                     /locus_tag="NK788_000499"
     CDS             9484..10335
                     /gene="rfbD"
                     /locus_tag="NK788_000499"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCH6768851.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10370..12403
                     /locus_tag="NK788_000500"
     CDS             10370..12403
                     /locus_tag="NK788_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768852.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12447..13727
                     /locus_tag="NK788_000501"
     CDS             12447..13727
                     /locus_tag="NK788_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCH6768853.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13937..14743
                     /locus_tag="NK788_000502"
     CDS             13937..14743
                     /locus_tag="NK788_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6768854.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14755..15975
                     /locus_tag="NK788_000503"
     CDS             14755..15975
                     /locus_tag="NK788_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6768855.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15965..17551
                     /locus_tag="NK788_000504"
     CDS             15965..17551
                     /locus_tag="NK788_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6768856.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17590..19728
                     /locus_tag="NK788_000505"
     CDS             17590..19728
                     /locus_tag="NK788_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6768857.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(20096..21079)
                     /locus_tag="NK788_000506"
     CDS             complement(20096..21079)
                     /locus_tag="NK788_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCH6768858.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21457..22848
                     /locus_tag="NK788_000507"
     CDS             21457..22848
                     /locus_tag="NK788_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCH6768859.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22864..23904
                     /locus_tag="NK788_000508"
     CDS             22864..23904
                     /locus_tag="NK788_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6768860.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23923..>24122
                     /locus_tag="NK788_000509"
     CDS             23923..>24122
                     /locus_tag="NK788_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCH6768861.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPK"
BASE COUNT         7720 a         4012 c         4950 g         7440 t
ORIGIN      
        1 aataaaaaaa ggaggttatt tttttaaggt taagtttcat gatatatgtt actacttgct
       61 atttcagctt gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac atgttacaat
      121 gaaattaatc aatggtggaa gcgttataaa aaaattaagg agattaaaaa atatggcaag
      181 aaaaaagacg attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga
      241 tggatttaca cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc
      301 gatttatctt gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta
      361 tgatcattta caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct
      421 tgcttttaat tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt
      481 agaagaatat ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac
      541 agtgaaacgc gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa
      601 tgggacatgg atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc
      661 aactgatgaa cagattgaaa agatgatcca agacagtata gatgctatct tgaaattcaa
      721 agaaccaatc gaaatcgatt gaatatactc aaaagttcca caaatcagta ggtaacaaga
      781 gatgtgtcaa aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga
      841 agaactcttc catgaatgca atgaataaaa gagccataca gattgaataa atctggatgg
      901 ctcttttatt gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat
      961 tttcgatatc ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg
     1021 cgcctgtttt tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa
     1081 aagtatcacc agatccgcag acaccgtaaa tttttcccaa tagatccaat tcttgcaagt
     1141 cttcataaaa gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta
     1201 ctacgcaaat atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt
     1261 cgacttcgat atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt
     1321 ttcctgtcat gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata
     1381 attatttaac aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga
     1441 cacctgggga atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt
     1501 aaaaaggaga cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag
     1561 aatctggagc tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa
     1621 tcacaagttg ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg
     1681 ctgcgcaaat tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa
     1741 tttgtcatgg attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata
     1801 tgtgtatcga tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat
     1861 ctacacctga gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg
     1921 agcaagcaca agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag
     1981 aaggcgaaaa tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc
     2041 atgaagaacc agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag
     2101 gcatggttat cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc
     2161 ctaacggctg gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat
     2221 tggctattac taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt
     2281 gatctatcca gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa
     2341 aatgaaaatc tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg
     2401 agtacgacct ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt
     2461 gcttttggta atattttacc gtttctccac atcgatcaag agactgtact gacctattta
     2521 cgagaagtga ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg
     2581 acacaaagtt caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt
     2641 caaagtatca atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa
     2701 aattttttga tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt
     2761 agtggggtaa ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc
     2821 gtacaaatac ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg
     2881 gtagctttat ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag
     2941 ttgaagacga tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa
     3001 gtgtttagta tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga
     3061 agttttattg tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga
     3121 atcatcaatg cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat
     3181 ccaagccgaa aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa
     3241 caagagttct tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa
     3301 gtaggtgccc atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta
     3361 ttatcactga tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac
     3421 aaaccaaatg aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc
     3481 tttatttcat ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat
     3541 attgtaccga tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa
     3601 tatgagctga cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat
     3661 tttgtagcag atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta
     3721 ggctggctta gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat
     3781 ctgattgacg gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata
     3841 ggaatcattg gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc
     3901 tgtcttgtag caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat
     3961 ttaggcgata caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg
     4021 ctgaagaacg tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta
     4081 acagacacgt tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca
     4141 gataaaaaac atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc
     4201 ctgacgattt atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact
     4261 ggaagaatcg gaacgatcgt attgattatt gccatgttgt ttgctgcaat tttattattt
     4321 gaattgattg gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta
     4381 gggaataaag agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat
     4441 atagatgaat aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga
     4501 taaaattttt cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa
     4561 aatccgaatt tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag
     4621 gaaatatccc tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca
     4681 taatcacaat ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt
     4741 agtggatgaa gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc
     4801 gatgttagca cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca
     4861 gctggctgtt tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt
     4921 tgacaaacga ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt
     4981 acggatgatc tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg
     5041 gtttgatgag cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa
     5101 agcaggttac aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg
     5161 tgcgcatcga aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa
     5221 taagtggggg tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat
     5281 cagcaactat tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag
     5341 caaattcagc ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa
     5401 aaagaaaata tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat
     5461 tttgcgttgt ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg
     5521 aggctagtcg acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt
     5581 gctgcagttc cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt
     5641 tatatcaatc gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg
     5701 gcaatcaatt caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat
     5761 cctgcttttt ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa
     5821 aaaactgtgg agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa
     5881 aaagtttcaa ggaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa
     5941 tatgatcaaa ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag
     6001 ttcttgaccg tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat
     6061 tggaaggtgt gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt
     6121 aagagagcag ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc
     6181 tgatgatggg tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa
     6241 gcgaatccag cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat
     6301 ccagcagacg aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac
     6361 aaaagtgaaa aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc
     6421 tgatttaatc attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg
     6481 aaattccaaa gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat
     6541 ggctttccgg tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca
     6601 tgatatgtgg attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac
     6661 tctgtaccga agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca
     6721 aaaaatttct tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg
     6781 ttgataaaag atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata
     6841 ataacaaagt gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct
     6901 tgctgggggc agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat
     6961 gccgatttat gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat
     7021 caaagaaatt ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg
     7081 aaacggaaat gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt
     7141 ggcacaagcg tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt
     7201 aggggacaat atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa
     7261 agatacaggc gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt
     7321 agaatttgat gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc
     7381 aaattacgct gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg
     7441 tatcaaacct tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga
     7501 taaaggaaaa ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac
     7561 acatgaatca ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt
     7621 aaaagtggct tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt
     7681 gctggccctt gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc
     7741 cgctgaataa ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa
     7801 tcgtagaaat ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag
     7861 caaaatttgc agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag
     7921 ctgaagctgg tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat
     7981 tgattcgggt agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc
     8041 cgacttatgg tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg
     8101 tacctaaagg atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata
     8161 agtgtgataa ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt
     8221 tagcgattga ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc
     8281 atccgacatt aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat
     8341 taaggagaaa ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct
     8401 ctaatttcgt tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata
     8461 aactgactta tgcaggaaac aaagagaact tggccggact tcctagcgat cgcgtagaat
     8521 tggtcgtagg agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg
     8581 cagttgttca ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat
     8641 ttgtacaaac gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg
     8701 tacgctatca tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc
     8761 tgccaggaca tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt
     8821 caagtccata ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt
     8881 cttttggctt acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata
     8941 ttgaaaaatt tattcctcgc caagtaacga gtatcttaag cggtattcgt ccaaaattat
     9001 atggcgaagg aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt
     9061 ggctgatttt gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag
     9121 aagataataa gaccgtgatg gaattgatct tagaaatgat gggccaacca aaagacgcat
     9181 acgaccatgt aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat
     9241 tgcgagaaga gctaggttgg cagcctgagt ttacgaactt ccgtgatggg ttggctgaca
     9301 cgatcaaatg gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag
     9361 cagcatatgc caaaaatgga caataataca gaggaaattt cactgtatta atggaaaaac
     9421 acatgtgagg caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataac
     9481 catatgtttt tattaactgg aggaaatgga caattaggta cagaattgcg ccatttatta
     9541 gatgaaaaaa atctgccata tgtttcaaca gatgcaaaag acttggatat cacagatgct
     9601 gagaaaacaa tggcttatat tacagaatta aaacccgatg ttattttcca ttgcgcagct
     9661 tacacagcag tagataaagc agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc
     9721 gatggcacaa gaaatgtagc acttgcagca aaagctgtga atgcacaatt cgtctatatc
     9781 agtacagact atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa
     9841 acgaatccac taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag
     9901 attttagaag actattatat catacgaact tcttgggttt tcgggatcta tggacacaat
     9961 ttcgtcttca cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac
    10021 cagtttgggc gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa
    10081 gaaaaagcac catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgag
    10141 ttcgcaaaag aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa
    10201 tttccgcaaa aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg
    10261 ttaggttttg tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa
    10321 aaaatgaata agtaatttcc tttttatcaa atagaatttg gagcatgata tggaaaaaca
    10381 aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt cgaatttggt
    10441 gcttgacctg tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt
    10501 tacttttaat ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga
    10561 cgatgggaaa aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg
    10621 gctggtgcag acaccttttt acctctcaca agctgaagag gacttttcgt tggtgaacga
    10681 ggattattca ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat
    10741 tacggttatc ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact
    10801 ttccttttat tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat
    10861 gatattaacg aagaggaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc
    10921 tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg
    10981 actgatgatt gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt
    11041 gatgatgctt ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca
    11101 tcaagtgatg ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag
    11161 caagaaagtt tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat
    11221 tggcggagtc atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa
    11281 taccttatat ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt
    11341 ttctttcctt acaaattgga aaattccttt tgaagatatc aactattcga ataactctga
    11401 agtggccctg ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt
    11461 tggcaaaaaa gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt
    11521 ttctatcttt tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc
    11581 ttatgttcct ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat
    11641 ctggtttatc gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt
    11701 gttggcagta aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta
    11761 tttttcggat tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc
    11821 attatttggc tggagacgct tgttttacct cgcttttgtc gccttagtta tcgtatctgg
    11881 ctttttcgtc aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa
    11941 agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt
    12001 atataacttc accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga
    12061 tatagctgca tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta
    12121 tgctcataca agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga
    12181 tatgttttcg gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat
    12241 cagtaaacga ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata
    12301 tggaccggat aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt
    12361 accacaaaca caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata
    12421 atcctgaaat attagtagaa aggagagtga agataattca agcaatcgta caaaaatttg
    12481 tcaacaaaat gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga
    12541 cagcaggggt aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg
    12601 cttttagtgt ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc
    12661 tttatctagg tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa
    12721 atgagtcttt atttggagac ttactgtttc ctttggttat tttattttgt ttagtaatta
    12781 aatggatcga gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc
    12841 tcggaatcac gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct
    12901 gggctccagg caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt
    12961 tgatgactct tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgttc
    13021 gaatcatcgg tgggatagca gtcgttgcag cctttttagg tatttgggtc tgccaatcta
    13081 gaggtgcatt agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga
    13141 tgcgtgtcat tcgtgcgcca tttattgcct acacggttac gattctttta gctttgccga
    13201 tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt taccgatcga gaagatattt
    13261 ggcataaatt ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa
    13321 catttatctt ccagcgagga aaccaatttt taggaaacca caatagttat aattctattt
    13381 tgaatatcta tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg
    13441 gtcgcttaac gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt
    13501 ttgcggtgat gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga
    13561 ttatttatat cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg
    13621 aattctatgt tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg
    13681 aacaaccaga tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata
    13741 cgagagaggc tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct
    13801 tctgtaaaat agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag
    13861 aacgcagctc ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc
    13921 gcttggagga acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa
    13981 cagaaaatta ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt
    14041 tttaggaatc ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt
    14101 atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt
    14161 agttaccggg atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt
    14221 ttttcgggaa tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac
    14281 gacgaaaatc ctatccaatc tgtacaccca tttattcttc attctgattg gcatagctgt
    14341 cgcgcttata aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt
    14401 ttgcatgtgt gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt
    14461 acctgatatc atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat
    14521 attatggagc cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct
    14581 gtactatgtt gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca
    14641 tggaacatat actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc
    14701 agtgttccgt agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca
    14761 gaatatgcaa ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg
    14821 gatcgatttc gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg
    14881 ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac
    14941 ggatcgggaa aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc
    15001 acgatggaaa tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct
    15061 gagtattctg gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa
    15121 gaagtcgatg aaatggtcga tgatatcatt gaatttgctg acatcggaga ccatatttat
    15181 caacctgtaa aaacttattc aagcgggatg ttcgttcgtc tagcttttgc tgtagcaatc
    15241 aatgtgaatc ccgatatttt gattgtggat gaagcattgg cagtaggcga tcttgaattt
    15301 caactaaaat gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc
    15361 gtttctcatg atatcagttc gattcgtcgt ttttgtgatc gttcctattg gttgaaaaac
    15421 ggagaagtcg tcgaatatgg cgatacgatg gaagtaacga cgaattatga aaacttttta
    15481 aagaaaaaat cagttaaaac agtggatcgg aataaaaacc aagtagaacg gttcgccgct
    15541 ccagatatcg tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa tccggtggat
    15601 atgctggaac aaggagaaaa actatacgtt aaagtaactt atgaagtaaa agatgataca
    15661 atcaaaaatc cggttttagg aattgcgcta cgaacagtcg acaattttta tgtatgcgga
    15721 cttaatactc tactagatgg tatcaagatt ccttggaaaa aaggagagaa tatcttttat
    15781 ctggagtatg aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt
    15841 gaagaaaatg caacggttcc tttggtatat aagacaaagt acatgaactt gtttgtcagc
    15901 ggttcttata tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat
    15961 agcaatgaaa tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc
    16021 cgcacagatc aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gccgattaac
    16081 gaaacattta atcggagcaa aacaatgtga agtgagcatt gttgagttag ataaggaatt
    16141 gttcgatttt gtcagcgaat ttgctcaaga tggattttat ggtgatatcg aaagttttga
    16201 gtgggcaaac tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga
    16261 acatttggta gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg
    16321 agagatcctg attacattcc ctaacttggc gcataattct gtcatgatcg atttgttcaa
    16381 taaccaattg ccgtgggctt cttatggtct cctagatgaa acccataatt ctttttatac
    16441 tcatgatggc ttccaaaaag tttttgaaaa agcgggatta ttcattaata ttgaagatta
    16501 tttgtactta gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc
    16561 tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt
    16621 gatgaaacat ccagtagcac aaagttccat tgcagagcca caaaattcta attatgtaaa
    16681 agtgttagaa gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa
    16741 taatttcaca ggggaaaatg agacactaag tttccccgta tcagaaacaa cagaaagaat
    16801 ggtgtttcgc ttcgcgcaac aacctagctt tatcgaattc agcgcagaag ctgccggaga
    16861 aaaactaaca tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt gttatttgtt
    16921 tgatgggaaa gaattaccag agttcgttct aacggatatt tctgggaaag aagtgactat
    16981 ccattgtcac tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgttggaaac
    17041 gattcgtccc atggctgaag tcacaaagca gctttctgag aaaaatgacg aattagaaag
    17101 agaaaatcat catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac
    17161 aaatcgctat tgcacattgt tggaaagtga tgaatggaca ataaaacata gactaggacg
    17221 acgtaagaaa gaaacttcga aaaaaatcca agaaaaagaa ctttctatat gtattgacga
    17281 gaaaatctgg gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc
    17341 ggatcgccag cccttgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat
    17401 tcctgtttca cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg
    17461 atttgagtta cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca
    17521 aaatggccaa tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt
    17581 aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg
    17641 gccactggcg atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct
    17701 acgataaagg tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat
    17761 atcaatcatt tatataatct tgatgttaag acccaaagcg gtttcaaggt acgtttatca
    17821 gggaaaatga gaggaaaagc catcttagac ttccaaacag cgagacatca aaatggcata
    17881 gctgtgaaat taaacggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga
    17941 aaaaaaagat tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagcg
    18001 atccgtcggg tcaaattgga attgaagcca ggatcgatcg actatgcaga gtggatcagt
    18061 cgtcatgaga aaccagattt gaaagagcaa aggaaagaag cacaaggatt tgcgtaccgt
    18121 ccattgattt ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc
    18181 attgattcgg tactggatca aacgtatgat cattgggaac tatgtatcag tgatgatgct
    18241 tctactgatc cagctatccg aaagtgtctg gaatcctatc aagcaaaaga tgatagaatc
    18301 aaagtcgttt tccgaaaaga aaatggacac atcagtctgg caactaattc tgcattggaa
    18361 atagcagaag gagaatttat agcactctta gataatgatg atgaattacc gccatttgca
    18421 ttatatgaag tggcaaaagt gctaaacgta catccagaac tggatgtgat ctacagtgac
    18481 gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc agactggtcg
    18541 cctgatacat tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagcatt
    18601 gttaaagaac ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta tgatttggtc
    18661 cttcgtgtga cagaacaaat ccctgcagat catatttacc acattgaccg agtcttgtat
    18721 cattggcgga cgatcccagg atcaactgca agtaatggcg aagccaaaag ttatatctat
    18781 gattccggag tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa aggaagcgta
    18841 cgtcctggca gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg
    18901 gtcagtgtga tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg
    18961 atcatcgaaa aaacgtctta ttcaaattat gaaatcatta ttgcggataa cggaagtaca
    19021 gatccaaaaa tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgctttatc
    19081 gtagaattga ttgacattcc attcaattat tctcgaatta acaatttagc agcagaaaaa
    19141 gcaaacggca aatacttttt attcttgaat aacgatacag aagtgattga gccagattgg
    19201 atgacggtga tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg
    19261 ttttatccag atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca
    19321 gggcatgcat tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt
    19381 gacgtcaatt acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat
    19441 gctgttaacg ggtttgatga aacattagaa gtagcattca acgatgttga cttgtgcttg
    19501 aaagtttatg aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc
    19561 gaatctaaat caagaggtta tgaagatact ccagaaaaac aaagacgttt tgctggtgaa
    19621 atcaaaaaaa tgcaagataa atggccagca tatattgcgc atgatccatt ttacaatgat
    19681 aatttaacaa aagacggaat cggcgacttt tcgttgagac cagattaaac tataataaaa
    19741 agctgttatg ttgtgattcg ctcgcacata acggcttttt atagtttttt gtcaaaaaaa
    19801 ggcagaaggt gccgactcaa aaaaataagt cgaggttggg acagaagtgt ttaactccga
    19861 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ggcttattta
    19921 agaagaagtt gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt
    19981 tcttactttt gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag
    20041 ccaaagtttc acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt
    20101 ttaaaataaa tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt
    20161 taaaataaga agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc
    20221 cactattacc aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc
    20281 catatctcat attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta
    20341 tccacatggg tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac
    20401 caatttttgc cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta
    20461 gtggatcaca aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt
    20521 cttcagctgt ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat
    20581 acttattggc aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata
    20641 tgtgtaaaca gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata
    20701 aatctagtga tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt
    20761 tacatttgta gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt
    20821 tctccccgcg tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt
    20881 tgattaatcc ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg
    20941 acatgatatg acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta
    21001 ccccatcctt atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat
    21061 gcaactcttg tttattcata agatattacc tcactttctt ctataagcat aatgtcatta
    21121 aaaatcgatt tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca
    21181 gcgctttggg aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa
    21241 aaagcagcaa atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg
    21301 gagatttgta atttagagtc cttgtcttga ttagaggtgt ataaagcttt ttattagaca
    21361 tttttaaatt ccattacaat ttgcttataa actaggtttt taaaggtgac tatggtaaga
    21421 taatagagat attattttgt gattaggagt gtatatatga ataaaaatgg agaatggagt
    21481 gctgcacgac ggatattcat tatattaatg gatgttattg tatacaatgc agcaatatat
    21541 agtagtttct tactgaagtt tcgtggagag atcccattac gaaatttaga aactttccaa
    21601 cactcagcaa ttattatttc agtgattttt attgcgttga acattttgtt gggtgcatat
    21661 gttttttata atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg
    21721 atgacactag ggatcatggt agtgactttt gcgggccgct ggtttgcttt tccacgtctt
    21781 gttatcttac tttcatttgt tctcggtacg cttttcttaa gtatctatcg tattctcgta
    21841 tataaacttt atttacgcgt aagtcgagat aaaaaggtcg tcattattgg gttagaaaag
    21901 gatgtcgcac cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa
    21961 gcagtcattc ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt
    22021 gtctatcttg cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact
    22081 aaaaatgaaa aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca
    22141 aatattatga atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct
    22201 gaatatgaac tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt
    22261 gcttcaccat ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc
    22321 atctataaac aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc
    22381 tcgatgaccg ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca
    22441 cgagtaacgc ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc
    22501 ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg ttctttcttc
    22561 gtcgatcagt tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt
    22621 atcactgggt atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt
    22681 tttgatttac tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa
    22741 accatcaaga tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg
    22801 atctcattgg cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta
    22861 aaaatgaaca atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt
    22921 ggtcgaacga ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta
    22981 aatgatggat caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa
    23041 cgttttgaat tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata
    23101 agacagtcaa aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg
    23161 atcttgtcag attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt
    23221 tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt
    23281 acagaatgtc actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat
    23341 gaacagttga tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac
    23401 caaattctat ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat
    23461 gaatactgtc atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt
    23521 gggaagggga tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat
    23581 tcattagcaa atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta
    23641 aaagggacaa cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag
    23701 aagaaagacg aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt
    23761 gctaagacgg attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca
    23821 ctatttttat ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg
    23881 gctgctttga agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat
    23941 tttcagggac gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa
    24001 attgatcgta caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa
    24061 gactatcatt cagagattga aagattagga ggccgtattt ttgttgtacc taatcctaag
    24121 aa
//
LOCUS       DAHUWM010000018        23831 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHUWM010000018 DAHUWM010000000
VERSION     DAHUWM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.018"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(55..789)
                     /locus_tag="NK788_000510"
     CDS             complement(55..789)
                     /locus_tag="NK788_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCH6768862.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="NK788_000511"
     CDS             1116..1526
                     /locus_tag="NK788_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCH6768863.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="NK788_000512"
     CDS             1519..2220
                     /locus_tag="NK788_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCH6768864.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="NK788_000513"
     CDS             2220..3806
                     /locus_tag="NK788_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768865.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="NK788_000514"
     CDS             3825..4046
                     /locus_tag="NK788_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCH6768866.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="NK788_000515"
     CDS             4043..4804
                     /locus_tag="NK788_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCH6768867.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="NK788_000516"
     CDS             5184..5693
                     /locus_tag="NK788_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCH6768868.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="NK788_000517"
     CDS             complement(5768..6307)
                     /locus_tag="NK788_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCH6768869.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="NK788_000518"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="NK788_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCH6768870.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="NK788_000519"
     CDS             7320..8171
                     /locus_tag="NK788_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCH6768871.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="NK788_000520"
     CDS             8199..8834
                     /locus_tag="NK788_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCH6768872.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="NK788_000521"
     CDS             8854..9627
                     /locus_tag="NK788_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6768873.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="NK788_000522"
     CDS             10035..10832
                     /locus_tag="NK788_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCH6768874.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="NK788_000523"
     CDS             10810..11223
                     /locus_tag="NK788_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCH6768875.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="NK788_000524"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="NK788_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCH6768876.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="NK788_000525"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="NK788_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCH6768877.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="NK788_000526"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="NK788_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCH6768878.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="NK788_000527"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="NK788_000527"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCH6768879.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="NK788_000528"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="NK788_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCH6768880.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="NK788_000529"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="NK788_000529"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCH6768881.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="NK788_000530"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="NK788_000530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCH6768882.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="NK788_000531"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="NK788_000531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCH6768883.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="NK788_000532"
     CDS             21907..22485
                     /locus_tag="NK788_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6768884.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="NK788_000533"
     CDS             22537..23019
                     /locus_tag="NK788_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCH6768885.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="NK788_000534"
     CDS             23136..23510
                     /locus_tag="NK788_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768886.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAHUWM010000019        23445 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHUWM010000019 DAHUWM010000000
VERSION     DAHUWM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.019"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            39..851
                     /locus_tag="NK788_000535"
     CDS             39..851
                     /locus_tag="NK788_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCH6768887.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            848..1648
                     /locus_tag="NK788_000536"
     CDS             848..1648
                     /locus_tag="NK788_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCH6768888.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1809..3077
                     /gene="rseP"
                     /locus_tag="NK788_000537"
     CDS             1809..3077
                     /gene="rseP"
                     /locus_tag="NK788_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCH6768889.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3145..4854
                     /locus_tag="NK788_000538"
     CDS             3145..4854
                     /locus_tag="NK788_000538"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCH6768890.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5066..9424
                     /locus_tag="NK788_000539"
     CDS             5066..9424
                     /locus_tag="NK788_000539"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCH6768891.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9569..10042
                     /gene="rimP"
                     /locus_tag="NK788_000540"
     CDS             9569..10042
                     /gene="rimP"
                     /locus_tag="NK788_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCH6768892.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10065..11240
                     /gene="nusA"
                     /locus_tag="NK788_000541"
     CDS             10065..11240
                     /gene="nusA"
                     /locus_tag="NK788_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCH6768893.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11262..11555
                     /locus_tag="NK788_000542"
     CDS             11262..11555
                     /locus_tag="NK788_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCH6768894.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11552..11863
                     /locus_tag="NK788_000543"
     CDS             11552..11863
                     /locus_tag="NK788_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCH6768895.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11876..14182
                     /gene="infB"
                     /locus_tag="NK788_000544"
     CDS             11876..14182
                     /gene="infB"
                     /locus_tag="NK788_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCH6768896.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14209..14556
                     /gene="rbfA"
                     /locus_tag="NK788_000545"
     CDS             14209..14556
                     /gene="rbfA"
                     /locus_tag="NK788_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCH6768897.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14713..14886)
                     /locus_tag="NK788_000546"
     CDS             complement(14713..14886)
                     /locus_tag="NK788_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6768898.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15334..17696
                     /locus_tag="NK788_000547"
                     /pseudo
     CDS             15334..17696
                     /locus_tag="NK788_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18064..18987
                     /gene="truB"
                     /locus_tag="NK788_000548"
     CDS             18064..18987
                     /gene="truB"
                     /locus_tag="NK788_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCH6768899.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18991..19932
                     /gene="ribF"
                     /locus_tag="NK788_000549"
     CDS             18991..19932
                     /gene="ribF"
                     /locus_tag="NK788_000549"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCH6768900.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20284..21069
                     /locus_tag="NK788_000550"
     CDS             20284..21069
                     /locus_tag="NK788_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6768901.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21082..21933
                     /locus_tag="NK788_000551"
     CDS             21082..21933
                     /locus_tag="NK788_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCH6768902.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22057..22509)
                     /locus_tag="NK788_000552"
     CDS             complement(22057..22509)
                     /locus_tag="NK788_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6768903.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22974..23342)
                     /locus_tag="NK788_000553"
     CDS             complement(22974..23342)
                     /locus_tag="NK788_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCH6768904.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         7948 a         4074 c         5084 g         6339 t
ORIGIN      
        1 aagatagtgg gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa
       61 aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc
      121 agcacattgc catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac
      181 gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa
      241 ggcttggagt gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa
      301 aagatgaagt aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag
      361 aattgattaa agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac
      421 atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat
      481 tgaattttgc tttgaattat ggaagcagag cagagatcgt gactgctgtc caagaaattg
      541 ccgaagaagt agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta
      601 agcatttaat gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta
      661 caagtggaga agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt
      721 attttacaaa agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct
      781 cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg
      841 atcagtcatg aaacaacgtg tggtcacagc agccgtggcg ctagtcttgt ttattccgat
      901 tatatggatc ggaggcatag cggttgagct tacagcagca atcttagcag taataggagt
      961 ttacgagctg tttcggatga aaggattgac actcttaagt tttgaaggag ttttatccgc
     1021 cgttggtgca gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa
     1081 tgtaagtaca tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat
     1141 ttctaaaaat acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt
     1201 gggaataggt ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt
     1261 tggtctattt attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg
     1321 tcagcataag ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg
     1381 tattggtagt gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact
     1441 atttaatcat ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca
     1501 atttggtgat ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa
     1561 tatcttacca ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc
     1621 tatcatgcat ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat
     1681 aaaagacgca ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat
     1741 ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga
     1801 aaaaagctat gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc
     1861 atgagtttgg tcatttcttc tttgcgaaac gatcaggaat cctcgttcgc gaatttgcta
     1921 tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc
     1981 gcctattacc tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa
     2041 tggcaccagg aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa
     2101 atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt
     2161 tagaagatga gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct
     2221 accctgttga tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc
     2281 caaaagacgt acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg
     2341 gacccatgaa taactttatt ttggcaatcc tgttattcat cattctggca tttatgcaag
     2401 gtggtgttca agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg
     2461 cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt
     2521 catggaatga tctgacaaca gtcattacga aaaatccgga taaaacatta gactttaaga
     2581 tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg
     2641 gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga
     2701 tcattggagg gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat
     2761 cactatttac aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt
     2821 cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa
     2881 tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct
     2941 tgaatatttt tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga
     3001 cattagctgg ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc
     3061 aacgattctt tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt
     3121 tgaggaaata aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc
     3181 gaagttccaa atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc
     3241 cgccaagtat cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa
     3301 ctgaaacgca ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct
     3361 gccatcttgc cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac
     3421 ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa
     3481 acttttactg atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt
     3541 tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga
     3601 agagagttta tcatgaaaga tgcgtattct ttccatgcaa cggaagaaag tttggacgag
     3661 acatataaag attacgaaaa agcctacacc gaagtattca agcgttgtgg cttagaattt
     3721 cgctcgatta tcggagacgg tggcgcaatg ggcgggaaag actcaaaaga attcatggcg
     3781 atctcagaaa tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac
     3841 ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg
     3901 gagaaagtag ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt
     3961 gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt
     4021 ttggtacgcg gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac
     4081 tttttagaag aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct
     4141 gtcggaccag tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat
     4201 ttagcgaatg ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca
     4261 gaacgcgatt tcactgtact ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct
     4321 tcaccagaca acaatggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatcttc
     4381 aaaatcggca ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga
     4441 gaaaaatttg tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc
     4501 gtagaacaac attcagatga acaaggaatc aactggccaa aaggaattgc gccatttgat
     4561 ttgcatatcg tccaaatgaa tctaaaagac gaataccaaa caaacctgac gaatgaacta
     4621 gaagaagcga tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca
     4681 ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa
     4741 aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta
     4801 cgtaaagaag aattggtgaa tactttacca atcctcttga accaaggcga ataataagcg
     4861 ttaaaacaca gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct
     4921 tttaaaattg gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa
     4981 aaacgagtat actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt
     5041 ttctcatcag aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt
     5101 ggaacaaatc caactagagg aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat
     5161 ggatagtgta gtggtccatc gtaaatcgcg tttatgggaa tttacgctgc atttttccaa
     5221 aattttgcct attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga
     5281 tattgcacaa gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt
     5341 gcaagattac tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca
     5401 agtattgaaa acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag
     5461 tacaggagct atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt
     5521 cagttacgga ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg
     5581 agtattgaaa ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc
     5641 agctgaaagt ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc
     5701 tcttgaagga cccatccaca tccagctagg acgaaatatt ccagccgatg aaccaacgac
     5761 accaatgatc agtatcgttg aagaagaacg aagagtcaca cttgaggggt acgtattcga
     5821 caaagaagtc agagaactac gttcaaaacg caagatcttg actttgaaaa tcacagatta
     5881 tacttcatca tttatcgtca aaaaattctc gaacggcgaa aaagatgaac aagtatttga
     5941 tgcgatcagt gtcggtagct ggttaaaggt acgaggaagt attcaagaag atacgtttgt
     6001 ccgtgatttg gtcatgaatg cgcaagatat catagaagtc aaacatacac ctcgcaaaga
     6061 ttatgctcct gaaggggaaa aacgagtaga gcttcatgtt catagcaata tgagtacgat
     6121 ggatgcaaca aacagcatct ctgatcttgt ggcacaagca gggaaatggg gacaccgagc
     6181 tatcgccatt acagaccacg gtggtgcgca agcctttccg gaagcacata gtgctggaaa
     6241 aaaagcaggt gtgaaaatct tatacggcgt ggaagcgaat gtcgtagatg atggtgtccc
     6301 aattgcttat aatgatgcac atgaagcatt gagtgaagcc acatatgtcg tatttgacgt
     6361 ggaaacgaca ggattgtctg cagtatatga cacgatcatc gagctagccg cagtcaaaat
     6421 gtataaagga aatgtgatcg aaagctttga tgaatttatc gatccaggac atcctttgtc
     6481 acgaacaacg gtagatctga ctggtatcac ggatggaatg gttcgaggat caaagtcgga
     6541 agaagaagta ttgcggatgt tccttgaatt ttcaaaagat acaattttgg tagcccacaa
     6601 cgcggctttt gatatggggt tcttgaatac tagctacgca aggtatggta ttcctgaagc
     6661 ggccaatcca gtcattgata cgttggaatt agctagatat ctgtatcctc aattcaaacg
     6721 tttcggatta ggtgtgttat ctaaaaaatt tggtgtcagc ttggaacagc atcaccgagc
     6781 aatctatgat gctgaagcaa ctggtcactt agcatggatc tttgtgaaag aggcaatgga
     6841 caatcataac atgctttatc atgatcagct aaatgagcat atcggagaag gagattctta
     6901 caaaagggct cgacctttcc atgtaacgat tttagcaaaa aatcaggcag gactgaaaga
     6961 tttgttcaaa ctgatttcaa tgtcgaatgt cgaatacttc gaacgcgtcc cacgaatacc
     7021 acgctcccaa ttgaaaaaga tgcgtgaaaa tctattgatc ggctcagctt gtgataaggg
     7081 agaaatattc gaagccatga tgcaaaaagg ggtagaagag gcaagaaacc gagcaaaatt
     7141 ctacgattat attgaagtta tgccaaaagc ggtctatgcg cctttgatcg agcaagaatt
     7201 ggtaaaaaat gaacatgatt tggaagaaat tatccaaaat ttggtagaaa tcggaaaaag
     7261 tttggataaa atcgttgttg caacaggtaa tgtacattat ctgaacgaag aagatgccat
     7321 ttaccgaaaa attttgatca attctatggg cggagcgaat ccgttgaatc gtcatagttt
     7381 gccggatgtc catttccgaa caaccgatga gatgctaact gcatttcact ttttagggga
     7441 agaaacagcg aaagagatcg tagtagaaaa tacgaataaa atcgcagata tctgcgaaga
     7501 agtgattcca gtcaaagatg aactttatac accgaagatt ccaggttcag aagatgaaat
     7561 atcagaattg agttacacaa aagcaaaaca aatgtacgga gatccgttac ctgagatcat
     7621 tcaaaaacga ttaaaaaaag agttgaattc tattaatggt aatggcttct cggttattta
     7681 tctgatcgcc caaaaactag tgcataaaag taatgaagat ggctatctag taggttcacg
     7741 tggctctgtt ggctctagct ttgttgcgac gatgacagga atcactgaag ttaatccgtt
     7801 agcccctcat tattattgtc cagagtgcca atattccgag ttttttgaag atggtacgta
     7861 tggttctgga tttgacatgc cagaaaaaca atgtccaaaa tgtggtgcac gcttaaacaa
     7921 agacggtcat gatattccat ttgagacatt tttaggattc catggagaca aagtgcccga
     7981 tattgatttg aacttctcag gtgattatca agcagaagcc cataactata cgaaagtatt
     8041 gtttggcgaa gactatgttt accgtgccgg aacgattggt acagtcgccg acaaaacagc
     8101 atatggctat gtaaaaggat acgaaaggga caataatctg cagtttcgaa gtgcagaagt
     8161 cgacagatta gcaaaaggcg ctactggagt caaacggaca accggccagc atccaggggg
     8221 gatcatcgtt attcccgatt atatggatgt ctatgatttt acgccgatcc aatatccagc
     8281 agacgatcaa aattcggaat ggaaaacgac tcattttgat tttcactcga ttcacgataa
     8341 tgtattgaaa ttagatattc tagggcatga tgacccaact gttattcgga tgcttcaaga
     8401 tttatcaggg atcgatccac aaacgatccc tacggatgac ccggaagtga tgcggatatt
     8461 tgccggtcct gaagtacttg gagtaagtca ggaacaaatt tattccaaaa cagggacatt
     8521 aggtattcca gaattcggca ctcgttttgt tcgagggatg ctagaagaaa cgcatcctac
     8581 aacttttgca gaactactgc aaatttccgg actatctcat ggtacagacg tatggttagg
     8641 aaatgcggaa gaattgattc gacgaggaga tgcgacactg gcggaagtaa tcggctgtcg
     8701 tgatgatatt atggtttatt tgatccatgc tggattagat agcgggatgg catttaaaat
     8761 catggaaact gttcgtaaag gacaatggaa caagatccct gatgaattgc gagaaaccta
     8821 tctatcggcg atgaaagaaa ataatgtgcc cgactggtat atcgattctt gttcaaaaat
     8881 caaatatatg tttccgaaag cccatgctgc agcttacgta ttgatggctc ttcgtgtcgc
     8941 ttatttcaag gtttatttcc ctattttgta ttactgtgca tacttttctg tacgtgcaga
     9001 cgactttgat ctagtctcca tgtgcaaagg aaaagatgct gtgaaacaag caatgaaaga
     9061 aatcacggac aaaggattag atgcttccgt aaaagaaaag aatcaactga cagtgctgga
     9121 gttagccaat gaaatgctgg aacgaggatt caaatttgga atgattgatt tgtataaatc
     9181 ggatgccgtt aactttgtta ttgaaggtga tacgttgatc gcaccattta gagctgtccc
     9241 aagtttggga accaacgtgg caaaacaaat cgttgaagca agaaaagacg gtccattttt
     9301 atccaaagaa gatcttgcca cccgcggtaa agtttcaaaa acattgatcg aatatatgaa
     9361 cgacaatggc gtattgaaag atctaccaga tgaaaatcaa ttatcgttat tcgatatgct
     9421 ataagcatct gttttcctgt ctgcttgtaa ttttaagcag agtatggtat agttcttata
     9481 ggtaatgata ctaactgtga gtgagcggat ttttcgctca ctctttttaa tggaattacg
     9541 cacttaattt tttaaggagg cgaaactttt gagtagcgtc gttgaaacag tcacagaaat
     9601 ggtgacaccg attttagatg aacaaaattt tgaacttgtc gaagtcgaat ttgtaaaaga
     9661 aggaaaaagc tggttcttac gagtatttat tgacaaagaa ggcggtatcg atatcgaaga
     9721 atgcgccttt gtcagtgaga aactgagtga aaaacttgat gcagcagatc cagatccgat
     9781 tcctcaagct tatttcctgg aggtatcctc cccgggagca gagcggccat tgaaaaaaga
     9841 aagcgactat gaaaaggcac ttggtgatta tgtccatgtc tccttatacc aaccagtaga
     9901 tggcgaaaaa caatatgaag gatttcttca atcatttgat gctgaacagc ttacattgaa
     9961 aatacgcata aaaacacgag aaaaagaatt gacttttgac cgaaaaaata ttgcaaaagc
    10021 tcgcttagct atccaatttt aatcacggag aggaatcaga aaaaatgagt aaagaaatgt
    10081 taaatgcgtt agacgcttta gaagctgaaa aagggatctc aaaagaaatc gtgatcgatg
    10141 ctttagaagc agcattggtt tctgcataca aacgtcacta tggccaagca caaaacgtcg
    10201 aagtggaatt tgatcagaaa aaaggaaaga tccatgtcta tgcggtaaaa gaagtaacgg
    10261 aagaagtgat ggattcacag ttggaagtct cattgaaaga tgcactttta atcaatccag
    10321 catatgagat cggtgatacg atccgttttg aagtaacacc aaaagatttt gggcggatcg
    10381 ctgcgcaaac ggctaaacaa gttattttac agcgtgtacg tgaagcagaa agaacgatta
    10441 tttataacga attcagtgct tatgaaaaag acatcatgca aggaatcgtc gaacgccagg
    10501 ataaacgtta tatttatgtc aatcttggta aaattgaggc agttctttct aaacaagatc
    10561 aaatgccaaa tgaattttat caaccacatg atcgaatcaa agtttatgtt tcccgagtag
    10621 aaaatacgtc taaaggtccg caagtatttg ttagccgcag ccatccagac ctattgcgcc
    10681 gcttatttga gcaagaagta ccagaagtct atgatggaat cgttgaaatt gtcagcatcg
    10741 cccgtgaagc tggcgatcgt tcaaaagtgg cagttcgctc aaatgatcca aatatcgatg
    10801 cagtcggtac atgtgtcggg ccaaagggac aacgagttca agcaatcgtc aatgaattaa
    10861 aaggcgaaaa catggatatc gtcgaatgga atgaagatcc agctgtattc attgcaaatg
    10921 cattgaatcc tgcacaagtc atggatgtga tcttcgatga gacaaatcca aaggcctgca
    10981 cagttgtcgt tccagactac caattatcgc tagcaatcgg taaacgcgga caaaacgcac
    11041 gtttagctgc taaattgact aaccataaaa tcgatatcaa atcagaatct gacatgacgg
    11101 aattttatga aaatcaagaa caacaaaaag aaacagaaga actgcatgat gaagcaatcg
    11161 ttcagtcaga tttaacagac gatgaatatg aaacaattgc attcaacaat gaaacggtcg
    11221 aagaaaaacc agaagcttaa ctttagtaag gaggcaaaga gatgaaacaa aggaaaattc
    11281 ctttacgcaa atccgtggtt tcaggtgaaa tgaaacctaa aaaagagatg gtccgaatca
    11341 cccgttcaaa agaaggggaa gtatcgatcg atccaactgg aaaaatgcct ggacgaggag
    11401 cgtatgtctc acttgagccg gaagaagtac aacaagcttg ggataaaaaa attctcgatc
    11461 gagtactcga aacaacatta acagatgagt tttatcaaga acttcttgac tatgtcactc
    11521 accaaaaagc acgtaaagag ttatttggaa aatgaataca gttaatcggc aaaaagccat
    11581 gaatctcatc ggtctagcga tgcgcgcggg aaaactaatc acgggtgaag aactgaccat
    11641 tgctgaaatc cgcaaacaaa aagcaaaatt ggtgtttgtg gcaaccgatg caagcgagaa
    11701 tacaagaaaa aagataaaag ataagagttc atattatgaa gttccttgct ttgagctatt
    11761 ttctgaagca gaaatcacgc agatgatcgg aaaacctcgc aaggtattcg gaataatgga
    11821 tgccggattt gcgaagaaga ccaaggagct aattgaaggt taggaaggtg attgcatggg
    11881 caacaaaaga atctatgaat tagcaaaaga actcaaaaaa tctagtaaag acgtcgtgga
    11941 aaaagcacaa caacttggca tcgatgtgaa aaaccatatg ggtgcgatta ctagcaatga
    12001 tgagaagaaa ttgcaacaag cgttcaaaaa cccaagcacg aaacaacaag ctgcacaaca
    12061 agcaagcaga aaaccagcca atcaacagcc gaacgatcaa cgtaaaaaag aaacaacgaa
    12121 caatcaacaa aaaaataaaa ataaccgcag ttatcaagat cgcggtcaag ggagcggtca
    12181 agtgaatcaa ggaaaaaaac caacaacaaa ccaaaataat acgggaaata ccagcagcca
    12241 aaatcgtcaa ggaacgagcc aaggcggcaa tcaaaaccgt actaacaata acaacaaacc
    12301 aaacaataac aatcgcggca aatttaataa taataaccgt aatcgttata ataaaaaagg
    12361 taaaaaaggc aaacagcaac aatcaactaa accagctgta ccaccacgca aattccgtga
    12421 attgccagat gtattagagt atactgaagg tatgaacgta gcagatattg cgaaaaaaat
    12481 tcatcgtgag ccagcagaaa tcattaaaaa attatttatg cttggtgtca tggtcaatca
    12541 aaaccaagca ctggataaag atacgattga actgttagcg acggactatg gcatggaacc
    12601 acaagaaaaa gtccaagttg acattgctga tatcgataaa ttcttcgagg cagaagaagt
    12661 cgatccagat aaattagtct cacgtccgcc agtcgtaacg attatgggac acgtagacca
    12721 tgggaaaaca actttattgg atacattacg ccattcacgt gtaacaagcg gcgaagccgg
    12781 cggaatcaca cagcatatcg gtgcctacca gatcgatatt gatggtaaac caatcacttt
    12841 cttggataca ccaggacatg cggcctttac aagtatgcgt gcacgtggag caagtatcac
    12901 agatatcacg atcttagttg ttgctgcaga tgatggcgtc atgccacaaa cagttgaagc
    12961 aatcaaccat gccaaagctg caggcgttcc aatcatcgtc gctgtaaata aaattgataa
    13021 accaggtgca aaccctcaac atgttatgca agaattaagt gagtatgagt taattcctga
    13081 agcatggggt ggagaaacga tctttgttga gatctcagcg aaattcggcc aaaacatcga
    13141 agaattgctg gaaatgatcc tgttagtcgc agaagtagaa gatttaaaag cagatccaac
    13201 acaacgcgca atcggaacag tgatcgaagc acgtcttgat aaaggtaaag gtcctgtgtc
    13261 aaccttactt gtccaagaag gaacacttca tgttggtgac ccaatcgttg tcggtaatac
    13321 ttacggacgt gtccgtgtaa tgacaaacga tcttggccgt cgcgataaag aagcaggacc
    13381 tgcaacgcca gtagaaatca ctggcttgaa tgatgtacct caagcaggtg accgtttcgt
    13441 agtcttcgaa gacgaaaaga cagcacgtgc agcgggtgaa gaacgcgcga aacgtgcatt
    13501 attagaaaac cgtgctgcca caagccgtgt gactttggat aacttatttg aaagcctgaa
    13561 agaaggcgaa ttgaaagaag ttaacgtaat catcaaagca gatgtacaag gttcagcaga
    13621 agcactggct gcatcattga agaagattga tgtggaaggt gtccgtgtaa aaatcgtcca
    13681 ttcagctgtc ggagcaatca atgaaagtga tgtaacctta gcagcagcca gcaacgctat
    13741 tatcatcggt ttcaacgttc gtcctacacc gcaggcaaaa gtgcaagctg atactgaaga
    13801 agtcgatatt cgtcttcacc gtatcatcta taaagccatc gaagaaatcg aaactgcgat
    13861 gaaagggatg ctagaccctg aatttgaaga aaaaatcacc ggtcaaatga tcgttcgtga
    13921 aaccttcaaa gtttctaaag ttggtacgat tgcaggtgcc tatgtaacag aaggatatat
    13981 tcgtagagat agcggtgtcc gtgtaatccg tgacggaatc gtaatctatg aaggccaact
    14041 tgcaagtctg aaacgattca aagatgacgt gaaagaagtc aaaatgggtt atgaatgtgg
    14101 tgcaatgatt gaaaaattca atgacatcaa agtagatgac gtaattgaag gatttatcat
    14161 ggaagaagtc aaaaatgact aaaaactgat aaaaggaggc caatcattat ggctaactac
    14221 cgtgaccgcc gagtcggtca agaaatctta aaagaagtca atgatatttt gcgcaaaaag
    14281 gtccgtgacc ctcgtgtgga gaacgtaacg atcacagatg tgaaagttac gggggactta
    14341 cagcaagcaa cgatctatta cagcatttta tctgacttag catcagataa gaaaacagca
    14401 caagatggat tgaataaagc atcaggattg attcgtcgag agttagggca caatctaagt
    14461 atttataaga cacctgaatt gttctttgaa cttgatgaat cagttgtcta tggcaaccat
    14521 atcgatgaat tgcttcgcga cttgaacaaa gactaatcaa aaactcccgc aaatcctgtg
    14581 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
    14641 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
    14701 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
    14761 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
    14821 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
    14881 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
    14941 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
    15001 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
    15061 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
    15121 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
    15181 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
    15241 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
    15301 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
    15361 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
    15421 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
    15481 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
    15541 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
    15601 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
    15661 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
    15721 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
    15781 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
    15841 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
    15901 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
    15961 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
    16021 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
    16081 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
    16141 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
    16201 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
    16261 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
    16321 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
    16381 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
    16441 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
    16501 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
    16561 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
    16621 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
    16681 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
    16741 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
    16801 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
    16861 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
    16921 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
    16981 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
    17041 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
    17101 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
    17161 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
    17221 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
    17281 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
    17341 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
    17401 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
    17461 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
    17521 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
    17581 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
    17641 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
    17701 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
    17761 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
    17821 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
    17881 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
    17941 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
    18001 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
    18061 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
    18121 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
    18181 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
    18241 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
    18301 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
    18361 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
    18421 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
    18481 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
    18541 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
    18601 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
    18661 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
    18721 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
    18781 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
    18841 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
    18901 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
    18961 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
    19021 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
    19081 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
    19141 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
    19201 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
    19261 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
    19321 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
    19381 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
    19441 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
    19501 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
    19561 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
    19621 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
    19681 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
    19741 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
    19801 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
    19861 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
    19921 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
    19981 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
    20041 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
    20101 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
    20161 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
    20221 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
    20281 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
    20341 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
    20401 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
    20461 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
    20521 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
    20581 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
    20641 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
    20701 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
    20761 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
    20821 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
    20881 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
    20941 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
    21001 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
    21061 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
    21121 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
    21181 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
    21241 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
    21301 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
    21361 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
    21421 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
    21481 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
    21541 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
    21601 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
    21661 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
    21721 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
    21781 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
    21841 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
    21901 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
    21961 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
    22021 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
    22081 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
    22141 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
    22201 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
    22261 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
    22321 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
    22381 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
    22441 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
    22501 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
    22561 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
    22621 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
    22681 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
    22741 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactatgt agaaaattca
    22801 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
    22861 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
    22921 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
    22981 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
    23041 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
    23101 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
    23161 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
    23221 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
    23281 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
    23341 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
    23401 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tattt
//
LOCUS       DAHUWM010000020        22811 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHUWM010000020 DAHUWM010000000
VERSION     DAHUWM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.020"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            19..243
                     /gene="nrdH"
                     /locus_tag="NK788_000554"
     CDS             19..243
                     /gene="nrdH"
                     /locus_tag="NK788_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCH6768905.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            402..2561
                     /gene="nrdE"
                     /locus_tag="NK788_000555"
     CDS             402..2561
                     /gene="nrdE"
                     /locus_tag="NK788_000555"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCH6768906.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2611..3576
                     /gene="nrdF"
                     /locus_tag="NK788_000556"
     CDS             2611..3576
                     /gene="nrdF"
                     /locus_tag="NK788_000556"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCH6768907.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3665..4804)
                     /locus_tag="NK788_000557"
     CDS             complement(3665..4804)
                     /locus_tag="NK788_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6768908.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5113..5826)
                     /locus_tag="NK788_000558"
     CDS             complement(5113..5826)
                     /locus_tag="NK788_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCH6768909.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6080..6781)
                     /gene="nagB"
                     /locus_tag="NK788_000559"
     CDS             complement(6080..6781)
                     /gene="nagB"
                     /locus_tag="NK788_000559"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCH6768910.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7015..8547
                     /locus_tag="NK788_000560"
     CDS             7015..8547
                     /locus_tag="NK788_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6768911.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8738..9940)
                     /locus_tag="NK788_000561"
     CDS             complement(8738..9940)
                     /locus_tag="NK788_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6768912.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10022..10711)
                     /locus_tag="NK788_000562"
     CDS             complement(10022..10711)
                     /locus_tag="NK788_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCH6768913.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10817..11185)
                     /gene="rplL"
                     /locus_tag="NK788_000563"
     CDS             complement(10817..11185)
                     /gene="rplL"
                     /locus_tag="NK788_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCH6768914.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11238..11741)
                     /locus_tag="NK788_000564"
     CDS             complement(11238..11741)
                     /locus_tag="NK788_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCH6768915.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11945..12072)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12151..12840)
                     /gene="rplA"
                     /locus_tag="NK788_000565"
     CDS             complement(12151..12840)
                     /gene="rplA"
                     /locus_tag="NK788_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCH6768916.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12944..13366)
                     /gene="rplK"
                     /locus_tag="NK788_000566"
     CDS             complement(12944..13366)
                     /gene="rplK"
                     /locus_tag="NK788_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCH6768917.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13709..14377
                     /gene="sdaAB"
                     /locus_tag="NK788_000567"
     CDS             13709..14377
                     /gene="sdaAB"
                     /locus_tag="NK788_000567"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCH6768918.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14390..15262
                     /gene="sdaAA"
                     /locus_tag="NK788_000568"
     CDS             14390..15262
                     /gene="sdaAA"
                     /locus_tag="NK788_000568"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCH6768919.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15353..16372)
                     /locus_tag="NK788_000569"
     CDS             complement(15353..16372)
                     /locus_tag="NK788_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCH6768920.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16545..17450)
                     /locus_tag="NK788_000570"
     CDS             complement(16545..17450)
                     /locus_tag="NK788_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6768921.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(17509..18054)
                     /gene="nusG"
                     /locus_tag="NK788_000571"
     CDS             complement(17509..18054)
                     /gene="nusG"
                     /locus_tag="NK788_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCH6768922.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18155..18325)
                     /gene="secE"
                     /locus_tag="NK788_000572"
     CDS             complement(18155..18325)
                     /gene="secE"
                     /locus_tag="NK788_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCH6768923.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18349..18501)
                     /gene="rpmG"
                     /locus_tag="NK788_000573"
     CDS             complement(18349..18501)
                     /gene="rpmG"
                     /locus_tag="NK788_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCH6768924.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18663..19307
                     /locus_tag="NK788_000574"
     CDS             18663..19307
                     /locus_tag="NK788_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCH6768925.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19363..19866)
                     /locus_tag="NK788_000575"
     CDS             complement(19363..19866)
                     /locus_tag="NK788_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCH6768926.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19879..19925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20463..21428
                     /locus_tag="NK788_000576"
     CDS             20463..21428
                     /locus_tag="NK788_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCH6768927.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21527..22699)
                     /locus_tag="NK788_000577"
     CDS             complement(21527..22699)
                     /locus_tag="NK788_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6768928.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22686..>22811)
                     /locus_tag="NK788_000578"
     CDS             complement(22686..>22811)
                     /locus_tag="NK788_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6768929.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7128 a         4584 c         4102 g         6997 t
ORIGIN      
        1 ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt
       61 aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat
      121 gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt
      181 acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc
      241 taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca
      301 gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca
      361 atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac
      421 aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc
      481 ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata
      541 cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag
      601 aagcagaatt cattcaattg tattcaccat catttattga aaagctatat caatttttgg
      661 acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg
      721 cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca
      781 atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga
      841 tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg
      901 gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct
      961 caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa
     1021 atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc
     1081 cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag
     1141 gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa
     1201 aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac
     1261 ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata
     1321 gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc
     1381 atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg
     1441 aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga
     1501 accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac
     1561 aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta ggaacggata
     1621 tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc
     1681 aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg
     1741 tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg
     1801 gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact
     1861 ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg
     1921 ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt
     1981 atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt
     2041 ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga
     2101 aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata
     2161 tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga
     2221 agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt
     2281 acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca atggcacaac
     2341 aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca
     2401 tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt
     2461 acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag
     2521 aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct
     2581 tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac
     2641 tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt
     2701 tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa
     2761 ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa
     2821 tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt
     2881 ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt
     2941 acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat
     3001 ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag
     3061 aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt
     3121 tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga
     3181 gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta
     3241 cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac
     3301 gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc
     3361 aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc
     3421 tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca
     3481 agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg
     3541 atgaaagacg acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt
     3601 ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt
     3661 atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt
     3721 ccattcttct caataaatag attttccgga tatttaatat tatcgtctta atcactgcat
     3781 agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata
     3841 ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca
     3901 aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg
     3961 gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg
     4021 ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata
     4081 ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga
     4141 tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg
     4201 ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata
     4261 gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt
     4321 ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat
     4381 ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt
     4441 catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg
     4501 tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa atgattacga
     4561 ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa
     4621 cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa aaaactgccg
     4681 aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga
     4741 tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt
     4801 gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg
     4861 cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga
     4921 cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt
     4981 ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa
     5041 tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca
     5101 acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac
     5161 ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat
     5221 atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac
     5281 gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa
     5341 ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc
     5401 aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat
     5461 ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt
     5521 cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag
     5581 ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa
     5641 tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa
     5701 aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc
     5761 gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc
     5821 attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt
     5881 gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta
     5941 tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg
     6001 ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
     6061 tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa
     6121 cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca
     6181 tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt
     6241 tcataattga accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga
     6301 agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt
     6361 caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt
     6421 ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat
     6481 cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga
     6541 catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta
     6601 agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag
     6661 taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt
     6721 tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca
     6781 tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt tttcttttta
     6841 caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc
     6901 gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag
     6961 tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt
     7021 atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc
     7081 gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa
     7141 agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg
     7201 caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca
     7261 atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct
     7321 tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa
     7381 ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg
     7441 agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt
     7501 ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc
     7561 ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt
     7621 caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc
     7681 accacccata tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa
     7741 caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa
     7801 caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca
     7861 acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct
     7921 tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg
     7981 gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc
     8041 atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta
     8101 ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac
     8161 acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga aacaccacta
     8221 caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct
     8281 aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa
     8341 caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat
     8401 gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa
     8461 tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa
     8521 cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg
     8581 tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt
     8641 cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt
     8701 acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc
     8761 tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc
     8821 taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag
     8881 ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg cttgctgctt
     8941 cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc ccgacgtcta
     9001 ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat aaagcaataa
     9061 agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa taagaatctg
     9121 ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc gctaatatcc
     9181 ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc
     9241 aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct cttaatagac
     9301 gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa acagctgtac
     9361 tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga attccgactg
     9421 cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca ataccagata
     9481 ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc atgaccttcc
     9541 ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg
     9601 aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag
     9661 cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt ggacgatcca
     9721 ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat tgggtcaaca
     9781 tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact tttgttccaa
     9841 tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat aatagtaaaa
     9901 taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc catttcctat
     9961 cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt cgccacactt
    10021 attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg aagacaacta
    10081 accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta tatccgtgaa
    10141 acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca atcacgatcg
    10201 acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg accatagaag
    10261 atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt ttcatttgtc
    10321 ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca
    10381 taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg atcgcacctg
    10441 tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga atacctggaa
    10501 cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc ccatgcgaac
    10561 gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat tggatgtctg
    10621 tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt cccaacgaga
    10681 tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat ctatattcat
    10741 taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc
    10801 ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca agtttagctt
    10861 tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga gcgccatcaa
    10921 ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct ttgataactt
    10981 taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag
    11041 cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg ccaaattctt
    11101 cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt aactcagcaa
    11161 cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc ttttttattt
    11221 aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac tgctttgaca
    11281 gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga tagaagtcct
    11341 tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac tttaccttcg
    11401 atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc catgattttc
    11461 gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt gaatacttcg
    11521 tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt tttgataact
    11581 ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc atcaactgtt
    11641 aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc gcttacttca
    11701 tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt tcacctcctg
    11761 aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag ttactttgct
    11821 caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc gctttgctcg
    11881 aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc acttcgttca
    11941 tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta ttgaatatat
    12001 tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct tcggtacaag
    12061 ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga attatttttc
    12121 agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa catggatacc
    12181 aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag ctgctgatgg
    12241 tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt tttcatcgtc
    12301 aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac ggtaagttac
    12361 tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg taccagtttt
    12421 agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta cagtagccat
    12481 catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac
    12541 catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg ctttgtctcc
    12601 tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata caacagcacc
    12661 acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt
    12721 tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg caacaggata
    12781 aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct ttttagccat
    12841 tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc tatatctttc
    12901 aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct acagtgatcc
    12961 ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta
    13021 ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact ttcgcaactt
    13081 tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc aatagaacag
    13141 cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca gaaataacaa
    13201 ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt gtgaatccca
    13261 tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga gttgctttac
    13321 ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga catacctcct
    13381 taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt tatctgatct
    13441 gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat agcaaattta
    13501 tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa acgctaaatt
    13561 tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac acttgcgcta
    13621 gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt gtacaaagat
    13681 tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa gtgtctttga
    13741 tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag ctgctagaat
    13801 cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata tttatctata
    13861 tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag ttggcggcct
    13921 gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg catatgaagc
    13981 cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa
    14041 gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga tcggcggtgg
    14101 caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg gtactcctac
    14161 cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta gtatactttc
    14221 tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc
    14281 cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa gactcaaaca
    14341 gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa tgtttttatc
    14401 gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat tgatgattgc
    14461 agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca tggaaaaaaa
    14521 cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct ccgtcacagg
    14581 aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag actttttaag
    14641 tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa
    14701 aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct
    14761 gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat tcttatttac
    14821 cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg cagaaggggg
    14881 atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa
    14941 aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa atatgatggg
    15001 actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt
    15061 aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg aaagtgtcat
    15121 tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt
    15181 caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac ttgcggaaga
    15241 gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga gattagttat
    15301 cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt tattaatcca
    15361 tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg
    15421 acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca cctagattca
    15481 acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc agagctttga
    15541 agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt ttgttgacta
    15601 cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt tgacgtccac
    15661 catgattgga tacccagata ccatctgccc ctgcagagat agcgattact gcatcttctg
    15721 gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt gttttgatgt
    15781 catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct tttccttcgc
    15841 cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt aatggaaatt
    15901 ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct gccgttaaaa
    15961 tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg aatctgtcgt
    16021 ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt
    16081 tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca gctactcctt
    16141 tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga
    16201 taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct gagtcgattc
    16261 cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt tctttcatcg
    16321 tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt
    16381 ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct tcattacttg
    16441 tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa tcatactaaa
    16501 cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc tttccaaata
    16561 aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg agaggcattt
    16621 tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa cgcatagacc
    16681 gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc tagttgacca
    16741 gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg gtaatcttga
    16801 tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga ctgcgcacta
    16861 tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac ttgtggcaat
    16921 gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc catggaatgc
    16981 cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag acaagttttg
    17041 atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac ttggaccatc
    17101 ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc tgttcctact
    17161 gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa catcccttta
    17221 aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct
    17281 atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt atttatcttc
    17341 gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca
    17401 cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat atagaaaaaa
    17461 gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt aatcgatttt
    17521 atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat cgatgttgac
    17581 tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc
    17641 gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt gtcgcaggct
    17701 cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg ctggtttgct
    17761 tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga caacatacca
    17821 tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt tatgaacgat
    17881 ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa cgacacggaa
    17941 aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt ttactttgtt
    18001 ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt ccattgaagg
    18061 ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc
    18121 attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag ccaaataagc
    18181 tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa gtttcgatga
    18241 ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt ttgatttcat
    18301 cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt atttcgtttc
    18361 tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca aacgttcgcc
    18421 gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg aacaagcgag
    18481 tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga cttatacttc
    18541 ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc tattgttttg
    18601 gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt ggaggtatag
    18661 cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt acagagacta
    18721 gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt gtgccgatct
    18781 tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc tatcgtcata
    18841 aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac gctacaaagt
    18901 tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag cttccttata
    18961 ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt acgttgctta
    19021 atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca attgcttcca
    19081 caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac agcagcattg
    19141 ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc
    19201 taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta gagaaaaaga
    19261 attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa
    19321 aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac gcttttcaaa
    19381 atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga cgataaactc
    19441 acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg gaactaacat
    19501 ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt
    19561 tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca atgtttggcc
    19621 gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga cgatgacccc
    19681 gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca aactttctga
    19741 aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta acgctagata
    19801 aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc ttttcgtttt
    19861 ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg atgaaccgcg
    19921 gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc cgtcttatct
    19981 gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg
    20041 tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag taggatctgc
    20101 aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat ttgagcaacc
    20161 tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc cgaaccttgg
    20221 aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt
    20281 cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa
    20341 cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta
    20401 ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa
    20461 tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga aatggctata
    20521 aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt
    20581 atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga
    20641 ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag
    20701 gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc
    20761 cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca ggccttccaa
    20821 aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca
    20881 taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta
    20941 taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga
    21001 acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc
    21061 aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat
    21121 ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg
    21181 ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa
    21241 tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc
    21301 actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg
    21361 gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt
    21421 ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa
    21481 aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc
    21541 aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca
    21601 ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat
    21661 tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga
    21721 atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga
    21781 taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta
    21841 ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta
    21901 aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa
    21961 gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat
    22021 tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa
    22081 atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga
    22141 aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct
    22201 caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca tcgattgttt
    22261 tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa
    22321 ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc tttcctactt
    22381 cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa
    22441 gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat
    22501 tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt
    22561 tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa
    22621 caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa
    22681 tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa
    22741 aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag
    22801 aaataaagtt t
//
LOCUS       DAHUWM010000021        22696 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHUWM010000021 DAHUWM010000000
VERSION     DAHUWM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.021"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(32..2413)
                     /locus_tag="NK788_000579"
     CDS             complement(32..2413)
                     /locus_tag="NK788_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCH6768930.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2403..3050)
                     /gene="recU"
                     /locus_tag="NK788_000580"
     CDS             complement(2403..3050)
                     /gene="recU"
                     /locus_tag="NK788_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCH6768931.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3114..3653
                     /locus_tag="NK788_000581"
     CDS             3114..3653
                     /locus_tag="NK788_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCH6768932.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3743..4162
                     /gene="gpsB"
                     /locus_tag="NK788_000582"
     CDS             3743..4162
                     /gene="gpsB"
                     /locus_tag="NK788_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCH6768933.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4184..4551
                     /gene="rnpB"
                     /locus_tag="NK788_000583"
     ncRNA           4184..4551
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NK788_000583"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4760..5926
                     /locus_tag="NK788_000584"
     CDS             4760..5926
                     /locus_tag="NK788_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCH6768934.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5972..7468
                     /locus_tag="NK788_000585"
     CDS             5972..7468
                     /locus_tag="NK788_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCH6768935.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7549..8232
                     /locus_tag="NK788_000586"
     CDS             7549..8232
                     /locus_tag="NK788_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6768936.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8877..10067
                     /locus_tag="NK788_000587"
     CDS             8877..10067
                     /locus_tag="NK788_000587"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCH6768937.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10169..11122
                     /locus_tag="NK788_000588"
     CDS             10169..11122
                     /locus_tag="NK788_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCH6768938.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11191..12540
                     /locus_tag="NK788_000589"
     CDS             11191..12540
                     /locus_tag="NK788_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6768939.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12723..13046)
                     /locus_tag="NK788_000590"
     CDS             complement(12723..13046)
                     /locus_tag="NK788_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768940.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13175..14338
                     /locus_tag="NK788_000591"
     CDS             13175..14338
                     /locus_tag="NK788_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCH6768941.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14408..15757
                     /locus_tag="NK788_000592"
     CDS             14408..15757
                     /locus_tag="NK788_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCH6768942.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15802..16233)
                     /locus_tag="NK788_000593"
     CDS             complement(15802..16233)
                     /locus_tag="NK788_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6768943.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16621..19263
                     /gene="alaS"
                     /locus_tag="NK788_000594"
     CDS             16621..19263
                     /gene="alaS"
                     /locus_tag="NK788_000594"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCH6768944.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19463..20059
                     /locus_tag="NK788_000595"
     CDS             19463..20059
                     /locus_tag="NK788_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6768945.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20181..21329
                     /locus_tag="NK788_000596"
     CDS             20181..21329
                     /locus_tag="NK788_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCH6768946.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21367..21780)
                     /locus_tag="NK788_000597"
     CDS             complement(21367..21780)
                     /locus_tag="NK788_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCH6768947.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21859..22290
                     /locus_tag="NK788_000598"
     CDS             21859..22290
                     /locus_tag="NK788_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCH6768948.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22389..22622)
                     /locus_tag="NK788_000599"
     CDS             complement(22389..22622)
                     /locus_tag="NK788_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCH6768949.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         7288 a         4011 c         4759 g         6638 t
ORIGIN      
        1 aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct gaggtgctgc
       61 tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca ctggaagaag
      121 caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg
      181 ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac tcagcagttg
      241 attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt gagcttccag
      301 gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca ttcgatctag
      361 ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg cttggcatct
      421 cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga taaacatcag
      481 aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa cctgtccaca
      541 cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac accccagaag
      601 aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc ttaccggctt
      661 gatataaccc agcgatttgc gcattcgtac cagttccttc agtgatcgta tcttttaaaa
      721 tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct ggctcataag
      781 tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg taataagtcc
      841 ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct ccgtattttg
      901 tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc gtactgtatt
      961 cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca ttgaataatt
     1021 tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg gtgcccatat
     1081 attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac ggtgcgtcag
     1141 taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa tctgtgacag
     1201 gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg cctaaagtta
     1261 cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta ttggtgtcga
     1321 taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga tccgtgttga
     1381 cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt
     1441 ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct ttgacataat
     1501 tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca tccgttaccg
     1561 gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga agcatcgtat
     1621 acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga tcataggcgg
     1681 aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt
     1741 tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt gacatataca
     1801 ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc aatcttacag
     1861 ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg gtggagaaga
     1921 aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa
     1981 agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt ttataaaaac
     2041 ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa
     2101 ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt gtatatagtt
     2161 tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct tttgcgtaat
     2221 accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt attcctaaaa
     2281 ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa cctttgccag
     2341 aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag cttctagttt
     2401 gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata gaagtcaacc
     2461 gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc tagttcttcg
     2521 atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc
     2581 aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa caagacaaag
     2641 cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc atggaagttt
     2701 ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa gtaaaagcct
     2761 cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc cttgatcaca
     2821 gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg ctttttatgg
     2881 ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc ttcttcaaag
     2941 cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat tttttcgacg
     3001 ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat ctttaatcac
     3061 actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct gagatggata
     3121 agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt tttaaagaaa
     3181 atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat cagttagcag
     3241 aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc
     3301 aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt ttgtatcctt
     3361 tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac
     3421 aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct agtcagctaa
     3481 agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc tatgatgaag
     3541 aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa gcagatcatc
     3601 cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa taatcgatat
     3661 tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg ttattacgta
     3721 aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag acattttaca
     3781 acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg aattcttaga
     3841 caatatcatc aaagattatg aagcttacag caaagaactc cttgctttac aagaagaaaa
     3901 cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag ctcaaacacg
     3961 cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata tcttaaaacg
     4021 cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag caagtgcagt
     4081 caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct tagatgataa
     4141 tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg ggtaatcgcg
     4201 gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt
     4261 tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg aaaacaagct
     4321 aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc ggctggggaa
     4381 acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat ggtaggggcg
     4441 ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata gatggttacc
     4501 gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga aaaccgcaaa
     4561 catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa aaacatttga
     4621 aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact tttgtatcac
     4681 cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc tccttttttt
     4741 ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg caactgcggc
     4801 aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg aatgccaagt
     4861 tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag ctaatttatg
     4921 gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga
     4981 tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac tagatgcagc
     5041 ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg attgccaagc
     5101 gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt
     5161 accactgacg gaaactggcg cacattttca gttggaagta gctttgctga aagaccatgt
     5221 gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc gcttagaaaa
     5281 aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga caaattggcg
     5341 aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta ttgaagcagc
     5401 cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg aatcttggga
     5461 ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa aagcagatta
     5521 tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg aaattgccaa
     5581 agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac aagcagtcaa
     5641 agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt acggagaacg
     5701 tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg tattccgtcc
     5761 attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg aattttacgg
     5821 acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg atctattcca
     5881 gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt actaattgat
     5941 tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt tacaagaagt
     6001 aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata cacaaacagg
     6061 tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt acagcattta
     6121 tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt ctgaacatcc
     6181 ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag agtatgattt
     6241 ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt cttcagcaca
     6301 cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac acgcattgaa
     6361 tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg aaaagacgaa
     6421 ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc ttgataaggt
     6481 atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag aaaaagggac
     6541 tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga agagatttgt
     6601 agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag atgacactgt
     6661 ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac gatggaacga
     6721 aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg gtatgtatga
     6781 acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat caatgggtat
     6841 ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca gcttctggaa
     6901 aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata ttccttttga
     6961 caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg aggcagacag
     7021 tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga
     7081 aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag agtatctggg
     7141 aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt caggtgcaag
     7201 ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat tacttcattc
     7261 tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt ctccaatcaa
     7321 aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa atcaattaat
     7381 catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata tgaaagacct
     7441 gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct gaaattcatc
     7501 taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat ggataaatta
     7561 caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg acatgggagc
     7621 gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga accgcaggca
     7681 gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga taaagtcgtt
     7741 aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt gccaatagta
     7801 gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc taaaaatctc
     7861 tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga tcgtatcgaa
     7921 gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca tgggcacccg
     7981 atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc
     8041 acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact
     8101 gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa
     8161 ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg gatcaaaaat
     8221 aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt cggatcattt
     8281 tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg ccgttcagct
     8341 ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat attgtaactt
     8401 gtgattcgga attggcagga gaacaccgtt tattcggaag aataggactg tgacaagact
     8461 tttgtcacag tccttttttc gaattcaaca agtaaaactt ttaagaaata ttcagagagt
     8521 aaaattaaaa attgctgata aacgaagatg gaaattttat gtatttttat tggtctatgc
     8581 aataaataaa ttaaatattc agaatacaat tgattttaac gaaagggctt ctatcaatgg
     8641 ttgtgttgta tttatctgcc caggtagagt aaaatagatc attatctaaa aggtaattgt
     8701 tcatactata taagtcattt gaaagtaaaa agatactgta aataaaaaat gtggttaaat
     8761 aattctcata atcagtgtaa gcgttatcaa aaatatagca tacttgcttt gtagatgaat
     8821 gacacgatat atgtttggta tgttctgcaa gacagatgaa tatggaggtg gagacaatgt
     8881 cttccaaaga actaacaatg tatttagaga aagaattgga tcaactgaaa gaaaaggggt
     8941 tatacaatac gatcgacata ctagaaagtg aaaatggtgc ttgtatcatc gtagacggaa
     9001 aaaagatgat caatctagca tccaacaatt atcttggatt tgccaatcgc gaagaattga
     9061 aaaaagcatg tattgaagca accgaaactt atggtgtagg agcaggagca gtccgaacga
     9121 tcaatggttc gctgaaaatc catcagcaat tagaagaaaa aatcgcagaa ttcaaaggga
     9181 cagaagcagc aatcgccttt caatctggat tcaactgcaa tatgggcgct atctctgcag
     9241 taatgacaaa agaagatgct atcttatcgg atgaattaaa ccatgcatct attattgacg
     9301 gttgccgctt atccggagca aaaatcatcc gaattaaaca tcaagatatg aaagaccttg
     9361 agaaaaaagc aaaggaagcg atagaaagta agaaatataa aaaaatcatg tatatcacag
     9421 atggtgtttt ctccatggat ggagacattg cacgattgcc ggaaattatt ccgattgttg
     9481 aaaaatatgg actgatcacc tacgtcgatg atgcgcacgg ctcaggcgta actggaaaag
     9541 gtgcaggaac cgtcaagcat tttggattaa gcgacaaaat cgatatgcaa atgggcacgt
     9601 tatcgaaagc tataggtgtt gttgggggtt atgtagctgg atcgaaaaca ctaatcgact
     9661 ggttgaaagc tagaagtcgg ccattcctat tttctacttc gctaactcca ggtgcagctg
     9721 cagctgcatt ggcttcgatt actttgatgc aagaacatcc ggaacttgtc gaaaaagttt
     9781 gggaaaatgc taattatttt aaagaagaat tgaaaaaagt aggctataac atcggtatgt
     9841 ctgaaacacc tattactcca gtcattttgg gagacgaaaa agtgactcag actttctcta
     9901 agaagctgat cgaacatggg atttatgcta aaccaatcgt ttatccaact gtgcctcttg
     9961 ggacaggcag gatcagaaat atgccgactg cggaacatac gagggaaatg cttgatgaag
    10021 cagtagcagt ttatcaaaaa ataggaaaag aaatggaaat tatttaacta aatttagttt
    10081 tttcgtctat ttaaaattag agaacatgtt cacgcatata ttgttaatca tctgaagata
    10141 gtagtaaaaa agatcaggga ggatcatcat gaaaaaagta ttggtgacag gatgtttagg
    10201 acaaattggt tcagagttga tcacacggct acgtcaagaa ctaggcagtg aaaatgtcat
    10261 tgcgacagat atacgcatgc ctgaagtcat tgagaaaaac gatatctttg agatcctaga
    10321 tgtaatggat tacgaaaaaa tgcgcagcat agcagaacaa tatcaagtgg acacattgat
    10381 tcatttggct gctcttttgt cagctgttgc agaagcaaaa ccacaattag cctggaattt
    10441 gaacgtgact gggctggtca atgcattgga agtcgctaga gaattccagc tgaaattttt
    10501 tacgccaagt tcaatcggcg cgtttggtcc aaatactcca aaagatcata ccccacaaga
    10561 tactcttcag cgtcctacaa ccatgtatgg ggtaacgaag gtagctggag aactactatg
    10621 tgattattat tatgtgaaat atggcgtaga tactcgtggt gtccgtttcc ctggattgat
    10681 ttcttatgca accttgccag gaggcggtac gacagactac gcagtcgata tttattattc
    10741 agcaatcaaa gaaggaaagt acactagttt cattgatcaa ggtacatcaa tggatatgat
    10801 gtatatgcca gatgcgatcg atgccattat aaaactcatg aatgcagatc ctgatcgatt
    10861 ggttcatcga aatgccttca atatatcggc gatgtctttt gacccggaag agatcaaagc
    10921 atctattcaa aaaatcatac cagattttga aatggattat gatgtggatc ctgtacgtca
    10981 gtcaatagca gaatcttggc caaattcact agatgtttct tgtgcgcaag aggaatgggg
    11041 cttttcgccg aaatacgact tagatgtgat gacaaaggat atgttggaaa aactgaaaga
    11101 aaaattggta gagacggtat aaaacaagaa ccacatccaa aaagtttttt atacttttgg
    11161 gattggctat ggtatgaaag gggacaaggg atggaagaat atcaagccac gccagtaaaa
    11221 gaagtaaaaa tagaaaatga attaaaacgt acgatgggat tttttactgc tttatcgact
    11281 gttatgggca cagtgatcgg tgcaggggta ttttttaaag ccgcaagcgt agcagaagtt
    11341 acaggatccg ccagtttgca tatgttttct tggtttctcg gtggtatgat ttctgtctgt
    11401 gcaggactaa caggagctga actggcagct gctattccag aaacaggagg gatgattaaa
    11461 tatatcgaac ggatttacgg aaatacagca gcttttttat tgggctgggc gcaagtcgtg
    11521 atttattttc cagcaaatgt agcagccttg tcgatcattt tcggtacaca gttcgtcaat
    11581 ctatttggtc tttcccagtc aatgattgtt cccgtagctg ttacagctgc tgtatcgatc
    11641 ctgttgatca attttttagg ttctaaagct ggaggggctt ttcagtcgat tacgcttgtc
    11701 tgcaagctta ttcctttgtt tgtgatcgtc attttcggtt tgttccgaca agaaggagta
    11761 gacttccaac tgtttccgat ccaagcagga gaaaatcttt catttttctc agccttggga
    11821 gcgggcttgc tagcaacgat gttcgcatac gacggttgga tacacgtagg aaatatttcg
    11881 ggagaattga aaaagccggc gaaagatttg ccaaaagcaa tttctttagg aatcatcgga
    11941 atcatgatcg tttatttatt agtaaatgcg gtgttcttaa gaaccgcatc tatcgacgga
    12001 gtcgcaggaa acagcaatgc ggcaagtgac gttgcaaaaa tgatttttgg cggcttcggt
    12061 ggaagactgg tcacagttgg tatcttgatt tctgtttatg gaaccatcaa tggctatacc
    12121 cttactggaa tgcgtctccc ttatgttatg gcaaaagaaa acaacctgcc ttttagcaag
    12181 ctctttgcta aacttcatga taaaactaaa gttcctgtag ccgcaggtat cttagagcta
    12241 gtcatcgcta ttggcatgat gatgatcggt ggttttgata cactaacgga tatgctgatc
    12301 tttgtcattt ggatttttta tacgatggtc tttgttggtg tcattcttct tcgtaaaaaa
    12361 gaacctgatt tgtttcgacc atacaaagtt ccaatgtatc cgtttattcc actagtagca
    12421 atcattggag ggacttttat cgtatcaagc acgcttatca ctcagacctt tttagcttcg
    12481 atggggatag tattgacact tgctggtatc ccgatttatc tctatttaaa acgtagatag
    12541 ctaaaacatt cgaattttat tagaactgca cttctctgcc aaacaaaata ttttatttaa
    12601 atgaggagta cgtggaagaa atgtcaaaag tactcgttaa ttttgatcac gtaaatgtcg
    12661 ttttgtctac gatgaaatag caaagtaaaa gacagaaatc gggctcaacc gatttctgtc
    12721 ttttactttg atttgcgtaa ctgaactatt cggcgtacgc cgcgataaat atttaataaa
    12781 accaagcaac cagaaatgaa aatgggaagc caaatcagat agacagggta gccaagcact
    12841 ttagacaaag catgtggagt cattttatcc atttcaagaa gtgcttgctg ccaagtctga
    12901 agattgaaga ctagcatgaa acccgaaata atcaggagaa taaatcctaa ataatgaatt
    12961 gtagttcttt ttcttacata agcgatcgta gcataaagga acagtaaaaa aataggtacg
    13021 ccaaaaacaa agtacatatt cagcatctcg acaccccctt tgtgtttatc ttactttact
    13081 ttgaaagaaa tgagaaataa tagcctcgtt acttgcttga aacagcaaag gactgctaag
    13141 ataagagaga tacaaagaat caaggaggag tcatatggca agttatatcg aaccgattcg
    13201 ttttgcgctg attgtttttc catttttagc cttagcgatc tcatctgttt tttttattta
    13261 tgaatatcga aagtatggaa catttctagt aacaagggca gttattttgt attctttcgt
    13321 tttttattta ttgtgtgcgt attttttggt gatcttacca cttccggcaa aggagacggt
    13381 tgcccaaatg acagggccaa agatggagct gcatttattt gcttcatggc aacatttcag
    13441 agacaacacg gtattggtct tgtctgaacc tagtacctac ctgccggcaa tgaagcagag
    13501 tgtatttttg gaaccagctt ttaatgtttt attactcata ccatttggca tttatttaag
    13561 atattatttc cggctttctt ttatgaaaac gatgattgct agtttctgcc tttctctatt
    13621 ttttgaattg acgcagctaa gtggacttta ttttatctac ccaagaccat accgcttatt
    13681 tgatgtgaat gatttagtga caaatacatt gggtggaatc attggttttg tcgttacgcc
    13741 gttatttaca tttatgcttc ctacaagaag acgaatggat gaactttctt atgaaaaagg
    13801 aaagactgtc tcattgactc gacgcttggt cgcttatttg atcgactgga catttttatc
    13861 tcttattaat ttggtagcgg cgattttctt taaaattgcg ttcgattcag gggcagttac
    13921 ttctcatatc tggtggcttt tattagaagt aggggtatac tttatgattg taccgtttat
    13981 aaccaatgga tggacacttg gcaaaaaaat tgtgcgtatc cgtttggtgg aagaagaccg
    14041 cgaacgaatt tctttaaaag cattgatgat ccgctacggg tatctgtact ttctttttta
    14101 tggtttaagc tggttggggc agttgcttca gcaaatcatg aattcgacaa cgaatacggt
    14161 gttgcagatg agtgcagtat tcatttctct cggtgtcttg ttactacaag gtatttttat
    14221 tttaaatatc ttgctatcat ttttgagaaa aaaacggatt ctgttttatg aaaaagctag
    14281 tcacacatat actgttagca cgattggcca aacaaaggat gatgattctc gaaattagct
    14341 gagcaaaaac gctttgtctg tgttataatg ccaaagtcgt gataatttaa agagaagagg
    14401 tgcacgaatg aataattttg atatattccg cttcaaaccg tttatcaatg aagcgttaac
    14461 cgcaaagggc ttcaaacagc ccacagaggt ccaggaacgt ttgattcctt taattgcaaa
    14521 aggaaaaaat atcattgggc aatctcaaac tggtacagga aaaacacata cgtttttgtt
    14581 gcctttgttt gaaaaaatca atgcagaaaa agaggaagta caagttgtta ttacagctcc
    14641 aagtagggaa ctagctgcac agatctatga agcagctaca caaatcgcta aattttctac
    14701 accggagatc cgtgtctcga attttgtcgg cggtacagac aaacagcgtc aattaaatcg
    14761 tctgcatcac caacagccac aggttgtagt aggcacgcct ggtagaatat tagacatgat
    14821 gaatgaacaa gcgctgaaga ttcacaccgc atttgcattt gttgtcgatg aagcggatat
    14881 gacattggat atgggttttt taaatgaagt agaccaaatt gcaagtcgat taccagaaaa
    14941 gctgcagttt ttagtttttt ctgccactat tccagaaaaa cttcgtccat tcttacgaaa
    15001 atacatggaa aatccaatca ttgaagaaat caaaccaaaa gcagttattt ctgaagacat
    15061 tgaaaattgg ctgatttcaa caaaaggtaa agatgagaat caaatgattt atcaactgct
    15121 gacaattggt catccttatt tagcaatcgt atttgccaat acaaaacagc atgtggatga
    15181 aattgccgat tacttgaaaa accaaggctt gaaagtagca aagattcatg gagacatcac
    15241 cccaagagag agaaaacgag tgatgcgaca agtacagaat ctggagtatc aatatgttgt
    15301 agcgactgat ttagctgcta gagggatcga tatcgaaggt gtctctcatg ttattaatgc
    15361 agaaattcca gaagatctag acttctttat ccatcgtgtc ggacgtactg gacgtaatgg
    15421 cttaaaagga acggccatta cgttatattc gccaaatgat gagcaagcca ttgatgcgat
    15481 agaaaaattg ggcgtctcat ttgaaccaaa agaaatcaaa aacggggaaa tcgtcaaaac
    15541 atacgatcgt aatagacgtg ccaaacgaga aaaatcaaaa gatgtattag atcccacgct
    15601 gattggtctt gtgaagaaga aaaagaaaaa gatcaagcct ggttataaga aaaaaatcga
    15661 ttgggcaatt gcagaaaaaa ataaaaaaga acgcaaaatc gaacgcagac agcaaagccg
    15721 tactgctcgt aaaaataaga aaaattcaca taaataaaaa acaagaggtt gtgacaaaag
    15781 gcgtgtcaca atctcttgtt tttaagctag actttttttc attccgatac aaagtacttc
    15841 atcttcaata aaaggttctg tgcaagtatg atacccttgt ttttcataaa aatctacagc
    15901 cgtcatttca gctactacat aactagtctg acaaccttct gaaaccgccc gtttttcagc
    15961 agcttcaagc aactgaccgc ctaacccttt tcctcggtat tcttttgcta cgcaaaatcg
    16021 ttccggcttg aagcaagtag gtgttttctt ttcataacgc aaagtagcta ctggtctttt
    16081 ttgatcaaga aatagataat aggtttgttg cgggatatct tttccgtcga attctagttc
    16141 tgcaggaatc ccttgttcta atacaaaaac ttgatatctc aaaaagcagc tagctgcgcg
    16201 tatccattca tccgctccaa ctattttttt catcatattc ctccttgtca tgaatagcag
    16261 agatgtattg attcctttat tcttatcgta ccaaaaatcg tggagtatga ccaaaaggaa
    16321 atgaaaataa aaattgagat attgacagtg atcaatagaa tgcgtatact aattaagtac
    16381 gaaaaggata tgatttgttt tttaagactc acagaaaatt ctttcttggc tgaaaaaaga
    16441 atagtcacta atcaaattgc tccctttttc tattttggta gaaataccga cgcagctcag
    16501 cgttatcgag tttaagaggt aatgattaca catcattgca atcaaggtgg taccgcgagt
    16561 tttcgtcctt ggctgtgatg gtgttttttt gtatttaatt tatagaaagc aggtaatgac
    16621 atgaaacaat taagtagtgc acaagttcga caaatgtttc ttgatttctt ccaatctaaa
    16681 ggacatacga ttgaacctag tgcgtctttg atccccgtga acgatccaac cttattgtgg
    16741 atcaactctg gtgttgcaac attgaaaaaa tattttgatg gttcagttgt gccagaaaat
    16801 ccaagaatca caaatgccca aaaatctatt cgaacaaatg atatcgaaaa tgtcggtaaa
    16861 acagcacggc accatacgat gtttgagatg ctgggtaatt tttcgattgg tgactatttc
    16921 aaaaatgaag ccatccactg ggcatgggaa tttttgactg atccaaaatg gatggcattc
    16981 gatccagaaa aactttatgt aacagtttat ccaaaagata cagaagcaaa acgcatctgg
    17041 catgaagaag ttggcctgcc actagatcac attattgatg tagaagacaa cttctgggat
    17101 atcggtgcag gcccatgtgg tccggatacg gaaattttct atgaccgtgg agaagcttat
    17161 aacgatttag cagaagacga tcctgaaaac tatccaggag gagaaaatga acgttattta
    17221 gaaatctgga acttagtatt ttcagaattc aaccataagc ctgataacac atttgaacca
    17281 cttcctcata aaaacattga tacggggatg ggattggaac gcatggtctc aatcgttcaa
    17341 gatgcgtcaa cgaactttga gacagacttg tttatgccta ttatccacgc cgtagaaaag
    17401 ctaagcaaac aagtaaaata tggtgaaaac ccagtaacag atatttctta caaagtaatc
    17461 gcagatcata ttcgcgccct ttcatttgct attggcgacg gtgcattgcc atcgaatgaa
    17521 gggagagggt atgtcttacg ccgtttgctt cgtcgtgctg tcatgcatgg gaaaaaatta
    17581 ggtattgaac aagcattctt atatcgttta gtccctgttg ttggggaaat catggtaagt
    17641 tattatcctg aagtacttca acaaaaagaa tttattgaaa aagttatccg tacagaagaa
    17701 gaacgtttca atgaaacaat caatgaaggt ttggaaatct taaatcaagt gatccaagaa
    17761 gtaaaaaacg aaaacgggac gactctagca ggaaaagaca tcttcaaact atacgacaca
    17821 tatggcttcc cggtagaatt aacggaagag gtggcagaag atgaaggact gaaagttgat
    17881 catgctggtt ttgaaaaaga aatggatgca caacgcgaac gtgctcgctc tgcccgcagt
    17941 actgaaaaat caatgggggt acaatctgct ttattgacag atatcaaagt agaaagtaaa
    18001 ttcattggat acacagaaca agaatcagat agtcaattat tggtcatcgt taaaggtgag
    18061 tctcttttga atgaagtcgc agaaggtgaa gctcaaatga tctttgatca aacacctttc
    18121 tatgcagaaa tgggtgggca agtagcggat caaggatgga tcatgaatga agtaggcgaa
    18181 atcgtagcag ttgttacaga tgttcaaaaa gcgcctaatg gtcaatttat gcacaccgta
    18241 gaagtaaaag caccaatgag tgaaggacaa acctatcatc tattgatcga tcaatcgatg
    18301 cgcaatcgga tcatcaaaaa ccatacagca acccatctac tgcacaaggc attgaaagaa
    18361 gtacttggcg agcatgcaaa tcaagctggt tctttagtcg ctcctggaca tttacgattc
    18421 gactttaccc actttggtca aatcacttca gatgaattag ctgaaatgga aagaatcgtc
    18481 aacgaaaaaa tctgggaagc tatcccagtt gaaacaattg aaacagatat cgatacagcc
    18541 aaaaacatgg gtgcaatggc tttgtttggt gaaaaatacg gacatgacgt tcgagtagta
    18601 aatattggcg gctggtcaat cgaactgtgc ggaggcacac atgtagcaaa tacagaaaac
    18661 atcggaatct tcaaaatcgt ttctgaatca ggaattggtg caggcgtgcg acgaatcgag
    18721 gctgtaacaa gtaaagaagc gtacgaacta cttgaagaag aagaaaaaca actaaaacaa
    18781 gtagcaacaa ttgttaaatc acctcaattg aaagaagttg tttcaaaagc ggaacaattg
    18841 caacagcaag taagagagct acaaaaggaa aatgaacaat tagctagcaa gttagcgaat
    18901 caacaagctg gtgatgtatt caaagatcca gaaacgatca atggaaaaac aatcattgca
    18961 gcacaagtaa aagtgaaaga tatgaatcag ctgcgtcaat tagctgacca atggaaacaa
    19021 aaaaatattt cagatattct tgttttagca aacgaacaag aaggaaaagt cagtctgcta
    19081 gcggcaatga cgaaagaatc aaatgaagct ggactaaaag ctggcgactt gattaaagca
    19141 atcgcaccga aagtaggcgg tggcggtggt ggacgtccag acatggcaca agctggcggg
    19201 aaaaatcctg ctggtattcc agatgcactt aaagctgcga aagaatgggt tgcgcaacaa
    19261 tagggcgttt acgtttgaca gaaaaacaaa agttcaaaca ttgaacgaaa catgattatg
    19321 ggggcgaaga caaacaagtc ttgccctcat acttttttca attggatggc gcatctaatc
    19381 aaatggtcgg attcggatgg tctttagttg acgaaagtga cagaaatttt tacgattagt
    19441 ataaataaaa aggggtgaaa aaatgaatag tagaaataag acctttcgac tagtcttacg
    19501 tgcgatcttg ttagcaatca tcatcgtaca agcgatggtt ccttggctgg ggtttattcc
    19561 attaggattc attagtttaa caattatcca tatcacagta attattgctg cagtagtatt
    19621 aggcccaaaa gatggcatgg tgattggctt gttttgggga atcgctacaa ttgttcgtgc
    19681 ctatgccatg ccaacaacgc catttgatac attagtgttc acgaatccga ttatctcggt
    19741 agttcccaga gtacttgttg gcttagtagc aggcttggtt ttccactgga tttatcaaaa
    19801 aaataaatca ataacgatca gctcagcctt tgcaggggta ctgggttcat tggtcaatac
    19861 agtcttagtg ttaggattca tgggattatt ttatacaggt gcaacggcag atgcctatgg
    19921 tgtagactct gctcttttat ttaaaacctt agcaggggtt gcagcaatca atgggatacc
    19981 agaagcaata ggtgcgggaa tcataacacc attactagca aaagcgttat ttgcagcaac
    20041 accgttgaaa ccagaataat gaccatcttt taatagtaat aggacgcacg atagatagaa
    20101 aaaggaagag aggagaggaa aacaatcgtc ctctccatct ttttacctga gcaacaagca
    20161 aactagaagg ggtaaaaaac ttggctaaga aacaagaaga aaaaaagaaa caaataccgc
    20221 aacagcggac aaaaaaagga gtactagcgg gtatcttagg attggttacg aatctgatct
    20281 tgtttgtctc taagctaatg atcgggcttt tttcaggaag tgtctccatt atggctgatg
    20341 cagtgaacag tctatcagat actgcttcat ccatattgac tttgatcgga tttaaaattg
    20401 ctgcaaagcc agcagatcag gaacatcctt atggacacga acgcttcgaa tatatcagtg
    20461 ggttatttgt ttcgatcatc attacttatg taggatttca atttttagag tcttctgctg
    20521 aaaaaatcat ccatccagaa aatgtctctt taacacccat cgtctttttt gtcctaatca
    20581 tttccatctt tttgaaattt gcacaaggca aaatgtatca ggttattgcg caatccattg
    20641 attctgaaac attgagagct acagcaacag atagttataa tgatgtcttt actacactgg
    20701 ctgtattgct ttcagcagca atcgaatggg taactggatg gcgtattgat ggatatatag
    20761 gttttgcttt agcattatat atcctttata gtggattgac gatgcttcga aattttgtat
    20821 acgaactatt aggaagtcgc ccaacgccag cagagattga agaaatggaa gagcatttaa
    20881 atgcttgcga tgatatactt ggctatcatg atttattggt gcataactat ggacccaata
    20941 aacgctttgc atcagtccac atcgaagtag atgaatcaat gaatttgaat gtggctcata
    21001 agattattga tctgatagaa aaagacttta aaaagtcttt ggatgtggaa cttgtctgtc
    21061 acttggatcc aatgcctgtt gataacgaaa aatactataa tatcttgcgg cagttaaagc
    21121 aaattcttct tgaaacaggt acagggctta atattcatga ttttagagta attcatcaag
    21181 gaaaaacgct gcaatttgat gtagtggtcc ctgagaaatt ccagtttgtt gatacagaac
    21241 tagaaagaat cttacgtagt caaatagaag aaaaaatcgg agattaccaa ttggaaatta
    21301 cgtttgatca caattatctt ttatattaaa tgagaaacta taaaaagact ccttaaagtt
    21361 tttaatttaa agagtctttt tttcttttaa ttgtttatgg agagcctgac gtgcaagatt
    21421 atctgctcct ttatttttgt tttcgggcaa ccattgcagg atcaataaag aaaattcgga
    21481 tagaagttct tggattcttt gaaggtaggg aaggaagagc tcgttcttcg ttgcgttttt
    21541 gtcgaccgta gaaactagtg ttttgctgtc tgaaaaacag aaaatcgttt cattctgtaa
    21601 gtcgtttttc tttagaaatt ctaaagtttt taaaaagact tcgaactctg cttgatggtt
    21661 tgtccctata gaaagcggga tagatagctg ttcttgttta ccttgataga caatcaagat
    21721 tccacctcca ctaggaccag ggttgccttt tgtagaggca tcaatgtata cttttaacat
    21781 aaaaagagat tctccttttt gttcaaacta atgttatggt aagattatat cattactgaa
    21841 tcggtgagag gttagagaat gaaaaaaaat attcgatggc agcctgaact ggcacatacg
    21901 atcatctatt ggtcattgac cttgatcatt ctgtttttta gtctgattct ttctttagaa
    21961 aacacaggtc cttattggaa aagcaatttt gttttaggta tttttcttgt ttttctattt
    22021 ctaggtttgc ggcgttcttt tattttaaaa tcaaactctg tgattatacg ttatgctgct
    22081 ttttggaaag accgagaaat atctgtcgga cagattcgat ctttgacgcc aactaatact
    22141 ggggtgcaaa tgatgttgca agatgagaaa gccccatctg ttttcatgat gcgaaaaaaa
    22201 gatcaagtag tcctttttga tcattttgaa aaaaacggac ccaaatcaga atcaattcct
    22261 tatccttata cgaaaaaaag aaaaagctaa ttttaaaaat cagaacctaa aaaaacgaat
    22321 ttttacagga aaagaaaaga gtgagacgta cggttcatcc cgtttagcgt ctcactctat
    22381 ttgtttatct attcatgcat actagtatcg gtagtctgaa cgttgccttt cttgacatgg
    22441 gcagcttgtg ttcctcgatt cccttcgatg atctcaaatt cgaccacttg attttcttcc
    22501 aaagttttga atccctcttc ttcaatcgca gtaaaatgta caaaaatttc ttctgtgtca
    22561 tcataactga taaagccgta tccttttttg ttgtcaaacc attttacaat tcctgtggtc
    22621 aaagcactca cgacctttct aaagtctgat catagccttc attataaaga gtatttataa
    22681 gtacgtcaaa taaatt
//
LOCUS       DAHUWM010000022        21912 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHUWM010000022 DAHUWM010000000
VERSION     DAHUWM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.022"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="NK788_000600"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="NK788_000600"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCH6768950.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="NK788_000601"
     CDS             complement(1172..1867)
                     /locus_tag="NK788_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCH6768951.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="NK788_000602"
     CDS             complement(1880..2596)
                     /locus_tag="NK788_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCH6768952.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="NK788_000603"
     CDS             2926..3534
                     /locus_tag="NK788_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCH6768953.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="NK788_000604"
     CDS             3859..4182
                     /locus_tag="NK788_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCH6768954.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="NK788_000605"
     CDS             4364..5734
                     /locus_tag="NK788_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCH6768955.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="NK788_000606"
     CDS             complement(5864..7165)
                     /locus_tag="NK788_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCH6768956.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="NK788_000607"
     CDS             complement(7519..9477)
                     /locus_tag="NK788_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCH6768957.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="NK788_000608"
     CDS             complement(9654..9920)
                     /locus_tag="NK788_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6768958.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="NK788_000609"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="NK788_000609"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCH6768959.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="NK788_000610"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="NK788_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCH6768960.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="NK788_000611"
     CDS             13264..13827
                     /locus_tag="NK788_000611"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCH6768961.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="NK788_000612"
     CDS             complement(14080..14601)
                     /locus_tag="NK788_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCH6768962.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="NK788_000613"
     CDS             complement(14604..15446)
                     /locus_tag="NK788_000613"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCH6768963.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="NK788_000614"
     CDS             complement(15439..16164)
                     /locus_tag="NK788_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCH6768964.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="NK788_000615"
     CDS             16340..16636
                     /locus_tag="NK788_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6768965.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="NK788_000616"
     CDS             17204..18796
                     /locus_tag="NK788_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCH6768966.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="NK788_000617"
     CDS             18809..19549
                     /locus_tag="NK788_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6768967.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="NK788_000618"
     CDS             19717..21066
                     /locus_tag="NK788_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6768968.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="NK788_000619"
     CDS             complement(21194..21784)
                     /locus_tag="NK788_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCH6768969.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAHUWM010000023        21801 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHUWM010000023 DAHUWM010000000
VERSION     DAHUWM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.023"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..334)
                     /locus_tag="NK788_000620"
     CDS             complement(<1..334)
                     /locus_tag="NK788_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCH6768970.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYA"
     gene            505..1461
                     /locus_tag="NK788_000621"
     CDS             505..1461
                     /locus_tag="NK788_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCH6768971.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1534..1650
                     /locus_tag="NK788_000622"
     CDS             1534..1650
                     /locus_tag="NK788_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCH6768972.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1705..3387)
                     /gene="recN"
                     /locus_tag="NK788_000623"
     CDS             complement(1705..3387)
                     /gene="recN"
                     /locus_tag="NK788_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCH6768973.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3405..3854)
                     /locus_tag="NK788_000624"
     CDS             complement(3405..3854)
                     /locus_tag="NK788_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCH6768974.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3961..4779)
                     /locus_tag="NK788_000625"
     CDS             complement(3961..4779)
                     /locus_tag="NK788_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCH6768975.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4788..5678)
                     /locus_tag="NK788_000626"
     CDS             complement(4788..5678)
                     /locus_tag="NK788_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCH6768976.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5680..5904)
                     /locus_tag="NK788_000627"
     CDS             complement(5680..5904)
                     /locus_tag="NK788_000627"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCH6768977.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5909..7246)
                     /locus_tag="NK788_000628"
     CDS             complement(5909..7246)
                     /locus_tag="NK788_000628"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCH6768978.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7247..8101)
                     /locus_tag="NK788_000629"
     CDS             complement(7247..8101)
                     /locus_tag="NK788_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCH6768979.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8198..8647)
                     /gene="nusB"
                     /locus_tag="NK788_000630"
     CDS             complement(8198..8647)
                     /gene="nusB"
                     /locus_tag="NK788_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCH6768980.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8640..9065)
                     /locus_tag="NK788_000631"
     CDS             complement(8640..9065)
                     /locus_tag="NK788_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6768981.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9311..10375)
                     /locus_tag="NK788_000632"
     CDS             complement(9311..10375)
                     /locus_tag="NK788_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCH6768982.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10547..10792)
                     /locus_tag="NK788_000633"
     CDS             complement(10547..10792)
                     /locus_tag="NK788_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6768983.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10931..11305)
                     /locus_tag="NK788_000634"
     CDS             complement(10931..11305)
                     /locus_tag="NK788_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCH6768984.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11388..12302)
                     /locus_tag="NK788_000635"
     CDS             complement(11388..12302)
                     /locus_tag="NK788_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCH6768985.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12323..13126)
                     /locus_tag="NK788_000636"
     CDS             complement(12323..13126)
                     /locus_tag="NK788_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCH6768986.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13159..14148)
                     /gene="manX"
                     /locus_tag="NK788_000637"
     CDS             complement(13159..14148)
                     /gene="manX"
                     /locus_tag="NK788_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCH6768987.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14251..14742)
                     /locus_tag="NK788_000638"
     CDS             complement(14251..14742)
                     /locus_tag="NK788_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6768988.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14933..17740)
                     /locus_tag="NK788_000639"
     CDS             complement(14933..17740)
                     /locus_tag="NK788_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6768989.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18174..18467)
                     /gene="rpmA"
                     /locus_tag="NK788_000640"
     CDS             complement(18174..18467)
                     /gene="rpmA"
                     /locus_tag="NK788_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCH6768990.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18480..18824)
                     /locus_tag="NK788_000641"
     CDS             complement(18480..18824)
                     /locus_tag="NK788_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCH6768991.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18851..19159)
                     /gene="rplU"
                     /locus_tag="NK788_000642"
     CDS             complement(18851..19159)
                     /gene="rplU"
                     /locus_tag="NK788_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCH6768992.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(19179..19255)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19441..20082
                     /locus_tag="NK788_000643"
     CDS             19441..20082
                     /locus_tag="NK788_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCH6768993.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20144..21775)
                     /locus_tag="NK788_000644"
     CDS             complement(20144..21775)
                     /locus_tag="NK788_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCH6768994.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         6327 a         4820 c         3640 g         7014 t
ORIGIN      
        1 cagcgtacaa gacgattgtc aacagaaaaa ccaaacgcca agtcatcttg aaataacgcg
       61 gataaataat ttcttcataa taatatgctt ggaaaacagc aatacaaatt cccattagca
      121 aaacagaaat aaagaaaaac ggtaagatag aaattgtccc tgtatttcta gacaatgcat
      181 gcataccgcc ccacaaaaaa ggaatcgcca aatcaggggc ttttatttta aaacgacgat
      241 ttaacgaaaa taattttaca agaaattggc aaccaaacaa aacgattacc gggtaaatat
      301 accaaaacaa gataagtggt gtgaatgtgc ccatgaacgt aaactccttt tttatcgttg
      361 cttcaagtat actgtatata ctttacacaa ctgtttcatt ttatttaaat ttatgcagtt
      421 atattctata tcttacaaac acattccatg tattccaatt ccctatccgt tataataaaa
      481 gaaacaggca acaggaggaa aatcatgatc aaatatttta cattagataa tgagaaactg
      541 cgtaataccg gaaaggaaac tgaccaaacg atctggtatt gtgtagaacg tcctaatcaa
      601 gaagagattc atcagctgac acatcaattt gaaattccgt cagattacat cacttccata
      661 ttggacgatg cagaaaattc ccgtacagaa gaattcaatc aatcaaagtt gacaaagcca
      721 gcgttattat tgttacaata tccttttgct aaaacaagtc ctagcggtta ttttcaagtg
      781 gatacatacc cgctctctat tattttgacg cccaaaaaga agcttatcac aattacgaat
      841 catgaaccac tgtttttaaa gaaagtcttt agccagacct ttccaaaaag tgatatttcg
      901 cctaatctca atatcttctt tcaattcatt tggcaggtca cactctctta caacagttat
      961 ttagctacgc ttcaatctca atgcgatact ttggaagcca aacttcaagt atccactgaa
     1021 aacaaacaac tttatcaaat catggatatc caaaaaagcc tcgtttattt cagagaagcg
     1081 acttctgcta atttagatgc attgaaagaa ctagcgcaaa ataaactttt ccaagaaaat
     1141 catgctttga aaaatcattt acgtgatgta ttggtggaaa cagagcaggc aatgactact
     1201 tctcgcattc aattaaaact agtagaacaa atgaatcaga ctttttcagc aatcgtatcg
     1261 aataatctga ataatattat gaaaatttta acttctttaa cgattatttt aactataccg
     1321 acaatcgtcg gcagtatcta tggaatgaac attaagttac ctattgctga aagggatgat
     1381 gcctttatat ggctagttct attaacatta tttatttgta tcgtaactac ctattattta
     1441 aaaaaaggga agtttttata aaaaagccct cttgtggaaa tggaaaaaag attgtacact
     1501 tgaatcaact aattaatgac gaggttgaaa acaatgcgtg aaaaaaaaca aacaagtact
     1561 tttgctaaag taacaaaatt tgtcatttgg acaatgctga ttttaacgat tggatctgtt
     1621 gtattgactg ctgtaatgag tgtcatgtaa aaataaaata tagctatcag ctgctgaaaa
     1681 ggacctgaac cactgcgagg tggctcaggt cctttttgcg agtttcaata gttcttttgc
     1741 atgttcgagt gtgagttttg taatctcact tccagccagc atacgtgcaa tttcttcttc
     1801 tctttctttt gttttcaact ctgaaacaga agtttgcgta cgtccatcaa tcactgcttt
     1861 cacaatataa tattgatagt cggcgactgc agcaacttga ggcagatgag tgatgcacaa
     1921 aacttgagag tatttcgata tttgagaaat tttatctgcg atggcctgtg caactcttcc
     1981 gctcacacct gtatccactt catcaaaaat aatacttgtc acaccttgct cagaagaaaa
     2041 aatagttttc aaagcaagca acatccgaga cagttcgcca ccagaagcga ctttcaccaa
     2101 aggcttcagt ggctctcctg gattggtcgt aatgtaaaat tcgatttggt caaatccatc
     2161 atgattcaat tgtctatttt cttcttttaa gaaacgtacc tcaaactcag tgttttccat
     2221 atacagactt ttcaattcat gcagaatgga tttttccaat tcttttgcga tttttttacg
     2281 cgcttcatga agatctgcgg cttgttgctg aagtagaagc tctttttgct ctagttcctc
     2341 tttcattttg tccaattgac cttctgaaaa atcagaagaa gctagctctt cggtgatttc
     2401 gtcatagtaa gccaatattg actcaatgga ttcgccgtat ttacgtttca actgtcggat
     2461 taattctaaa cgttgcgtaa cttcttctaa acgactctca tctaactcca ataaatcaat
     2521 ctgacgactc atatcgccga ttgcatcttg cagcaaatag taagcacttt ggatattttc
     2581 ataaattgct tcatactcca catctagatg ggcgatccct tgcagttctg aaacggccaa
     2641 gccaacacca tctacactgt tttgttcatt attcgtcagt gctccgtaac ctgcagctaa
     2701 tccatcagca attttttgat aattgctaag tttttctctt tcttctttta atttctcttc
     2761 ttctccgact tgcaaatcag cagcagctat ttcttcttgc tggaaatgga gcatatctat
     2821 tctctgaaca taggattttt cattttgctg cacttttcgt accctttttt ccagctcacg
     2881 ataagagata tattcttgct gataactctt tttcttctgt tggaaagcta catctccaaa
     2941 acgatccaat aaagcaagat gtgattcagg ctggagcagt tcctgatgtt cgttttgtcc
     3001 ttggatatct actaaataac tgccgattct tctcagatta gctaatgtga taatatgacc
     3061 attcactcga caaacatttt ttccagttaa ggacatatcc cgtcgaacaa tcagattgtc
     3121 ctcatctgtt tcgatcccta attcaaccat cagttcagag aatccttctt gttttggtag
     3181 ttcaaacaac ccttcaagca cacatttttc tgctccttga cgtatataat cgcttgaacc
     3241 tcttcctcct gctaacagac ctaaagcatc gataataatg gatttacccg caccagtctc
     3301 tccggtcagc gcggtcattc cttcgtgaaa agaaagccgc aattccggaa taatcgcaaa
     3361 gttagtaata ctgatttctt gtagcatcac attcgctcct caacttaaag gtaatgcagg
     3421 aagtctttct tcaataaaac tgcctggtct tcagttcgtg taatcatcaa aacattatcg
     3481 tcatcattga taatcgaaaa taattctttc ttgtaacgtt tatcaatcaa atttgctgct
     3541 gccgaagcat ttcccggcaa tgttttcaaa ataacaaatt tttccatttg gtcaactgcc
     3601 acgaaagcgt cttttaatag tttttccaat ttagtattta catcttcgtt tgtttcagct
     3661 ggtatgctat aacggtagcc gccatctgaa gccggtacct tgatcagttt cagttccttt
     3721 atatctctgg aaatcgtagc ttgagtaaca gatattcctt tgcttttcag gatttcaaca
     3781 aattcttcct gctttcggat atcttgctca ctcaacaatc gagtaatcaa acgatgccga
     3841 tctttttttc tcattacgtc ttcgctcctt agcatattct tcttaatcaa tgatagctta
     3901 tttccttggt ataataaaga agaatccgca tacttctcac ttgattttca cagttacaat
     3961 ttatttatgg tcaaactgtt cgtgtgcatt tgaaaccact tctgccatat taatagctga
     4021 attgattgtt ccgctttcgg gtactttcct caagtgagcc aagaactcga tatttccttc
     4081 tccccccgtg atcggagaat aacttaattc cagcacatca tatccacctt gtgccgcaaa
     4141 tgacaaaatg tcttctaaaa ctttttgatg ggtctgaggg tcacgaacaa ttccgttttt
     4201 ccctacagct tctctgcctg cttcgaattg gggtttgatt aaagcaatca cttctccctc
     4261 ttcttttaaa atagcatgta atggaggcag catcagtttt agtgaaataa aagaaacatc
     4321 gataacagca acttctggaa ttcctaaggt aaagtcttcg ggttttgcat aacgaaaatt
     4381 cactctttcc attacgacga ctctaggatc ttgtcttatt ttccaagcta gctgattgta
     4441 gccaacgtct aaagcataac tcatttttgc gccattttgc aaagctgcat cagtaaatcc
     4501 gccagtggat gcaccaatat ctaacaatgt ttttccttca acagatagat caaatgcttt
     4561 gagtgctttt tctaatttta atccccctcg agaaacataa ggaagttttt tacctttgac
     4621 tcttaattca ctactcactg gaattttttc tcccggttta tcaaaccgct cgtttttttc
     4681 gttgtaaatc aaaccagcca tcactgaacg tttggcttgt tctcttgttt caaataatcc
     4741 ttgtttgacc gctaatacat ctacgcgttc tttttccatt ctttccctca tttctccaca
     4801 tcaaattgct tgatcatctg gcgcatcagc ccgccatcaa aagaaaaaac gtcctcttct
     4861 aatttgtcta tgatcttgtt ggcggataac agctcaatct ccaacgcctc acgtgtcttt
     4921 tctaggccta ataaacgcgg gtaagtattt ttttccatac gttcatcttt accagctgtt
     4981 ttccctaatg ttttcgttgt accaatcaca tcgagtagat catctcttat ctgaaaagct
     5041 aaacctaaat gttcggctaa tcgttgaagt agcaataaga tttcttcagg ttgcttcgct
     5101 aatatgccac cagccagcaa ggcataacga atcaaatgcc ctgtctttct ttcatgaata
     5161 aatgctaatt cttctaaaga aagctttttc gattcgccct cgatatctgc agcttgccca
     5221 gcaaccatac cttgtgatcc cgcacaaaca gctaattgct gaagcagcag aagcttagga
     5281 gaattaccta agtgtgccat actgattagc tggaatgctc ctgtaagcaa accatctccg
     5341 gccaatatag ctaatgcttc gccgaatact ttatgattgg taggttttcc tctacgaaga
     5401 tcatcattgt ccatcgcagg caaatcatcg tggatcaagg aatacgtatg caccatttct
     5461 aaagcagctg ctacttgata gacgggaaca tcaataggct catcaaagga agcaacggcc
     5521 gttaaaagta ataagggacg tattcttttc ccgcctgctt tcactgaata actcatcgca
     5581 tcatagagtc ctctatccgt tgtatgctca tctaaaaaag acaagatttc tttttctaca
     5641 gcaggtagat gaatagctga aaagtcagtt aatctcatct cagctttcct cgtttccgtc
     5701 aaaatcaact tcttcatttg attctgtcat cattttcgtc aaggtctctt ctgctttagc
     5761 taatgtatct tggcaatgtt tgcttaattc cattcctcgt ttaaacgaat ctagagctga
     5821 ttctaatgga acgtctcctt gttctaactg catgacaatt ttttctaatt cttgcagtga
     5881 ctcttcaaaa gttggatttt ccatattttt attctccttt gtctatctct tgtacttgtc
     5941 ctttaacgat tccatcttga taatggatcg tcagcaaatc cccttgatga atctcggtga
     6001 cactttttac tacttgatca tttaacgtcg tatagctgta gcctcttccc atgattttca
     6061 atggactcaa caaatcaaga gattgaacag cttgagcaaa acgctgctgc tgttgtttca
     6121 tatattgttg gattttctct tctaagcgtt tttctagata agcagtttct tggcgagctg
     6181 ctttaactcg ataaagcgga gccacttgtt ccaattggtg gcttaatgcg atcgtttgtt
     6241 tttctttcgc atgataaatt tgctgtgtag cttgatataa tcgctgtttc aactggtcta
     6301 atttgatggt ctgaccttca tacaatcttt caggctgccg aaatacatag gaacgtcgag
     6361 cacgctcgaa ccgttcttgt ttgcgctgga tctggcgtac aaacgcctgc tgcaatctag
     6421 cttgccgttc ttgaatgcgc atcaactctt ctgacaaaac aggaacagct aattctgcag
     6481 ctgctgttgg agtggcagcc cgaacatctg ctactaaatc agcgatcgtg gtatctgttt
     6541 catgtcctac agatgaaatc accggcgtat tagcttgata aatcgcccga gcaacccgct
     6601 cttcattaaa cggccataaa tcctcaatcg aaccaccccc acgaccgatg atcattgtat
     6661 cgaaattgcc catctcttct actctctgga tattcctgac aacattgtcc gctgcttggt
     6721 ttccttgtac aactgttggg aacaaaacca attggacaat tggatatctt cttttcaccg
     6781 tcgtgatgat atcgcggata accgctccgc ttggactggt aaggaccgcg attcgctttg
     6841 ggaaacgtgg cagcattttt ttaggccctt gaaaaaggcc ttccttcatc agcttttctt
     6901 ttcgttcttc caattcttga tataaagcac ctattccatc aggctccatg tgctctacat
     6961 agatttgata agaacctccc gcttcatata atgaaatacg cccaacgaca aggactttca
     7021 ttccttcttt tggctggaat cgtaattttt ggaatgctcc tttgaacata atagcagata
     7081 tttttgcatg gtcatctttt aagctgaaat attgatgggc attggcacgt aaacggaaat
     7141 tcgatatttc gcctgttaaa tatacgcgtt ctaaataagg atcggcatca aattttcttt
     7201 tcaaatactt cgttaatgta gtgacagtta aatattcttg tgccatctat tcacctgctc
     7261 caaaagcttc ggcagatttt acggtttgat acatcagcat cgtgatagtc attggcccta
     7321 caccctttgg cacaggagtg atccagcctg caataggctc cacttcatca aatttcacat
     7381 caccgatcag cttaccgtta gcatcacgat tcatccctac atctatcact acagcacctg
     7441 gttttacgaa ttcttttgtg acaaaatgtc cacgtcctat tgctacgact aaaatatctg
     7501 cttcttgtgc cagttcagcc agattttcgg ttttagaatg agcaacagta acagttgcat
     7561 ctgccatcaa gagtagctga gccatcggtt taccgacgat attgcttcgt ccgataacta
     7621 ctgctcgttt acccgccgga tcgatagaat aagcttcaaa cattttcatg atgccgtatg
     7681 gagtacaagg aatcttgtcc ggtttacccg caaggagtct gcctaaattc atcggatgaa
     7741 aaccgtctac gtctttttca gggcggatag ctaacaggat cttttcttca tctatatgct
     7801 ttggcaatgg taattggacc aatataccat gaaaactagg atcttgattg tatttttcaa
     7861 tttctgcaag tagttcgtct tcagaaatcg tctcaggata acgctctacc aatgaatgga
     7921 ttccgatttc ttttgctaca cgttctttat ttcttacata gatttggctg gctggatttt
     7981 cccctaccaa taaaacgact aatccgggat ggataccatt atctttcaat ttttggattt
     8041 tttcagcaat ctctgcctgc attttttcag ctaattcttt tccgttaatc aattctgcca
     8101 tctaaccact cccagatatt ttttgtgttt tcattctacc atagaatttt cccgatttat
     8161 aacaggttta tgatgattca tagaaaaaag ctaggactta cgccccagct tctaattctt
     8221 tcaatacatt ggataaaaca ccgttgacga atttacgtga acggtcatca ctaaatgttt
     8281 ttgccaactc gattgcttca tttaaagcaa cagccgctgg tacatctgtc acataagtca
     8341 tttcaaaaat cgcgatacgc aagatcacca aatccatttt tgagatccga ttgatgtgcc
     8401 aattgttttt taaatgtttt tcaatcactt tatccaattc atcttttttt gcacagacac
     8461 cgccaactag cgtatcaaga taaacaggaa caaactctga ttcatcttcg ttgatcatgt
     8521 cgcggtggtc taattcgatt gcgttgaaaa tcgcatcttc ttttgttagg tctgcattga
     8581 agtctaatgg aaataaagcc tgcaatgcca tttcccgaat ctcgtgtctt gataattctt
     8641 tattcattct cttcctcttc gtcttcaaaa agttcttcga ttgttggttg ttctaatttt
     8701 tcaggaacaa cagctactac atggatattg acttcagata aatcgatatc cgtcatgaaa
     8761 aggacttgct ggctgacccg ttcctgcatt tccattgcta ctttaggaac tgaaacacca
     8821 tattctagat aacagtaaag atctactttc aagccatctt cctcgtttgc taagtaaaca
     8881 ccttttccgt gagctgcacg tcctaataat tcagtgacat tgctggcaaa agttccgcgc
     8941 attccgtata cgccttctac tttagaagct gcaatcccga taattacttc gatgacttca
     9001 ggagcgatca cgatctctcc taaatcttgg tttgcgttta aaatcaaatt tttttcatct
     9061 gccatttatt tattcctccc tttgcctaag cacgagatac atccgaattc tctatgatgc
     9121 ggcatagtga tcacgtggta agaaaactgc cgccgatcag ctgcaaactg ctatgaattg
     9181 gagaaaacac ccaactttat tatctcttac agcttaaaag ttcttctata gtgtatcatt
     9241 aaatcgagtg attttctata acaaagtctg tgaacactga tttttacgct tacttcttct
     9301 attgatgaaa ctataagatg atcaattctt ttggtgaacg agtcaacaca cgatttccat
     9361 cttctgtgat caacaagtca tcctcaatac gtactccacc gattcctggt aagtagatcc
     9421 ctggttcatc agtgatcaca tttcctggta caaattgctt ttctgcacga gaagaaacgt
     9481 ttggtccttc gtggatctct aaaccgatcc catgtcctgt agaatgtcca aatgcttccc
     9541 cgtaaccatg ttttgaaata taatctctag caactgcatc taattggaca ccagtcattc
     9601 ctggttttgc agcatcaata actgctaatt gagcatctaa gactatttga taaatttctt
     9661 ttaatttttc cccaggatca ccaatagcga atgtacgtgt catatcggaa acgtaccctt
     9721 cgtaatagca accaaagtcc aatgtgatca agtcaccttg ttcgatgatt tttttgcttg
     9781 ctacaccatg cggcatagcc gaacgcaatc cgctagcaac gatcgtatca aaagaaacac
     9841 ctgaagcgcc aagagaacgc atataaaagt ctagttgatt tgcgacttca atctcagtca
     9901 tgcccggttg gatcattttc aggatgtgat catatgccat atccgcaata tgacatgctt
     9961 tttcgataat agcgatttct tcttcatctt tcacttctct gagttcttcg atcatctgtg
    10021 aaacaggtac taattccgca tcgatgattt cttctaaaac cgaatattct aaaaatgaga
    10081 ctgttgtttc ctcaaaagct aatgagtgca gttcttcttt gcgtactaag tttgctacct
    10141 catcaaaaat aggtccgaca tttttaataa tttcaaaccc ttgagcctgc gctgccgcct
    10201 gctcggtata acgaaaatca gtaataaaaa atgctttttc caaagtaata acagctaatc
    10261 ctgtcgtacc tgtaaagttc gttaagtaac gaagattgta cggactggta accaaaaatg
    10321 aatccaaatt ttcttcttgc atttttttgc gcaatttttc tacacgtagc atcataatga
    10381 aactccctct ttctttgcac gcttttttcc attatagcaa acttttcttc gtacacgaca
    10441 gttaaataaa aatttccgga aaacttcttt ttattttaaa taaataaagc caaaaaggag
    10501 tcacagtctc gctgcaactc cttttagttt actattttgt aaaaacttat gatcgttttc
    10561 caaagaaaaa tgagacaact gctaccaaaa tcactgcgcc taaaatagat ggaataagtg
    10621 ccatacctgc agcttttggt ccccattcgc cgaaaatcgc ttgtccaata cttgaaccga
    10681 ctagtccagc aatgatattc gttacacagc ccatcgaatt tcctttactg gtgatcgcac
    10741 ctgcaattgc tccgatcacg gctccaacaa tcaatgtcca taaccaactc ataatataac
    10801 ctccttcttt gctaatcaat tttagcatat gggatttaaa ggggacaaat aattggtttt
    10861 gctatttcat attttcttct gcaaagtaaa aaaataaaga aaacctcctc ttagaagttt
    10921 tctttatttt ttacttgcgt ttaaacatac tacgaatacc acgtttcact acatcaaaga
    10981 aactcaatga ttgtaccatt ctatcttcat ctacatattc tctgatcgca cgaagcactt
    11041 ttttaggttc tttggaagaa aatgtgtatg taccattttt tttcgtttga atggcatatc
    11101 gaggaatcca ttttcccttt agcatgactg atgctagtac ataatccact tcttcccatg
    11161 gaatttggat atatttgcgt ctatctcgcg catggtaaaa ctcaaaacca ttatcaccta
    11221 tcatgatttg cccgtaatct gtcagacccg taaacgaagt agcatcaatt accaagtcta
    11281 cttttgtatt gattgattga accattttct ccctccagat atttgtttat gaacagtata
    11341 ctatataaaa aaagctcggg acaacgcccg agctatattt ttatggatta taaaagtccg
    11401 atgacgtgtc caacaacacc gactacgaat aaaccaagga tgataacgat tggagaaacc
    11461 tttttcttaa gcaaccacat acataacaat gttaatccta atgcagctaa accaggaatc
    11521 aattggtcta agttgtcttg taatgttgtt accttttcag gagaaagggc taagccatta
    11581 ttaacttgtt caaatgcact cttgattcct tcaccacctg aaggcaattt atcccattcg
    11641 atgtaagcac ctttatctaa ttttacagtt gatacaactg gtaagaattt gattgatacc
    11701 catctttgta caagggcagc taatacgaac atccctaaaa ttgaagcacc ttttgttacg
    11761 tcttgtaaca atccgccaga aaggtcatca gtaattttag aaccagcttt gtagccaaat
    11821 tcttgtgtat accacatgaa tgcccaacga ataatgttcc aacctaagaa gaagatgatt
    11881 ggtccaagga tatttcctcc catagctaat gaagcaccta gtgcacctag cattggacgt
    11941 actgtaaacc agaacactgg gtcaccaaca ccagctaaag gacccatcat acctacttta
    12001 accccttgaa tcgctacatc atcaacaggc gcgccattcg cacgttcttc ttccagagcc
    12061 aatgtaaccc ctaaaattgg agaagcaatg tatgggtgtg tattaaagaa ttccaagtga
    12121 cgttttaatg ctgcagaacg ttcttctttt gttttgtata attttttgat tgctggaatc
    12181 atagaaaatg cccagccacc gttttgcata cgttcgtagt tccatgaacc ttgaatgaag
    12241 gtagagcgcc acgcaacagc taaacgatct ttttttgata atttgatttt ttcttctgcc
    12301 attttatttt cctccttcaa gattaatagt cattcaaaat gtcgcctaaa gggtcaccag
    12361 agttgcttcc gcctccatta tttgatgaac cacccatttt agaaaggttc aagtaaataa
    12421 gagctaatgc aacacctaaa gcacctagag caatcaatgt taattgagaa attgcagcta
    12481 caacgaaacc aataacgaag aatggccata cttcttttgt agccatcatg ttgattacca
    12541 aagcgtaacc tacagctacg accattcccc caccgatagc cataccatct gttaaccatg
    12601 ctggcattga ttcaaggaat gattgcacag tttgtgctgg gataaatagc aacgctgctg
    12661 ctgggaccgc gatacgaacc ccttgtaaac atacagcaat gatgtgccac atttcaacct
    12721 tacggatgtt tccttcttca gcagccgcat ccatcaaatg aacgattgga acagcgattg
    12781 tacgtacgat cattgttaag aacaaccctg caacagccaa aggaacggca atcgcgatcg
    12841 cagaaggaac acctttaaca ccttgtccac ctaaaactaa aataattgct gatgctactg
    12901 atgctaatgc agcatctggt gctacagcgg caccgatatt tgcccaacct aaagcgatca
    12961 tctgaagcgt accgccaagt acgataccag cttcgaggtt accggtaact aaaccgatca
    13021 atgtacatgc cactagtggt tgatgaaatt gaaattcatc aagaatacct tccataccag
    13081 ctagaaaggc aactaatacg actaaaatta ttgaaataat agacatgata agcctcctat
    13141 aaattttaat attatttatt acgcttttgc taattcagct tttgcttttt tcaaaatatc
    13201 atccatgtta tcgcgtgaat cattaggtac tttacgtaca tcgaatttca cacctttttg
    13261 ttcaagcttc tcaaacgctt caacgtcttc ttgtcccata gacaatactt tacttacaac
    13321 gactttaccg accgaatgcg ccattgaccc aacatttaat tctttgatgt ctacgccacc
    13381 ttcaacagct gtcaatacgt cttcaggatt ttcaaataaa agcaatgctt tggtatttcc
    13441 aaaacgaggg tcttttgcta cttcgatcat tttgctaata ggaataacat ttgcttttac
    13501 acctggagga gcagcttgtt cgattagtct cttacgcaga tcatcctttg atactgcatc
    13561 tgaaacaacg ataatacggt ttggttgcac tgcttttgtc caagctgtcg caacttgtcc
    13621 atgtaacaga cgtgagtcaa cacgtgctaa tacatatttg atttttccgt ctccgaccac
    13681 agttccttct ggaagtgctc ctttaggttg tgcgtcttca actgctgctt tcggtgcttc
    13741 tgccggttct aattcttccg gttttacttt tacaccgtct tttgctgttt caatgatgtg
    13801 tgctgcaatt tcatgagcag attccattga aaagcgtgat gcgtaggctt caatcaacat
    13861 tggtaaattc aaaccgctga cgattgccca tttatctttg tgctcttcga ataatgtgtt
    13921 tgattgattg aacggtgttc cgccccataa atcaactaag aataacactt cgtcttgatt
    13981 atcaaaagaa gcaaccgctt cttccaattt ggctctcaag tcatctggac cttcacttgg
    14041 tttcaaaatc acggctttga cgttttcttg ttcgccaaag atcattgaac cggattgtaa
    14101 gattccttca gcaaattgtc catgacttgc taggataatc cctaccatat ttatacctcc
    14161 tatttacttt tgttgtaaca aattcaaaaa tattaataat tgctaaccat tacaacagaa
    14221 ttatttgacc ttttccaaca accattcttg ctacaccttt ttgattttcg ctagtagttc
    14281 catcaaaagg attttcctgt cagcaggtac ttttcgaatc tctaactcca cttcttgact
    14341 gttcaagtaa cgaaaggaat caatatcttg ttgatttact gaaacaaaat tggtaatcat
    14401 tgatttgcct tcagtaaaac tcataccgcc gatattcaac gacttcaact gtacaccgca
    14461 gcggacaacc gcttcaacat cttggggatt ggtgaataaa agcatcactt ttgctcgtga
    14521 taataatcca ctatgataga tttgaatcat tttctgcacg gttattacat ttgctgtgat
    14581 ccccggcggt gcagcttctt ttagtagaaa ctttctcaat ttatcttgag cgactacatc
    14641 gctgatcaca atgatacgtt caacacctgt ttgcttcgac caaactgttg cgacttgccc
    14701 gtggattaaa cgatcatcta tacgtgccaa tcgaatatcc atctgcaaaa ctccttcttc
    14761 ctcactactt cacagaaacc cctatcattt ctgtgtgaac aaccacctcc tagcacacac
    14821 tactataagc aaaaagcgtg ccaacttttt tacgatacac taattgaatt taaaggattc
    14881 ttaaccaata cacaaaaacg atacactata aaagtgtatc gtttttgtgt attcatggca
    14941 cagtgtattc ttttttatct aactgattat ggactagacg tagcagataa tacatttctt
    15001 ctatcggcac ttttactttt atctgatctt ccaacagaac aactgaggct gccaattggc
    15061 tataaagagg atcattcgtt gtatttgtca attcgttttc ttccacagtc aatggttcat
    15121 tccgaataat acgttcgatc attcctgctg tgtgcatcac taggttgatt ttgaatccat
    15181 attcttcatc gccgattccc gtcgtctgag agatttgtgt agaaaattgc cacattggat
    15241 cgattaactt actaccatta ataaagatga agttatcaga gataaactca acacacgtat
    15301 ttttagctga ctcttcatct agggagatga attcagtttc ctccaattct gattccatca
    15361 gcaactgatc caaaatgact tcgatatttt gatcaataaa acgttctaaa ggaataaatg
    15421 gggcttggat attcaaatca gtaatcccag tagtagctat gatctgatat tctttttgga
    15481 taactggcag ttcttctttc acttccacaa tcgacaaagc tagaacttct aattctgttt
    15541 cttgacgctt gtttaccgct cgttcgatca attctttgat tctttgtgct gtaccttcgc
    15601 cagatgcgca gatagccaaa acagcttttt tcttatgaat aggttttgct tcctgaacag
    15661 attctacaga agatatttct gcgtaacctc gaaaattttt caatgattca tgcaacatat
    15721 ctagatctgt ccctaatact gagacctttc gtacagtttc caaaactaga gaagttgtta
    15781 ccatatcaac agtacgtact ggaattccag tgtctctttg aatttgcgaa ttaaacgacg
    15841 caagggaccc catatctact aagagtatga ccccgcttcc ttcatctact tcttgtacat
    15901 tgcgttcaat tcggtccaat gctgttttcg gatccatatc caaaggcatg tctactgctc
    15961 gtacgttctc tgcatctaac aactgttgga caacttggac catactgctt gctgtgctgt
    16021 ttccgtgagc tgcaacgact acaccgattc tgccgcttgc ttgatttgcc cgtaacgaga
    16081 cgagtagaac ggtcaagtaa tagtcttcac tttctggaat ctccacttga aaatacgttt
    16141 cgatataact acgcacttct ttcgcaacag catattcatc tggaaaatca aaagccatct
    16201 cacggatgtt atcattcgta tgccgttctt ctcctaagtt gatttttttc aaaaaagaac
    16261 tgatatgcaa gctcatagca taaataaagt tttgctgaaa ctcatatggt aaagatcttt
    16321 tgaccattac ataaatctga ttggtcactt ctatgatttt cggatctaca aattctgcta
    16381 atttgctgtc tgcattaaaa gaaaaaccat ggtctttata gaaagacttc agatggatgt
    16441 tgatatctgt agaaatgaaa tgattgattg cttcttgatc caaaccatcc gacttcagta
    16501 aagctgcttt atctccgatg atatcatata gattataagg taactcatag gagtccgatt
    16561 ggattttcat gatcgtatca tttggtgcta cagttatctt tggctccaat attttagaaa
    16621 gctctgccat tgcactgcgg ttgcttgcta attgaatcaa tccgctacga atcccttctg
    16681 tcaaatcatc gattgtgatc gagatttcgg gtgattgcat atgatttaga aatcctcgtg
    16741 cacatactaa ctggatattt gacttcaatt gtcctatatt tccataggtc acactgccga
    16801 tcaatgcttt tgctacatct tctgtcaaag aaatctttcg ctgcgtgcgg tttgcttcgt
    16861 gggcaatcat gatttttact aaatcgactt tctcatttgc aggacgtttt tcaaaagccg
    16921 gaagtttgat attgattggg atacgccgaa caaaagtttc taatagagaa gaacctgggt
    16981 cttctgtggt cgcacctacg atccgtacat ttgcttcatg ggatttcgtt gtttccccaa
    17041 gacgactgta agttccatga tccataaagt aaaagatcat ttcttgacct tcaggcggca
    17101 accggtgaat ctcatccaaa aacagcatac cgccatccgc ttgatcgata atccccattt
    17161 tttcttcatc tgcgcctgta aaagcacctt ttgcgtaccc aaataaatga ctcatcaaaa
    17221 gctcagggtt attggcataa tcagcacaat tgaaaacaat cagttccgtt tcctcatcta
    17281 ttacatgatg cgcttttgca aaatgataca tcgcgtgtgc aaaataagtc tttcctgatc
    17341 cggtaggtcc tgtaatcaaa caatttaatc ctcttggcgg atacaaaatc gcagcttttg
    17401 cctgttccac tgaatttttc atgctgccgt tagctccaat gatttttgca aagatatcct
    17461 tagtatctat ttggagtgta tttttttctt cagagaccgg cgtttctttg taactgacta
    17521 catgttttgt aactggcttt acgtgcgtaa cgagtgtttg atatatgtaa cgtactggtc
    17581 gtccgtctgt ctttatcagt tttccttcgc gcactagctg attcagatct ttactgctat
    17641 ttgttcgctg aatccctaac acttcagcaa tttcctgtgt tgttactcct tgatcatatt
    17701 ctgcctttgt taaatgagcg gttttttctt tgacatacat ataaattcgg tcaatgcgtt
    17761 ttgccaatgg tatcgctcct tttctataga ctattgtact taggcttcaa aacataatac
    17821 tatgtattta tttctgtttc gtcaactatt tacctgattt tgttttgttg ataacacgcg
    17881 gatagataga aatgttcaat ttaaaagaac gaaaaatccc gatacgattt ttataatgga
    17941 aacaagaaaa agaacactgt ttaccatata attatttcac tgatagcaaa caatatagct
    18001 tccatcaatt gaaaacgata gttatcaaaa catcctgatt ccaaattggc aaaaaaatat
    18061 taagtgtaaa aagacaaagt aaacttcgta gcctccacta caacttttat ttttataaac
    18121 aaaaaaggct gtgacaaaag tcttgtcaca acctctttaa tcataagatt caattaagct
    18181 tcttgagcta ctggataaac agacacttgt tttttgttac ggcctttacg ttcgaaacgt
    18241 actacgccat caacttttgc aaataaagtg tcgtctccac cgatacccac gtttacacct
    18301 ggatagattt ttgtaccgcg ttgacggtaa agaattgatc cacctgtaac tgtttgtcca
    18361 tcagcacgtt tagcacctag acgttttgat tctgagtcac ggccgttaga tgtagaaccg
    18421 ccaccttttt tgtgggcaaa taattgtaga ttcatagata acaacatggt ctgcacctcc
    18481 tatttatcat taattgtttt aatttggata tattctgagt tttcagcttc tatcgattgt
    18541 aatcctaata aaagattttc taatagaatc tcagcaatat tgttttgttc ttgtgtcatt
    18601 cctgaagtga cttcggtata aagataaccg ccttcttctt cattcatttc aacaatcggc
    18661 tcaaaaccgg ctaatgaggc aatcccgttg acagtgctga tcgctaacgc agaaacaccc
    18721 gcacaaacga tatcacttcc ataaggacca gcatcagcat gcccggtcaa agtgaatgaa
    18781 acgatctgac ctgcgtcatt tcgcttaaat gttcctttga tcatatatag gccttctttc
    18841 tcaaacgcta ttaagcgttg atcgcgtcga taaccacttt tgtatatggt tgacgatgac
    18901 cttgtttacg gtgtgaatgt tttttaggtt tgtatttgaa agtaacgact ttcttttgtt
    18961 tgccgtgttt ttcaacagta ccttcaacag ttgcacctgc aacagttgga gcgcctactt
    19021 ttgtagattc tccgcctact aagataactt cgtcaaaaac aactttttct cctgcttcaa
    19081 cgtcaagttt ttcaacatag attgcttgtc ccacttcaac ttttacttgt ttaccaccag
    19141 ttttaataat tgcgtacatg ctataaagca cctccttctg atatacttag actcgccatc
    19201 cgaagtgaca gcatatgctg tgcttattac ttgttctgag cggttgtagc tgtggagtcc
    19261 acaattacaa cagtactagc ttaccagaac agcaaccgtt agtcaactaa atttaaaatc
    19321 aaaaagtaaa acaaaaattc aagcaatact tctgcttttc tatcctcaca gttctaccaa
    19381 ataaaatagt cgcattgtgt ttcttttatt cttataataa aagaaaaagg aggagcataa
    19441 atggaatacc caaaagaact gatacaagaa gcttctttaa gaatgaacgg ccgtcctgta
    19501 gaaggattta tcgctgggca aggacctctt catcctaaac tgatgctcgt gggagaagcc
    19561 ccagggaaaa cagagattga tacgcatgtt ccttttagcg gtcaagctgg aaaagaattg
    19621 atgcaggcac tttcttcaac tggcttaaca cgagaagagg tgtatatcac cagtgctgta
    19681 aggagccgcc cgtatcgcgt gactcatcgg atcaataaac ggacacaaca gtcagaaact
    19741 gtttatccaa atcggacacc tactcgttcc gaagtatttg cgcatgcacc aatattggat
    19801 tacgaactga tgaaggtcca gccaaaatta attgcgacac ttggaaacat tggtcttcaa
    19861 cgtttacttg gcaaagaata tacggttacc aaaaatcatg gtcaactcta tacagggcct
    19921 gtcttacaat taacagaaca aaaggatgcc tacgaatggt cagaaaaaaa ctatcgaatt
    19981 cttccacttt ttcatccagc agctattttc tacaatcgct cccttgtacc tgttatccat
    20041 caagactggc aagtactagc ttcactttta cagcaatatt aaaaagaaca gacaagacca
    20101 taaaatcttc agtttacagt cttgtctgtt ctttttcatt gttttaatgg ctaattgcag
    20161 tatatttcgc atgtaaagct gtcaacgcat gagcaactgt ttgatgttga tgcgtgaatg
    20221 tatgttccca caagtgagaa agttgttttt ctacttctgg ctgcaaggaa gtatagataa
    20281 tcgtttgtcc tctttgctct gctttttctt gaaattcatc aaaaactgca cgtgccgtca
    20341 aatcaatact tggtacgcct cgcatggaga aaatgacacc atcgtgatga tttagttctt
    20401 ccaatttatt tttgagtttt tctgaagaca taaaaaacag tggaccacta atatacacta
    20461 ctgtccagtt attcagcttt tccgtttctg gcagccccat tctttgccaa tcgatcgttt
    20521 ctaatgaaat cgtgatagca gcgcttttga caacaaaaaa tacacagccg ccgatcaccc
    20581 cgatcacgat agcaatactt aaatcaaaga taactgtaca aatcatcgta aggataaaca
    20641 atagaactgc tgaccaatat ttattagtaa atagctcctt gatcgtttcc cattcattca
    20701 tacgccaagc cgtcacaatc aaaacacctg caagtgctgg cattggaatc tgtgacatca
    20761 ctggcgcaaa aacaagcatg gagagcagta agacaactgc atgtatcata ccagctaatc
    20821 tagtttgtgc accagatttg atggccacgc ttgtacgggc gatggccgca gtagcaggaa
    20881 tcccgccaaa gaaaggaagc aacagatttc caattccttg cgccattaat tcttgattgc
    20941 tatctaaggg cttatttgcc attcggccag ccgaagcacc acatagcaag ctttcaatca
    21001 ttcctaataa agcaatgcta atagctggaa ctgccacttg ttgcaaggca gaaaagctaa
    21061 aatctcccat attcaaacga ttactactga tcaatgtttg tggaatctta cccacagttg
    21121 ctactggtaa atggaacaat agcataactg ctgttgccaa aatgatagcc agcagcgaac
    21181 taggcattcg tttcgcccat ttttttggat agataaacat ccctaaaatg actagccctc
    21241 ccatgatcaa agaggctgtg tttatcggaa atcctatcgt ttgataacta cttagttttt
    21301 caataacgtt tgtaccttcc gagtgaacac caaataaatt atcgatctga cctaatgcaa
    21361 taatgatagc aatacctgaa gtaaaccccg taattacggg tgctgggata aaagaagtca
    21421 gtgttcctaa tcggaaaagt cctgctgcaa gtaaaaatag acccgctaaa aaagtagcaa
    21481 gcaatacacc ttgcattccc tttgttgccg caatagacat caagatagct gccatcgcac
    21541 ctgtcggacc tgatatttga taaaaaccgc ctgataaacc gccaataacg ataccggcaa
    21601 tgatagccgt aatcaaacct gcggccgcat cagctccgct tgatacccca aaagccaagg
    21661 ctaaaggcaa tgctacagca gctactgtga tcccagccaa aagatctttc tgaaatcttg
    21721 aaagatcata acctgaaaat tcttttttta gtgaggaaac ataatttttg aacacctatc
    21781 taactccttt ttcatgaaat t
//
LOCUS       DAHUWM010000024        21696 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHUWM010000024 DAHUWM010000000
VERSION     DAHUWM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.024"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            16..570
                     /locus_tag="NK788_000645"
     CDS             16..570
                     /locus_tag="NK788_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6768995.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(743..1216)
                     /locus_tag="NK788_000646"
     CDS             complement(743..1216)
                     /locus_tag="NK788_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6768996.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1228..1764
                     /locus_tag="NK788_000647"
     CDS             1228..1764
                     /locus_tag="NK788_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6768997.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1804..2175
                     /locus_tag="NK788_000648"
     CDS             1804..2175
                     /locus_tag="NK788_000648"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6768998.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2459..3730
                     /locus_tag="NK788_000649"
     CDS             2459..3730
                     /locus_tag="NK788_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCH6768999.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3975..5000
                     /locus_tag="NK788_000650"
     CDS             3975..5000
                     /locus_tag="NK788_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6769000.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5294..6403
                     /locus_tag="NK788_000651"
     CDS             5294..6403
                     /locus_tag="NK788_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCH6769001.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6486..7382
                     /locus_tag="NK788_000652"
                     /pseudo
     CDS             6486..7382
                     /locus_tag="NK788_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7398..8222
                     /locus_tag="NK788_000653"
     CDS             7398..8222
                     /locus_tag="NK788_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769002.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8254..9564
                     /locus_tag="NK788_000654"
     CDS             8254..9564
                     /locus_tag="NK788_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCH6769003.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9712..10971
                     /locus_tag="NK788_000655"
     CDS             9712..10971
                     /locus_tag="NK788_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6769004.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10981..11853
                     /locus_tag="NK788_000656"
     CDS             10981..11853
                     /locus_tag="NK788_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769005.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11865..12695
                     /locus_tag="NK788_000657"
     CDS             11865..12695
                     /locus_tag="NK788_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769006.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12735..14918
                     /locus_tag="NK788_000658"
     CDS             12735..14918
                     /locus_tag="NK788_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCH6769007.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14979..16199
                     /locus_tag="NK788_000659"
     CDS             14979..16199
                     /locus_tag="NK788_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCH6769008.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16196..17656
                     /gene="gtfA"
                     /locus_tag="NK788_000660"
     CDS             16196..17656
                     /gene="gtfA"
                     /locus_tag="NK788_000660"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCH6769009.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17733..18839
                     /locus_tag="NK788_000661"
                     /pseudo
     CDS             17733..18839
                     /locus_tag="NK788_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19161..19370
                     /locus_tag="NK788_000662"
     CDS             19161..19370
                     /locus_tag="NK788_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCH6769010.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19363..19512
                     /locus_tag="NK788_000663"
     CDS             19363..19512
                     /locus_tag="NK788_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCH6769011.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19666..20331
                     /locus_tag="NK788_000664"
     CDS             19666..20331
                     /locus_tag="NK788_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6769012.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20615..21685)
                     /gene="xerS"
                     /locus_tag="NK788_000665"
     CDS             complement(20615..21685)
                     /gene="xerS"
                     /locus_tag="NK788_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCH6769013.1"
                     /translation="MYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKNYKLFY
                     EWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLS
                     TSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARANAIEKK
                     LFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSEVININ
                     LDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIY
                     QGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSS
                     TNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7367 a         3275 c         4187 g         6867 t
ORIGIN      
        1 agaagggaaa caatagtgag ctataatgta caaccgttaa ggacaaatga agagatacaa
       61 gattttcagt tttggctaag aagaacaagt aatccggaaa gagatagttt tttatttttg
      121 tttggaatca ataatgggtt acgtatgtca gatattatag gattaaaagt tggagatata
      181 cgaggcaaaa gtaagcctat tattgttgaa cataaaacag gtaagcgaaa accgattttt
      241 atagataatt tgagagaaga aattttatta tatactgaag gtaaagaaga aaatgattgg
      301 ttatttccga gtcggcaaca aggtcgacat ataacaaggg atcgtgtcta tcagatatat
      361 gcagatatag cagaaaaatt aggacgagat gatataggaa cacatacatt aagaaaaact
      421 tttgggtatc actactacaa gaaaactaga gacattgcta ccttaatgtt tatttttaat
      481 catagctcgc aagcaattac aaaaaggtac attggtataa ctgaagatga aataggagca
      541 tcacttcgtg gatttaaact aggtgtataa tttaaaaatt tttataaaca gtaaagacaa
      601 agattaaaag ctatttttat ctagaacgat tttatttcat aaagttcgcg gaataaaagc
      661 tggggttact ggtacttggt ataggttcgt gttatataaa ctaagtaatt aaataacatt
      721 tttaggttgg aaaaggatga aattaaaaaa agagaatcaa tacggtctca aaaacgcaaa
      781 cgctgcggaa taagaaaaat actgataact caacgtttag cagactatct aatagggata
      841 gtctgctttt ttcgtttttg accatacttt tgaccttctg caaacttcct tgcttttcct
      901 ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc ctctgtctgc tttttcgcga
      961 tatgggtgta tatatcatcc atagtggttt taatatctgt atgtccaagt cgaacataca
     1021 cttctttgac agacgcacca gattcaaaga gcagactaca atgggtatga cgaaaaccat
     1081 gaagtgtgat atgttttaag ttattctctt taatgatgat tttcaaattg tgatttacat
     1141 agtctaaata aaggggctta ttttcttctg atgaaaacaa aagttgttca ccatctttta
     1201 taggtttgat tctcattttt agatattatg cccgcacgta ttcatcacat ttctattgtg
     1261 aatcgcttta ttcgaccgac atttgacttt tatcataata tactcggttt aaaattacta
     1321 atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc tgtttttttc agataatcac
     1381 caccgtgtgg gaactgaact gacttttttt gaagtacaag aagggaacaa ccagtttttt
     1441 ggaaccaata cgattgaacg aacgatacta aaagttcctt ccgaagcatc attgcatttt
     1501 tgggaaatat attttgaaac acaaggcgtt tgccattatg gaatcgaatc attttctgga
     1561 cgacctatct tacgttttga agggccagat gcaacacgat tagctttagt tcccttacgc
     1621 gaatttgaag atattgatga ttatttcccg tatgtcacag accagatccc tacagagcat
     1681 gccattttag gaattgatgc cattcaacta cgcgtacaat attccgatgc gactgaacga
     1741 gagttattgt cggtaggctg gtagcacaaa gaatacgtca gtttcttttc gggtcatcaa
     1801 cgagtgacaa tcttagaaaa tcaagacact cagttctatc aagaagttca gattattcaa
     1861 gatcgtgaga atccagttgc cattgaaggg attggtagcg tccatcatgt tgcgtttggc
     1921 gtagaaaata aatctgattt acaacgcatc gacaaacaat tacaagagcg caattttata
     1981 aattcaggaa taaaagatcg tgaatttttt gtatcacttt attatcgtga cccaaatcaa
     2041 ttattgattg aaattgctac aggggaagga aacctagatg caaaagcgta tgaaaatcaa
     2101 agtccacgct ttgaagaaat tcctttattt ttaccgcagt acttaaattt cattcgtgaa
     2161 caagtggagc aataattagc taatcaaatc catgacgaag agtaagaggt tgactcataa
     2221 gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag aatgttgatt tgatgcggtt
     2281 cctattttta taaactatca ttttcgacta ataaaactac ttatgagtca gcctctcttt
     2341 ttttcttgat ttttatacct ctttctaggt aaactaaaca tctgccaaaa ataccgtgtc
     2401 tcattgaaac attctcaact atttcaaaat aaaggggagt accaaatgaa cgagtatatt
     2461 gaaattatcc acgcaagaga aacaacttaa aaatgtcagt ctcaaaattc ccaaaaataa
     2521 aattagtatt tttaccagcg tatctggttc aggaaaatca tcgattgttt ttgatactat
     2581 tacccaagaa gctggtcgtc aactgaatga cacctatgat agttttaccc gtttattttt
     2641 acctaaatac agtcgtccag aggttgatga gattgataat ctttctacgg cgattgtggt
     2701 caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta gggacgatta ctaacatcaa
     2761 tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct agtttaggat atgcaaatgc
     2821 ttattcattt aatgatccta gtggaatgtg ttctgtctgt cagggaatcg gtaagacgat
     2881 tgcattttta ataggattgc tttatttttc ggctattgat aatgctgatt ttgtattttc
     2941 tgatccattt accgcagaag ttttgtctgg aattgaggaa caggcaaaga atcatggtta
     3001 ttttgtattg gtgcataatg ttaattcagc agaagatgtt aaaactattc agagaaattg
     3061 gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa gaatttttgg aattaaataa
     3121 cgtttttgat ggtcctgttg tttatattga tacgcatctt tcaaaagaga cattaagaga
     3181 acttgaacaa caaatgaatc gtttatttgt aaatacaaat gattaccaag ctagtacatt
     3241 ggcagtggaa catttgattc attatggtca tgagaaaata gcgtttcttt cttttgacta
     3301 cattgtaggt gaaccaagtg ttattcaaga acgacatcaa gcttatatcg atacattaaa
     3361 gaaatatcat ttaaccttta aatcgcattg gatatataac aactctgaat ttgagcgaat
     3421 ttatcaagaa ttagctgaat ttactgcaat tgttgttact ggagattatt tagcggctaa
     3481 attagtaaaa tttttaaaaa ataagaataa acgagtgatg gaacaattat cgattattgg
     3541 atttgatgat attacttttg cagaattaat ggatcctgcg ttgacaacaa tacgactaaa
     3601 tccaggtaac aagggggcga ttgcttttca ccaactgatt ggactggcca aacaagaaga
     3661 aataaaaaat caagtactac tattagaggg agaattgatt tctcgagact ctgttcacaa
     3721 aatcaaataa aaaagctctg tgaaataagt atagttaaca ataaaatatc cgaacgatag
     3781 agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt cactagaatt agcattattt
     3841 actctaaatt gttcggatat tttttcttgt ttttaattat gattacagtg cctattttta
     3901 acgaaatttg cttattttca aaatgtatga ttatatgtta gtaaacactt tcaaatatga
     3961 aaagaggagc tattatggta ggaattaggg atgttgcaaa acgggcgggt gtgtccattg
     4021 caacagtatc gcgcgtgcta aatgaggatc cgacactttc agtaaccgat gaaaccaaac
     4081 gaaagattgc agaatcggtt aatttttatc attatgtcaa aaaaagtccc gttgcaaaaa
     4141 atttaacaaa tattgcatta ataacgacag tttctgaagt agatgagttg gaagatccct
     4201 attttcgaac gattcgcaga gggattcaag tcgaagcaga acaaagcaaa gtcaatttga
     4261 agaaaattat tcgtttgtca gaaacaccgt tggagcaaga cgatttgcta aaatgtcaag
     4321 gtgtgttgat tattgggcag gtgctatctt ctgtgattga agaagtatca gcaattaatt
     4381 cgaatgtcgt agtgattgat gaccctagta ttgaagctga agtagatgca gtttatactg
     4441 atttaacaaa agcaacgaat cagcatttag atcgattgta taaaaaagga caccgaaaca
     4501 ttgcgtttat cggaggagga cgtgttcgtt tagatagcca aggaaataaa cacgtgtcag
     4561 atgatgatta tcgaaaaata gcctatgaga cgtggatgaa acaaaaagga ttagaaaaat
     4621 atagtcaaat ttatttagaa ggctggacca cacttgacgg aatgcaggca gcagatttac
     4681 ttttgaaaaa acatgctaaa gataagttgc cgactgctat catggtagga aatgatccta
     4741 ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct acatattcca gaagatattt
     4801 cgattgtgag ttttgataat attgaagttg ctgaattttt aacacctgca ttaaatacgg
     4861 tgaatgtgaa tacagaagaa attggaaaac tagcagttcg catggttaat gaacgaataa
     4921 aagaagttag aaatgtttcg atcaaagtga ttgtttctaa taatattatt gttagagaaa
     4981 gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa gggtgtttac ttttttgttt
     5041 actaatattt ttagtaaaaa atctcgacaa aacattaagt aaacagttat ggtgttgtta
     5101 acaaaaagaa aacgcattca aaacaggagg aattaagatg aaacatttca aaaaaatagc
     5161 tttaacaggg atagctgttt taactgggac attactcgca gcatgtggtg ggggagatag
     5221 taattctaaa gacgggcaag taactttaag ctatgcgagt tgagatactg accaagctcc
     5281 aggactaaga aaaatgttag atgaatttga aaaacaaaat cctgatatca aagtagaaat
     5341 ggagacaacg ccttgggatc aatattgggt taaattagaa gctgcaacaa cgggaggtaa
     5401 tatgccagat gtagtaacca tgcactctag cgaatcctat aaatatatgt cacagaatgc
     5461 tttgatggga cttgatgatg ttatcaaaga aaatgatatt gacatgggaa actttactga
     5521 aggaattgct gatttctata cgcttgaaga cgaactatac gcaatcccta aagatgcttc
     5581 tgtagtgggg ctatggtaca acaaagaatt atttgataat gcaggtgtcg attatccaga
     5641 tgaaacatgg acatgggaaa cactcagaca agctgcaatt gatttaacag atgaatcaaa
     5701 aggcatctat ggttttgcat cacatattgg aacagagact ggctactggc catttattta
     5761 tcaaaatggg ggagaagtat ttgccgatga caatacaaaa tcaggtttga ataccccaga
     5821 agcacaagaa gctttagctt tttatcgaga tattgcactt gttgatcatg cttcaccaac
     5881 aattacagaa ttacaagatc ctgataagtt aagtcgtttt caatcaggta gattagcaat
     5941 gatgattgaa ggaaactggt ttgcaccggg atttttgaaa aatgactatc tagttgaaaa
     6001 tgttgcttct gccgtgttac ctaagggaaa agttaatgca acaattacaa atggacttgg
     6061 ttgggctgcc agtgcgagta caaaataccc agaagaagtg aaaaaactaa tggcattttt
     6121 agctagtgag gacgccaata aaatccaagc ggaaacgggg gcatcaatcc cagcactaaa
     6181 aggctttgaa gacgagtggg ctgcttcttc agatacaatt gatttgagtg cttttggaaa
     6241 aatgttggag tatggagtcc ctcgtccata caataaagat ggattacgag ctgaacaagc
     6301 agaagttcaa attattaacc gtattctgtc tggtgaagta actgttgaag atggaaccgc
     6361 tcaaattgaa aaagaagtca atgcgatatt gagcggaaat taaattagtt aattatacaa
     6421 tatacaggcg aaaggattct atttcgtctg tatattaatg gatgaatacc aaaaaggggg
     6481 atattatgaa aaatcgaatt tttggaaaat atcgtaaaga tcaagtgata tggggctata
     6541 tttttatttt tccaacggca ttaggattaa ttttattaaa tatatggcca gcaattcaaa
     6601 cagcaatttt aagttttcaa aaaacggtag ggttcggtga tactgaatgg gtcggattaa
     6661 gaaattacca aaaaatgttt gcagacggtg aagtttttca gtctttatta aacacattaa
     6721 tttatgcagt ggtttcgata cctagtattg ttgtattatc cttgttggtg gcagttttaa
     6781 tgaatagaaa agttaaagga ttgtcgattt atcggacaat ttattttcta ccagtcgttg
     6841 ctgcaccatc cgcagtcgca atgatttggc gctggatgtt caataatgat tatggactga
     6901 ttaataacat gttggctaaa ataggtctcg atggaccagc atggttaacg gatccatcga
     6961 ttgcgattta ttctattatc attgtaggag tttggagtgc tattggttat aacatggtgt
     7021 tattactagc cggcttataa gaaattccta aagattatta tgaagcagcg gaaattgatg
     7081 gtgctagtcc aatccagcag tttttcaaca ttacgttacc attagttaca ccaacattat
     7141 tttttgtagt tgtaacaaca gttatcaatg ctttccaagt atttgatgtt atttttatga
     7201 tgattactcc taatagtacc gccatgttta aatcacaatc attagcctat ttattctata
     7261 aacattcatt tattttaaat gacaagggat atggttcggc aatcgttatg ttccttttag
     7321 tgattatatt aattattact gcaattcaaa tgaagttgca gaaaaagtgg gtaaactatt
     7381 aaggaggcta tcaaattatg aaaaataaat ttttttcaaa tgcgccaatc catatcatgc
     7441 ttattttagg tggtattgca atggcattac cttttatttg gatgatttta acttcaggga
     7501 aaacgttggt tgaatctacc caaattccac ctacaatttt ccctgagaaa tttcagctaa
     7561 gtaactatca acaagtactt gatttattac cattcatggc tttttatatc aatacatttt
     7621 taatggttgt tggacgagtg attggctcgg ttttattttc agcaatggct gcatatgctt
     7681 gtgcgcgtct aagttttcca gggaaaaata tccttttcag cttagtactt attcaaatga
     7741 tgattcccgg ttccattttt attactccac agtatttatt agcacaaaaa ttgggtattt
     7801 taaatacaat tccagcatta atattaccag gggtagttag tgcgtttggt acgtttttat
     7861 taagacaatt tttcttaggt ttacctgatg aattggagga agcggcctat ttagaaggtg
     7921 ccaatatttg gacgatcttt ttcaaaatta tgttgccgct tgctaaatca ggattggttg
     7981 cacttggaat attcacagcg ttgtttgcat ttaaagattt aatgtggcca ttgattgtca
     8041 atacttcaat tgataagatg cctttagctc cgggattagc aaacttacaa ggacaatatg
     8101 ccacgaactt cccacaatta atggcaggtt cgttattagc aatttggcca atagttgtga
     8161 tattcatcgt tttccaaaga aaattcattg aaggaattgc tacaacaggt agtaaattat
     8221 aaaactatta attagtgatt ggagagtaag tacatgacaa aaattacatt tattggtgca
     8281 ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga tgttaactcc aagtctacaa
     8341 gatgcacata ttgcattgta cgatattgat gaaaaacgac taaaagaatc aaatttaatg
     8401 cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg atattgtttc ttattatgat
     8461 cgcaaagaag cgttaagaga ggctgatttt gttatcaatg ctattcaagt gggtggttat
     8521 aaaccttcaa cagtgattga ttttgatatt cctaaaaaat atgggttgcg acaaactatt
     8581 ggtgacacca tggggattgg tgggattttt agaaccctac ggacactacc agtaatgtta
     8641 gactttgcta aagaaatgga agaagtttgt ccaaatgctt ggttattgaa ctatacaaat
     8701 ccgatggcga tgttaaccat gggtgtttta aaagcgacga gtattaagac tgttggttta
     8761 tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc atttaggaat agccgatcaa
     8821 tatgacttaa atgactttca atggaaaatt gcaggcatta atcatatggc attcttgttg
     8881 gaaattacaa aaaatgggga agatttctat ccaacaattc gtcagttagc tgcagaaaaa
     8941 ccaaatccac atccagattc tgtacgtttt gaattgttga aacgttttgg ttactatatt
     9001 acagaatcaa gtgaacacaa tgcagaatac catccatact ttattaaatc aaaataccca
     9061 gaattgattg acgaattagg gattccgttg gatgaatact tacgtcgttg tgaacaacaa
     9121 attaatgatt gggaaagcat gcgagaggac attgtaaata atggggagtt aacacatacg
     9181 agaagccgtg aatatgcttc atatatcatg gattctatta cgacaggaac tccgtcagtg
     9241 attgcaggaa atgtcttaaa taaaggctta atcacaaact taccgacaga tgtttgtgtg
     9301 gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa cctatgtcgg tgaattgcca
     9361 acacaattag ctgctttaaa tcggacgaat atcaatgttc aagaactgac agtagaagcc
     9421 gctttaacac tagataaatc aaaaatttat caagcagcgt atttagatcc acatctatca
     9481 tctgaattgt ctttaacaga tatcaaaaat ttagtggatg atttaatcga agcacatggg
     9541 gactatttac ctgaatataa ttaaaaagaa gctgaaagag taaggaattt tcaattcctt
     9601 actcttttct tagttaaatt tagtaaaaat attgacaaaa cacttagtaa acagttacat
     9661 tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg aggagcataa aatgaagtgg
     9721 tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa cgatctcttt gactgcttgt
     9781 ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag agttttttaa tcaaaaaaaa
     9841 gaaatgactc aaacgattca ggagattgct aaagattttg aagcaaaaaa ccctgatatt
     9901 catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa ttaaaacacg tatgcttgca
     9961 ggcgatgtac cagatgtcat taatctttat cctcaaagta tagaattgaa agaatgggcg
    10021 aaagctggtt atttagagga cttaaccgaa gaaccgtatt tagagaatat caaaaatgga
    10081 tatgcacaac gttttgctat tgaggatcgt gtatatagca taccattaac cgctaatgtc
    10141 tatggttttt actataacaa aactgctttt gaggaaatgg ggattcaagc accagaaact
    10201 tgggcagaat ttgaaaaaat agtagctgag attaaagatc aaaacaaagt accgtttgcg
    10261 attgcaggag cagaaggctg gactttaaat ggttatcacc aactagctat agcgactgct
    10321 gctggtggtg aagaggaagc gaacaatgtt tggcgtttct cagaagttaa tggaattaat
    10381 gctgaaagca aagaaatgca aaaagatttt aatcgattag atttgttaag agaaccaaaa
    10441 gctttgcaaa acaactggca aggtgccggt tataacgata cagtagttac ttttacaaaa
    10501 ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc cgatgattca ttcacaaaat
    10561 ccagattttg aagtaggaac ttttcctttt ccagcagatg aagcaggtca aagtctaacg
    10621 attggcgcag gagacctagc cttatcagtt tctgcaactt ctcaacataa agaagaagcc
    10681 aaacgttttg tagaatatat gacaacacct gaagcgatgc agaaatatta tgatgtagat
    10741 ggtgcaccgt gtgctgttga gggcgttatc gagaatactc aggattcacc attaagtggt
    10801 ttaactgaac ttgcttttac tgatcgtcat ttagtttggt tagccaaaga ttggaatagt
    10861 gaaaatgatt tttatacctt aacaacaaat tatttacaca atggcaatca aaaagccatg
    10921 attaacgcat taaatgcttt ctttaatcca atgaaagctg acggaaggta ggaaaataaa
    10981 atgaaaataa aagaaatttt aaatcgttac tgggcaatca tttttgtagc aattccaatt
    11041 attttgcaga tgattttttt ctactttcca atggttcaag gtgcttttta cagtttaact
    11101 aattggactg gtctgactta taattttgat tttgtgggtt tgaacaatta taagattctt
    11161 ttttctgatc cgaagtttat caaatcgatt ggcttcactt taattgtgac gctttgcatg
    11221 attgctggac aaattggttt tggtattttg attgcgcggg cgttgaacgc aaatattaaa
    11281 gggaaaacat tctttagagc atggttcttt tttccagcgg tcttatcggg cgtggttgtt
    11341 tctttagtat ttaaacaaat ttttaactat ggtttaccaa aaattggtag tatgttaggc
    11401 attgaatttt tacaaaccag tcttttagga acaacatggg gagcgatttt tgcagtaatt
    11461 tttgttttac tttggcaagg aattgccatg ccagtcatca ttttcttagc gggttttcaa
    11521 agtattccac aagaaattat tgaagctgct agaatggatg gggcgaatga taagcaaatt
    11581 ttttggaata ttgaaatcac ctacttatta ccaagtattt caatggtatt tattatggca
    11641 ttaaaaggtg gcttaacagc atttgatcaa atttttgctt taactggtgg tggaccaagc
    11701 gatgccacaa catctttggg gttacttgtt tataattatg cttttaaaaa caatcagttt
    11761 ggttatgcga atgctattgc tttagtctta tttatcttaa ttgggattgt ttcactggca
    11821 caattaaaat tatcgaaaaa atttgaaatc taggaggttt cccaatgaaa acatcaaaag
    11881 aaaaaaatat ttctaaatac ttgcttttat tattaggggg cttcatgatt ttagttccct
    11941 tagtttcaac gctattcggt tcatttaaaa caacacccga tattatgaat aatttctttc
    12001 gttttcctaa tccagcgact ttggaaaatt tccgaagatt gttagcggat ggaattggtc
    12061 attacttttg gaattcggca atcatcacaa tcattgcagt agttgctgtg acagcaatta
    12121 ttcctatggc tgcttattca ctagcaagaa aaatgactcg tgggaaagca tacggattga
    12181 tgtatttatt tttaatttta ggaatttttg ttcctttcca agtaattatg attccgatta
    12241 ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg attaatctta ctctatctca
    12301 cttatgccgt accacagaca ctatttttat atgttggttt tattaaacta aatgtgccta
    12361 tcagtctgga tgaggctgct gaaattgatg gggctagtca ctttacaact tatttcaaaa
    12421 ttgttttccc aatgttaaaa ccaatgcatg cgacgactat tattcttaat gctctatggt
    12481 tttggaatga tttcatgcta ccattattaa ttttaaatcg tgattctaat atgtggacac
    12541 taccattgtt tcaatataat tatacgggac aatattttaa cgactatggt ccaagttttg
    12601 cttcctatac agtaggaatt gtcacgatta cactagttta tcttgctttc caaagaagta
    12661 ttatagctgg aatgagcaat ggagcagtga aataaccaat aaattattct cattataaag
    12721 gagttcataa acttatgatt atatttgaca atcacacaca aacatttcat ttacaaaata
    12781 aaaaaattag ctatctatta ggaatcgaag aaaataatta tttaacgcat ctttattttg
    12841 gaaaaaagat tacgcactat tctggtgggt atcgctaccc ccgaacagat cgttccttct
    12901 cacccaacaa tgcaaatgcg caagatcgtt tattatcttt agacacatta ccattagaat
    12961 ttccaggata tggacatggt gattttagag aaccggcgca taatattaaa ttaggaaacg
    13021 gtagtcgtgt aaatgatttt cgttatgata gctacgaaat tcaaaaagga aagcccagct
    13081 tagcgggatt gccagcctta tatacggaag atgatgatga agctgaaacg ctgattattc
    13141 aattgaaaga cgcgattagt ggattgactt tacagttgtt ctacacaatt tatcaaaatc
    13201 acagtgtaat tattcgttct acgcaattag taaataatag tgatgaaaca gtggagatta
    13261 accgcttagc tagtcaatca attgattttc caaatcgtcc actcgagtta attcatttaa
    13321 atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa aattcataca gggataaaag
    13381 tattagatag taaacgcgga tcaagcagcc atcaacaaaa tccgtttgtt gctttagtag
    13441 atgccaaaac agatgaattt caaggagagg catatggatt taatttagtt tatagcggta
    13501 atcacgaaac ggtgattcaa aaagatccat ttgaccaaac acgggtcatt atgggaatta
    13561 attcatttaa ttttggttgg caactaaaaa ctggcgactc atttcaaacg ccagaagtcg
    13621 tgatggttta tagtaatgaa ggtctgaata cgttatcaca gacgtatcat gaattgtata
    13681 atcaccgttt aattcgtgga aactatcgtt taaaagaacg tcccacgtta atcaacaact
    13741 gggaagcaac gtattttgat tttaacgaag aaaaaatcat gaatattgtc gatgatgcga
    13801 agaacttggg cattgaaatg tttgttttag atgatggttg gtttggcggt cgtgaaaatg
    13861 acttcacttc attaggtgat tgggttgaaa cagaaggcaa acttgcttgt ggtttggaaa
    13921 atttagccaa gcaagtgcat gataacggga tgaaatttgg gatttggttt gaaccggaaa
    13981 tgatttctga agatagtgaa ctatttcgag cacatccaga ttgggcattg ggtattccag
    14041 gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga tttttcaaga caagatgttc
    14101 gtgataatat ctatcaacaa atgacggcga tttttgatcg tgtaccgatt gattatgtga
    14161 agtgggatat gaatcgaaat atgacagaag tctattctgt cttgttggac tcaacgatgc
    14221 aaggtgaagt ggctcatcgt tatatgttag ggttatatga atttttagaa aagttaacaa
    14281 cgaattatcc aactatttta tttgaaagtt gttcgggtgg aggcggtcgt tttgatgcag
    14341 gtttcttgta ttacatgcca caaacttgga cgagcgacaa taccgatgcc gtggctcgtc
    14401 tgaaaattca atatggaacc agcttagtgt atccaatttc aagtatgggt gcgcatgttt
    14461 cagcagtccc aaatcatcaa acaggaagag aaacgagttt aactattcgt ggcaatgctg
    14521 cgatggctgg tgtttttggt tatgagctag atctaacgca attaacttct gaagaaaaag
    14581 cagaaattaa agaacaagtt gccttttata aaaagcatcg ccaacttttg caatatggaa
    14641 ctttttatcg cttggaaagt ccgtttgaag gcaatgatac tgcatggatg tttgtctcaa
    14701 aagaccaaac agaagccatg gtcttttatt tccgagtatt agcagaagct gcgcatccat
    14761 tagtcacatt gaaattagca ggattagcag aagaagcaac ttatcaaatg gaagatgtaa
    14821 catttgctgg cgatgaattg atgaatctcg gtttttatat tgactcagaa ctacatggtg
    14881 attatgcgac acaacgcttt tatttgaaac agatatagta gcttgaattt acaatataag
    14941 tgcaacacca aaagagactc attccaaacg aagaaaatat gttttattta cacgcattta
    15001 ctaaaaagca gcctgatttg aattgggaaa atgaagaagt acgtgaagaa atctatgcaa
    15061 tgattaacta ttggttagat aaaggactag gcggatttag aatcgatgcg atattaaaca
    15121 tcaaaaaaaa actagagtat ggcatctttg aatctgatgg agaagatggg ttagccttca
    15181 ttgggccctg gattcttaat cagtcgggca ttgaagtgtg gttaaaagaa atgagagagc
    15241 ggacatttaa accacataat agtatgacgg tagcagaagc cgatgttcct aatgaacgtt
    15301 tagatgaata tattggtgaa gatggctatt attcgatggt ttttgatttt agttatacgg
    15361 atattgatgt acctgaaact ggtgaatggt ttaaagatag ccaatggaca tggacggata
    15421 tgcgtaccaa tatttttacg aatcaattag ttacgcaaga taaaggttgg ggcgccttat
    15481 atttagaaaa ccacgatcaa ccacgttcaa ttaataaata tattccagaa gaatggatca
    15541 atgattatag taaaaaaatg ttggcgacgc tttttatgtt actaagaggt acccctttta
    15601 tttatcaagg acaagaacta ggaatgacca acatagaaat ggcatcatta gaagattttg
    15661 atgatgtagc tacgcattct cagtataaac gggcattaga atatggattg gcaccagagc
    15721 aagcattgaa agctgtcaat aaacgaagtc gggataattc tcgcacacct atgcaatgga
    15781 atacgcaaaa aaatgccaga ttctcaactt ctgataatgt atggttaaaa gtaaatccta
    15841 attatccaga actcaatgca gaagctcagt taaccaatca acagtcggta ttcaattatt
    15901 atcatcaatt aattaaatta agaaaaaata gtgtatacaa agacgtgcta atctacggac
    15961 aatttctacc agtgaaagat aaaaatgaac atattttgct ttatgaacgc caattagaag
    16021 ataaaatcgt attagttatc ttaaacgtta cagcaacaga acaagaatat tatgtagatg
    16081 aacagtacca acaagtatta atcaataatt atgaagatgt tttattagat caaagtcgaa
    16141 aattgcgttt acgaccgttt gaaagtattg tattagctaa ttatggagga caattatgaa
    16201 aattaaaaac gaagcaatgc tcatcaccta tgcagatagt ttaggaaaaa acatgggcga
    16261 attaaaacaa gtcttagaca aacatttaca aggtgttatt ggtggtgtac atctgttgcc
    16321 tttttttcca tcgacaggtg atcgaggctt tgcgccgagt gattatacga cagttgatcc
    16381 agctcttgga acatgggaag atgtggaagc attaggtgaa gattattatt tgatgtttga
    16441 ttttatgatt aatcacattt ctcgtgaatc aaaatttttc caagatttca aaaaaaacca
    16501 tgagcagtca ccatacaaag aaatgtttat ccgtattcat gaatttttcc cagaaaatcg
    16561 accaacacaa gaagatattg atttaatcta caagcgtaaa gacaaagcgc cgtttcaaac
    16621 ggtagaattt gcggatggta cgacagaaga ggtttggaat acctttgggg aagaacaaat
    16681 tgatttagat gtgaccaaag aagttgtgaa agaatttatt cgagaaacca tcaaagatat
    16741 ggcgaaccat ggctgttctt taattcgatt agatgcgttt gcttatgcga ttaaaaaatt
    16801 agataccaat gatttcttcg tggaaccaga tatttgggaa cttttagacg aagtgcgtca
    16861 agaagcagcg aaatatgacg tcgaattatt accagaaatc catgagcatt attccattca
    16921 aatgaaaatt gccaatcatg attattatat ctatgacttc gccttgccaa tggtcactct
    16981 gtattcactt tatagtggaa aatcagaacg tttagccaat tggttgaaaa tgagtccgat
    17041 gaagcagttt acaacattgg atacgcatga tggaattggt gttgtggacg ctcgtgattt
    17101 attaacagat gaagaattgg attacacctc tgaggaattg tataaagtcg gggcgaatgt
    17161 gaagaaggtc tattcaagtg ccaattacaa taatttagat atttatcaaa ttaacagcac
    17221 ctattatagt gcattaggcg ataatgaccg tagctatctg ttagcacgtg cgattcaagt
    17281 atttgcgcca gggattccac aaatttatta cgttgggtta ttagcaggaa aaaatgatat
    17341 tgaactgttg gaagcaacga aagaagggcg taatattaat cgtcattact atgatttagc
    17401 cgaaattgaa gaagaagtgc aacggccagt gattcaacaa ttatttgcac ttttaaaatt
    17461 tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt gaagtagcaa cgccatcaga
    17521 atcagaaata gtgattacac gtaaaaataa aaacggtagt gatagtgcaa cattaacggc
    17581 taacttggat acgaaagaat ttatcattga gtctaaagat ataaaaattt tcactagtgt
    17641 tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc tttatactag cacttggatg
    17701 acttatccaa gaccaataaa aaggactcat acatggataa gtataaaaca aatacagcat
    17761 ttaataaatg gttttctgcc ataaaattag gaaaactttc tgtcaactcc caaaaatgta
    17821 tttttgatta caattcgtat cgtaaaaaac tgtcttttga agctgtgctc aagctctttc
    17881 tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt gagtacagct cttatcgacc
    17941 gatcgctcca actagaaacc gatgtgtcat cgattagtca tacccaattg tctcgagcat
    18001 ttaatgcact tgaacctaaa gtgttagaag aaatttttca acaactgtta gaaaaagttc
    18061 aacatcaaat caaaccaacc aagcgaaatc atctgtatct gattgattcc agtacgtttt
    18121 cgcttagttt aaaacgtcat aagtgggcga ctttccgtca aacaaaatca ggtgtcaagt
    18181 tacacttaaa ctattgttat atggacgata ctacgatgta tccaactgat tttaccttaa
    18241 caaatgccca tgaacatgat ctcaatcaac taccagtatt ggtgaatcaa ccagaggcaa
    18301 cttacgtgtt tgatcgtggg tattaggact ttgaaaagat ggatcagatg cattgggatg
    18361 gctatttctt cgtcactcgg attaaaaaaa acaccaaagt tcacgtgata gatactctgg
    18421 aaacatcgcc agaaacagaa attctacgag acgagctggt tcgtttagga tctaaaacct
    18481 atctaaccgc caatttccga ctagtcactg ttcaagataa gaacggacgt gtgtttcaat
    18541 ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc tgatatgtat cacgctcgtt
    18601 ggcaaatcga attgtttttc aagcacatca aacaacacat gaccattaaa accttctttt
    18661 ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac gatgatttcc gctctcctca
    18721 cgtttctcat caaattggag acgaaaacgg aaaaatcagt gttccaaatc aaacgattct
    18781 ttcgttattt actgtttcag ccctttgaat gttgtctaga aaaattaatt cccacatgag
    18841 aaaaaagaaa tttggctcgt tacttttggt tttcagttga ataacagata aaaaattttc
    18901 tggaaaaagt catcctctgc gtcagcacag ttttttttga cttattttca gaggaaatag
    18961 ttttcagaat attctaaaat gttttatgca acactagtga tattttttag aattagattt
    19021 ctaatgtgca ttttttgagt attaaggagc aagtatacaa agatttataa agctgattaa
    19081 atttgaataa aaatatgcag gaacgctact atttttagta gtgttctttt ttatatttga
    19141 ataaaaaatg tggtggttaa atggtggtta taacaaaaaa tgaattaatg aaattaggtt
    19201 atgggaagta tcagtctgag agaatcatac gacaggcaaa agcaattatg gtttcaaaaa
    19261 gctataccta ttacgaaaat agaagattag gactagtacc tgttgaatct gtagaagaag
    19321 ttttaggtat gaagatagag gtagaggagg attttcagaa tcatggttaa ttttaaaaaa
    19381 gataaaaatg ggaaatatca ttttactgct agtttgggat tcgacaaacg aacagggaaa
    19441 agagtacaaa aaaacgtaga ggggttaaaa cagttaaaga agaaaaaaag cgtataccga
    19501 acttgtttct aattatggaa aagaagcaga aacttctcaa agtactatgt tgtttgagga
    19561 gtttttttat aaattattcc taccttatta tcaagggaaa gtagaagaaa gaacatataa
    19621 aaatcggtta tctacaatca aaaaaacttt tcttatttct ttaaaatgga gttaagagaa
    19681 atacgtccaa ttgacattca aaagtggcaa aataatttat tgagcaaata taaaaattct
    19741 tatgtaagaa acatgtatgg gatttttcag atgtcattag atcgagcagt tgttttggga
    19801 ctacttgaaa aaaatcctgc aaaagttatt ggaaatgtga agaaaaggaa aactacagtt
    19861 gatttttgga caaaagaaga atttacaaaa gttatttcaa ctttttatat tgatgatttg
    19921 taccaacatt ttggttttat ttgtatgtgg ttacttttta tgaccgttat gcgtttggga
    19981 gaagccactg cattaacttg gaaagatatt gattttgaaa ataaaaactt gcgtgtgaat
    20041 aaaacactct attataagag agccaaaacc tatgagtttg tagaacccga aacggcttct
    20101 agtattcgta caatcgaact agacgacaat acaattacct ttttaaaagc ttggaaggag
    20161 cgtcaagttt caataggagg catgtcgttt gtactttctt ataatggaac acctactcag
    20221 aaatatacaa tccgtcatat tatgagaaga catgcaacga tcgttggtgt tcatataatc
    20281 cgcatacacg gattaaggca tagtcatgct tctttgctta tatcacaata aaaagatgca
    20341 cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa cgactttagg aacttatggt
    20401 catttgtatc taaattcatt gagagagata acaaatgatc ccacagatta tatccaaact
    20461 aatttttcta aaatcaaacc gacacctatt tacgataata aaaaataaat gataaacaaa
    20521 agtaggatca caccaaaaga aacacactag aaaaactata atacaggctt ttctagcgtg
    20581 ttgtttctta tttccgccta tttatacatt atactcataa agcatttaat gcatcgatcg
    20641 tagccgcatt gtctatatgg gtatataaag ttgttgtatt ggtacttgaa tgtcccaatt
    20701 gttgggcagt taataccaat gaatttgtct gttggtagag tctagtagct aatgtatgac
    20761 gcagtttgtg agggctgact tgaattctaa agccttttga atattttgct accatccgtt
    20821 cgattgcaat accggatata cgcttggctt ctccttggta aattgctaga aataaagctt
    20881 tttcgttttc tgttacttta tatcgttctt tccgaatagc tagataatta gctaaatatt
    20941 ccattgcgaa tgctgctata ttgacagcat ctcttttatt tccttttcta gttactacca
    21001 cattattctt atctaaagat aggtcatcta aattaatatt tataacctcg gagagtctca
    21061 atccagatcc taaaatcagt gcattgatcg ccaggtctct ttctacatct cgttgataat
    21121 agatcaaagc tcgtttactc aaagtatttt tatattcatt ggcaatataa tctagataat
    21181 tttgtgtatc cttatctaga aataattttt tctcaatggc attggcacgt gcagctaacg
    21241 tagttttatc tgtgatggtt gctacttgat ccataggatt attgtctaag tatggttttc
    21301 ctagaggatt attggaactt ttcgaaagat atttaaatag aacctttaat gccgtgatat
    21361 ttctattgat agtagaagta gatagaccta agttattatt atctttgagc ttagtttttg
    21421 ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa atgagatttg tacaagttta
    21481 ggtcataggc agttaactgt cttatcgagg tgatagaagt atcaggaacg atagactcac
    21541 tcagaagcca ttcataaaag agcttataat tttttagata ttctaaagtg gttcgaggtg
    21601 attgatcttt atcctcacga tcttgaacaa aatcttttat ataatcgggc atatgaagta
    21661 tgattgtatc tattttttga tatatttctg tatttt
//
LOCUS       DAHUWM010000025        21135 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHUWM010000025 DAHUWM010000000
VERSION     DAHUWM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.025"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..1056)
                     /locus_tag="NK788_000666"
     CDS             complement(<1..1056)
                     /locus_tag="NK788_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6769014.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHL"
     gene            complement(1159..1599)
                     /locus_tag="NK788_000667"
     CDS             complement(1159..1599)
                     /locus_tag="NK788_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6769015.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(1656..2066)
                     /locus_tag="NK788_000668"
     CDS             complement(1656..2066)
                     /locus_tag="NK788_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCH6769016.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2246..2680)
                     /locus_tag="NK788_000669"
     CDS             complement(2246..2680)
                     /locus_tag="NK788_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6769017.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(2780..3628)
                     /locus_tag="NK788_000670"
     CDS             complement(2780..3628)
                     /locus_tag="NK788_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6769018.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4017..5783
                     /locus_tag="NK788_000671"
     CDS             4017..5783
                     /locus_tag="NK788_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCH6769019.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            5885..7144
                     /locus_tag="NK788_000672"
     CDS             5885..7144
                     /locus_tag="NK788_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6769020.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7291..8592
                     /locus_tag="NK788_000673"
     CDS             7291..8592
                     /locus_tag="NK788_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769021.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            8595..9446
                     /locus_tag="NK788_000674"
     CDS             8595..9446
                     /locus_tag="NK788_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769022.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9459..10274
                     /locus_tag="NK788_000675"
     CDS             9459..10274
                     /locus_tag="NK788_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCH6769023.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10425..11591
                     /locus_tag="NK788_000676"
     CDS             10425..11591
                     /locus_tag="NK788_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6769024.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            11821..12468
                     /locus_tag="NK788_000677"
     CDS             11821..12468
                     /locus_tag="NK788_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCH6769025.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            12552..13496
                     /locus_tag="NK788_000678"
     CDS             12552..13496
                     /locus_tag="NK788_000678"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCH6769026.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            13631..15082
                     /locus_tag="NK788_000679"
     CDS             13631..15082
                     /locus_tag="NK788_000679"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCH6769027.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15103..15939
                     /locus_tag="NK788_000680"
     CDS             15103..15939
                     /locus_tag="NK788_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCH6769028.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            15939..16598
                     /locus_tag="NK788_000681"
     CDS             15939..16598
                     /locus_tag="NK788_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6769029.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            16724..17413
                     /locus_tag="NK788_000682"
     CDS             16724..17413
                     /locus_tag="NK788_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769030.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17419..18375
                     /locus_tag="NK788_000683"
     CDS             17419..18375
                     /locus_tag="NK788_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCH6769031.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18419..18931
                     /locus_tag="NK788_000684"
     CDS             18419..18931
                     /locus_tag="NK788_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769032.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19187..19735)
                     /locus_tag="NK788_000685"
     CDS             complement(19187..19735)
                     /locus_tag="NK788_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6769033.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(19824..21005)
                     /locus_tag="NK788_000686"
     CDS             complement(19824..21005)
                     /locus_tag="NK788_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCH6769034.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         7030 a         3804 c         4088 g         6213 t
ORIGIN      
        1 aagatggttc gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat
       61 gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt
      121 ataaaaaatc ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc
      181 aattagatgg acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg
      241 ggaataagct atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag
      301 acttaaaatg aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa
      361 ggcgattgta gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa
      421 accaaatgta ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa
      481 ttcacttttt aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa
      541 gaaacttaag atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata
      601 gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata
      661 agtatggata tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat
      721 aacggactgg atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc
      781 cagatagata aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat
      841 tcggacaaat cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt
      901 gagcaaaatg gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa
      961 aacagtgaga cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac
     1021 aacctccaaa taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag
     1081 aaagagcaaa aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa
     1141 atcgttcatc tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt
     1201 tcgggtcaga tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat
     1261 cttttgtgga ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac
     1321 atttacgatt ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa
     1381 cttttttagt aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt
     1441 cactgaagtc tttttctttt cccataaaac taacatcaca agatactggg tataggtaag
     1501 attcagcggc ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa
     1561 aggaaaacat acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta
     1621 ctatagtgta tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta
     1681 acttctatat ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat
     1741 aattcttgcg tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag
     1801 acaaagctcg tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca
     1861 gagatatttt tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca
     1921 gctgcaaaca attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata
     1981 ttcaattcga atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc
     2041 attttcgtag aatacatttt tttcataaag ttaccacctt taattatatt ttatacaatt
     2101 gaattttaca caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga
     2161 gaaagcacat gaaacagacg cgctttctcg aatagcaaac atatacaaaa aatatcagag
     2221 acaaaaaata atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt
     2281 caaaaattac ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga
     2341 acattgagtt agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc
     2401 catttcgtaa tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga
     2461 tcgtatttgg atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca
     2521 tatggaatct cgtaccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg
     2581 ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac
     2641 tatcatttaa tacaaaatcg atttccgttc ctttatgcat aaagatctcc tctccttttc
     2701 agtcgaacag ttactagatt cttttattat accagctaaa gaaaacgcat tcaaaaaaat
     2761 agcggattta cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt
     2821 ctgctacgct gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca
     2881 ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat
     2941 acccgccttt tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc
     3001 ggatgtcttc caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg
     3061 tttcgatata agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact
     3121 cttctattcc aaaaataaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc
     3181 caatcgaagt attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc
     3241 cgctagaaga gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa
     3301 aaatgacaga caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta
     3361 aaggaaaaac caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa
     3421 gagctagtac caacataacg atattcaaca ctaaaataac tatagctcgg tcaaagccta
     3481 aagcagcttc cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc
     3541 ctaaaaacat attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg
     3601 tccatataaa ttgtctcgtt tctttcattt attcacttcc ttaattcatt aataatactc
     3661 ttatcttatt ataacttact tttcacctta agttaacttt aattaataaa aaaattaggc
     3721 tagtctaaat tattatgttt cattcttcta gaatatactt ctacgccaaa aactgttaaa
     3781 aaacttttac tttagaaaat tggagaaatt gatcgcttaa gataaattca ggacactaaa
     3841 tagtacagga agtagactaa aacttttaaa agttttaagt ctatctattt ggcaagttta
     3901 aaaaataaaa attcggtatc aaaaaactgt taccgataac atttttgaaa gcgttgtctt
     3961 ttgcttctct atcacttata gtgatgacgt catattaaaa cacaaggagg aaatcaatgg
     4021 atacagctgc aatttatcat cgaccggaaa gtgaatatgc atttctatat acgaatacaa
     4081 gatttcgtat tcggcttcgt actaggaaaa atgatatcaa aaaagtatat gtgctatgtg
     4141 gagatccata taccataaca actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat
     4201 gtctaagtac caatgttcat gattattggg aaattgaagt gacgagcgaa acaagtcgct
     4261 tacagtatgc gttccatgtt gttggggaag ataacacgga ctgtttttac ggtgatcaag
     4321 gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt cgaatacctt
     4381 attttcatca aatcgatcga ttcactattc cagaatgggt aaaagaaaca gtatggtatc
     4441 aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa cacactttac
     4501 cttggggaag taaagatcca gaccgagaag actttttcgg gggagatttg caaggtgtga
     4561 tggatcattt ggattacttg acggaccttg gcatcaatgg catctatttt tgtcccatat
     4621 ttaaagccac atccaatcat aaatacgata cgattgatta ttatgaggtg gatcccgctt
     4681 ttggcgataa aaaactttta aaaaacttga ttgatgaagc acataaaaga gggatacgta
     4741 tcatgttcga tgctgtattc aatcatatgg gtgtccattc accacagtgg caagacgttc
     4801 tcaaaaacgg agaaaaatcg atctataaag actggttcca catccgtttt ttcccagtag
     4861 attcttatca gatgactgac ttaccagaaa ctgctgaaaa tatcccatat gatacttttg
     4921 cttttactcc attcatgccg aaactaaata cagcaaatcc agaagtgcaa aaatatttgc
     4981 tggatatcgc aacttattgg ataaaagaat tcgatattga tggatggcgt cttgatgttg
     5041 ctaatgaagt cgatcatcat ttttggaaaa aattcagaga agcagttaca gaaatcaaac
     5101 cagacatcta tatccttgga gagatttggc attcctctca agcttggctg caaggggatg
     5161 aattccatgc agtgatgaat tatgcattta cagattcgat taaagattat tttgcaaaaa
     5221 agaaaatcac agcttcacaa atggtcagtg gaatgaatca tcagcaaatg ctttacagag
     5281 atcaggtgaa cgaaggaaca ttcaacttat tagattctca tgatactgcc aggatcttga
     5341 ccctttgcca aggtaacaaa gaattgatga agtctgtgat ggcctttatg tttcttcaaa
     5401 aagggtctcc ttgtatctat tacgggacag aaatagggat gactggtgaa gatgacccgg
     5461 attgccgcaa atgtatgata tgggagaagg aggaacaaga tctagagcta ttcggtttta
     5521 taaaagaact tgtttctcta aggaaacagt tgtctaagat tatttctgaa ggttctactc
     5581 agtggctaat agtaaatgat cgtgaagata agctctattt cacacggaaa ctagagggac
     5641 agatcatcta tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg
     5701 aggtgattct tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag atacgacaat
     5761 acggattttt gatcatgtgt tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa
     5821 tgaagatttg tgcccattat aataagggtg taacagcacg ttgaatacaa aggaggaaat
     5881 gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg ttaatctctt taggaatctt
     5941 aggcggatta gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg
     6001 aaaaacgtta acagtttccg ttgattctgg atataaagat tatataaacg aaataaaaaa
     6061 cactttcgaa aaagaaaacg atgtgaaaat caagctagta gaaaaggaca tgttcgatca
     6121 gttggaatca ttagcgttag atgggcctgc tggaaaggga cctgacgtta tgatggcagc
     6181 atatgaccgg atcggtgcgt taggacagca aggacatctg gcggaagtaa aactgggaaa
     6241 tgaagcagct tacgatgaaa cagacaaagc ccaagtgact tatgatggaa aaatctacgg
     6301 agaacctgca gtaatcgaga cccttgtttt gtactataac aaagatttag tcgatacagc
     6361 accatcaacg tttaaagatt tggagaatct atccaaagac agtcgttttg cttttgagtc
     6421 agaagcagga aaaaatacag gatttttagc taaatggact gacttttatt attcctatgg
     6481 tttaattgca ggttacggtg gctatgtatt tggtgatgat ggcacaaatc cttcagatat
     6541 cggtttgaat aatgcaggtg ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa
     6601 tgtttggcca aagggaatgc aagacataaa aagtgcagga gacttcgcca gccagcaatt
     6661 catgtccaat aaaactgcag caatcattga cggtccatgg caagcgcaaa catacaaaga
     6721 aaacaatatc aattatggtg tagccaaaat tcctactcta aataatggac agccttatca
     6781 accgtttggc ggaggaaaag gatgggtagt cagcaattat gctaaaaaca aagacctctc
     6841 tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt
     6901 aaatgaaatc cctgcaaacc aacaagctag ggaaacagca aaggctaaaa acgatgaatt
     6961 aacaacagct gtgattgaac aatatgaaac agctcaagcg atgccaaaca tcccagaaat
     7021 gggcgaagtt tggacaggtg cagaaaactt gatgttcgat gcagcatcag gttcaaaaac
     7081 accaaaagaa tccgcagatg aagcagtaaa aacaatcagt gaagccatag aacaaaaata
     7141 ctgattaaaa tgataaccgt ccatttttat aaaaaatgga acaggtgctt cagataaaag
     7201 tggcaagagc gagatggaaa attatcttgc tcttgttgcc attatcttaa aagcagtttg
     7261 cggtttagca agtttagagg gggcattcag atgcagcaag ataaaacaag caatatcaac
     7321 gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg ggcagtttgt aaatggccaa
     7381 gcttttaaag gtatcgtttt tttcgccata ttcgtcctat tcattggtca gatgcttctg
     7441 ggcggatttc atgcattggt aggattggtt actttgggaa gtataccaat ggaagatcat
     7501 tcattattta ttttgattga aggcactttg caattcatca ttacaatcat tttcttgatt
     7561 ttttacggtt tgaatctttt agacgcttat cgagtagcga agatgcgtca agagggcaaa
     7621 aaaatcaatc ggacgatgaa ggaagtcata cataatatag gtgatgaagg ttttccttat
     7681 ctattgacac ttcccgctta tcttctcatg atttttacga tcattttccc agtcctagtt
     7741 acattgttca ctgcatttac taattatgac ttcaatcata ttccaccagc cagtttaatt
     7801 gattgggtag gggctaaaaa cttttttagt atcttctttt taagctcata tcgtaatact
     7861 ttttttgcag tcttttcgtg gaccgtgatt tggactttat gtgcaacaac tttgcaaatc
     7921 acccttggta ttctcacagc ggttgttgct caccagaaat ttattaaatt caaacgtatt
     7981 ttcggtatca ttttcttatt accatgggcg gtcccagctt ttattactat catgagtttt
     8041 tcgaatatat tcaacgatag cgtaggtgcc atcaatactc aagtgattcc tttcataaac
     8101 cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa
     8161 gtagcaatta tcttagtgca gggctggtta ggatttccct atatctatgt gatggttaca
     8221 ggaatcttac aatcgatttc agaagactta tatgaagcag caaaaatcga cggagcaaat
     8281 gcatggcagc gcttttcaaa catcacgctt cctgctgtct tcctagtggc agcgccaaca
     8341 tttattacac agtatacggg gaatttcaat aatttctcca tgatctatct gtttaacaat
     8401 ggcggtcccg gaagcgtagg cggaggagct ggttctactg atattcttat ttcctggatc
     8461 tataaactta cgactggaac ttctccgcag tattctatgg cttcagcaat cacgttgatt
     8521 atctccgtca tcgttatctg tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat
     8581 atgggggatt aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa cgactttctc
     8641 acttttttac ttactttttt cttttaatac tgtcagtcat tatcatctat ccgcttttga
     8701 tcacagcaag tactgccttc aagtcaggaa atattgcagc ttttagttta gatctttcaa
     8761 gtgactggac tttaggaaac tttactcggc tattcagtga aacactttac gggacttggt
     8821 ataaaaatac attgatcatt tctgtcctaa caatgatcgt acaagtgact gtagtaactt
     8881 tggcgggcta tacttatagt cgctatcgtt ttatcggccg caaatcaagt ttgatgttct
     8941 ttttagtgat tcagatggta ccgactatgg cagcattgac tgccttttat gtcatggctt
     9001 tcttattaga agcgctcgat aagtactggt tcttgacaat gatctatata ggcggtggaa
     9061 ttccaatgaa tacttggctg atgaaaggat actttgacac tgtaccaata gatttagatg
     9121 aatctgctaa acttgatggg gcagggcatt tcaggatttt tttccagatt gtattaccgc
     9181 ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt tatgggacct tttggggatt
     9241 tcatgttagc taagttcctg cttcgaacac ctgaaaatca gactgttgct gtcggcttac
     9301 aaacatttat ttctgatgtg acaaaccaaa gagtctctct ttttgctgct ggggcaattt
     9361 tgattgcact accgatttgt ctattattct tctttttaca aaagaacttt gttaccggcc
     9421 tgacatctgg cggaacaaaa ggataggagc agagtatcat gcaaacattt ccgttaaact
     9481 atttttccgc attacgtacg ccaactcaag tgtttgcggg acgcaaacta ttatcatggc
     9541 ctaaattttt cttgatattt gtatttttag tttccttgat ggtcatgcct gtgactcttt
     9601 tttatgccaa tcagatccaa gcgattccat tagaacaatt tctatccgtt cattcgctga
     9661 tcgatgaaca aggaacccaa aaattttctg aacttgaatt atctgaaact gggttacaag
     9721 catcgcaaca aacgatagct gtgacccctg aaattttggt aggtgtgtct ttatctgaga
     9781 aacaacaatc cgatcatgga acttttattg attttgagaa ggaacaatgg gtgatccaac
     9841 aaaaggataa atctggtata cgccgctata cgatgaatta tagcccatct ttccaaccag
     9901 attctgtacg tacgcccgaa gatttccaac gatttttaga aagagaattt tatgccagta
     9961 atcgtccaac aatcatttta tcttatagtt taagtcttgg tcttgtccta tttgtcatga
    10021 cgagtctcat tttattcggg gcttcctttt tcttgtggat gacaagaaaa agccaacttt
    10081 catccattca tacattcaaa gagagtgcaa atttgatgct gaatgttatg ggaataggtt
    10141 ccatggcagc agcagttgta gggtttatcc actttgactt tatcttgatg ctaagtgtcc
    10201 aaacaacgat aactgtcttg ctgcttttat gggtttttgt caaaacaaga ttcaaagata
    10261 agcgtgtcga atgacaagtt taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa
    10321 ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt cttagttgat tcttttcttt
    10381 tttagtatta acgtaatagg gtagtttttt taaggaggaa aatcatgtta gagaaaactt
    10441 tatacaatca attattttca cgttctttct cgctccctgt cgaagtaaat tattgggacg
    10501 gtacaacgaa acaatacgga gatacggaca ctcctcccca aatcaaaatc aatatccatg
    10561 aagaaatccc aatgaaagag attacaagta acgcttcttt ggcactagga gaagcctata
    10621 tggatcatcg aatcgagatc gagggaagta tccaagcatt gatcaacgat gcttatcagc
    10681 aagcaaacag ctttttacga ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac
    10741 atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt aacgactttt
    10801 ataaactttg gctagatcca actttgactt attcttgtgc ttatttcaat acacctgaag
    10861 atacgttgga acaagcacaa atcaataaag tccatcatat cttagacaaa ctctttatca
    10921 aagaaggcga aacgttgctt gatatcggtt gtggctgggg aacattgatg ttcactgcta
    10981 ccaaagagta taatgtcaag gcaactggga tcacactaag tgaagaacaa tacgatttga
    11041 ttaccaaaag aataaaagaa gaacatttag aagataaatg ccgtgtatta ttaatggatt
    11101 accgcgaact aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg ttcgaacatg
    11161 taggttctga aaatcttgaa ggttatttca aagtggtaaa agatttattg aaaccaaaag
    11221 gaacagcttt gatccatgga atcagccgac aacaaggcgg tgcgacaaat gcttggatca
    11281 ataaatatat tttccctggc ggttatgtcc ctggtgttac tgaattagtt gataatatta
    11341 caaaaaacga tttgcaactg attgatttgg aaagcttacg tcgtgactac caattgacat
    11401 tagaacattg gacaaagaat tttcatcaag tccaagatca agtcacgaaa gaaatgggtg
    11461 attccttcta tcgaatgtgg gatctatatt tacaagcttg tgcagcttca tttgaatcaa
    11521 gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac atcccaatgc
    11581 atcgtgcata attgaggcaa aagtactgtt taaagactat ttaaaaagaa gctaagtttt
    11641 atgatgctcc agaagtgaac ttttttgtct aacttctagg attgtctcta aagcttagtt
    11701 tctttttctt tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa
    11761 aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca
    11821 atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa
    11881 tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca
    11941 caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct
    12001 agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc
    12061 caagataaat acccagatac tgcaattact agtttggaat tagatagtga ttggggatct
    12121 tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct
    12181 caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt
    12241 caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca
    12301 aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat
    12361 ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag
    12421 atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg
    12481 aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag
    12541 gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga
    12601 tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag
    12661 aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac
    12721 cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt
    12781 tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac
    12841 cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa
    12901 tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg
    12961 gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca
    13021 gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg
    13081 atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg
    13141 tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag
    13201 ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt
    13261 tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac
    13321 aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc
    13381 ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag
    13441 aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa
    13501 gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc
    13561 ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa
    13621 ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag
    13681 ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa
    13741 tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc
    13801 agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa
    13861 gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc
    13921 taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc
    13981 agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg
    14041 gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg
    14101 aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt
    14161 ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg
    14221 gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga
    14281 ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt
    14341 tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc
    14401 gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct
    14461 tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat
    14521 cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac
    14581 aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca
    14641 atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc
    14701 agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc
    14761 atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga
    14821 acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga
    14881 gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag
    14941 cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag
    15001 aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca
    15061 tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg
    15121 tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat
    15181 caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct
    15241 ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta
    15301 gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac
    15361 gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt
    15421 ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt
    15481 gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa
    15541 gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga
    15601 gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat
    15661 ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta
    15721 ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt
    15781 ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg
    15841 aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc
    15901 aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt
    15961 tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga
    16021 ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc
    16081 ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca
    16141 ttcccgcact agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct
    16201 taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc
    16261 gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt
    16321 ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg
    16381 cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag
    16441 tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc
    16501 tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac
    16561 agtatttaga aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt
    16621 ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt
    16681 tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac
    16741 catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga
    16801 agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt
    16861 tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc
    16921 tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata
    16981 tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac
    17041 ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa
    17101 agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga
    17161 agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta
    17221 caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga
    17281 acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc
    17341 gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga
    17401 gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta
    17461 gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa
    17521 ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat
    17581 ccaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg
    17641 cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa
    17701 aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac
    17761 ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca
    17821 gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt
    17881 caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac
    17941 tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc
    18001 cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat
    18061 gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac
    18121 caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat
    18181 catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa
    18241 ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt
    18301 cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat
    18361 gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat
    18421 gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc
    18481 agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa
    18541 agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca
    18601 aattgctgga acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg
    18661 tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag
    18721 tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag
    18781 caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac
    18841 catctattct gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga
    18901 aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga
    18961 cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc
    19021 agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag
    19081 cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct
    19141 tttgtcacga cctctcggct tatcctacga gtattattcg atacatttat atcaataatc
    19201 ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg
    19261 aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg
    19321 ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag
    19381 agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac
    19441 catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt
    19501 tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga
    19561 tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag
    19621 gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg
    19681 cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca
    19741 tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa
    19801 aagtaacttt gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag
    19861 ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt
    19921 tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat
    19981 gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt
    20041 gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg
    20101 gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa
    20161 cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca
    20221 atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca
    20281 agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc
    20341 tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca
    20401 aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat
    20461 ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc
    20521 tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata
    20581 gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct
    20641 agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata
    20701 gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt
    20761 gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac
    20821 atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca
    20881 agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta
    20941 ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa
    21001 ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata
    21061 ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag
    21121 aaaataaagc gaaca
//
LOCUS       DAHUWM010000026        20565 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHUWM010000026 DAHUWM010000000
VERSION     DAHUWM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.026"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            347..1036
                     /locus_tag="NK788_000687"
     CDS             347..1036
                     /locus_tag="NK788_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6769035.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1023..2231
                     /locus_tag="NK788_000688"
     CDS             1023..2231
                     /locus_tag="NK788_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6769036.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2525..3796
                     /gene="serS"
                     /locus_tag="NK788_000689"
     CDS             2525..3796
                     /gene="serS"
                     /locus_tag="NK788_000689"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCH6769037.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4345..5829)
                     /gene="guaB"
                     /locus_tag="NK788_000690"
     CDS             complement(4345..5829)
                     /gene="guaB"
                     /locus_tag="NK788_000690"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCH6769038.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6334..7029)
                     /locus_tag="NK788_000691"
     CDS             complement(6334..7029)
                     /locus_tag="NK788_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCH6769039.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7119..8219)
                     /gene="ychF"
                     /locus_tag="NK788_000692"
     CDS             complement(7119..8219)
                     /gene="ychF"
                     /locus_tag="NK788_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCH6769040.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8228..8419)
                     /locus_tag="NK788_000693"
     CDS             complement(8228..8419)
                     /locus_tag="NK788_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCH6769041.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8440..9330)
                     /locus_tag="NK788_000694"
     CDS             complement(8440..9330)
                     /locus_tag="NK788_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCH6769042.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9317..10084)
                     /locus_tag="NK788_000695"
     CDS             complement(9317..10084)
                     /locus_tag="NK788_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCH6769043.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10096..10809)
                     /gene="rsmG"
                     /locus_tag="NK788_000696"
     CDS             complement(10096..10809)
                     /gene="rsmG"
                     /locus_tag="NK788_000696"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCH6769044.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11041..12942)
                     /gene="mnmG"
                     /locus_tag="NK788_000697"
     CDS             complement(11041..12942)
                     /gene="mnmG"
                     /locus_tag="NK788_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCH6769045.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12959..14356)
                     /gene="mnmE"
                     /locus_tag="NK788_000698"
     CDS             complement(12959..14356)
                     /gene="mnmE"
                     /locus_tag="NK788_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCH6769046.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14655..15038
                     /locus_tag="NK788_000699"
     CDS             14655..15038
                     /locus_tag="NK788_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769047.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15206..15964)
                     /locus_tag="NK788_000700"
     CDS             complement(15206..15964)
                     /locus_tag="NK788_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCH6769048.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15982..16800)
                     /locus_tag="NK788_000701"
     CDS             complement(15982..16800)
                     /locus_tag="NK788_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCH6769049.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16797..17153)
                     /gene="rnpA"
                     /locus_tag="NK788_000702"
     CDS             complement(16797..17153)
                     /gene="rnpA"
                     /locus_tag="NK788_000702"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCH6769050.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17271..17405)
                     /gene="rpmH"
                     /locus_tag="NK788_000703"
     CDS             complement(17271..17405)
                     /gene="rpmH"
                     /locus_tag="NK788_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCH6769051.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17928..19262
                     /gene="dnaA"
                     /locus_tag="NK788_000704"
     CDS             17928..19262
                     /gene="dnaA"
                     /locus_tag="NK788_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCH6769052.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19460..>20565
                     /gene="dnaN"
                     /locus_tag="NK788_000705"
     CDS             19460..>20565
                     /gene="dnaN"
                     /locus_tag="NK788_000705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCH6769053.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQL"
BASE COUNT         5877 a         4202 c         3784 g         6702 t
ORIGIN      
        1 aacatgtcaa caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat
       61 aataatatat caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc
      121 tagcttacat gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga
      181 gacgtaaaat ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact
      241 agatgtctca ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt
      301 tttttgctag aataatagta acaatatgac tctggaggat ttatttatga aaattttgat
      361 tgctgacgat gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta
      421 tgaagcagta aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga
      481 tattgaatta cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa
      541 agagctacga aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat
      601 ggataagatc aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc
      661 gttagaagta atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc
      721 agatcagcct gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt
      781 taaaacaatc gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt
      841 gcttgctagt catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa
      901 acaagaaagt attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa
      961 gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa
     1021 aattgacgca agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa
     1081 aagaaatcag cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa
     1141 atgtttctat tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg
     1201 cgatttggga ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg
     1261 gccggaattt tattattccc tttttctttc tattagatat tggtgttttg tattggcgct
     1321 tgattcgaag atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg
     1381 caaacggaaa ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg
     1441 taacaagtat caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa
     1501 tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca
     1561 cgtccatcat tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc
     1621 ttttaaagta tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg
     1681 acttatttga atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg
     1741 acatgatcca gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa
     1801 atatccaaat acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa
     1861 aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa
     1921 cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata
     1981 acggagcaat gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag
     2041 aagaatctcg ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca
     2101 ttgtggcttt gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca
     2161 tcatgcacct tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg
     2221 acgaaagctg aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga
     2281 acccagtaca ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata
     2341 atttggaagt tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc
     2401 cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac
     2461 tgattcgtcc caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa
     2521 tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat
     2581 gactcggggc gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg
     2641 taagctacta gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat
     2701 cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt
     2761 cggtggcaac atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca
     2821 aattgcaact actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga
     2881 ggacaatgta gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc
     2941 acactgggaa attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc
     3001 cggtagccgt tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa
     3061 cttcatgctt gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat
     3121 cgtcaacagt aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt
     3181 ccaattggag aatactgatc taacattaat cccaacagct gaagtaccat taactaacta
     3241 ttataatgat gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc
     3301 tgcttttcgt tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca
     3361 ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt
     3421 ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt
     3481 gatggcctta tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt
     3541 atggatacct gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt
     3601 ccaagcacgc cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca
     3661 tactctgaat ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta
     3721 ccaaaatgaa gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct
     3781 gaaagttatt aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat
     3841 tttgtgaaaa tggctttcca catttttgca taaattggac ttaataccga gaaactggct
     3901 tttgaacacc atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa
     3961 aaaatggaaa aatagtccct catattttac cgtttgttga actaataccg agggtcagct
     4021 gattgaacac cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga
     4081 caaattcaaa aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc
     4141 aagggaacac tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa
     4201 gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga
     4261 gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact
     4321 tttgtcccac gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt
     4381 gatttgtaca tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg
     4441 cgcttcatca cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa
     4501 accgccgatc atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc
     4561 gattccttct ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt
     4621 tgaacctttt tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc
     4681 ttggaagatt tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat
     4741 cactgcatgt ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc
     4801 gtctgcaata atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt
     4861 gagctgaggt acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat
     4921 accaactttt acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc
     4981 aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg
     5041 gatcacacct gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc
     5101 taataaagca ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag
     5161 caatcgacca tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt
     5221 gatcgtgatc aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt
     5281 atgtttttgc aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa
     5341 gttgtctttt gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc
     5401 gcggtttgta attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc
     5461 actgatacga tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa
     5521 gaagaacgga tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc
     5581 ttgctggctg atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc
     5641 aatggccatc ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa
     5701 cttaatattt ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc
     5761 tgctggtatc aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca
     5821 gttggacatt tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt
     5881 tcatttaagt caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa
     5941 agcgtttaca tctgtgtata ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat
     6001 aagaacagcc aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca
     6061 gatgcaaact tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag
     6121 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac
     6181 cgagaaactg gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc
     6241 acagtccctt actattattt aactatggat aaacaaaatg aggttcttta ctacccctag
     6301 atatctagca aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc
     6361 aataatcccg tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat
     6421 gataactact actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga
     6481 aaacagtacg acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc
     6541 tggtttcttg cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat
     6601 ataaaagacc cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg
     6661 cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa
     6721 cagcaacgta ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg
     6781 cttattgatg attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct
     6841 tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc
     6901 ttcttcagac aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt
     6961 tcttttggtt aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc
     7021 tggttccatt atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag
     7081 acattctacc atattctaca gctagtttga tagccctttt acacattaaa gcggaataac
     7141 attacgtcgc cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct
     7201 tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg
     7261 ataaatccgc gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct
     7321 tttttgaatg tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct
     7381 aacaaatcat aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct
     7441 tctaaaaatt ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca
     7501 ctgaccacga tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa
     7561 tgattcgcgt ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt
     7621 tttgtcgtca ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc
     7681 gttcgggcag attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct
     7741 gccactgcat ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg
     7801 atagattcta agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga
     7861 tcaacacgtc cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa
     7921 atagcgtcta cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt
     7981 gcgcctttta cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc
     8041 ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg
     8101 ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca
     8161 ttaaacaaag ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata
     8221 aatttattca ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat
     8281 ctcgacgggt catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc
     8341 ccattcgaat gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga
     8401 tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat
     8461 attcaagata tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt
     8521 acctttccca tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc
     8581 ttcgctttct cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc
     8641 ttttccatca ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt
     8701 ttcagttact gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag
     8761 cgtacgggct tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg
     8821 taaagaaagc aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc
     8881 tacttctgct tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc
     8941 taacggattc agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc
     9001 ttcgtcaaag tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg
     9061 aaaacgtcgc tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat
     9121 gggttgaaag acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc
     9181 aaatgtttta cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac
     9241 ggtatcatct tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat
     9301 tcctcggcct aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt
     9361 agacttcagc tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg
     9421 cttctgataa acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt
     9481 tacgtacttc ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta
     9541 agactccctc gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca
     9601 gtaattggct taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat
     9661 cacttgctgt aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa
     9721 tataatcata ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca
     9781 tcattgacgt caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca
     9841 aattttcgtg ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat
     9901 catagatgtc tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt
     9961 gtgcatccat atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat
    10021 tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg
    10081 ccatctcttc tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta
    10141 tttctttggg gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt
    10201 cggcaaggta accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt
    10261 tgcttctgtc aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg
    10321 tttagccaag ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt
    10381 gacaaggtca aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg
    10441 atataaggaa gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt
    10501 attcagagaa tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct
    10561 aggaaaccct gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc
    10621 aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat
    10681 agctgtaagg ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg
    10741 attcatttga tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc
    10801 tggtatcata aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta
    10861 ctttaacgca aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa
    10921 gtaagaaaag ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag
    10981 agaactagct ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta
    11041 ttacgctggt tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact
    11101 taggtctgca gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat
    11161 tttctgcaat ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt
    11221 ttctggaatc tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt
    11281 gatgtagcct tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc
    11341 ttcattttct ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa
    11401 gtcgcttgct agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg
    11461 gacttcttct gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc
    11521 ttcttttttc ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc
    11581 catttctgtt aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg
    11641 tgaagtcagc aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac
    11701 accgatgtaa ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc
    11761 tgcattgata ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt
    11821 tgtttgtcca gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg
    11881 gtgtggctct actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc
    11941 taatccagca atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa
    12001 accttgaaca tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg
    12061 tggtttgtct gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac
    12121 cccttcaacg atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg
    12181 agtcgtctca cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc
    12241 tggtgtgctg aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga
    12301 atagtcgatc gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc
    12361 aaagccaagt tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc
    12421 tgatgaatat ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat
    12481 aacaactgct ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc
    12541 atcttcgacg atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc
    12601 gatcgtatgt ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg
    12661 gacagctggg ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt
    12721 cttacccatt tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc
    12781 gcctacagaa ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa
    12841 tgtttttgat cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc
    12901 aacaacgatc acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt
    12961 attttcctaa acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc
    13021 cgacaacttc tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag
    13081 gcattcctga atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca
    13141 acgcgatatg gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga
    13201 agaataaatc agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa
    13261 ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg
    13321 gcaaatctgt tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt
    13381 gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt
    13441 ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc
    13501 gaattccagc tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga
    13561 ttacatctcg agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat
    13621 ggtttaacaa acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt
    13681 cccgcagaac cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca
    13741 tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat
    13801 aatcaatatt tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga
    13861 tcaaagcaga cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt
    13921 ttgcccgaat caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga
    13981 atgctcgttt tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct
    14041 gcaaaatttg attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc
    14101 gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc
    14161 tcttgggatc aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt
    14221 tgtttccaga ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa
    14281 cgatactgat tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta
    14341 aagtaatttc agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac
    14401 tccacccttt atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa
    14461 attctggtca tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg
    14521 aagttgagcc tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc
    14581 gttctttggt ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa
    14641 aaacggtaca ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg
    14701 tgagcccgtt cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg
    14761 ttttatctac agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg
    14821 aatgcttaga agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa
    14881 cgaagaggaa attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc
    14941 attttgagta tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg
    15001 gaacagtgat ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg
    15061 atcaaaactg aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc
    15121 gtatactctg ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac
    15181 gtgatttatt caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat
    15241 aacgataagg ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag
    15301 catggatttg tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc
    15361 gttttacttt ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac
    15421 ggtaattgcc tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga
    15481 cttgtgccag atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt
    15541 gcttttgtgt ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa
    15601 gagctggagc atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag
    15661 ctgcttcatc acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga
    15721 ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg
    15781 tttcagaaac tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca
    15841 ttcctaggaa accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca
    15901 aacccaaatc agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg
    15961 gcattagcat tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag
    16021 agcacgttct ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa
    16081 agggttgttg atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa
    16141 tgaaagaccg ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt
    16201 catgatcttc attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc
    16261 aaaagtaaag attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt
    16321 caaccataag aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc
    16381 catcaagatt ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt
    16441 ttctgcatat aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga
    16501 actatattgt tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct
    16561 ctttgtttgg aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat
    16621 gatccctatc cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac
    16681 aatataacga tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga
    16741 taagacgatg accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac
    16801 ttactctttt cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag
    16861 cgaacctcat cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac
    16921 tccggatcaa tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca
    16981 ttgcggttta ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc
    17041 tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta
    17101 tggaacacgg cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt
    17161 ctttcctttt ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc
    17221 atcatttccc taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa
    17281 agaacttttc ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc
    17341 atacgtttac ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt
    17401 ttcatatatt ccacctccaa aatgttctta tctattttat aaacacagac atactttgat
    17461 agtataacga caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt
    17521 atccacaaga ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt
    17581 atccacagat aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc
    17641 acaatcaggc gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta
    17701 tccacagacg caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg
    17761 tgtggaaaac tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg
    17821 ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc
    17881 taaattagta ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg
    17941 atgctttatg gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata
    18001 atacatggat cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg
    18061 ttcctagcaa gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag
    18121 tcggttatat gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag
    18181 agcaagaaga agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa
    18241 aagccatgct gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga
    18301 tggctcatgc agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct
    18361 tcttttatgg aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa
    18421 tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg
    18481 atttcatcaa ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg
    18541 ttgatttact gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag
    18601 agtttttcca tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg
    18661 atcggttgcc aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg
    18721 ggctgtctgt tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa
    18781 aagcggcggc tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa
    18841 ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga
    18901 tgaatagtga agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag
    18961 gaaaaggaca tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc
    19021 acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac
    19081 aaatcgctat gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg
    19141 aattcggtgg caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga
    19201 tggattctag tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt
    19261 aatttgtcga ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag
    19321 ttatacacac tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc
    19381 acagaatcaa catgccctat tacttttatt attcttttta actaataata aataaacaaa
    19441 cattcaaagg agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt
    19501 gcaaactgtt caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa
    19561 aatcacactg acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga
    19621 aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat
    19681 tgttttacaa gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac
    19741 tttagaagtt ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt
    19801 aaatggatta gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat
    19861 gaaattacct gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca
    19921 acatgagagt cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt
    19981 agctgtagct actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc
    20041 tgatcatttt gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac
    20101 aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga
    20161 aacgatgtat ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat
    20221 tccttcaagt tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga
    20281 acgggcttct ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc
    20341 agatgcggtt gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag
    20401 ttatacagca agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc
    20461 agcgttgcgt gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc
    20521 atttacattg gaaccaacgg aagatggcgt tcaatttatt cagct
//
LOCUS       DAHUWM010000027        20539 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHUWM010000027 DAHUWM010000000
VERSION     DAHUWM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.027"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(271..1296)
                     /locus_tag="NK788_000706"
     CDS             complement(271..1296)
                     /locus_tag="NK788_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6769054.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1571..3256
                     /locus_tag="NK788_000707"
     CDS             1571..3256
                     /locus_tag="NK788_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCH6769055.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3270..4553
                     /locus_tag="NK788_000708"
     CDS             3270..4553
                     /locus_tag="NK788_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCH6769056.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4623..5492
                     /locus_tag="NK788_000709"
     CDS             4623..5492
                     /locus_tag="NK788_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769057.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5489..6325
                     /locus_tag="NK788_000710"
     CDS             5489..6325
                     /locus_tag="NK788_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769058.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6351..7418
                     /locus_tag="NK788_000711"
     CDS             6351..7418
                     /locus_tag="NK788_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCH6769059.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7415..8242
                     /locus_tag="NK788_000712"
     CDS             7415..8242
                     /locus_tag="NK788_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCH6769060.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8287..9312
                     /locus_tag="NK788_000713"
     CDS             8287..9312
                     /locus_tag="NK788_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6769061.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9309..11492
                     /locus_tag="NK788_000714"
     CDS             9309..11492
                     /locus_tag="NK788_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCH6769062.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11492..12622
                     /locus_tag="NK788_000715"
     CDS             11492..12622
                     /locus_tag="NK788_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCH6769063.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12619..13296
                     /gene="pgmB"
                     /locus_tag="NK788_000716"
     CDS             12619..13296
                     /gene="pgmB"
                     /locus_tag="NK788_000716"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCH6769064.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13380..13907
                     /gene="msrA"
                     /locus_tag="NK788_000717"
     CDS             13380..13907
                     /gene="msrA"
                     /locus_tag="NK788_000717"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCH6769065.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14108..14794)
                     /locus_tag="NK788_000718"
     CDS             complement(14108..14794)
                     /locus_tag="NK788_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCH6769066.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14874..16412
                     /locus_tag="NK788_000719"
     CDS             14874..16412
                     /locus_tag="NK788_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCH6769067.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16532..16999
                     /locus_tag="NK788_000720"
     CDS             16532..16999
                     /locus_tag="NK788_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCH6769068.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17518..17961
                     /gene="rplM"
                     /locus_tag="NK788_000721"
     CDS             17518..17961
                     /gene="rplM"
                     /locus_tag="NK788_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCH6769069.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17977..18369
                     /gene="rpsI"
                     /locus_tag="NK788_000722"
     CDS             17977..18369
                     /gene="rpsI"
                     /locus_tag="NK788_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCH6769070.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18457..19683)
                     /locus_tag="NK788_000723"
     CDS             complement(18457..19683)
                     /locus_tag="NK788_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6769071.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(19755..19913)
                     /locus_tag="NK788_000724"
     CDS             complement(19755..19913)
                     /locus_tag="NK788_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769072.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
BASE COUNT         6622 a         3703 c         4275 g         5939 t
ORIGIN      
        1 cccaaataac atggattgaa ataagggtaa atgtgtttaa aaaaacgaac tataaaaact
       61 tatacacaac gtggttaaaa atagtttttt cttccctgtg gaaaaaatag tttaacacaa
      121 acattatagt tatccacagc tatctaaaac agatggaaaa taagaaaaat tcgctaaaat
      181 accgggaggg ggtaacgagg gatgagtgat tgaaaaaaga acagagtaac tgacttatcc
      241 tcagtgtttc tgttctttta taattctgtt ctacatttct tgttgttgga tcgaatctgt
      301 gagagtaatc tcggttgaaa gaatgattct tttttcttca gggtattggt ggtggattag
      361 ttggaataaa agttctgtgg ctttgattcc taactgattt gtcggaatgt ctatccgagt
      421 caactgtggc gaaaataggg aggacaataa gggatcactg aatcctgcga tcgaaagatc
      481 atcaggtatc tgctttccag attgttctgc cactttgtaa atactcaatg attttaatgc
      541 atcggtagaa aaaatcgcag ttggtctttc tcgtagcttc attaaatcat tcattgctgc
      601 catgctatct tctaagcgaa agccgccgtc tataatccat tcattacgga tatctaggtg
      661 gtgatcaaat aagcattgac ggtaaccatt gagacgatca ttggatacat aaacttctgt
      721 cggagcatgg agacagccga tatgacggtg tccattcttg atcagtgttt ccaccaaatc
      781 gtaactatcc tggaaattat ccgtgtccac tgagtagatg ttcgtgtagt cgtttccgac
      841 ttttccgatt accacaatag gaatattata ttgatccaat tcaaaaagaa gtgtttcatc
      901 catcgtagag cttaaaataa taattcctcg gatcattttt tcttggatct ttttcgcgca
      961 tttttctagc tcttcttctg gtgtttttgc cgtttgtaga atcaagtcga agttattttg
     1021 ttcagaaacg gcagaaatgg attgaataac atctgaaaaa aaaggattgc cataggtcgt
     1081 atgagaagta cgatgagata atgctagaat cgcgtcaaaa ccatttcctg acagtcctct
     1141 tgcaattttg ctcggttcat aattcaattc tttgattgct tgaagtacac ttgcttttgc
     1201 tttatcagag atatttggct ggttatttaa tactctggag acagttgatt ttgagacatt
     1261 agcttttttc gcaatgtcat aaatagtagg tttcaatttt atatgcacct cctttttggg
     1321 agcgttttag aataattcca tagtacaata gacattcttt tttgtctatg attttgaaaa
     1381 tttaggaata gcttgcatga tcatgagaga ccatattttt ttaagaagct attaattatt
     1441 gatacataag ctttcttatt tgtatttttg tgtatatcaa aattatttaa aaaaatgcta
     1501 ttgacagtaa gcgttttcca taatatgatc aaatcgttgg gaacgctccc aaaaaagaaa
     1561 ggggaatccg atgtatacaa aacttttaca agcgatttat cgcgactcag atttgtctga
     1621 gaaaaccgcc tctatgattg aacaaaaagt cagtgtttat cagcagaaga aaggccaggc
     1681 aggcaaactg cattgggacg aagaagatat tttcttgatt acttatgggg atcaattttt
     1741 tgaagagcag caaccaactt tgcgtacatt taagaaattt tatcaaaagt atttggcaga
     1801 tacatttaga gttgtacatt tcttgccatt tttcccatat acatctgatg atggtttctc
     1861 cgttgtaaat tatcaggcaa tcaacgaccg tttgggcaca tgggaagata tagaagaaat
     1921 gagaagcgaa acacggttaa tgttcgactt tgtctgcaat catatgtctg ccgaaagtac
     1981 ttggtttcag gaatatttaa acttgaatga tacgtacaaa gattttttta ttgatttgcc
     2041 actggataca gatacaagca gcgtgactag accaaggacc agcccgttac ttacaacgtt
     2101 cattgacaaa aacgggaaag aaaaacacgt atggacgact tttagcgaag atcaagtaga
     2161 cttgaatttt aaaaatccag aagttttatt gaaaatgatc gatattcttt tgttttactt
     2221 agaaaaaggc gcttcattta ttcgcttgga cgctgtggga tttttatgga aagaactagg
     2281 aacttcttgt atacacctgc cagaaacgca taaaatcatc caattattcc gtgcaatcgc
     2341 tgatgaagca gctccaggaa cggtattgat cactgagaca aatgtgccac acaaagataa
     2401 tatcagttat tttgggaatg gcgagaatga agcacagatg gtctatcagt tttcgctacc
     2461 acctttgacc cttcaagcga ttcgttcggg taatacaagt aagttgaaag aatggttaaa
     2521 aaccatcgat ttcgcatcga aaaaaacaac tttttttaat tttttagcct cccatgacgg
     2581 tatcggattg aatccggtac gaggtattct gccggaaaaa acgatccttg aattagtgga
     2641 acagctgaag aaagagggtg cccgagtaaa ctacaagaaa aacccagatg gaacgaccag
     2701 tccatatgag ataaacgcta cgtatctaga tgcgttaagt tctccaacag atacgacgga
     2761 atggagaagc aaacgctttt tagctgctca ttcatttttg gtaacgttac caggcgtgcc
     2821 ggcaatttat attcaaagtg tgctgggaag tcgaaatgat tacaaaggcg tagaaaaaac
     2881 aggagagaat cgaaaaatca atcggcaaaa atttttgtta gcagatattg agaaagagtt
     2941 gcagacagat ccattaagaa cagcagtata tggtgaattg acccagatgc tatctattcg
     3001 aaaaaaagaa aaagccttcc atccgaatag tttgatttac ttactggact cagcagattc
     3061 ttttatcagt tttattcgcg aagcgaagga agagaaagtc ttagttatcc ataatttttc
     3121 caatcaatca gcaggttatc ttttaccaaa aggagattac ctagacctgt tttctggaca
     3181 aattatccat tgcagtcaag aacgaaagtt gatattccag ccgtatgaat atatgtggct
     3241 gaaaataata aaataagagg tggaaaaaaa tgaaaaaaat tattgttact acctgtctgc
     3301 tagctactgg atttcttctt actgcttgtg gtgaaagtca aagctctaac gaatcacaac
     3361 agacaacgat tcaattcatg cactcttctg tcgaacagga acgattaagt gtgatcaatg
     3421 aactggttgc tgattttgaa aaagaaaatc cagacatcaa aatcaagcaa gtgccggtag
     3481 aagaatcagc gttcaataca aaaatcgtta cgttagctcg ttccggtaaa ttaccagaag
     3541 taatggaagt cagtcaggat tttgctaaag tgatggataa agatgaactg attgatcaga
     3601 cggcagtcca aaacgttatc gaggaagctg gagaagataa ttattatgac ggagccaaaa
     3661 accttgttcg ttcagaagat ggaaaaagct atattgctgc accaattagc ggctgggttc
     3721 aaggtatttg gtatgacaaa gaaaaattat cagaagctgg tttttcagaa ccagaaaatt
     3781 gggatgatat tttaaaaatt gctcaacatt tcacagataa agagaataaa caatatggaa
     3841 ttgctatgcc tactgctgaa ggaacaatga gtgagcaagc gttctctcaa tttgctttgt
     3901 caaatggagc aaatgttttg gatgatgaag gcgaggtaac gatcgatacg gaaaagatga
     3961 gagaagcatt gtcgttttat caaaacttat cccaatacac gatgccaggc tctaatgatg
     4021 tgacagaaat caaagatgct ttcatgaatg gaacagcacc aatggcaatc tattcaactt
     4081 acattttgcc ttctgtttat gaagcgggga actcagaaaa tatcggtttt gctatcccaa
     4141 cacaaaaaga tcaagctgtc tatggcacag tatctggatt gacgatttct tctggtctag
     4201 acgagaaaca aaaagaggct gctgaagcat tcacagcttt cttatctcaa ccgaaaaaca
     4261 tggaaaagtg ggtcttgatg tctcctggtg gtgctcaacc agtgaataaa caagtagtcg
     4321 aactggatgg ttacaaagaa aatgaagtca tcaagtcttt tggcgagctg ccatctgaga
     4381 ttgctagtgc ttttgatgaa gtacaagtct ttggactagt cggggaaaag aatttcacga
     4441 aaatgggaga tatcacaagc tcaggtgtga tcggaaaagc agtgaaccaa gtaactgtcg
     4501 gaaacgaaga tgtcgatcaa gctttagctg acgcacaaaa aaatacgaaa taacaagaag
     4561 ttctgccaat ggtgttcgaa aaaagtagat agctattggc agattatcag ggggaggttt
     4621 caatgaatcg tccaaggaaa cattctgact taaaattagc catgggactt ttagcaccaa
     4681 gcatgctttt attagctgtt ttagtgctga ttccaatgct ttccaatatt cagatcagtt
     4741 ttttcgatat gccgctgaat cctaactcag acgccgtttt tatcggatta agcaattatc
     4801 ttgaaatttt aagtgatagt gaattttatc gttctttagg tttaacagtt gcttacacat
     4861 tcttagttgt agcaggcagt acagctttag gattagccgt agctattttg cttaaccgca
     4921 agttcagatt taggaaaacg gtgcgttcac tgattttatt aagttatgtt acgccatcta
     4981 tttctttgat ttttgcttgg aaatatatgt tcaataatag ttatggaatc gtcaatgatc
     5041 tgttagtaga taggttgcag cttttcaacg aagcaccgct gtggttcgac cagccagttg
     5101 ctagcttctt tatggtcgtt ttgtttgcta tctggagata ctatccgtat gcgtttattt
     5161 catttttggc tattttgcaa acagtcgaca aaacgcttta tgaagcagct gaaatggacg
     5221 gtgcaaatgc ttggcagaag ttcaagatcg tgacactgcc ggcaatcaaa ccagtactag
     5281 caactgtgat tgttttacga tctatctgga tgttctatat gtttacagat gtctatctac
     5341 tgacgaataa agtcaatatc ttaggcgttt atttgtatca gacagccttt gcgttcaatg
     5401 atcttggaaa agcagcagct atatcgattc tattgttcat cattattgca gcggtcgtct
     5461 tgctctcacg aaagaaggtg gacttgaaat gaaaaataaa aagagcagga aaatcttatt
     5521 ttacgcgacg attttgttgc tattagctgt caatttgttt ccatttttga tcatgctgat
     5581 gacatcattc aaggattcac aagaagcaat tgcaacgaca caaactattt ttccgaaaga
     5641 atggactttt gaacattatc aggatatttt caatgctgag atttttccat tcatcatgta
     5701 tttcaaaaat agtttagtga tctccttgat agcctccgta actgcagtag tgattgggat
     5761 cagcggggca tacgctttgt caaaactacg cttcaaagct cgctcgaaaa tcaatgctag
     5821 cttttacacc gtttatatgt tttccggtat tttgatcgtt gttccattat ttaaaatcat
     5881 ttccggtcta ggtttgtatg ataccaatac agcactgatt atcacgatgg tcgttcaaac
     5941 acttcctact gcgatattca tgctaaaaag ttattttgac acgattccga ctgatattga
     6001 agaagcagca atgatcgatg gtcttagccg gatccagatc attattcgga tcattatacc
     6061 attggctatt tctggaatca tttctgtctt cgtttattgc tttatggttt cgtggaatga
     6121 ttatttattt gcttcgattt tcctgtcgac ctccgaaaaa tttactttac caatcggttt
     6181 gaacacatta ttcagtacac cagactacat ttggggacga atgatggccg cttcattggt
     6241 gaccgcttta ccagtcatca ttatgtatgc gttatctgaa cgatttatta aaggtgattt
     6301 agtcgaaggc ggcgtaaaag gataaaaaga aaagaaagag gaaagaaaag atgaaaaaat
     6361 tagttgcatt agaaccaaga atagccggat tcaaagatta cgaagaccgt gagatcgaaa
     6421 agaacgaagt acgtatccga gtaattttcg ctactccaaa accaggaaca gaaattgtcg
     6481 actttagagg attaagtcca tttatcgatg aagaattttc agaagaatgg cgcctattct
     6541 tgccaagaga agaaggaagc caacgcggtg tggtatttgg cgaatttcaa ttaggaaata
     6601 tgattgtcgg tacgatcgaa gaaacgggca gtttggtgac agagtatcaa aaaggagagt
     6661 tagtctgcac ttatggaccc attcgtgaaa cgctgattac aaacggtgtt gataattata
     6721 aactacgtaa acttgatcgg ttggatcaat ggaaaaatgc agtatgttac gatcctgctc
     6781 aattcgcttt aggtggtatc cgggaagcac atgttcgagc aggtgatgct gtagcaatct
     6841 tcggtttagg cgctatcggg caaattgcga ttcaattagc taaaaaagca ggtgctacta
     6901 ccatcgttgg gatcgatccc atagcgcagc gtagagaatt agcagaaaaa cacggtgccg
     6961 attacacgct agatcctatg aaagcagatg tcggttttcg tctaaaggaa ttgacagata
     7021 aacgtggagt agattcagtt attgaaacga gtggcagtgc tgccgcatta caagcttctt
     7081 taatagggat cggctatggc gggatcattt cttatgttgc ctttgctaaa ccttttccag
     7141 ctgggtttaa tcttggtcgc gaagcacatt tcaataatgc aaaaatcgtc tttgctcgag
     7201 catctagtga accaaaccca gattacccaa gatggaaccg taaacgaatc gaggacacat
     7261 gttgggaatt attgatgaac ggttacttgg attgtgaaga aatcattgat ccagttgtgc
     7321 catttgcaga aagcgcagaa gcttacatgc gttatgtaga ccagcaccca gaagaaagta
     7381 tcaaaatggg cgttcaagtc cgaggaggag aacaatgaaa ttcggtacgc aagatcaagc
     7441 atttttccca agagacattt tagaaaaatt tagatttatc aaagactgtg gatttgaaga
     7501 atacgaaatc gacggtaagt tgttaataga aaatatcgac acagtcaaac aggcaatcaa
     7561 agaaacaggg atcccagtag tgacagcttg taacggctac gatgggtgga tcggtgactt
     7621 tatcgaagaa cggcgtctta atggtgtacg tcaaattgct gagattttgc gtgcactaag
     7681 cgaggttgga ggcaaaggaa tcgttatccc agcagcttgg ggaatgttca cttatcgatt
     7741 accgccgatg gtttcacccc gaagcaaaga aggagactat aaagcagtca gcgaatcact
     7801 tcgttattta gataaagtag ccgaagaaac aggtacggtt atttatttgg agcctttgaa
     7861 ccgataccaa gaccatatga tcaatttgtt agcggatgcg cgtcattaca ttgactctaa
     7921 tcaattgaaa catgtgcaga tcatcgctga tttttatcat atgaatattg aagaagatga
     7981 tatttcaaaa gcattgacta cgcaaaaaga gtatgtgggt catatccatt tagcagataa
     8041 tcatcgctac caaccaggat ctggcgcaat tgattttact cgccattttc aaacgctact
     8101 tcaaaatgag tatgaaggtt atatggtata tgaatgtcgt ttgcgaggag aaaatgaagt
     8161 gaaaatttat caggaatcat tgcaatactt gaaaaactgt ctggaaacag cgcgtcattt
     8221 aaatgattta gagaaagaat agagaaagaa taacaataag taaataagca aaggagagag
     8281 aaataaatga agaaagtagc aatcataggc gctggacaag tagcagaaaa agtacatgct
     8341 gcttattatc aaaaaagatc agatgtctta gaattggccg ctgtggttga tccagtcagc
     8401 cagcgagcag aagacttttg ccgacgtaca ggattttctc atgcgtatac atcagttaca
     8461 caaatgctga atgagatttc tcccgatatc gtgagtgtct gtacacccaa tcgctttcac
     8521 ttcgaacatg tattggctgc cctcgaagct aatgcttcag tgatgtgcga gaaaccacca
     8581 gctatgacag ctgatcaagc aagaaagatg gcagagacag ccgataaaaa cgggcagatt
     8641 ttggcttatg gattccagca tcgtttttct gacgaagcaa caatgatcaa gaatcatctt
     8701 gatgttctcg gggatattta ttacgtagaa gcgaatgcgc ttcgtcggtc tggtgttcca
     8761 ggctggggaa cgtttatcga caaagaactt caaggaggcg ggcctttgat cgattatggc
     8821 attcacatgc ttgatacagc gatgtatatt actggatttc ctaaagtaaa aagcgttcgg
     8881 gcgtacagct tccaaaaaat tggtacgaaa aaaaattcag ggaaattcgg gaaatgggat
     8941 ccaagcaaat tcacagtaga agatgcaatt tttggcacac tggaattaga aaacggcaag
     9001 atcatccaac tgaatacttc gtttgctttg aatatcagag aagacaaact gaatgtggct
     9061 ttttgtggcg atcaggcagg tgctaatctg tatccaggcg aactattcga agatgtagat
     9121 ggaaagctgg tttccttaca aaagaccacg ccagttaatg acccacatga actaagtatg
     9181 gctcattttg tgaatcgggc gctcggtgac cagactcaga tgattgcaga tggctggcaa
     9241 ggatatgtca tccagaatct ggtggaaaat ttatatcgtt cggcagaaac aggagaagtt
     9301 attaccttat gaaagtagaa gaaaaacagt ttgatttaga gtatatcaat aagtatgcga
     9361 gtcagatgac cattggaaat ggattttttg gtatcagagg aagccaggag gaagactatc
     9421 ggaatcaagt aaggggaatg tttgcagctg gtgtatataa ccgaccttta ggagcagaat
     9481 cagccgagtt agtgaatctc ccagatgtca tcagatggga aataaaaatc gacggtgaac
     9541 atttttctct tgagacgatg aaagttcttg catacgagcg ggcactagat ttagaaatgg
     9601 gagagcttaa acgctatgcg ctcattgaaa ctgctcaagg aaaaaaagtg gaagtcatta
     9661 cttgtcgtgt tgctgatcaa aataatctcc atcaggcagc cctgaaagtg gtcatcaagc
     9721 cgttgaccga agactgccaa gtagatgtga aaacgggtat cgatggacag caaaccaatt
     9781 ttggtacaca gcagttggtt gaaaaagaac tgcgagtatt tggtgacgat ttgatcagtg
     9841 catcctacca gacaacagaa agtaaaattg atattgggat tgcagctaaa ttcaataaag
     9901 caggtgtatt ccaagcaaaa aatcgccgga tcaccgcttc cttttcagaa aagatcagaa
     9961 aaaatacgtc ctttacgctg gagaagatca tcgtgctctc tacctctctt cagcaaacag
    10021 acccgtttaa gaaaagtatt gcccaagccc agaaaagcgt tagttataca gaaattttag
    10081 agcagtcgat gtcttattgg acagattttt ggcatcagca tcggataacg atccaaggcg
    10141 attcttcttt cgatcagtta gcgatggact tcgctgctta ccatttacag attatgactc
    10201 cccgcaatga tccaagtgtt agtgtagggg cgaagggact aactggcgaa ggttataaag
    10261 ggcatgtttt ctgggatacg gaaattttta tcctgccttt ctttttatat cagtttcctg
    10321 aagtagcaaa gaatttgctg ctgtaccgtt atgatcgatt agaaggcgca cggcaaaaag
    10381 caagagatta tcactatcaa ggtgcacttt tcccatggga aagcgcacac actggtcagg
    10441 aagagacacc caaatttgct gcattgaata tcaaaaccgg aacaagacaa gaagttgctt
    10501 cagctgccgc agaacatcat atcagtgcag accttgctta tgccgttcaa gagtatgtcc
    10561 aagcaacaga ggatcaatcg tttttagaaa caaagggaaa aacgctcatt catgaaacag
    10621 cagctttttg gctgagcaga gctgtaaaag tcgatgatca tttagagata cacagagtca
    10681 ttggtccaga tgaatacaca gaatatatcg acaataatgc atatacgaac tatctggcgc
    10741 attataatgt ggccttagct gttaaatatc aagctggcga tccgcttttt ttgaaagaat
    10801 gtcaaaattt tttagataag ttatatttgc cagtgcctaa caaagaacag ctgattccgc
    10861 aagatgacac gtatctcagt aagccagtta ttgatttgac cacttacaaa gaacgtcctg
    10921 gcagccagtc tattttactt gattatgccc gtagcgaagt aaatgaactc caagtcagca
    10981 agcaagcaga tcttgtcatg ctgttatact tattacctga gtatttttct caaccaatcg
    11041 tttctaagaa tttgcattac tatgaaaaca aaacaatcca cgactcatcg ttgagtaaag
    11101 cgattcacgc catagaggct tgccgtatcc aagaaatgga atgggcgtat catttattcc
    11161 aagaagcgtg cctgattgat ttaggaccag atccgcattc atcagatgac ggaattcatg
    11221 ctgcttcatt aggagcacta tggctagcag ttatatttgg tttttcaggt attacagtaa
    11281 cgaatggaac tctttctatt tctccaagac ttccttcagc atgggaaaaa atttcttttc
    11341 cttttatttg gaaaaatcac aggttgctat ttgttttgac aaaaaaacaa atagtaataa
    11401 aaaaagagac gaaagaaaca ttacctattt ttgtcaatgg agaccaatac cagctggaaa
    11461 aagaactgat cttggaacgg aggggaaaat aatgcgtatt ctgattgcgc cagattcttt
    11521 caaagaaagt ctgacagcct tagaggcagc acaagccatt caatcaggtt ttcaagaagt
    11581 ttttgttcag gctgaatatg atctgctacc tataggcgat ggaggagaag gaacggtttc
    11641 atcattagca gacagtttaa ggttgaagcg gcaaacagca gtcgttacta atgcatttgg
    11701 caaaaacaaa gaaatccagt acgcttcaga cggtcaaact gcgatttttg aaatggcaga
    11761 aatcgtcgga ttgaaagatg tcccactaaa ccaacaacag ccacttaggt tatccacgcg
    11821 aggagtagga gaactgatac gacaattgtt gcaacaaggt gttcgacaca tcatcatcgg
    11881 cgttggcgga agtagtacca acgatggcgg catcggcatg gcagaaggtc taggttatgc
    11941 tttttacgat caagataatc aaaaagtaga agcactggga gaaaatctag gtaaaattgc
    12001 tacagttgat aaaacgaacg tgcctattga gctgattaaa gccaaaatca ctgtcattac
    12061 tgatgttact aatcttttat gcggaaaaga aggcgcttct tttgttttcg gaccacaaaa
    12121 aggcttgaaa gaaaatgaat tagcagtggc tgaccaagcc atgcatgatt tttatatgag
    12181 atttgcccct caagtaatcg aggcatcggg cagtagtgct ggtggtggaa tgggtgcagg
    12241 actgttagca tttgcttcag ggaaaatagt ctctggcatc gactacgtat tggatttact
    12301 ggagttcgat aaacgggtac agcaagcaga cctcgtaatc gtaggcgaag gacgtctaga
    12361 tggacaaagt ctaaaaggaa aagcgccagt tgggattgct agaagagtac cagagggcgt
    12421 tcctgtgata gctatttgcg gaagtgttgg taaaggaagc gaaaaagcag cagatgttgg
    12481 catttcggcc gtttttccga tccttcctgc agctgtgcct ttacaagaag ctttacagca
    12541 agccactgat aatcttcatc gcacagctaa aaacatcgca tctttacttc aagtcgttca
    12601 ttttcaagga ggtaatcgat gaaaatcaaa gcagcactat tcgatttaga tggcgttctt
    12661 gtagacacag ctcgttatca ttacgaagcg tggctggttt tagctaacca gctgtccatt
    12721 ccattcactg agaaagaaaa cgagcaactg aaaggaatat ccagaaccga gtcactagag
    12781 cgattgctgt catttggaaa aatggaacag aaattctcag aaaaagagaa atcagccttt
    12841 gctgagcaaa aaaacaatct atatcttcaa gcgattcaaa aaatggatga aacatccgtt
    12901 ttgcctggtg caatagctgt tcttgagtac ttgaaaaaaa caaacatcaa gatcggattg
    12961 ggatcagcaa gcaaaaatgc acgcctcatt ttagaaaaaa cgaatttgac aagttatttt
    13021 gatgtcctta ttgacggcac gcaagtatca aaagcaaaac ctgatccaga agtttttctc
    13081 aaaggagcac agcaattgaa tgtaccgcca aatgcatgct tggtcattga agattcagaa
    13141 gctggttgtc aagcagcttt ggctggtaac atgcatgtat tgggaatagg tgaaaacata
    13201 aatcttcctt ctgctgaata tgtgattcct gatttaaccg tttttgatca agtacgttcg
    13261 ttttggcatt tatcagaagc cgtatcacgt atataatagt ttccgttttt tctgtcacaa
    13321 gcaaaacgtt atcctccttt tggaaatcaa ggtatgatga gaaaaaaggg ggattttata
    13381 tgacaaaaaa agcagttttc gcaggcggat gcttttggtg tatggtcaaa ccatttgaag
    13441 agcagccagg gatcatttct gtgacatccg gttatactgg cgggcatgta ccaaatccaa
    13501 cttatgaaca agtaacaaca ggcacaacag ggcatacaga agcagtagag atcgaatatg
    13561 atccagaagt tatttcttat gatcagttgg tggaaatcta ttggcagcaa accgatccga
    13621 ctgacgcatt aggacagttc gctgatcgcg gtgattctta tcgtccagtg atctactata
    13681 atgatgaaga agaacgaaat attgcagagc gttcaaaaca agcattgcaa gacagcgggc
    13741 gattcgataa gccaatcgtg acaaaaatcg aaccaagaag cgaattttat cctgcagaag
    13801 attaccatca ggattattac aagaaaaaca agctccatta caatctttat cgagaaggtt
    13861 ctggacgtgc aggtttcctt cgtcgacatt ggggcgtcaa gaaataagaa gccgaaaaag
    13921 ctacttatgg aacaccgtat atcagaggtt gtaaaaccag cattttgagc taatactgag
    13981 ggtcagctga ttgaacacca tttagtggag aggttgtgaa cctgtcgcct agctccaagt
    14041 cacaaggatt tggtaagaga acactatata tcagaggttg tgacaagtcg tttgtcacaa
    14101 ccttttttca atacctaaga tgaaggacaa tcagcaacgc actgctcaaa aaaggaatga
    14161 aaggcagctt ttccaacttt tttttatgac agataaagta aacagaaaaa acggatattc
    14221 caaacagact agcaagaaac aagatctgaa gcaactcttc tagagacaag ccaccactcc
    14281 aagaaagcat caacagagta tcgccagctc cgagatagga ttgaccatag cgaaggcaca
    14341 caccgcctac gataaacgtt aacagcacac tttgccattg gaagacgccg gcctgcaacc
    14401 aaaaaatcca taaaattgcc caagaacaga atagcgtgtg agaatggacc atgaagtaaa
    14461 ataaatccat caacgaaaat agcaaagccg aaagcatcca tatgaacgtc gaaaaatttc
    14521 ttgcggaaga aaaagtaaag aaccaggcaa aaagaccacc acataaggct tctgccaaca
    14581 aatagtagaa gggcaaccga cacctgcaat agcggcagcg aaatcgttgg acgatgatcg
    14641 agagaatcgg aatcaattca taccaagaaa gcgaacgaca gcatttgaca caatgagatc
    14701 taggataaat aatagagtga cctaacggaa tccgctgggc ggttaaacat aaaaatgatc
    14761 cgatacagca accaatgata aaataaagta acacgccatc tccccctttt caaaaccaag
    14821 atacgcaagc ttaaactaaa tttttgtaca tacataaaat ttgaggtgaa aaaatgaacg
    14881 tttcaatcgg attagatatc ggaaccacac aaaccaaagc agttgctttc acagatgaag
    14941 cccaagaagc agcatccgct tattttcgct accctttgat ccaagaaaca caagggatgg
    15001 cagaacaaga tcaggaattg atctttcaag gtgtctgcac cgttataaaa gaagtaatta
    15061 aacagttgcc ttcagacaca atcattcaat ttgtctcctt ctcctctgcg atgcatggcc
    15121 tgatattgtt ggatgaagag aaaacaccgt tatctcgaat gatcacttgg gcagataacc
    15181 gagcagcgga cgaagcagaa aaactgaaat ataccgtgct tggcaaagaa ttttatcggc
    15241 agactgggac gccgattcat ccaatgacac cattgaacaa aatcaagtgg ctgcaaaaca
    15301 gccaacctga attattcaaa aaaactgctt attttgtcgg tatcaaagac tatatctttt
    15361 atagattgtt tggcgaactg atcagcgact acggaacagc ttcaggaact ggatattttg
    15421 atatccatga attccaatgg gcagaagaag tgcttgacta cttgaacatt cgaatagagc
    15481 aattgccgca agtctttcct tccacctatc aagtaacagg tctaccatca aaaacagcag
    15541 cagaactagg tttgccaaat gatatatcat ttgttttggg aggggcagac gggccgcttt
    15601 ctaatctagg gttaggtgct tttggtgata cgatcgccgc tttgaccgta ggaacgagcg
    15661 gagcacttcg ttttattact actcagcctc agttccatcc agaaatggag acgttttgtt
    15721 atgttctgga tcagaaacat tgggtagtgg gaggagcgac tagcaatggt gctggcatct
    15781 ttgattgggc agggcataca tttttacagg aagtcgttca atctgctaaa gaaaaaggag
    15841 aaaatccata tgatgcactg ctttcggcta tcgcttttgt gccagcgggt gccaatggtc
    15901 tgatttttca tccctattta cttggtgagc gagcaccgct ttgggacgca gatgcgtttg
    15961 gcagttttat cggtttacag cggcaacata cagaaaaaga aatgatgaag gcgattctag
    16021 aagggatctg tctcaacctt tatcggattt taaaaggagt ttgccaggaa aatcagctca
    16081 cagagatccg tgctacaggc gggtttgctc aatctccgat ttttcgtcag atgatggctg
    16141 atgtactggg ttacccgctt gttttcccta ctgtcacaga agcatccgca ttaggagctg
    16201 tgattttagg ctggagaagc atcggaaaga tccaaagtat cagtgaagca caggaattga
    16261 tagaattgaa agagcaagtt ggagtacaag aaactgatca taaaaaatat cagaaaatct
    16321 atccattatt tgtttcgaca caaaagcatt tagcacagac ttatcagcaa tttgctcaac
    16381 tgcgagaaga attagctgat tctgaaaaat gattggcttt acggataaaa aagcgaactg
    16441 aaaatcgttc tcaggtaaat gattttgttg tacttttcat tgaaagtcta ctattatttt
    16501 aagtgtaaga aaatgttagg aggcaagaaa gatgaaattc caacaaacca aagaagtatt
    16561 aaatcaatta gttgcagatc taagccaaat gtctgttgtt gttcaccaaa cacattggta
    16621 catgagagga ccaggtttcc ttacattgca cccaatgatg gatgagttca tggatgattt
    16681 gaacgctcag ttagatgaag tatccgaacg cttgatcaca ttagacggtt caccttattc
    16741 tacattgcgt gaatttgcag ataacacaaa aatccctgat gaaattggcc gctgggatcg
    16801 tactattcca gaacgtttgg aaacacttgt tgcaggctat cgttatctag cagatcttta
    16861 ccaaaaagga atcgaagttt ctggagaaga aggcgacgat ccaacacaag atatctttat
    16921 cgaattcaaa acagccacag aaaaacgtat ctggatgatc caagctcatt tgggcaaagc
    16981 gccagagatc gacgcgtaaa aactgttgtt gaacttgacc gaaaaagagg ttgtgaaatc
    17041 agcgttttga cctgagtatt agagaaacaa acgtcaaaat agttcctcat attttgccgt
    17101 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttaccaagag gttgtaagcc
    17161 tgccgttcag ctccgagtca caagaaacaa ttttaaaaaa cagcttctta tgttttatgg
    17221 atattgtgac ttgtgaccga ggagttggca ggagaacacc gtttattcaa aataaggggt
    17281 tgtgacaaga cttttgtcac agcctctttt tgtttttcat tttgacagga aacgaccctt
    17341 ttatataaaa gaaagaaaag aagtaaagtt tcatataaat ttttcaatca agcagaaaaa
    17401 cgttgacatt gactagatta gcgggtaata tgttaaatgg tattgtttgc cccacaaaga
    17461 gccccggaaa ctcgaagtgt atgtcaaaca tcagaactta aattggagga aaataacgtg
    17521 cgtacaacat atatggccaa accaggcgaa gtagaacgta aatggtatgt agtagacgca
    17581 actgatgttc cattgggacg tctttcaaca gttgttgcat ctgtactacg tggtaaaaat
    17641 aaaccaactt tcacacctca tttggatact ggcgatttcg tcatcgtcat caatgcagac
    17701 aaagtgaaat taacaggtaa aaaagcgact gacaaaattt attaccgtca cagcaactac
    17761 ccaggaggat tgaaatcaat cactgctggc gaattgcgtg ctaaaaattc tcgtcgtttg
    17821 atcgaaactt ctgtaaaagg tatgcttcct aaaaacactt taggccgcaa acaatttaca
    17881 aaattgaacg tatacggtgg agcagaacat ccacatgcag cacaacaacc agaagtttta
    17941 gacatcacaa acttaatcta ataggaggga atttcattgg cacaagttca atatatcggc
    18001 acaggccgtc gtaaaaacgc agttgctcgc gtacgtctag taccaggaac tggcaaaatt
    18061 actgtaaata aaaaagatgt tgaagaatac attccacatg ctgacttgcg tgaagtaatc
    18121 aatcaacctt tcgctgttac tgaaacaaaa ggcgcatatg acgtatttgt taacgtaaac
    18181 ggcggaggat acgctggaca atcaggagct atccgtcatg gtatcgctcg tgcattgcta
    18241 gaagttgatc ctgacttccg tttagctctt aaacgtgctg gcttacttac tcgtgacgca
    18301 cgtatggtcg aacgtaaaaa accaggtctt aagaaagcac gtaaagcttc acaattctca
    18361 aaacgttaat atcctttata tatcaacgtt tctggcactt ttcaattatt tggaaagtgc
    18421 ttcttttgtt tttaagaaca catttaagaa cacatatcaa tcacacttat tcaatcctct
    18481 ctgaaaagct tcaaaaacag ccttatcaac tatatctggt gtgttcacat atatctttgt
    18541 tgtagaaata tctgagtgtg ttaaagcatt tgaaatttga ttcatatctg ctccaccttg
    18601 tctagctata gttgcataag tatgacggag tttgtgtgga gataaatgaa ctaagtcagg
    18661 atgccttctt ttaattgaat tgattcgata gtttaggtaa tcggcatgaa caggacaatt
    18721 tacttctcca gatggtttag tataagtaaa gagatactga tctaacgttt gcttaatttt
    18781 taatttcaac aattgatcag cttgtaccga tttccattca cttagcaatg tcattacaac
    18841 ttctggtact tgaatgacgg tatcttttcg accttttgta aacttttttc tttgatattt
    18901 atctaaagta tgtttaagac ggaccgtttg tttttcaaaa tcaatatatt tccattgcaa
    18961 agcgtaagtc tcactttttc tatcaccaag atacagggta atcataaaaa gtgtgtaatc
    19021 atgaaaagta agtaaatgat tattaaaatc atcatttact gcttctatcc aattagtaag
    19081 ttcttttgat gatagtgctt gcttaccttc ttgtattctt ttttcttcta aagcattttt
    19141 tttaggtgca gtgatacttt gaataatttt agtagttcga ttaaattcga tataatttag
    19201 tatttctgca atatcgaaca tggaacgaac atatcctttt atgattttta tatttgcata
    19261 ttctttcgat tttttggtta attcatctga tattatctca gtattgatat ttagataatt
    19321 cattgcgtat tcaccaaaca taggtaaaat atgcaaccga aatatatctt ttgtattact
    19381 aatggttata tcactaggag gcctgtttga tctaattgtt tgacctaatt catatctagg
    19441 aagccattta gattcgtaaa attttttaaa cgttattttt ccttttaatt ctaatgagtt
    19501 tgcattctcc tctctcaaaa ctttttctat tttcttcttc atatcatttt cggctttaat
    19561 cgccaattgc ctatttggaa atgttttttg atacttttct gatgcaattc ctgttatttc
    19621 tttaaacctt tttggatatc tcagattagt tttatatttc ccatttgaaa gctttgtagg
    19681 caaaatcctc actccttaaa aaaagtaagg cagtatattt cactaatttt agtatatgcg
    19741 gcgagaagaa aatttcaagt tattttaaat gtattcataa agtctttaat atcttctacg
    19801 tcaaagcgaa ctacaccacc aactcttatt actgggaaat tatatatacg aatccactta
    19861 tctagagtat tattatcgat tcctagaaat gcacaggttt gttttttatt caggtagact
    19921 ctggattcta taaaatcatc tcctaaaata gtagttatga aatttttgaa ttgctttggg
    19981 taagcacata ggaatttcac ctctccccag tttacggcga gccttacgga agtatcatta
    20041 tttgctgcta ttgtcatggc attttgtttt cccagaacaa aaagaaatct ctaagtattt
    20101 gtcgctaatc aggtcatggc gtacttgagt ttctagctcg aatagcaggg atcgttattc
    20161 aaaaattgta tttaattgtc aatgatcaaa aatagttttt ttcctattca ctattaataa
    20221 cgataaacat gtataaaaag acacactacg aaaaaagtta aatggagttt actgattttc
    20281 atatttgtat atggaacaga catagtcttg aataattttt ataaatgttt tgatgcttat
    20341 caacttattc aacccgctat ccacttcaag tataattttt ccatacgcca ataaagtagt
    20401 tttaggagca tgttctcatt tgagaacgct ccttttttat atattttgaa agctacttac
    20461 tatacgcgta gtttcaaata gttttgtcat tctaatcttc agcaacagaa aaaaatctaa
    20521 actataaaat ttagggttg
//
LOCUS       DAHUWM010000028        20436 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHUWM010000028 DAHUWM010000000
VERSION     DAHUWM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.028"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            38..340
                     /locus_tag="NK788_000725"
     CDS             38..340
                     /locus_tag="NK788_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769073.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            337..498
                     /locus_tag="NK788_000726"
     CDS             337..498
                     /locus_tag="NK788_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6769074.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            495..800
                     /locus_tag="NK788_000727"
     CDS             495..800
                     /locus_tag="NK788_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769075.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            800..1156
                     /locus_tag="NK788_000728"
     CDS             800..1156
                     /locus_tag="NK788_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCH6769076.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1143..1361
                     /locus_tag="NK788_000729"
     CDS             1143..1361
                     /locus_tag="NK788_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769077.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            1358..1777
                     /locus_tag="NK788_000730"
     CDS             1358..1777
                     /locus_tag="NK788_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCH6769078.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            1774..2331
                     /locus_tag="NK788_000731"
     CDS             1774..2331
                     /locus_tag="NK788_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCH6769079.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            2328..2624
                     /locus_tag="NK788_000732"
     CDS             2328..2624
                     /locus_tag="NK788_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769080.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            2701..3114
                     /locus_tag="NK788_000733"
     CDS             2701..3114
                     /locus_tag="NK788_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCH6769081.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            3460..3834
                     /locus_tag="NK788_000734"
     CDS             3460..3834
                     /locus_tag="NK788_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769082.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            3871..4038
                     /locus_tag="NK788_000735"
     CDS             3871..4038
                     /locus_tag="NK788_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769083.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            4064..4408
                     /locus_tag="NK788_000736"
     CDS             4064..4408
                     /locus_tag="NK788_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCH6769084.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4413..4694
                     /locus_tag="NK788_000737"
     CDS             4413..4694
                     /locus_tag="NK788_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769085.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            4797..5111
                     /locus_tag="NK788_000738"
     CDS             4797..5111
                     /locus_tag="NK788_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCH6769086.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5089..6783
                     /locus_tag="NK788_000739"
     CDS             5089..6783
                     /locus_tag="NK788_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCH6769087.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            6803..7981
                     /locus_tag="NK788_000740"
     CDS             6803..7981
                     /locus_tag="NK788_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCH6769088.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            7944..8630
                     /locus_tag="NK788_000741"
     CDS             7944..8630
                     /locus_tag="NK788_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCH6769089.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8630..9790
                     /locus_tag="NK788_000742"
     CDS             8630..9790
                     /locus_tag="NK788_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCH6769090.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            9800..10675
                     /locus_tag="NK788_000743"
     CDS             9800..10675
                     /locus_tag="NK788_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769091.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10672..10983
                     /locus_tag="NK788_000744"
     CDS             10672..10983
                     /locus_tag="NK788_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769092.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            10973..11326
                     /locus_tag="NK788_000745"
     CDS             10973..11326
                     /locus_tag="NK788_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCH6769093.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11316..11717
                     /locus_tag="NK788_000746"
     CDS             11316..11717
                     /locus_tag="NK788_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769094.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11710..12114
                     /locus_tag="NK788_000747"
     CDS             11710..12114
                     /locus_tag="NK788_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769095.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12126..12734
                     /locus_tag="NK788_000748"
     CDS             12126..12734
                     /locus_tag="NK788_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6769096.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            12754..13116
                     /locus_tag="NK788_000749"
     CDS             12754..13116
                     /locus_tag="NK788_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769097.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13119..13301
                     /locus_tag="NK788_000750"
     CDS             13119..13301
                     /locus_tag="NK788_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769098.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13318..16737
                     /locus_tag="NK788_000751"
     CDS             13318..16737
                     /locus_tag="NK788_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCH6769099.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            16788..17525
                     /locus_tag="NK788_000752"
     CDS             16788..17525
                     /locus_tag="NK788_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6769100.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17535..19826
                     /locus_tag="NK788_000753"
     CDS             17535..19826
                     /locus_tag="NK788_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6769101.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            19850..>20436
                     /locus_tag="NK788_000754"
     CDS             19850..>20436
                     /locus_tag="NK788_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769102.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7322 a         2988 c         4392 g         5734 t
ORIGIN      
        1 gatcaagtac tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca
       61 gcgaattatc gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga
      121 agtgatgagg cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa
      181 aatcgcaatt tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag
      241 tctcacaggc ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc
      301 taagaagaga acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa
      361 atgcgtattc gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg
      421 gtcaatgaaa cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc
      481 catcgattgg aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa
      541 ccaagtcaga atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta
      601 aatcaatgtg gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg
      661 atgaccgaat tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca
      721 aaaacattat ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta
      781 ggtatgtgga ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa
      841 aatcatgatg aagattcaga aagataaaaa atcaaaagaa cgagctgaat tagttaagtt
      901 agaaatggct gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa
      961 cattgaattt tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg
     1021 gcaaacaaat tggagcaaga aacttcatca agatcgtttc aaatttgtag agaagtataa
     1081 agaaatatta gaagagtatt tgaggaggac agcaaatgat aaaaaaactc gttcaattca
     1141 gcatggattt atatgatatc gaatcaggag caacactatc tgtggaatcg gaccatctaa
     1201 tcataaattt tggtggaaag cgccagatta ttttgtgggt agttgatgat gtactgtttc
     1261 cagaaattgt tcatgatttc gaagaatcaa aagcggttga gtttgaaata gtgaaaaaag
     1321 taatggaatt gattgaaaaa tacgaggagg acagcgaatg ataccgaagt ttagagcgtg
     1381 ggataaacga aagaacgtaa tgagagatgt agccgtcttg cattttacta aaaacggcaa
     1441 aacaaacttt attgaatatt ggataaatcc taccgaattg aaatcatatc acgtgcgaaa
     1501 catcgacctc atgcaatcca caggaatgaa agataagaat ggtgtggata tatttgaagg
     1561 ggatatagta ttagtcagcg tgcgaaatgg cttcgattac ttagataata aagtctgtat
     1621 tgtcaaaaat tcaatagatt attccggatt agtttgtgcc actgttgatg aagacttaga
     1681 gtatcaaatt tttaacacag agctgtttga agaatacacg tacgaagtca ccggaaatat
     1741 atacgagaat agcgagttat tggaggaaca gcgatgaata aacaggaagc aatcaaaaag
     1801 ttagaaagta ttaaagcgat aggaaatgat gcaatagctg cttgctataa cgagagtata
     1861 aattcaggta ttacgttaat gaaaaaaata gacgaaccgc agaaaccagt tattccacaa
     1921 ctcgtggccg gttggcttga gaaatctacg gaccctttta caaaagctga aaaaatagcg
     1981 tatttaatca aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat
     2041 ggcatagtga cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat
     2101 gaaacactat tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata
     2161 aagaatgccg atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat
     2221 tatagttttg aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat
     2281 gctgcagcat tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg aaactaaaag
     2341 acggatttta cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga
     2401 accctaaaac acgcaagaaa tcaaaattca aagtcggtga catggtccgc tgtgaagcag
     2461 aagagttcgt ttatccgttc agaggatacg ttaaaaagat actgtcaaac tcagcaatca
     2521 ttcgtattga aaatacaatg aagtgtgatc gttcgactgc aaaacacaag cattatttag
     2581 ctgtagcgag attagttgac atggaagtaa tcaagagcaa ataaaaaaag ccggatcgct
     2641 ccgactgatt caataaatcc aacacattta ttatatcaca taaaggagcg gtttgacttg
     2701 atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg
     2761 aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg
     2821 attattacgg atatgccgaa ggcaccaaag cacggcaata aggcagaaga cgcgatcatt
     2881 cagatgatgg atatagaagc ggagagagat gcgattctag cggctttgat ggctcttagt
     2941 ctaattagtc gccagatact ctactacagc ttctgtgtgc cagatagctt ctcaaactac
     3001 aggattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa gtcggaagct
     3061 ctaatagagt ttgcagaagc atataaacac ggaagaataa ttgcttataa ataatttggc
     3121 ggttttttgg cggaatgatg gcggttttta gctatttacc agtgatatta tggtagtgtc
     3181 gaaagattag tgataggtct aagacaaaat aaaatgtaag ggaggaaatc tccctcatcg
     3241 tttaattaag cttcgataga cagcagcaaa taaactaaag gatgtggggg tcagctccta
     3301 cagttagttc atgtgttgct gtctattaat tgcaactctt tcggttttat tgagtattta
     3361 ctcaacttaa aaaaactgtt gtttgtagat gtaagctgtt cctatttgta catagtaaaa
     3421 aatgatataa taaaaatatt acgaaagggg tgagagtgca tggaagacaa aatgtattta
     3481 agacaaaaag ttttcacaaa aaaagatatt gaaaatcaaa ttaatggatt tatacagaaa
     3541 ttagaaaatg caaattctta tttgataaat aaggaactaa ataaagctta tgaccagtgg
     3601 aaagaagtat atgatgagtt gaaattgatt caaaatgaaa caaaattagt acgaacggag
     3661 aaaaagaatg aaaatagttt cttttttgat ggatatgcta tattgatgtt ggaaactgta
     3721 gcaaaacaaa atataaaagc tcccaaaaaa gaactatctg acaatattga taacgcatta
     3781 gccgagttaa ggtattacgt tatgcaaata aaagatgtta ggatcactcg ttgagtggtc
     3841 tttttatttt gcacaaagga ggtaacaaca atgtatagac cacaatactt agaacagaag
     3901 tatgaagtaa tcactgtgca aaatggtaac ggagagatag tacgaaagta tagaagacca
     3961 ataaagagcg atacatataa acgaaaggaa agcaatgaag ttattccatt gtatggcaaa
     4021 agaatagcta agcattaaat aagattgtga aaggagacgg gacatgaacg aggaattcta
     4081 tagatggcta ttacagttga taagagaaga tcgtttggtt aagttctatc agtctcctaa
     4141 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     4201 aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag
     4261 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     4321 agttcatggc agatatctta cagcattgga taaacaagag aagaagatag aaagctttgc
     4381 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgataat ggcagagagt
     4441 atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga
     4501 tatacgttcg ctatgtaggt attagggatc tgttatcaga taactgttgt agtaaataca
     4561 aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga ataaaaaatg
     4621 tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa gctttcattc
     4681 cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg
     4741 agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt
     4801 ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc
     4861 aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca
     4921 gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg
     4981 agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat
     5041 tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc
     5101 ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta
     5161 tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat
     5221 atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca
     5281 ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa
     5341 aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa
     5401 actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat
     5461 tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta
     5521 tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa
     5581 gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca
     5641 aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat
     5701 tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat
     5761 gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa
     5821 atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt
     5881 ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa
     5941 gcttgtccaa ctattaatta caatgcaaat ttaaaacgga aaatgtttca agaatactct
     6001 caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct
     6061 atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa
     6121 gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat
     6181 tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact
     6241 tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct
     6301 atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta
     6361 ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac
     6421 cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga
     6481 agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa
     6541 cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag
     6601 catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat
     6661 ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct
     6721 gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc
     6781 taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa
     6841 aaaagcaacg gttcctttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg
     6901 aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt
     6961 caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc
     7021 tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt
     7081 ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga
     7141 tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa
     7201 caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga
     7261 tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag
     7321 tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt
     7381 ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa
     7441 ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt
     7501 agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga
     7561 catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat
     7621 gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga
     7681 acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa
     7741 ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc
     7801 tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag aacgcgttgg
     7861 aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca
     7921 agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg
     7981 aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag
     8041 ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg
     8101 aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac
     8161 ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt
     8221 caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc
     8281 accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac
     8341 tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg
     8401 aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt
     8461 atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag
     8521 aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg
     8581 catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa
     8641 aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa
     8701 cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt
     8761 agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt
     8821 attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg
     8881 ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc
     8941 tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt
     9001 gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt
     9061 agataatggc tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt
     9121 atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt
     9181 agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat
     9241 gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa
     9301 agcagaacct attacagctt tagatgctgt aactatgggc cgtttgatgg cgcgactatc
     9361 aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt
     9421 ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact
     9481 aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac
     9541 ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg
     9601 atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta
     9661 tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga
     9721 tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac
     9781 acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa
     9841 atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt
     9901 gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga
     9961 agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata
    10021 tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat
    10081 cgattcggat gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga
    10141 tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga
    10201 aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt
    10261 ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgtc ctaaagcgaa
    10321 ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa
    10381 aacaaatata caaatctgga cagccgagat gtgggcacgg ctttggaaca tgatgtattt
    10441 caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga
    10501 aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt
    10561 atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa
    10621 caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct
    10681 gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac
    10741 gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa
    10801 gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga
    10861 tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta
    10921 ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa
    10981 taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac
    11041 caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa
    11101 atttagaaat ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa
    11161 attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca
    11221 atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt
    11281 ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa
    11341 aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc
    11401 tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca
    11461 ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc
    11521 tatatctaac tttctattca gaaaatagag aagatttaac aggagactca ctagatatta
    11581 tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac
    11641 tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga
    11701 gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt
    11761 gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca
    11821 aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat
    11881 ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat
    11941 ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg
    12001 ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca
    12061 cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa atagggggaa
    12121 caacaatggc taaaacaaaa actgtagtaa caacgttcga taacgtaagt atcaaacgaa
    12181 ttgcttttaa ttttaagaac gcaggaaatg caatcgcaac gaattgtaat ggacaattag
    12241 atggcgaaac agaaatgcaa acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa
    12301 aatctaaacc aatcaatatg acggtaacaa ttactgcaca tgtaccgatg gaagtttatc
    12361 gacgtttcaa tgggttgaaa caagatggac gtattaaacc aggcatttac tcttacggtc
    12421 ctgattccgt aggcgaagat ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa
    12481 atagaaagtt agttggtatg ttagcatgca cttcgaatac aggattaaca ttctctattg
    12541 aaaatggtgc ggatgaagta gctgcgttag aactagaaac aaaagttatg caagatgaat
    12601 ttggtaaatt ttatcatgaa gcaattgttg cagaacttga agaagactta acagatcaat
    12661 ggatgacgaa tctatctgct gatgtgatta aaaaaacttc agttgtgaca actacggcca
    12721 ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg aagattactc aaaaattgaa
    12781 ctaaacgatg gaacaatttt gaatttagaa cctaaactga atatcaagaa attattgatg
    12841 atcaatagag attttaacac agacgagttt gcaaaaatga ctgtgggaaa aggatccatg
    12901 gatatttctg ttattcaagg tgcaaaggct gtgtatattg cttaccgcca agcgaacatg
    12961 actgattata tttcattcga tgaatttatc gataaatggg attttgatat ggctactgcc
    13021 agctatattt atcaattgat gatgttcaaa caagcacgcg atgcttatca aaaagaattt
    13081 gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat gccaaagctc ttagttgaaa
    13141 cgtgggtcga tgtctattcg atgttgaccg acgttttttc tatgccttca gatttggttt
    13201 taagcgatat ctgtttagat gacattttgc aaatggctta caacaagagt gcttatgaag
    13261 gatggaaaaa ctatgcaata aaccaatctc agaaaaacta aagaaaggag gtaaaaaatg
    13321 gctaaaaaga gaacagaagc agaagtaact ttcatagcta acgatgacgg attgaaatct
    13381 acgttaaaag aaatcagtgc tgaattaact aaaaatagag cagaattaaa actagaacaa
    13441 gctcaattac aacagactgg ttctgaatca gacaagttag gaagtaaatt atcttcttta
    13501 gaaaagcagt atgaattaca aagccaaaaa gttgaagtaa ctagccaacg tttagccaat
    13561 gccaaaaaat attatggaga aaattccacc gaagttcaga aacttgagag agaactgatt
    13621 aaccaacaaa cagcgcaaca acgtttgtca aacgaaattg ataaaacgag taatacacta
    13681 gctcaagcaa aaggcgaaat acagacgtac gagtctacaa tgcaacagtt ggatagtgaa
    13741 caaaaaaatg ttcaagctag tgcttctctg attgaatccg aatacaaaaa atggcaagta
    13801 actgctggtc aatcagcttc tgaatccgag aaattagcga aagcccaaga atatgtttct
    13861 caacaatctg aaaatgcaga gaaaacgata gatatcctga gacgacagtt agaagctaca
    13921 cagtctgagt ttggcgctac atccacagaa gcaatgcaga tggaggcgaa gcttaatgat
    13981 gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa atgtagatac aactaacttg
    14041 gacgatatcg gaagcaaaat agatatgaat aatttaatgg aagcttctga cgttttaagc
    14101 gacattggcg ataagcttac agaattaggg aaacaagcag tggactctgc taacagtgta
    14161 ggtagttccc agagtaaaat acaagctaat tttggtttga ctaaacaaga ggctgaagaa
    14221 ttaacgaatg tagccagaga catttattat aaaggttttg gagaatcgtt agatcagtcc
    14281 acagatgcat tgattttggt aaagcgtaat ttaggcgatt taaataatca agatttacaa
    14341 aatattacgg aacaagctat ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg
    14401 ttacgtggtg taaatggctt aatggtcaac ttcggattga gcgctcaaga tgcaatggat
    14461 ttaatggttt cgggtactca aaacggttta gataaaacgc acgaattagg cgacaatatg
    14521 gcagaatata gccaattatg gagtcaaatg ggatattcag ctgatgaaac gttcggaatg
    14581 cttcaaaatg gtttagatgc gggtgcttat aaccttgata aagtcaatga cttagttaag
    14641 gaaatgggaa tatcgttaac agatggtcga tttgagcaaa acatggatat gtttagtgaa
    14701 agtactagaa aagcttttga agagtggaaa aatggtggag gaacacaaaa agacgttatt
    14761 aattccatga ttcaagattt tagcaatatg gatggtcaat acgaccaatt aaataaagct
    14821 tctacaattt ggtctgcact tggcgaagat aacgcgatga aagttgtcca atctttgact
    14881 gatgttaacc atacatttga tgatgttagt ggatctgcac aaaaaatgaa tgaagattct
    14941 actactccgt tacaagaatt aaacggaaaa atagctgaat taaaggattc attagctcct
    15001 ataggcaaca caatcataga tgcactcgaa ccagtaattg attttctagg aaagatggct
    15061 gatgcgttta ataatcttcc acaaccagta caggattatg ccgtagcgat tggcggattg
    15121 actgctgcat ttactttatt aatgccaata atagttggct tcatggctct aggtggtcct
    15181 actacattaa taataggagc agttattact gctattgctg gagttatagc aattataaaa
    15241 aactggggcg caattactga ctggtttaag ggaatatgga gtaaattcac tgattggttg
    15301 ggtggtactt gggaaagcat aaaagaaggt gcctcatcag tttgggatgg agtcaaagaa
    15361 acctggtctg gatttgtaga ttgggttcaa gaaatttggc aaggagtttc tgattggttt
    15421 ggagagttat ggagcggatt agttgaagga gcttccaaca tctggcaagg agtccaagag
    15481 acttggcaag cattcgttga ttgggtttca aatatttgga acggagtcaa agaagtatgg
    15541 tcgattattt gggcagacat tgtaggaatt gttcaaatac catggacatt aataacgtca
    15601 ttgattcaag ctggtattaa tattatcgtg ggtatctttg atgtagctgg acagttatta
    15661 ggcgcagctt ggcaagctgt ttggacacct atttctgatt tccttaaaaa tacttgggat
    15721 actatgacac aatggataag catcgcttgg aatggaattg taactacatt ccatactata
    15781 tttgatccag tagtggcatg gtggaatggt atatggacat ctattagtac tacggcttca
    15841 aatatttgga actcaattag tgcaacagct tctagtattt ggaatagtat caagaataca
    15901 atcactagct tggtacaagc agctgctaca gtaattcaaa atatttggtc aactgtatct
    15961 agttggttag gtggaatttg gaattcaatc agctctacag catcaaatat ctggagtagc
    16021 gtgactagta gtataagcaa tgctataaac gcagctaaaa gtgccattca aagtgtttgg
    16081 aatagtatat cttcgtggat cagcggaatt tggaacggta tcaaaaacac tgctttgaat
    16141 ctttggaatg gaattacaag cactattagc tctaaagtaa acgatggaaa aaatgcaatt
    16201 tcaagcggtt ggtccaatct aacaggtatt gtttccgaca tattcaataa tgttaaaagc
    16261 acgatagcta atatatggga aggtatcaaa aagactgtta gcgctccgat tgattggatt
    16321 agagataaaa tcagtagtgt ctttgataat ttgaatattt ctataccaca tattccgtta
    16381 ccacatttta aattgagcgg agaattcaat ccattgaagg ggaaaatccc aacattgggt
    16441 gttgattggt atgcgaaagg tagtgtgttt aattctccaa atattatcgg tgtaggtgaa
    16501 tcaggacctg aagcagtttt acctttgaaa agatctgtgc tgcaagaaat tggtgatcgt
    16561 atcttgagta gcacctcagt ttcatctagg acacaagcgg ttcaacctgt gaacaattac
    16621 gaattcaatt tcacaattga tggtaacgca gatgaagtca ctatgaagca aacaactcaa
    16681 caaatcattg atagcattac aaaagttcaa aatgataatg cttcggcatg gcgttaaaca
    16741 ggagagtatt tctcctgttt ttttagtatt aaaaaggatg tgaaaaaatg actgattgta
    16801 tacattctat aatcgatgga tttcctgatt atttgcataa attggcttta gctgaaagac
    16861 caaccatacc ttctccaaaa agacagagag ttgaaacttc tgttttagga aggttaggtg
    16921 gcttagtgca agattactcg tttgaagaca tgtcgtttac attgcactat aactatttag
    16981 aggatgtgga agaccatcaa gcgttcaagc aatcgtttta tatcatgcgt cattggttaa
    17041 actatgcaaa gaaattagaa ttctctgacg atcccaacgt ctattacgtt atccagacta
    17101 tcgatattgg ggatgcagaa aacgatattg ttgaatgggg agagttcgat gtaaatatta
    17161 ctgcgaaacc attcgcaaga gttcaagaag atgtacctat aaccgtagat aaaccacagt
    17221 catttaactt gctgaataat agtttagaag aaagttttcc aaagattatc atcactcctt
    17281 cagctacttc atgccagttc atcttaaatg attatgtgtt tagtttcgaa ggcttagtag
    17341 caggaactga tgtagtcatt gatagtgatt tgatgctttg ctacgaagag caatcggacg
    17401 gagatatttt agatcggtcc aacaaaatga agaccatgca atatccgaca ttgcaagtgg
    17461 atatcaatca ttttaattgc actggtttga gcaaaataca aatttatcgt aacgggttaa
    17521 ggtaggtgaa atagatgatc gataatttaa taactattta cgataaaaat gacgcgaata
    17581 atttagctga acatttatat gatacgcaag gtttaggcgc tttgtcagac tggttaacag
    17641 ctactgttag caataaacta aacggagccg agatatttca gggtacttat ccaataagcg
    17701 gaactaatgc agatttgatt gtagaaggac gtattattca gtgttatgta gatgaaaatc
    17761 gagcaaaaca gcgtctacgg atttattatg caaaaacttc tgtaataggc aatacgatag
    17821 aagtaaaagc tgaacctatt ttcaatgata taagaaaatc ggtgttgaat aaatatgaca
    17881 gcggaacaga aaaaatcact gctactcagg catggcaaaa cgcaaaagct ttagcgaaac
    17941 cagctatccc ttcgcagttt tctttctcgt cattagtaga tacgcttgct aatgtgaaga
    18001 tagaaaaagc gaatttttta gaattctttg gtggaaaaga gggatctatt ctagatcgat
    18061 ttcatgggga atttcttaaa gataataaca cattacatca tgaaaaaagg ctaggtacgg
    18121 atcataaaat caaagcgatt tatactaaaa atttaactgg tcttgactta gagatagatg
    18181 ctcaaagcgt tttagttgga gtttatccat tcattagcag ttcttcagaa ggagaagacg
    18241 agatcactct accagaagaa gtcattttca cggattacgt ggatgattat cctgctggat
    18301 atgtttcttt tgttgatttt aaagacaaag cgactgatgt agccacatta agggaagctg
    18361 ctaaagactg gttgaaaaca aacatagata aacaaaaacc acaagtgagt ggttcgattg
    18421 aattagtacc attgaggcat caaagaggct atgaaaaatt tgttgatcta gaaaaagttt
    18481 cgatgggtga cggagtagat gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa
    18541 ttgtggaata tacgtttaat gttctaacca attcatacga taaattagtt gtaggaaacg
    18601 tcaaaacaaa cttcttagaa aacacagaga ataatgtaag caatttgatt aatgatgcca
    18661 ttgatcaatt gaaaaatggt ggcgaaatca gtgatttaat caatgatatt gtagatcatc
    18721 aaactgatat gattactggt caaaatggtg gttatgtttt attagatcct aaagaagcgc
    18781 ctagtcgtat tttgattatg gacacaccag ataagaatac cgcaaggaat gttttacaaa
    18841 tcaacaatgc tggtattggt ttttctaaaa ctggcattaa tggaacatat gaaaccgcat
    18901 ggacattaga tggcggattc aatgcctcgt ttattacggc tggtgaaata gtaggaatta
    18961 ctattagagg tactacatta attagtgatg gcactgatta tagaacaagt attgctaatg
    19021 gcaaaatgac ttggtactca aaaaaagtta acaaagatat tatggagcta gaagcacgtg
    19081 attatgtaag tgctgatgcc ggtattgtat catacaccat gaaaactggt ggtggtttca
    19141 tgattagaaa tccacagggt aacttggttt ttagtacgtg ggataatggt aataacagac
    19201 cgtttctatc ttttggtgcg cccaatttca ggtatagcaa tgctagttat gtaacttctg
    19261 gcgacggtag ttctttaagc attaatggta gtgcgggtaa ctcatgggaa tttaaggtag
    19321 ctggtaggac tatgaaattt actagtgatg gtatgctaac gttgccaggt tgtttttttg
    19381 gttcatggga agatgggaaa cttgctaggt ttgaacaatc aacggtacaa gtatataaag
    19441 attttactgt tagaggtact aaaaactcaa ccgtaccaac agaacattat ggacaacgac
    19501 tattaaacgc ttatgaaact ccagaatatt atttcgctga ttatggggaa gccgttacag
    19561 gtgatgatgg taaagttcgt gttgatattg accccatgtt tgctgagaca gtaaatctaa
    19621 gtcgatatat gacacatgtg acacctacag aactagtttt gtgtgctgtt actcatgaag
    19681 atattgacca tttcatcatt gaaactagta agccaaacgt attagttaga tggaatttag
    19741 tggcacaccg tctagggtat gaagatatta gattaaaaga ggatacagca tatgatagca
    19801 cagtgcttga ccaaaaacgt ttttaaaacg aagacaagga ggtatataaa tggctagcag
    19861 tttatataat ttggctctag atttcagcaa agaattaaac tacaccaaag ctattatggc
    19921 tcgtcaaggt gataaaggga ttacagtgac tgttaaacca tttttaaatg gcttgcagat
    19981 ggatacgagt ggcggaacat ttactttaaa aggaacaacg ccatctaacc gttacgtaga
    20041 tagtgtggca actagtgtaa ctagtgaaga agtcacgttt tctcttgatg gcacatttat
    20101 gagtgaagca ggatattata aacactgcta cgtagaatat agaaaagaca atcaaatttt
    20161 aacaacgcaa gatatcattt ttttctcact aggagtgtct gacatttcgc aaggccaagc
    20221 cgatgaatac gtttcgcaat tggaagagtt gattcaaaag tataacgaaa cttttgatgc
    20281 ttttatggct gaaatcgaag gtagagtgga cagcttaaat aaacagatta ctgatttaac
    20341 tggtcaagct aaaacactac aagacaagtt agacgctctg aaagaagaaa tttctaaatt
    20401 aggtaacttg caggtgatgt acagtaacag catcga
//
LOCUS       DAHUWM010000029        20420 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHUWM010000029 DAHUWM010000000
VERSION     DAHUWM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.029"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            20..1099
                     /locus_tag="NK788_000755"
     CDS             20..1099
                     /locus_tag="NK788_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6769103.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1296..1997)
                     /locus_tag="NK788_000756"
                     /pseudo
     CDS             complement(1296..1997)
                     /locus_tag="NK788_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2011..2935)
                     /locus_tag="NK788_000757"
                     /pseudo
     CDS             complement(2011..2935)
                     /locus_tag="NK788_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3052..4131
                     /locus_tag="NK788_000758"
     CDS             3052..4131
                     /locus_tag="NK788_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCH6769104.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4163..4756
                     /locus_tag="NK788_000759"
     CDS             4163..4756
                     /locus_tag="NK788_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6769105.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4866..5093)
                     /locus_tag="NK788_000760"
     CDS             complement(4866..5093)
                     /locus_tag="NK788_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6769106.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5196..7784)
                     /locus_tag="NK788_000761"
     CDS             complement(5196..7784)
                     /locus_tag="NK788_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCH6769107.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7804..8463)
                     /locus_tag="NK788_000762"
     CDS             complement(7804..8463)
                     /locus_tag="NK788_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6769108.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8512..9015)
                     /gene="trmL"
                     /locus_tag="NK788_000763"
     CDS             complement(8512..9015)
                     /gene="trmL"
                     /locus_tag="NK788_000763"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCH6769109.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9236..10102)
                     /locus_tag="NK788_000764"
     CDS             complement(9236..10102)
                     /locus_tag="NK788_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6769110.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10211..10852
                     /locus_tag="NK788_000765"
     CDS             10211..10852
                     /locus_tag="NK788_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCH6769111.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10914..12275)
                     /gene="mgtE"
                     /locus_tag="NK788_000766"
     CDS             complement(10914..12275)
                     /gene="mgtE"
                     /locus_tag="NK788_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCH6769112.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12293..13192)
                     /locus_tag="NK788_000767"
     CDS             complement(12293..13192)
                     /locus_tag="NK788_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCH6769113.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13194..13991)
                     /locus_tag="NK788_000768"
     CDS             complement(13194..13991)
                     /locus_tag="NK788_000768"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCH6769114.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13969..14673)
                     /locus_tag="NK788_000769"
     CDS             complement(13969..14673)
                     /locus_tag="NK788_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCH6769115.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14804..15379
                     /locus_tag="NK788_000770"
     CDS             14804..15379
                     /locus_tag="NK788_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCH6769116.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15497..16174
                     /locus_tag="NK788_000771"
     CDS             15497..16174
                     /locus_tag="NK788_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCH6769117.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16253..18061)
                     /gene="pepF"
                     /locus_tag="NK788_000772"
     CDS             complement(16253..18061)
                     /gene="pepF"
                     /locus_tag="NK788_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCH6769118.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSGSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18237..19235)
                     /locus_tag="NK788_000773"
     CDS             complement(18237..19235)
                     /locus_tag="NK788_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCH6769119.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19461..20114)
                     /locus_tag="NK788_000774"
     CDS             complement(19461..20114)
                     /locus_tag="NK788_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCH6769120.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5911 a         4442 c         3640 g         6427 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa
       61 acatctttcc tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa
      121 ccgtcaggtt gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg
      181 cggaaccatc acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta
      241 ttctgtttat gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg
      301 cagacgcccc aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt
      361 aaatgaaaaa tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga
      421 ttgtgccatc attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg
      481 aacaaagaac atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg
      541 acaccgtccc aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga
      601 aacacgggaa acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt
      721 gaacgggaag gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg
      781 cgctaacttc gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg
      841 gctacacgat gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg
      901 ggagcgtggg acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag
      961 cccgattagt gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta
     1021 cccgcggaaa attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca
     1081 agaacggttg gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct
     1141 tcatgtctgg aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca
     1201 ttatcaaggc ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta
     1261 cctgtagtgg ctaacttaaa cttgaaattt acggacgtaa agcttgctgc caaattagcg
     1321 attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga
     1381 tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt
     1441 ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct
     1501 tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact
     1561 tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc
     1621 atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg
     1681 agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt
     1741 tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa
     1801 ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcgaga
     1861 ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga
     1921 aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta
     1981 taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc
     2041 taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc
     2101 ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg
     2161 gagctgacct tttttataag cgattccatc agaagctatt gttcgctgac aacacgtttt
     2221 ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag
     2281 attctttggt tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa
     2341 gaatttctac ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca
     2401 gctccttgat actttctaaa attttattcc gactttgggg gtctacagct actgtcggct
     2461 catctaaaaa aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt
     2521 tcaagccacc actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt
     2581 gaatcacctc ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa
     2641 aataatcgat attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga
     2701 ccccaatcat tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa
     2761 tggttccttt gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac
     2821 tgccgtttgg tcctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat
     2881 tcaatgctac aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga
     2941 aaaactcctt ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag
     3001 tgacgattgg ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa
     3061 tctcggctac tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt
     3121 caaggattcg ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc
     3181 agttatttac tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt
     3241 gcctttttac ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa
     3301 gaaagccacc ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat
     3361 catcccccca tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga
     3421 aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat
     3481 tgggaaaaac aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca
     3541 caattagcct tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat
     3601 gtcggtcatc tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa
     3661 gaaccaaaac taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat
     3721 cggattcgtc aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta
     3781 gagtttctga tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga
     3841 ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt
     3901 atcaaacata gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat
     3961 cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt
     4021 ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa
     4081 aaccagcaat ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag
     4141 tcattattga gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa
     4201 agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac
     4261 ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc
     4321 tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa
     4381 agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca
     4441 gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg
     4501 taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca
     4561 tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa
     4621 aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat
     4681 cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta
     4741 tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc
     4801 attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga
     4861 aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga
     4921 aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct
     4981 acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca
     5041 ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa
     5101 cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac
     5161 caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa
     5221 tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact
     5281 tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct
     5341 ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca
     5401 ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta
     5461 tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg
     5521 gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc
     5581 cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc
     5641 aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga
     5701 tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt
     5761 gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt
     5821 gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata
     5881 cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg
     5941 aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt
     6001 ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc
     6061 cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt
     6121 tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga
     6181 tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga
     6241 ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt
     6301 tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct
     6361 ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc
     6421 agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc
     6481 gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg
     6541 gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt
     6601 agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct
     6661 gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt
     6721 aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac
     6781 tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa
     6841 tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa
     6901 aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca
     6961 agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct
     7021 aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga
     7081 actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct
     7141 gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct
     7201 tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg
     7261 tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga
     7321 tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa
     7381 ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc
     7441 agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa
     7501 agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta
     7561 agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt
     7621 tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg
     7681 gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc
     7741 gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt
     7801 cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac
     7861 tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact
     7921 attgaacaat ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat
     7981 ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt tatgttgggc
     8041 aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg
     8101 aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata
     8161 tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag
     8221 cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt
     8281 atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt
     8341 tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct
     8401 cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc
     8461 catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag
     8521 cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc
     8581 ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt
     8641 catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt
     8701 tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata
     8761 tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa
     8821 agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg
     8881 ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt
     8941 cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa
     9001 aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca
     9061 atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac
     9121 gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa
     9181 aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa
     9241 aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct
     9301 tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag
     9361 gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac
     9421 gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat
     9481 aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt
     9541 cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt
     9601 aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat
     9661 tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc
     9721 cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat
     9781 tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct
     9841 gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa
     9901 cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt
     9961 ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta
    10021 cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc
    10081 ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg
    10141 ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg
    10201 agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat
    10261 tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc
    10321 gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat
    10381 gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat
    10441 gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct
    10501 tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg
    10561 gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc
    10621 aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga
    10681 caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg
    10741 cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga
    10801 attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa
    10861 acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata
    10921 aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc
    10981 aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc
    11041 atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct
    11101 atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc
    11161 cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat
    11221 ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt
    11281 ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca
    11341 cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacccaa aaacaacaga
    11401 gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc
    11461 acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg
    11521 atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct
    11581 aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact
    11641 gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg
    11701 tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag
    11761 attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga
    11821 ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa
    11881 tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca
    11941 atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa
    12001 agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg
    12061 tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc
    12121 agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc
    12181 tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg
    12241 gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt
    12301 caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact
    12361 cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc
    12421 ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa
    12481 aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt
    12541 ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca
    12601 ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg
    12661 ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac
    12721 gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaaaccag
    12781 atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt
    12841 tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag
    12901 acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga
    12961 tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta
    13021 ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt
    13081 gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa
    13141 aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc
    13201 tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa
    13261 tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc
    13321 aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc tagccattca
    13381 tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca
    13441 atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta
    13501 ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca
    13561 aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt
    13621 gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca
    13681 tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct
    13741 tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag
    13801 aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct
    13861 ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct
    13921 aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc
    13981 gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta
    14041 gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc
    14101 acggatactg gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg
    14161 ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt
    14221 tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc
    14281 gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt
    14341 tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat
    14401 ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc
    14461 agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat
    14521 actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc
    14581 acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata
    14641 tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt
    14701 cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca
    14761 aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga
    14821 aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca
    14881 attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca
    14941 gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac
    15001 attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga
    15061 agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact
    15121 ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg
    15181 ggctgaattc cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct
    15241 acacgatttt gaactggaac tagaggtagg ggatgcatct gtgggaaaaa ttgcttttat
    15301 aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat
    15361 gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta
    15421 cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat
    15481 tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg
    15541 tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa
    15601 agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat
    15661 gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac
    15721 ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg
    15781 ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact
    15841 tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca
    15901 gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat
    15961 cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt
    16021 agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga
    16081 taactcatat caagttttta atggtgaaca tttacataca aatgattctc agttccattc
    16141 aagaactagc catttacact tgctttctaa ttaaaaaaat acgtcgactc ccttacaaga
    16201 gagccgacgt atttttttcg tttctattcg agaaaatcaa aaatagaaag ccttataaac
    16261 gatcgatcaa ttcttctaat tcatttagac gttgttcaaa catcgacatt gcatcttcaa
    16321 tatatgcagc ctgtgtcata tcaactcctg cttttttcat tacttccaca ggataatcgc
    16381 tgttacctgc ttttaggtaa gccaagtagt tctctagtgc ctctggttcc tggtttagta
    16441 ttttcttcgc cagtgctgaa gcagcagaaa agccagtcga gtattggaaa acatagtaat
    16501 tataataaaa atgcggtatc cgtgaccatt cgaatttaat ttctgggtct tcttcgactg
    16561 ctggaccata atattttgca ttcaatttac cataactacc acttaggtat tcactagtca
    16621 atggcacacc tttttcatct tccgtatgca tgaaatgctc gaattcagcg aattgtgtct
    16681 ggcggaaaac tgttccttta aacccatcca agtaatggtt gagtacataa gcccgtacac
    16741 gagggtcttt ttctgtttcc aataaatact ccgttaggat attttcattc gttgtcgaag
    16801 caatttctgc taaaaagatg gagtagtcgc cgtacacata aggctggttc gaacgagtga
    16861 aataactatg aacactatgt cccatttcgt ggacaagcgt aaatagctga tccagcgtat
    16921 catgccaatt caataaaata tatggatttg tgtcatagct tcccgaagaa taagcaccgc
    16981 ttcgtttccc tttattttcg acaacatcga tccaacgttc agagaatgct ttttctacga
    17041 tggccatgta ttcttcaccc attggtttca gtgcttctaa agctttttct tttgcttctt
    17101 cgtacgtaaa ggtaattgga gcttccccta agaccggtgt ataaagatca tacatgtgca
    17161 gtttttccac ttctaataaa cgtttccgta attccatgta tcgatgtaac aaaggcaaat
    17221 gcttgtttac cacgtctacc aaagtatcgt atacactttc aggaatatga ttattgctca
    17281 aagacgcttc tctggcagag ctgtaattac ggactttcgc tttaaaatta tgtcctttta
    17341 tatgtgtgcc taaagtagaa gcaaatgtat ttctaaattg ttcgtaaaca ctgtacaatc
    17401 ccttaaatgc tgcttcgcgc acccttcgat ccgtgctttc tagcaactga ccatacacgc
    17461 catgagataa ttggactatt tcaccatttt ctccttcaat cgttggaaaa actagatctg
    17521 cattattcaa aacagcaaat gtatcacttg aagcatcaaa gatttcacct gctccagcaa
    17581 gaagagattc ctgctcagca gataagacat gggctcgatt atctactatt tgctggatat
    17641 aatggcgata gacttccaat tttggttctt ctttgaaata ttgccaaatc tggtcatctg
    17701 acaattgcaa tatttctggt tcaaaccagg aaacggcttc actaactttc gaaaacagac
    17761 tgcttgctct tgcataaagc gcttggtaat ctgtatttcc agtgtcttga tcgtttttaa
    17821 ggtgcgcata tacataaatg acttcagttt ggcgataaac cctcaatacg aattcaatcg
    17881 catttaaaaa ttgagaagcg ccttgatcaa gtgttccttt gtgtttttca gattgtttta
    17941 actcttctga taattccaaa tatttctcat caaactcttg gtcgcttgaa aagatcttgg
    18001 tcaagtccca agttaaattt tcaggcaatt cttctcgttt tggcaactgc tttacttcca
    18061 tgaaatccac tccttttctt cccttagctc tatctatcaa aatagatcat agtctaacat
    18121 ccacatgtca cagcatctaa cttttctact cattcatgtg tctgcttcca tcaattttag
    18181 cataactaaa tcttgtttta catagaaaac cgattcctag cctattttta tacttactat
    18241 tttttcaaag aagacaagtg tatgcattcg ttaaaaaact gctgatagac catctcttca
    18301 tcaatcatag gaaaaagcca ttccctttta tgttctttta tttggcaaag ccatgagttg
    18361 aaatcaggaa ctttctggtt ttccaaggct tcttcgtaca acattcgata aagaaaaacc
    18421 tgttcttgta aataaaagaa aaaccgactt ttttgataga tccacgatga tagatgcaaa
    18481 agatgtttat gacgcaagta gcattgttct tgtatggata ctgtattctt ttgcttgtga
    18541 ataagttggc gtgtcagcca tttcttccca tcttcactca ctttttctat ttttttcatt
    18601 tggttcattt taatttcgtt actagtgaat atttctttaa ggtccctact agaaaaagtc
    18661 cagcagctac tgcaaaagga aatatgccct gatacagtct ctgtaatatg atgatacaaa
    18721 taaagctttt gtttcttgag atcagctttc cagcaatgca ctcctctttt tctattatag
    18781 tagcagcaac ttttttctat caatgagaat tgcgattgtc caagaaaaga attaccaagt
    18841 atccaccata cttggtaact gttattcaaa taattttggg ttctttcaat aaatctctgg
    18901 tgagacagtc tagaacattg gatttctatt gctagattcg ccaacaaaat atccggcctt
    18961 tgccgcaacc tcggcaaata ggcttccaaa aatactggtt ctactgactg tcccagccaa
    19021 tccatgaagc attcctttaa ctgtaagtgc tcttttgatt ctctttcact aaagaccgaa
    19081 cagttatctg atttttgatg ggcaaaatga gcacattttt tctttccctt tttcaaaatc
    19141 actctttttc gacagccacg acaataatat ttctttcctt tttctgcatg tttagcaaaa
    19201 cagcacttat tatattcatt cattgcaagc agcatttttc tccctctgaa cttatttacg
    19261 tctaagaatt tatttacacg aaaaaagagg gcgtgaaaaa gctattctgc atcaagtaat
    19321 aagaacagct aaacaaaaat agttcccaat atttttcgtt tggttgggct tgttaccgca
    19381 gatgcaagct tttgaacaca tttatacgaa aaaaagactg cgacacaatt tttgtcacag
    19441 tctcttacta taatgctgat ttatacaaaa taaaatttcg ttcgctcgat agcattacgc
    19501 gccatcagac attgtccata ttcattcaat gtttctggtg tacgatcgct cttttcagca
    19561 aactccaaaa ttcgagcaat ctgattctct acatccgctt ggttgagatt ttccatccag
    19621 aagtgaacga ctaggtaata gcgctcttcg aataaataca aatcagtcca agcagactct
    19681 agttcgacat ctgttgcaag ttggatcact tgttcaaaat cttcaaaagc aaaagttgct
    19741 tgtctttcct gttcttctag ggcgcttcca gataattctt cctccggatc gctgccaata
    19801 atctgctgac gaagaaagtc actcacatct tctgaatcaa cagtagaatc ttctgattga
    19861 gaaatttctt catccggaag atttttactg ataaacagtt ctaatccgtc tccttttggc
    19921 aaaacttgga aagtgacagc ttcgcttcct cggaactcct cttcgatatc aacttcttcc
    19981 aaaatactat aaaagaaatt ttcgatttct ttgtgattgc caagtaaatc caaaaatgaa
    20041 acgccccgtt cctctaaatc ttcatgacca attagtacac ggatcgtatt ttcattaata
    20101 tgttccattt ccattttgct acacctcact ttgccacaat gtttaatata gattcattgt
    20161 agcgaaatca gcttaaatgt aaagaaaaaa aatctcgctg ttaagcgaat ttaataaaac
    20221 cagcggattt ttcaaccttt caagctatct tatttaacta taacattttt ggcagacgaa
    20281 tactatatag ccgacttttc agctaatttt ccctctctat ttttcgtgca atcatcatct
    20341 tctactacct ttttatacat ctaatcaaga atactttttg taaaaacaaa aaaacttgga
    20401 aaaactatct ttgtaatagt
//
LOCUS       DAHUWM010000030        20165 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHUWM010000030 DAHUWM010000000
VERSION     DAHUWM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.030"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            43..3432
                     /locus_tag="NK788_000775"
     CDS             43..3432
                     /locus_tag="NK788_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCH6769121.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3435..4856
                     /locus_tag="NK788_000776"
     CDS             3435..4856
                     /locus_tag="NK788_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6769122.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4853..6730
                     /locus_tag="NK788_000777"
     CDS             4853..6730
                     /locus_tag="NK788_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6769123.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6822..7664
                     /locus_tag="NK788_000778"
     CDS             6822..7664
                     /locus_tag="NK788_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6769124.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7816..8691)
                     /locus_tag="NK788_000779"
     CDS             complement(7816..8691)
                     /locus_tag="NK788_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6769125.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8898..9929
                     /locus_tag="NK788_000780"
     CDS             8898..9929
                     /locus_tag="NK788_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6769126.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10018..10323
                     /locus_tag="NK788_000781"
     CDS             10018..10323
                     /locus_tag="NK788_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCH6769127.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10502..12517
                     /locus_tag="NK788_000782"
     CDS             10502..12517
                     /locus_tag="NK788_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6769128.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12519..12803
                     /locus_tag="NK788_000783"
     CDS             12519..12803
                     /locus_tag="NK788_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6769129.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12829..14202
                     /locus_tag="NK788_000784"
     CDS             12829..14202
                     /locus_tag="NK788_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCH6769130.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14285..16300)
                     /locus_tag="NK788_000785"
     CDS             complement(14285..16300)
                     /locus_tag="NK788_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCH6769131.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16322..17536)
                     /locus_tag="NK788_000786"
     CDS             complement(16322..17536)
                     /locus_tag="NK788_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6769132.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17757..18701
                     /locus_tag="NK788_000787"
     CDS             17757..18701
                     /locus_tag="NK788_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769133.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18997..19941
                     /locus_tag="NK788_000788"
     CDS             18997..19941
                     /locus_tag="NK788_000788"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCH6769134.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6880 a         3707 c         3852 g         5726 t
ORIGIN      
        1 ctattcacgt caaaacagaa aaaaagaaaa aggaggggaa aaatgacgac cacagggaag
       61 aaactgaaag ttattttcat gctgataata ttgagtttat caaactttgt gccattatct
      121 gcaatagcag acactacaga tgatccaaca gttttagaaa caatttcagc tgaagtcatt
      181 tcggatcagt ctggaaaaaa agcactgaac atcaagctaa atgcgaataa caccagtgct
      241 gaaaagatag aaaaagaaat tggtctagtc gaaaattact taagtgatgt ggaaagaaaa
      301 gaaggagatg gctatgctta tcaggtaaat agcgggaaaa ttacgttgga aatctcatca
      361 aacactaaac aaactatcga tctgagtttt ccaatcgatc cagcacttta ccacagccag
      421 gcaaacaagc tgatcgtcga taataaagaa tatgacatta ttgatgagac agaaaataag
      481 aaagatacag atgtgtcagt accaaagcca gacgaaatag aagaagaatc atcaaaagaa
      541 aacgaaaatt ctgtcagccc atttacattg cctacattat ccttgccagc tgtgagtgtg
      601 ccatctaatc aaacgattcc tacagaatat acaacagatg atcagggcac ttatcctaaa
      661 gccagttggc aacctacagg aaatacaaat gttcttgatc atcaaggcaa taaaaacgga
      721 acaaatcaat gggatggtat aaattcttgg aatggagatc ctaatgatcg gacccattcg
      781 tatatcgaat atggaggaac cggtaatcaa gcagactatg cgatacgaaa gtatgcaaag
      841 gaaacaagta cacccggatt gtttgatgtt tatttgaatg ctcgtggaaa tgtacaaaaa
      901 gatatcacgc ctcttgatct cgtattggtc gtagactggt caggaagtat gaacgacaat
      961 aatcggatcg gtgaagtaaa gattggtgtc gatcgttttg tcgatacttt agcagatagc
     1021 ggtatcacag acaaaatcaa tatgggatat gtcggctact caagcgaagg atatagctac
     1081 agtaacggtg cagtacagat gggttcattt gattcagtga aaaatcaagt aaaatccatt
     1141 acaccttcac ggacaaatgg tggtactttt acacaaaaag cactaagaga tgcaggaagc
     1201 atgctatccg ttccaaatgg acataaaaaa gtgatcgttt tgctgacgga tggtgtacca
     1261 acattttcct ataaagtaca gcgggtacac gcacaatcaa gcagcaatta ttacggaact
     1321 cagttttcta atacgcaaga tcggccggga aatacttctc taatctcaag aatctatgat
     1381 gcacctgacc aaaacaatct atccagaaga atcgacagta cgtttatcgc aaccatcgga
     1441 gaagcgatgg cactcaaaga acgaggaatc gaaatacatg gtcttggcat ccaacttcaa
     1501 agcgatccgg cagctggtct ctcaaaagca gaagtagagt ctcgtatgcg acaaatggtt
     1561 tcatcagatg aaaaaggcga tctttactat gaatcagctg atcatgcaac agatatctct
     1621 gaatacctag ccaaaaaagc tgtacagatc tcagcaactg taagcaatgg acaaataaat
     1681 gatccaatcg cagaaccatt catttatcag cctggtacac tttcagtcaa gagtgtgggg
     1741 acaagtccta caacggtcac tccatctatt tccatagaag gaaataccat caagagcaat
     1801 cagatctatt taggaaaaga ccaagaaatc caaatccatt accaagtgag aatccaaaca
     1861 gaaaatgagg acttccatcc aaatttctgg tatcaaatga acggcaggac aactttccag
     1921 ccaaacattg ataccaatga attagctgaa ttcggtatac catctgctaa agctcccgga
     1981 gtcagtcttc acatcaaaaa gttatgggaa gaatttgaca acaatctagc tgatcgtcca
     2041 gatcaagtta cttttgagat tcaacgggaa catacgacaa atgctgcagc ttggaaaaac
     2101 ggatatattc gaatcattaa accagctaaa gatacaacaa atacgtggga acgtgcagac
     2161 attgacaaat tatctgcaaa tagcggagaa agttatcaag agatattatc actacctcaa
     2221 tacaataatc aaggtcaagc attcagttac caaacaatca aagaattacc tgtaccagga
     2281 tacgattctc aacaaataga tgcaatgaca tggaaaaata ctaaacaatt cacaccgtta
     2341 aacttgaaaa taacgaaaaa ttcctctaca ggtgaaaagg atcttattgg cgctgttttc
     2401 aaattaacag gagattctat tgatacttta ctaacagatc atggcgacgg aacctattct
     2461 cttccagaaa atgtcaaatt gcaaaaagaa atgacctata cgctgacaga aacaaaagct
     2521 ccagaagggc atggattaag caaaaagact acttgggaaa tcaagatcgc ttctgatggt
     2581 acggtaacca ttgatggaaa aacagtcact acttccgatg atacgatcca gttgactatt
     2641 gaaaatcctt ttgttgaagt tcctgtagca gtacgtaagt atgcgatgca agggacggac
     2701 aaagagataa atcttaaagg agcagcattt tccctacaga aaaaagaagc aaatggtact
     2761 tatcagccaa ttgacagcca aacaacgaat gaaaaaggtc ttgccagttt tgattcactc
     2821 acacctggta aatatcgagt cgttgaaaca gctggtcctg ccggatatga tacttcgccg
     2881 ggaaattatg aattccaaat cgataaatat ggaaaaatca tttacacggg aaaaaatacc
     2941 gagatgacaa ataatgtatg gacgctcact catcaaaatc gactaaaagc gtttgatcta
     3001 acggtacaca aaaaagaaga caacggacag acattaaaag gagcaaaatt cagactgcag
     3061 ggaccagaaa tggacttaga atcgccaaaa gatggacaag aaacagatac ctttctattc
     3121 gaaaatttaa aacctggaac ttatacgctg accgaaactt ttacaccaga aggataccaa
     3181 ggtctaaaag agccagttac tatagttata cacgaagatg ggtcaattca agtggatgga
     3241 caagatcatg aatctgttct gtcaccagga gccaaaaaca accagatttc tttagacatc
     3301 acgaatcagg caaaagtacc attacctgaa acgggaggaa ttggccgttt aggaatctat
     3361 ctagtaggga tgattggttg tgcgttttct atttggtatc tttttttgaa aaaagaaaga
     3421 gggggcagct aaatatgaaa aaacttggtt ggcttagtat gtgtctcttc ttgttactat
     3481 ttaaaccagc ttttactcag gtagcaacag aaacagaaac agaaatggtt cagattactt
     3541 tacacaaatt gcttttccca aacgggcaac tgccgaaaaa tcatccaaat gacggacaag
     3601 aaaaagcttt attacaaacg tatcgaggat taaatggtgt cacattccaa gtttatgatg
     3661 tcacagattc tttttaccat ctacgggaaa agggcaaaac ggtagaagaa gcacaagcag
     3721 agatcgcaaa aaacggtgcg tcttccggta tgtttaccgc agaagcaaca actacaactc
     3781 ttaacaacga agatggtatc gcttcttttt ctctggccgc taaagatcaa gaaaaaagag
     3841 ataaagcgta tcttttcatt gaatccaaag taccagaagt cgtcaaagaa aaggcagaga
     3901 atatggtagt tgttcttcct gtacatggac aaaacaatca aaaactttca actatccatt
     3961 tgtatcctaa aaatgaagaa aacgactacc ctgatccacc ttttgagaag gtattagaag
     4021 agcctagaaa tgattttacg attggtgaaa aaatcactta ttccttgcat acgacaattc
     4081 ctgtaaatat ccttgactat caaaagttcg aattgtcaga tagtgcggat gaagcattaa
     4141 cgtttttacc taatagttta acgatttcat cgaatggaga aaagctgaca gaaggctttg
     4201 tcatacacaa gaaacctcac ggatttgatg ttttattttc gatcccttcg ttggaaaaat
     4261 atgctggaaa aaaactgacc atttcttatc agatgcagct aagcagtaca gcacaggcga
     4321 acaaggaaat caacaacaac ggaacactgg attttggttt tggtgtcagt acaaagaaag
     4381 tctctgtata tacagggagt aagcaatttg tcaaaatcga gacaaataaa ccagataaac
     4441 gattagctgg cgcagtattc cttattaaaa acaaagcagg aaattacctc cagcaaacag
     4501 ccaacggata caagtggaca aagaacgaat cagatgcgct tcacctgatt tccgataaaa
     4561 atggcgcttt ttcaatttcc gggttgaaaa caggaagtta tcgattaaaa gagatcgaag
     4621 caccttctgg ttatatttta agtgaaacag aaattccgtt taccatttca acttttcttt
     4681 ctgaggataa agaggcggac agtatattga aagtagtcaa taaaaaagaa aatagccgtc
     4741 catttcttcc aaaaacaaac gaaacgaaaa atacactttt aggcgttgtt ggtatggtat
     4801 tcgcaagctt tgcaatctgg ttgtttatca aaaaaagaac aggagtgaaa aaatgaaaaa
     4861 tcataaaaaa ataaacgtta tgttaggagt ccttttcctt attttaccat tactcacaaa
     4921 cagcttcggc gcaaaaaaag tgtttgcaga ggagacagca gctcaagtca tccttcataa
     4981 aaagaaaatg actgatttac ccgatccttt aatccaaaac agcgggaaag aaatgagcga
     5041 attcgatcaa taccaaggat tagccgatat ttcattttca gtttataacg tcactcaaga
     5101 attttatgcg caacgagata aaggagcgtc cgtggatgca gcaaaacaag cagtccagtc
     5161 tttgactcct ggtacaccag ttgcttcagg aacgacagat gctgatggaa atgtcacttt
     5221 atctttacct aaaaaacaaa atgggaaaga tgcagtctac acgatcaaag aagaaccaaa
     5281 agacggagtg tcagctgccg caaacatggt tttagctttc cctgtatatg agatgatcaa
     5341 acaagcagat ggctcttata aatacgggac agaagaacta gatactatcc atctctaccc
     5401 taaaaataca gtcggtaatg atggaacgtt gaaagttaca aaaatcggta ctgccgaaaa
     5461 cgaagcacta aatggagcag aatttattat ttctaaagaa gaaggaacac caagcgtcaa
     5521 aaaatacatc caaagtgtca cagatggatt gtacacttgg acaactgatc aaaccaaagc
     5581 caaacatttc attactggtc attcttatga catcggcaac aatgactttg ccgaggcatc
     5641 tattgaaaaa ggccagttga tcgttaatca tttagaagtt ggaaaatata atttagaaga
     5701 agtaaaagct cctgataatg cggaaatgat tgaaaagcaa acaatcacgc cttttgagat
     5761 cctggcaaat agccaaacac cagtagaaaa gaccatcaaa aatgatacgt ctaaagttga
     5821 taaaacaaca cctcaattga atggaaaaga tgtcgcaatc ggtgaaaaaa ttcaatatga
     5881 gatttctgtc aatatcccat taggtatcgc tgataaagaa ggaacgcaaa acaagtacac
     5941 aacattcaaa cttatcgata ctcatgacgc tgctttaaca tttgataatg attcttcagg
     6001 aacgtatgct tatgccttat atgatggaaa taaagaaatc gacccagtaa attattctgt
     6061 cactgagcaa acagacggat tcacggtttc agttgatccg aattatattc cttcattaac
     6121 tcctggcggt acattgaaat tcgtttacta tatgcatttg aacgaaaaag cagatccaac
     6181 caaaggattt tctaaccaag caaatgtcga taacgggcat acaaatgatc aaacaccacc
     6241 gtcagtcgat gtcgttactg ggggcaaacg atttgttaaa gtagatggtg acgttacatc
     6301 agaccaaaca cttgctggag cagaattcgt cgttcgtgat caagatagtg acacagcgaa
     6361 atatttatcg atcgacccat ccacaaaagc cgtcagctgg gtatcggcga aagaatcagc
     6421 aacggttttt acaaccacaa gtaacggttt aatcgatgtg acaggtctaa aatatggcac
     6481 gtactatctg gaagaaacga aagcgccaga aaaatatgtt ccattaacaa accgtgtagc
     6541 atttactatc gatgaacaat cttatgtaac agcaggacag ttgatttctc ctgaaaaaat
     6601 accaaataaa cacaaaggta cacttccttc aacaggcggt aagggaatct atgtgtatat
     6661 cggtgcagga gtagtccttc tactgattgc tggactgtac tttgctagac gcaagcacag
     6721 tcagatttag tctttttgca agaattggaa aaaggagcag cggattacgc aaatgctcct
     6781 ttttctttta ttatcaaaca gtcatcacaa ggagaacaca aatgaaacca aagaaaagga
     6841 aacttctaga tctgctgatg cttttcttgc ttctctcagg aatagggata ctagcttatc
     6901 catttgttag cgacgcgctg aataattatc tggatcagca gatcatctct cattatcagc
     6961 aacaagcggt caaagaaaat gaagaagtca tggctaaaat ccaagaaaac atgacaaaga
     7021 aaaaccagca attagcaaaa gaaggtggaa atccaggtgc agatcccttt actaagaaaa
     7081 aagaacctgc gaaaaaggat agaacctatt ttgagaaaca tacgatcggt attttgacga
     7141 tccctgagat caatgtccat ttgccaattt ttgatgaaac gaatactgtg ctgctagaaa
     7201 aaggtgcctc attattggaa ggaacgtctt atcctacggg gggagaaaat acacatgccg
     7261 ttatttccag ccatcgagga ctgcctcaag caaagttgtt cacagactta ccgaaactga
     7321 ctttaggtga ttctttctat atcgaaatca atggacggac actcgcttat caagtcgatc
     7381 agatcaagac ggtagaaccg actgatacaa aagatctgag aatcgaaaaa gggcaggatt
     7441 tcgtgacact cctaacttgt acaccttata tggtcaacag tcatcgctta cttgtcagag
     7501 ggcaccgggt tccttatcat gcaaaagaag ttcaaaaaga agtacagaaa gtatccgaga
     7561 acaaacgatt tttcctgtat ggattgttag ctgcatgtat actgattttc ctccttctta
     7621 tatatctttt tcgtcaatcg ttcaaaataa gaaacagaag ttagctattc ctcatttcta
     7681 taatatatct attcgaatcg ttccgtaaac aaacaggctg aggtttgaaa aagaaaactt
     7741 cagcctgttt ttatgatgat atcaaagaga gcctatcaaa cactatcttt cttattacta
     7801 aatgaggatc ttttcttaag ttttccgata ttctttaaga aggccttttt gcatcaattc
     7861 ttgtttgttt ttcaagttgc ggatattctc ttcaaaattt tgagaatata tcactgagaa
     7921 taacgtattt aatacgtaat cgaaggcaat ttgagaggag aaagtcgcta ctttagcaaa
     7981 atcatattcc tcctgcgata taacaagaca catatcagcc tgtttggcca tttctgaatg
     8041 ctgatttccg gtaatcagca aggtaggggc ctctacatgc ttcaaatagg tcattattcg
     8101 ttcataatga tggatgcgac cgctgtagct aataaaaatg gcgcaatcct cgctagtcaa
     8161 attggctgca ttccaagaag aatcactgta ttcttcagcc atgatcaaaa acttattcaa
     8221 cttgaccatt ttattctgga atttccttgc cgtgatttga gaatcacctt ttgcaaaaag
     8281 aaagattcgc tgtgccttgt tcaatacttt accgatttga tctaaatctt catgttcaat
     8341 ctgcaattgt gcttttttta ttgactggat cgataaatcc gccattttct ttgcaatttg
     8401 ctgagtgcta tcttcacttg aaaaaggaaa attaggatca atcgtttgat tgaactgagc
     8461 aatcaattga tgaacttccc gccccagctc gaaacggaat tctttaaatc cgctgaatcc
     8521 catttttttg cataaacgaa tgatagctga atgggaagta tacgtagctt ttgctaattc
     8581 ttcaatcgtc atgtagactg cagcttcgat attcctacgg atgtaatcga caatccgttt
     8641 ctctgtcgtg gtaaacaact cttgttttgt tagtttttct tcaataagca tgatctgacc
     8701 tccaaattct atcttactga taaacacttg gttccgctac tatctttttg atttttatcc
     8761 ttactttgaa cattaatttc accaaaccga atgataacaa gtgtttttct gaactttttt
     8821 accagaatag ctattataaa tactttctgc gcttttctag gatatactga aaacatcaat
     8881 aaaaggaggc accactcatg cttacaatcg cttatcttgg caatggaaag agtacaaatc
     8941 gctatcattt gcctttttca agaaaactta cgaacaaaat caatatcaaa aaaatttttt
     9001 ccccatctga accagtgtgg aaaacattcg aagacatcga atatactgat cagctgtctg
     9061 atctttggga tgatcctgag atccaattag ttgtcatcac tacacctagt caatttcatt
     9121 atgaatatgg aaaaatggca ctcgaacatg gtaagaatgt actagtagag aaaccttttg
     9181 cagaaacgtc aaaggaagca gaagagttat ttgcattagc aaaagaaaaa ggtctattta
     9241 tccaatgtta ccaaaacaga cggttcgatt ctgacttctt gacagtccaa aaagtaatcg
     9301 aaagcggaaa attaggggat attttagaag tagaaatgca ttatgattat ttcagaccgg
     9361 aagtaccaga aaatacgcat gaattttcta ctgcgaacag ctatttatat ggacatgctt
     9421 gtcatacagt agatcaagtt ctttcttatt tcggcgaacc agatgatatc agctatgatg
     9481 tacgtcaact tttagggaaa ggacgaatga atgattactt cgatctggat tttttctatg
     9541 gaaatatgaa aatttcgata aaatccagtt atttccgcat caaagaacgt ccaagtttcg
     9601 tcgtttacgg aaaaaaaggg atgttcgtga aacaaacaaa agatcgacaa gaagaacatc
     9661 tgaaaatgtt ttacatgcct gatcatgaag actttggtat cgatttgccc gaacattatg
     9721 gtgtattgac ctatatagat gaacaaggga gttatcatga agaaaaagta atttccgagg
     9781 ttggtgatta cagccgtgtc tacgaaggga tttacgagac attagttaat ggcgcagaaa
     9841 aagtcatcaa agatgaagaa acaatcttac aaatgaaaat tttggaagaa ggaatcaaaa
     9901 gcattcaaga atcagaagga aaaatatgac atatttgtaa gaatcgggtt tgttttttga
     9961 cgcataaact gtcttattgt acgatgaaaa agtatagaac tactaggagg aaccaacatg
    10021 acaaattatg acggtgaaaa acacaaacga ttaagaggtt caatggcccc taaaacagaa
    10081 gaagaagtgg ctttagcagg aactaattct ggtgaaacag aagacaaaga agaatcatta
    10141 gctggcacaa actctggtga aacagaaaat aaagaaacag ctttatctgg ttcaaattct
    10201 gtagaagagg aaacaaagcc taatgagccc aatcactttg aaaaagatat cgaaaaagcg
    10261 gttgaagatg tcaaagaaaa atttgaagaa ctgacctcaa aaaataaaca tgatcatcat
    10321 tgatcggacg aaatcaattc ataaaagcag gagacatttg gtcacctgct ttttttctgt
    10381 atccattcga tagaaatcgt tcggtacagt cgtaaaaaaa atccaactgt aagttgtacc
    10441 aaacacccct cgatttaaaa gcgctaccat actagaatat aagtaggagg tgaacagcgc
    10501 tgtgaactac aaaattagtt atctcctgaa aacaccaaga gaaatcagtc tagaaaaagc
    10561 agcagagatc atcgaagtaa atacaagcga ggcgatcaaa caattaactc cttttgccga
    10621 atatgtggat gatacccgta tccgtataaa gaaattccga gcaatcgact gggcagaaaa
    10681 atttttggat attactcgat ttgaagctgt gtataccgaa caagagcgaa gagatcttct
    10741 ttatttgatg gtttattcga ctgcaacgtc tgtttctgtt tttcattttc aagattttct
    10801 tcaggtttct aaagggacag tcctgtcaga tatcaaaaag ataagaaatg aattagaagt
    10861 ggaagattgt cagctgtcgt atactaggaa aactggtttt aggctgctag ggtcagaagc
    10921 aatattaaga agagaagcaa aaaatcgcac tgctcgtttg atgcagtcac ttgctggaag
    10981 atttgggctc acgtatttaa ccgcaaccct ttccattgat tattacgcca aagttagaga
    11041 tttgctagat aaaacgattt cactatcaga tcttcgattc gcaccaaatc gaatggatga
    11101 attagcttat tatttgagtt tgacaaccat tcgaattgga aaaacatcca tggaactggt
    11161 aaaagatgct gaactgttgg cttctcttgc agtcactgac attagtcgac aagtattgac
    11221 tggccttggc tattccgaac atcaaaattc ggagattata tttttctctc tttgcttagc
    11281 atcgatcgtt gaaggagatg tccaggagcc tgcacttgat tttttgctgg aatgttcttc
    11341 agaagtcatc catcgcatgg aattcctgat ggccgtagaa tttgcttcgt ttcgtgaatt
    11401 attgatgaat gtcttttatc acttggtccc tgcctatttt cgtatttcct attcttttta
    11461 tttacctaat gtgatgatcg atcaaattaa gcatcaatat gcttccatct acgaaatgac
    11521 aagaaaagca ttacttccgt tagaaagacg aatagggaag agcatccctg aagaagagat
    11581 tggttttttc actatactat ttggcggaga gatcagaaaa gcggacgcag aagaaagaaa
    11641 tagaaaaata agagccgtga tcgtctgtcc aagtggcatt agctcttcat tgatcttaaa
    11701 aagtgaactt cagcaattat tcccgatgat cttttttaca gagactaatt cagctgaccg
    11761 cttacacgag gttgacgaaa cttcttacga tattattttt tctactgtac ctctcacact
    11821 tactgataag aaaaagtctc tctatgttct gcatccaatc atgactgact tggaaaaaaa
    11881 tcagctgatc aatcaagtac aaagagattg gctgatacca ggattttcaa tgccttctgc
    11941 ccaagaattg ttgtcggcgc tactgcctta tatcgaatta aaagaaggcg tagacgaaga
    12001 aaagctctat caagtattaa atcgtaaaat gaacaaactt ttagaaaaga aggaggatca
    12061 acgtcctatg ctatctgaat tattaacaga agaaatgatc caattatcgg atcaaccaaa
    12121 aaactggcaa gaagcaatca catttgccgc acagccgcta ttatcagaaa ataaaataga
    12181 agaaagttat atcaaagcta tgatcgagcg cgtggagcag tatggtgcgt ttatccatat
    12241 tggcgatcac atcgcattac ctcatgctcg tccagaagat ggcgtaaacg aagtagggat
    12301 gtctttgctg aaacttcaac aacctgtgga tctgcttgat aatccagccc atcccattag
    12361 tttgttcatc tgtttagctg ctatcgataa tgaggcacat cttcgggctt tggcaaattt
    12421 aacaaagcta ctttctaata aacaaaatct tcaagattta ttacacgcat caacaaaaga
    12481 agaaatatta gcgatcataa gaaaaggaga cgaatgatat gaaaattttt gcagtatgtc
    12541 aaagtggatt aggaacaagt tttatggtag agatgaacat caaacaagca cttgcagatc
    12601 ttggagcaga tgtggacgag tttgaagtaa gtcatacgga tgttggaagt gtatctagcg
    12661 atatggctga ttatttcttc attgagcatt ctttgaaaga tacgctaact tctatacctg
    12721 atgaaaagct cgtacctttg caatcgatca ttgacagcga agaagtaaaa gaaaaagtaa
    12781 cgaagatctt gtcaaaagaa taaaaatgat tcaagggagg aactatcaat ggataaattg
    12841 ttgaatttgt ttgtgtcaat cgtcagtcag ccctccatcc tcgtttcttt gattgcactg
    12901 cttggtctcg ttcttcagaa gaagaaattt tcagaagtga tccaaggaac tatcaaaaca
    12961 tttgtcggat tcctagtact gatcggcggt gctggtttaa tcagtaattc actaacccct
    13021 tttgcttcga tgttccaaac agctctgcat actcaaggag tagttcctag caatgaagca
    13081 gtagtggcga ttgctttgca acaatacggt acacctactg ctttgatcat gctagtaggg
    13141 atgatcgtga atattttact tgctagattc acaagatata aattcatttt tcttacaggt
    13201 caagcaatgc tttatgtttc ttgtctaaca gcagtcattt tagtaagcgc ggggtttact
    13261 gcaaatttgt ggatgatctt attaggcggg gtcttcgaag gaacactatt aactattacg
    13321 cctgctcttt gtcaaccgtt tatgaagaac atcactggaa acaatcaagt tgcgttaggg
    13381 cataccggaa atatcggtta tgcttctagt ggatggatcg gaaagttttt tggaaacaaa
    13441 gaccgttcta cagaggacat aaagattcct aaaagtttca gctttttgcg agattctact
    13501 gtatcgatca tgctcttgat gtcgatcgta tatgtcgcac ttgctttgtt agctggtcct
    13561 ggatttgttg aaagagaact aagcaatgga gacaacgctg taatctttgc actgatccaa
    13621 gctggaacat tcacagctgg gtttgttatc gtccttcaag gggttcgaat ggtgttggca
    13681 gaaatcgttc cagcattcca agggattgct aaaaaactcg tacccgattc taaaccagca
    13741 ttagatgttc ctatcgtttt tccatatgca cctaatgctg tattaattgg cttttttgtt
    13801 agcttctttg taggaacact atccatgttt gctatgatag gcttgcaaac tacagttatc
    13861 attcctgggg tagtaggaca tttcttctgc ggagctgctt ctggtgtctt cggcaactcg
    13921 actggcggta gaagaggggc ggtattaggt tctgctttca atagcttatt gattagttgg
    13981 ctgccattat ttatccttcc agtattagga gatcttaaaa tcgcttcttc tacatttgcc
    14041 gatactgatt atctagtacc aggacttgta cttgggaaac taggagaata cggtcaaata
    14101 gccttgacag ttggaatcat cgctttcttt ttacttgtcg tattatctag ctttcttttt
    14161 gccggaaaaa gaacaaacat tgtttctaaa gaacagaatt aagcaaactt caaaataaaa
    14221 attgcttgga caatatcaga agaagattta aagtcttctg atatgtcgca agcagttttt
    14281 tgttttatgc atttattttt gaatagcttg ttaagacaag tggctgtttt gctagaagag
    14341 gataactttt accattcatt ttcactgttc cgcctgttct atcagaagta atcacgatct
    14401 tatcttttgt tttttcaagc gaaatctttc catctttgat aggtagcgta caattcagtg
    14461 tttcaaatga atctagatta ggttgtatca taaattcttc ataacctggc tttgttggat
    14521 acaaaccgat aaaatagcgt cctaaaagat agattgggct tgctccccat gcatggcata
    14581 agctttttcc ataaggatct ccatacatac tgtacttttc atttcctgtt tgagtagggt
    14641 cgtattcttc ccagaaagtt accgcatcat gagataacat ccctccccaa taatcaagaa
    14701 tcgtttggta gacgatattc gtctgtccga gtttgcataa agcatcttgt tcatagaatt
    14761 tgaaatatgg agtagtaatc tgtgtgatag tgtcattcaa taagacattc tttaagatcg
    14821 attgctgttg gtctttgtta gcaaaatcga ataaaacagc gaaaatattc gcatgacgcg
    14881 tcacatttct tctcccagat tcatagcaat cgataaaagc gccaagttct tcatcccaaa
    14941 agtagtcaaa taggttttgt ttcaatagtt gatatttttc ttgatagaga tgagtatgat
    15001 aattttcgtt ctctccatat gtttcagcta agatatctcc gcaagtgatc atggaatgat
    15061 aggctttcag taataagact tgttctgcag caagcgttcc ttctttatcc atttcagacc
    15121 aatcaataaa tatccaatct tttgctctcc cataaataaa ccctaattca ttcgtttgct
    15181 ccatcaagta tctcattaat gattgcatct tagggtagac gatgcgtaaa aattcatagt
    15241 ctttcgtcat catatagtgg ttttcgatac caatcaacca caagatcgaa tagtcaacga
    15301 tagtattcat atgctgcttg atttcatttt gtcctcttaa tgctaatatc gttcgtttat
    15361 tgatttcctc gtcaaaaaaa agatattgat tgatcatgaa tgattgatac gcatctccag
    15421 accaaatcca gcgatctcgt ttgataccgt cgataaaaaa caaatcacta cataacttga
    15481 aggcttcttc tgatactgac cagatctgat tcagtaaaga agaatcgctt gaaaaagcag
    15541 cttgtttcga aagagataaa aattcatgaa aaacttgaag ttgaaccatt ccagtagaaa
    15601 cctctggtat aaacaaatag cgaaaagcgc gtttacgagc tttcattttt cttgtcattc
    15661 ctatttgctt atagtagcaa taatcgatat ccatagcttc tacatcggac tctccataac
    15721 atagagtgat agattcgata tggtcatcca gagatattag ttctacacag ccattcactg
    15781 ctcgcccaaa atcgaataag attcctccgc caatctccgt ttcagtgact ggatggacgc
    15841 attctttttg ataatagatt tgatttggat cttttgcgat atcgacataa agcggactcc
    15901 agccagctgg atattcttca ataaaattac ttgctgtcca gccaatatct gattttactt
    15961 cgtctccctc gataaacatc gctggtagcc ctgatgtatg tcctacaaag atacgaattt
    16021 ttgctttacc agctggacaa gtcagccatt ttccacatgg atgtttttgt ccattaattt
    16081 ctacataccc aactccttgg atcgtcactt taaacatgga aggttgatca agaaagtaat
    16141 atctcttaaa cttgacgttc cgatggcaat catccatgta ccagtacgct ggccagtcaa
    16201 aacctcgttc ttcccgttgg aaattttgta gcagtccatg gcggatctca aagtctccag
    16261 gataccaaag ccatctatct ttatgttttt catttgtcat tttacacgac ctccatcaaa
    16321 tttattttgt tgtcaattgt tcttttattt cttcatctac tcttatcact gtctttctat
    16381 tcgtcctgtg tactactaga aggctaataa gcgcgctaat caacagcaag atactgccat
    16441 tgacgaaaac agcaagtgtt ttactcccaa gagaagcaaa agagagaaag agcaaaccat
    16501 atggtgcaat gaaagtacct aaattagcgc caaccaataa gattgaagta ttgaaactct
    16561 ccgcgtactt gggcgcttgg atacttactt ggttgaataa gtaaggaatg aataacgaaa
    16621 aagaaattcc tgttaggatc gcaccaaacc caatcacata caggtttatc acagtggcaa
    16681 tcaatacaaa gccgattccc atcagtagta aagaaagtgt aagcatatag cgatgtgctt
    16741 ttgtctgaag ccagccaaaa caaactccgg cagccatact tgccaattgc atgattgtta
    16801 agatattcgc tgcattagtt tcgtttccta gagaataagt cgtcaggact gtggctaatt
    16861 taatcgatac agcattatat accacgatga ccagcaagag aaaaatacca taactaatca
    16921 ttttaggatt cgttttgatt acttgtcttg ttggggctgt gccatgcgat tgaagttcag
    16981 ttgtttcagg tgtaggaaca aacaaaacaa acaagacaag aataggtaag ctgatggcgt
    17041 agactaaaaa ggtatattgc caaccgaact gtactagctg tcctgcaaca aatgtcatta
    17101 aagctgcacc aaatccttga aatgcacttt gaaaaccaat catagaggct cgttcatctc
    17161 cgtgataaaa ggcagcaatc atgctgactg ccaaagaatt gatcaatcca aagccaatac
    17221 ctagtccagc tcttgaagct aagacaatac tgtaactttg tgagaagagg gggaggatac
    17281 cacaaatcga tgcaatcact aatccgatga tcacagtttg tttttgtccg attttcctag
    17341 caataaaact actaatcaaa acaaaaatag tgaccataaa agagggagca gtcgttaata
    17401 attcaacagc agataaagaa cgcgtggcat aagtagctga catactggga attgcagacg
    17461 tgactgcacc agcagaagtc aaaataagag agactgacag cagagaaaat ttcatcatct
    17521 ttttattcat aagcatgttc ctttcatttt tgtaagcgct tatcaaaaat atagtatgaa
    17581 gtggttattc tctcaatagc ggaatcacag gaagttcatg tcccaaccat agaaagtctt
    17641 tatgaaacca aaaatagctt tgcatataat agcagtacag cgtgaactaa taagcgaaag
    17701 aaaccgctta caaaaaggag ggagtcaatt ggaactagct gaattagaca ttttttttgc
    17761 gtaacgaaga atcaatcgaa actctgcagc gtgaacaagg aatgaatatc aatgatttaa
    17821 acttagctta ccatcttcag acaccgccaa aaatccaccg aggggatcta tttcaacagg
    17881 gaaatattca tatcagtaaa caccgtcgat atgcggaagt tcccgagcat acacatgatt
    17941 ttgttgaaat caattatatg tatagcggta catgtttaca aattattaat ggagaaagga
    18001 tagagatgga agaaagtgac gtggtgatca ttgataaaga agctactcaa agaatcgaat
    18061 acacagggca agaggatatt ttaatcaaca tcttattaaa agaagaaacc ttatctacag
    18121 agatattgaa tcatttggca agcacaaata atctactttc ttcttttttg ataaatgcat
    18181 caaaagaaaa aggggaacat acacaattca ttcattttcg cggcggaaaa acaagtcgtt
    18241 tgacgcattt gattgaaaat atgctgttga tttattttga tacatacagt cataagatcc
    18301 gttcgttaaa tctctataca tcactattac tgatcgaatt gactcattta ttagaacaac
    18361 aaagcttttc atcagcagat ggatgcgcag aaaatgagat gcttagagta ttagattata
    18421 tcgataagca ttttacttct tgtacgttaa aagaaacagc agctcatttt ggctacaatc
    18481 cggtctatct tagtaataaa ttgaaaatgt attttgatgc ttcctttcaa gaactggttc
    18541 taaagaaaaa attcgacttt gcgttggaac tgattcaaga aacgagtttt tcgttggaag
    18601 aaatcgctaa tcgtatcgga tataaacaaa cgacttcttt attcaaatta ttcaagaagt
    18661 attcaacatg tactcccaat gaatggagaa ataaacatta agaagaaaat ctttcactaa
    18721 agcatatgaa ggaaaattgt tttaggtgcg attgctgtct tattatatcg ttgcagcgaa
    18781 tttttttgga tttattttca gttctaaact tttggttgta ttatcagggg atataggtgt
    18841 aaacatttgt catttatctg ttatgcgtat tcaggaattc gtttccatca aaaattataa
    18901 taacaactat tataattaac atgttgataa aatgaataaa caagactata cttttcatga
    18961 aagtaacaaa tagttatgaa aaggagctgt ttattcatga aaaaaacaag tcgtaaagta
    19021 gtaatcgtcg gtacaggttt tgttggaaca agtatcgctt atgcaatgat caaccaagga
    19081 atctctaatg aattagtatt gatcgatgtg aatcaagaaa aagcagaagg ggaagcattg
    19141 gaccttttag atggtatggc ttggggcgat gaaaatgtgg ctgtatggtc aggtggctat
    19201 gaagaatgta aagatgctga tatcgttgtt atcacagctg gtatcaacca aaaacctggt
    19261 caatctcgtt tagatttagt taaaacaaat gcgtcaatca tgcgccaaat cgtaaaagaa
    19321 atcatgggat caggattcga cggtattatc gttgttgctt caaacccagt agatattttg
    19381 acatatatcg cttggaacga atctggtctg ccaacatcac gtgttatcgg tactggaaca
    19441 acattggata caactcgttt ccgtaaagaa atcgcattga aattaaaagt tgacccacgc
    19501 agcgtccacg gctatatctt aggtgaacat ggtgattcag aagttgctgc atggtcacat
    19561 acaactgtcg gtggtaaacc agtgtttgaa attgtagaaa aagaccatcg tattgcccaa
    19621 gatgaattag atgtgattgc tgataaagtc cgtaatgctg cttatgagat cattgatcgt
    19681 aaaaaagcaa cttactatgg tattggtatg agtactgcac gtatcgtaaa agctatcttg
    19741 aataacgaac aagctgtatt gcctgtatct gcttatttaa ctggtgaata cgacgaaaaa
    19801 gatatcttta caggtgtgcc atcaatcgtt gacgaaaatg gtgttcgtga agttgttgaa
    19861 ttatcaatca acgaagaaga aaaagcaatg ttcagcaaat caacaagtgc tttaagagaa
    19921 gtattgaaca ctgtattata agaaacaatg aatatgaact aaaaatccta aaactgctta
    19981 acagctgaaa tagctgttaa gcagtttttt attgatatct aataggtaag caaataaaca
    20041 gaatggttaa aattaatcgg tccgaaaatg tatccgtaca tatgaacata tgaaatttaa
    20101 tctgttgatt acaataagcg aatcgttatc aatgtttatc tgtggttaat taaaaaatcc
    20161 ttccg
//
LOCUS       DAHUWM010000031        20080 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHUWM010000031 DAHUWM010000000
VERSION     DAHUWM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.031"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            43..474
                     /gene="mraZ"
                     /locus_tag="NK788_000789"
     CDS             43..474
                     /gene="mraZ"
                     /locus_tag="NK788_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCH6769135.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            495..1454
                     /gene="rsmH"
                     /locus_tag="NK788_000790"
     CDS             495..1454
                     /gene="rsmH"
                     /locus_tag="NK788_000790"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCH6769136.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1459..1860
                     /gene="ftsL"
                     /locus_tag="NK788_000791"
     CDS             1459..1860
                     /gene="ftsL"
                     /locus_tag="NK788_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCH6769137.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1861..4053
                     /locus_tag="NK788_000792"
     CDS             1861..4053
                     /locus_tag="NK788_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCH6769138.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4079..5041
                     /locus_tag="NK788_000793"
     CDS             4079..5041
                     /locus_tag="NK788_000793"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCH6769139.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5055..6416
                     /locus_tag="NK788_000794"
     CDS             5055..6416
                     /locus_tag="NK788_000794"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCH6769140.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6442..7530
                     /gene="murG"
                     /locus_tag="NK788_000795"
     CDS             6442..7530
                     /gene="murG"
                     /locus_tag="NK788_000795"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCH6769141.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7545..8765
                     /locus_tag="NK788_000796"
     CDS             7545..8765
                     /locus_tag="NK788_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCH6769142.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8921..10246
                     /gene="ftsA"
                     /locus_tag="NK788_000797"
     CDS             8921..10246
                     /gene="ftsA"
                     /locus_tag="NK788_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCH6769143.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10269..11510
                     /gene="ftsZ"
                     /locus_tag="NK788_000798"
     CDS             10269..11510
                     /gene="ftsZ"
                     /locus_tag="NK788_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCH6769144.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11527..12204
                     /locus_tag="NK788_000799"
     CDS             11527..12204
                     /locus_tag="NK788_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCH6769145.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12218..12817
                     /locus_tag="NK788_000800"
     CDS             12218..12817
                     /locus_tag="NK788_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCH6769146.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12832..13119
                     /locus_tag="NK788_000801"
     CDS             12832..13119
                     /locus_tag="NK788_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCH6769147.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13168..13950
                     /locus_tag="NK788_000802"
     CDS             13168..13950
                     /locus_tag="NK788_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCH6769148.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            13971..14678
                     /locus_tag="NK788_000803"
     CDS             13971..14678
                     /locus_tag="NK788_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCH6769149.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            14940..17738
                     /gene="ileS"
                     /locus_tag="NK788_000804"
     CDS             14940..17738
                     /gene="ileS"
                     /locus_tag="NK788_000804"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCH6769150.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17787..19313)
                     /locus_tag="NK788_000805"
     CDS             complement(17787..19313)
                     /locus_tag="NK788_000805"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCH6769151.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19328..19975)
                     /locus_tag="NK788_000806"
     CDS             complement(19328..19975)
                     /locus_tag="NK788_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCH6769152.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6815 a         3491 c         4308 g         5466 t
ORIGIN      
        1 tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat gggtgaattc
       61 caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg tgaagaactt
      121 ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta tccgatgagc
      181 gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa agacgcacgt
      241 acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa acaaggacga
      301 atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg cgtagtcatc
      361 ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt ttctgcagca
      421 gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact ataagaatag
      481 gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg aaagaaacgg
      541 tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc ggcggtgccg
      601 gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat gcgtttgacc
      661 aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta
      721 tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt gagcatgtag
      781 ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta gatgaagcag
      841 aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat cagtcagctc
      901 ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta aagattttct
      961 tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa
     1021 aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa gtcatcccag
     1081 ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct gtgcggattg
     1141 cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc cggttattga
     1201 aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga attgtcaaaa
     1261 gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct gtcatacctg
     1321 acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct tcagaggaag
     1381 agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa aaaataagaa
     1441 tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga atatgattta
     1501 cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc
     1561 attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga aaaaattgct
     1621 gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat gctgcgcaca
     1681 aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag
     1741 caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga acggattaaa
     1801 caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa agtgaagtaa
     1861 atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa tccgatgaac
     1921 aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt ctttttattt
     1981 gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc attagctgaa
     2041 aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg aacgatttat
     2101 gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa agcgatactc
     2161 tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa
     2221 atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact gaatatttta
     2281 gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc
     2341 tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt
     2401 tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc tcattttatt
     2461 ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg tttggagtct
     2521 gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa agataatttt
     2581 caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc
     2641 tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga tcaagtaaat
     2701 gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac cggagaaatc
     2761 ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt gactggaaaa
     2821 gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc aaccatgaaa
     2881 gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga aaccttccaa
     2941 tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg tgaaaaaggt
     3001 gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt catactggaa
     3061 cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg acaaagtact
     3121 cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat tgtcgaccga
     3181 gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat gaaagctttc
     3241 acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa agtggtggat
     3301 ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc tttttcaaaa
     3361 gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga aaactacggt
     3421 agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc
     3481 caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct gtattcgatt
     3541 gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat gaaacaccct
     3601 aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa acgggcaatg
     3661 gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa agtatccgta
     3721 gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa aagtggactg
     3781 caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc caacggtgat
     3841 cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct gatgcctgac
     3901 gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg tattgaagtt
     3961 agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga aaaaatcact
     4021 gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag aaaagagcat
     4081 ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat
     4141 gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg
     4201 accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag tgtttctgat
     4261 cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa ctcctactct
     4321 tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg atgattttat
     4381 aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga ttggacagat
     4441 tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag gtgtattgaa
     4501 tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc tcttctggct
     4561 tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag ttgctggctt
     4621 aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc agtatgatgt
     4681 attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct acaacagaaa
     4741 acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac ttttagcggc
     4801 aatttctata atgctaaacc aagaatggac attgcttttg attggtttga tctatgttat
     4861 ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa aacgaatttt
     4921 taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt ggaagattga
     4981 tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga tcgtttggta
     5041 ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg cttgtcttag
     5101 gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc gcacttgtca
     5161 ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg ctttctttag
     5221 ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa ttttcattga
     5281 tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc caagaaatgg
     5341 gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc ccaattattg
     5401 ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta ctactgaatg
     5461 caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca gcaagtggtg
     5521 tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc ttccaactca
     5581 tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat gaagcacata
     5641 ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt caaaaaaaca
     5701 tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa gaattagcgc
     5761 aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt
     5821 atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata tctgaacttg
     5881 gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta gcaaaattgt
     5941 atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc gtaccacatc
     6001 gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca aaagcaacca
     6061 atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc gtgttattgg
     6121 ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta aaaggaatca
     6181 aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga aaaaaagcag
     6241 gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta gcattagaaa
     6301 acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg gatcaatacc
     6361 caaattttga aactagagga aacagattca tggaagctgt gaaccgatta aaatagaaaa
     6421 agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac agggggacat
     6481 atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa tgctgaattc
     6541 atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac aggaatgcct
     6601 ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa catcaagacg
     6661 gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca
     6721 gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa
     6781 atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac caataaattt
     6841 ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc cttttttcct
     6901 gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga tactaaaaaa
     6961 tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt gatttttggc
     7021 ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag tgaatatatt
     7081 ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga gatcctaaat
     7141 aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa tatggcagag
     7201 gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat cgcagaattc
     7261 acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa tgatcatcag
     7321 actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc agataatgaa
     7381 ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga agaattgcaa
     7441 aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa acgtctttat
     7501 gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt aagaaaaaag
     7561 aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg
     7621 ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa aaagaagaca
     7681 taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa gaggaacagc
     7741 aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag caaaagaaaa
     7801 acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa agtcaacggc
     7861 acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata ccgctgattt
     7921 ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta tcaggcaata
     7981 agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga gaaaatgttt
     8041 ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca cagccaagaa
     8101 tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat gtaacagaat
     8161 ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa
     8221 cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg gaagagttca
     8281 aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca tcagaattac
     8341 aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat ctgatccaat
     8401 tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg aaccaaatga
     8461 aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac atggaagttg
     8521 gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc caatcggaag
     8581 aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa
     8641 cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt
     8701 caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt ttaaatgaaa
     8761 attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta atttatggta
     8821 gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac gctagttgtt
     8881 cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa caggaatgta
     8941 tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat atatcgacag
     9001 ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc gaggcattat
     9061 cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag cagaagaaaa
     9121 agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga
     9181 aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg atgaagatgt
     9241 cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc
     9301 aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat
     9361 gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa cgatcatcca
     9421 taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg tgattactcc
     9481 tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta cgatcgtcat
     9541 tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat tgaagtttac
     9601 caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg tattaaatac
     9661 gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc cagaacggac
     9721 atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag tgaaagtcga
     9781 tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca acaaagcaaa
     9841 agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg
     9901 agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa
     9961 attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg taataagtat
    10021 cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg
    10081 tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca catatgagag
    10141 ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt ccggagaaaa
    10201 tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca tcctgggagg
    10261 aataagatat ggaattctca attgataata atatcaatga cggcgcagta atcaaagtca
    10321 tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa aacgttaaag
    10381 gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca aaagctgaaa
    10441 cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct cagccagaag
    10501 ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta gacggtgcag
    10561 atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg
    10621 ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca
    10681 cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa
    10741 atgtggatac attattaatt atctcaaata accgtttatt agaagttgta gataaaaaga
    10801 caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt gtacaaggta
    10861 tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac gtgaaaacag
    10921 tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg
    10981 tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct attgatggag
    11041 ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc gaagcacaag
    11101 atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat
    11161 ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg
    11221 aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg caatcacctg
    11281 ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca
    11341 gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt gatgattcca
    11401 attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa acaaaatctc
    11461 atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa aggagaggaa
    11521 gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct
    11581 cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc tgttgaatcc
    11641 gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa ccgagtagat
    11701 aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg gcatttaatt
    11761 ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta ctttcatgcg
    11821 ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga attatcctgt
    11881 tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc agatgagctt
    11941 ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg gttgatgact
    12001 atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac gctgaaagaa
    12061 ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga attaagtatg
    12121 gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat ccgtgtagga
    12181 acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa acagaggagc
    12241 aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag attatcctga
    12301 aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc aatcaactca
    12361 ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac
    12421 gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc aatcacctac
    12481 aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta aagcaaatct
    12541 tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg cgattgctaa
    12601 acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag aaaaacaagc
    12661 gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag atatcaaacg
    12721 tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca gttcaaccgc
    12781 tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac gatttatact
    12841 attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat tatcttgatg
    12901 gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg acgattatta
    12961 gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag gattggaccc
    13021 ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca aggtttagca
    13081 cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca aaatctttat
    13141 tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca ctttcgggca
    13201 gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca aggacaatat
    13261 gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga aacactgatt
    13321 cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc agaaagaaaa
    13381 agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga aacagcattg
    13441 tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat ccttggaact
    13501 ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga cggtgatcgc
    13561 tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt aacaaagatt
    13621 ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat gccgaaagat
    13681 agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt gatctctgag
    13741 atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa agtaaaagtg
    13801 aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat tatttcagtc
    13861 agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa agaaaagtac
    13921 agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc atggctttaa
    13981 ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat aatcaagacg
    14041 aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact caaagaaatc
    14101 gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc aatgaattaa
    14161 aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa
    14221 gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca gatgagttag
    14281 ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa aaagcaagaa
    14341 aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact gaagatctta
    14401 agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct caattagaac
    14461 aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac gtgactgaca
    14521 gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat gatactgatg
    14581 taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca caggctattg
    14641 atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt
    14701 cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga aagatcctct
    14761 acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc gttattggtc
    14821 acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg catgttcgtc
    14881 ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa
    14941 tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc
    15001 caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac caaaaaagac
    15061 aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca tatgccaatg
    15121 gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc attcgttcaa
    15181 aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat ggattgccga
    15241 ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg gcagaatact
    15301 tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca gatttcaaac
    15361 gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct tcttatgaag
    15421 ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga
    15481 aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa attgaatata
    15541 aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc aaagatattc
    15601 ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg ccagcaaacc
    15661 aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat ggccgtaagt
    15721 ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa
    15781 cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt catccattct
    15841 atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct ggtactggat
    15901 tagtccatac tgctccagga catggggaag atgactatat cgctggtagc aaatataaac
    15961 tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca cctggatttg
    16021 aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa gaaaaaggag
    16081 ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg acgaaaaagc
    16141 ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc cgtcagaata
    16201 ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca cgtttgtata
    16261 atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac
    16321 ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca atcgaacatg
    16381 tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac
    16441 ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag
    16501 atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta cgtcagcgac
    16561 cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat cgtggctggt
    16621 tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac aaatcagtta
    16681 tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct ttaggaaaca
    16741 tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc ttatgggtat
    16801 ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt caagtggcag
    16861 aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca gaagatttcg
    16921 atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa tatatgaccg
    16981 ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa tataacttca
    17041 tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca ttctacctgg
    17101 attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt cgttgcatgc
    17161 aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct atcattccgc
    17221 atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt cagttaagcg
    17281 agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc tggactgctt
    17341 tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac agcaaattga
    17401 ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt cgtgaaatgt
    17461 taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac tactttgaag
    17521 ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat gttgctatta
    17581 cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag
    17641 gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct
    17701 atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat
    17761 gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc acgataccag
    17821 ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa agctgcttta
    17881 ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc ccaagctttt
    17941 cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga gaagttcgtt
    18001 ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct attttcaacc
    18061 atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc ttgccctact
    18121 tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat cgttagaaca
    18181 ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa tacattaaca
    18241 ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg tttgcctgta
    18301 cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt gcctgcgacg
    18361 aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc cttgaattgt
    18421 acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa cacttcttct
    18481 tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat ttctttttcg
    18541 gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat atgattttgc
    18601 accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc tacaccgagg
    18661 ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg agcctcaaaa
    18721 atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc taaataatga
    18781 tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt caattcaaaa
    18841 gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa gccatttttt
    18901 gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg aggtgccata
    18961 gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg gtcagacaat
    19021 tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata gtaaaaatgg
    19081 cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt ttctttgaca
    19141 acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac agcaaaatgc
    19201 tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt acgctgggct
    19261 aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga catgtggata
    19321 cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg acggggattt
    19381 tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca acgatctcat
    19441 attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct ttttcatcaa
    19501 gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt tgtcggcgtt
    19561 cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa tgttcaggat
    19621 gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt tccaatactt
    19681 cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa aaaacttccc
    19741 ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca gtcaattgta
    19801 caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa atcatttcac
    19861 tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg actttcgttg
    19921 tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt gtcatattcg
    19981 atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa cgttaattga
    20041 ctatttttct aaataaaaaa caaaggattt ttagacgttt
//
LOCUS       DAHUWM010000032        19489 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHUWM010000032 DAHUWM010000000
VERSION     DAHUWM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.032"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(407..766)
                     /locus_tag="NK788_000807"
     CDS             complement(407..766)
                     /locus_tag="NK788_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCH6769153.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="NK788_000808"
     CDS             complement(796..1137)
                     /locus_tag="NK788_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769154.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="NK788_000809"
     CDS             complement(1134..1808)
                     /locus_tag="NK788_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCH6769155.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="NK788_000810"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="NK788_000810"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCH6769156.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="NK788_000811"
     CDS             complement(4322..5512)
                     /locus_tag="NK788_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6769157.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="NK788_000812"
     CDS             complement(5533..6060)
                     /locus_tag="NK788_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCH6769158.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="NK788_000813"
     CDS             complement(6107..8896)
                     /locus_tag="NK788_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCH6769159.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="NK788_000814"
     CDS             complement(9043..9243)
                     /locus_tag="NK788_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6769160.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="NK788_000815"
     CDS             complement(9696..10016)
                     /locus_tag="NK788_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769161.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="NK788_000816"
     CDS             complement(10519..11637)
                     /locus_tag="NK788_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769162.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="NK788_000817"
     CDS             12108..13415
                     /locus_tag="NK788_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCH6769163.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="NK788_000818"
     CDS             13536..14735
                     /locus_tag="NK788_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCH6769164.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="NK788_000819"
     CDS             complement(14762..16030)
                     /locus_tag="NK788_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCH6769165.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="NK788_000820"
     CDS             complement(16200..16841)
                     /locus_tag="NK788_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCH6769166.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="NK788_000821"
     CDS             complement(17250..17753)
                     /locus_tag="NK788_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6769167.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="NK788_000822"
     CDS             complement(17876..18640)
                     /locus_tag="NK788_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCH6769168.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="NK788_000823"
     CDS             18748..19023
                     /locus_tag="NK788_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCH6769169.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5587 a         4145 c         3459 g         6298 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaat
//
LOCUS       DAHUWM010000033        19369 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHUWM010000033 DAHUWM010000000
VERSION     DAHUWM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.033"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(413..1366)
                     /locus_tag="NK788_000824"
     CDS             complement(413..1366)
                     /locus_tag="NK788_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6769170.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1389..2144)
                     /locus_tag="NK788_000825"
     CDS             complement(1389..2144)
                     /locus_tag="NK788_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6769171.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2141..3100)
                     /locus_tag="NK788_000826"
     CDS             complement(2141..3100)
                     /locus_tag="NK788_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCH6769172.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3093..4043)
                     /locus_tag="NK788_000827"
     CDS             complement(3093..4043)
                     /locus_tag="NK788_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCH6769173.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4193..4645
                     /locus_tag="NK788_000828"
     CDS             4193..4645
                     /locus_tag="NK788_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCH6769174.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4734..7001)
                     /gene="gshAB"
                     /locus_tag="NK788_000829"
     CDS             complement(4734..7001)
                     /gene="gshAB"
                     /locus_tag="NK788_000829"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCH6769175.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7188..8186)
                     /gene="ftsY"
                     /locus_tag="NK788_000830"
     CDS             complement(7188..8186)
                     /gene="ftsY"
                     /locus_tag="NK788_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCH6769176.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8197..9006)
                     /locus_tag="NK788_000831"
     CDS             complement(8197..9006)
                     /locus_tag="NK788_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6769177.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9015..12596)
                     /gene="smc"
                     /locus_tag="NK788_000832"
     CDS             complement(9015..12596)
                     /gene="smc"
                     /locus_tag="NK788_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCH6769178.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12616..13302)
                     /locus_tag="NK788_000833"
     CDS             complement(12616..13302)
                     /locus_tag="NK788_000833"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCH6769179.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13650..15431)
                     /locus_tag="NK788_000834"
     CDS             complement(13650..15431)
                     /locus_tag="NK788_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6769180.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15457..16371)
                     /locus_tag="NK788_000835"
     CDS             complement(15457..16371)
                     /locus_tag="NK788_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769181.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16398..17360)
                     /locus_tag="NK788_000836"
     CDS             complement(16398..17360)
                     /locus_tag="NK788_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769182.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17360..18304)
                     /locus_tag="NK788_000837"
     CDS             complement(17360..18304)
                     /locus_tag="NK788_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769183.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18305..19309)
                     /locus_tag="NK788_000838"
     CDS             complement(18305..19309)
                     /locus_tag="NK788_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769184.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5333 a         4195 c         3432 g         6409 t
ORIGIN      
        1 gacagaaaat agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga
       61 tggaacatcg tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat
      121 ctaagtttcg ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa
      181 tggatcacac tattttttgc cactccccat tctagaatca acccgtatga tgtagataat
      241 gaattcacgt tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata
      301 cgattttacg gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg
      361 taagaaacat tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa
      421 atgcttgatt cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat
      481 accagacatc tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta
      541 ccagttcatt ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt
      601 ctactacaaa taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac
      661 cgtgagtgga tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga
      721 aaccgaatct agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta
      781 gtgctttttt gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt
      841 cctctaattg agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat
      901 tttgctggaa agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa
      961 tttgacttag atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct
     1021 gaagctgatt aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt
     1081 tataggatga aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa
     1141 caccaagagc atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat
     1201 tatttggcac ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt
     1261 gtgtgctgct tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta
     1321 aaccaaatga tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa
     1381 tgttagtttt agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc
     1441 atttcgtata attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta
     1501 aataactgtc catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata
     1561 tcatgaagaa caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc
     1621 atgatttgaa ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa
     1681 tccgtgtctt gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga
     1741 gtgtcaatcg attgatccgc tatttcttct aacccaaggt gttccagtga ttcctgaacg
     1801 atttcctcgt cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact
     1861 aattctttca cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt
     1921 ttggctaatt cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct
     1981 gtatcttttg gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt
     2041 ggaccgataa aagcagtcaa tttcttttca ggaatcgtta actgtacatc tttaaccact
     2101 gattttccat ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt
     2161 cgcgaatcaa ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg
     2221 ttgtattcca gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag
     2281 ctaccagaaa gcttccgata aatagctgac catgacgata agtagaaaag acacgataac
     2341 tgatatttgc gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc
     2401 ctgtcaaaag actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga
     2461 ggtttactgc ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata
     2521 acaggtacca gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat
     2581 tgccaaagct tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga
     2641 cttgtaaaaa ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta
     2701 ataaaaaaag attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc
     2761 cgaccatcag cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca
     2821 ctcctccgat aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca
     2881 atactgaagg agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc
     2941 tgacagctag cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa
     3001 aatgccagtt tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa
     3061 caagcatacc aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga
     3121 agataaagga agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa
     3181 ggaaaaatca acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa
     3241 gcagttacgc caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga
     3301 atgatcactc cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat
     3361 gatactaagc caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca
     3421 aacgattcgc ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac
     3481 aatggaacaa cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct
     3541 tgaagccatg aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact
     3601 gaaccaatga tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg
     3661 aggaacaatc gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct
     3721 gtcaaggatc ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct
     3781 aaacgcgcac tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa
     3841 tgttgcatga tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt
     3901 cgtggcaaac gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct
     3961 gacaattgta cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat
     4021 gctactagtc gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct
     4081 tattttaagt gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg
     4141 ggaatatgct ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca
     4201 agatgatgta aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga
     4261 agaacagcat aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc
     4321 tgaaatgatc caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga
     4381 tgcttccata ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct
     4441 agcaacgaaa aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga
     4501 ggaattcgga ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga
     4561 aaaaaaatac ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa
     4621 aagcttctgg gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa
     4681 agcttttgat cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat
     4741 acttcaggat ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa
     4801 ggataaacat gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt
     4861 ttatcagttg taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta
     4921 gctcctagtg cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca
     4981 gtcatatcga tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt
     5041 tgattggctt ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct
     5101 aattcaccta attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca
     5161 ctattttttt cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca
     5221 gggactcgca acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt
     5281 cccggcataa attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct
     5341 gcttgataat cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg
     5401 gttgatttag gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct
     5461 tcttccatgg atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact
     5521 tttttcgtta ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc
     5581 atgtttgcat ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc
     5641 gactcatcta atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga
     5701 gtagaaagtt ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta
     5761 taatattgat gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga
     5821 tagagtttcg ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact
     5881 aaataaagag aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt
     5941 tctctacctt cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta
     6001 tttcgagtcg taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac
     6061 atgtacatca ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca
     6121 taaggattca aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct
     6181 gcgactttct ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt
     6241 ttcccttctt cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa
     6301 actttcacat ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt
     6361 tcttgcggat gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat
     6421 aaataagtga tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct
     6481 gttttgaatt cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac
     6541 tcatcgccaa attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat
     6601 tcttttgcta aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg
     6661 atctcttcgt ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct
     6721 ttcggtatag aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga
     6781 atagaatcgg taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata
     6841 taggggtgat aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct
     6901 gcaagatcaa cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg
     6961 cgcgcattga catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat
     7021 atgaatctaa ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt
     7081 acaaataagt ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa
     7141 acaaggagct gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt
     7201 agtaatcctt tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg
     7261 attccttcac caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt
     7321 acgataccgc cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt
     7381 gtttctttga attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca
     7441 agaagtgttt cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct
     7501 aattcgttca tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca
     7561 tctgcctgct ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct
     7621 cctgcattgc cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg
     7681 attgctccag cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg
     7741 aactgatggg caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac
     7801 aaaataactg ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc
     7861 gcttcataga gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc
     7921 gcatttctga gtttcacttc ttgacgtaat gcttcggtaa ttcgtatcgc tgtttcaaaa
     7981 cctacatctg caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt
     8041 cgaaaattgg caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa
     8101 cccttgtcgt acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca
     8161 cgcttgattt tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat
     8221 cactttggct acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac
     8281 gaaaggcaca gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc
     8341 attttcctca tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa
     8401 taaagaaata ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct
     8461 tgttttgatg atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc
     8521 taattcttct tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc
     8581 gcttatatcc gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt
     8641 ccttggggca ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc
     8701 caattgctga gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc
     8761 catgatttca ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc
     8821 accttcatct accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc
     8881 tgtacataga acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc
     8941 atctgaaatc agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt
     9001 gatcattttc gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc
     9061 atccgtacag aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca
     9121 tctgctgcga ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg
     9181 aaatcgctca agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc
     9241 tcatctaaga tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg
     9301 gctgtaagtg ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga
     9361 ggttgtactt caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat
     9421 tcagctttac cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct
     9481 tcaaatactt ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac
     9541 aattggtttt tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca
     9601 ctgacttgtt cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt
     9661 tcttttaagg atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct
     9721 tgggaagctt tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct
     9781 aaaatcattt cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct
     9841 ttttgctcac gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt
     9901 ctatctcgct gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat
     9961 aattcgttaa tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct
    10021 gtcaatgtcg ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg
    10081 actcgggcac cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga
    10141 tctgcttgtt cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg
    10201 ctttcttgac ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct
    10261 gtttgtttgt ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt
    10321 tcacgtgtct caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg
    10381 atgttttgta gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta
    10441 cgcaaaactt cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt
    10501 ttttcttgtg cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat
    10561 tcttgatttt gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg
    10621 gatcctccgg cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc
    10681 gtttgcgcga ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg
    10741 ttttgaacca cagtttggat ttgatcggga taactgactt gttcactggc aatccctata
    10801 aacccttcta cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt
    10861 gtcgtcagtg gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct
    10921 tgccgagcat ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgtt
    10981 tcgatagcta acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata
    11041 cccgaaagct gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag
    11101 ttttcttgga tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt
    11161 aattgataca ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt
    11221 tggatcattt ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt
    11281 tgtgtcaatt cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc
    11341 ttggctaacg tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc
    11401 agttcgtttc ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct
    11461 gccaataatt ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga
    11521 tttgcttcag ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct
    11581 tgaagctttt caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct
    11641 tgcgagcttt tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc
    11701 tgcttcagtt tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg
    11761 gcttgcgcat tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca
    11821 gatagtttgc ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct
    11881 ttttttgctg tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct
    11941 tttagccgta aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct
    12001 tccaattcgt ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc
    12061 tgttctgctt tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc
    12121 ccacgacggt cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt
    12181 gagaacgatt cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg
    12241 cagctttgtt tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca
    12301 ctgatctcat tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact
    12361 tctgcaatat tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt
    12421 ccgcctcgca gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata
    12481 ttgctttttc cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg
    12541 actgtcttgt ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct
    12601 ttccctcact tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa
    12661 ttttttcgat ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca
    12721 aaatactcgt tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc
    12781 acctgatttt tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc
    12841 acctgctttg actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc
    12901 ctggtcaaga tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg
    12961 cgtacgtcct cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca
    13021 ttctttggca aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa
    13081 ttttccttct ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa
    13141 aacggcatct cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc
    13201 attcacatag gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat
    13261 gttatagttt tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat
    13321 ggatattttt tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg
    13381 tcaaaaggta agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg
    13441 tttagtttcg agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa
    13501 tttgacttaa taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc
    13561 agcgccttga cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga
    13621 catcgccctt tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca
    13681 tttgcccatg ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact
    13741 ggcaatactt cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt
    13801 tgccattttt gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata
    13861 tcgttgatca attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt
    13921 ggtccccata gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca
    13981 tcaatttcag gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga
    14041 cctgttgtgt attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc
    14101 aatggttgcg ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca
    14161 tctttgtctt ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt
    14221 tttttcgctt tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg
    14281 aatactggag gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct
    14341 actgcattat tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt
    14401 gaatctggat tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct
    14461 agataagtat aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag
    14521 gtatcgtaag tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct
    14581 tcagttgcac ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca
    14641 ttccagtagt gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg
    14701 taataagggc caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg
    14761 attgctacac cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat
    14821 tgaagcatgc ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt
    14881 ttgattccgg agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta
    14941 cggaaagtgt cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg
    15001 aaaatcacat cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact
    15061 ttgattgtcg cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg
    15121 attctgaaat cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca
    15181 tcatatgaat cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg
    15241 accgcagctt ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga
    15301 aaagttaatt cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta
    15361 cctccgcccc cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc
    15421 gttttcttca ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc
    15481 tgcagaacgc ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag
    15541 gattgatgcg ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata
    15601 accgatcaat gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa
    15661 agttgtctcg ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga
    15721 acttagattc ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt
    15781 tgaggcactg acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc
    15841 tttagctacc caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga
    15901 aataatcgat acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac
    15961 gatccgcatc aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc
    16021 aaccccgatg atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc
    16081 acctatgatc aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt
    16141 ttgtccttgt gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat
    16201 cactttgtct tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag
    16261 caaacctaat gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat
    16321 ccccatagga ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt
    16381 ctcctttctg attggtttta ttgaatccga atacgcgggt caacgatact catgatgata
    16441 tctgaaagta atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc
    16501 acggtatagt cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca
    16561 aaaatctttt cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga
    16621 gaagccatcg gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt
    16681 acccctttgg aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt
    16741 cgcagatatt ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga
    16801 atatatttca aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt
    16861 gatccacttg ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg
    16921 aagataaata aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag
    16981 attgaatttt gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg
    17041 acaactgtca agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca
    17101 acagagcgtt tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa
    17161 cggaagtact ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc
    17221 actgccggat cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg
    17281 gctaataaga agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt
    17341 cgcagaatcg ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag
    17401 ttgtgatcgt acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt
    17461 ggatatactc ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat
    17521 cgatttgcgg aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa
    17581 tgtcttctct tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc
    17641 acatgtgttt tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt
    17701 gctggattcg tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata
    17761 caggttcatc ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac
    17821 gttgacgttg tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc
    17881 caacgatatc tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa
    17941 aatttcggat gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca
    18001 ttgaatcttg gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc
    18061 gcttcgttac atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca
    18121 caacggcttt tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct
    18181 ttcctttttc gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat
    18241 tgaagaatcc actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg
    18301 tcatctacac ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta
    18361 ccaagtggtt cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta
    18421 tccatggaat acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa
    18481 cgacaccttc gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca
    18541 gtaaagagcg tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt
    18601 caacgaactg tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta
    18661 agtcatgagt gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat
    18721 caagaatctg tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga
    18781 ttggaggttt gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata
    18841 gttcatgggg atattggcgt ccaactctcg caggattagg aatccctact tgttctagca
    18901 attctagtgc acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt
    18961 ctttgatctg gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa
    19021 tcattccgat atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat
    19081 tcagatcgtt atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca
    19141 taatcgtagt agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct
    19201 cgtttttgtc taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc
    19261 ggaatcctgt atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc
    19321 tctctgcttc ctaaatggaa gtttttctta ataaaaaagg acattacac
//
LOCUS       DAHUWM010000034        18980 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHUWM010000034 DAHUWM010000000
VERSION     DAHUWM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.034"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..1561)
                     /locus_tag="NK788_000839"
     CDS             complement(<1..1561)
                     /locus_tag="NK788_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCH6769185.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKD"
     gene            complement(1565..1846)
                     /locus_tag="NK788_000840"
     CDS             complement(1565..1846)
                     /locus_tag="NK788_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCH6769186.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1843..2766)
                     /locus_tag="NK788_000841"
     CDS             complement(1843..2766)
                     /locus_tag="NK788_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCH6769187.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2763..3641)
                     /locus_tag="NK788_000842"
     CDS             complement(2763..3641)
                     /locus_tag="NK788_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCH6769188.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3628..4965)
                     /locus_tag="NK788_000843"
     CDS             complement(3628..4965)
                     /locus_tag="NK788_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCH6769189.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(4968..6071)
                     /locus_tag="NK788_000844"
     CDS             complement(4968..6071)
                     /locus_tag="NK788_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCH6769190.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6145..7653)
                     /locus_tag="NK788_000845"
     CDS             complement(6145..7653)
                     /locus_tag="NK788_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6769191.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7619..8311)
                     /locus_tag="NK788_000846"
     CDS             complement(7619..8311)
                     /locus_tag="NK788_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCH6769192.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8333..9307)
                     /locus_tag="NK788_000847"
     CDS             complement(8333..9307)
                     /locus_tag="NK788_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769193.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9607..10479
                     /locus_tag="NK788_000848"
     CDS             9607..10479
                     /locus_tag="NK788_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769194.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11061..13259)
                     /locus_tag="NK788_000849"
     CDS             complement(11061..13259)
                     /locus_tag="NK788_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769195.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13491..14483
                     /locus_tag="NK788_000850"
     CDS             13491..14483
                     /locus_tag="NK788_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6769196.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14543..15964
                     /locus_tag="NK788_000851"
     CDS             14543..15964
                     /locus_tag="NK788_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCH6769197.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15957..17456
                     /locus_tag="NK788_000852"
     CDS             15957..17456
                     /locus_tag="NK788_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCH6769198.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17495..18889
                     /gene="uxaC"
                     /locus_tag="NK788_000853"
     CDS             17495..18889
                     /gene="uxaC"
                     /locus_tag="NK788_000853"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCH6769199.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5691 a         3873 c         3359 g         6057 t
ORIGIN      
        1 gatcttttcc atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta
       61 gatcatgcac tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt
      121 ttttttgcgg acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg
      181 ctgtctcttt ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc
      241 aatcttctat ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt
      301 cctcattcca agtaacataa caataaatat aattggattc atttaagtac agtaccagtc
      361 cagccatctg attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa
      421 aatccgtttg ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg
      481 aaatcagacg taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa
      541 tgcgtctggc attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat
      601 ctttttcttg ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct
      661 ggctatcttt aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt
      721 ctcttcccaa aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc
      781 ttcctgacgg ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt
      841 tgtcactagc tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct
      901 ttgctcggca aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat
      961 aataataccc gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg
     1021 caaatatttt atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata
     1081 cgataccgct ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc
     1141 atttttttcc cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat
     1201 aaattggttc ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat
     1261 ctttaaacgg acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag
     1321 cataactgat ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt
     1381 gagtggtcaa aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc
     1441 ggattcctgg aagccattca aaagaggaga caataatgta ataccgctct ttcactctta
     1501 atatattagg atctggattg aatcctctta aaataggatt tttcactttg atagtctgca
     1561 tgattcaggc tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa
     1621 aagcaagtga aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa
     1681 atgtattttc taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa
     1741 actctgacgc agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata
     1801 gactgccacc gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg
     1861 cttttctaaa tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc
     1921 agagaatttg acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg
     1981 aacatgccca cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct
     2041 tcctaaatag acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata
     2101 ccctgttttt tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc
     2161 taagctatgg atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac
     2221 cgttccttca atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc
     2281 cggtgtacaa aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt
     2341 atcctgataa ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa
     2401 agcaacagcc tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc
     2461 taatacgaca ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct
     2521 accgtcaaga tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc
     2581 cacaagttta aaattggata atctgctttc tatatattca tcatatcttc cttcttttac
     2641 tatcaagacc tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat
     2701 agtttcaaaa tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat
     2761 cctcataaat gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata
     2821 tgaccgcctg ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt
     2881 ttgtccagcc cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt
     2941 ccaactaagc taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa
     3001 gctgtcaata gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc
     3061 aaaagtgttt cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc
     3121 aatgctgtca cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt
     3181 tgagtatctg tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata
     3241 cgcattcccc ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa
     3301 atacctccgc gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct
     3361 gtcagtactt ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt
     3421 ttcccgactg aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct
     3481 tctttttctg gataagcata gagggctggt actgtttcca attcatttaa gtacaattga
     3541 tagtattcaa gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta
     3601 tgtctgctag atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac
     3661 aaccagttca tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt
     3721 agtattttct aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga
     3781 aaccttaccc gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat
     3841 catagcagga acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt
     3901 gatttcttgt atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt
     3961 ccttgccgtg atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg
     4021 ataagccttt ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag
     4081 attcagtata aatggacaca tcacattatc catgactata ttggatactc gaatatcttc
     4141 aacgatcccg cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa
     4201 gatacaattt gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac
     4261 accgccatgt ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct
     4321 ttcgtacgta tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg
     4381 gcaattacta atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga
     4441 atccgctggg ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt
     4501 gatcgtccaa cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt
     4561 atggaaactc atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt
     4621 atgccaccat ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc
     4681 tacattttga gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga
     4741 atgaacaaca ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa
     4801 atgaagttcg acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac
     4861 aactacggtc ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt
     4921 attcaattca tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc
     4981 acttcaatca atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt
     5041 tcactgatgt agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact
     5101 tgacagtttt tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt
     5161 aatccttgat gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa
     5221 cgtattcctt tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt
     5281 cctttccgtt ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat
     5341 ggaagtaatg cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct
     5401 tcatgaagta attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc
     5461 caaaaatgat gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc
     5521 catgcatgaa tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac
     5581 tgtctcaaaa catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa
     5641 aaaggagaac ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc
     5701 caaaaagctc cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct
     5761 gcttttttat atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta
     5821 tttacatggt cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc
     5881 acgaaatagt cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc
     5941 caaagtaatt ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac
     6001 cacttttctt catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac
     6061 cagttttcca ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag
     6121 aagtatttgt cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc
     6181 ttgtgtgatt cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc
     6241 ttcgtcaagc tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc
     6301 tgatcctact tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa
     6361 ttcttcattt tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa
     6421 agagaacgtc acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag
     6481 atccatatcg ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat
     6541 accgccagtc atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa
     6601 tttcttcaat tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat
     6661 accatttcct tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac
     6721 aggcactaat tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc
     6781 caccattcca gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt
     6841 ttgggtaact gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt
     6901 caaagctttc atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac
     6961 agcccagcca tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat
     7021 atcactaggc gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc
     7081 gttttctgta aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag
     7141 tcctagatta tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg
     7201 tatcggtacg ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat
     7261 ttgacttaaa tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt
     7321 taacgcattg cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc
     7381 tagttcatca gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca
     7441 gttgaatgtc agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg
     7501 cggtgaagca tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc
     7561 tgaagaactg ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact
     7621 aagacctaat tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa
     7681 tatttttgtt ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata
     7741 tagaatttgc tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa
     7801 cacctggcat tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta
     7861 atgagtaatg aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc
     7921 ctttccaatc ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga
     7981 acgcagcaat atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa
     8041 gtaaaacaat cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt
     8101 ctttaaaata tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt
     8161 ttctaatcac tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg
     8221 cgatgactaa accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc
     8281 ttccgtttat tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa
     8341 cgccgtcttc tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta
     8401 cgaccccttt aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag
     8461 ctgtataaat ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca
     8521 agtacatatg ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa
     8581 tgattactgg acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc
     8641 catccatttc tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga
     8701 tgatcgtaga ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc
     8761 attcagggga tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca
     8821 atacccctcc gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca
     8881 tgaagtgagg gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt
     8941 catttaatag taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga
     9001 acaagatcaa cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga
     9061 aatgtttgaa accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa
     9121 aagcaataac gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg
     9181 gcagaatcat cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc
     9241 ttctgagctt ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac
     9301 tttccataag cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata
     9361 actatctgaa ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg
     9421 atttttgtgc tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa
     9481 accttctatt tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc
     9541 accgatgata aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct
     9601 atctatatga aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg
     9661 tttctaattt tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct
     9721 tttgcttcta cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct
     9781 ttagacgcgt atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg
     9841 attggtgtta caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac
     9901 ggtctttcca gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct
     9961 atgttgttca gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc
    10021 aattcttact gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg
    10081 agaaactttt tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc
    10141 acagattttg gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact
    10201 atctcgcttt tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc
    10261 aacgattcat ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt
    10321 gggatgcaag cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg
    10381 gctgttatcg ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat
    10441 tttgtcaaag gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg
    10501 gtagttgtgt cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc
    10561 ttaaattttt gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta
    10621 tcagaggttg tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg
    10681 cgacgagtaa cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca
    10741 caaaattatg ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga
    10801 actgtgacaa gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc
    10861 cataaataca aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga
    10921 aaaacagcct cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc
    10981 agcagcaaaa catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac
    11041 aagaaaaaca gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat
    11101 tccttctttt taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca
    11161 atctctttta cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga
    11221 ttttgaacaa aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag
    11281 ttagggttat aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct
    11341 gtgtagacga tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct
    11401 tgagagcatt ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg
    11461 ggatgataat tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa
    11521 tctactccca ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact
    11581 tcccgactca aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa
    11641 ttcttcactt cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc
    11701 ggatatctaa taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt
    11761 gaacttggac cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc
    11821 ttgctttcat tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag
    11881 tattctttta cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct
    11941 gcatcatctt tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc
    12001 agcattcgat tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc
    12061 aaatcacgct ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa
    12121 tagcagtcat tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt
    12181 tctactcgat ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta
    12241 actaagtgtg cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc
    12301 aaatctagtt ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga
    12361 gaaatcggac gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc
    12421 agtgttaaag acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca
    12481 ctagagtcaa cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac
    12541 gttttatctt tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat
    12601 acagttggtt gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt
    12661 tcaatagaaa gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct
    12721 atcgtagcaa ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt
    12781 tcgattcctt tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt
    12841 tgatactttt caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat
    12901 ttaccagcca aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa
    12961 tttagttctt tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg
    13021 aatatgtccg agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta
    13081 attttccatt caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa
    13141 ctcgattcga tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt
    13201 acggctccta ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt
    13261 ttcttcctct cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat
    13321 ctaattaaat gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt
    13381 aatttttaac gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt
    13441 aatatgatga atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga
    13501 tcaaagatat cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata
    13561 acagcagttt aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga
    13621 attattcgcc aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac
    13681 tatttttctc aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag
    13741 gcatccattc agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg
    13801 atttaacaga aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg
    13861 atgatcagcc tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc
    13921 gtcacatcaa tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc
    13981 aggcagtaga ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga
    14041 aaaattttcg ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga
    14101 gcatccctgt caatctagcc tattttttaa caggagatta cagcttagaa agcggcctcc
    14161 aaaacatgga atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg
    14221 acgacatagc gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga
    14281 acatttctct gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta
    14341 ccaccgtacg taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt
    14401 tgatcgaagg caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc
    14461 gtgagtcgat caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc
    14521 ataacacaga aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga
    14581 agcacctgta aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg
    14641 gcagattcag cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc
    14701 tttagaacaa ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt
    14761 acaaggactc agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca
    14821 aatgatcaat ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt
    14881 ggcctatatc gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt
    14941 aacagatact cccccacaga gcttcccggg caaactgaca gcctttttat accgacgttt
    15001 caaattaaac aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga
    15061 acgtctaaaa gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt
    15121 tgagtggtta gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg
    15181 ttatcctcgc gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat
    15241 ggtcaaagca gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca
    15301 actaccattc aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg
    15361 tgaacgtaag gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt
    15421 ggcggaattg gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga
    15481 tcagttattc aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat
    15541 ttatgctgac caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc
    15601 gatttcatta aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta
    15661 tatcgaagaa caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct
    15721 tatgtatcga ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac
    15781 tgatgcttgg tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg
    15841 gggagaagat ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa
    15901 gatcgatcaa aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc
    15961 ttgaaaccct gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa
    16021 cccctattcc aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag
    16081 atatcccgca aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca
    16141 aatatggcta tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca
    16201 ctcataatgt aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc
    16261 atccaagtca ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa
    16321 aagtagggat tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg
    16381 ctgaactgat cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata
    16441 ttacgatttt aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc
    16501 gagaaatttt agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat
    16561 taggttgtga gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag
    16621 aacgagtgaa atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc
    16681 tattagaaga gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta
    16741 agttgaatgt cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc
    16801 ctttacttgg tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag
    16861 aagtgccaga aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag
    16921 tttttgactc aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg
    16981 aaccagttta cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag
    17041 ataagtcttt aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat
    17101 atggcgaaaa aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg
    17161 tagctgcctc tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag
    17221 ggactccgtt tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg
    17281 aacgcaaagg tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag
    17341 taatcttaga atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa
    17401 atgaacaaaa tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt
    17461 cataaaacag tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt
    17521 aaaagacgat tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga
    17581 ttatcactgt cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac
    17641 agaagcttgg ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc
    17701 tgaagaaaaa atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac
    17761 cgtaccaaaa attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt
    17821 cttcggtatt gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa
    17881 tgaaattttg gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga
    17941 agtgatttgt acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca
    18001 tgaagaacca gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga
    18061 aaaagaagga tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac
    18121 cacctatcaa gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg
    18181 atgtcgtgtt tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt
    18241 attagaagag atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga
    18301 tgcttatctt acagaaacac tgatacattt gacccacttc tatcattctc ataattggac
    18361 gatgcagctg cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg
    18421 gcccgatacc ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt
    18481 attgaaccgt gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc
    18541 gaatgactat cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa
    18601 actgcagtta ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt
    18661 gacacaattt gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag
    18721 aagtttcctg tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg
    18781 cgaaatggtc atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca
    18841 agcgattagt tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag
    18901 taacagtaac gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta
    18961 gagaagaaga cggaccaaat
//
LOCUS       DAHUWM010000035        18558 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHUWM010000035 DAHUWM010000000
VERSION     DAHUWM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.035"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            177..2411
                     /gene="pcrA"
                     /locus_tag="NK788_000854"
     CDS             177..2411
                     /gene="pcrA"
                     /locus_tag="NK788_000854"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCH6769200.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2428..4464
                     /gene="ligA"
                     /locus_tag="NK788_000855"
     CDS             2428..4464
                     /gene="ligA"
                     /locus_tag="NK788_000855"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCH6769201.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4570..4875
                     /gene="gatC"
                     /locus_tag="NK788_000856"
     CDS             4570..4875
                     /gene="gatC"
                     /locus_tag="NK788_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCH6769202.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4875..6347
                     /gene="gatA"
                     /locus_tag="NK788_000857"
     CDS             4875..6347
                     /gene="gatA"
                     /locus_tag="NK788_000857"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCH6769203.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6344..7774
                     /gene="gatB"
                     /locus_tag="NK788_000858"
     CDS             6344..7774
                     /gene="gatB"
                     /locus_tag="NK788_000858"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCH6769204.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7784..8839
                     /locus_tag="NK788_000859"
     CDS             7784..8839
                     /locus_tag="NK788_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCH6769205.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8950..10323
                     /gene="rlmD"
                     /locus_tag="NK788_000860"
     CDS             8950..10323
                     /gene="rlmD"
                     /locus_tag="NK788_000860"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCH6769206.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            10326..10454
                     /locus_tag="NK788_000861"
     CDS             10326..10454
                     /locus_tag="NK788_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCH6769207.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            10894..11082
                     /locus_tag="NK788_000862"
     CDS             10894..11082
                     /locus_tag="NK788_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCH6769208.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            11160..12173
                     /locus_tag="NK788_000863"
     CDS             11160..12173
                     /locus_tag="NK788_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6769209.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(12240..13274)
                     /locus_tag="NK788_000864"
     CDS             complement(12240..13274)
                     /locus_tag="NK788_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6769210.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            13389..15614
                     /locus_tag="NK788_000865"
     CDS             13389..15614
                     /locus_tag="NK788_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCH6769211.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            15632..17515
                     /locus_tag="NK788_000866"
     CDS             15632..17515
                     /locus_tag="NK788_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCH6769212.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            17496..18470
                     /locus_tag="NK788_000867"
     CDS             17496..18470
                     /locus_tag="NK788_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCH6769213.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6245 a         3361 c         4032 g         4920 t
ORIGIN      
        1 tgatttttcc ttttttttcc attcaaacgc ctcgctttct tttaatttag cgcgaaatca
       61 acgaattgac aaggaaaaat ccataaaaag aaacaaatga ttttgaacta gctagtttaa
      121 tgaaagccaa gaaaatgcta taattaacaa gattgataaa agaaaggatg ttgttaatgt
      181 catccaaagc tgaattatta aacgggatga acccccgtca gaaagaagct gttcttcaca
      241 ctgacggacc attactgcta atggcaggtg caggaagtgg aaagacgaga gtgctgacac
      301 accgcattgc ctatttgatt gaggaaaaag aagtcaatcc ttggaacatc ctagcgatta
      361 cttttaccaa taaggctgca aaagaaatga aagaacgtgt caatgcgatt ctcgcatctg
      421 gaggcgaaga tgtctgggta tcgacttttc actcgatgtg tgtgcgtatt ttaagaagag
      481 atgttgattt tatcgggtat aaccggaact ttacaatcat cgattcttct gaacagctga
      541 ccttgatgaa acggatacta aaagaactga acattgatcc gaaaaaatat gatcctcgaa
      601 gtatattagg aacaatctct caagcaaaaa acagcctgca gacccctcag gattttgcaa
      661 aaatgcaagg cagttattac gaagagattg cggcaaaatg ttatgctgct taccaaaaag
      721 agcttcaata taatcaatgt atggactttg acgacctgat catgaatacg attcgattat
      781 ttgaagaaca tcctgattca ctcacgtatt atcaaaacaa attccattat attcatgtag
      841 atgagtacca agatacaaat cacgcacagt atacgctagt caatttattg gcaggtagat
      901 ttaggaacct gtgcgtcgtg ggagatgcag atcaaagtat ttacggctgg cgcggtgcag
      961 atatgcagaa catcttggat tttgaaaaag attatccaga tgctgcagta attttgctag
     1021 aacaaaacta tcgctccacg aaaaacatcc ttagcgcagc aaaccaagtc attgaaaaca
     1081 atagcaatcg aaaaccaaaa aacttgtgga cagaaaacaa agaaggaaac aagatcactt
     1141 actaccgtgc agataatgaa cgagacgaaa cccgttttat cgtagaccgg atgcaagaag
     1201 agattcgaag caatcatcgg aattacggcg actttgccat cttgtaccgg acgaacgcac
     1261 aatctcgtgt aatggaagaa actctgttga aagcaaatat tccttataaa atggttggag
     1321 ggcacaagtt ctacgatcgt aaagaaatca aagatatttt agcgtatttg aatgtgctgg
     1381 taaatcctca agattcaatc agttttgagc gtatcgttaa ttcgcccaaa cgaggcatcg
     1441 gaccaggatc tatcgaaaaa ctacgcagtt ttgcttctct tcatgaatgg ccactgcttg
     1501 aagcagcgca aaatgtggat ctggcaaata tcggtggaaa agcaggacag caattaggtg
     1561 catttggaga gatgatccaa gaagtcactc agatgatccc atacttgaca gtgacagaat
     1621 tgacgaaaga ggttttggat cgtagcggat atctagaaga cttaaaaatc caaaatacat
     1681 tagaagcaca agcacgcatc gaaaacttgg aggaattttt aacggtcaca caagaattcg
     1741 acaaacaatt cgaacagcaa aatgaagaag acgctgatgc accagaagaa aaactaacgg
     1801 tcttcttaaa tgatctggca cttgtttccg acattgacaa tttagaagaa gatgcttccc
     1861 aagtgacatt gatgactttg catgcagcca aaggattaga atttccagtt gtattcctga
     1921 ttggtctaga agagggggtt tttcctttat ctcgagcatt gatggaagaa agcgaactgg
     1981 aggaagaacg ccgtctcgca tatgtgggga tcacgcgagc agaagaagcg ttgtatttaa
     2041 caaatgcttt ctccagaaca ttatatggac ggacgcaata taaccgacct tctcgttttg
     2101 tagaagaaat cgatcaggag ttactggaaa tcgaaggcat gcgtcctacg cctaaaaaaa
     2161 cgcctgtatt cgctaaaaaa acagcatata gctataaaca gccagagaca gcggttgttc
     2221 cctctaaaag tgctactgga ggggaaaata acagctggaa acctggagat aaagtcaaac
     2281 acaaaaaatg gggtctagga acagtcgtcc gagtaagcgg gacgtcgaag gatctagaac
     2341 tagatgtggc tttcccaagt caaggagtaa aacgtctttt ggctgccttt gcgccaattg
     2401 aaaaagctta gtagtggagg gtaaatgatg gaacatgaaa tgacgattga cgaagctgct
     2461 aaacgagcag aagaacttcg tacaagactg aaccaatggt ctcgcgaata ctacgtagaa
     2521 gataaaccaa cagtggaaga ctatgtttat gataaagaat acgcagaatt agtagcaatc
     2581 gaggaacaat atcctgattt gatcacgtct gattctccca ctcaacgagt aggcggaaaa
     2641 gtattagaag gttttgaaaa agtcactcat gatattccgc tttacagttt gaacgacgtt
     2701 ttcagcaaag aagagctgat agcttttgat caacgtgtac aaaaagcagt gggacgtgta
     2761 gttgactatt gctgtgaact aaaaatagat ggactttccg tttctttgcg ttatgaagat
     2821 ggaaatttcg tgagaggtgc gactcgcggg gatggaactg taggagaaaa catcactgaa
     2881 aacctaaaaa ctgtccggtc ggtcccaatc aaattaaaag aaccaatgaa catcgaagta
     2941 agaggcgaat gtttcatgcc aaaacggtca tttgtccaat tgaaccaaga cagagaagca
     3001 gagggaaaag atatctttgc gaatcctcgg aacgccgcag ctggttcttt gcgtcagtta
     3061 gattctaaaa tcacagcgaa aagaaatctg gacacttttt tatacacagt agctgatttc
     3121 ggtcctatgc aagcaaaaac ccagtatgat gcgctagaag agctagaaaa aattggattc
     3181 cacacgaatc gggaaaaacg tctatgtcat tcgattgatg aagtgtggtc atacattgaa
     3241 gaatatcatg acaaacgtgt ggatttgccc tatgaaatcg atggaatcgt catcaaagtg
     3301 aatgaattct cgctacaaga tcaacttggt tttactatca aagcaccgag atgggcagca
     3361 gcttacaagt ttccgccaga agaagttgaa acgctaatcg aaaacatcga atggacagtc
     3421 ggtcgaacag gggtagtaac gccaacagct atcatgacac cagtccgagt agcaggaacg
     3481 acagtaagcc gtgccagcct gcataacggt gattatatta agttgaagga tatccgcttg
     3541 aaagatacag ttctgattta taaagcggga gacatcatcc cagaagtttc gcaagtcgtg
     3601 ttagataaac gacctaaaga cagcgaagaa taccaattgc ctacacattg tccagtttgt
     3661 ggaagtgaac ttgtccattt ggatgaagaa gtagccttac ggtgcatcaa tcctaaatgc
     3721 cctgctcaaa tgaaagaagg tttgaaccac tttgtttcaa gaaatgcgat gaatatcgat
     3781 gggttggggc cgcgcgtact agaacagatg tatgataaaa aactagtagc agatgttgct
     3841 gatctttaca aattgacaga agaagagtta ttgacgttgg acaaaataaa agaaaaatca
     3901 gcaaataata ttttgacagc aatcgacaac agcaaagata attcggtcga gcggttgatt
     3961 ttcgggttag gaatccgaca tgtcggagcg aaagcagcaa aaattttagc agaacatttc
     4021 ggtgatctag aaacattgag taaaagtgac tatgagtcga ttattgcgct tgatacgatc
     4081 ggtgatatca ttgccgacag tgtagtcact tatttcagta acgaagaagt ccatgaattg
     4141 atgaatgaat taaaacaagc aggcgtcaat ttcgaataca aagggcttcg aaatgcccaa
     4201 ttgcaagaag tcgagtctcc ttttaaagaa aaaacagtag tccttactgg taaactaaca
     4261 cgatttactc gtgaagaagc aaaagagacg atcgaaaatc ttggcgggaa agtgactggt
     4321 agcgtatcga aaaaaacgga tatcgtggta gcaggagaag atgctggcag caagctgacc
     4381 aaagcacaag aattaggaat cgaagtttgg acagaagatc aaatggcaga tgcattggct
     4441 aaaagtcgct cagtagaaga atgaaaaatg agttttttca gacaaatttc aataaacaat
     4501 ttcagacacg gcactcttgt gttaaaatag agagcaaagt ttttaactcg tttagaaaga
     4561 aggggtagta tggcgattag tgaagaacaa gtgaaacacg ttgccaaatt agcaaaactt
     4621 gcgttcgcag atgaagaact tgcaagtttt accgaccaat taggtaaaat catcgatatg
     4681 gttgaaatgc tggaagaagt agatacggaa ggtgttccat ttacgtcgaa tgtggttgaa
     4741 acagtaaacg tgatgcgaca agatcgggcg caagctggat ggaaccgcga tgaattattg
     4801 aaaaatgtgc cggaaacaga agacggcttt atcaaagtac ccgcaatcat cgataatggg
     4861 gaggctggcg cataatgaca gaattacaca atcgttctct agaagaactg catcatatgc
     4921 ttgtatcgaa agaaatcact gtacaagatt tgacaaaaac aacatttgaa cgaatcaaag
     4981 aaacagagcc tgaggtcgag gcattcatta cattgaacga agaaaaagca ttggaacagg
     5041 caaaagcttt agatgaaaaa gggattgatg aaagcaacgt ccttgctggt attccaattg
     5101 gtatcaagga taatatcgta acaaaagata tcttgacaac cgctgcatct aagatccttt
     5161 acaattttga accaatttat gatgcaacgg tcatggataa aatctatcaa gcagatatga
     5221 tccctgttgg taagctaaac atggatgaat ttgctatggg tggaagtaca gaaacttctt
     5281 atttcaagaa aacaaaaaat gcttgggacc caacaaaagt acctggtggc tcttctggtg
     5341 gttctgcagc agcagttgca gctggacaaa ttccagtttc tttaggtagt gatactgggg
     5401 gaagtatccg ccaaccagca gcttttaacg gcatcgtagg gatgaaacct acttatggcc
     5461 gtgtctcacg ttttggtttg attgcctttg cttcatctct tgaccaaatc ggaccaatga
     5521 cccgtacggt gaaagataat gcattagcat tgacagctat cagcggttat gacgaaaaag
     5581 acggtacttc ttcaggcgtt tcagttcctg attttgcaga gggactgaca ggcgacatca
     5641 aaggaatgaa gattgcttta cctaaagaat acttaggcga aggtgtggcc cctggcgtaa
     5701 aagaagctgt attgaaagca gcagaaacat tccgttcatt aggtgctgtt gtggaagaag
     5761 tcagcttgcc tcattcaaaa tacggtgtgg ctgtttatta tatcattgcc tcatcagaag
     5821 caagttccaa tttgcaacga ttcgatggta tccgctacgg ttatcgctct gaaaatgtga
     5881 aatcattaga tgatgtatat gtcaactcac gttcagaagg attcggtgaa gaagtaaaac
     5941 gccgtatcat gttaggtaca ttctccttaa gcgcgggtta ttatgacgct tacttcaaaa
     6001 aagcaggaca agtccgtaca ttgatcaaac gagattttga aaaagtcttt gcagattatg
     6061 acttgatcat cgggccatct tcaccaacag ttgcattcgg aatcggtgag aacattaacg
     6121 atccaatcac aatgtacatg agtgacatct taacgatccc tgtcaacttg gctggacttc
     6181 caggtatgtc tgtaccggca ggtttatcag aaggattacc agttggattg cagatcatcg
     6241 gtaattattt tgatgaaaaa acgatgtatc aagcggcata tgcgtttgaa caagcaacag
     6301 aattccataa acaacaacca gctattttag gagggaaaga cgaatgaatt ttgaaacagt
     6361 cattggacta gaagtccacg tcgagttaaa aaccaactca aaaattttct ctccagcgcc
     6421 agctcatttt ggtgcagaac caaacagcaa tacaaacgtg atcgactggg gttatccagg
     6481 cgtgctgcca gtaatgaata aagctgcgct ggaattcggg atgaaagcag ctttggcatt
     6541 aaactgtgaa atctctaaag atactcattt tgaccggaaa aattactttt acccagataa
     6601 tccaaaagca tatcaaattt cccaatttga tcaaccaatc ggtcatgacg gatggatcga
     6661 aatcgaagta gaaggtaaaa agaaaaaaat ccggatcgaa cgtgtccatt tagaagaaga
     6721 tgctggaaaa aacatgcatg gtattggcgg ttattcttac gttgatttga accgacaagg
     6781 cacgcctttg atcgaaatcg tttctgaagc tgatatgcgt tcaccagaag aagcatacgc
     6841 ttatctagaa gcattgcgat caattatcca atttacagaa gtcagcgatg tgaaaatgga
     6901 agaaggctct atgcgctgtg atgccaatat ttctcttcgc ccttatggac aagaagagtt
     6961 cggtacgaaa gcagaattga aaaacttgaa ctctttatct ttcgtgaaaa aaggtttagc
     7021 ttttgaagaa aaacgtcaag caaaagttct tctttcagga ggagaaatcc aacaagaaac
     7081 acgccgtttt gacgaaacaa cgaataaaac agttttgatg cgtgtcaaag aagggtctag
     7141 tgattatcgt tacttcccag agcctgatat cccgaaattc gtgatcgatg acgaatggat
     7201 tcaaaaagta aaagctagtt tgcctgagat gccagcttct cgtcgcgaac gctatatccg
     7261 tgagtttgga cttccagagt atgacgcaat ggtattgaca cttacaaaag aaatgtctga
     7321 tttttttgaa gctgttttgg aaaatggtgc agatgccaag caagcatcta actggttgat
     7381 gggtgaagtt tctgcttact tgaacagtga gaaactggaa ttagctgaga caaaattgac
     7441 acctgagaac ttagcaggaa tgattcgttt gatcgccgat ggaacgatca gctcaaaaat
     7501 cgccaaaaaa gtcttccgtg aattgatcac aaatggcgga gaagcccaag aagtagttga
     7561 aaaaaatggt tgggtgcaat tatctgatcc gaacaaacta ctgccaatca ttaatgaaat
     7621 tttggataac aaccaacaat ctgtcgatga tttcaaaaat ggaaaagatc gtgcggttgg
     7681 cttcctagtc ggtcaaatca tgaaagcaac taaaggacaa gcaaatcctg gtgtcgtgaa
     7741 taagctactt aaagatgaat tagcgaaacg atagaggctg aaaatgaaaa aagcacgcgt
     7801 aatttataac cccacctcgg gaaaagaatt attgaaaaaa aatttggcgg atatcctaca
     7861 ggcattagaa gaagcaggat ttgaagccag cgcctatgca actacaccag aaccagattc
     7921 agccaagaat gaagcatgta gagtcgcaga actaggtttt gatctagtag tggcagctgg
     7981 tggcgatggc acaatcaacg aagtggttaa tggaatcgct ccgcttgaac accgtcctaa
     8041 aatggccatc atcccagctg gtacaacgaa cgactatgct cgagcattaa aaattcctag
     8101 agataatatt aaagcagcag cagaagtgat aaaaaaacac caaaccgtaa aaatggacat
     8161 cggcctttct gataacagtt acttcatcaa tatcgctgca ggtggctatt tgacagaatt
     8221 gacttatgaa gttccatcag aactaaagag tatttttggt tatcttgctt atcttgcgaa
     8281 aggagcagag atgctccctc gagtaaagcc aatcaaaatg cgcttgaagt atgatgaagg
     8341 ggaatatatc ggaaatgctt cgatgttttt cctagggctg acgaactctg ttggaggatt
     8401 cgaacagatc gcgccagatg ccaaattgga tgatgggaag ttttctttaa tcattgtgaa
     8461 gacagcaaat atctttgaga tcttgcatat tgcagcactc atgctgaatg gcggtcgaca
     8521 tgtcgatgat ccaagaataa tctataccaa aacaagcagt ttgttcgttg aaacagaaga
     8581 tgatatcaat cgaccagtaa tgatcaactt agatggtgaa tacggcgggg aagcaccgat
     8641 gcatttccaa aatcttcatc aacacattga gttctttgcc aataccgatc agattcctga
     8701 tgaagcaatc acaggtacag atgaagaaga attagaggtt gtcagcaaag aattcgtaaa
     8761 agaagtcgaa cgtttaacgg atgaagatat cgacggtgac ggaaaaatag cagacgaaac
     8821 acctaatcaa aagaattaat caaaagccgg tgctgaacgt agtaaggtca gtgccggttt
     8881 tagttgtgta attagctgat ttatagtaga gttcagtagg attgaaaaat aggcttaaag
     8941 gagcaaaata tgacagaaca gcaaatagta aagaaaaacg aacgatatac agtcgaaatc
     9001 gtcgacttga gttatgaagg aatgggggta gcaaaaatcg atggttatcc attatttatc
     9061 gaaaatgcac tccctggaga agaagtagaa atactagtag taaaagcagg caataagttt
     9121 ggatatggaa aagtcgaaac atttattaca caatcacctg atcgtcagcc tgtagaaaac
     9181 aatgcattac ttcgcatagg tatcgcacct ttagcccatt tgaagtatga acaacagctt
     9241 gtcttcaaac aaaaacaagt agagcatgta atggcgaaga ttgctaagat gccagaagtt
     9301 cctgtacttc caacggtcgg aatggcagaa ccattcggct accgtaataa agcacagatc
     9361 cctgttcgcc gtatcgatgg acagttagcc accggttttt acaaaaagaa tagccatgat
     9421 ctagtcccta tcgaagattt ttatatccaa gatcctgtca tcgatcaagc aatcaaagta
     9481 gtcagagata tcttgcaacg gttccaagta cgaggatata atgaagaaaa aaatgaagga
     9541 cagcttcgtc acatcattat ccgtcgtggt cattattccc atgaaatgat ggtcgtttta
     9601 gttactcgta aagaaaaatt ctttaaatcc aaggaaattg ctgaagctat tcatgaagaa
     9661 ttgccagaag tggtttcggt catccaaaat atcaatgaag aaaaaacaaa tgtgatttta
     9721 ggagacaaag aaaaagtttt atatggcaga gactatatcg aagatcagct attaggaaaa
     9781 acctatcgaa tctcagcaaa gtcattttat caagtaaata ctcaacaagc agagtatctg
     9841 taccaaacag ccatcgagtt tgccgagtta gataaaaaag acattgtgat cgacgcttat
     9901 tcaggaatag gtacgattgg cttgtcttta gcagacagag tggataaggt gtatgggatg
     9961 gaagttattc cagaagcaat tgaagatgca cagttcaatg ctttaaataa taaaatcgaa
    10021 aatgttcatt atgaagtcgg aaaagcagaa aaagttatga aaaaatggca agaaaaaggg
    10081 atcaaaccat ctgtgatcgt agttgaccct ccacgaaaag gattagatca tcattttatt
    10141 gaatcagcaa tcgaaatgtc accagaacga atcgtctata tctcatgtaa tccggcaact
    10201 tttgcaagag atgctaagtt atttgcagaa gcaggttatg ccgtgaagaa agttcagcct
    10261 gtagatcttt tcccgcaaac ccatcatatc gaattagtcg cttcattcgt taaaggagaa
    10321 taaaaatgga ccaaaaagaa aaaatgaaag aattgatcaa aaagaaacaa ggtggaggcc
    10381 gatccaaaca aatggaaaca cctaagaatg atcgtaaaaa catgagaaaa ggccctaaaa
    10441 tctttaataa atagacgtgc taaagaaaga ctaagaagtg ttaacttttg ttattgtttc
    10501 ttagtctttt tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg
    10561 tgcaaaatca aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca
    10621 atttgattaa ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac
    10681 taaatataca tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga
    10741 cgacgtagca gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg
    10801 atagtgaacg tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg
    10861 tcttagattt tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac
    10921 cggatctacg aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt
    10981 ggatttgctt caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt
    11041 ttgtcaaaag cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg
    11101 ataggaaata acactatggt ataatcagaa tgcttaatac taactactga gggagcctta
    11161 tgaaaaagaa aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt
    11221 ttgcaatctt tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata
    11281 tgttttcaaa tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg
    11341 tggcgaaaaa aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct
    11401 actatccaaa aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat
    11461 tcgtcggcgg agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca
    11521 acatcgcagt ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag
    11581 ttcttcaagt gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc
    11641 tagacatgga acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat
    11701 atgcagctat acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc
    11761 caaaagatag tctgaaagga accatttctt attgcggacc tgttgatttg aagctaacag
    11821 cacagcagca ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt
    11881 tgattggaac aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc
    11941 atgtttctaa ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg
    12001 agcaaggtca agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt
    12061 tttatacaga tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag
    12121 aatcgcaaaa ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg
    12181 tgaaaaagcc gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt
    12241 caataccttt ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc
    12301 aaaatgattg gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct
    12361 tgcccaattg atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt
    12421 gtctcgatat tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt
    12481 ttcaatgccc gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga
    12541 agaggggatt cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag
    12601 ccacctagat gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag
    12661 gcacgcatcc tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact
    12721 ttatatcccc cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg
    12781 gttttagtca tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg
    12841 ttatccacga cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta
    12901 acagtcccaa caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta
    12961 tcagatagat tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc
    13021 cctactcgca aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag
    13081 atcacaccaa tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc
    13141 aacgcagcag ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga
    13201 tcgttattca acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct
    13261 ctaattccaa ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt
    13321 gtttagtaaa cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga
    13381 gtgaaatgat ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa
    13441 agagccgagt cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa
    13501 gcaatgtatt ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa
    13561 attatccccg aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc
    13621 tgtcgaaaga tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc
    13681 ccgcatcgat cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc
    13741 attatattac agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca
    13801 agtcagaggc acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg
    13861 atttgttcta taccatattt actgatagag cagtgatcac acgatctgta aagataagaa
    13921 atgaaaccgg agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc
    13981 atactaggcg atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga
    14041 tttcacggca atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca
    14101 gtcaccatat gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag
    14161 aagcgatcgg acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc
    14221 agatcaatca attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga
    14281 atgcaggcca gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga
    14341 acaaaatgag tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc
    14401 aatttgctga acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata
    14461 gtgaaaaaat caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat
    14521 tagatgatgg ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg
    14581 agtatgaagg caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag
    14641 gattgaaatt tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc
    14701 gaacacatcc tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac
    14761 aacatgtact ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa
    14821 agattttaga tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag
    14881 atgtctattc gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc
    14941 tcgggcttta tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag
    15001 gctgttctgg tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat
    15061 ggacaagtga taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg
    15121 cttatccgat ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca
    15181 gatctacttc gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac
    15241 tggatttgac aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct
    15301 atcaaacgat tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg
    15361 aagaaaatat tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt
    15421 tagggaggat actagcttca gctcaaccag cgttccatga agtatatctg atggggttag
    15481 atgatgaggc actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga
    15541 tgacagttgg actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt
    15601 tcaaaaagtt atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga
    15661 tccacaagtt tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac
    15721 aagggaagaa gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt
    15781 ttgttattcg ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc
    15841 ctgttctttt gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat
    15901 ccattacatc aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac
    15961 gacaagccct gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg
    16021 ttcgtatttg acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca
    16081 ttttgctggt gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc
    16141 cgaagattcg ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat
    16201 tctagctgtg gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt
    16261 ccgatttttt gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga
    16321 agatctttgg atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga
    16381 tttgtctttt gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga
    16441 tgggaaggaa atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga
    16501 tggatttgaa aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt
    16561 ggagacgata gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg
    16621 taaaatcgaa atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg
    16681 ggtaaatcga catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga
    16741 aaaagatttg gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc
    16801 caaccagatc acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac
    16861 gaacctcgaa tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt
    16921 accaggtgca gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga
    16981 aacattaaaa aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga
    17041 agaaaacatt cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca
    17101 ttatgaaggg aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg
    17161 catgtatgcg ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt
    17221 catcttatgt gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat
    17281 gacgttgctt gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca
    17341 ggcgatccaa gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt
    17401 tgacgataaa ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca
    17461 agaaaaaccc gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc
    17521 atatggatac ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg
    17581 ggaatacgca ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg
    17641 gaaaagaatg gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg
    17701 acagaggtag cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa
    17761 aagtcaaaga aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag
    17821 taaataatca atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg
    17881 aaatcaacac agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta
    17941 aaatgcttgt caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg
    18001 gtttaaggtt cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg
    18061 aaacatatcc ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc
    18121 ccgtttcccc ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag
    18181 aactaacaag aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc
    18241 gatttgaaca gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac
    18301 tggaacaggc aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt
    18361 ttgcaaaagt aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt
    18421 accgaatcag cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag
    18481 attattattt gaaaaacgga gtttacaaga caaagctgct ttattctata tgtcatgaac
    18541 aagagagtaa aaaagttc
//
LOCUS       DAHUWM010000036        18426 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHUWM010000036 DAHUWM010000000
VERSION     DAHUWM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.036"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(224..1516)
                     /locus_tag="NK788_000868"
     CDS             complement(224..1516)
                     /locus_tag="NK788_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6769214.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1513..2136)
                     /locus_tag="NK788_000869"
     CDS             complement(1513..2136)
                     /locus_tag="NK788_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769215.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2139..2975)
                     /locus_tag="NK788_000870"
     CDS             complement(2139..2975)
                     /locus_tag="NK788_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769216.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2992..3879)
                     /locus_tag="NK788_000871"
     CDS             complement(2992..3879)
                     /locus_tag="NK788_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769217.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3887..5122)
                     /locus_tag="NK788_000872"
                     /pseudo
     CDS             complement(3887..5122)
                     /locus_tag="NK788_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5805..6488)
                     /locus_tag="NK788_000873"
     CDS             complement(5805..6488)
                     /locus_tag="NK788_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCH6769218.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6506..7123)
                     /locus_tag="NK788_000874"
     CDS             complement(6506..7123)
                     /locus_tag="NK788_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCH6769219.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7133..7957)
                     /locus_tag="NK788_000875"
     CDS             complement(7133..7957)
                     /locus_tag="NK788_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCH6769220.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7950..8510)
                     /locus_tag="NK788_000876"
     CDS             complement(7950..8510)
                     /locus_tag="NK788_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769221.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8684..9640
                     /locus_tag="NK788_000877"
     CDS             8684..9640
                     /locus_tag="NK788_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6769222.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9945..10901)
                     /locus_tag="NK788_000878"
     CDS             complement(9945..10901)
                     /locus_tag="NK788_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCH6769223.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11005..11607
                     /locus_tag="NK788_000879"
     CDS             11005..11607
                     /locus_tag="NK788_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6769224.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11695..13194)
                     /locus_tag="NK788_000880"
     CDS             complement(11695..13194)
                     /locus_tag="NK788_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCH6769225.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13210..13734)
                     /locus_tag="NK788_000881"
     CDS             complement(13210..13734)
                     /locus_tag="NK788_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCH6769226.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14032..14781
                     /locus_tag="NK788_000882"
     CDS             14032..14781
                     /locus_tag="NK788_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCH6769227.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14772..15557
                     /locus_tag="NK788_000883"
     CDS             14772..15557
                     /locus_tag="NK788_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCH6769228.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15526..16242
                     /locus_tag="NK788_000884"
     CDS             15526..16242
                     /locus_tag="NK788_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCH6769229.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16344..17378)
                     /locus_tag="NK788_000885"
     CDS             complement(16344..17378)
                     /locus_tag="NK788_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCH6769230.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(17382..18425)
                     /locus_tag="NK788_000886"
     CDS             complement(17382..18425)
                     /locus_tag="NK788_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCH6769231.1"
                     /translation="MFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILSK
                     VVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQT
                     TLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAK
                     NIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPM
                     DYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTAT
                     NILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWI
                     VVLQMIHGIGLFS"
BASE COUNT         5515 a         3882 c         3245 g         5784 t
ORIGIN      
        1 ttttgtaaaa tctaatgatt tttaatgcat tattcctttt tgttcgggtt taaatagtta
       61 tttttatttt ctcctatcta aaaaagcagc ccacataaaa agaaggcatg acaaaaagca
      121 tgtcatagcc cctttttccg aataaatagt gttcaaaaga cagcctatcg gcaataaggc
      181 aagattttat aaactacggg aagtaatttc cgtaaatcta ttcttattct tttaagctga
      241 caagctcgtc aatcactact gcttttcctg aagcaataga atgatttgct gcgattcctg
      301 tcaaaatcga caaaatacca tctacgtgag ttgctgcccg attccacgga tcttcttctt
      361 tttcttcacc aaatagatca tctaataaac gcggatctcc tccgccatgt ccgcccgctt
      421 cttgttcgac gattacctca tatggctcac cgaacatcgg atagacatgg atggactgaa
      481 gaacggctgc tccttcatcc tttttatctc cacctgcatt gatgtaagac ttttcgatca
      541 ctttcatttc gatccgtcct ttgctgccat tgaaagaaac attgaatcct tcccacggca
      601 tatacgcgtt tagcgaatac gtcaaaatcg caccagtttc atatttgacc gttacgccta
      661 gtgtatcttc aatagagata tcatcggaaa atacactgcg gtcacgaata tatccactgt
      721 ctttttctgc ttctaaatac atttctcgca attcctcatt ggctgacagg tctatagcaa
      781 atggatcagc tttagctagt tcgcttccat agcatcgact gtaaaatatt tctactccgc
      841 gtttctccgc attatgcatc ccataaaagt ttaatccacc gaatgcaaat accttttggg
      901 gacggctgtt caaccaaaaa ttcactagat caaaatgatg ggtagattta tgaacaagaa
      961 gcccgccgct attgtgctta tttcgatgcc atcttctgaa ataatctgcc ccgtgttgcg
     1021 tatttaacag ccattcgaag tggatagaaa acacatcacc aatcacatca ctttctatca
     1081 gttcctttat cttcgtgtga tacggtgcat aacgataatt aaaagtgact cgcaggcttc
     1141 ttcccgtctg tttttggacc gttattattt cttgagcttt ttcctcatcg atagtcagag
     1201 gtttttcgca aataacatca tatccttttt tcattgcacg aatgatataa tcatgatgcg
     1261 ttcgatctac tgacgtaata atgacttcag ccggccgtac gtcatccagt agttgctcaa
     1321 attgtgatgg catacatgtc tttacttttt tctgtccata cttgttcaca agtatccgat
     1381 ttgcataatc catccttgtt tgagatacat cacaaaaacc gactagttca caagtttgat
     1441 gatacgtaga ggcaattgcc tcataaaaaa aacgcgctct cccaccaagt ccgacttgta
     1501 catatttttt cttcatttaa agtcattctc cttttctttt aagcaaaata ctgtgacaaa
     1561 tggcagccct ggatacagta atgcagtgag taaaaaatca ggctgtatac accagaacag
     1621 tccatacaaa aggcataaat accacagaaa ccccttgacg ttttttgaca acagccagat
     1681 attccttttg atcaaataaa caagactatc tgctttatct tctttgcaga tatcaattag
     1741 cgtactaaaa aaactatggg atacaaaaag caacagacac aatagaaaag gaatcagata
     1801 ccaagctcca atctttccca ctaataaata ccaatcaact gcaaaaataa tgacaaacag
     1861 ctgaaacaac gtaatgatga aacacatccg tttgattttc cctttcctcc aacatacctt
     1921 tttttctgcc aagtaatgga cagatagata ataagaagga tacatcatca aaaaataaat
     1981 cagatagaca acacgagcag acagcactaa tcctaaagtg aacaaacatc ctagaactaa
     2041 agcgaaaaga gcattactga taagactggt taaaaaaatc cgacagacat agtagttgag
     2101 cttgtaaaga aacatctctt ctattggctt tttcatattc agcccttcag accgcttgta
     2161 ctgatcccct ggacaagata tttattgaag aataagaaaa tcagaaaaac cggtagcagc
     2221 gacaacgtag acatcgcaaa catactcgcg taagtcgtac tagatccagc gtctgcaaat
     2281 aatttgatgg caatcgatac cgtgtaagat ctcggcttgt ttaaataaat caatggtccc
     2341 atgaagtcgt cccatttcca ataaaactgg atgatgatcg tcgtgatgat cgaaggtttc
     2401 agcaaaggaa aaataatttt ggtaaagaca ctatacttgc tgcatccgtc aataatcgct
     2461 gattcatcta actctttggg gatgctgacc atgaattgca tgatctggta aatgaaaaat
     2521 gcttgtccaa aaaaatgtgg taaaatcagc ggcacatacg tcccaaccaa attcagccga
     2581 ttgtaaataa taaactgcgg aatcaaaatc acttgtgcag gaatcatcat cgttactaac
     2641 atgacgacga accagaatct ttttcctttg aaatcgattc tcgataatgc gtaagcaata
     2701 caagtcgagc tcagaactgt ccctatcgtc gaaactgcag agatgacaaa cgaattccta
     2761 aagaaagtaa aaaatgaaat tccagcaaac cccgcccatc cttttgcgta attttcccat
     2821 tgaaaagtct ctggaatcaa attcagcgaa ccactcataa tctcttgatc cgttttaaaa
     2881 gaacctgcga tcatccataa gaccggataa atcatgacta gacctaatag caaaataaaa
     2941 ccgtattgaa accatttcgt tactgtatta ttcatttctt cctcctctca ttcacacaac
     3001 gacccatttt tttgatgatt tgaaaatcag aaaggtgaca gctcccataa tcaacagcat
     3061 gatccacgaa atagcactag cataccccat agaattaaaa gaaaaagctt ggttatacac
     3121 ataaagagca taaaagttcg tggcatcatt cggaccacct tgggtaatga taaatgcctg
     3181 cgtaaaagtc ataaacgccg cgattgtctg cataaccaag ttatacaaaa tgatcggcga
     3241 aagaagaggt aacgtgatct tgtaaaacgt ctgccatttg ttcgctccat cgatctctgc
     3301 cgcttcataa tacgagcctg ggatctcttt gatacctgca gcaaagatga tcattgccga
     3361 accgaattgc caagcagaca tcaaaacaag agggatcatg accagttttt gatcgctaaa
     3421 ccaggaaagc gcaggtgcgc ctaatcgttc caggaaaata ttcactaacc cgtttttaga
     3481 aaacaactcc cgccaaacta gagcgataga aatactgcca ccgatcaaac taggcacata
     3541 atacaaagaa cgaaagaaag tcgttccttt gatattcctc gttaaaagat aagcaatcaa
     3601 tagaccaaag atcagtttca caggtacaga tgcaaataca tagagaaaag tgactcggat
     3661 ggagttcaaa aaacgggcat cactcagcat ctttatataa ttatccaagc cgatccatga
     3721 gactggattt cccaaacgat aatcggtgaa cgaataataa agtgacaaga tcatcggtat
     3781 cactgttatc aatagaaatc ctaggatgaa tggagaagca aatacgtaac cgaccacatt
     3841 gtcttgatcc aagaaccgtt ttagtttttc ttttttcatg ccttccttct gctgttactt
     3901 cttcataaat gactttttca actaaaagat tgtattggtc ttgaatttcc ggattcaaag
     3961 cactatcaaa gacattcact tcgccgttgt ccatatcgga aatcatttct aaataatcaa
     4021 atgtcgcttg caaagttgca tcatctgcac gagcgagctg ttctcgaata ttcgacatga
     4081 ttggtacacc acgttctcct tttaaaattt cattggcttc ttcactgttt tgaaagaaat
     4141 caataaactt agcagcttct tctttatggg ctgagctttt tgccatactc agcatttgcg
     4201 aagaacgtaa agggatacca gattctccat ctgcttttct gcgaggtgga ttaatcaact
     4261 gaatctccat atcttcagga gcccctgaca atatttctgt caactggtta cttgatagaa
     4321 acaccattgc cgcttctcta tccctaaccg cctgaaaaga cagatccgtc gtacttgcag
     4381 ctccaccacc tggttctgga tgggcccctt tgtctaccag atttttacgc agctggaaaa
     4441 aatctgttag atagtctgga ttttcaaatc ccaagctgtt agatagatct tcccgattat
     4501 aaaaattcaa tcctttctca acttgtggta tttgaataat acagccagct ataaaatctt
     4561 cgaatcttga aatagcatag attcctaatt cttcagtgat ttgatagctg atttgttcaa
     4621 attcatccca tgtccaattt gaagctggtt cagggatatt taattgttca aatatatttt
     4681 tattgtaagc aatcgcaaac gaattcaccc cgttagacag tccaactaat tgcccgtcgt
     4741 aagtcgtcgt tgctaaatag gattcatctg cattagaaat atcaattatc ccttgctcca
     4801 catattcatc taatggttca atctgattga tgtattgagg aaattgattg ttcagttgga
     4861 aaatgtccca gatatcatct gatgctgcta gtgtacctaa ttttgtgtaa tatccttcat
     4921 aatcataaaa ttcataatcg atcgtcacat ggggattttt ttcttcataa agctcgatta
     4981 cttttactgt tgcatcgtgt cggacttgcg aaccccacca agccatgttc agcgtgattt
     5041 ttttgtcatc ttcttgccca caagccgtca agaagaataa cataacactt aaagctaaga
     5101 tgattctcat tcttttcttc attctctgca cctctacttc cattgttatt tttcaatata
     5161 ctgtattttt atacagaaca tatttattaa acgatctttt tcaaggggaa taaaaaccaa
     5221 gacttgcctt atcgttctgc ctaggatgaa caactcaata ctttcaagaa aaattagata
     5281 gtgctgattc ttctttcatc gctaaaaaca cttcttgctt caatgatggg aggataatta
     5341 cgcaaagaaa aaaggatatc agacaactgc ccaatatcct tgacatagct aaataagcat
     5401 gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata actagtaatt ctgtatgaaa
     5461 ttaggtacaa tatacacgtc gatgcacaag cattgactat ggcaacttga ttggtcagtt
     5521 cccgctgact tttcaagccc cgacaattta gctgctacta aattgtcggg tgcctttttt
     5581 agtttttatt cgtttttcat tctatagaaa gcgcttcaat aaatcaagac atttttttgt
     5641 tagaatgcta ataataacaa aaggttttaa ctcttcttat gtacattaca ggtaaaaaaa
     5701 caaaaaaatt tctctttttt ttgaattctg aacaaatagt actggagtac aaaagacgag
     5761 gaagagccac tatctgctct tcctcgtctt ttgattctca cctgttattc atgattcttt
     5821 ttctcagttt cccgatattt ttccatttta cgactaaaaa gctctccgct acttggtggc
     5881 gtatatgtga tggcatttgc tcctgcttga atggtctcag caatactttc ttccgttgga
     5941 ccacctgtcg caatgatagg aagttcagga tattttttac gaaaatggcg tactgcttct
     6001 gcagtttctt tacctgcact gatattgata atcgaaatac cagctgctaa cttttcatct
     6061 atttctgtat atttcgacgt taccgtactg ataactggaa tatcaactac tcgacaaact
     6121 tcttttactg tctctaaagg cgtaggaccg ttcaaaacga cccctaacga accttgcgct
     6181 tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg tcaaaccacc acctacaccg
     6241 gagaaaactg gaatgtcagc tgcctcaata atgcttttag taatcgctgg atgaggtgta
     6301 aaaggataca cagcaattac tgcattcgca tttgtattac gtatgatcgc aatatccgta
     6361 gtaaagatga tcgaacggat caattttcca aatatcttga tcccgcttgc ttctaaaatg
     6421 atttccggta ctcttacgat atcttttctc aattccgtca tgatttctgg cgtaaattgt
     6481 tctgccatca gccacttctc ctttgctatc tccggtcata ctgattcaat tgatacgtct
     6541 cgatgacctg gatgattttt tgcgcataac cggggtctgt cgcataacct gcatcttgca
     6601 atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac tttttcatac agcgcaggat
     6661 tccaatctac tccttggacg aacagttgtg tatggtcatc catggactgt tcccatgaat
     6721 catacacttt gaagtctcct tggatagtta tccattcttc gttaacaaat tcctttgttt
     6781 ccaggcttac tttcttctga tccccataag ccttgatccc gaaaagatta ttgtatttgc
     6841 tagccaaggt actttttccc caatttgatt ctaatatagc ttgtcctaaa atgatactag
     6901 gaagcactcc ataaccatct tgtagttctt gggcatgcgg aacaagccgt tcaataaatt
     6961 gctgatggga ttgcgcttca ttttcttgct gattatcagt aaaaggagaa gacagattct
     7021 tcaaggaaaa aacaaaggct aagagtatca ctgctacacc ggcaaccact gctggccaac
     7081 ggatattctt gcgcttttgc tttttccact tttttttcgc catgatcaat tcctactttt
     7141 catttaattc attcagatat tcttctagcg tcaattggtg tttggtgata tactcagcgt
     7201 tttctttacc gacataacgt aagtgccaag gttcatatgt gatcttagtt atatcttcgt
     7261 gtccttttgg ataacgaacg ataaatccat actgcgcagc attatctgcg atccatttag
     7321 ctccaggctg atctccgtaa cttgcatcca atacagtaga aggataactg ttgtaccagt
     7381 tttgatcgac gacatcgact gccagacctg tgtggtgttc gctgtatcct ggctccgtaa
     7441 tcgtttcttt agtaatcttt gtcgcctcct cttctgatac accttgacga ctcatcacat
     7501 cttggacatt ttgttcgaaa acttgttgtt gggatgatac agaccggtaa gctgagacca
     7561 tcactaatgg aaatccagct gcttcagcag ccttggcaaa tgcttcatat tctggagcaa
     7621 tgcgtttgtc gatcaagtat ccgttactta atgatgccaa ttgagtagac tcatcaattt
     7681 ctttttctaa tttgtgatct ggaccaacca aaactagctt ccaatcgtct gttttaacat
     7741 ctggcaattg tttggacgta cgatcttttg tttctttgtc ctttaccttg ctgctggttg
     7801 tttgactgtt cttttcttta ctagcagttg ttttggtgct tgttcgttcg actgcttgat
     7861 tgccggcttg aacatgcgaa tcatcatcgc taaaaataac atagcccatt gctgcgatca
     7921 taatcacaac tgctccaaat cctaaaaatt tattcacttg aaaaatcctt tcgttgttct
     7981 tcacgttccc cattcgcctc atgaacgtaa gtttcttcca gattttcatt cacccaatcg
     8041 agaagttcga ttttttctcc ttcgatctgt ttttgaacat ctgctggcag acgaaaattc
     8101 aaaatatcat catagcccca ttcatcatat tgaatactta ctttcaagtc aaggtatcta
     8161 ccagcctgct gattattttt caaagggatc agctcgactt ttgctgcttt gctgctaagc
     8221 catttttgcg cgatgcgtgt tttgatttgc ttgtactctg caacggcaac gtttcgtttt
     8281 tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta gcatccattc ataatcagtg
     8341 aaaagatttt ttactttctg catcgcttca gatggcagat ttttctcgcc atctttccat
     8401 ttttgccatt gttctggact gatcagcaag tattcatcta aaaaattttt ttcagtttca
     8461 aactgttcta ataatacact cataataaga tcaatatata cttgatgcac gtttatcccc
     8521 tccttttaac tattctactt aagttttgat caaaaagcac ctcttaaatc gctgctttca
     8581 ttttttttaa tgtttcaagt cctctcctct ttattcttcg tgatttttgt tataataata
     8641 gacagacaag aaactcaatc caactatgaa ggagcgtttt cagatgaaaa aatatcaatg
     8701 gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc gaaagcttca atcaagagac
     8761 gagcgaacta acagcagttg cttccagaac ttcagaaaaa gcggaaaact tcgctcaccg
     8821 ttacaatatt ccaaaagctt acggaagcta tcaagaaatg ttagacgatg ctgaaatcga
     8881 catcgtgtac attgctgtac caaatagaca gcatatcgac catatcttgg cagcattgga
     8941 agcaggcaaa cacgttctat gtgaaaaagc catcacgatg aataaaaaag aactggctga
     9001 cgctatgcgc ttagcagaag aaaagaattt gatcttagca gaagcgatga caatcttcaa
     9061 catgccactt tatcagcagt tacgctcaat aatggataca ggaaaactgg gggctttaaa
     9121 aatgatccaa gcacctttcg gcagttacaa agaaccagac cctaaaaacc ggttcttcaa
     9181 tccagaatta gctggaggag ctctgttgga tatcggtacg tatgctgtat ctttcgctcg
     9241 tttctttttg agctctcaac ctgaagtagt tgcctctaca atggttcctt ttgaaacagg
     9301 cgtggacgaa caatccgtca ctatcttgcg taacaaagaa aatgaacttg ctgctgtcag
     9361 cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt gcctttgaga acggttatgt
     9421 gacgattgct gattacccgc gtgctgatcg tgcggagatc ttatttacag atggtacaaa
     9481 ggaattcatc gaaagcggga atacatctga agcaatgaat tacgaaataa agaatatggt
     9541 acacatgatt gaaggtgact tgcccaatcg ctctctcttc ttgacaaaag atgtcatcga
     9601 aatcttggat caaatgcaag ttctatggaa aaatatgtaa ttttaaaagg ttgtgaaaaa
     9661 gctgttctgc atcaagtaat aagaacagcc aaacaaaaat agctcctcct atttttcgtt
     9721 tggctggact tattaccgca gaggaaagct tttgaacacc gtttaagaaa gaggttgtga
     9781 gcctaccgtt cagctccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt
     9841 tgaatattgt gacttgtgac cgaggagttg acaggagaac accgtttaaa gaagaggttg
     9901 tgaaatcagc tttttgacct gaatattagc tgaattcaca acctctattt ttggattttt
     9961 tgttgggaga attctgcctt gatttcaata aaacttttaa aagactgcaa aaactggaac
    10021 aattctgctc gatcctcgaa ttcagggcga ctagtcggca gaggcatttg ccgatacaat
    10081 acataaactt cttcaatacg tgttaaaatt tgtctgccat catttgactc tgaaaatgtt
    10141 tctgcagtat aaatcaataa accatagata tgttttccat aataagctgg ttgctggatt
    10201 ctctctagat tttccagcat gtctttaatc acattggcct gtgctcttct catagagaag
    10261 taagtttcgt agtatagcgg ctgtctgagc cagtagtttt cttgatgttc tctggcatct
    10321 tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt tgatctgcac atcttcttta
    10381 ttttctgata aaagaaattc tgccatttgt ctcaaaatat ttcgaaattc tttttcgatc
    10441 tgtatttggt tttccttcag ctgcttcgta cgatctggca tgtaaacatt tgcaagcaaa
    10501 gcaaatacta atccaatcgc cattaatccc gcttcattgc cgatcagctg ccaagacatg
    10561 ttttcctcca ctaaataatg agtgaccaaa accgaattta ctactatccc ttcggttaat
    10621 ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa gactgaagcc aaaaatagta
    10681 tacccaacag caaaaaaact gaacagtgct gttgccgtag ctaatataaa ggctgcgatt
    10741 cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc ctacacttaa taatgcaatg
    10801 atccctgcag ctggccaata caacagcgat aagctacccg ctgtgaccat cgaaaagaag
    10861 acactgatca ctgttttgac tgttcgcatt cctatacgca tttacttccc ccttttttat
    10921 tttaattata cttgaataat gaaaaactag ctatcagaca aaagtttagg tatgatagta
    10981 aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt ttcgactagg tatgcgtacg
    11041 atcaaaagtg ccttagctgt ttttttatgt atcttgtttt ttcatttgac tcaacgagga
    11101 atcccgatga ttgctgcatt atctgctgtt ttttctttgc gtcaggactt gactacaacc
    11161 gtttcttttg gaaagtctag gatcatcggc aatacattag gtggtacatt agctatcatt
    11221 tatttttttg tcaaaagcta ttttcacaat gattttttgg tagaattgat cttgcttcct
    11281 attctagtta ttattgtgat cgtcgtgtca gatggtatta acaataattc cggtatcatt
    11341 tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc ctcaaggcga atccgctttg
    11401 tatgctatcc aacgtgtact agatactttt atagggacat tcattgcaat cggtatcaat
    11461 ttctttcttc gtcctcctga aacagaaaag aaggaagaac tatcagaaga tttggtcgaa
    11521 ctccaaaaga aagaacgatt gctaagaaca aatttacaag agatagaagc taagatccaa
    11581 aaacaaaaga aaaatagcga ggactaattt tcttttgcaa taaaataaaa aaatggagag
    11641 aagaacttta aaaagtcttc ctccattttt gattgaacgg tgaagtgtac tactttattc
    11701 ttccttcatc gagttttgtt ctgcctcttt tggcgcttca tacatattta cttggctagt
    11761 agtcccacgt gtcgacaaat tctctacgat ttccttcaag tcaagtcctg ttgtcgcttt
    11821 tagtgtttct tgagtagaag aaagcaaatt tgtggcataa tttgtcactc ggttcgctcc
    11881 actggtttct cctgaactgc ttcctgtatc gacaacagag attttttcga tatttcctag
    11941 cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg ataaccatac tcaagacagc
    12001 tgcttcgcca tattccttga aggcattggc gatcttttgt tttgcttcgg cttctgcttt
    12061 tccttttgct agtgctgctt cggcttctgc ttgtccagct aaacgtgtct tattggcttc
    12121 cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg gcttcagtca cttctttgac
    12181 tttttgagct tgtgcttctt gttctttagc gtaccgatcg gcatctgctt ttttcttcac
    12241 ttccgaatca tattgctttt cacgacgtgt gatttctttt tcttccagtt cgatttgttt
    12301 ttggcgttct acgattttaa tctgcatttc ctgttcgatt acttgttgtt gtgctcgggc
    12361 actttctaaa ttgtaggctt ggtctgcttt tgctttagca acatcttgtt cttgtttgta
    12421 ggttgctaat ttcagttctt tttcttttaa tgattctgcg atctctgttt gtcgctgtaa
    12481 ttcagctgct tgtgattctt tttctgcttg agcttttttg atacgcgttt cttttagagc
    12541 ttccgcttct gctatatctg catctctttt gacttgggcg attcttggtt tccctaaaga
    12601 gtcaagataa ccgtttttgt ctctgacctc tttgatagtg aaagaaacga tgatcaaccc
    12661 catttttgca aggtctacag aagcgacttc ttgtacgctt tgactgaact tatcgcgatt
    12721 ttgatagatt tcttctacag tcatagaacc aagaatcgag cgcaagtgtc cttctaatac
    12781 ttctcttgct tcattttcca gttcttctct tgtcttgcct aaaaactgtt ctgctgctgt
    12841 agcaatctct tctacagatg aaccgatttt gatgatcgaa gtaccatcac acatcaccgg
    12901 tacgccttgt tcggtataca cttcaggggt ggagacgtcc aatttactcg ataatagact
    12961 gatccgattc gaacgttgaa aaactggcaa gacgaatgcg ccacctccac gcacaatttt
    13021 gattttgtta ttgctttcat caatgtgcac atttttgctt cctaaataac ttccactgat
    13081 gatcaacgct tcatccggtt ttgctgtctg gtacttcgct acaaagacga tcagcaacat
    13141 cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca actggtattt ccatttaaca
    13201 cacgctcctt taagctttta tcatgctttc ataaggcatg acataacaga ttctttcctt
    13261 gatctcaatg atcaatactt tcgttccccg ctcagctgtc tgttcttggg ggctgtaaaa
    13321 acaagctgga cgactaatgg aaccggtaac acttttcaac tgaatttctc ccatacctcg
    13381 tacagggatc ggtgtgatca caacagctac ttgtccttcc agagtttttt cagaagacga
    13441 catcgttgat tcggcatgtt tcatcggcat caatatatag aaattaagca aaaataccaa
    13501 cactatcgat atgcctccac tagcaataaa taaaaataaa gatgattgat ctgttaaacg
    13561 ctcacctaga aaaccaaata agcttgtgaa agttaaccaa gggataataa gcatcggatc
    13621 aagcgggcca tcaaaatcga atacatctcc aaaaataatc aataggactg ccaatcctgc
    13681 acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg attcccccta tcatatacac
    13741 gcctccttcc atttctacgt ctatcatacc aatgtttgaa aattctgtat agcttctttt
    13801 accgactcag tggtgttttt tataggaagg ttgtgtttat cgttgacaat ttgttaaatt
    13861 tttgttttca tgttaaaatt ttcacaataa attctttcga ggtagcctat gtttcctttt
    13921 tttaagaaaa aaccattaaa gctccagaac acgatcatcc ttgtcgtttg tttctccaca
    13981 ttcgtttctt tgattgtttc tgccatattg atcaggaatt ttattctttc gatgaaccga
    14041 tctagtgtgt cagataaaat ctctagcatt gctaaaatcg ttgccaatga taaactcatc
    14101 caaaaaaagc tgcagactgg tgacccgaca catgcgattc aaggaataac aaatgaaatc
    14161 atgattgcat cgaatttaga ttttatcgtt gtcatggacc ctgaaggcat tcgatattct
    14221 cacgcagacc caagttacat cggtcttcct ttctcaagtc atgaagatgt gaaaaaagtg
    14281 attggaggac aagaattttt ctctgaaaaa gaaggaatgt tagggttcgg cttccgctat
    14341 ttcgtcccta tttattatga tcaagagatt attggtgcag tttgtgttgg cttaacaaac
    14401 aacacgattg acaatcaagt caaagatgga caaaaaatga ttgttttttc attgattatt
    14461 ggacttagcg taggcatcat aggcgccgtt tttcttgccc aaaaaatgaa aaaagtactg
    14521 cttggacttg aaccacaaga aatagcagaa cagttaagtg aaaaaacgat tatcgaaaat
    14581 gaagtagatg aaggaatctt agccatctct tctgataaaa ctatcctctt gatcaataca
    14641 cctgcaaaaa acattttcta ccaaacagag cagctattgg atttcgaagt aggaaaaaaa
    14701 tcagtgataa tctatatgac gttttgttta aagagacgat tgagcatcaa acgaccgttc
    14761 ggactcaatc catgtattta aatgggatag aaactatttc tagcgttaca cctcttttta
    14821 gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa tcgatcagaa gtaatgaaat
    14881 taatggagga gcttgggggg acgaaacaat atataaactc catgagagct caaacccatg
    14941 aattcatgaa taaacttcat gtcatttctg gtttgattga tttgaagaag tatgaagaag
    15001 tcacccattt tatccagcag ctaaatcaaa attataaaaa agagattggc caagtctcaa
    15061 gtctgatcaa agtacctgca attgccggtt ttttattagg caaaataaac gaagccaaag
    15121 aacaatctgt tgaaatgctg ttagaaagcg attcttttat ccctaaaatg gagatggatg
    15181 actccatcca ttcacttttg caagtgttag ggaacttact tgataatgca aaagaagccg
    15241 tgctacaaaa acatgaaaga aacggacagg tccggattca tttaaactat gacacagaag
    15301 ctgaagtatt catcgtgacc gtctgcgatg gcggtgtcgg gatatcagaa gaaatctcag
    15361 aaaaaatatt tacgctaggt ttttcgacga agggaatcaa tcgtgggttt ggattgaatc
    15421 tcatccattc aatcgtcaaa cgccacggtg gatttttaga attttccaaa caaacaaatg
    15481 gcggtacaat cgtctatcta gaattaccaa aaaaggaaga tcaaaatgat gacaaatgtc
    15541 ctgataatcg aagatgatcc aatggttgca gaattgaatc gtacttttgt ggaaagcatc
    15601 cctaatatga acgtaatcga aagtttcaga aatgtagaag aaggaaaaaa atatttcaga
    15661 caaaaaaacg cagatctgct gctgcttgat gtatacatgc ctggcgaaag tggtatccat
    15721 tttttatctt ggcttagaca gcaaggaaat gatatcccag tgattttgat cactgctgca
    15781 gatgatatcg aaaccataaa gaatgtgatc cgctacggtg ccttagatta tttgatcaaa
    15841 ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt atcttgcttt ctctcagaca
    15901 atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc aagtattcaa ccacaccact
    15961 cccattcacg aaataccatc attcatcgat ttgcctaaag gtctgtcaaa actgacttta
    16021 aaaaaaataa gtgcatttat tctaaaagaa gatactattt tttcaacaaa agaactggct
    16081 caaaaaagct cactgtcacg tatatccgtc aagaaatatt tgaatttttt agtagaacaa
    16141 gatatattga acgaaagaat caaatatttg gaaatcggcc agccgctaac ctgttatgaa
    16201 ttgacaaaga atgggcggaa aaagctcaca gattactgtt gagtcatcga attatcccat
    16261 acaaaatagt ccaagtatac agaagtaaca actgtttact tggactattt ttaaacattt
    16321 tattgacgaa tatccatgtt ttcttatctt aattgacggg ctttaggaag agatacctca
    16381 tttttcgttt ttccagtttc tattatttca cgaaagttct cgaaacttgt cgaaatcata
    16441 tttgataatg cctcatctgt atttgaacct acatgcgggg ttacaagaac tctagggtac
    16501 atttctacta attgctggat agcagggtct ggaatcgtct cccatggctt gaaggatcgg
    16561 aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa aaccagctaa atgattgctt
    16621 ttcaaagctc ttaaaatagc ttcattatct tgaagctctc cacgggctgt attaatcaag
    16681 atgctacctt ttttcatttg agctaaaaat ccttcattga tcatttgatc gttttcacct
    16741 gggatatgag gtacatgaat actgacaata tcgcttttgg ctaataactc ttcaagcgag
    16801 tggaacgtca atactttttt agcagcttct gtctgatgaa tatcgtagcc gatcactttg
    16861 gctcctagtc ccttaaacaa aacagcttcc gtcaacccta tcctgcctgt tcctatgatc
    16921 cctactgtgc aatttctgat ttcacgacta aacatttgct catccactcg aaaatctttg
    16981 aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa gcatcatggc aaaagtcaaa
    17041 gataactctg ctatcgcatt tggcgaatac gatggtacac gggctactgt cataccaaaa
    17101 tcagctgccg cttgtagatc gatatgattg actcccacag tccgtgtaaa aacataacga
    17161 atcccatatt cctgcatttt tgcaagattg gtctcatctg ctatacaatt tcccctcaac
    17221 aatacagcat catggtcctt tgctgacacg atattgtcat gtgtcaacag ttcttcataa
    17281 agactgagat cataatggta aatattcaat ttttcaaaaa aatctatctc gttcggacga
    17341 acaccgtaac atgcgattct catactttct tcctccattt cttatgaaaa taaacctatg
    17401 ccatgaatca tttgtaatac aacgatccat acaggtgtca gtaccacagc aaaaatagtt
    17461 gacagtaagg atgcattgga ggctaaaagt gcctccctat caaaactgat agcataggct
    17521 gctgccactg ttgcagctgg tgtagccatc atgataacga ttgttgccag tgcttcaaaa
    17581 ttgactggta gaatattcgt tgccgtcaaa actgctagaa tcacgatatt caatgcaggt
    17641 acaaatacca ctttgactag actgtaatac catgaagttt tttcagatgc agcttttttg
    17701 aagcttactt cacctaatgt tgcgccgatt gctagccacg ccaatggtga acttaaacca
    17761 gctaaataat ccatcggctg aaatagccac ggtgcagtct ggtcaattcg caaaaacgct
    17821 acttggctta tctctccttc tgcacctgta actgacactt gtggcagata cccttggaac
    17881 agccaaatga aaaggccggc aaaagtagcg ataatgattg gattgacgaa catcgctttg
    17941 atatttttag cttccatctt caagccactc atttttatgt atccataaga atacaaaaag
    18001 attcggtagc caatattgaa aattgaggaa tacatcacgc cgacaggacc ataaatcgca
    18061 ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga tcgtcaggac acgcaacgtt
    18121 gtttgcttat cccctttata gcttaagaaa aaaggttttg aaactagaat aagaataata
    18181 taaataacta atccccaaat caagacattg attccttgca tcagtaattc tgtattgata
    18241 tcttgcatga aagctcgaaa tgccagtgct ggcaaagcca ctgttaagac gactttcgat
    18301 agaatttttc caacagattc agaaaaaatc cccttttttc tacaaacaaa ccctaataat
    18361 ataataaaaa ctgttgaagt gattgcactg acaatcgaca tatctgtcaa agtcgatcga
    18421 aaaatg
//
LOCUS       DAHUWM010000037        18152 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHUWM010000037 DAHUWM010000000
VERSION     DAHUWM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.037"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(290..1087)
                     /locus_tag="NK788_000887"
     CDS             complement(290..1087)
                     /locus_tag="NK788_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCH6769232.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="NK788_000888"
     CDS             complement(1084..2268)
                     /locus_tag="NK788_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCH6769233.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="NK788_000889"
     CDS             complement(2293..3540)
                     /locus_tag="NK788_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCH6769234.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="NK788_000890"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="NK788_000890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCH6769235.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="NK788_000891"
     CDS             5520..5834
                     /locus_tag="NK788_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCH6769236.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="NK788_000892"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="NK788_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCH6769237.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="NK788_000893"
     CDS             6519..7163
                     /locus_tag="NK788_000893"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCH6769238.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="NK788_000894"
     CDS             7178..7507
                     /locus_tag="NK788_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCH6769239.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="NK788_000895"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="NK788_000895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCH6769240.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="NK788_000896"
     CDS             8495..9319
                     /locus_tag="NK788_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCH6769241.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="NK788_000897"
     CDS             9312..9659
                     /locus_tag="NK788_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCH6769242.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="NK788_000898"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="NK788_000898"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCH6769243.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="NK788_000899"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="NK788_000899"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCH6769244.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="NK788_000900"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="NK788_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCH6769245.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="NK788_000901"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="NK788_000901"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCH6769246.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15289..16083
                     /locus_tag="NK788_000902"
     CDS             15289..16083
                     /locus_tag="NK788_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769247.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="NK788_000903"
     CDS             16073..18115
                     /locus_tag="NK788_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769248.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5658 a         3336 c         3778 g         5380 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaactttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaattttt tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggcatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagaa ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg atttttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa at
//
LOCUS       DAHUWM010000038        17701 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHUWM010000038 DAHUWM010000000
VERSION     DAHUWM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.038"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="NK788_000904"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="NK788_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCH6769249.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="NK788_000905"
     CDS             complement(2019..2921)
                     /locus_tag="NK788_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6769250.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="NK788_000906"
     CDS             complement(2918..3610)
                     /locus_tag="NK788_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCH6769251.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="NK788_000907"
     CDS             complement(3653..4294)
                     /locus_tag="NK788_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6769252.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4601)
                     /locus_tag="NK788_000908"
     CDS             complement(4332..4601)
                     /locus_tag="NK788_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769253.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4574..5263)
                     /locus_tag="NK788_000909"
     CDS             complement(4574..5263)
                     /locus_tag="NK788_000909"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCH6769254.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="NK788_000910"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="NK788_000910"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCH6769255.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="NK788_000911"
     CDS             complement(5983..6915)
                     /locus_tag="NK788_000911"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCH6769256.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="NK788_000912"
     CDS             complement(6912..7688)
                     /locus_tag="NK788_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCH6769257.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="NK788_000913"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="NK788_000913"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCH6769258.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="NK788_000914"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="NK788_000914"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCH6769259.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="NK788_000915"
     CDS             complement(12013..13293)
                     /locus_tag="NK788_000915"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCH6769260.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="NK788_000916"
     CDS             complement(13312..14238)
                     /locus_tag="NK788_000916"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCH6769261.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="NK788_000917"
     CDS             complement(14251..15534)
                     /locus_tag="NK788_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCH6769262.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="NK788_000918"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="NK788_000918"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCH6769263.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="NK788_000919"
     CDS             complement(16322..17233)
                     /locus_tag="NK788_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCH6769264.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="NK788_000920"
     CDS             complement(17230..17694)
                     /locus_tag="NK788_000920"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCH6769265.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
BASE COUNT         4918 a         4014 c         3154 g         5615 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttcaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 a
//
LOCUS       DAHUWM010000039        17697 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHUWM010000039 DAHUWM010000000
VERSION     DAHUWM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.039"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..152
                     /locus_tag="NK788_000921"
     CDS             <1..152
                     /locus_tag="NK788_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769266.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="NK788_000922"
     CDS             complement(230..763)
                     /locus_tag="NK788_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCH6769267.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="NK788_000923"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="NK788_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCH6769268.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="NK788_000924"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="NK788_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCH6769269.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="NK788_000925"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="NK788_000925"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCH6769270.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="NK788_000926"
     CDS             complement(4488..5066)
                     /locus_tag="NK788_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCH6769271.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="NK788_000927"
     CDS             5388..5858
                     /locus_tag="NK788_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCH6769272.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="NK788_000928"
     CDS             complement(5966..7192)
                     /locus_tag="NK788_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCH6769273.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="NK788_000929"
     CDS             complement(7946..8146)
                     /locus_tag="NK788_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6769274.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="NK788_000930"
     CDS             complement(8280..8888)
                     /locus_tag="NK788_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCH6769275.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="NK788_000931"
     CDS             complement(8973..9443)
                     /locus_tag="NK788_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCH6769276.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="NK788_000932"
     CDS             complement(9491..10405)
                     /locus_tag="NK788_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCH6769277.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="NK788_000933"
     CDS             10551..11354
                     /locus_tag="NK788_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCH6769278.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="NK788_000934"
     CDS             11532..12479
                     /locus_tag="NK788_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCH6769279.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="NK788_000935"
     CDS             complement(12547..12840)
                     /locus_tag="NK788_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCH6769280.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="NK788_000936"
     CDS             complement(12868..13533)
                     /locus_tag="NK788_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCH6769281.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="NK788_000937"
     CDS             complement(13673..14305)
                     /locus_tag="NK788_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6769282.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="NK788_000938"
     CDS             complement(14312..15379)
                     /locus_tag="NK788_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6769283.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="NK788_000939"
     CDS             complement(15376..16107)
                     /locus_tag="NK788_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCH6769284.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="NK788_000940"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="NK788_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCH6769285.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..>17697)
                     /gene="mltG"
                     /locus_tag="NK788_000941"
     CDS             complement(16891..>17697)
                     /gene="mltG"
                     /locus_tag="NK788_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCH6769286.1"
                     /translation="YYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPEGYDIDQI
                     AERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEGYLFPATY
                     DYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEGVKENDRK
                     NIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTGYGPGPFD
                     NPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5252 a         3588 c         2841 g         6016 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg ttgatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataata
//
LOCUS       DAHUWM010000040        17231 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHUWM010000040 DAHUWM010000000
VERSION     DAHUWM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.040"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            34..471
                     /locus_tag="NK788_000942"
     CDS             34..471
                     /locus_tag="NK788_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769287.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            566..1450
                     /locus_tag="NK788_000943"
     CDS             566..1450
                     /locus_tag="NK788_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCH6769288.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1674..4841
                     /locus_tag="NK788_000944"
     CDS             1674..4841
                     /locus_tag="NK788_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769289.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4861..5406
                     /locus_tag="NK788_000945"
     CDS             4861..5406
                     /locus_tag="NK788_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCH6769290.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5434..5889
                     /locus_tag="NK788_000946"
     CDS             5434..5889
                     /locus_tag="NK788_000946"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCH6769291.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5886..6644
                     /locus_tag="NK788_000947"
     CDS             5886..6644
                     /locus_tag="NK788_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCH6769292.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6730..7470)
                     /locus_tag="NK788_000948"
     CDS             complement(6730..7470)
                     /locus_tag="NK788_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6769293.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7531..8784
                     /locus_tag="NK788_000949"
     CDS             7531..8784
                     /locus_tag="NK788_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCH6769294.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9106..9333
                     /locus_tag="NK788_000950"
     CDS             9106..9333
                     /locus_tag="NK788_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCH6769295.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9456..9782
                     /locus_tag="NK788_000951"
     CDS             9456..9782
                     /locus_tag="NK788_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCH6769296.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9850..10563
                     /locus_tag="NK788_000952"
     CDS             9850..10563
                     /locus_tag="NK788_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCH6769297.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10873..11724
                     /locus_tag="NK788_000953"
     CDS             10873..11724
                     /locus_tag="NK788_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6769298.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11845..13710
                     /locus_tag="NK788_000954"
     CDS             11845..13710
                     /locus_tag="NK788_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6769299.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13758..15167
                     /locus_tag="NK788_000955"
     CDS             13758..15167
                     /locus_tag="NK788_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCH6769300.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15275..15976
                     /locus_tag="NK788_000956"
     CDS             15275..15976
                     /locus_tag="NK788_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6769301.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16135..16614
                     /locus_tag="NK788_000957"
                     /pseudo
     CDS             16135..16614
                     /locus_tag="NK788_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5752 a         2896 c         3710 g         4873 t
ORIGIN      
        1 acaatatcaa aaaatgcacg tagaagtaga taaatggagt ggttttgcaa atttctttta
       61 tttctagaag ttattcacgt atacggattt gtttttctca caaataaaaa ggcaggaagt
      121 acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt gttaggcaca
      181 ctaattatag cggggattgt cgcatttatt attcaaggcc gtgacatatt aacagtacat
      241 caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc tgataacaaa
      301 aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct gccacctaag
      361 atttttactt tattaggtat acaatgccta tcattttctt ttatttccta tatcattgtt
      421 ttaattttcc tcagtttcat agtatggatg ttttatcttg tcaaaaaata aatgaatgtt
      481 tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt atattaaaga
      541 aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa accccagttc
      601 aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg aatcggtgtt
      661 tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact ttactcgcga
      721 agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa cagtcaacga
      781 aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt tcccattggg
      841 tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg aagcgatcaa
      901 tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg acgactattt
      961 tatgaatgtc gtcgcaattg gctctatccc tgaatcaatc aatgatgtag atgcggagaa
     1021 gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt tagcttccac
     1081 acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg agagcagtac
     1141 cttgctgata ggactaacta actcagtcgg cggttttgaa acattacttc caaatgcaaa
     1201 agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga tcgatacgct
     1261 taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc tagcctacct
     1321 tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga cgaatgttga
     1381 tggagatgaa ggggataaat tacctgtgac aatcaaagta ttgccctcac atttacaggt
     1441 ttattgttaa aatggaggtt gtgaccgagg agttggcggg agaacaccgc ttgttcggga
     1501 taagggactg tgacaagact tttgtcacag cctttttttt gtacaaaaaa gaaatttaca
     1561 cctaatagaa aaatttttcc ataaaaatac atattttaaa tatgaaagta caattggaaa
     1621 tagattaaaa cgataaaata aaattgtcaa aacgtcagag aggatggtgg agaatgaatc
     1681 aatgtgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg atggtgacat
     1741 ccgtttttgc cgatgtagag aaaacaaacg aatcaacgca atcagagaat caaagttttg
     1801 acaagactac tttttcagaa gaatcgatta cttctgaaac tacagaatct caaagtgagg
     1861 cttctactaa taaaaggcaa gaagaaaata agacaagtga gacctcagaa gtagctgaac
     1921 aaacgtctga tagcagtgaa gcggcactgg caagagctgt ttcagcggat gggtacagcg
     1981 aagctgccac aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta actaagatcc
     2041 gactgattca gcctttgact ttagacaggg aattagagat caaacgagat atcgttattg
     2101 attttggtgg attcgctcac aatttcggca cacaccatat ttatatcaat gagatacctc
     2161 agtcgatcga gttccagaat tttaaaggaa cagctgcaca tccaggtgga ttgacaccaa
     2221 atatcgatgg aaatgccatt atcattgctt atatgaccga tttttgggga agcagatatc
     2281 attttacggg agaaataaaa tttaccggga cacttgattt gtatgatggg tccaaattag
     2341 gactgattta cgctcctcgt gcaactgtca cattagatgg tgtttccggt gtgttggatg
     2401 tccaaccagt gaaggaagga ctgaatgccg ctccgggtaa agcctatttt tgtagaagct
     2461 atcaattgaa tgtgatcaat ggtagtcaac tttatggacc gtatctaggg aaattctacg
     2521 gttttctaga ttcaggagat gagactggta gtggaaaaac agcgccagga atccatatct
     2581 taagcggttc aaaggtctct cttgattatg atcgggcaga tggcgttagc gatgaaggag
     2641 aggcagtcga tacgctacct ggtaatgtca tgtttgaagt cagtggttcg ggaagcgaat
     2701 tttcggtaaa tacaaagatc aatattacca atgacaacaa tcgcggaatc atccaaatga
     2761 gaggttcagg atcgaaagtc actgtatcga atggtgggaa aatcaccatc aatacagcaa
     2821 ctacgggcgg atttcgcttg caaggagatt ctagtcaaat ccatgtatca agtggtggac
     2881 aaatctcggt caaaatggct ggagatgacg atcaacccgg gaacaatggg atgcgttttg
     2941 taggaaaagg cctttctttg atcgtagaag gagccggatc agttatcgac atcgataagc
     3001 agagtgggcg ttccagcgca gttcgttttg agaatggtac gcaaaagctg acagtaacca
     3061 atggaggctc attgagggta agaaatacag gtgatggcaa atcttacgtg aacagactca
     3121 atcaaggaaa tcaagcgatc caattttatg acgcttctgg ttttctagaa agtccaggtt
     3181 cagctgattt tacagtcact ggagaaaaat caaatatcga tatacgcgca gattccggag
     3241 caactgtaga tacaactgga gatattgatt taaattttac ggcaggtgaa aaaacttatt
     3301 tagtcatggt cgggaaaaca ggggaagata agaccggcat catcagtggg gatgtgttga
     3361 atgtggactt agaatctcct acctattttg atttccaaaa caaacggatt ggaagcgaga
     3421 ccaatggcat aggcggctgg gtattccaag gaaatggaga ttcttcttta aagatcgccc
     3481 attcagaaat cgctttatgg cgaaaaacag gcgcaacatt cgataacttt gaaaaagacc
     3541 cggattattt ttctgatatg acagacttat cgatcaatgg gaccgactta ggcaatttcg
     3601 tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa
     3661 attataatcg aatcagtgcc aataatcagc tcccgatgat tgaatcctta agagtgccaa
     3721 cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc
     3781 caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttac gcagatacga
     3841 cgaaatcatc tgtagaaatt actgctctca cgcaaggaca aacagatgaa aatgaagggg
     3901 caggatataa cccatgggga gaaggcgaac aaggcggctt gttcgaagtt caagtaccca
     3961 atgatgagag attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa aaagtaagag
     4021 gtggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg attcctcctg
     4081 aacctgctaa tttaagtacc acattgatca atgcagcttc tcgcacgatc acaggtacag
     4141 gtgaagcagg agccttggtt tcattgaaaa aaaatcaagc tgttttgtca gaaacaacta
     4201 ttgatgatac aggggaattt gttctttcta tcccagaaaa tcaacttttt gcaggagatg
     4261 tattagaaat tgtattaaac gaccaagcag gagaagctac tgctaagggt gtgcagaaca
     4321 agccaagtac caacgatgaa attgggaatc acaatccaag tgatacgcca gttgtttatc
     4381 atgatgcccc gccttttgca cctgctacga agatcgtagt agaaggtggt ctctcatttc
     4441 ttgccccttc caaactcgat tttggtcaga tcaaagcaac tggtttgaca caagaagcat
     4501 ttggccaaat ggatatacag gaaaaattat ggatttatga tcagcgagaa acgaaaaatc
     4561 cgtggatctt aacgataaaa ttatctcagc ctttcacaaa tgatgcagga ttttctatgc
     4621 aggatgtcct atatttcaag aaaggagagg agtatcaatt agtgaatgaa gaagaactgg
     4681 aaattgtcag aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa tatttaaata
     4741 acggtcgggc gtttaaactt gtcttaacca aagaaaatca gatagctggt aattactcag
     4801 cagagataac atggacatta gcagatgcgc cgacagaata aagatcaagg aggatgattg
     4861 atgaatcgaa tatggaataa aagtcatcga aacaactgct ctttacttat gatccttctc
     4921 cttggattca tcctttggca gacacctgtc catgcctccg aattcaattt tgcggtagat
     4981 tttgaattac cggctaatca gattgataag aacaaaacat atataaatgt aaggatggag
     5041 cctgggaaaa cacaggaaat cccctacacg gtctttaacc gaacagataa atcagtaaca
     5101 gttgagacat tgatccactc cgctacgacg aattcaaacg gtgtgatcga atatggagaa
     5161 tcaaaaagta aatttgatga aacattaagt tatgatatga aaaacttagc aaaaacagtg
     5221 ggagaaataa ctgttcctcc tcaaagtgcg gtcactgaga cactttcact gaaagcgcca
     5281 gaaaaagagc tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa aaaagagaac
     5341 gtttctaaca aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa agcaatcttg
     5401 atttgaaata gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc ttatgcggat
     5461 caaagcgatg gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc ttcttatatg
     5521 ttcggcttga ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc ttacctttca
     5581 ataaaaaaag aaaaaataga tgtagcaccc aatacaaatt ttgatttact tgtcccatta
     5641 gaaggaaaaa aattagctcc gggacattat caagcggttg tgaagggtgc ttggaaagaa
     5701 aaacgctgga atctccatac cgattttgaa ataactgatg atgcagcgga ccaattgaat
     5761 gaaaaggata ttgatttaaa aaatcagcca gaaacatttg actggcgatg gctgatactt
     5821 gttggagtca ttcttattgg cttatttgct ggattattta ttttaaaaga aagaaggaaa
     5881 aagaaatgaa aaaaacaatt gcattaagta ccgttctagc tggaagtcta ttattatgta
     5941 cacaaagtgt ttttgctgag aataagagct cagccactac agaaggaacg atttctgtga
     6001 ccaaaccaga tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt gatccaaatg
     6061 atcctgatac tccttttaca cctgacgaac caaatcaggg aacatccgga ttactgacga
     6121 ttgaccaagc tccagatctt gattttggaa caatcaagtt aggtacagca aaaacagtat
     6181 atgctgcatt acaaagcgga acggacagta ctggagcaca aacacaagta cagaatttcg
     6241 tacaagtaac ggataaaagc ggaaattatg ccggttggaa attatcagtc aaacgaacag
     6301 aatttattgg tacaacatct gatacgaata aactaactgg ttcaaaggtg aaattcaaaa
     6361 acgccgtgat tcgaaatagc gcaactaata gcagtggaat gccatctacc gtttctggaa
     6421 ctggcgctga aggaatcgaa atcccagcag atcaggcagt agatctagta actgcaggag
     6481 caaatgaagg gattggcaca tggatttatt cattaggaga gaatgtggaa caagggagac
     6541 aatcagtgga attatttgtt cctgtatcca attatgctgt agacaactac gtttcaacat
     6601 tcacatggtc gttaacagat gctcctacag aagaggaagc gtaaaatcat atcaacgatt
     6661 tcagggagtg gagccgattc aaggcccact ccctgaaatc tgctatgctt acgtctcagc
     6721 tcttattcat caactcgaag cagttttttg gaagtcatgg aaacgttcct ttgtcttttc
     6781 aaagatcttt cgaccaacga gtaaggagtc accgttgaaa aattctaaca cataatgagc
     6841 agaatcaatt ttctgcaatt gcgataagtt gatacagtaa gattttaaaa gtaaaaagta
     6901 agcatacttt tctaactctg tcttcaattg ttttaaagaa gtattcacta atttatcttc
     6961 tgtagttgtt ttgaccataa cttggttttt caaacctttg atactttcaa tatataaaat
     7021 ttcccgagga tacaggacga gacttccacc tttttgatca ataaagattt tatcgacatt
     7081 tacgatctgc tgttctttcc aattgatctg ttctagcact tgacgaattt gttcagataa
     7141 attcctgtct tttctgatat atccaatcgg aaggattttc cgattgatga tctctactgc
     7201 aaaattcgta taagcggtga aaaaaacaat attactatgt gcattttttt tacgtatttg
     7261 ttcggcgaca tcaatacctg tgagtgattg cttcaagtcg atatcgataa agaaaacatc
     7321 gtgattatca atagtgccca actggatcaa ctgattgatt tgagagatag aagtgagctg
     7381 cacgatattc tcgatatgat gattgaccaa ttctctgatg atccattcgc tgtgaaactg
     7441 gttatcttct atgataaaat actttttcat atgcaggctc ctattaaaaa aatatgtgta
     7501 cattttgaaa caaaacaatg gaggaataag atggtcgcaa cagtgacact ttatttgacc
     7561 tttactttga ttttagattt aggtatctat ttattctttt cgaaacaact gctagtaaaa
     7621 aacagctttg gttgtatagc tttgatggtg gtgacacttt tacttcctat ccctaaagtg
     7681 agctttcctt acctagttgg cttattattg atgtttcttt tttattttac tacgaaaaac
     7741 ctgtttatca gctgtttgat cagtaatctt ttttttgcta tcactggact cagctttttt
     7801 ctggtttttg atctgatgga ttatttagga aagagacatc ttggaacagc aagttttttt
     7861 gcactactta tcgcatttat tttctatcta ttagtaaaaa gtatagacaa acagacgaga
     7921 tttctcaact tgctttttac ttatccaact gaacactatt ttggaggtat ttgtatttta
     7981 ttaagctggg gcgtgattta cttggtattg ggaagtgttc acttccgctc atctaattat
     8041 ctgcttctat ctcttttgtc gatagtcatc agtttttttt ctttgttttt ttccattatg
     8101 ttgatcagtt tccaagtaaa agaaagacaa catcaggaat cgcttcaact gtatcaaata
     8161 aatgaagaat attaccatca tctagaggaa ttcaaacatg attataaaag tctactgttt
     8221 tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt tggaattttt agaaatatta
     8281 gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac aaatattgta
     8341 tccccagctg tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc ggagaaaatc
     8401 ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc cgattgatct ggttgttttt
     8461 attcgctgtt tatctatttt tatttctaac gcattcgatc atcgtgtagc cgatcagagt
     8521 ccgattacga tcaatctgct ggaagaaaaa gagggatcac gttttagtat agcgaacaag
     8581 gcaaatccaa cgaatttgac gctatcggag atggtcaaaa gaggaaaaac ctctaaaaaa
     8641 aatggcgggt taggtctata cagtgcgaaa aaaatgttgg atgtttacga aaatgcagaa
     8701 ctaaaaatcg aattttcaaa acagaatcac tatttttttg tagaaatgta tctggcatct
     8761 agaaagagca aatcagccta ctgacttact gtcaaaggat aagtttgagg aaagtctaca
     8821 cggagataga gacaacaaca cagcatattc aaaagaaaca gaagcgtgat gaccgttgtc
     8881 acccttctgt ttcttttgct atgtagttaa acaaagtagt tgacacttcc gctacttagt
     8941 attagtacga aagtatactt catgatacta agtagataag aagtgaaaag cggaacccac
     9001 gatcgtcaaa atgctaactg ctttgcttac accagataaa gggaagagca atatcgctta
     9061 ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct gacccagcag
     9121 ttaagagagt tagaacaaga tgaaagtatc catcgaaaag cctataacca agtcccgcct
     9181 aaagtagagt actcgctcac cgaagaagga aaatcattaa gagagattct agtagcgttg
     9241 tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct caagggaaga agacacgcta
     9301 ctgcatacac atggtggcta cctaaattat tgattttcta tgacatttat tttaaataaa
     9361 agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa tggtatgatt
     9421 aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa aaagtcaacc
     9481 agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg gctatctcta
     9541 tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg gaagaagtaa
     9601 aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc tattcataca
     9661 tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa ttgatcaaaa
     9721 ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac acagagttgt
     9781 agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat gagtctggag
     9841 ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat cctcttgttc
     9901 ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga ttattttgtc
     9961 agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct gaaaaagaat
    10021 aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac agaaggggtc
    10081 atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc agtcccttca
    10141 gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact ttacggagca
    10201 ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc aagccatcgt
    10261 gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg tgccaagatc
    10321 tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga aaaagttgct
    10381 cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac actaattact
    10441 tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga aagtgttaca
    10501 tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac aaaaacattt
    10561 taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat gaatcgataa
    10621 ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct gatttggttg
    10681 ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg cttgcataaa
    10741 taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt acggcaggca
    10801 aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt tttcttgaga
    10861 gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat ttcgaaaaat
    10921 ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa aaagattggg
    10981 gacgagattt cgccagaagc ggtccaaaaa gaatttgttt taagagattc ttttgctaaa
    11041 aaccaattcc agcagctgat ggctgatatt ccggcagagg agatcgaatg ggtcaaacaa
    11101 gtagtcgaac tggctgaaga taaactaaaa gttgaatttt cgcctaatat ttatctgaca
    11161 ttgaccgatc atcttcatta tgcgatcaca cgagcaaaag aaaatatcca actgcctaat
    11221 ccattattat ttgaaacaaa aaaattttat ccgaaagaat atcaagcagc gaaagaggct
    11281 cttttgttcg tgaaacaaaa gacgggtgtg gaactttctg aggatgaagc cggttttatt
    11341 gcctttcatt ttgtgaatag ccaacaagga aacgaagaca tgcaagtgac gatgaccgct
    11401 accacaatgg taagagatat actgagtatc atcagtaaat tcttcggcat cgtgttcgat
    11461 gaagaatctt tgaattatca gcgtatcatt actcatctgc agttttttgc ccagcgttat
    11521 ctcaaagggg aaggatctga cgagcaagat gaatttctct acgcattagt tcaaggaaag
    11581 tatccaaaag cgtttcattg tgtcgaacgg atcaatgagt atcttcgaca aacgcaaaaa
    11641 caggaaatgg ggatcgcaga gcagatttat ttaacgattc atattcaaag agtagtaagt
    11701 gagaaaaaaa gcatccaatc ataaggattg ttactgtatg gcaggcaaaa cctggattga
    11761 aaaaataggt tcacgaatgt ttgtgactgt ttttttcaat ctgggttttt attttttaaa
    11821 taaaaaagaa agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg tgtatgggaa
    11881 gcggtaggag gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg gttgcgattc
    11941 agattaaaag atgaatcttt agcagatacg caaaagttaa aagaagaccc ggacgtgatc
    12001 caagtcgtac aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt agcagatgtt
    12061 tatcaatcga tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa agaagagtcc
    12121 aaaaatattt tgaatcgtct gatcgatatt atctctagta tatttacacc ttttctaggg
    12181 gcgatggcag ccgccggggt attgaaagga ttcttatcat tggcaacggt gatgggctgg
    12241 ctgacagctg atagcggtac ttatcaaata ttgtttgctg cagcagatgg tgtctttacc
    12301 ttcttacctg tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa ccagttttta
    12361 gcagtagcaa ttgctatggc acttgtgtat ccagctatca cagcagttgc tggtgcggga
    12421 gaagctatca gtttctttgg gatcccagtg attctttcac catctggtta tacttcttct
    12481 gtcataccaa tcattctagc cgtatgggtt caaagtaaat tagagccatt cgtaaaaaaa
    12541 gtgattccgc aatttctgca aatgatttta gttccgcttg tcgttttagt agtgatggtt
    12601 ccattaacat ttcttgcttt aggaccaatc gggacggttg ccggtaatgc tttaggcggt
    12661 ctcttcaact cgatttatgg atttagtcca atcgtagcag gtctaatcat gggaagttta
    12721 tggcaagtat tcgtgatgtt cggcatgcat tggggattcg taccgatcat gtttttgaat
    12781 atcgaacaat atgggtttga tgtattgatg ccaatgcttc ttccagccat cttggcacaa
    12841 ggtggggctg ctttagctgt agcattgcga acaaaagata cgaaacttcg agcattaggg
    12901 atttcttcta cagtgacttc gttatttgga atcactgaac caactgtgta tggtgtgaca
    12961 ttacctttga agaaaccatt cattgccgct tgtatttctg gaggtattgg tggagctatt
    13021 atcggatttt ccggagtaaa agctttctcc agcagtttgg tcagcttatt gacgattccg
    13081 actttcatta atacggtgga tggtgtagag tcaaatgtga cagtagcagt aatagctaca
    13141 ggaattgctt ttgttcttgc attcgtaggt acattgattt taggatttga cgaacaaaca
    13201 caagacaatc aattagaaaa caaacatgcg aatgcaggaa aaccgattac ttcagcacgg
    13261 cataccttaa aaagtccttt aacgggtaaa gtactgccat tgagtgaagt accagaccaa
    13321 gttttttctt ctggcgtgat gggaaaaggt atcgctattg atccagaagt aggagaacta
    13381 gttgcacctg cagacggaga gatcacgact atttttccaa ctggacatgc tgtagggatc
    13441 acaacgactg acggagcgga gattttgatt catatcggga tggatacagt agaattaaat
    13501 gggaatggct ttgagatctt agtcaaacaa ggggatctcg tcaaagcagg agatttattg
    13561 attcgttttg acatcgaagc aattagagca gctggctata gcgtaatcac tccagttgtc
    13621 attacgaata ctgatgcatt tgccgatata cttgaattag accaaaaaga gattattgca
    13681 aacgaagatg ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat cacgtttttc
    13741 agaaaaattt ttatggggtg gagcaactgc tgccaaatca attagaaggg gcttatgatc
    13801 gagacggaaa aggactatcc gttgcagatg ctatgcctgg aggtaagcag cgctttgcga
    13861 ttatcggcag tgaagaattt gactggacga ttgatcaaga aaaatatatt tacccgaatc
    13921 atcgaggaat cgatcattac gatcgtttca aagaagatct tgccttgttt gcggaaatgg
    13981 gattcaaatg ttatcgtttt tcgattgcct ggacacgaat ttttccaaat ggagacgaag
    14041 gtactcctaa cgaagcggga ttagaatttt atgatcagct gattgatgag tgcttaaaat
    14101 atgatattga acctgtgatc acgatttctc attacgaaat gcctcttcat ttggcaaaag
    14161 aatacggtgg ctggaaaaat cgaaaactga tcgacttcta tgaacgattt gctcagacag
    14221 tattagagag atacagcagt aaagtcaaat attggatgac tttcaatgaa atcaattcgg
    14281 cctttcactt tccagcttta agtcaaggat tagtaaaaag caatggagca ggagagtacc
    14341 aaaatatttt ccaagcatgg cataatcaat tcgttgcaag tagcaaagcc gtaaaaatcg
    14401 gccacgaatt gcgttcggat atccaaatcg gctgcatgat tatctatgca acaacttaca
    14461 gcatcgatgc caatccagtc aatcaggcag caacaatgat ccaaaaccaa gaattcaatt
    14521 ttttctgcac ggatgtccaa gtacgtggag aataccctgc atacacagcg cgtacccata
    14581 aaaaatatgg agtcgatccg gaaaaattag agcaaacgga aaaagacttc cagttgctaa
    14641 aagaatatcc agtggactac atcggattta gttattatat gtctacggca atcaatgaga
    14701 ctgatccaca agcagcaact tcagaaggga acttgctggg aggagtaaaa aatccgtttc
    14761 ttgaggcaag tgaatggggc tggcagatcg atccagaagg gctgagaatc gcattgaatg
    14821 aattatacaa tcgttaccag aaaccattat tcattgttga aaacggtctt ggagccattg
    14881 atcatatgga agaggatggc acgattattg atgattatcg gatcgattat ttacgtcgtc
    14941 atatcgaagc gatggctaat gcagtagcag acggtgtaga tttgatgggc tatacgcctt
    15001 ggggatgtat cgatcttgtc agtgcatcaa caggagaaat gagtaaacgt tacggcttta
    15061 tctatgtgga tctagacgat gaaggaaatg ggacactgaa tcgatcaaag aaaaaatctt
    15121 ttgattggta taaagaagta attgcctcaa atggcgtaaa cttgtaatta aagcagctga
    15181 atgaaaaaga acacacttct cacatgtgat ttgtgttctt tttttatttt ttatgtgcta
    15241 ttcttttagt aatagtaaag caaagggtga cgttatgaaa aggacacgtg tactatactt
    15301 ggaagtcgca gataaaatta aagaagatat tttctcagga aaatatccag tcggttcgat
    15361 gttgccgaca gaaagtgaat tagaagaact gtttcaagtc agcaaaatca ctgtccgaaa
    15421 ggcaatcgaa ctgctggcat cagatgaata tgtcgcaaag aaaagtggtc gtgggacgac
    15481 ggttctaagc aatcgtcctt acaatcgatt atccaaagcg gcaagcttta cgcaaatctt
    15541 ggagaactcc cgatatgaag tccggaaaga aacgctggat ttgcaattgg tccatataaa
    15601 gaaggaagat cctttgtttt cctttttcgg tgagcaagct gtttgtttta agcgcttata
    15661 ttatttgaac aatcagcctt atatttattt tgttcattat cttccacaag aaatgatgca
    15721 attcagcaga gaagaatttg gcaaagaatc tttgtatcgt ttgctgacga aaaaaggata
    15781 tactattgac tcattttctg atgatttctc ggcagtattt ttaacagaag aagaacaagg
    15841 aattctgaaa acagacgcac agatcggcat caaacggata cggaaatcgt ctacgcaaat
    15901 tggggaaatg attgaatatt cggaggcggt ctaccatacg gagctgcatc cttaccatat
    15961 tgattatgaa acataaataa aaaaagaggt tgtgaaaaag ctgccctgca tcaagtaata
    16021 agaacagcca aacaaaaata gcttctcata tttttcgttt ggttaggctt attaccgcag
    16081 atacacggct gtttctggat taccatttat tctggataag ggggtgtggc acgattttca
    16141 gaaggaatct atgaaccaat cactgtattg attttagctt cgatggtaac cccattcggc
    16201 ttgacgatcg cttatttctt agggaaaatc attcgtaaaa atatactcaa tcgtcaagaa
    16261 atcgatacat tgaagaccgc ttttcctatg gggatctgtc agatcacaga gggatgtttc
    16321 cctatcgtat tgaacgactt ggcacgcaat gtcatagcaa cgggagtcgg tgggacagta
    16381 ggtggcggat taagcatgtt ttggggcgca gatagccgta ttccggcatc gggaatgttt
    16441 gcagtcgcta cgatgaccag accatgggca ttcattggtg cattgttagc tggttctttt
    16501 gtcacaggaa taaccatttt attattgaaa aaaccagtag atccgaatgc agaaatcatc
    16561 caagaagaaa aagaggaaga agacatatct tgggatgatt tgacaattag ttaggagagg
    16621 cagaatggat tacaagaaaa aagtacaata gatgtacctt gagacaggaa tggtatgaaa
    16681 gaatcagagt tagaaagaat cgaatataca gattttggtt taggagcgat cgaaactgtc
    16741 cagagcatcg gcaccctcct tgaaatgacg atgaaggaaa aatgagaacc tttcgttgtc
    16801 gaaagggtct ggtatacctt atgtcgaagg aaaggaaaca aaaaaacttt cctgtactat
    16861 tccaaaaagg aaagaaagtt attatacagt aaaaaaagaa attattcttc atgctagaga
    16921 tcagtacacg ttcgagccca atatcaaaca ctagttccag gcaggcgcaa caggtgcggt
    16981 tatcactgaa ttttcttcca gcagtgacga ttcaagtgat atttttacaa atccagctat
    17041 ttatcgttaa caaaaagatt tttaggagaa gctgatgaac aaaaatgatc aaattttgat
    17101 cgtttagttc gtcggctttt ttatttagag aaacatcatt tatcagtgaa acataacacg
    17161 agaacaaaat gattttttcg ttaataaaag aaacaagtat ttgttgttta aaataataca
    17221 cgttattggc a
//
LOCUS       DAHUWM010000041        17217 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHUWM010000041 DAHUWM010000000
VERSION     DAHUWM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.041"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            3..371
                     /locus_tag="NK788_000958"
     CDS             3..371
                     /locus_tag="NK788_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCH6769302.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="NK788_000959"
     CDS             551..1492
                     /locus_tag="NK788_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCH6769303.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="NK788_000960"
     CDS             1561..2292
                     /locus_tag="NK788_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCH6769304.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="NK788_000961"
     CDS             2436..4616
                     /locus_tag="NK788_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCH6769305.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="NK788_000962"
     CDS             4636..5118
                     /locus_tag="NK788_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCH6769306.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="NK788_000963"
     CDS             5134..5895
                     /locus_tag="NK788_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCH6769307.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="NK788_000964"
     CDS             6026..7360
                     /locus_tag="NK788_000964"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCH6769308.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="NK788_000965"
     CDS             7667..8152
                     /locus_tag="NK788_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCH6769309.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="NK788_000966"
     CDS             8145..8828
                     /locus_tag="NK788_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCH6769310.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="NK788_000967"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="NK788_000967"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCH6769311.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="NK788_000968"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="NK788_000968"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCH6769312.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /locus_tag="NK788_000969"
     CDS             12444..13073
                     /locus_tag="NK788_000969"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCH6769313.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="NK788_000970"
     CDS             13125..13457
                     /locus_tag="NK788_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCH6769314.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="NK788_000971"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="NK788_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCH6769315.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="NK788_000972"
     CDS             14283..15656
                     /locus_tag="NK788_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCH6769316.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="NK788_000973"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="NK788_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCH6769317.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="NK788_000974"
     CDS             16711..17151
                     /locus_tag="NK788_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCH6769318.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5873 a         3008 c         3792 g         4544 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaagg
//
LOCUS       DAHUWM010000042        17164 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHUWM010000042 DAHUWM010000000
VERSION     DAHUWM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.042"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(264..3938)
                     /gene="rpoC"
                     /locus_tag="NK788_000975"
     CDS             complement(264..3938)
                     /gene="rpoC"
                     /locus_tag="NK788_000975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCH6769319.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3996..7613)
                     /gene="rpoB"
                     /locus_tag="NK788_000976"
     CDS             complement(3996..7613)
                     /gene="rpoB"
                     /locus_tag="NK788_000976"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCH6769320.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKWVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8341..9309
                     /locus_tag="NK788_000977"
     CDS             8341..9309
                     /locus_tag="NK788_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6769321.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9327..10232
                     /locus_tag="NK788_000978"
     CDS             9327..10232
                     /locus_tag="NK788_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769322.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10229..11035
                     /locus_tag="NK788_000979"
     CDS             10229..11035
                     /locus_tag="NK788_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6769323.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11171..12160
                     /locus_tag="NK788_000980"
     CDS             11171..12160
                     /locus_tag="NK788_000980"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCH6769324.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12477..13001)
                     /locus_tag="NK788_000981"
     CDS             complement(12477..13001)
                     /locus_tag="NK788_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCH6769325.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13288..14985
                     /gene="scm"
                     /locus_tag="NK788_000982"
     CDS             13288..14985
                     /gene="scm"
                     /locus_tag="NK788_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCH6769326.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15140..16126)
                     /locus_tag="NK788_000983"
     CDS             complement(15140..16126)
                     /locus_tag="NK788_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCH6769327.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16149..16715)
                     /locus_tag="NK788_000984"
     CDS             complement(16149..16715)
                     /locus_tag="NK788_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCH6769328.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16729..17145)
                     /locus_tag="NK788_000985"
     CDS             complement(16729..17145)
                     /locus_tag="NK788_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCH6769329.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
BASE COUNT         4927 a         3703 c         3334 g         5200 t
ORIGIN      
        1 aggaattatt caataatagc tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa
       61 tttattttgc attcaaaaaa tgatctatct ttacttttgt tgattgactt ctcttggaag
      121 atgatcgttc cgatacctta attggatacg aaccgatgtt gttaagatcc tttcccactt
      181 ttcttctagt tagacaaatt attttagaca aaccaaaaaa gaggttgtga caataatttg
      241 tcacaacctc ttttttaaaa tatctatttt gtttgattgt tcattgcgtc ttctgcagcc
      301 atttgcgctt caatatctga aatactgtag acattttcgc ttgctacacc gacttctttt
      361 ggttccatgt cacgataacg ttgcatacca gtaccagctg ggatgatctt acctataata
      421 acattttctt tcaaaccaag aagaggatct ttcttaccgc ggatcgcagc atccgtcaag
      481 acacgagttg tttcttggaa tgatgcagca gataagaaac tatttgtttc taatgatgct
      541 tttgtgatac ctagtaagac aggtcgagaa gttgcaggaa cgcctcctga gactagcgta
      601 ttgtagtttt gatctttaaa gtctgcgatg tctaataatg tacctggtaa gatatctgta
      661 tcacctggat ccattacacg aactttacgc agcatttgac gaaccattac ttcgatatgc
      721 ttgtcgccga tttctacccc ttgcatacgg taaacacgtt gtacttcacg aagcaagtag
      781 ttttcaacag ataatacatc gcgaacttgc aacaattgtt ttggatcgat tgatccttct
      841 gtcaatggtg caccacggtg gatcatatcg ccttctgcta ctttcatacg tgctgtataa
      901 ggaacagtat acgtacgtgt atctgttttt cctttgattg ttacttcttt ggtacgtgtt
      961 gcaggatctt ctgagatatc gatgacatcc cctgttactt ctgtgatcac tgcttgccct
     1021 ttcggattac gtgcttcaaa gatttcttgg acacgaggaa gaccttgagt gatatcgtct
     1081 ccggcaaccc cacctgtatg gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt
     1141 gattgagcgg cgatcgtacc aactgcttct ccgacttcaa cgtcagagcc agttgcaagg
     1201 ttgcgtccgt aacaatgttt gcagacacca tgttttgtat tacatgtgaa gacagaacgg
     1261 attgtcactg tttcgacacc tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat
     1321 tggtttcctg caatgatcaa ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa
     1381 cgtcccacta aacgatcttc taatgattcg atgatttcat ttccctcgcg gatggcttgg
     1441 atttcaagac ctcggtcagt accgcaatct tcttcacgga tgattacatc ttgggccacg
     1501 tcaaccagac gacgagtcaa gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc
     1561 cctttacgag ccccgtgagt tgagataaac atttccaaga cggatagtcc ttcacggaag
     1621 ttcgaaatga ttggcaattc catgattcga ccattaggag cggccatcaa accacgcatt
     1681 ccggcaagct gtgtaaagtt ggaaatgtta ccacgagctc cagaatcaga catcatgaag
     1741 attggatttt tcgcttccaa accttccatc agtttttgtt ggatgctgtc ttttgcagca
     1801 ttccaaacag caatgacacg ttcataacgt tcgtcatctg tgatcagacc acgacggaat
     1861 tgttttgtaa tagtttcaac ttgtttatgg gcattttcaa tgatttcttg tttttcattc
     1921 agtacactga tgtcagcgat ccctactgtg ataccggcat gtgtcgaatg tttgtaccct
     1981 aagtctttca tacggtcaag catcttagaa gtttctgtta ctttgaatcg tttgaagact
     2041 tctgcgatga tgtttcctaa gtttttcttc ttgaacggta atactaattc ttgctctttg
     2101 atatgtgcag gaatatcagt accagcttca acaaagtatt tgtccggtgt ttggacggtc
     2161 aagttgaagt ctgttggttc attcagataa gggaattcag gaggcatgat ctcgttgaag
     2221 atgatcttac caactgttgt gatcatcgta cgttcttttt gccattcagt aaatggtttt
     2281 tcacctaatg agttagggtt cacaccgata cgtgaatgta aatgaacata tccgttgcgc
     2341 catgcgataa cggcttcgtt ttgatctctg aagaccattc cttcgccttc gcgtccttct
     2401 tcttccattg tcaagtagta gttacctaga accatatctt gagaaggagt aacaactggt
     2461 ttaccatctt tagggttcaa gatgttttga gcagctaaca tcaacatacg tgcttctgct
     2521 tgtgcttctt cattcaatgg aacgtggaca gccatttggt ctccatcgaa atcggcatta
     2581 taagcttcac atacaagtgg atgcaaacgg attgcgcgtc cttgtactaa aactggttcg
     2641 aatgcttgga taccaagtcg gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc
     2701 ttgattacgt cttctaaaac atcccatact tcgtcttctt gacgttcgat tttacgtttc
     2761 gcatttttga tgtttgaagc tagttcgcgt tgaacaagct cacgcatcac gaatggtttg
     2821 aataattcaa tggccatttc ttttggaagt ccacattggt acatcttcaa gaaaggtcca
     2881 acaacgataa cggaacgacc tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa
     2941 cgaccttgtt tccctttcaa catatgagaa agagatttca atgggcggtt acctggtcca
     3001 gtaactggac gtccgcgacg accgttatcg atcaatgcat ctactgcttc ttgcagcata
     3061 cgtttttcat tttgaacgat gattccaggt gcattcaagt ctaacaaacg tttcaaacgg
     3121 ttattacggt tgattacacg acggtaaaga tcattcaagt cacttgttgc aaaacgtcca
     3181 ccttctaatt gaaccattgg gcgtaaatct ggtggaataa cagggataac gtccataacc
     3241 atccagcttg gttgatttcc tgaagcgcgg aatgcttcta aaatatccaa acggcggatt
     3301 gcacgtgtac gtttttgtcc ttgtgcagat ttcaattctt ctttcaattc ggctgcttct
     3361 gcatctagat caacattatc aagtaattgt ttaatcgctt ctgcacccat agcagcttgg
     3421 aattcttgtc catattgttc acgtttttcg cgatattcgc gttctgtcaa caattgtttt
     3481 ttctctaaat tcgtatttcc tggttcgatg actacatatg aagcaaaata aatgatttct
     3541 tccaaagcgc gtgggctcat gtctaataca agacccatac gtgaaggaat ccctttaaag
     3601 taccagatat gtgaaacagg agctgctaat tcaatatgtc ccatgcgttc acggcgaact
     3661 ttcgaacgag taacttccac tccgcatcga tcacaaacga ttcctttata acggatacgt
     3721 ttgtattttc cgcaggcaca ttcccagtct tttgtaggac caaaaatccg ctcacagaac
     3781 aacccttcac gttcaggttt taaggtacga tagttaatcg tctcaggttt ttttacttca
     3841 ccgtaagacc agcttctgat tttttcggga gaagccaaac ctatttgcat actttcgaat
     3901 ttatttacat cgatcaaaag gggttccctc catttcattt aatggcaaac cgcaagacgg
     3961 ctgatcttcg accgccttgc agctcataga ccggattagt ctaaattatc ttctgcggtt
     4021 tcaaagtttt ggatgacatc gtctttttca tcttccactt gttcagcagc tttcttttct
     4081 aattctttgg cagtttgctg ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg
     4141 tcgtcttcat cgtccatgtc gcgaagttcg atctctgttt cttcgatatc caagacacgc
     4201 atgtccagac ctagtgattg caattctttc accaatacgc ggaatgattc cggtacacct
     4261 ggttttggaa ttggttcacc cttgacgatt gcttcgtatg ttttcacacg tccgactacg
     4321 tcatctgatt tgtatgtcaa gatttcttgt aaagtataag ccgcaccgta agcttccagt
     4381 gcccatactt ccatttcacc aaaacgttgt ccaccaaatt gagctttacc tcccaatggt
     4441 tgttgtgtaa ccaatgagta aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg
     4501 tgagcaagtt tgatcatgta catcacaccg acagacacac gtccatcaaa tggttcacct
     4561 gtacgtccgt cgtacagaac agttttcgca tcgctggcca taccggcttc acgaactgtt
     4621 tcccatacgt cttcatcact tgcaccatca aatactggcg ttgctacgtg gatacctaac
     4681 tgacgtgcag ccatacccaa gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc
     4741 acccctaaag gattcagcat gatatcgatt ggtgttccat caggtaggaa tggcatatct
     4801 tcttccggca taatacggga tacaacccct ttatttccgt gacgaccggc cattttatcc
     4861 ccttcatgga ttttacgttt ttgaacgata taaacacgaa ctaacatgtt tacgcctggt
     4921 gacaattcat ctccagcttc acgagtaaag attttcacat cgtgaacgat accgccgccg
     4981 ccatgtggta cacgtaatga tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca
     5041 tgcaatagac gttcttctgc agataattct gttacccatt ttggcgtgac tttccctaca
     5101 agcaaatcgc catcttggac ttctgcacca atacggatga tacccatttc gtctaaatct
     5161 tttaatgcgt cttccccgac attagggatt tcgcgtgtga tttcttcagg tcctaatttt
     5221 gtatcgcggg cttctgattc atattcttca atatggatag aagtgtatac gtcatctttt
     5281 accaaacgac ggctcatgat gatcgcatct tcatagttgt acccttccca agtcatgaaa
     5341 ccaactaaaa cgttttggcc tagagccatt tcgccttgtt ccatagatgg tccgtctgcc
     5401 agagtatccc ctttttcgac tttctcaccc aaatgaacga ttggacgttg gttgtagctt
     5461 gttccggagt ttgaacgacg gaattttgtt acatcgtatt tatctaatgc accgttgtcg
     5521 cggcgtacgc ggatttcaga agcatcaacg tattcaacga caccgtcatg tttacataac
     5581 aaagcagctc ctgagtcatg cgctgatttg tattccatac cagtaccaac ccatggtgct
     5641 tgaggattga tcaacggcac tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg
     5701 gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac ttgtttaggt
     5761 gatacgtcca tgtagtctac tttatcaata gatacttcta agttttcact ttgagcacga
     5821 gccataacaa cgtcttgagc aaatgttcca tcttcattca atgggctgtt tgcttgggct
     5881 acgatgtaat ggtcttcgat atcagctgtc aagtagtcga tttggtcagt aacacgacct
     5941 gtttcacggt ctacacgacg ataaggtgtt tcgataaatc cgaatttatt tactttcgca
     6001 taactggaca aactattgat caacccgatg tttgggcctt caggagtttc gatcggacac
     6061 atacggccat agtgggagta gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc
     6121 aatccgccag gtcctaaggc tgaaagacga cgtttgtgtg tcaattcgcc aagagggttt
     6181 gtttgatcca tgaactgtga caactgagaa gaaccaaaga attctttgat acttgccaca
     6241 actggacgga tattgatcaa ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt
     6301 ctttcacgaa ccacacgttc catacgtgct agcccgatac ggaattggtt ttgtaaaagt
     6361 tcaccgactg aacggatacg acgatttcct aagtggtcga tatcatcgac attgccgata
     6421 ccttccatca agttcaagaa atagctcatt gaagcaatga tgtcggctgg acgaaccgtt
     6481 tttacagctg cttctggata gccgtttccg atcacgttta cttcacgttc agggtctctt
     6541 ggtgaaaata ctttgatgac ttggacagtc attggatctg tgaccacacc gtcttctgat
     6601 ggatagtaag tcactgaatt taggccatta tcaatgaaag gagctagtgt ttccatgact
     6661 tggtgagtca gaactgttcc tttttcaacg atgatctcgc ctgtttctgg atcaactaat
     6721 gtttcagcta atgtcagatt caatagacgt gttttcaaat ctaatttttt gtttactttg
     6781 taacgaccaa cgtttgctaa gtcgtaacgt tttggatcga agaaacgagc attcagcaag
     6841 ttacgtgagc tgtctgctgt ttttggttcg cctggacgca aacgttcgta aacgtctttc
     6901 aaaccttctt ctgttcttga gtcgcttgcg tttttatgca agtctttttc gactgtattg
     6961 cgtaatgttt cgctgtcgcc gaagatttca aagatcgtat catcggaacc aaatcctaac
     7021 gcacgaacta atacggttaa aggaatctta cgtgtacggt cgatacgtac ataagagatg
     7081 tcttttgcat cagtttccat ttctagccaa gctccacggt tagggataac agtagaaccg
     7141 aatccttctt ttccgttttt gtcgactttt ccatggaaat agacacctgg tgaacggact
     7201 aactgagaaa cgattactcg ctctgccccg ttgatgatga atgttccttg ttctgtcatt
     7261 aatgggaaat caccgaagaa tacttcttgt gctttgattt cgccagtctc gcggtttgtc
     7321 aaacgcaatg ttacatgtaa tggtgcagag tagtttgcat catgcgcacg agcttctgct
     7381 actgtatact ttggttcttt caattcataa tctacaaatt ccaaagatag attgccattg
     7441 aagtcatcga ttggcaaaat gtcttcaaac atttctctaa gtccctcatc taagaaccat
     7501 tggtaagagt ctgtttgaat ttcgatcaaa tttggtaatt ccaacacttc actgattctt
     7561 gcgaaacttc tacgttcgcg atgttttccg tattttacta cgtgtccagc caagctcttc
     7621 acccctctaa aatgtcattc aaaaagacta cccgatcaat tgttacaata atatttcaaa
     7681 tattattttt aggtaacaac agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa
     7741 tagaacattc tcttgaaacc ttacttcgtg agcttctact tttgtttcct tatattaaag
     7801 ccgaaacgga caatatttcg tttctgcctt ttcttcatca gatagttttt gcaacttcat
     7861 atactacact tattttctat aaaagtcaac acttcctggc gtttcttctg ctttttcgca
     7921 acattttctt aatgttttca attttatgtt ttcaattatc tgacaattct taattgacag
     7981 acctttttat ctcggttata ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac
     8041 caacatcaac caaatcgcac caacacaact gaataacttt tactttagct actttagata
     8101 ggtttgtatt catcatgatg gatacaaacg ttaaacgaca gtttgtatct atgatggcta
     8161 aagaactttg tattgcagca aaaagccacg tagacaaaat gctaagtggc tgagcagtga
     8221 aaatacaaag taaaagtatc aagcattcta ctttttcaat ttattttccg acttttcaat
     8281 ccatttggac attttgtctg aatggatttt ttgtttccca aaattaagga ggaaaaagaa
     8341 atgaaaaaaa atataatcat cggattaggc gctgccctgc tactagcttt aggaggttgc
     8401 gcacaaaaag acaattccgc agaggcatca gatggacaaa aaacaattgg cgtattgcaa
     8461 cttgtacaac ataactcgtt agatgctgcc tataaaggat tcaaagaagg attagcagaa
     8521 gaagggtata aagaaggcga taatctaaca atcgattatc aaaatgccca aaacaatcaa
     8581 gacaacctga aaagtatgag tgagaaactc gtcaaagaag atcctgattt gctacttgga
     8641 atcgccactc cagcagcaca atctttatta aatgaaacaa ccactatccc gatgactgtc
     8701 acagcggtca cagacttgga acaagcccat ttagtcacat ccaatgaaaa acctggcggc
     8761 aatgtgactg gcacgacaga tatggtgcca atcgaaaaac aaatcggctt actgttaaat
     8821 atcgtgccag acgccaaaac aatcggcatc atgtataaca acggtgaagc aaactctaag
     8881 atccaagctg atttggcaga aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg
     8941 acagcaaatt ccacaaatga tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat
     9001 ggaatctata ttcctactga taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc
     9061 gcaaaagaaa caaaaacacc tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt
     9121 ctagcaacag taggaatcga ctacgaaagc ttgggcaaac aaacagggat catggcagca
     9181 aaaatcttag acggcaagaa tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa
     9241 ctgtatgtca ataaagacat ggccaaagca ttaggcatca atccagacag catcaaagaa
     9301 gctgaataaa acacaggagg attctcatga caaatttgct ctctcttttc ttatcatctg
     9361 catcgcaagg ggtcttatgg gccctacttg cgattggtgt atacgtcact ttccgtatct
     9421 tggacattgc cgacttgact gctgaaggaa gctttccttt aggtgcaggg atcgcggcag
     9481 taagtattac aaatggctat catccattga cagcttgtct tctaggcttt ttagggggtg
     9541 cggcagcagg gcttgtttcc ggactgcttc acacaaaatt gaaaattccc gcccttcttg
     9601 cagggatcat caccatgact ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga
     9661 acatctcttt attgggtcag ccaacgatct ttcattgggt cgagtcattc ggcataacaa
     9721 aagacaacgc tgtactgatc gtcggtctgc tttttgcttt gatcgttgta tgtttacttg
     9781 ttttgttctt acgtacagaa acaggacttg ctattcgtgc aactggcgat aatctggcaa
     9841 tgagcgaagc aaacgggatc aacacagata atatgaagat catcggttat atgatttcta
     9901 atggatgcat tgctctttct ggctccttac ttgcgcaaaa caacggcttt gctgatttaa
     9961 attctggtat tggaacgatc gtaattggac ttgcttctat tatcatcgca gaagttttgt
    10021 tacgtaacca gccactattt ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc
    10081 gttttatttt agcgttagtc ttcgaactga atgttgaacc aagtgattcg aaacttgctt
    10141 ctgctttcgt gctagtgatc tgtctttctt tcccgcagat ccagcaaaaa ttccatttac
    10201 ctcaaaaaaa gaaaggcggt agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca
    10261 tcataccttt gaaaaaggaa cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc
    10321 cttagatccc ggcgacttcg tgacgatcat cggtggaaat ggggctggta aatctacttt
    10381 gctgaacagc atcgccggta cattttctgt cgatcagggt caaattttat tgaatggaaa
    10441 aaatatcacg aaaaaatctg ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga
    10501 tcctaaacta gggacagccg ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa
    10561 acgaggaaaa aaacgcggct tcttccgtgg tgtcaaacca caagaccgaa catttttcaa
    10621 agaccagctg gttcggctaa atttggggtt ggaaaatcgt ttatcctcag aaatcggttt
    10681 gctgtctgga ggacaacgtc aagctattac gttattgatg gccacattac agcgtccaga
    10741 actgatctta ttagatgaac acactgcagc gcttgaccct gctacttcca aaacggtgat
    10801 ggaattgaca gaaaagctga tcactgagca gaatctcact gcttttatgg tcactcatga
    10861 catggaagac gcgatccgct atgggagtcg actgatcatg ctgcatcaag gaaggatcgt
    10921 cgtagatatc cagcaagagg aaaaacagca gctcacagtg ccccaattat tagcgttgtt
    10981 caaacaaaac agcggcacgg cattaaaaga cgatcaagta cttttatctt cataatcaga
    11041 aagaggatgg gacaaaaaat gaacgcccac cctcttcttt tttctctttc cttctttcgt
    11101 ttttgcttta tgtatcaacc gttgtttctt cgaaaactat tttgttctct ccttcttcct
    11161 gatagaatgg atgggaaagg agcttgatgc catgacaacg aaagacaagg tacttcaatt
    11221 attgaaaaag acaccagatt ttctttccgg agaaaagttg gcacagcaac tggaagtttc
    11281 ccggacaagt atttggaaag ccatcaaaga attggaaaag gcaggctatc gttttgagca
    11341 cgggcctaca gggtatcgtt acctgccctc tgacgtacta gatgcaaaag aaatccagca
    11401 agagctacgg tcgtttattc ctgatttgaa agtacaaacg atgttgactt ctgaatctac
    11461 gatgaaagat gccaaactgg cagcagctca aggagattct gtttcgaagt tatttgttgc
    11521 tgatacacaa gaacaagctc gtggacgttt tggacggcct tttttcgctg aacctggacg
    11581 aggaatctac atgagtctct tgctccatcc agacaaacgg tttgatgaat tgcctcaata
    11641 tacgatcatc gcagccagtg cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc
    11701 ttcgatcaaa tgggtcaatg atatctacat ggatggaaaa aaagtctgtg ggattttatc
    11761 tgaagcagtc agtgatatgg aaagcggcag gatcagtcat atcatcattg ggatcgggct
    11821 taatttttct gttcctcaaa atcattttcc agctgatctc caagaaaaag ccatctctgt
    11881 ctttcccgac ggacaagcta gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga
    11941 attgttcact tatctttctc acccagaaaa agcgttggac ttctataaag aaaaatcatt
    12001 cgtattggga aaaaccgtgt cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa
    12061 agaaatcggt gatcatggcg aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc
    12121 ttctggcgaa atcagtctca catctatccg tacgcaataa ttatcagatt tcacaaaagg
    12181 acacctgatc tcatacagtg tccttttttc tgaattctct tcaaaagact ctccttattc
    12241 tatcagctga cagtttctct atcctctaga aaaaggtatt ctaaaaatat ctataaagca
    12301 tggtacactt tctttaaatg aatcaatgca aaaaacaaga gagtcatcag tttacacgat
    12361 caaaagagat agcaggccgc ttcctttttc aatcttatag atagaccatg ggtaacgata
    12421 aaaacaaaaa agaacttgcg acttattttg ccgtaagttc ttttttagct agtgccttaa
    12481 tctcttaatc ccaacaaacg tctcgcatag atcacctgac cagagtgcat agcagcatca
    12541 tcaataatag aaacgagacg cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt
    12601 acttcatcta gctcggaagg ttccagcctt cttaagtaag aaatagcaaa atcagcagct
    12661 cctcgcaagt atcctagcag atcttctatt tgattagctt ggatctgctt tgcttgttcc
    12721 agactatgct tccaatcttg ttcgttatct gctatcgtca tcggaaaccg gctttcccag
    12781 tcttgactat gccaaattgg agaagttcct gctaaatcag caatctggaa atctaattct
    12841 cgagcggtat gccaagccag ccaagtcatt gatttgatct gcggggctct atcttccact
    12901 ggaaaacgat tggcctcctc aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat
    12961 gcccgatcca cactctcgca tagcatatct aaataatcca tttattttcc tcctttttat
    13021 ttatcctact tactttttcc taattctagg atacctactt atatgttact acacaccctt
    13081 attttttaca ctttaacagt caccatcatc aatagtttgg agattgaaag aaaaagatag
    13141 tgtcgaacta tcaattgaac aagaagcaac aatatgaacg tttcattaat attctttaaa
    13201 taaacaatga tttttcaaac gaacagttta agactaggag aaaaagagta taataggaaa
    13261 atgtttgaaa ggaaaggaag gggaaaaatg aaaaaaaggg caagtttact agtcctagtt
    13321 gctttgttgt tcaatatctt ttcgtatggt gtggccgttt cagcggaaac atacattcct
    13381 aaagaccctg ggaatattta taatgatcta actggtaact atggcttgtt aggaattgct
    13441 agtcaattcc acgtatttgc taaaaataag acaactgtga atgcacatac agacggaaat
    13501 atagcaacac atgaacttga tgcaaaaaat aattttggta cagagcttca tgaaggttta
    13561 ttgaaccaag aaataaatta tatacaaaaa actgattctt tcgctaattc ttcaggcata
    13621 cctacaggta gcacaagaat gaataaattt gtcgttggca gtaacgtgga tgtagcactt
    13681 caagatggtc atcctacagt caatggatcc aggcttgatc acttaactcc agatgatttt
    13741 tataaagatc gtgctgggca tgaatatatt gatttcgagc aggaatttgc taaattgaat
    13801 gtagcagcaa atgatcttgc aacgatcact ccagctaaaa catatactgc tgcagatttt
    13861 ccagacatga acaatcggac aatcgattta actgatttat cagataccgg gtttttattg
    13921 gtaaatatcg atgcagaagt gttaacgatg aatacgccac ttcaaatcat taatccaaat
    13981 gatcaagtag ttgtatttaa tgtcatcaac agttcttctg cattgaacgt acagtcacca
    14041 atcaaataca atgatcgttc aaatcatgaa acagaagatt tttcggatgc gaatatctct
    14101 tggaattttg gaaatgagat gactgatttg acaatcagtg ctccttttca aggaacaatc
    14161 cttgcgccta atgctactat ccgcattact caaaaccaag acggaacgat tataggaaca
    14221 aatgtgattt tagatgcagc taccaatcgt tgggatccta acgaaatatt tatcagcgac
    14281 aatggtacag atacgacaga cacaagcgac agtacagata catctaccac aagtgatagc
    14341 tcagattcat caacttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg
    14401 gatacatcag cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggac
    14461 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
    14521 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
    14581 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
    14641 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
    14701 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
    14761 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
    14821 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
    14881 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
    14941 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
    15001 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
    15061 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
    15121 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
    15181 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
    15241 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
    15301 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
    15361 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
    15421 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
    15481 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
    15541 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
    15601 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
    15661 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
    15721 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
    15781 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
    15841 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
    15901 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
    15961 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg atggcttgtt
    16021 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
    16081 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
    16141 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
    16201 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
    16261 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
    16321 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
    16381 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
    16441 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
    16501 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg
    16561 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
    16621 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
    16681 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
    16741 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
    16801 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
    16861 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
    16921 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
    16981 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
    17041 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
    17101 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
    17161 tcca
//
LOCUS       DAHUWM010000043        17000 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHUWM010000043 DAHUWM010000000
VERSION     DAHUWM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.043"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(511..3216)
                     /locus_tag="NK788_000986"
     CDS             complement(511..3216)
                     /locus_tag="NK788_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCH6769330.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3522..4469)
                     /locus_tag="NK788_000987"
     CDS             complement(3522..4469)
                     /locus_tag="NK788_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCH6769331.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4659..4928
                     /gene="rpsN"
                     /locus_tag="NK788_000988"
     CDS             4659..4928
                     /gene="rpsN"
                     /locus_tag="NK788_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCH6769332.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5104..5466)
                     /locus_tag="NK788_000989"
     CDS             complement(5104..5466)
                     /locus_tag="NK788_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCH6769333.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5477..6598)
                     /locus_tag="NK788_000990"
     CDS             complement(5477..6598)
                     /locus_tag="NK788_000990"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCH6769334.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6614..6964)
                     /locus_tag="NK788_000991"
     CDS             complement(6614..6964)
                     /locus_tag="NK788_000991"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCH6769335.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7094..8605)
                     /locus_tag="NK788_000992"
     CDS             complement(7094..8605)
                     /locus_tag="NK788_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCH6769336.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8927..10279)
                     /locus_tag="NK788_000993"
     CDS             complement(8927..10279)
                     /locus_tag="NK788_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCH6769337.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10432..11508)
                     /locus_tag="NK788_000994"
     CDS             complement(10432..11508)
                     /locus_tag="NK788_000994"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCH6769338.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11704..13449
                     /locus_tag="NK788_000995"
     CDS             11704..13449
                     /locus_tag="NK788_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCH6769339.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13729..14166)
                     /locus_tag="NK788_000996"
     CDS             complement(13729..14166)
                     /locus_tag="NK788_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCH6769340.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14150..15523)
                     /locus_tag="NK788_000997"
     CDS             complement(14150..15523)
                     /locus_tag="NK788_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769341.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15845..16333)
                     /locus_tag="NK788_000998"
     CDS             complement(15845..16333)
                     /locus_tag="NK788_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769342.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16355..16957)
                     /locus_tag="NK788_000999"
     CDS             complement(16355..16957)
                     /locus_tag="NK788_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCH6769343.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4797 a         3628 c         3081 g         5494 t
ORIGIN      
        1 cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct
       61 ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat
      121 tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt
      181 aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat
      241 agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa
      301 gcccaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg
      361 cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt
      421 agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag
      481 gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc
      541 ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc
      601 aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat
      661 tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat
      721 catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga
      781 cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac
      841 atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg
      901 attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc
      961 aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt
     1021 gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc
     1081 gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat
     1141 tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt
     1201 tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg
     1261 gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa
     1321 gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa
     1381 tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa
     1441 tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat
     1501 gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg
     1561 tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt
     1621 tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc
     1681 ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat
     1741 ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg
     1801 atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga
     1861 taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg
     1921 ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc
     1981 gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt
     2041 tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa
     2101 cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt
     2161 gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc
     2221 acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat
     2281 cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa
     2341 atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg
     2401 caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac
     2461 gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt
     2521 cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc
     2581 gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt tattgatact
     2641 tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact
     2701 ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt
     2761 gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt
     2821 gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc
     2881 tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg
     2941 ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc
     3001 tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt
     3061 atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat
     3121 catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag
     3181 caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc
     3241 agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc
     3301 gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa
     3361 ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa
     3421 gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac
     3481 aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg
     3541 atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta
     3601 agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg
     3661 attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa
     3721 aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg
     3781 ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg
     3841 attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca
     3901 aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag
     3961 attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct
     4021 cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa
     4081 gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt
     4141 tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg
     4201 acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata
     4261 ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct
     4321 cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca
     4381 tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct
     4441 ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac
     4501 gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca
     4561 aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac
     4621 gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg
     4681 ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa
     4741 aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat
     4801 tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt
     4861 cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca
     4921 gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga
     4981 tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta
     5041 tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta
     5101 tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc
     5161 aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc
     5221 gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc
     5281 tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac
     5341 aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt
     5401 tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt
     5461 taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag
     5521 ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt
     5581 ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga
     5641 tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata
     5701 gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga
     5761 tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga
     5821 gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt
     5881 ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat
     5941 tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag
     6001 gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg
     6061 tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac
     6121 gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac
     6181 gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct
     6241 ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat
     6301 gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag
     6361 taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag
     6421 ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt
     6481 ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt
     6541 cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg
     6601 tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag
     6661 taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt
     6721 cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt
     6781 tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag
     6841 gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt
     6901 tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta
     6961 tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa attataacca
     7021 ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac
     7081 tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt
     7141 gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt
     7201 ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat
     7261 ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca
     7321 ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag
     7381 aatattcttc taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa
     7441 gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg
     7501 ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt
     7561 ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac
     7621 gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag
     7681 tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat
     7741 gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga
     7801 ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt
     7861 ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga
     7921 tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca
     7981 tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct
     8041 tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg
     8101 aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca
     8161 aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac
     8221 gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa
     8281 tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata
     8341 attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt
     8401 cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg
     8461 tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg
     8521 cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg
     8581 ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt
     8641 agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg
     8701 gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga
     8761 agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct
     8821 ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat
     8881 aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct
     8941 gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc ataatactat
     9001 ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat
     9061 agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt
     9121 ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat
     9181 gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc
     9241 cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat
     9301 tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt
     9361 gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc
     9421 gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg
     9481 taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg
     9541 catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta
     9601 aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt
     9661 cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg
     9721 cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga
     9781 tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt
     9841 gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt
     9901 tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc
     9961 ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca
    10021 tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag
    10081 gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc
    10141 catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa
    10201 attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt
    10261 cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc
    10321 ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa
    10381 gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt
    10441 tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc
    10501 aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc
    10561 cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa
    10621 gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc
    10681 ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt
    10741 attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa
    10801 agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc
    10861 ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc
    10921 ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa
    10981 catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca
    11041 ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata
    11101 cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg
    11161 catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa
    11221 aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt
    11281 aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc
    11341 aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag
    11401 ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga
    11461 cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc
    11521 ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg
    11581 aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt
    11641 gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg
    11701 atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat
    11761 tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt
    11821 gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg
    11881 ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca
    11941 ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc
    12001 gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag
    12061 ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc
    12121 cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact
    12181 agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg
    12241 gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa
    12301 gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa
    12361 gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt
    12421 atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac
    12481 tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg
    12541 ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc
    12601 catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt
    12661 gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt
    12721 aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa
    12781 aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa
    12841 gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa
    12901 gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat
    12961 atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct
    13021 gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg
    13081 gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg
    13141 cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa
    13201 gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat
    13261 cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa
    13321 gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt
    13381 gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg
    13441 aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta
    13501 cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct
    13561 gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca
    13621 aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc
    13681 ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc
    13741 cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt
    13801 tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc
    13861 gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa
    13921 tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc
    13981 gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt
    14041 agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata
    14101 ggattctcct aattttatga catcttcttt tactggagaa ccatctccat caatgaatag
    14161 cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa
    14221 gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt
    14281 catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa
    14341 acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga gtagtatact
    14401 tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta
    14461 gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat
    14521 ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag
    14581 accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg
    14641 cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa
    14701 aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta
    14761 gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga
    14821 acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg
    14881 catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag
    14941 ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg
    15001 tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg
    15061 gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact
    15121 ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag
    15181 caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa
    15241 aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca
    15301 tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca
    15361 tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta
    15421 gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg
    15481 tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa
    15541 cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta
    15601 cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt
    15661 acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc
    15721 tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg
    15781 agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct
    15841 atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat
    15901 ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat
    15961 gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt
    16021 cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata
    16081 atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt
    16141 cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt
    16201 ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa
    16261 cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt
    16321 ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga
    16381 ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa
    16441 tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt
    16501 ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc
    16561 ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg
    16621 ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac
    16681 aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc
    16741 ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg
    16801 catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat
    16861 gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct
    16921 gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc
    16981 atttattaaa actgcctcct
//
LOCUS       DAHUWM010000044        16572 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHUWM010000044 DAHUWM010000000
VERSION     DAHUWM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.044"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            135..1307
                     /locus_tag="NK788_001000"
     CDS             135..1307
                     /locus_tag="NK788_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCH6769344.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1294..1998
                     /locus_tag="NK788_001001"
     CDS             1294..1998
                     /locus_tag="NK788_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCH6769345.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2003..3163
                     /locus_tag="NK788_001002"
     CDS             2003..3163
                     /locus_tag="NK788_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCH6769346.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3239..3517
                     /locus_tag="NK788_001003"
     CDS             3239..3517
                     /locus_tag="NK788_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCH6769347.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3498..3869
                     /locus_tag="NK788_001004"
     CDS             3498..3869
                     /locus_tag="NK788_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCH6769348.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3885..4289
                     /locus_tag="NK788_001005"
     CDS             3885..4289
                     /locus_tag="NK788_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769349.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4286..4696
                     /locus_tag="NK788_001006"
     CDS             4286..4696
                     /locus_tag="NK788_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769350.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4757..5344
                     /locus_tag="NK788_001007"
     CDS             4757..5344
                     /locus_tag="NK788_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCH6769351.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5417..5884
                     /locus_tag="NK788_001008"
     CDS             5417..5884
                     /locus_tag="NK788_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCH6769352.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5930..6238
                     /locus_tag="NK788_001009"
     CDS             5930..6238
                     /locus_tag="NK788_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769353.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6433..13167
                     /locus_tag="NK788_001010"
     CDS             6433..13167
                     /locus_tag="NK788_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCH6769354.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13171..14043
                     /locus_tag="NK788_001011"
     CDS             13171..14043
                     /locus_tag="NK788_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCH6769355.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14040..15089
                     /locus_tag="NK788_001012"
     CDS             14040..15089
                     /locus_tag="NK788_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769356.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            15093..15917
                     /locus_tag="NK788_001013"
     CDS             15093..15917
                     /locus_tag="NK788_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769357.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15935..>16572
                     /locus_tag="NK788_001014"
     CDS             15935..>16572
                     /locus_tag="NK788_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCH6769358.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         5929 a         2545 c         3536 g         4562 t
ORIGIN      
        1 tgaatatatt aaagaattag gatccgtaga atataaaaaa atagctaatt ttgaaagttt
       61 atataaactc tttaatgaaa ctaagctcta gaaatagggc ttttttattt ttctttgaaa
      121 ggtggtgaaa acatgtgagc ttatttgata tctttaagca gtcaataaga aatgaagagc
      181 cttctgagtg gataccggat tttgtatatg gagaggaagt atctgctcgg gcatacctca
      241 aaattatggc aaaaaatact gttttagatt ttgttgcaag aactatgtca acgttagaag
      301 ttaagtttaa aaataaagat gggacatcag actgggatta tattctaaat gttcgaccta
      361 attctgatat gtcagcagct acattttggc aaaaattttt ttatcggctc ttagatgaca
      421 acgaagtgtt agtaatattt acagatgata atcaattgct gattgctgat gattttagtc
      481 gaacggagta tgccgtgtat gatgatgttt ttacaaatgt gatggtaaaa aattttgtct
      541 ttgaacgttc atggaacatg tctgacgtca tttatatgga atataataac gaagaattag
      601 aaaggtttac acgaggttta tttgaagatt acgccgcgtt gttcggacgg attctagaag
      661 ttgcaatgcg taacaaccaa attcgtgggt ctgtatccat tgattcaacg ggaacggcga
      721 atgatgaaaa aggaaaagat ggtaagacta gatctcaaag attacaagaa tatattgata
      781 ggatttggaa ctcttttaaa actaactcag ttgcaattgt tcccaaagtg aaaggctttg
      841 agtatgagga atacaccaat aaacaaggcg tatcgaatca atcgctagat gaactgaata
      901 agatgaagtc gtctttaatt gatgatgttg ccaacgccat aggagtacct acggcgctta
      961 tttatggtga aaaagctgaa ctggattcaa atattaaagc ttttcgtaaa ttgtgtattg
     1021 ctccactagt taaaaaactt caagacgaat tggtggcaaa aataattagt cgtcaagagt
     1081 acaaaaacgg ggaacgtata gaagttaaaa aagttctcca aaaagatcta ttagagtatg
     1141 ctactcaaat tgataaaata acatctggtg gtgcgtttta tattgacgaa gttcgtgatg
     1201 aagttgatta tgatgagttg ccaaataatg aaggaaaaat tcgtattcgt acgaaaaact
     1261 atgaagaaac tgtgaaggga ggtgagaacg acaatgccga aagctaaaaa agtaccattt
     1321 cagtttacta acgaaattca gaatggtaag cacattctca ccttgagtgg aaatgtacag
     1381 aaaaaatatt ggcgtgatga tgatgtcata aacgctaaag atattcgtga ctctctagat
     1441 actgtaaccg atgatattgt aatcaagttg aacagcccag gcggtgatgt gtttgaaggg
     1501 attgagattt acaattattt aaaagatcac ccatcaaata ttactgtaga agttacaggg
     1561 cttgccgcaa gcgcagcaac gtttattgtc gctggtgcca atgatgtgat tatgaatgtt
     1621 gggacttctt tgatgattca tgaagcttct acatttgctt gggggaataa acaggacatc
     1681 caaaaaacgt taaacgcatt ggaaacgatc gatgattcta tcttagctat ttattcagat
     1741 aagactggcc aatctgttga taagttgcgt gaatggatga atgaagaaaa atggtttaca
     1801 gcagatgaag ctgtcaaata tggatttgcc aattctgtga agcgtgaaga accacaaaat
     1861 gaacctcaaa atattgcagc gatgattcaa gatgctgttg ccgttgcaat agcgaattta
     1921 aaggaacctg ttactaataa aatcgaacaa gaatcaaaac aaaaatcatt aatcgcacga
     1981 ttgcgaaaag gagaataaag atatgttaaa aattacagat aaaactgcag atgcaaaaaa
     2041 ggcatttaat gctatttctt cgaaagaaga tgcaacaccc gaacaaatta atgctgctct
     2101 agaagaatat gtaaccgcca ttgcagaaga tgccggcaaa caggtgcgtg ctgaatatga
     2161 agaattgaaa aatgtgacgg acaaccgtgt attagaggct cgtggaattc ctacactaac
     2221 agcagaagaa acaaagtttt ataatgaagc cgtaaaatct ggaggtttcg attctgacgt
     2281 agtttggcca gaaacgattt tggaacgtgt ttttgaaaat cttcaaaacg atcatcctat
     2341 tttaggcctc attaacttta caccaactgt aggtcgtgtc aaagtaatta gagcacgtcg
     2401 taaaggtgtt gctgtttttg gaccattgca taaagacctt gaaggccaac tagatgctga
     2461 atttggtgca actgaatttg ttcaacttgc gttgaccgca tttttcttaa tttctaatga
     2521 taccttagat ttaggaccac gatggattga ccgttttatc aatctttctt taagtgaagc
     2581 agtacgtgat gtttgggcta aaaaaattat tacaggttct ggtaaagatg agccaattgg
     2641 attgcttaaa gatctagatg gtgcggtcac aagtggtgta tatccagata aagctagcgc
     2701 agggacatta acttttgcga aagagaagat tgttcttgaa cttgcagggg tgatgaaaaa
     2761 attatctaaa tacacataca aagtagatgg tgacgatgaa ggtgaagtga aataccgttc
     2821 tgttgctgga cgagtgtatc taattgttaa tcctgttgat tactgggata tcgtagctag
     2881 agttacattt gcaaatttaa acaatgtgta tgggactacg atgccattta tcgctgttga
     2941 tcatatcatc gaatctgtag acgtaccaga aagtaaatgt attgcatacg ttgataaaga
     3001 gtatgaagct actcaatcaa gagctgaaaa agtctatgta tataaagaaa cttttgcaat
     3061 gaaacgcgca actttgtatg ctgtcgacat gcttggtaat ggctatccaa caaataatga
     3121 tgcagcacaa gtatatgact tggactttac agcgggggag taatgtcccc tgcaattggt
     3181 gagataacac cgacgactga tggggcagtc attaatcttt cgtaaatggg gttagagtat
     3241 gaacactgaa gaatttataa aagaatataa ggcccgattt cgtattttcc atacttccga
     3301 agatgaggat atcgggaaac agttagagag cgggtttgcc gatataaaat ctctaatcgg
     3361 cagttttgat ccttccactt atgagaaagg aaaagaatta gtttatgaac gtactcggta
     3421 tctacggaac gaatcactag agtactttta cgacaatttt caaacaatga ttatggatgc
     3481 atcaattgat ctggcaggtg ctggatatgc caattaaatc aaattataag aagcctaaaa
     3541 ttgtagctgg tgatctaaat acgccagtta ctttttttga gtttcaacct aataagggtc
     3601 cagaacctgg tgatgaagaa aaaaaggaac tgtatttttg tacagcacta gtttacaacc
     3661 catcaatgaa agatcgtgac attcttggca caaaagaagg cgttacgata aaaatacggg
     3721 atccccatca atcttatatt cctactaaca aacataaagt aaggatatta gattatcgat
     3781 atggagataa agtttgggaa ataatcgatg tctcaccgga tttggaagaa aattcaattg
     3841 taaagatagt attagaatta attacttaaa tttggtgata aatgatgagt aacgtaaagt
     3901 ttgaaggcgt tcaagaaaca atccaagctt tagaaaaaaa gtatggagaa aagaaaacta
     3961 agacattaac aaaaaaagca atcaatgttg gcgctgaaaa agtagaaaaa cagttgcaaa
     4021 cggacatgac cgtttttaaa gacaaaggtt atacgatcgc tgaggttgtt cgcaagaacg
     4081 ctacttatag agattacaag gcagaagctg aaatcggttg gaatggtcca catcaacgat
     4141 atagaatcat tcatttgaat gaatggggat atacacgaaa gggcaaacaa attagaccga
     4201 gaggatttgg tgtaataaca aaatctctta agaattccga gctgttgtac tttgatgctg
     4261 taggagagga ggttaaaaag agtttatgaa agatatgctt aatatcattt acgaggctct
     4321 ttgtttgaat gagtatattc attcaatgac ctacaacgag aataccgagc aatatcgtat
     4381 taaattttat gagcaacctg aaaccgccga taaaacaggt ccaatgatca caattcgtcc
     4441 tgtagataca cctaacgaag cgtaccacgg aagtaacaaa gagctttctg tggagcatat
     4501 gatacagatt gacgttgaat cgaaatatag agcaacatgc aagcagattc aatatgagat
     4561 aaaaaaagag atgaaaaaac taggctttgg ccaaatttcc ggtcaagggt tagatgaata
     4621 ctttactgaa acaaaccgct ttgtagacgc gcgtcgttac gaaggaaata caaaaattta
     4681 cgataccgaa tattagaaat taagacacga aaataacgtg tcttttttta ttacccaaaa
     4741 ttaggaggaa aattttatga ctttagtagg attcaaaaaa atgacaatcg gggtctttga
     4801 cagcacaggg aagattccag cagcaaattt atatgtaatt gaaggtgaac aaaacaaagg
     4861 ggcgactgta tctgctgaaa tcagtgggct atcaaaagaa ccatcaaaag tttacggatc
     4921 gaacatcgct tactatgtct ctcaaaaagg aacaggcgat gtttcagcta cttttggatt
     4981 gttagattta ccaacagaag taaatgacaa aattcttggt tacaaaaccg atacaaacaa
     5041 aatcagcttc ttgggagaag atactgagcc accatattgt gcaattttga tggaatcgga
     5101 ggatttgaac ggcgatacag caatgcttgc gatgttcaaa ggaaaattca gtcgtgaatc
     5161 tattaatctt aacactacga caaatgaagc tttcgaacca gaagcagaag aatatgtatt
     5221 ttcagcgatt gccaatgatg ttgaaggtga tgctaaaggc caaacagtcg caaaatatgt
     5281 tggagacgaa gaagcttcta ttactggttt gaaagagatg gtatttcctg cagcggggga
     5341 gtaactagcc ccgttgttgg agcaatcact cccactatta atggggcaac agttgaatta
     5401 agttaggagg ataactatgc cagatacatt taaaatctat aaaaaaaatg gtactgaatt
     5461 tacaaaagtc gcagagggtg aaagtccact ttctattact ggaattgcag caaatactca
     5521 agttgctaaa ggagattatc aagctacgcg tgtggtcgga ggtcaagaat cagtaaaagt
     5581 agatattcct gcattcacca ctcttccaat ttcagttact ggtgtgacgt tggataaaac
     5641 gacagctgaa gttgaacaag gcggaacctt aaaattaact cctactgtaa caccagcaaa
     5701 tgcaacagac aaaacaggaa gctggagcag ttcgaataca gctgtagcta cggttactgg
     5761 aggcaacgtt acagtaaaaa cagacgctga agttggcgga actacagaag tttcatttac
     5821 tactacggat ggcggtaaag tagcaaaatg tactattaca gttattgaaa aagcagcggg
     5881 ctagtcttcg gactagcttt tttatttgga aaataatgga ggaatctaaa tggcaaaagt
     5941 aagaattgaa ttgaaagaag aatcaggaaa gaaagtctat gaaaacatag acacaacagg
     6001 gaaagattat cgaaaagctt tagaaacaat caaaaagcta aatgaagacg gagtaatgat
     6061 ttggaatcgt ttagacattt atttggattt tgctgtaggt atcttcaggg atagcaaatt
     6121 gacagctgaa caaattcttg atggcttacc atctgagaag gtaatgagca cattggacga
     6181 catcctagga gaagtaatgg ggattgaaag taatccagac ccagacgcaa aaaagtaaca
     6241 cccgaagaag cagaagaaat gtacttaaac ctatgcagag agttagtgaa agcgggatgg
     6301 tcactatctg acatagaaga taattccttt gaaatgatga tgaaaattgc gtgcacttcg
     6361 cctaagaaag agaaacaaaa agaagttgat cttaaagact tcttgaaatc catgtagaga
     6421 ggaggtaaac atatggcaaa tggaaaacct ttaggcaata tgattatcaa actagactta
     6481 gacagttcag ctttttcaaa aggtctagcc ggagctaaaa acgctgttaa ccatcaaatg
     6541 aaagcaatga aatcacagat gcaagtaatg aactcttcag gaaattctct cggtgcttta
     6601 caagcaaaat acaatggttt aggcggagtt ctacaagcaa atgaaaagca agtagaattg
     6661 ctcactaagg cttataagga tagttttgac tctaacggaa acgctactgc ttcgactgct
     6721 aaatacgcta atcaattgaa tcaagcaaca gctagatcag caagttacga atctcagatg
     6781 aaaacaactg ttggacagat tgctcggatg aaagtggaaa cggaagggtt tacggggaaa
     6841 ctaaaggcta attctgatgt attgattaat tctgggaaaa aaattgaagc ctttggtaaa
     6901 ggagtttctg ggataggatc tgccctaact gttggagtaa cagctccaat agtggcaggc
     6961 gcaactgctg ttactagtgc agctatttca tgggaaagtg cttttgcagg tgtaaaaaag
     7021 acaaatgatg aagttgtaga ctcgaatgga aacgtagttt attcctatag cgacttagag
     7081 aaaggtctgc gtaatcttgc cacccaatta cctggtagcc atcaagaaat agcaaatgta
     7141 gccgaagcag ccgggcaatt aggtataaag actgaaaacg tagttagctt cactaaaacg
     7201 atgatagaca tgggtgaatc gactaatatg tcggctgaaa ctgcggcaac ttctttagca
     7261 cgatttgcta atattaccca aatgtctcaa aaagactttg ataaattagg atcagtcatt
     7321 gttgatctag gtaacaattt tgctacaacc gaatcagaaa tcactgaaat gggattgcgg
     7381 ttagcgggag ctggtaaaca gattggaatg agccaaggcg aaattctagg atttgctact
     7441 gctctatctt ctgttggtgt cgaagctgaa gcgggtgggt ctgccttttc gaaggtcatg
     7501 atccagatgc aactagctgt agaaaaagga tttggcgctt ttgatcagct gaaacagatg
     7561 gcagaagaac aaggtgttcc gtgggttaat ttagtaaatg ctgtgagaga cggtggaaaa
     7621 tcgttaaaag cagtatcaga gcaaatggga ttcacttcat ctgatttaaa gaaaatgtat
     7681 aaagaagcgg acaattcgaa aagttcgctt gaaaattttg cgaatgtggc aggtgttaca
     7741 agtgaacaat tccagaagtt gttcaagagt gatccttcag aagcaatcat caagttcatt
     7801 caagggctga aagattcaga aaaacatggt acttcagcaa tcaaagttct ggatgacatg
     7861 gatataaaag aagttcgact acgcgacagt ttattgagag ctgcaaatgc tagtgacata
     7921 ttcaaaggag caattgaaaa aggaaacggc gcgtggaaac aaaatacagc tttaacaaat
     7981 gaagctaata aaagatacga aacaactgaa tcaaagctaa agatattgaa aaacgaagtg
     8041 attgatacag caatcgatct tggaggtcca tttgtagacg cgttgcgtga tggtctaaat
     8101 gtatcgaaac cattgataaa aaacttaggg aatatggcga aagctttctc agctatgagt
     8161 gatgatcaac aaagaaacat tatcaaatgg gttggattag cagcagcagc tggtccagcg
     8221 ctgaagcttt taggaactgg tacttcagta ataggtaaag ttacttcaac tactggaaga
     8281 ttgacgaaaa gccttgttga tttagcagcg aaagcagcag aaaagaaggc tatggatgcc
     8341 tttgcatcgt cggtaacaac agctggtgct gcagctgcaa agacagcagg agctggaggt
     8401 attggcgggt tagtccctat gatgggaaaa acagctagtg ctgcaagtac tgctgcgggc
     8461 gccagcggaa taggcgcaat gacaacgtca ctaggtttgt taggtcctgc tctacttggt
     8521 attgttggcg ttggtggcgc actggcagtt ggctatggag catggaaatt atttggtgaa
     8581 gaagcttgga attcctcgca aagagtaaag caatggggaa cagatgtcgg tgaggagata
     8641 gatagtactc ttgacggagt acagaagaat attgaaggtg caaatggtca attcggtctt
     8701 ctcaaagagg gttttactgt tgatgatgca aataatatgg cggctaattt cgcaaccgct
     8761 ggaacatctt tggaaacatc tttaacgggt agaataaagg cattagacga tgcactaaaa
     8821 gggctacctc aatcagttca ggatgccatg aaagaagtag ctgaatcaga agaagaaacc
     8881 ttagggcaat ctctttctaa aataaaagac aacaatgaag aaataaaaaa aatcagagaa
     8941 agagccgcaa atcagaacag agacttgact gtttcagaac tacaacagat acaggatcta
     9001 atgaaaggaa cttctcaagc gtatgtggat acgctcgatg tttctgctaa agacagaaaa
     9061 aagatactat cagccatgaa tggtgatgtt gcacaagcta cagaagaaga agcgaagacg
     9121 tggttacaat ctttagcgaa acaaaaagct gcaactaccg aaaattatag acaaatgctt
     9181 gaagaaaaaa aggcggcttt aaaggaaaag ggagtaagca aagaattcat tgatgcatta
     9241 gaagatgaaa tgaatgatta catcaaaact acaaatcaag gattcgatag ccaaatagct
     9301 gctataacaa ataaatatcc acaattgatt aacgaagtga gtttatcaaa tggtcaatta
     9361 tttagcaaaa cggcagctgc tatggacaat actggtcagt atacaaaagt ttttctcgaa
     9421 agtaatgaaa agattttagc ccaagcgaag aaaacatcgg atgaaatagc agataatgcc
     9481 gaaaaaaatg cagaacaact tggtatggta gctgatgagg ctacgcatgc tggatcaact
     9541 tggaatggct tagttttaga cgaaaaaaca ggaaaagtaa aaactaatgt acaagaagta
     9601 gtgaatgaag caacgaagga tttcactaaa tggaatgaat tgaaacctgt tgttcatgat
     9661 gcgaaactaa caacgaatgc aaaagatgtc atttccatag cggcagtaca aaatggctat
     9721 tgggatgata tggaatggaa agaaaaagaa gcattattag aagatgagtg ttctaaaaat
     9781 gtatacaagg ctttagaaaa tgctggtaaa tggaatgagt tagatattcc ttctaaagaa
     9841 gcaatcctaa cttcaaatac tcctgaagtt atgggtgaaa ctctcatgaa tttgggatta
     9901 tgggatactt acaaacttca agtgaaagat ttagatttga atgcgtataa agtatacgat
     9961 gctatcgatg gatcagaaga aaaattaaat agttgggctc aattagataa ccccacaaaa
    10021 gaattgttat tagataactc agatttttct acaaaacttt ttaattccga agaaatgttt
    10081 aaacgttgga atcagttatc atctaatgca aagtttcttt cggctaataa tactaaattt
    10141 acagaggttc ttttatcatc tgacagaatg tggaatcaat gggtaaacct tcctgataat
    10201 gagaaaagat tacttgcaga taacgaagat ttagctacca ctgttttatc ttcagaagaa
    10261 atatacggaa gatggactca attaccagat aatcaaaaat atatgttagc tgacaatact
    10321 gacttagcaa acaaaatatt cgcttcagaa gaatcatgga atgcatggaa agcaattcca
    10381 gacacagtta aaaacttgct tggtaataat attgatttac tggctaaagt gaaagacggg
    10441 actgtaagtg tagaagaata taacaaagtt atgccacttc ttaaaactct atatggagat
    10501 aacgttaatt taaaagaggc aatcagtggg gctaaaagct ctatagagaa ttacaacaaa
    10561 aatatttttc ctagtgataa aactgcagta ggacacgatg aggcttcaca agctgctaaa
    10621 gatgcatttg aagcgttcaa catcttccaa acaaaaatac cagataaaat tactaaaact
    10681 gtatcagctg actttatagg tcctatgccg aaaaatgcga aaggaacaaa cttccatcct
    10741 ggcggagcag ctatggtaaa cgatcaaaaa gggcctactt atgaagagct tataaccttg
    10801 ccaagcggag aaacttttat acctaaagga cgtaatgtcg ttttagactt accaagaggt
    10861 tcgaaagtac tgaacgccac taaaactaaa cgtctagtgc ctaaatatgc tgatggtata
    10921 ggaaacataa cgacggtttc atctacgcta aacttagatg ctttaatttt agctattaac
    10981 gagctaacga cggtattgag aactcaactg atggcaggaa cgactgatgc taaagatact
    11041 aaactatcag atgaaacaaa agttgccgat ccgattattc cggacagtct ttctgaaaaa
    11101 tcagatcagt atctaggaat tggagctcaa tggttaacta atctaatgaa tggttggaac
    11161 tcagcggttc ctcaatatat gaatagtgaa atggttttta ttacaaacta tctcaatgcg
    11221 ctaaaacttc aaaacaatcc taattatacg caaggagcaa catggaataa gaacttactg
    11281 aatggatgga atagtttaac tgggaccttt attgctacaa tcaattcatt ttgtaatcaa
    11341 gcaatggtca cgcttagaaa ctacaacact cctatgtaca acaatggccg aacgtggcag
    11401 caaaataatc ttaatggttg gaattcattg tatggatcat tcatagcccg tgtaaatcag
    11461 cttggtaacg attcgattaa caaccttcgt tctaaaagtg gtggttttta caatgcaggg
    11521 acattcttgt tgcaatcttt aataaacgga atgaattcgt taggtaattc tctttctact
    11581 acaatgaaca atgtcgctaa tacaatggtt ggtggtatag gcaaaggagt taatggcgtc
    11641 atatcgggag ttaactatgt gcttaaggaa gtcgaatcaa gtaagaatat cggaaactgg
    11701 ccaattccac aatacgctaa gggaactgat ggacatcctg gtggacttgc tatgattaat
    11761 gatcaaaaag gcctagttca cgaagaatat gttcagatgc cagatggacg tagttttatt
    11821 gccaaaggta gagatcttct tgttaatttg cctaaaggtg ctcaggtctt aaatgcttct
    11881 ttaacaaaga aattaaagga acgtttaaat attccacact acgaaaaagg tattggcaat
    11941 ttagatattg ttgatttgtt agatgatgaa aaaagaatgt tagaattctt aactagcaaa
    12001 gttgattttt caggcattaa tgaaccatgg cttgatatga ctaaatcagg aacgtcatta
    12061 atgtccaaag ctgcaaatac aatgcttcaa tcaaaattaa gtgaattctt cactcatggc
    12121 aattttgatg gtgcagtaaa tgccaatggt gtttatcaat atttagttga tgtggcacag
    12181 aaagtaatgg gtaagttccc cggacttaca gtaacttctg gatatcgagc aggagatgcc
    12241 tactatcacg gaaaacggca agctatcgac ttggcttatc caggtatttc tggagatccg
    12301 agatatacag cagcagcaaa ttacgctttt gaaaaattcc cttcaaaaat tgcgtatgtc
    12361 attacgaatg gacgtgtacg tgaccgcatg ggattatctg gaacaaggtc aagcggacaa
    12421 tggacgaatt ggccagatgg cgatcacttc gatcatattc atttaaacgg ttcgatgggg
    12481 tctggcgata tattcaatgg aggagttgga ggaagtggtg ttgcacgctg gcgttcttat
    12541 gtgtctaaag cattgaaaat gaatggatta ccagctacag cagcgtatat aaatgcctgg
    12601 atgtcccaaa tccaaacgga atctggtggt aatgagaaag caatcggcgg caatgatggg
    12661 ctcgccgaag gcaatgcaac aggattactt caaacgaaac ccggaacttt tgcagctaac
    12721 gcttttcctg gtcacggaaa tattatgaac ggatttgata acatgttagc agcaatcaat
    12781 tatgctaaga aacgttacgg agttgcaggt atgttgcaag ttatcgggaa aggacatgga
    12841 tatgccaatg gcggcttgat taccaaagac ggactatatc gagcaggaga aggaaataaa
    12901 ccagaaatgg ttattccatt gacaagaaaa acaagagcta tcgaacttat gggtcaagca
    12961 ttagccttcc tttctggaag caataaaaaa gaaacgaaaa gtgagattaa ttctgtagat
    13021 aatagacata tcctacaagc gatagaaaaa cagtctcgta tgacaaatga tctgctatca
    13081 ttattactct cgttttttaa aagtaacaat cgatcagata aagaactagc tttagatatt
    13141 cagaaaattt taatcaggag gacatagtat ttgaggacag taatattaag aaataaattg
    13201 aatgaagaaa ttgatttgtc cacagaagat tattttgcta ctgaaatgtc gaatctaggt
    13261 tttgaagtta caaaagagca tattggacaa tgggggagtt tcagagaaat aggagagagt
    13321 attgaattgt ctgaatttca atcatcagtt atgatttctg ttcacggatt tagagaacaa
    13381 caattatatg gttcattagt tgaatttctg gctaatggac cttttgaact agaattttct
    13441 tttgattcag aaacaatgat caggaaatgt agtttgaaat cattatctaa aacagaaatt
    13501 gattcccaaa gctcattgct aacagatact ttgaatctat attttacttc aagctggtat
    13561 tctattaagc gtgaaaaatt aatacagaat tcaacttctc tcaatacacg tggaaaagtt
    13621 tactcatttg ttcgaccata tgtctacacc aaaaatgtat gggaaaagaa aggtgtattt
    13681 aagttcagta ataattctgt atatttgaca aatagtacag agcgaatgtc tccgttaaaa
    13741 ataaggatta ttggagagtg ctcaaatcca tattgggaag ttatccaaaa ctctaaagta
    13801 gtagcttcag atggctattt tattaatatg aaagacacac aaatattaga agtgtctagt
    13861 ttgctagaag accaaacggc aattttaaaa gatgtaggtg gagttgaatc ttctgtttat
    13921 caacaacaag attttactaa atcaaacttc gtccaagcgc ctctaggaga attcagcgtt
    13981 gtttttcatg ttggacatgc aaatgtagag attgaacttt atgaggagcg tgatctattt
    14041 tgattttagc cattaccctc tttcaccgag atatgaattt atataacgaa cgtttaattt
    14101 cgactgagtt cgaatttgga acggatgaaa ttggagaaga agtaagtaat tttattacag
    14161 ataaatatgt ccatgtaaaa tcaggagatt ttctactagc aaaatttatt cctagcggga
    14221 agattgctta ttttggagtg attagctcgc aagaagacaa aaaaataaat tgcaaaggat
    14281 tactcagttt ggccgatagc gagataccga ctgtaaaaat atcaggtgct aattatgaag
    14341 agcatattcg acgactgatt gaatactact tattgaacga tccaacaaaa caactaaaaa
    14401 atattttgga ggtaaaaaca gaaagcgtta cttctcattc ttatcaagct gttgacacaa
    14461 ataagagacg attgaatgca tatataataa atgggttcaa aaaatataat gttaaatggt
    14521 atgtcaaaga gattaaaggt aaaaaaatat atactggtat tcgagcagtt gatgattcaa
    14581 tttatattaa agataattca tctgagttta atgactggga tgtttttgtg caatctcctg
    14641 gaccagggaa cgagaacaaa ttattgatcg tagataaagc aatgaaagat atagaaaatc
    14701 ccttggtatt gtctacgtgg tatttagatg acgaaaataa tcttacgcaa gattcaacga
    14761 ataagaacat tgcattgcct accgttaatt tagtaaatat atatgatcaa actcaagaag
    14821 atagaccttc ttatgaagag gtagctaaat cggagcttaa aggcaacgcc tattcacatg
    14881 aaattaaagt taatgttgtg ctaaatgcta gaaatatcaa tgtagaaaat atcgaaacag
    14941 gaatgttagc gactattgtt tacaaagagc agacttacaa atctgttcta actgcctggc
    15001 gaatatccag cagtaaaaag aatatagaat tgacatttgg taatgtccga agtcgtttta
    15061 tggattattt tgaagataat gggggataaa aaatggttag caatgtggat ggatatcaat
    15121 ttgaaaacgt gaaagtaagc gcagaaaatg atgctagact ttatcacgtt ttatataatc
    15181 ggaaaaatca ggttattgat ggttacgatc agtctatgaa tttatcttca agcggattaa
    15241 cagtaaaagt tgctgcagga gcagcgatta ttcaaggtcg tatggtcgtt gttcgacaag
    15301 aagaaagtat aacagttcca gcaaactcaa gtggttatat agcattaaca gtggatttga
    15361 cacaagaagt tatacctgga tctattcttc cagaatcgga agaatatgaa tggactaata
    15421 atcaagtcaa gctagaattt ataacaaaag ttataaaagg taatttaaac aatggtgata
    15481 aggtctataa tttaccacta tgctcagtta attctactgg atcaactgtt tcaatctcaa
    15541 agatatcgga tagttacgag ctgactctct ctaaaggaga aattttgtgg agggggactg
    15601 cgttaatgca tgatactcaa actgtccaac cttcaaaaaa aatttggcag acagtttcag
    15661 gttttttgtt agtatggctc ccatacgaaa acgggcaagc aattgaggat agatatgtaa
    15721 ctacgccttt ttataaggag cgtataactt ttactaatgt tttaggagaa attgtttcag
    15781 gatttgatga ctatcacaaa aagtggttta gtaaacgaat aaactataat tcgaatacta
    15841 atatatttac aggtgcagca agcaatgcaa gtggagataa cgcaaatatg gtgcttaggt
    15901 atatagtttc tttttagtta ggaggtatgg aagagtggca aatttagaaa ttaaattatc
    15961 tgcaaataag agacagcctt atctacgtca ccgtgttgtt ggtagagttg gtgatggggg
    16021 gctcacaaca atcaatgtac aacttcttga ggaagatgaa attacacctt ttgtaattaa
    16081 tctaaacggt actttgaaat ttgtgggcga agtttcaaac ggtaactata ccgagggaga
    16141 accagaaata atcgattcga ctaatgggtt aattagttac acgttcacta agtcaaattt
    16201 cagcacgagt catcaattca aacaagcata ttttgaatat gtagatccta acggcaaaaa
    16261 agtaactttt cagaacttca tcatagacgt gttagaacga gtagatatta attcggagca
    16321 agcgaaatac tatatttctt cattggaaaa attacagagt gaaatgcaaa ccactttcaa
    16381 tcagttcatt agtgataaac aggtccaata cgatcaaatc tactcgaaat ataacgaatt
    16441 agtaagattg ataaatgaat cagataagca agtaaatgat cgtattgata aggcaaataa
    16501 tcgtattgat caaaccaatc agcaaatcgg cgatctcggc aagctgaaaa agatgtacag
    16561 taacagcatt ga
//
LOCUS       DAHUWM010000045        16470 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHUWM010000045 DAHUWM010000000
VERSION     DAHUWM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.045"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..546
                     /locus_tag="NK788_001015"
     CDS             <1..546
                     /locus_tag="NK788_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCH6769359.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="NK788_001016"
     CDS             735..1403
                     /locus_tag="NK788_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCH6769360.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="NK788_001017"
     CDS             complement(1603..1989)
                     /locus_tag="NK788_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769361.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="NK788_001018"
     CDS             complement(1961..3343)
                     /locus_tag="NK788_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCH6769362.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="NK788_001019"
     CDS             complement(3499..4686)
                     /locus_tag="NK788_001019"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCH6769363.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="NK788_001020"
     CDS             complement(4693..5724)
                     /locus_tag="NK788_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCH6769364.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5740..7194)
                     /locus_tag="NK788_001021"
     CDS             complement(5740..7194)
                     /locus_tag="NK788_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6769365.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7369..8274
                     /locus_tag="NK788_001022"
     CDS             7369..8274
                     /locus_tag="NK788_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCH6769366.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8365..9042
                     /locus_tag="NK788_001023"
     CDS             8365..9042
                     /locus_tag="NK788_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCH6769367.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9039..9209
                     /locus_tag="NK788_001024"
     CDS             9039..9209
                     /locus_tag="NK788_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769368.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9323..10219
                     /locus_tag="NK788_001025"
     CDS             9323..10219
                     /locus_tag="NK788_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6769369.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="NK788_001026"
     CDS             complement(10274..11644)
                     /locus_tag="NK788_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6769370.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="NK788_001027"
     CDS             11882..12628
                     /locus_tag="NK788_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCH6769371.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="NK788_001028"
     CDS             12625..13464
                     /locus_tag="NK788_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCH6769372.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="NK788_001029"
     CDS             13465..14415
                     /locus_tag="NK788_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCH6769373.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="NK788_001030"
     CDS             14556..15308
                     /locus_tag="NK788_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769374.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..>16470
                     /gene="queG"
                     /locus_tag="NK788_001031"
     CDS             15327..>16470
                     /gene="queG"
                     /locus_tag="NK788_001031"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCH6769375.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRK"
BASE COUNT         5349 a         3089 c         3315 g         4717 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
     1141 tagagaatgc tggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaaca actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccagct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatacctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
     5761 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca attcctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagcgatg ctgatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa gtatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt
//
LOCUS       DAHUWM010000046        16456 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHUWM010000046 DAHUWM010000000
VERSION     DAHUWM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.046"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="NK788_001032"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="NK788_001032"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCH6769376.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="NK788_001033"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="NK788_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCH6769377.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="NK788_001034"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="NK788_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCH6769378.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="NK788_001035"
     CDS             2856..3731
                     /locus_tag="NK788_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCH6769379.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="NK788_001036"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="NK788_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCH6769380.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="NK788_001037"
     CDS             4628..5062
                     /locus_tag="NK788_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCH6769381.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="NK788_001038"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="NK788_001038"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCH6769382.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="NK788_001039"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="NK788_001039"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCH6769383.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="NK788_001040"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="NK788_001040"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCH6769384.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="NK788_001041"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="NK788_001041"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCH6769385.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="NK788_001042"
     CDS             13333..14259
                     /locus_tag="NK788_001042"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCH6769386.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="NK788_001043"
     CDS             14441..15334
                     /locus_tag="NK788_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCH6769387.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="NK788_001044"
     CDS             15468..15593
                     /locus_tag="NK788_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769388.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="NK788_001045"
     CDS             complement(15727..16359)
                     /locus_tag="NK788_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769389.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5854 a         2795 c         3502 g         4305 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgt
//
LOCUS       DAHUWM010000047        16412 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHUWM010000047 DAHUWM010000000
VERSION     DAHUWM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.047"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(199..597)
                     /locus_tag="NK788_001046"
     CDS             complement(199..597)
                     /locus_tag="NK788_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCH6769390.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(594..1367)
                     /locus_tag="NK788_001047"
     CDS             complement(594..1367)
                     /locus_tag="NK788_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCH6769391.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1364..2047)
                     /locus_tag="NK788_001048"
     CDS             complement(1364..2047)
                     /locus_tag="NK788_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCH6769392.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2214..3101)
                     /locus_tag="NK788_001049"
     CDS             complement(2214..3101)
                     /locus_tag="NK788_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769393.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3098..3967)
                     /locus_tag="NK788_001050"
     CDS             complement(3098..3967)
                     /locus_tag="NK788_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769394.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4104..5390)
                     /locus_tag="NK788_001051"
     CDS             complement(4104..5390)
                     /locus_tag="NK788_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6769395.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5663..6241
                     /locus_tag="NK788_001052"
     CDS             5663..6241
                     /locus_tag="NK788_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCH6769396.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6241..7170
                     /locus_tag="NK788_001053"
     CDS             6241..7170
                     /locus_tag="NK788_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6769397.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7167..8903
                     /locus_tag="NK788_001054"
     CDS             7167..8903
                     /locus_tag="NK788_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCH6769398.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8966..9802)
                     /gene="pheA"
                     /locus_tag="NK788_001055"
     CDS             complement(8966..9802)
                     /gene="pheA"
                     /locus_tag="NK788_001055"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCH6769399.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9799..10308)
                     /locus_tag="NK788_001056"
     CDS             complement(9799..10308)
                     /locus_tag="NK788_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCH6769400.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10301..11596)
                     /gene="aroA"
                     /locus_tag="NK788_001057"
     CDS             complement(10301..11596)
                     /gene="aroA"
                     /locus_tag="NK788_001057"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCH6769401.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11609..12700)
                     /locus_tag="NK788_001058"
     CDS             complement(11609..12700)
                     /locus_tag="NK788_001058"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCH6769402.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12717..13883)
                     /gene="aroC"
                     /locus_tag="NK788_001059"
     CDS             complement(12717..13883)
                     /gene="aroC"
                     /locus_tag="NK788_001059"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCH6769403.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13885..14949)
                     /gene="aroB"
                     /locus_tag="NK788_001060"
     CDS             complement(13885..14949)
                     /gene="aroB"
                     /locus_tag="NK788_001060"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCH6769404.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14982..16004)
                     /gene="aroF"
                     /locus_tag="NK788_001061"
     CDS             complement(14982..16004)
                     /gene="aroF"
                     /locus_tag="NK788_001061"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCH6769405.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4737 a         3628 c         2908 g         5139 t
ORIGIN      
        1 actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca tattttgaaa
       61 agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata agtattcacg
      121 agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt tgtgtttatt
      181 tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt catctaaaag
      241 ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa tgactttgtc
      301 tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc cttgaccaat
      361 cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa tatctttttc
      421 tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac ctcttccatg
      481 taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat cttcgcccat
      541 tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat ctttcatttg
      601 ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc ttttcgttta
      661 taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat tgcaaccaca
      721 ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc cacgctcatt
      781 gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc aaaagccatt
      841 gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta tcctgggatc
      901 aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc tactgctgcg
      961 ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc ctcgctcaat
     1021 tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac taagaacccc
     1081 attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat agcactgcct
     1141 aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag gatcaaccca
     1201 atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg catacgaaaa
     1261 ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa aatgacttcc
     1321 atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg aagatcaccc
     1381 ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt ccaaaactgt
     1441 cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg gccaagataa
     1501 atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc ggcataaatt
     1561 cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc atcaattgat
     1621 aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact ccagtgccga
     1681 cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata tcatatactt
     1741 gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg atagcaaatc
     1801 ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct actagatctc
     1861 cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg atcatcatga
     1921 ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca aaaacaaacg
     1981 ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct ttaaaatttt
     2041 ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag agttttcgag
     2101 cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca cgtcccaaaa
     2161 gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt tttttatttt
     2221 acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac gatggcagtt
     2281 ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt ataggaagca
     2341 cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa agctactaag
     2401 ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc ggcataggta
     2461 tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag accgtctaat
     2521 cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa gaagaagatc
     2581 aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat tcccaaaacc
     2641 gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc aaaaggaacc
     2701 atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg ataaatctca
     2761 aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac caaagcgatc
     2821 atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa attttgaaaa
     2881 ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac gggtgtattc
     2941 gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag gatgcttaat
     3001 gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg ttttttgacg
     3061 ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct cctcctactt
     3121 tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa actgctgccg
     3181 catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga gatagggtgg
     3241 tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa agctgcaatg
     3301 tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt gggatcgtca
     3361 ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca taaatctccg
     3421 aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc caacgccaag
     3481 tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag ccaatcggtg
     3541 tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga gaaaatagac
     3601 ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag atagcggttc
     3661 gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata aaaagtgcta
     3721 gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa aaagcttttt
     3781 taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat gtcatattga
     3841 cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata aaaaccatga
     3901 ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat aaatctttct
     3961 gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga caagaaagac
     4021 aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg aaaaagcgaa
     4081 gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg tttttgtgta
     4141 tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc ttcagctacg
     4201 actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc agatgtccat
     4261 gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc ttgataagct
     4321 tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc aagagtgccc
     4381 atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga atcttctcca
     4441 gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg cgctttttgc
     4501 agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg gttttctgct
     4561 ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc tcctgattgt
     4621 atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc tttcaaaaga
     4681 gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt gactgtctcc
     4741 ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac tcgaccaagg
     4801 tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc tcgcgctact
     4861 tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc tgcttctgcc
     4921 atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac accgtatatt
     4981 ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc ctttacaata
     5041 gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat cctataatct
     5101 tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc ggtgttcagt
     5161 ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc ttcgttctca
     5221 taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca cgcccagaca
     5281 gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt taagaaacct
     5341 gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat ttttatccgc
     5401 tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa atttgaaaac
     5461 ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa aaatacaaat
     5521 caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg gtgaggtttc
     5581 tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa attacaagac
     5641 tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg atgaacagac
     5701 gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc aattaatagg
     5761 gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa tcgatatcgt
     5821 ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc tattaaaaca
     5881 gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg atatggttaa
     5941 agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga gtcctgattt
     6001 ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca acaaaagccc
     6061 aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg ctggacaaac
     6121 tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt atataccgaa
     6181 aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac ccattcattg
     6241 atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga agaatccggc
     6301 tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa gtttcgtcaa
     6361 gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca agatatcaat
     6421 gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca taagggaaag
     6481 aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca gcaagcaagt
     6541 agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga acttgagaat
     6601 ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca tgaggcaatc
     6661 aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa tgaagaactt
     6721 tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt cgaagatttt
     6781 tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa tgcaaaaaaa
     6841 gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta tgagaaagaa
     6901 atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct ctcagccatc
     6961 tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg tatcagtaaa
     7021 gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga gaaagtcggc
     7081 tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat gacaccttct
     7141 caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat gaactctttg
     7201 ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc atttctttgt
     7261 ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg aatgatgtgg
     7321 tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag aatacattag
     7381 ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca gcccaacata
     7441 tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt tcaaaaatcc
     7501 atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact tttgtcggaa
     7561 acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt gctcagagag
     7621 tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg actcttcgcg
     7681 ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg aatcacgttt
     7741 acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt caatctgtag
     7801 taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc atcgcatcaa
     7861 atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca gtaggtgaaa
     7921 caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag gattgtacat
     7981 tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt tttccgatta
     8041 ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg tatttggatc
     8101 gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag gaagatggct
     8161 taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg aatacgatca
     8221 atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac gaaaagaaga
     8281 aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt atttataata
     8341 cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca gttcaaggaa
     8401 tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa gtgattccag
     8461 tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg cgatatggcg
     8521 aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct atgccgaata
     8581 tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat aaaacgcaag
     8641 gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa gtgattgata
     8701 acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt tcaggaatcg
     8761 gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat ggcttaacga
     8821 tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt cttcctaaaa
     8881 atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat tttaagttaa
     8941 ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc agcactggat
     9001 aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc gcattctcta
     9061 ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct cccaagctag
     9121 ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca aaagctgcca
     9181 gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca ctcattttta
     9241 ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa cgtgtctggt
     9301 tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc tgtgctgttt
     9361 ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac attgctgcag
     9421 cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct aaaaactgtt
     9481 gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt gctggattgc
     9541 ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact tctggatgtt
     9601 tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct acaggaacga
     9661 cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg gaagcatacg
     9721 cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat gaactttctg
     9781 gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat cgatgatctt
     9841 ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat accaagaaag
     9901 tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta aaggtcgaat
     9961 agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct ctaagtaaag
    10021 aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa tccctccacc
    10081 agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact ccacttttcg
    10141 aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga tatgttcctc
    10201 aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc ctttaccgat
    10261 tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga gcttctcctc
    10321 ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa acagcttcag
    10381 ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc atcataccga
    10441 ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc ccatggatga
    10501 tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct tctgccaccg
    10561 catcaattct gttggtttct tttactttta attcttctgc gtctttgatg atcgtgattc
    10621 cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga cgcggaatga
    10681 tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa tcagccgatt
    10741 ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc aacacatcta
    10801 agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg ctttccggaa
    10861 caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga acagttatct
    10921 gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc actgtgatcc
    10981 ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct ttttcaacga
    11041 ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg acttgcgcac
    11101 tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc acagtgattg
    11161 gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt aaaggttcca
    11221 tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta gtcgtaaaag
    11281 gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt cccatgtcaa
    11341 ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg atcacctctt
    11401 cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc agacaatctt
    11461 ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca aacatgatgc
    11521 tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat ccatgtattt
    11581 gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa ctggtattag
    11641 acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg atttgcagta
    11701 ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa actcccgctt
    11761 ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca tcgataaata
    11821 aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct cgactttctt
    11881 ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc cattctgcta
    11941 tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc ttattgctca
    12001 acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata tctcgaaagc
    12061 ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct gcctgattga
    12121 ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata cggtcatgtt
    12181 ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct tgcagttttt
    12241 caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt tcaaatagtt
    12301 tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt ccaccgacaa
    12361 accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg ctgacatccg
    12421 taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa gctaaggttg
    12481 tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt tcaaaatcac
    12541 ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt gactgactat
    12601 cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc atagaagcac
    12661 caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc ccctttttag
    12721 taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac aagcgaatca
    12781 cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt ttccgcaacg
    12841 acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt gtaaggttct
    12901 tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg tttcatgacc
    12961 ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc tagattattg
    13021 gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac ttctgagcct
    13081 ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc attgatacta
    13141 accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta gcttcctaat
    13201 ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc tccatttttc
    13261 tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac aactcgtact
    13321 tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg tatttctgct
    13381 tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc ttccagaatt
    13441 ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga ggaccgttca
    13501 agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc cgcgtgtcct
    13561 ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc tgctacagac
    13621 atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac aggtgaacca
    13681 attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc gatcttcatc
    13741 cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat ttcttcttct
    13801 gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg tccatgggat
    13861 tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga atgtctctgt
    13921 gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat tcaattggct
    13981 tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat gacttagatc
    14041 gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg tcgatccagt
    14101 tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa tagctactcc
    14161 ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc caattgtatg
    14221 accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca ctacttttcg
    14281 tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat ctttttcgtc
    14341 tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg atttgatgat
    14401 ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct caggatcaat
    14461 caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt ttgtattgac
    14521 tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg gaatttgaac
    14581 gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac ccaccattcc
    14641 tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta aaaaatcgta
    14701 taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga ttgaaaggac
    14761 tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata gtggagcaac
    14821 gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa gccatgatcc
    14881 agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat caacatgtac
    14941 agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag tgtctgaatc
    15001 ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta agtcttttca
    15061 ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat ctctacgatc
    15121 atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag ttcccaaatt
    15181 cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt catcaatggg
    15241 acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat cccacgttcg
    15301 ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt aagccattca
    15361 ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt accgacagca
    15421 gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc gctgtactca
    15481 gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat tccaaattca
    15541 tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa agcatatgga
    15601 gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc tttggccatt
    15661 cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc catcgtcacg
    15721 aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc aggatggaat
    15781 tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac tccttcatag
    15841 cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat cactactcgt
    15901 tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc gacgactttt
    15961 tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt tcccgctttc
    16021 ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg ttttacatta
    16081 attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt cctacttatg
    16141 acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca gtgaacatct
    16201 tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat gaatttctct
    16261 acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac ctggtttgtt
    16321 tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat acgtattcag
    16381 ttttgtaagt tttctagatt tcataaaaaa tc
//
LOCUS       DAHUWM010000048        16271 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHUWM010000048 DAHUWM010000000
VERSION     DAHUWM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.048"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            115..1542
                     /locus_tag="NK788_001062"
     CDS             115..1542
                     /locus_tag="NK788_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769406.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1634..1999)
                     /locus_tag="NK788_001063"
     CDS             complement(1634..1999)
                     /locus_tag="NK788_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCH6769407.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1996..2361)
                     /locus_tag="NK788_001064"
     CDS             complement(1996..2361)
                     /locus_tag="NK788_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCH6769408.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2553..2801)
                     /locus_tag="NK788_001065"
     CDS             complement(2553..2801)
                     /locus_tag="NK788_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769409.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2800..2928
                     /locus_tag="NK788_001066"
     CDS             2800..2928
                     /locus_tag="NK788_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCH6769410.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2985..4679
                     /locus_tag="NK788_001067"
     CDS             2985..4679
                     /locus_tag="NK788_001067"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCH6769411.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4683..4802
                     /locus_tag="NK788_001068"
                     /pseudo
     CDS             4683..4802
                     /locus_tag="NK788_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4779..4952
                     /locus_tag="NK788_001069"
     CDS             4779..4952
                     /locus_tag="NK788_001069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769412.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5330..6463
                     /locus_tag="NK788_001070"
     CDS             5330..6463
                     /locus_tag="NK788_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCH6769413.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6494..6814
                     /locus_tag="NK788_001071"
     CDS             6494..6814
                     /locus_tag="NK788_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCH6769414.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6843..7307)
                     /locus_tag="NK788_001072"
     CDS             complement(6843..7307)
                     /locus_tag="NK788_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769415.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7439..8692)
                     /locus_tag="NK788_001073"
     CDS             complement(7439..8692)
                     /locus_tag="NK788_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6769416.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8721..9038)
                     /locus_tag="NK788_001074"
     CDS             complement(8721..9038)
                     /locus_tag="NK788_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCH6769417.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9064..9384)
                     /locus_tag="NK788_001075"
     CDS             complement(9064..9384)
                     /locus_tag="NK788_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6769418.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9848..11263)
                     /locus_tag="NK788_001076"
     CDS             complement(9848..11263)
                     /locus_tag="NK788_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6769419.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11280..12596)
                     /locus_tag="NK788_001077"
     CDS             complement(11280..12596)
                     /locus_tag="NK788_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6769420.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12746..13444)
                     /locus_tag="NK788_001078"
     CDS             complement(12746..13444)
                     /locus_tag="NK788_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCH6769421.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13733..14689)
                     /locus_tag="NK788_001079"
     CDS             complement(13733..14689)
                     /locus_tag="NK788_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6769422.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14802..15635)
                     /gene="msrA"
                     /locus_tag="NK788_001080"
     CDS             complement(14802..15635)
                     /gene="msrA"
                     /locus_tag="NK788_001080"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCH6769423.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15810..>16271)
                     /locus_tag="NK788_001081"
     CDS             complement(15810..>16271)
                     /locus_tag="NK788_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769424.1"
                     /translation="NQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEH
                     IIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQ
                     VNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5061 a         3280 c         2764 g         5166 t
ORIGIN      
        1 ccttttttaa cggcatttat atgctttttc agaaattaac tgatgatttc aaaaagaaaa
       61 catgtcataa tatttcccat attgtgcaaa aaaatacaag gcggaggctc tactatgatt
      121 gaagaatata ttgagaaaaa tattttaaga cagctgtttt tatgtgggca attctatgta
      181 aataaagaag tcaatctaga aaagctctcc aatctcctgc atgtttgcaa aaccacttta
      241 ctcaatgata tcaataacat aaaaaaagaa tttgaagaac agatcgctta tacacatcgg
      301 gaaaaggacc gttacacttt atatttttca gaacatatac ctcgatgcaa gatcatgcag
      361 caattgtctc aaaattcgtt gtttttaaaa acttgtctgc tttatttgga agaagatgaa
      421 cctgattatc ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta
      481 aaaaaacaag tattagctta ctttaatgac tgcgggatcg aaatcgaccg ctactcccct
      541 cgattcactg aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt
      601 tttaattctt gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc
      661 gtaacggaaa atagtggcag attttatgat aaagaaaata aagaaatctt atcgattggt
      721 tttgctatca gtttcctacg ccagcaagtg tgtgctgtga caatagattc aaaatttatc
      781 gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat cagcatggga aaacactgat
      841 ttccaaacct attataaaaa agaagaattc gcctttattt tgacactatt caatctttgt
      901 aattatggtt tccattctta tcagttgatt gcggaagact ttcaacagct acaccaagtg
      961 tttattgaca atactcccga aataaaagaa ctagttgcta cctttgaatc tcacttcaat
     1021 caagaattat tcggaaacca accgttcgaa cgtgcgctga ttcatctgat gcgttcggct
     1081 tgggataact atcaattatt catgccagaa aaattttatc ttttaaatga ggaacagaca
     1141 aatttatata aagaagtcca gacaattttt tccagctggt ccagccagtt accttatgat
     1201 ctacggttga atcctaattg catgcgggct tttgtgatcg aattgagcgg aattctccgt
     1261 ctgacaaaag aacatctgac tatctatatc gtcacaaatt cggatgttca ttatctgatt
     1321 tatcgggaag ccttggaagc tgtaactact ttcgattttc aagttgcacc aacaatttat
     1381 tcaagtatat ctgatataaa gaagtatgcg cagcagtcct caaaccgggt attatgtgaa
     1441 cgtacattat atacaccaga tgcggtccaa tatgaaaata ttattcctat ttcgatcaat
     1501 acgatcgatc gagctatcat ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc
     1561 atgtcacagc ctcttatttg ttgaagatgc ccatttctgt aacaatctct tgatacacgt
     1621 caggctcaca acctcactta ttttggacat ctaggggtcc gatctcttta tttaaaaaat
     1681 aagaaatgat ggttcggata actaccaacg ccgctagttg gaaaatatcc tgcaaggtag
     1741 gtttgataat cgattcgatg atcccagctg aaacaagaat ttccaaactt agaagtatat
     1801 agcctcccaa catcttttta atagcattgt tctcgcttac tcggtcgctt tgcacttttg
     1861 tatggacaaa taaatatttc aaaaaatcct tgattgatag acaaacaccc caaaccaata
     1921 aacagatagc aaagatatca agtcccagta caagtttttc gaaatatgga agaagaaact
     1981 gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt cttctatttc
     2041 ctgattcaag aaataagaaa tgactgttcg aatgataata ataagtgcaa gcttcaaaat
     2101 atctgtccaa gtcggcttga taactgactc gatgatatca gcaacgacta gtacttccaa
     2161 gcttaacaaa atataacttg ctagcagttt tcttgtatct gcattacgag tattacgttc
     2221 tgcatagttt ttcttgacat gagaccaatc aaaaagtttg aatagaacaa gaacgatccc
     2281 gacaattaag ataccgatag aacaaatatt taaaatagca atgatccatt ctagaaattc
     2341 aattaaattt gagcgaaaca tatacgctac ctactttctt ttaatttgtt cctattcttt
     2401 tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt tgttctaatg ataacggttc
     2461 gcttaaattt ttctttttca ctctcccatt tttctccctc catccataaa tcgtggaagt
     2521 atccaaaaca acgtagcaag ttaaaataag gcttagtccg tcatcagctt ttcaaagaag
     2581 cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa tagaaccata cacctcctca
     2641 aatcttttct tcttacctac cctattttta tttacaggaa aatcagacac ttcttctttt
     2701 ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc attctaataa aaacgaacta
     2761 tccttggata tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa
     2821 gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat atctaggaaa aaagacagga
     2881 ctattcgaag atgacagtca tttatatgat gaattcgaat caatctagca tcctatttag
     2941 ttgaatcact acggacatac ttggaagaca ttggaggaat cacaatgaga aaaagtaaag
     3001 caataatgat tggtgccggt attgccaata tggcagcggc tgtttatttg attcaagaag
     3061 gcaaatggaa aggggatcag atcacctttt attcattgga tgatcacggt tcaaatgatg
     3121 gtgcaccgac cgacactgta gtggatgaat attggaataa aaaccatcca ttagaaaacc
     3181 aaaaaggcta tattgctcga ggcggtcgca tgcttaatta ccgtacctat gtagatttga
     3241 tggatctgtt aagtcgaata ccttctgtta ctgagccagg gatgactgct gaagaagata
     3301 cgagagattt tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg gaaggtggca
     3361 aaggaatcat tgatggcgga aaactgggat tcaataataa agaccgtatt cttctgacta
     3421 aactgattgc tatgcctgat tcagaagaag aaaaactaga caatatcact atcgaagaat
     3481 atttcaaaga tgatccacac ttttttgaaa caaacttctg gtttatgtgg gaaacaacat
     3541 ttgctttcag aacacgtagt tctgctcaag aattgcgtcg ctatatgcat caaatgattt
     3601 atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac aatcaatacg
     3661 aaagtattat gcttcctttg atcgagtact tgaaagaaca aggatgcaaa atcattttga
     3721 accgccgtgt cactgactgg gaattcaaag aaacaccgat gcaagatgaa atcactgtga
     3781 ctggtttgaa aattaccaat acactgactc aagaagaaga atacgttgtg gtcgatgaag
     3841 atacagcagt tattttcact aatgggtcca ttacagattc agctacttta ggagattacg
     3901 aaacaccagc tgtagaaaat atggattatg gtgcagcttc tagtctctgg aaaaaagcca
     3961 gtgaacgttt ctacaacttg ggaaatcctg acaaattctt tgctgatcga gatgcaagcg
     4021 aatgggtcag ctttaccctg acgacgaaaa accatttgtt gttgaatgaa atcacccgta
     4081 tcacgactca agaaccagga aatgccttga attcatttat ttcaaccaca ccgatcacgc
     4141 cgttaggcca aaaagatgtc aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt
     4201 ctcagaaacc aaatgaaaca gttatttggg gttatttcct ttatccaaga cgacgtggcg
     4261 aatttgtgga taaacagtat attaaaatga atggaaaaga aatgctagaa gaactgattg
     4321 gacaactttc aaaagtagat cctggaccag tgaatattcg tgaaaaagaa acagaaattt
     4381 tcgacagcgt aataaataat atccctgtct atatgcctta tgcttccgcg ttgttcaata
     4441 atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta
     4501 caggagaatt tgtcgagcag ccttatcaga tgatctttac tgaacaaagt gctgtacgtt
     4561 ctggagaaat cgctgctttc cattttgcgg gtattcctat gtcccgtctt gtcaaaacac
     4621 cgcgttacga caaagatatt cctactctag ctcgtgccgc aaaaaaaatg tttgaataat
     4681 caagaggttg tgaaaaagct gccctgcatc aagtaataag aacaaccagg ctaaaacagc
     4741 ttcttatgtt tttgatttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt
     4801 taccaagagg ttatgaaaaa gctgccctgc atcaagtaat aagagcagcc aagctaaaac
     4861 agcttcttat gtttttgact tggttagact tattaccgca gatgcaagct tttgaacacc
     4921 gtttaccaag aggttatgaa aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa
     4981 aatggttcct cacgtttttg cataaatttg acttaatacc gagaaactga tcccttctgt
     5041 cataacctct ttttatttat gtctgtttct ttttgaaaac aactattttt tcaacgttta
     5101 ctctcttgcc aatccaacat catctaacgg atacaactat ttcaatgatt accattacac
     5161 taaaaaatcg gaaacacacc tctccgaaca caaatacata agactataga atgtttcatt
     5221 taagaaaaaa agcgtttcat tatcaaaaag gtaagatctc ctttaaaatg aaattatctg
     5281 tagtactaat aatgcagaca attttatgaa tctgaaagga gcatctccaa tgaaagcagt
     5341 cacttggcaa ggaaaacaga aaatggaaat cagaaacgta gatgatccaa aaatattaga
     5401 accaacagat gcaatcatcc gtatcacagc aacagccatt tgcggctccg atctgcatct
     5461 ttaccatcat ggtgaagcag ttttggaaaa agggtatgtt gtaggacacg aaccaatggg
     5521 aatcgttgaa gaagtgggat ctcaagttca aacattgaaa aaagggcaac gtgtggttat
     5581 cccttttaat attagttgtg gtcattgtca tttctgcctg aatcacatgg aaagccaatg
     5641 tgatgaatca aatccagatc agttatatgg cgggcttttc ggatttggca agttgaacgg
     5701 taatcattgg ggtggtcaag cggaatatct aagagttcct tatgcagact ctacttcatt
     5761 tgttgtacca gataatgatc ttcctgatga aaaagtttta tttctatcag atattttgcc
     5821 cacagcttat tggagcgttg aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct
     5881 aggttctggt ccagtcggtc tattcgctca aaaattcgct gttatggctg gagcagagcg
     5941 cgtaatcgca gtagatccag tacagcaccg tctagataaa gcggctaatt acaatggggt
     6001 tgaaacttat ttcttagaag acacggcaca agctggaaca gaattgtatg aactggctaa
     6061 aggtggcgca gatgtcatta tcgattgtgt aggaatggac ggattagagc ctgtcaaaga
     6121 aaaagcgaaa aatttagtaa gcttacaaag cggcacaatc tctccattac agatggcatc
     6181 gcaagctgtc aaaaaatttg gaacagtcca agtaactggt gtttatatga ctccagcttc
     6241 ttcttacccg ctgcaagaat tttttatgcg ggatatcgct gtcaagcatg gacaggcacc
     6301 aattatccat ctaatgccaa aaatttacgc aatgattgca gaaggactat tcgatcctac
     6361 acaaatcatt actcattcgc tgccactatc agaagcagca caagcatatg agatctttga
     6421 caaaaaagaa gataaaaata taaaagttgt tttgaaacct tgaattcagt aaaaaataca
     6481 ggtgattttc tttatgccca aaatcaaacg aaatacccat gagatcgaaa taggaaaaga
     6541 agaagatatc gaaatacgag gacgtcgctt ccgcttctat ttccaaaatc gttacacatt
     6601 gatctcactg gctgtcgatt tattaaccgg gattttctat atttttggaa gccttgcttc
     6661 cttaactaaa atccccgatc gatatggcac ctatttttat ttggctggtg ctattttctt
     6721 gacgattcgt cctattctca gaatcgtaag gaacattttt atttacgacg aaaaaaaagc
     6781 aatgaagaga acaagcaaaa aaaaagaggc atgagaattt ttcaaatctc atgcctcttt
     6841 tttcacccta tcgtatcact ttgatacaga cgagcggtcg tcccactttc ttccttgacc
     6901 attgtcaaaa atgtccactc gtatcgttca taaaacgtgg tatgatctgt aattaagtaa
     6961 agtttttcgt acccaagttg atgcatttct tttctgattt ccgctaatat ccgcttggct
     7021 atcccctgct tacgcttttc cggttcaaca aacagggcac aaagatttgg tgtcagatca
     7081 gggcggttat ggaagtcgtt ttcaatgacg cccgctcctc cgacgatggt ttctttttca
     7141 tctaagacca cataccattg aggaatactt tccttattag cgattccagt acgaatactt
     7201 tcctcataca caacagcagg aactccccat tttgatgaaa accaattcgc gctttgtttt
     7261 actaattctg ggtgttctct taatttgatc cagttatatg taaccaatgt ttaaacctcc
     7321 gactaaaata aaacgtttac aaaaattata caagtcttta tgaaaattag caatgacaac
     7381 aatagtgatg taaaaaaaaa aacggaaaac aatcacttgt tctccgtttt ttgattaatc
     7441 aagcatctaa agcttctttt cttctttctt ggatcgtagc taccttgacg aacggtagat
     7501 agatcaaaat agaaagaacc aaattgacag cggctagtac accacctgca atactttttg
     7561 ttgccaatac accgccaata attggtggag ttacccatgg aggcataacg gtagctgctg
     7621 gcactaaacc tgtagaagtc gcaaagtaag caacactaac taagaccatt ggtgtcaaaa
     7681 taaatggaat gaacatgatc gggttcaaca cgataggtaa cccaaacatc gttggttcat
     7741 taatattgaa gacacctggc gcaatcgaca agtttgttac aaccatatat ggtttgtttt
     7801 tacgtcctac taaataaata gctaacagta aacctaaagt tgcacctgtt cctcctaagt
     7861 tgacaaagct gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgt ccagctgcga
     7921 ttgcattaac gtttgcttca atggctggcg cattgattgt ttgcatcaaa ggatcgacca
     7981 tattcgctcc gtgtaaacca aagaaccata agaatggtgt gataaatgca agcagtaagg
     8041 cagatggata agagtttgca agtcccataa atggttcttg gaccactgta tagaaagaac
     8101 ctacgatatc atcaacaccg aatgcagcaa aaattgcagc aactaaagca aacgcagaga
     8161 tagtgatcat tgctggtagt aatgaagcaa atgcttttgc aactgccggt ggcacaccat
     8221 caggcatttt gatgatcaat ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa
     8281 taatcccaac aataatagca ataaacaaac cgtttgagct catacctaat gctccgccta
     8341 aagcgaagaa agagcctaaa gaaatcacac ctgcggaaac accgtctttt cggtagccgt
     8401 tagctaaatg ataagcaagc aagaaagcaa tcaagacaga aagaatggca aatgtaccgt
     8461 tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag ttcatcaatt
     8521 cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct aagatcatta
     8581 acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt
     8641 ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc ataaatcttc
     8701 cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagattaat gatctctttt
     8761 gctaaatctg taaaagctat agatgtcatc agatgatctt gactgtggac cgtcaaaagc
     8821 gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt
     8881 gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt gaaatcacct
     8941 tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc aaacatgatc
     9001 agacccatga tggcttctag atttttttga tcttccattt attgtatcct ccgataattt
     9061 ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat tcatcatgcc
     9121 atagtcagcc atattgatta catctaaagg aatgccttta ggtgctagct tttcagcaaa
     9181 ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat ttttagattc
     9241 tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca ttccttttgc
     9301 ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag ctgaacatac
     9361 aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta cacttgtata
     9421 taagcaagta gcgtgccaac tttaaaagcg ctttaaaaag ttgatatcat cacatttaag
     9481 gtctatacca agttgcatac acactaaaaa agaaagcgca cactaaacac acaaattaat
     9541 ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca
     9601 acttttttca aataaacggt attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt
     9661 taaaaacata agatgttgtt tttactttgc tattcttatt acttgatgca ggacagcttt
     9721 ttcacaacct ctcaataaac ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc
     9781 tggattcaca acctctttta tttcaaataa ccagtaaata caatgaacat ctacaaaaaa
     9841 cttctattta tttatagatt tcgtcaggca aatccaattc tctcttatca atcaaagtct
     9901 gataccacga agcagatttt ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa
     9961 ctaatccata tcgattctta aatgcattca ttggtgacac acagtcagtg aatgcccata
    10021 acatatagcc ttcgcaattt gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct
    10081 gtccgataaa ttcgatacgg taatcatctt gcacgattcc tttctcatcc atatactgtt
    10141 cttcatattc tcttcccata ccgttttctg ttatcagcca agggatatta tgataatttt
    10201 cttttagata catggctata tcatacatga ttttcggata gatttcccat cccctggaat
    10261 tattcatttt cttcccaggt aaatcaaaga cttcataata tcgttcagga tggaaaggtg
    10321 tctcttcatt ccattgataa cgaggggcgc gaacgcgttt aggataatat tgattgattc
    10381 caaggaaatc tactgtatga tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat
    10441 aaatcgattg tttttgacac aactccagga actcttctgt gtagtatcct tttaccatcg
    10501 gatcaaaaaa tactcgatta taaaacaaat catacatata agcagctttt tggtcttctg
    10561 gagaattgga tcgagcatag accatttcag gatttaaaat acagccaatc cttccaccat
    10621 aatttccgct gcggtaaatc tctactgctt tagccgttgc taaaacttta tgataattcc
    10681 acatcatcca cttttttgtg ttttgttcat gcggccagcg aacagcatcc aaatagcaac
    10741 gcgtttgggg aacgattggt tcgttgaatg taaaccagtt ttttacccta tcaccgtaac
    10801 gatcaaaggc aatttgagca tacttagcat aaaaatcaac tacttttctt gaagaccagc
    10861 catcatattt ttccaaaaga aaagctggga cttcataatg ttccaaacaa atcatcggtt
    10921 ctacgtctgc ttcgatcaaa gagtcgatca tttcacttat atgctgagca tattcttcat
    10981 ctactataca gttctcataa tcagtaaaaa aacgagccca attgatagaa gtccgataat
    11041 gtgtcagatg gatggatttc atcagatcca catcctgctg gtattgttcc ataaaattag
    11101 ttgctacggc tgggccatac ccattatgcc aaacaaaatg ttcattttta taccagctat
    11161 ctataaacga atcctgtcct tctttttttc ctttccaacc ttccgtttgc caagcagaag
    11221 atgcagcacc aagaataaaa ttttttggaa tagtaatttt catgatttcc tcctgttttt
    11281 tagatcccat cattttccac acgatttgct acttttacaa atggcagata gatcaaaata
    11341 gaaacaataa tacaaacaag ttgcgtaata actgcaccga tatttcctgc tgttgccaga
    11401 taagcattga cgatcggcgg cattggccaa gggaccatta caactgcttt cgccgcaaat
    11461 cctaaagcgg ttgcaaaata accaatcgtt ccagtgataa gaggcgttaa gataaacgga
    11521 atagctaaaa ttggattcag catgatcggc ataccaaaaa taactggttc attgatgttg
    11581 aagatcccag gagcaaggga taacttggtg atttgtttca tatcttcccg ttttccaact
    11641 aacaatactg caatcagtaa accgatcgtg actccagacc cgcccatact catatacata
    11701 tcccaaaatg gcatagtaat aatgttcggt acttcttttc cagcttcaaa agcttccgta
    11761 tttacagcaa ttgccgctaa aagcaacggt tcccgtatcg gcttcaccat ttgatttcca
    11821 tggataccaa tcacccaaaa gacttgcgca ataaacatca atagcaaaat acccggtaac
    11881 ccttgtacga caccttctaa tgggcgttgt acgacattgt aaatgatatc ataaaggtac
    11941 attcctgtaa gttctttgat gataaatccc gcagccgcga tacccgatac agtcagaatc
    12001 gtcgggaata aagcagaaaa actagcagaa acattcgttg gcacggtatc aggcattctc
    12061 agctgtagtt tcttctgttt cctcaaccag caataaagtt caatagaaag gatggagacc
    12121 aacattccca aaaataaccc tttcgtatct gtatattgtt tagccaatac gttttctact
    12181 ataacagtct gattttcatt cactaattca taaacagttg gattaacaga taaataagac
    12241 atcactgcga tgataccagg gaatacacca tccactttat tcagcttggc taattcgatc
    12301 cctatcagaa aaacagcata gatggtcaag aaacttagtg tgacatagct gatggcagat
    12361 gcgataggct ttaattcttc taaaaaacgc aagaaactta tctgtgctaa gccattttcc
    12421 gagtcaaaga ccattgctga aaacaatgtg ccaaaagcac ccgtaataat taccggaatc
    12481 aatgcagcaa atgcgttttt tatcaccatg atatacctta aattattgat tctcgttgcc
    12541 acgttcccta aaacattggc taatttatcc ataaaactca ttccagatcc cctcattcct
    12601 gcttcttctc cttgacgtca tctctttttg tctattagtc agcgcttaca tttcgatgaa
    12661 cagaaactat ttactcctct tttttaccaa atataaaaca aaaattcatc tgatgtattt
    12721 atcataaaat acatcagatg aactgtcaat tagtttcttt ttctcctatt attttctgta
    12781 attctctccg atttattgcc atatggacag tcattgcatc aaacgcagca agtgcattcc
    12841 gcctttcgat ggcctgaacg atctctgtat gaatatcgat cgtctcgatt ttcagatgct
    12901 ggatatcata atttttatta aacaaagcaa ttgattcatt aatgattggg ataatatgca
    12961 gcaaggctac gtttccgcta gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa
    13021 catgttcttg tgtgccttct cgaaaagaag tttcaatctt ttgcgttaat tttttcattt
    13081 ggacgatttg attcttcgtt gcatattccg ctgccaaacc tgccatctta ggctccaata
    13141 aatatctcag ttcaaataag tctcgtatca tccgatcctg atcttcaatc aaagaaaaac
    13201 cgagtgggtc attggcaatc cctcgcttat cactgacaaa tgtccccgct ccttgccgaa
    13261 cttcaaggat attcctggaa gctagagccc gcacagcttc tcgcaccgtg cttcgcccca
    13321 cctctaaata atcggacaat tcatattcat tcggcagttt atctcctact tgcaactgca
    13381 tttcctttaa atatctaagg atcttgtcag tagttacttc tactagggtt ttattttctt
    13441 ccaaaccaca tacctccttt tcatctgatg tattttttcg taattattat aacacgtttg
    13501 aatcatctga tgattttttg tatacaaaat tgattaatcc gttcatagac atacaaacga
    13561 agaagatatt taccataaaa aagactagga taaaattcac tcctagcctt cgatcatttt
    13621 tattttgaac gacaaaaaat atgatgttca ataacagacg atcttcattc gattcttgat
    13681 gtcatctttt gtttttttta atatatggat aaaaagtttg attacatcgt ttctattcta
    13741 gaatttggtc gattctcaat tcttcttctg gtgagaaatc taaattatcc atcattcgaa
    13801 cactttcttg taactgactt aaccgacttg ctccgaccag tacacttgta acagattttt
    13861 gtcgcaaatt ccatgcaagg gccatttgcg ctaatgattg tcctcgtgat acggcgattg
    13921 tttgcagtgc tttgattttt tccagagttg atccaacttg ttcttctgaa aggaatggta
    13981 tttccttacg atgtgctcta gaatcttcag gaataccatg tagataacga tttgtcagca
    14041 acccttgagc aagcggactg aacgtgattg cccctagccc ttcttcttct aatactggag
    14101 atagatcgtc ttctagtagt gcacgagaaa acatattgta gcgcatctga tgaataatga
    14161 acggtgcttc ctttcttttt aaaatttcag tcattttttt tgtatcttct ccattatagt
    14221 tggatatacc aatatataat gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg
    14281 tttcttccaa aggtgtatca gggtctgggc gatgatgata gaagatgtca acataatcta
    14341 gccccattct ttggagactt tggtcacaac ttgaaatgat ggatttacga gagccccatt
    14401 ccccatatgg cccttcccac atatgataac ctgctttgct agaaataatc agttcatcac
    14461 ggtaggcttg aaaatcttct ctaaaaatac gcccaaagtt tttctctgca gctccagcag
    14521 ggcctccata attattcgca aggtcgaaat gggtaattcc catgtcgaat gcgcctctta
    14581 aaatctctct ttgattatgg ataggatcat aatcaccaaa attttgccat aatccgagag
    14641 ataaaatcgg taatttcaaa cctgaatgac cggatttacg atattccatt ttttcataac
    14701 gtgcactatc tgcaatatac aatttttctc ctcctcatca aatcttttta aaacgctttc
    14761 ttttttctat ttttagttta ctacatttta aagaaaagga gttaatcaaa atgatttttt
    14821 cgtatatttt tttgccattt gttttttact cgttgataga tcagaaattg attacgtgct
    14881 tgctggattt ttttataacg tttcggttgt tttttgtaaa attgctgatg atagttttct
    14941 gctggccaaa acgtaccggc tggttcgatt tttgtgacga ttggttggtg gaaagttcct
    15001 gaactgtcta gtttctgttt tgattgttcc gctaattctt tttgtcgttc gacttgataa
    15061 aaaatcactg gacgatattg cttccctcta tcttgaaatt gtccaaatgt atctgtggga
    15121 tcagtaattt gccaatagat tgtcagcaac tcgctataac ttatttctct tgtgtcataa
    15181 atgatctcca cagcttccac atgacctgtg taaccaccac ttacttgatc ataatttggt
    15241 ttttcaacat gtccgcccgt gtatcctgac aaaacagata ggatcccttt tttcgattca
    15301 aacggctcga ccatacacca gaaacatccc cctgcaaata ctgcacgttc ttgatagtca
    15361 gcatcttgca tggcatgctt ggaaaagtca tatttttgct ccttatcaaa tccttccgta
    15421 atcaataaat aaaagtccat tacatcaggc gtcagattca ttcggatagc taatggacgt
    15481 agtgttgctt ctagttttga taactgttct ttaacggata tattttctct tatgtcattc
    15541 tttgtctgaa tcaatacttt tcgttcccaa tctcgtgtat tcggattcaa aatcagatta
    15601 taaagtgttt gaatgaatat ttgttcctca tccatagatt ctcctccttt ccgaagaaga
    15661 caagctagtg actttagctg taatttatca taaagtgagg aaataagcga ttacttcatc
    15721 cccctacaga tttttcgtat aaaaaagaga gaatccttat cgacagattc tcctttttaa
    15781 tcaatttatt ttataaatga aaagttaatt taccttttat ggaaaactat tttaaaaaat
    15841 aaacataaat atgcagttaa tgtaacaaga taagaaataa tagtcgttac tgtataactt
    15901 gtgcttactt taatatcagc aactcctttg actccgctta tttgtaccaa tccattagca
    15961 ttcacctgct cctttacagg ttgatgattt atttctacat tcgttcctaa ataagcatat
    16021 acaggaagtg tgattacttg gctcttctca ttagttactc tatatatgat agaactacca
    16081 gttgttttat aaggaaggtt tacccattga ctgccaatct ttgcttcgtg ttttattata
    16141 tgctccttat tctccaatga agcgatagga gaataatcat ataaaaaata atccattcct
    16201 ttagtggtat acgcctctac tgtttctttg tttacaacaa tatgtcccgg ctgagaaatt
    16261 attttttgat t
//
LOCUS       DAHUWM010000049        16271 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHUWM010000049 DAHUWM010000000
VERSION     DAHUWM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.049"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..622)
                     /locus_tag="NK788_001082"
     CDS             complement(<1..622)
                     /locus_tag="NK788_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769425.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPE"
     gene            complement(801..1520)
                     /locus_tag="NK788_001083"
     CDS             complement(801..1520)
                     /locus_tag="NK788_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6769426.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1651..2802)
                     /locus_tag="NK788_001084"
     CDS             complement(1651..2802)
                     /locus_tag="NK788_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCH6769427.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3019..3759)
                     /locus_tag="NK788_001085"
     CDS             complement(3019..3759)
                     /locus_tag="NK788_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6769428.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="NK788_001086"
     CDS             complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="NK788_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCH6769429.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="NK788_001087"
     CDS             complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="NK788_001087"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCH6769430.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4720..5370)
                     /locus_tag="NK788_001088"
     CDS             complement(4720..5370)
                     /locus_tag="NK788_001088"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCH6769431.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="NK788_001089"
     CDS             complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="NK788_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCH6769432.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="NK788_001090"
     CDS             complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="NK788_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCH6769433.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6813..7340)
                     /locus_tag="NK788_001091"
     CDS             complement(6813..7340)
                     /locus_tag="NK788_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCH6769434.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7520..8305)
                     /locus_tag="NK788_001092"
     CDS             complement(7520..8305)
                     /locus_tag="NK788_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCH6769435.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8298..9167)
                     /locus_tag="NK788_001093"
     CDS             complement(8298..9167)
                     /locus_tag="NK788_001093"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCH6769436.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9168..10547)
                     /locus_tag="NK788_001094"
     CDS             complement(9168..10547)
                     /locus_tag="NK788_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCH6769437.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="NK788_001095"
     CDS             complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="NK788_001095"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCH6769438.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10999..11475)
                     /gene="accB"
                     /locus_tag="NK788_001096"
     CDS             complement(10999..11475)
                     /gene="accB"
                     /locus_tag="NK788_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCH6769439.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="NK788_001097"
     CDS             complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="NK788_001097"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCH6769440.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="NK788_001098"
     CDS             complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="NK788_001098"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCH6769441.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="NK788_001099"
     CDS             complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="NK788_001099"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCH6769442.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14505..14729)
                     /locus_tag="NK788_001100"
     CDS             complement(14505..14729)
                     /locus_tag="NK788_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCH6769443.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14782..15744)
                     /locus_tag="NK788_001101"
     CDS             complement(14782..15744)
                     /locus_tag="NK788_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCH6769444.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15754..16194)
                     /locus_tag="NK788_001102"
     CDS             complement(15754..16194)
                     /locus_tag="NK788_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6769445.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4285 a         3712 c         2787 g         5487 t
ORIGIN      
        1 tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg
       61 gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt
      121 aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc
      181 cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt
      241 ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc
      301 tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg
      361 ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg
      421 gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc
      481 ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag
      541 ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt
      601 ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt
      661 tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac
      721 acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac
      781 ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca
      841 tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat
      901 agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct
      961 ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt
     1021 tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg
     1081 atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa
     1141 accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct
     1201 gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca
     1261 aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt
     1321 gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt
     1381 agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc
     1441 ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt tgctacgata
     1501 tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca
     1561 aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt
     1621 ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt
     1681 ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc
     1741 ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa
     1801 gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac
     1861 aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt
     1921 ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc
     1981 agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc
     2041 cagcaaagta gaagataaca accgataacg caaatacggc caataagatt cccaagtctg
     2101 cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt
     2161 tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat
     2221 tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata
     2281 cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag
     2341 cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt
     2401 ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg
     2461 tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca
     2521 gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc
     2581 ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg
     2641 atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc
     2701 aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt
     2761 gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag
     2821 ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg
     2881 aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga
     2941 gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa
     3001 ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc
     3061 tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc
     3121 cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg
     3181 aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct
     3241 gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg
     3301 atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa
     3361 tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct
     3421 atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc
     3481 tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg
     3541 ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc
     3601 ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt
     3661 gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat
     3721 cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact
     3781 aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg
     3841 cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc
     3901 ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc
     3961 tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa
     4021 caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc
     4081 ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct aatatgagca
     4141 gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt
     4201 tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga
     4261 tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata
     4321 tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc
     4381 acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa
     4441 gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg
     4501 tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc
     4561 aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct
     4621 gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt
     4681 tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat attcatacaa
     4741 cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc
     4801 ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca
     4861 aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac
     4921 catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc
     4981 gataataaag taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata
     5041 ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc
     5101 ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc
     5161 aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc
     5221 agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat
     5281 ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg
     5341 aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt
     5401 tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat
     5461 gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat
     5521 ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc
     5581 aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag
     5641 atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct
     5701 tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc
     5761 aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt
     5821 ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg
     5881 aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt
     5941 cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac gattgccgct
     6001 gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata
     6061 aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta
     6121 gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc
     6181 taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc
     6241 ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt
     6301 tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc
     6361 cgtcatctca tgagatttct tagcactagt taataataca tctactggac gaattcctgc
     6421 ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa
     6481 tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc
     6541 tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt
     6601 ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt
     6661 ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc
     6721 tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac
     6781 ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata
     6841 tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt
     6901 gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga
     6961 ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct
     7021 aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca
     7081 acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa
     7141 acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct
     7201 gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg
     7261 atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt
     7321 ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag
     7381 tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc
     7441 gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa
     7501 atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt
     7561 tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt
     7621 gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa
     7681 cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg
     7741 cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa
     7801 tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc
     7861 caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat
     7921 ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg
     7981 ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta
     8041 aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca
     8101 gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc
     8161 caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat
     8221 cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa
     8281 cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga
     8341 agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga
     8401 gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg
     8461 atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct
     8521 tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa
     8581 aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga
     8641 gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga
     8701 ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata
     8761 cttcccatga taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt
     8821 gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt
     8881 tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca
     8941 aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat
     9001 ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa
     9061 tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct
     9121 ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct
     9181 atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa
     9241 tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg
     9301 attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc
     9361 gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat
     9421 cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt
     9481 tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat
     9541 tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct
     9601 gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt
     9661 tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga
     9721 aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct
     9781 cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt
     9841 tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga
     9901 tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata
     9961 tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc
    10021 tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc
    10081 atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca
    10141 ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc
    10201 ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct
    10261 tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct
    10321 tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg
    10381 gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca
    10441 tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata
    10501 cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc
    10561 aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt
    10621 gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc
    10681 cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc
    10741 cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat
    10801 caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat
    10861 cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat
    10921 caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt
    10981 ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac
    11041 aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc
    11101 attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc
    11161 tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg
    11221 cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc
    11281 agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt
    11341 ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc
    11401 ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat
    11461 ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg
    11521 ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg
    11581 acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat
    11641 tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa
    11701 tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg
    11761 atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg
    11821 atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta
    11881 gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg
    11941 tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca
    12001 agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga
    12061 atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca
    12121 atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca
    12181 tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg
    12241 atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg
    12301 gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga
    12361 ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac
    12421 cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg
    12481 tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta
    12541 tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat
    12601 ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg
    12661 tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg
    12721 ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg
    12781 atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa
    12841 cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc
    12901 atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct
    12961 tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc ataatttgct
    13021 tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc
    13081 tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata
    13141 tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt
    13201 ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt
    13261 tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat
    13321 gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt
    13381 gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg
    13441 ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt
    13501 catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca
    13561 taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt
    13621 ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc
    13681 ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag
    13741 gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa
    13801 tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt
    13861 ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag
    13921 gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct
    13981 cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag
    14041 cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg
    14101 cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga
    14161 tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct
    14221 gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt
    14281 agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat
    14341 cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt
    14401 ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat
    14461 tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt
    14521 tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg
    14581 tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag
    14641 tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg
    14701 atgatttctt gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt
    14761 ttatttttat aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc
    14821 gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac
    14881 tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt
    14941 ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag
    15001 gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt
    15061 gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata
    15121 cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa
    15181 tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt
    15241 cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga
    15301 ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca
    15361 gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat
    15421 atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg
    15481 tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag
    15541 atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt
    15601 tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc
    15661 catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc
    15721 agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg
    15781 caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat
    15841 gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt
    15901 cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc
    15961 tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt
    16021 ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc
    16081 tgtgattgac agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat
    16141 atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca
    16201 ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt
    16261 ttcaaaatgc a
//
LOCUS       DAHUWM010000050        16195 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHUWM010000050 DAHUWM010000000
VERSION     DAHUWM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.050"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(369..995)
                     /locus_tag="NK788_001103"
     CDS             complement(369..995)
                     /locus_tag="NK788_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769446.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="NK788_001104"
     CDS             complement(1290..1625)
                     /locus_tag="NK788_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCH6769447.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="NK788_001105"
     CDS             complement(1612..1896)
                     /locus_tag="NK788_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCH6769448.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="NK788_001106"
     CDS             complement(1952..3475)
                     /locus_tag="NK788_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCH6769449.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="NK788_001107"
     CDS             complement(3468..4643)
                     /locus_tag="NK788_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCH6769450.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="NK788_001108"
     CDS             complement(4647..4832)
                     /locus_tag="NK788_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769451.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="NK788_001109"
     CDS             complement(4798..6492)
                     /locus_tag="NK788_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCH6769452.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="NK788_001110"
     CDS             complement(6489..6962)
                     /locus_tag="NK788_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCH6769453.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="NK788_001111"
     CDS             complement(7031..7186)
                     /locus_tag="NK788_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HCH6769454.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="NK788_001112"
     CDS             complement(7320..7700)
                     /locus_tag="NK788_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCH6769455.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="NK788_001113"
     CDS             complement(7704..7919)
                     /locus_tag="NK788_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769456.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="NK788_001114"
     CDS             complement(7922..8329)
                     /locus_tag="NK788_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HCH6769457.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="NK788_001115"
     CDS             complement(8595..10070)
                     /locus_tag="NK788_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCH6769458.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="NK788_001116"
     CDS             complement(10060..10908)
                     /locus_tag="NK788_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HCH6769459.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="NK788_001117"
     CDS             complement(10945..11307)
                     /locus_tag="NK788_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769460.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="NK788_001118"
     CDS             complement(11308..11466)
                     /locus_tag="NK788_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769461.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="NK788_001119"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="NK788_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="NK788_001120"
     CDS             complement(12228..12539)
                     /locus_tag="NK788_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769462.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="NK788_001121"
     CDS             complement(12583..12876)
                     /locus_tag="NK788_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769463.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13071..13718
                     /locus_tag="NK788_001122"
     CDS             13071..13718
                     /locus_tag="NK788_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6769464.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13779..14924
                     /locus_tag="NK788_001123"
     CDS             13779..14924
                     /locus_tag="NK788_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6769465.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15005..15310)
                     /locus_tag="NK788_001124"
     CDS             complement(15005..15310)
                     /locus_tag="NK788_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCH6769466.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15335..15766)
                     /gene="ruvX"
                     /locus_tag="NK788_001125"
     CDS             complement(15335..15766)
                     /gene="ruvX"
                     /locus_tag="NK788_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCH6769467.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15763..16029)
                     /locus_tag="NK788_001126"
     CDS             complement(15763..16029)
                     /locus_tag="NK788_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCH6769468.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT         4638 a         3358 c         2581 g         5618 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atccccccct cgttagcata
    12901 catttctata caaagatttt aaaaaaatgc aagaggctct tgaatacatt tctatacctc
    12961 ttataaactc aatataccat catacctttt catttgcaag aattttaaca tacttttttg
    13021 aacttttatg tttcacacat atttaaatcg tgatatagta gagaaaatat atgaatggtg
    13081 gtgaaaatgt ggctagaaaa aaataccccg aagcagtgga gacaggacaa agaattagac
    13141 tggctagaat tgaaaaggga ctctcaatgg aacaacttgg aggtaaatta tccccaccgg
    13201 ccagtaaagg tgccgttagt aattgggaaa atggctataa tcttcccaat aacaccaggc
    13261 ttaaacaact ttcaaaggta ttagatgtat ccactaccta tctattaaac ggatcttata
    13321 cattaagaga tatgcatatg atgcccgaaa ttaagcgaaa tgtgcttgaa aagcaattga
    13381 aaaaaactat tgaaaaaaat aactctataa ttgattctaa tctttctaat gctatcaata
    13441 acatcgaaga agataacctg actgtctcac agaagattta tttaacaaac gcaataaaat
    13501 atcttaaaac aggatctcga attgaatttc ctgacgggag taccccaatt gcttatctcg
    13561 cagctcttct aaatacgctt ttacgatatg actcattaat taatgattca gaaaaaaata
    13621 cagatgagaa aaaagagtat caagaatttt atgaaaatga tcttctaaaa gagttcacag
    13681 ctcttgttac tgagctaaat aaacactatt caaaataata ataaactgcc tcctccaacc
    13741 gcccccacgg cttgctacac tatggaggga aaactattat ggcaacattc aagcaatatg
    13801 aaactaaaaa aggtaaattt tggctctatg aagcctatct tggaatgaac aaaatgactg
    13861 gcaaacctga taaggttcgt agacgaggtt acaaaacaaa aaaagaagcc cagcttgcct
    13921 tgtcacgctt gcaagtggac tacgataaaa acggtattaa aaaagcgaac aacgaaacgt
    13981 ttaaagaagt ttatgacttg tggctagaaa catataaaac cactgtccga gaagccacct
    14041 tcatgaaaac tgaaatcaag tttagaaagt ggattcttcc aaaatatgga aatcttcgtg
    14101 tgaatgaagt cacggtaaaa aaagcccaac aaattgtaaa tagctgggct gaaactactg
    14161 accaatacaa ggtacttcat tcaacttcta agcgtatttt tagctacgct atcaatcttg
    14221 ggattattga gactaatcca ctagacaacg tattaatgcc taaaagaacc accactaagc
    14281 ataaagaaaa ggtcaaagtt tacagtaaag aacaactgat caccttgttt gattacctaa
    14341 acagcaaacc tgaaacttat cgaaacgact ttgataaaac gttactacgc tttctctttt
    14401 atagcggggt taggatcagt gaagcccttg cattgaattg gtcagacatt gattttcaag
    14461 aaaatacggt caccatcaat aaaacactaa gccaatccaa aaatggctat aaaatcagtg
    14521 atcctaaaac tgattgtagt gctggggttc ttccactaga tgaaagaaca atttctatat
    14581 taaaaaaatg gcagattaat cagcggaaat atatgctgac acttggtgta actgatccga
    14641 ctatgatttt ttgtggtatc tataaagaac taatcacaca ccacgctatt tatgcaagat
    14701 tgattactat ttgtgaaaaa gctatggttc catttctcgg tgtgcatgta gcaagacaca
    14761 ctcatgcttc aatgctcttg gacgctggag ctactatggt agaagttcaa actagattac
    14821 ggcatacaaa aattagtcag acgatcgatt gttacggtca cttggcaaaa gaaacgaaag
    14881 aaaagactgt tgaaaaatta gtacaacact taaatattga ctagatggca tacaatatgg
    14941 catacagtaa aaaataaaaa aaggagaaac cctatcatat caaggtttct cctagcttta
    15001 tagtctattc ttcttcttcc gccatgaatg tattgaatac ttcttcaatc atgtcccatt
    15061 ctgcgtctgt ttcgatttgt tctaactcgc cttctgttcc atcttcgttt tcgatgtatg
    15121 cataagcttg taattctaca tcttcatctt ctgaaacacc agcaggatat aaaagaacat
    15181 agttacgtcc aaattcttct tgtccgtcaa ctgttagtag gatttcatat aatgtttcat
    15241 ttccttggtc atccaccaat gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat
    15301 gttctgtcat aagattacct cttttctttt attattaaaa taattgctta cctgagccgt
    15361 ctaaataatt ttgtaagatc atcacagctg ctaatttatc gatgactttt tttcgtttgg
    15421 ctcttgaagt atttgcctgc tctactagca tacgttctgc ctgaacagtg gttaatcgtt
    15481 catcttgata atcaactggc aaggagaata gttctttgat cttttctccg tatgacatgg
    15541 aagcttctgc ccttggtccg atggaattgt tcatattttt aggtaatcca actacaaacc
    15601 ggtctacttc atattgtgct actaattcct tcaaccgatc aaagccaaac tcttcttctg
    15661 cttcattgat ccgaatgatc tctactcctt gagctgtcca gccaaagggg tcactgatag
    15721 caacacctac tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg
    15781 atttgagagg tagtccttga caattacttc catgatctca tcgcgttcgt gacggcgaat
    15841 caaatttcga gcatcacgat aacgaggaat gtaggccggg tctccagaaa gcaagtagcc
    15901 gacaatctga ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa
    15961 tgtttcgctc acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt
    16021 aaaacccatg attctacacc tctttcccgc aaggtaattc cagcttacct tctacaacct
    16081 tattttactc gaagaaaggg aaaactacaa acaaatattc caaacttccg aataaattaa
    16141 gaaacactat ttgtatgttt ttttcctttt ttctgtagct ttatcaaaag tactc
//
LOCUS       DAHUWM010000051        16158 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHUWM010000051 DAHUWM010000000
VERSION     DAHUWM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.051"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(283..1167)
                     /gene="rsmA"
                     /locus_tag="NK788_001127"
     CDS             complement(283..1167)
                     /gene="rsmA"
                     /locus_tag="NK788_001127"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCH6769469.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(1160..1729)
                     /gene="rnmV"
                     /locus_tag="NK788_001128"
     CDS             complement(1160..1729)
                     /gene="rnmV"
                     /locus_tag="NK788_001128"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCH6769470.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1722..2498)
                     /locus_tag="NK788_001129"
     CDS             complement(1722..2498)
                     /locus_tag="NK788_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCH6769471.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2653..3975)
                     /locus_tag="NK788_001130"
     CDS             complement(2653..3975)
                     /locus_tag="NK788_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6769472.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            4150..4737
                     /locus_tag="NK788_001131"
     CDS             4150..4737
                     /locus_tag="NK788_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769473.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(5216..7225)
                     /gene="metG"
                     /locus_tag="NK788_001132"
     CDS             complement(5216..7225)
                     /gene="metG"
                     /locus_tag="NK788_001132"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCH6769474.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7493..7771
                     /locus_tag="NK788_001133"
     CDS             7493..7771
                     /locus_tag="NK788_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769475.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7868..8689
                     /locus_tag="NK788_001134"
     CDS             7868..8689
                     /locus_tag="NK788_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCH6769476.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8974..9984)
                     /locus_tag="NK788_001135"
     CDS             complement(8974..9984)
                     /locus_tag="NK788_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6769477.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9996..10949)
                     /locus_tag="NK788_001136"
     CDS             complement(9996..10949)
                     /locus_tag="NK788_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6769478.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10939..12009)
                     /locus_tag="NK788_001137"
     CDS             complement(10939..12009)
                     /locus_tag="NK788_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769479.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(12015..13058)
                     /locus_tag="NK788_001138"
     CDS             complement(12015..13058)
                     /locus_tag="NK788_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769480.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(13059..14000)
                     /locus_tag="NK788_001139"
     CDS             complement(13059..14000)
                     /locus_tag="NK788_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769481.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            14084..14431
                     /locus_tag="NK788_001140"
     CDS             14084..14431
                     /locus_tag="NK788_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCH6769482.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14501..>16158)
                     /locus_tag="NK788_001141"
     CDS             complement(14501..>16158)
                     /locus_tag="NK788_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6769483.1"
                     /translation="KKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFNLV
                     VPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTYKI
                     KLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKSEL
                     GIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNGPF
                     TLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDVIL
                     SGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVKQV
                     LGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDSLE
                     FDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGGWA
                     ADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIKII
                     SDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4553 a         3343 c         2908 g         5354 t
ORIGIN      
        1 ttgtgtcaca tctccttgtt cggcataata gtggtcaaaa gcttgcatca agtaataaga
       61 ccaactaact caaaaacatg agaagctgtt ttttgaaatt gttccttgtg acttagagtt
      121 aaacggcagg cccacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaac
      181 aagcccaaac aaacgaaaaa catgaggaac tatttttgtt tggctgttct tattacttga
      241 tgcaggacag ctttttcaca acttctgtca caacctcttt ttttactgca tattttctga
      301 catcgcattg cttaatgcgg cgaattcttg taatgacaaa gtctccccac gacgttttgg
      361 atcgattcca gctgtttcca agctttttgc taaccatgct tttgtctggt catctttgcc
      421 atacgaatgc tgcaagttat tccaaagcgt ttttcttctt tgctgaaatg ccgcttttgt
      481 caatcggaaa aatgcttttt cgtctgtcac ttcgactgcc ggtatatctc ttcttgtcag
      541 tttcaaaatc gctgaatcta catttggctg agggacaaat actgtttttg gtacgatgaa
      601 tgctaaactg gcttccatat aatattgaac agcaatcgat aatgaaccat aagctttcgt
      661 tcctggttca gcagagatac gatcagctac ttctttttgc atcatgacga ctagttcatc
      721 cacaaccaaa tctgattcaa gaaaatgcat catgatcggt gtcgtaatat agtaaggtaa
      781 attcgctacg acttttaacg gaagctcctc atgaaatgtt tctctaacag ctgttgacaa
      841 atcggtcttt aaaatatctt gatgtacaat agtcacattg tcatagggtt gcatcgtatc
      901 agccaataca gggatcaaac gatcatcgat ctcaaatgct acgacttgtt ttgcatgctt
      961 ggctaattgt tccgtcaatg caccgattcc tggtccaact tcgatcacgt tcgtatgttc
     1021 atctatttgc gcagtttcta cgattttcct taaaatattt ggttcagtca gaaaattttg
     1081 tcctaaactt tttttaaatg aaaaaccatg tttcaataaa atctcttttg ttctagaggg
     1141 tgtcgcaatt tcacgatatt cactcatgtt gttcctccac tattttcatt gcttgggcga
     1201 gttctttcgg cgtaatacga aacatcttca aacgtttttc caattgtttg ccgttcgtgt
     1261 aaccgatgcg caactcgtca cctagttttt ctcgtttttc ttttgcttgt gccccagcta
     1321 ttaaaccata gtctaaaagg tcttgtctag tgatttcaga aacagccgtt tgggaaatgt
     1381 ctggcgtcac gacctttttc aatgcttcta aaatagcagc atcacttgca tgctcgacac
     1441 caaggcttcc ctttttcttt ggtttagctt gtgtgcgcgg caaaaaagca tgttttgcct
     1501 gaggaacagc ttccataatg atttttctta ttttttctcc tgaaaaatca ggatcggtaa
     1561 aaacgatcac tccacgagtc tcttgggcat gttcaatttg catcaggatc tcttcattga
     1621 ttgcagaccc tattgtttct atcgtatctg catcaactac ttcccgcagc cttcttgtat
     1681 cgtcttttcc ttcaacgact ataatctctt ggatcttcat cttatccatg ttcgcttaga
     1741 ccaaataatc ggtgcgcatt tgtccatgtt tgggcagcca cttcttctgt cggaagattc
     1801 cgaagttcag caatcttttc tactgtataa cgagtatagc ctggttcatt tcgctttcct
     1861 ctaaaaggaa ctggtgccag ataaggggca tctgtttcca caagtaatcg ttcgattggt
     1921 acatttgctg cagcctcttg gacgtctaat gcttttttga aagtgacgac accgctaaat
     1981 gaaatatgca tccctaagtc aagaaatcgt tttgcccatt cataatcacc actgaaacta
     2041 tgcatgattc caccgatatc tcgaacatct tcttctttta ggatttgata cgtgtcttca
     2101 agtgcttctc tagtatgaat actgattggt aggttcattt cttttgcgat agcaatctgt
     2161 ctgcgaaata ctttatcttg gacttctttc gggtcttcca tccaatgata atccaagcca
     2221 atttccccta atgctacaac ctttggtaaa gtcaattgtt cttgtaattt attttcgatt
     2281 tctttcgtat aactgccagc ctctgtcgga tgccagccga taatactgta tatatttgga
     2341 tagtcatggc ttaattgcaa agacttttca atcgttggcg tatcgaagcc cacgaccgcc
     2401 attttcgtta cccccatttc ttgtgctcgc tgaatcgttt ctggaatatc ttcattgaac
     2461 tgttcagcat ttaaatgggt atgagaatca aatatcatgt ttatctctcc ttttcattct
     2521 tactttagca cataagcact acttgcagaa gtagcaaaaa tcaaaataga agaacttgtt
     2581 ttattgaaat agaaaaagca cgaagatcta gaggacctag ttcgtacttt ttctgtctgt
     2641 tgttgaaata ttttactctt ctgagcgttt tttctttttt tgataataga ctgggatacc
     2701 tatcaaggta acacctatac cgatcaatgc taatttcgtt tgcgtcatca tcgtcatacc
     2761 taaaatgaaa atagcaccga gaatcgcaat aatcggaacg attggataaa gtggtacttt
     2821 atatggacgt ggcaattccg gcgctttttt tcttaaaata aagacaccga taaaaatcag
     2881 taaactgaat agccacatca caaaaatcaa catgtctgtt aaaaagtcga aagaaccaag
     2941 gctcatcata atacaagcta ctgctaactg gaaaaccgca ggaacataag gaaccgtaaa
     3001 cttctttgaa agctttgtca gctgtttact gaaaggtagc tcgtcttcta aagccatcgc
     3061 ataaggcacg cggatcccag ttagtgtata accgttcaat gcaccgtata ccgagatcaa
     3121 gatcccaata gtgaccagct ttccaccgat tcctccaaaa atgacgtctg atgcttcaga
     3181 agctgcattc aaattaccgg caagatgatc aatcggcaaa gttttcaaaa aaacaaaatt
     3241 gatcaataga taaacgactg ttacaaaact caaaccaaga ataattgctt taggcaaatc
     3301 tttttccggc cgtttcattt cccctgccat tgcaccgaca cctaaccagc cgtcataagc
     3361 aaacaacgtt gctaacaacg cactgctcaa tccttcgaca aaagtaacat cttttccagc
     3421 ttcgattgga aataactgga cagttccttg tcctggggtc aataatcccc atatgacaat
     3481 cacagcaatt ggtattaatt taacaatcaa cgtcactgat tgtacagaag caccgacttt
     3541 tgtccctaac aaattcaacc ctgtcacaga aacagcggtg atcactgcaa tcaacaaaag
     3601 ataatcggta gataattgta ataagttcgt caattgtgtc gcaaagataa tcgataatgc
     3661 agcaatatta gcaggaaagt aaatcaagct ttgcgcccat cctaataaaa agctgggcaa
     3721 ttttccatac gcagcttcaa tatattttac agctccacca gtttccggga ttgctgtagc
     3781 taattcagcc actgtcaatc cagcacagat cgttaagaaa ccacccaaaa gccaagctag
     3841 caatgtcaaa cttgtagact gagtactagc agtaacagca gcagttttga agaagacgcc
     3901 tgcaccgatc acggttccca taacggtagc taatgcgcca aacagattga tttctcgttt
     3961 tagtttgcct ttcattccca tcctctttct tctttgaaat ttggtataat tttcagtata
     4021 actgagaata ttttaattta ccattctttt ttctccactt tcagtgaaaa actccctctt
     4081 aagacggatg tatttctttt ttatttttcg tatactaaac ctaccttttc cacgaaggag
     4141 gaacagttta tggattttaa gcgttcttat ccgaaattag cagattattt atccaaccgg
     4201 gtcgattttt cccgtaaaga acaacagcag tcccattggc attggttcac attaatttta
     4261 tttggattaa tgactgttgc aacgttatca caaaaactaa tattgctgac tttgttcatc
     4321 gcaatcgttg cattgatcaa aggtcctgtg atgatcttgt gggggatgtt gtattccttt
     4381 ttggttgggc tttttccgcc tatcggtatt ttgctttcag cgattttctt tttattgaat
     4441 atcggaacat ttacaaagaa ctggcggatg accttagtcg ggatctattt ttatttctat
     4501 ccttttggag tgatggcact tagtgaagtc atgcattggg ataatcattg gtttatagct
     4561 ggctccttgc ttttagggtt gatcttgtta cacgtgatgt tgacaaagct ctatcaacat
     4621 tatggtatcg gacgtacgat tttctggtat gtcttttcga tcccatttgc actgcttact
     4681 gctctcttac caagccgttt gaagacaaaa ataaaaggtt accataaaat caagtgatag
     4741 gatttttaca aaataaagaa aacaatcatc gaagtcaaag ccgatgattg tttttttatc
     4801 tatattttca atcacacata taagagcaca aactaattga acataaagtg atagaactta
     4861 taattaacac atcaacgcta ctgttagata taaatataga tgtaataaag tcattgcatt
     4921 caaactttat gaatgtaagg acaataaaaa agagtaccag atgaatcaat taaaatctgc
     4981 ccgacccttt atcattatgc tttttttctg tttttacgat tcctattagt attattctaa
     5041 attaacaaaa atcagcgaag ctcgtacttc tttttctagg atttattttg gatagttctc
     5101 tgtttctgta ttatatgatt ggcttcaagt aataagaagg aatttacgaa aattgcttct
     5161 caaatttcgg aaaaaggggg tatgacacaa ctagtgtcat accccctttg aatttctatg
     5221 cgatttctga gccatttggc atggattttg gtgcttcgat tacttgtaaa gagccatctg
     5281 gtgcttctgc agaaaggatc attccttgac tgatttgtcc acgcatcttt cgtggtttca
     5341 gatttgctac aatgacgact tttttcccga tcaattcgct tggatcagga taaaattcag
     5401 cgattcctga caagatttga cggtcttggc tgtctccagc atctagtcgg aattgcagca
     5461 acttatctgc cccttctact ttttgacagt tgatgacttc tgctactttt agttctactt
     5521 tttcaaatac atcaaatttg atttgttttt ctttcgttga aacaagttct gtttcttctg
     5581 gatcccattt gactgtatct tcgtttgttt gagtaccttc agacattttt tcttgaataa
     5641 aagcaacttc ttcttccata tctaaacgtg ggaagattgg tgtacctttt gcaactactt
     5701 ttgttcctga aggaaattcg ccaaagtgta atccttcaag attcattgtt tcagagtcca
     5761 atcctagttg attgaagatc ttctctggcg tttctgtcat gattggctga agtaatatcg
     5821 ctgcgatacg caaagattca gctaaatgga tcatgacact attcaattca gctttctttt
     5881 cttcgtcttt tgctaatacc catggttctg tttcatcaat gtatttgttt gcacgagaaa
     5941 tcagcaccca gatttcagcc aatgctgtat tgaattccat tttttccatt gcatcatggt
     6001 aacgtccgac aacgttagca gccgtcgtag acagttcact atcaaatgga gtgacaagtg
     6061 aggcatagtc aggaacgagt ccatcacaat atttgttgat catagcaatc gttcgattca
     6121 ataagttccc tagatcattt gccagatcat aattgacacg ggaaacaaaa tcttctggcg
     6181 taaagacacc atctgaccca aacggaacag cacgaagcaa ataataacgg agtgcatcta
     6241 aaccataacg ttccacaagc atttcaggat aaacgacatt tccttttgat ttagacattt
     6301 ttccgtcttt catcaatagc cagccatgcc caaatacttt tttaggtaga ggcaaatcaa
     6361 gggccatcag catgattggc caatagatcg tatgaaagcg aacgatttct ttaccgacca
     6421 tctgaacatc tgcaggccag tatttttcga atagactttc atcttcggag ccatatccta
     6481 gtgcagtaat ataattggat aatgcatcga tccatacata caccacatgt ttcggatcac
     6541 tcttgactgg tactccccaa gtaaaagttg ttcgtgaaac tgcaagatcc tctaatcctg
     6601 gtttgataaa gttgttgatc atttcgtttt tacgagattc tggctggatg aattcgggat
     6661 gttcttcata gtattccaat agacggtctg cgtatttgct catgcggaag aagtatgatt
     6721 cttcttttac caattctact tcgtgtccac ttggcgcttt accaccgatc acgttacctt
     6781 cgtcatcttt gtacacttct gcaagttgtg tttctgtaaa gaattcttcg tcagaaacag
     6841 agtaccagcc ttcatattca cctaagtaaa tatctccctg ttctaataaa cgatcaaata
     6901 ttttttgaac agctgcttta tggtaatcat ctgtcgtgcg aatgaattta tcataactga
     6961 tatcaagcgt tttccataat ttcttgacat cagccgccat tttatctaca tattcttgcg
     7021 gttttacacc tagttcttct gcttttgttt cgattttttg accatgttca tccactccag
     7081 tgagataaaa cacatcaaag cccatcaacc gtttataacg agcaattgcg tcacatgcaa
     7141 tcgtagtata cgagttgccg atatgaagtt tgccgcttgg ataatagatt ggcgtagtga
     7201 tataaaaagt ttccttttct gccacggggc tcttcctcct ttatcgcatg atgcgaattc
     7261 gtttccttgc tagtatacca taaaaaaagc atgctgttag ctttttttca aaatgaaaaa
     7321 gactggataa acgacttcta tctacgttca atctatcctt taaaaggatg aataggttga
     7381 ataaatgcgg gtttttgttt tgtttcatat ctactaaaaa acggcttttt atttctcccc
     7441 tcacacgtgg tataataaag actatctatc ttagtaaggg actggaacaa tcatgcagaa
     7501 gaaacatctt ttttttacac ttagtattgc ttttctttca ttagcacatt taatattcag
     7561 ctatttttat attcgtatgt atggttattt taacctccat gggcatttga acagttttat
     7621 gacagcagct tggattttgc gttttatcat tgacgtatat atcgttattt gtggcttctt
     7681 tgccattcgt gaagagcggt ataaagtatt gccgttttat ctgcttttct ttttattcaa
     7741 tctcatactt ccatttattt ttcatattta aatgataacc tagatttttc aagtaaatcc
     7801 ttggtttttt ttcagagtct gctacaataa ggtaaaaagt acttttactg tcaatggagg
     7861 tggtcagatg aaccgttttg cttctaaaga agaagaaaaa ttatattatg aacataaagc
     7921 aagtgaagaa gaatttctta actggtatca aaaacaggaa cgtccagaat atgacaagcc
     7981 ctctttgact gtcgatatcg tactgatgtg ctataacaaa gaagaagatc agctgaaagt
     8041 tttgttgatc caaagaaaag ggcatcctta tcgaaattca tgggcattgc ctggtggctt
     8101 tgtccaaaag gatgagtcaa ctggagaaag cgtcttaaga gaaacaaagg aagaaactgg
     8161 tgtggttatt tcaaaagaaa atattgagca actgcataca ttcagtacgc ctaatcgaga
     8221 tccgcgaggc tgggtcgtca cagtcagcta cttagccttt attggagaag aaccgctaat
     8281 cgccggagat gatgccaagg aagtacgctg gttcacacta gaacgtcata gtaacaagat
     8341 ccatttatct agcggagaag tggctatctc gcttgattta gtcactggag agtcttctgg
     8401 caaagatact ttggcttttg accatagtca aatcatcttg aaagcattca accgtgtagt
     8461 caataaaatg gaacacgagc cgcaagttct acaagtttta ggtaaagatt tcacgattac
     8521 ggaagctcga aaagtatttg ctaaattctt agggatcgat tataaaacga tcgatcattc
     8581 caattttaag aaagctatgc tccaatattt cgatgagatc ggtgaacgtc ctgtaggaat
     8641 cggccgtcct tcaaaaatct atcagctcaa gccggggcac catgaataag gttgtgagca
     8701 aaagattcag acctaatcat tcaagaaaaa acgcaaaaac aactctttgt gagttacgtt
     8761 ttttgtttca gaattttgat gtattcgtaa actttctgaa taaaaaatta acaatagcag
     8821 aaaaagctcg tacgaacttt ttctgctatt gttttattgg tttgtcataa ttagtgagct
     8881 tttcttcaaa agaaaaattc ctagaaacaa aagtcattaa gactttttat taacaaggct
     8941 aaccctaacg ctcctatcgg tttcattcat tttttatccc ttgataaccg aatgtctgcc
     9001 cattttttct actacttggc tctttcttac ggaattgctt tcttctgctc cgtcggtatt
     9061 gatcaaaaga aattttttat tataccattg ggacttataa cgcaattttg ttattaattt
     9121 atgccagtcg tgaatagaaa aatcagcatg tgcctcttct tttggaaaag attctacaaa
     9181 taagtagtca tattgtttga ccaatttccc agataattgt tcaagataat cttctcgctg
     9241 gttcttttgt ttcagataaa gatccattac ttttctcttt tgtttctgat aattgcttgc
     9301 ctgatctaac ctgatctttc tatggtgagc ggagcggact ttcacattca atttccgctg
     9361 tgcatgttgc agcttttctt ctgtctggtg aaattttggt aaagttactt ctagaggtcg
     9421 agatgtttgg atcaatgtct tcggatgata ggcgatcccg atccatttag ctaaagattc
     9481 tttttttact ggcgaaacat cacaaagtag tgcgacataa aattcttcat tatatcgagc
     9541 agaaatcgta gcggagcgaa tcgatccttc aactggccga tgctgattta tcttgattag
     9601 ttctttttgt tttggtaact ttaagtagcc attttccaga tagatcgtac gggtttggtt
     9661 gttggtcgta taagattgcc acatactttt tttcgtttta agctttggga agctggcacg
     9721 tcccttgaaa taattgcgaa aagcccgatc caaattaagc tgtgcatttg ctaaagcaag
     9781 actgtctact tcttttaaga atgggtattc tttttttaat gtagcgggtg tgagcgaaaa
     9841 attggctact cctgtttgtt gccaactttt ctgccgcttt ttcagcaaat ggttataggt
     9901 aaatcgagca catccaaaag tacgaatcaa ttgctgtttt tgttcttctg tcggatacat
     9961 acggaattta aaagctttta gcatctatgt ccctcctagt tagcaaccag aagagattcc
    10021 ttttgtcgtt ttagtttttt tgcgtaatat gggacttcct gctcagttgc atagacaaaa
    10081 tgttctggtg cgatttcacg aagttgacgt acttgtccgt catctggttc aggttgatat
    10141 ttgattcttc tgcgttttct ctcatggtct ggatctggta gaggaatagc agataacaaa
    10201 ctttctgtat aaggatgaac accgtgatta tagatttcat cacttgttcc catctctaat
    10261 aacaagccgt tatgcattac accgattcgg tcactaatat gtttaaccat cgacaaatca
    10321 tgtgcaataa atagataggt caattgatgt tctttttgga gatcttgcaa caaattgacc
    10381 acttgggctt ggatcgacac atctagtgca gaaattggtt catcacaaat gataaaacgc
    10441 ggttttactg ccaatgctcg tgcaatacca atacgctggc gttgcccacc tgaaaattca
    10501 tgaggataac gagtaccgtg gcttggattc aatccaacag ttctcaatag atcatcgact
    10561 ttttttgcac gctcttcaga tgatttagct agtccattaa catcaattcc ttcggcaatg
    10621 atatctctga ctttcatacg aggattcaac gaagcgtatg gatcttgaaa gatcatctga
    10681 acatcatgcc ggaatttcgt caaacctttt ttatctttga tcttcgttat atcttgaccg
    10741 tcgaataata tctcaccatc agtaggttca ttcaagcgaa tgatcgtacg gccagtagtg
    10801 gattttccgc ttccagattc tccaactaaa ccgaatgttt ctccttcata aatgtgaaag
    10861 ctgatgtcat ttacagcatg tacttcattc ttttttccta cattgaaata ctgtttcaat
    10921 cctttaactt caagtagttt atttggcatc agcaattcct ccttgaccgt aagtatgttt
    10981 ttgtttctct ttaaagatag cccagcgacg ttggatttct tctggcggtg tcactttggg
    11041 tgcctgcgga gcaagtagcc aagttgctgc cttgtgtgtt tttgatagtt caaaaaatgg
    11101 tggttcttgt tctgcatcga ttttcatcgc gaattcattt cttggataga atgcgtctcc
    11161 ttttggcggg ttcaataaat ccggtggaga accaggaata gcatacaatc gatcgttcgc
    11221 actatctaat gtcggcattg aaccaagcag tccccaagta tagggatgtt gcgggttgta
    11281 gaaaatttct tccgatgttc caacttcaac gattcttcct ccatacatga cagcgacacg
    11341 atcagcgaca ttcgctacta caccaaggtc atgagtgata aagataattg atgtatcgat
    11401 ttttgtctga atttctttca gcagctccaa aatctgtgct tgaatagtca catcaagcgc
    11461 tgttgtaggt tcatcagcaa tcaggatttc cggataacat accagtgcaa tcgcgatcac
    11521 gatccgttga cgctgacctc ctgagaactg atgcggataa ctgttgatac gtttctcagc
    11581 ttcgggtatt ccaactaatt taagcaattc taatgcagct ttttgtccct ctttttttga
    11641 aactttatta tgctttctaa gggactcagc gacttgcttc ccaatgggca tcgtcggatc
    11701 taaagaggtc attggatctt ggaaaatcat cgctatttct ttaccacgga ttttttgcat
    11761 ctctctttct gattttccaa cgatatcgtt acctttaaag aggatttgac cagcatctat
    11821 attggcgttg cttgacagca agcgcatgat gcttctggtc gtcactgatt ttccacttcc
    11881 agattcccca acgatcgcca atgtttctcc tttattcaaa tgaaaattga caccgcgaat
    11941 cgctcgtacc tttccagcat acgtatcaaa agagatttct aagtctttca cttctaaaat
    12001 tttcgtcatc gtcttcactc tttcatttta ggatcaaagg catcacgcaa tccatcagca
    12061 agtaagttaa agccaatcat gataaccgat aaagttactg ccggaatcca taatagataa
    12121 ggcagataca ggaatgtctt gtacccttcg ctcaacatcg ttcctaatga agctgacgga
    12181 ggcgtaagac ctaaaccgat aaagcttaaa aaggcttcga agaaaatagc agatggaata
    12241 ctaaacatca tttgaataat aatgacacta gagatattcg gcaaaatatg tttgaatgca
    12301 attttcattc ttgattcacc taatgtcaat ccagctaaaa catattcttg atcttttaat
    12361 ttcattgttt gtgcacgaac gatacgtgcc attggtatcc agtttgtgat ggccatagca
    12421 gccacgattg aaaaaattcc tggttcaaaa acgaccaaca tcaaaatcat gacaacgagg
    12481 tttggaattc ctgataagat ttccaaaaaa cgttgcatca cgttatcgac acgtccgcct
    12541 aacaaaccag aaaccaaccc gtaagctaca ccaatcgtta catctagtaa tgcagcaata
    12601 aatgcgatca gcaacgagat tctcattccc ataaataagc ggcttaatac atcacgtccc
    12661 aaaccgtctg ttcctaaaaa gaaatttacg tttgaaggga cgtttgcttg agcatattta
    12721 tcaacaagtt caccaccaac cattgctttt ccattcaatc catcgatatt gattcccgga
    12781 atacgaggag gcaaattgat ataagccacg ttttgggcag ttggatcatg aggagaaaca
    12841 aagattgtga tcacagagat caaaatgatg accaatagta aaaccattga aataacggca
    12901 gctttgtttt tctttaaacg gcgccatgaa tcttggataa aattcaaaga aggtgcagta
    12961 atctgttcac gttcttcttc ggtatttttt tgtaatggaa gaaattcatc cgcagggata
    13021 tctgcaactg tttcataacg ctttggtatc atttccattt atcctcggct cccttctgaa
    13081 acacgaattc ttggatcaac tagaccgtac aatagatcca cgatcaaaat aacaaaaatc
    13141 agcataaatg agtaaaggat cgttacagcc atgatcgtag gataatcatt tgttgtgatt
    13201 gacttaacga attgttcccc gataccagga atagcaaaga tattttctac caccagtgat
    13261 ccggtcatca atgctacagc taacggtcct aataaagtca tcaatggaat caaactattt
    13321 cgaagaccat gtttaaaggc gatttcccaa cggctcaatc cttttgcttt tgctaattcc
    13381 acataatcac tatgcagtac ttccaccatt tccgtacgga taaaccgagc agagtcagca
    13441 agcggcgaca tggccaacgc aattgttggt aagattgtgt acacaaagcc atcccatttt
    13501 gcgattggca aaacttgtaa tttgattgca aagatatatt gtaataaaac agcaaataca
    13561 aagtttggaa tggaacgacc taatatcgct acgagtgtag aagatgtatc tgcccatgta
    13621 ttttgcttca tggcagagat cgttcctaaa atcgtcccta ggactgttcc aaagataatt
    13681 gcctgtaacc ctaactgcaa ggatggtccg attcttccag ctaacaaatg tgcgactggc
    13741 tggtttttaa attggaagga aattccaaaa tctccttgaa gcaaattaga aagataaatc
    13801 ccatattgta cgataactgg tttatccaaa ccgacttgtt tatttagcat ctcgattgtt
    13861 tcaggactca aacgttcctg attcgtatat ggcgtgcccg gcagcaattg catcaagaaa
    13921 aaggtgattg ttgcaattaa ccaaagcgtg atgatcatga aaaagacacg ttttaataca
    13981 tatttgatat aactattcac taatgtttcc ccctagcttt tgacttctga cagatgtttc
    14041 gctacattat tagataatga accaaaaata aaggagcaat caaatgaaaa aaaaatctat
    14101 cccctacgca gtagcttttc tactgattct ggttatttta ataaaaaatc tcatcaacca
    14161 ttcctttaca ctcattcaat tatctaatga tctattctta tggtcattgc cgtttctgat
    14221 tatcggcggt tttttatggg tcttttcttc gggtttcttc gatcattttc agcgatcggt
    14281 ccatcttgca cgaacaagga accgaaagaa aaaaccagaa ttttcctcgc tctcgtctgc
    14341 cagctacggt atgtacagct tttggctgat cattgccgga atactaattg ctctttctgc
    14401 tattttcatg cttttttctt tgttaggata agtgaatatg agaacgaggt tgcgacattt
    14461 gccgcagcct cgttttttct catgctcacg gtattaacga ttattcttca acgtatgtcc
    14521 atttgtaatc ccatgaagcg cctgccgcat ggtgaacgat acctttgatt ttttcattca
    14581 ctaggtgacc ttctgctttt tggtacaccg gaacgacacc catatcatca gagatgatct
    14641 tgattgcttt ttggaaatca tcccaacggg cttgttcatc gccagcgtct gttgatgaag
    14701 ctgctttaac tgcttcgtca tattctgcat tgctccagcg accacggttg tatgaattac
    14761 ctgtgacaaa caaatcagta aagctgcttg ggtctgcata gtcagcagcc catccgccta
    14821 gtactgcgtc aaaatcaccc gaagttgagc ggtcaagacg tacagagaat ggaacaggtg
    14881 ttggtttgac tgttacgcca tccaagtttt cttggattga gttttgaaca tattcgatta
    14941 cttttttcga tgaatcatca tcagaagcca tcaaatcaaa ttctaaagaa tcaacgccca
    15001 attctttttt cgctttttcc caatattctt ttgctttatc tttgtcataa gaaactaatt
    15061 ttccagcttc ttttgcaaag tcttcgttcg ttgttggact ttttaccata tctgatggga
    15121 tcaatcctgt agagacaact gatccgtcac ctagtacttg ttttactaaa gcttcacgat
    15181 cgatagaata agaaatagct tttcttaggt tcacattgtt gaatggtgaa ttcttttcac
    15241 gttgattgaa ttcgatatag cttgtccgcg cttctttaac agaagtataa gctggatcgt
    15301 ttgcgttttg ctgtgctaac tcaccagaca agatcacgtc atctgcttgg ccatctttga
    15361 ataagttcaa tgcagtagaa gattctttta ctacactgac tttgatttca gaaagtttta
    15421 ctgtatcttt gtcccagtaa tgatcatttt tcttataagt ccattctgta tctgttcctg
    15481 gtccatcaaa atcttctaat gtgaatggtc cattataaac agctttatca cttgcagatg
    15541 cataggcatc cccattttct tccactacgg attgtttttg tgggaaaaat gatgggaaag
    15601 caagcagata ttggaaatat ggtgtttgtt tcgtcaattt gatttctaat tcatagtcac
    15661 caactgcttt gatccctagt tctgatttat ctttctttcc agctgtaatg tcttctgcat
    15721 tttcaactgg cgcatacagg taagcatatt cagaagctgt agctgggtct actgttcgtt
    15781 gccagccata aacgtaatcc gctgctgtaa caggatcacc atttgaccat tttgcgtctt
    15841 ctcgtaattt gattttgtaa gttaatccgt cttcactgac ttctgccaac tcgctagctc
    15901 ctgccggctg tggtttgctg tcttcatcaa gacggtaaat tccttcataa acgttattca
    15961 atgctgtaaa actgattgta tctgttgcaa ctgataagtc agctgtaggc atttcttgag
    16021 gcacgactaa gttaaatacg cctcctccat ctgctgaacc gctttttgct gaactactgc
    16081 ttgtggctga actgccgcca taacatccag caagtacaag gccgcatacc gcaaccaaac
    16141 caaatgtaaa tttctttt
//
LOCUS       DAHUWM010000052        15783 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHUWM010000052 DAHUWM010000000
VERSION     DAHUWM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.052"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(27..725)
                     /locus_tag="NK788_001142"
     CDS             complement(27..725)
                     /locus_tag="NK788_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCH6769484.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="NK788_001143"
     CDS             complement(718..2070)
                     /locus_tag="NK788_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCH6769485.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="NK788_001144"
     CDS             complement(2293..3693)
                     /locus_tag="NK788_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCH6769486.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="NK788_001145"
     CDS             3854..4591
                     /locus_tag="NK788_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCH6769487.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="NK788_001146"
     CDS             4716..5195
                     /locus_tag="NK788_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCH6769488.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="NK788_001147"
     CDS             5192..5959
                     /locus_tag="NK788_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769489.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="NK788_001148"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="NK788_001148"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCH6769490.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="NK788_001149"
     CDS             complement(6862..7464)
                     /locus_tag="NK788_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6769491.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="NK788_001150"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="NK788_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="NK788_001151"
     CDS             8145..8729
                     /locus_tag="NK788_001151"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCH6769492.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="NK788_001152"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="NK788_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCH6769493.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="NK788_001153"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="NK788_001153"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCH6769494.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="NK788_001154"
     CDS             10630..10809
                     /locus_tag="NK788_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769495.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="NK788_001155"
     CDS             10831..11856
                     /locus_tag="NK788_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCH6769496.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="NK788_001156"
     CDS             12031..13275
                     /locus_tag="NK788_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCH6769497.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="NK788_001157"
     CDS             13428..14375
                     /locus_tag="NK788_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6769498.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="NK788_001158"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="NK788_001158"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCH6769499.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="NK788_001159"
     CDS             complement(15277..15519)
                     /locus_tag="NK788_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769500.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="NK788_001160"
     tRNA            15640..15712
                     /locus_tag="NK788_001160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
BASE COUNT         5361 a         2828 c         3201 g         4393 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aat
//
LOCUS       DAHUWM010000053        15697 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHUWM010000053 DAHUWM010000000
VERSION     DAHUWM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.053"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(12..1535)
                     /locus_tag="NK788_001161"
     CDS             complement(12..1535)
                     /locus_tag="NK788_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCH6769501.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="NK788_001162"
     CDS             1787..2968
                     /locus_tag="NK788_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6769502.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="NK788_001163"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="NK788_001163"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCH6769503.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="NK788_001164"
     CDS             complement(5053..7764)
                     /locus_tag="NK788_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769504.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="NK788_001165"
     CDS             complement(7774..8475)
                     /locus_tag="NK788_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769505.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="NK788_001166"
     CDS             8661..9260
                     /locus_tag="NK788_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6769506.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="NK788_001167"
     CDS             9341..10354
                     /locus_tag="NK788_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCH6769507.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="NK788_001168"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="NK788_001168"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCH6769508.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="NK788_001169"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="NK788_001169"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCH6769509.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="NK788_001170"
     CDS             11802..13175
                     /locus_tag="NK788_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6769510.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="NK788_001171"
     CDS             13532..15676
                     /locus_tag="NK788_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCH6769511.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         4618 a         3133 c         2917 g         5029 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgt
//
LOCUS       DAHUWM010000054        15503 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHUWM010000054 DAHUWM010000000
VERSION     DAHUWM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.054"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            138..1475
                     /locus_tag="NK788_001172"
     CDS             138..1475
                     /locus_tag="NK788_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCH6769512.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="NK788_001173"
     CDS             1523..3652
                     /locus_tag="NK788_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCH6769513.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="NK788_001174"
     CDS             3627..4316
                     /locus_tag="NK788_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769514.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="NK788_001175"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="NK788_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="NK788_001176"
     CDS             complement(5546..5740)
                     /locus_tag="NK788_001176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769515.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="NK788_001177"
     CDS             complement(5790..6230)
                     /locus_tag="NK788_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCH6769516.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="NK788_001178"
     CDS             complement(6234..7079)
                     /locus_tag="NK788_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCH6769517.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="NK788_001179"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="NK788_001179"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCH6769518.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="NK788_001180"
     CDS             complement(8703..9650)
                     /locus_tag="NK788_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6769519.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="NK788_001181"
     CDS             complement(9873..10223)
                     /locus_tag="NK788_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCH6769520.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="NK788_001182"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="NK788_001182"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCH6769521.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="NK788_001183"
     CDS             11646..11966
                     /locus_tag="NK788_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCH6769522.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="NK788_001184"
     CDS             11988..12452
                     /locus_tag="NK788_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6769523.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="NK788_001185"
     CDS             12657..13262
                     /locus_tag="NK788_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCH6769524.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="NK788_001186"
     CDS             complement(13301..14590)
                     /locus_tag="NK788_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCH6769525.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="NK788_001187"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="NK788_001187"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCH6769526.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4838 a         2899 c         3180 g         4586 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tat
//
LOCUS       DAHUWM010000055        15184 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHUWM010000055 DAHUWM010000000
VERSION     DAHUWM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.055"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="NK788_001188"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="NK788_001188"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCH6769527.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="NK788_001189"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="NK788_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCH6769528.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="NK788_001190"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="NK788_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCH6769529.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="NK788_001191"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="NK788_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCH6769530.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="NK788_001192"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="NK788_001192"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCH6769531.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="NK788_001193"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="NK788_001193"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCH6769532.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="NK788_001194"
     CDS             complement(8053..8523)
                     /locus_tag="NK788_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCH6769533.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="NK788_001195"
     CDS             complement(8569..8994)
                     /locus_tag="NK788_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCH6769534.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="NK788_001196"
     CDS             complement(9061..9330)
                     /locus_tag="NK788_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCH6769535.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="NK788_001197"
     CDS             complement(9341..10936)
                     /locus_tag="NK788_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6769536.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="NK788_001198"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="NK788_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCH6769537.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="NK788_001199"
     CDS             complement(14489..15049)
                     /locus_tag="NK788_001199"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCH6769538.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3926 a         3439 c         2692 g         5127 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaa
//
LOCUS       DAHUWM010000056        15082 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHUWM010000056 DAHUWM010000000
VERSION     DAHUWM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.056"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="NK788_001200"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="NK788_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCH6769539.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="NK788_001201"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="NK788_001201"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCH6769540.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1945..2643
                     /locus_tag="NK788_001202"
     CDS             1945..2643
                     /locus_tag="NK788_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCH6769541.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2811..5450)
                     /locus_tag="NK788_001203"
     CDS             complement(2811..5450)
                     /locus_tag="NK788_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6769542.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6069..7100)
                     /gene="queA"
                     /locus_tag="NK788_001204"
     CDS             complement(6069..7100)
                     /gene="queA"
                     /locus_tag="NK788_001204"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCH6769543.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7293..8468
                     /locus_tag="NK788_001205"
     CDS             7293..8468
                     /locus_tag="NK788_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6769544.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8472..9113
                     /locus_tag="NK788_001206"
     CDS             8472..9113
                     /locus_tag="NK788_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769545.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9110..10027
                     /locus_tag="NK788_001207"
     CDS             9110..10027
                     /locus_tag="NK788_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6769546.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10024..10695
                     /locus_tag="NK788_001208"
     CDS             10024..10695
                     /locus_tag="NK788_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769547.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11236..11724)
                     /locus_tag="NK788_001209"
     CDS             complement(11236..11724)
                     /locus_tag="NK788_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCH6769548.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11925..12191)
                     /locus_tag="NK788_001210"
     CDS             complement(11925..12191)
                     /locus_tag="NK788_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCH6769549.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12191..12454)
                     /locus_tag="NK788_001211"
     CDS             complement(12191..12454)
                     /locus_tag="NK788_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCH6769550.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12930..13115
                     /locus_tag="NK788_001212"
     CDS             12930..13115
                     /locus_tag="NK788_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6769551.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13257..15080)
                     /locus_tag="NK788_001213"
     CDS             complement(13257..15080)
                     /locus_tag="NK788_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCH6769552.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4368 a         3233 c         2850 g         4631 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
     1921 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
     1981 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
     2041 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
     2101 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
     2161 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
     2221 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
     2281 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
     2341 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
     2401 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
     2461 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
     2521 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
     2581 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
     2641 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
     2701 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
     2761 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
     2821 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
     2881 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
     2941 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
     3001 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
     3061 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
     3121 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
     3181 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
     3241 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
     3301 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
     3361 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
     3421 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
     3481 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
     3541 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
     3601 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
     3661 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
     3721 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
     3781 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
     3841 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
     3901 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
     3961 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
     4021 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
     4081 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
     4141 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
     4201 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
     4261 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
     4321 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
     4381 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
     4441 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
     4501 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
     4561 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
     4621 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
     4681 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
     4741 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
     4801 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
     4861 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
     4921 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
     4981 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
     5041 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
     5101 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
     5161 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
     5221 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
     5281 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
     5341 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
     5401 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
     5461 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
     5521 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
     5581 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
     5641 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
     5701 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
     5761 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
     5821 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
     5881 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
     5941 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
     6001 gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt
     6061 tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca
     6121 atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg
     6181 actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca
     6241 tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat
     6301 ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg
     6361 ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca
     6421 ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct
     6481 acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct
     6541 ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca
     6601 gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct
     6661 cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg
     6721 ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt
     6781 gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta
     6841 ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga
     6901 gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg
     6961 atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga
     7021 gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca
     7081 aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc
     7141 atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa
     7201 ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt aatagatagt
     7261 tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa
     7321 tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt
     7381 tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc
     7441 gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg
     7501 atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc
     7561 acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag
     7621 aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag
     7681 atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc
     7741 ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca
     7801 ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg
     7861 tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga
     7921 gtgaaggccg tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg
     7981 atttcgtaga agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa
     8041 gagtcggcga agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag
     8101 aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga
     8161 atgtattaaa aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca
     8221 gtgtcggcga cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg
     8281 ataccttgca acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga
     8341 acaaagtcgt aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt
     8401 ggggagaaga tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg
     8461 aggtctgacc tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt
     8521 ttgaacatct ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc
     8581 ttggtgtctt actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg
     8641 ccttgcagac aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag
     8701 gtaaattacc tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata
     8761 cgtacattgg gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga
     8821 tgactgagtt ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg
     8881 ctggtattcg tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg
     8941 gcgctggtgg tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca
     9001 ttttggcagg tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc
     9061 tcttggaaca ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat
     9121 actaaaaatg accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg
     9181 tcttgccagc aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc
     9241 acagatctta gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc
     9301 aatcatcaac aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc
     9361 ttcaatttca gcggctcgct acacaggaac agatttaacg actacattga accttcctcc
     9421 tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg
     9481 tcaaacgtgg ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga
     9541 caccgcacaa aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt
     9601 agtagctggt gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca
     9661 agaaacttat ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga
     9721 cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc
     9781 cagttatgat ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc
     9841 tccagttgta gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa
     9901 gttaggtcat accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa
     9961 tctagtggaa ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg
    10021 gaagtgaagc aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa
    10081 aatggctcct atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc
    10141 ctctttgcag cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta
    10201 gcaaactggg tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc
    10261 tccattttaa tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg
    10321 tattccctct taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac
    10381 atccttgatg tcggcaaagg catggggatg actgctagac aacgactatt catggtagag
    10441 ttaccactct ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc
    10501 gggataaccg cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga
    10561 ggaaccaatg caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta
    10621 atggcgatca tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc
    10681 cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc
    10741 aaaggctttt ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt
    10801 atgggtaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
    10861 gccaacgggt tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt
    10921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
    10981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
    11041 agccttttcc atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga
    11101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
    11161 tggctaaaaa catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
    11221 accggtacac gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc
    11281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
    11341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
    11401 cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
    11461 tttggatacc cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt
    11521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
    11581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
    11641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
    11701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
    11761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
    11821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
    11881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
    11941 caggcagaat aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
    12001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac
    12061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
    12121 aaattcgttt gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta
    12181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt
    12241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
    12301 gaatcgctct ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa
    12361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
    12421 gagcagtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
    12481 ttatagcgta cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa
    12541 cttttcctct tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc
    12601 tcacttctta ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta
    12661 tcatctttag caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg
    12721 aaggttcctc gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga
    12781 aagatttgta acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa
    12841 atgattgcct ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga
    12901 ccgttgggtt tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt
    12961 cacttgacga ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa
    13021 aggaagaagc tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg
    13081 atcaagacat caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata
    13141 cgctgttccc atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca
    13201 gaccctctag cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat
    13261 ttcttcaaat ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat
    13321 cttaaccgca aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat
    13381 tgaggaatca acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct
    13441 acataacgga taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg
    13501 acatctggga aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg
    13561 tctagcgaaa catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca
    13621 ccgatattta cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca
    13681 tgtagttttg catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg
    13741 atcttataaa aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct
    13801 aaccgataga caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg
    13861 gcaatcgatt tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt
    13921 tgagataaag caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt
    13981 gagccatgga aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg
    14041 gaatagccta gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc
    14101 agattgtatg caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc
    14161 agcgctgtag caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca
    14221 atttgagaca aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat
    14281 gtgatcccca cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca
    14341 ttttttgctc ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt
    14401 aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca
    14461 gccgctcctg gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa
    14521 ccaacaacga tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac
    14581 gatgcagatt ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca
    14641 gaaatagcga cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct
    14701 gctgagacag atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc
    14761 aagccagcat tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga
    14821 taagcatgga tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa
    14881 gcggcaatgg gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg
    14941 atttgttccg taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca
    15001 gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac
    15061 aaccttttta agtaatccaa tt
//
LOCUS       DAHUWM010000057        14981 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHUWM010000057 DAHUWM010000000
VERSION     DAHUWM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.057"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..774
                     /locus_tag="NK788_001214"
     CDS             <1..774
                     /locus_tag="NK788_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6769553.1"
                     /translation="DNCTANTVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQF
                     VSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINH
                     VIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILE
                     DNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSI
                     QEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1348..1917
                     /locus_tag="NK788_001215"
     CDS             1348..1917
                     /locus_tag="NK788_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCH6769554.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1904..2764
                     /locus_tag="NK788_001216"
     CDS             1904..2764
                     /locus_tag="NK788_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6769555.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2954..3658
                     /locus_tag="NK788_001217"
     CDS             2954..3658
                     /locus_tag="NK788_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6769556.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3663..5498
                     /gene="walK"
                     /locus_tag="NK788_001218"
     CDS             3663..5498
                     /gene="walK"
                     /locus_tag="NK788_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCH6769557.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5495..6811
                     /locus_tag="NK788_001219"
     CDS             5495..6811
                     /locus_tag="NK788_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769558.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6812..7681
                     /locus_tag="NK788_001220"
     CDS             6812..7681
                     /locus_tag="NK788_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCH6769559.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7736..8545
                     /locus_tag="NK788_001221"
     CDS             7736..8545
                     /locus_tag="NK788_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCH6769560.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8648..9277)
                     /locus_tag="NK788_001222"
     CDS             complement(8648..9277)
                     /locus_tag="NK788_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCH6769561.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9447..10739
                     /locus_tag="NK788_001223"
     CDS             9447..10739
                     /locus_tag="NK788_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6769562.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10815..11477)
                     /locus_tag="NK788_001224"
     CDS             complement(10815..11477)
                     /locus_tag="NK788_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCH6769563.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11702..11986
                     /gene="groES"
                     /locus_tag="NK788_001225"
     CDS             11702..11986
                     /gene="groES"
                     /locus_tag="NK788_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCH6769564.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12037..13662
                     /gene="groL"
                     /locus_tag="NK788_001226"
     CDS             12037..13662
                     /gene="groL"
                     /locus_tag="NK788_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCH6769565.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13751..14899)
                     /locus_tag="NK788_001227"
     CDS             complement(13751..14899)
                     /locus_tag="NK788_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6769566.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
BASE COUNT         4945 a         2549 c         3142 g         4345 t
ORIGIN      
        1 gataactgta cagcaaatac ggttattcat tatcacgcat ttatacgcaa agctcttcaa
       61 gaggctgtta ttacagaatt gataagtaca aatgtagcag ataaagtaag aaagccaaaa
      121 aagaaacaat ttgtttctca agtttataat caacaagaga tcttgaaatt acttgacatt
      181 ttgtcagaag aaaaactcta tttggtagtt cttttaacag cattttatgg attgcgaaga
      241 agtgaagttt taggattaaa atggtctgct attgattttt taaacaaaaa aatcatgata
      301 aaccatgtaa tcatagaaaa tccagagaat atgtcacagt taataaagaa agatcaaaca
      361 aagaactctt ccagctatcg tacattacct ttggttgaaa cgattgaacg agcattagta
      421 aatcaagcta aatggcaaca agataatcgt attcttttag gagatgatta tattttagaa
      481 gataatgagt acgtatttac tatggaagat ggacggttga tgaagcctca atatgtaacg
      541 catcgtttga gtaagttgat taagaaaaat ggattgaaaa aaattcgctt tcatgacttg
      601 cgtcattcta atgcttcaat aatgttagat agtggccaga atatgaaatc cattcaagaa
      661 tggctgggac atgcatcgta ttcgacaact gctaatttat acacgcattt aaccgcagga
      721 gtaaaagatc gtgcagcaga agcattagaa aacgcatttg ggtttaaaga ataaaaaaac
      781 aaaaaacaat atctttgggg tatcgcttct tgtttttaga atttttttgt tggcaatttg
      841 ttggcagtat gttggcaatg aaaaaattca caaactaaaa acctatttat aaagctttat
      901 aaaacttttt tcacaaatag agcatttggt taagagtcag ctgctctacc aactgagcta
      961 acggcccgta caaaaagtac ttaataatag tagcactaag aattttaaaa tgcaagacta
     1021 catttaaaga aaatcagaaa aatctttact tccttatagg aaatcattca tagattattc
     1081 acatttgctc gctattatat aagcatacca acaaacaaag ttcttgcaag agaacaaacc
     1141 ctaacacttt tttcattttt tactcctttc ccgccgtctc cccggcgggt ctcctgttct
     1201 taacgataaa agacaagaag gcttagcctt cttgtttttt gttttcatgg aatcccgcta
     1261 atcaaatcac ctagttaaag caactagatt gaatgtttat cataagtctg atatcatgtt
     1321 tatgaaatga aaaaaggagc ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg
     1381 gggaaggaac tagaaacatt ccgtctgcct cactgggaag aactccctga cttagaactt
     1441 tatatggacc aagtcagaac gttagttgat cgttatcttt cacctgtgat acaaggagaa
     1501 aagcatccat tgcttacctc ttctatggta aacaattacg ttaaactagg tttgatacca
     1561 gcaccagtta aaaaaagata taacaaagaa cacgtagcat ttttacttgc aatcactacg
     1621 ttgaaacaag tattgactat tcctgaaatc aaagaaggga tattgttcca aggaaaaaca
     1681 gtaggaattc gtgaagccta taatctattt tgcgatgaac aagaagcagc tgtgtggatg
     1741 gtcagccaat tagcacaagg taaatctcat ccgcaaaaat tcgaccaggc aacccctgta
     1801 gaatatattg ctgtaagagc ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa
     1861 acgattgttc tagaaaccga atatttaaaa gaggagaaaa caaatgaaaa acaataaaat
     1921 cgcaatttta gtagattcag gtacggatgt tccagaagaa ttgataaaaa aatacgagat
     1981 gtatatgatc ccattgaaaa ttatttataa agatcaggta tatacagata aaattgacat
     2041 tacccctgaa gagatctatc aacgtttgcc agaggaaatc cctggtacat ctttgccgga
     2101 tggtgagtcg atcacagaaa tctttgagca aatcaaaaaa gacggttatg aaaaggtact
     2161 tgcggtaaca atttccagtg gattgagcgg gacatataat gtggttcgtt taattgcaca
     2221 acagcaagaa gaattggaag catatgtact agatacgaaa aatattggta ttggtgcagg
     2281 tttttctgcg atccaagcag caaaatggct ggaagaagga gtagaatggg atcaactgat
     2341 cagtaatctg aatgagttag tcgaaagaac aaaagttttc tttaatgttg cgacattgga
     2401 atatcttcaa aaaggtggac ggatcggttt agttgcttct attgtaggaa ccgctttaaa
     2461 gcttaaccca atcatctcat gtaatgaaga agggatttat tacacagtag gaaaagctag
     2521 gggacgtaaa aaaagtttgg ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc
     2581 aaaggtcttc aacttagctg ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga
     2641 agcgagactt aaaagagagt tcccacaagc aaaccaaatc tactttggcc agatttctcc
     2701 ggcattagta gtccacactg gtccgggatt attgggtgtc ggtgtacaag ttttagaagg
     2761 ataaatcaaa aaaaggaaac actgaggtga aaatcagtgt tttccttttt tattgaagtt
     2821 agaacaaaaa ataataaagt attttgcact ttcattttaa ttttctcttt tgttttatag
     2881 gcaaagatga aacaaaagca gaaatacggt ataatgagaa ggtatgacaa taatatataa
     2941 gaggtggctc tttatgaaaa aagttttagt tgtcgatgat gagaagccga tttcggatat
     3001 cgtcaaattc aatttagcca aagaaggcta tgacgtttat actgcatatg atggggaaga
     3061 agccttggaa aaagttgctg aggtagaacc tgatttgatt ttacttgatt tgatgctacc
     3121 taaaatggac ggcttagaag ttgctcgtga ggtacgcaaa acttatgata tgccgatcat
     3181 tatggtaaca gccaaagatt ctgaaattga taaagtccta ggcttagagt taggcgccga
     3241 tgattacgtg accaagccat tttccaaccg agaattagtt gcacgtgtca aagcaaatct
     3301 tcgtcgcggt gccacggctg cgaaggaacc agaagaggca gcacctgcgg aattgacgat
     3361 tggtgatttg acgatccatc cagaagctta catggtgacg aaacgaggag aaacaatcga
     3421 gttaacacat cgtgaattcg aactactctt ttacttggcg aagcaccttg gacaagtgat
     3481 gacaagagaa catttattac aaacggtttg gggttatgat tattttggag atgtacgaac
     3541 agtggatgta actgtacgtc gtttgcgtga aaagatcgaa gataatccaa gccatccaaa
     3601 ttatttagtg acacgtcgtg gtgtcggtta ctatttgcgt aacccagaac aggagtaatt
     3661 ggatgaaaag aaaagtccgc ttttttcgat cagtcaattt taagatcgct attaccttta
     3721 ttctgatttt attgatctcg atcgaaatta ttggtgccta ctttatacgt ggtttagaac
     3781 gttccacgat caatacattt attaaagata tgaatcaaac ggtggaatca ttagcaacaa
     3841 caatcagccc ggaattgaat cgaaaagata atgcagatga tgaagaggta aatgcgaata
     3901 tcaaaagatt tattgaaaac agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg
     3961 aaaaagggat catccgagga acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa
     4021 atgattatgt tgatatcaat gatttcacga ctaaacgtta tgttgcttta gataacgacg
     4081 ataaacgggt gaatatcaat gttcaaccga tcctgtcccc gaccggcgat gccgttatcg
     4141 gggctcttta cgtcaaaagt aatatcgaac aaaaatattc ggaaatcaat aacacagcag
     4201 tcattttctt tactgcctca ctaattgcgg catttatttc aatgatcgta tctgttttag
     4261 ttgctcgttc gattacacag ccgatcggtg aaatgagaga acaagccatt cgaatcgcta
     4321 gaggagacta cagtcggaaa gtcaaagtgc atggacaaga tgaacttgga cagctggctg
     4381 ataccttcaa tcagttagct gaacgtattg aagaagctca agatacaatg gaagctgaac
     4441 gcaatcgttt ggatagcgtg ctatcccata tgacagacgg cgtcattgcg acagatcgca
     4501 ggggtaaagt cattacgatc aatgatatgg ctgtttcctt gctagatatc aaaaacgagg
     4561 aagcaatcgg acaatcgatt ttgacacttt tagatatcga agaagaatat acgttgcgga
     4621 aattattaga agatccaaac gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg
     4681 atcagatgat cttgcgagta gacttttcta tgatccgtcg cgaatcaggt tttatcagcg
     4741 gattagtagc tgtcttgcat gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg
     4801 aattcgtctc taatgtttcc catgaacttc gtacaccgtt gacaagtatg cgtagttata
     4861 tcgaagcatt gagcgaaggt gcgtggaaag acgaggaagt agcaccaaat tttttgaagg
     4921 taacgctaga tgaaacagac cgaatgattc gaatgatcaa tgacctctta aatctatctc
     4981 gcatggatac aggtaatacc cagcttcaat tagaatatgt caatttcaac gagttagtga
     5041 attttgttct tgaccggttt gatatgatgg tgggaaatca ggaaaaaaat tataagatcc
     5101 gtcgtgaatt tacgcaaaga gatctttggg tcgaagtaga tacagataaa atcatccaag
     5161 tagtcgataa tatcatgaat aacgcgatca agtattcacc agatggggga acgattactt
     5221 gtcgcttatt agaaacgcat aataacgtga ttttaagtat caccgatcaa ggattgggga
     5281 ttcctaaaaa agatctgaac cgagtgttcg agcggttcta tcgagtagat aaagcacgtg
     5341 caagagcaca aggtgggaca ggacttggat tagctatttc tagagaagtg attaaggcgc
     5401 atcgaggcgc tatttgggca gagagcaagg aaggaaaagg gtcgactttt tatatctcgc
     5461 tgccatatga accttatgag gaggaatggt gggaatgaga atctctgaaa aagtcgttcg
     5521 tataggactc attttgatgg ttgcgttaag catatacttc tcttatgcaa tctggctgag
     5581 cccagcagga aaaagttcaa tcaacgtaga tgagacaaac actcaaatga tcgagtctca
     5641 aagcaatcga aaaacttccg aagtctttct tccgcttcat gttacatggc accattcggg
     5701 aatgatccaa gaaaccaaca gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa
     5761 agcaagattt ggaaagcttt ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt
     5821 gaaaaatctg aactctggtt ttgagttggc ctataatgcg ccttttttgc taagagaata
     5881 caaggaagca tttgatttag atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat
     5941 gtattttact agaatacaat tcgatcaagg aaataaaaaa atccgatttt tgaattattc
     6001 gaaccatctc gtatatgaag caaatatttc gttgaattgg acagatatca ataaagaatt
     6061 aacagcaaaa gacacaaaat ggacagaaat gacaacagaa ccatcgacga tcgacacgca
     6121 atatgctacg aaacattcga tcaaactgaa gaagtatagt tatatcctgt cacagcaacc
     6181 ctatacactt tttagaaatg cttttttcca gaaaccagat agcgtgaaaa ataatgatga
     6241 aagcaatgaa ttgatttttt acggtgggaa tgaaacaatg tctatccatt cggaaacgca
     6301 agtagttgat tttcgtggag agctgccaga gaaaaaagaa aatgctagtg tattttctga
     6361 aagtttcccc tatattagca aattaggcag ttctttaggg aacctcagat attttgaccg
     6421 cagtgacaat acgatcgatt atcggatatt cgtggaaggc ttcccggttt ttgggacaga
     6481 cagcagagga cagattggtg tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa
     6541 tatccaaacc agtttgaata cgattcaagt gccgatacct tcagacgaag aagtagaact
     6601 tgcaagtacg aatgaaatca tccagcagtt aaaagctaag ggagcagata gcaacaagat
     6661 tggttcagtg atcgttggct atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga
     6721 tctcttacct gaatggtatg tgaaatataa agataactgg tattcagcta acgaccttct
     6781 ggaaaaatta cctggattgg aggcgaatta attggatttc aaaaaaatcg aatggatatt
     6841 tttcgtcgct tttttagggt tgaatgtgtt tttattcagc agttatcatg acgcggtata
     6901 ccaagaaaac aatgtcattc gctctaatca gactgaatca atccaacaac gtctggctag
     6961 cgacgatatc acttacacgg ggaagatttc gtctgagaac aagcaaggct attatcttag
     7021 cgcggagcag accaatctta gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg
     7081 cttgctggca agtggtgtgt ttttagacgg tgatgttttg acgaaatctt tatccagttc
     7141 agatgctgaa aagaacgtga tcgatccaga taaaattagt agttccttgg aagatttttt
     7201 atctaacaaa gacaatattt tattcggtaa tgattatgtg tacacgaaaa acttttctca
     7261 tttagaagaa gaaccactag aaatccaagc aagccaagag tatgaaggca tcccattcaa
     7321 tgatgataca gcaaaaatcg ttctttcttt agaaaaaaac gataatgatt atagcatcac
     7381 gaaatacatg cagacgcatt tatctgatat cgagcagctg cgcgaaaaga cggaactgta
     7441 tacggaagag gaagcaatca atacattgta catcaataac aaaataccaa gaggatcaaa
     7501 aattttatgg cgtcagctag cttattcctg tattctgaaa gtccgggaaa aaaatgtgta
     7561 tgttcctgtt tggcatgtag cgatcgaaac aagcgataaa gtggtccaaa ttgagagcgt
     7621 caacgctttc agcaatacga tcgtgacaaa caatactgta ccaaaagtgg aagatcaata
     7681 aaaaagcgag tataatgaaa aaagtttaca aagtaagaag ggaagtagga gtcaaatggc
     7741 tgaagaaaaa ccagctttta aaatcagtat cctagcaagt ggaagttcgg gtaattcact
     7801 ttatatcgaa agtgaaaaaa aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat
     7861 cacttccttg atggctcaaa ttgatcggac accggaccaa ttagatggaa tacttgtgac
     7921 gcatgaacat cgagatcata tacatggcgt gggtgtcttg gctcgtaagt atcatttgga
     7981 tgtttttgcc aatcagcaga cttgggaagc gatggaacca ctgattggaa atgtaccagt
     8041 ggaacaaaaa catctatttg agatggggaa agtcagaaca ttcggcgata tcgatattga
     8101 gagtttcggc gtctcccatg acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa
     8161 tcggtcattc gtcatgttga cggacaccgg atattgcagt gattatatgc gaggtgtcat
     8221 tgaaaatgcc gatggttatt tgatggaaag taatcatgat cttgagatgt tacgcatggg
     8281 accttatccg tggaatttaa aacagcgtat tttaggagat agaggacatc tttccaacga
     8341 agacggtgca cttgtcatga ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg
     8401 ccatcttagc aaggaaaaca atatgaaaga acttgctcat ttaacaatgg aaaatgtttt
     8461 gaaagaaaaa gattttggcg ttggacatga ttttgaaatc tatgatacag atccagatga
     8521 agcaactgag ctattttcga tttaaacagt tcaatcaata aaaatataaa aagcagaaga
     8581 taaaatcaat cagggttggt tttatcttct atttttattt ttttagcata ttaaaaaaat
     8641 aaccgtatta tcgactgact acactgatgt gctgcttttc atgtttctca tccagtatct
     8701 catctactaa tgcaatcgcg taatctgcca tgctgatcgt actgtcgcta gcagcgtttc
     8761 tttgtaaata atcatcactt aacaaataag tacctgtacg tttgccatgt ggatcaaata
     8821 aagaagctgg actgatataa gtccaatgta gatttttaga ttgtttcaat tttaacaatc
     8881 cttcgtacat attgaaagct gttggataga agggtgcatt tttaggtact tggtcaatca
     8941 tacgagtcgt cttttctgta tctaagaata acgaagaggc acccccaacg acaattaagc
     9001 gtgtatcggt tccttcaagc atctcaatca aatgggacaa agaagtttga tgcatttctt
     9061 cttttccttt gggagcgttg aaagcatcaa cgacaacatc aaaagatgca agatcagtag
     9121 ttgtcaactc aaataaatcc ttttgtataa acggaacatc tgatggcaat ttttctggat
     9181 gacgcacgat tgccgtcacg tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac
     9241 ctgcatggcc agtagctcca atgagtgcaa ttttcataaa aaacctcctt ttttattcct
     9301 gtccattgta agcagttgct gaatattcgt caaagaatcc gatttttgaa aagctcagtg
     9361 cgcattcctt tcatggttcg tgatcattgg gtatagttaa attaagaaaa ctgattgata
     9421 aaaatagaag ttgaaggaga taagaaatga aagagtggtt agatcgtgcc tttttaccaa
     9481 tgataacatc tgttgtgaat cttagaccct taactgcttt gcgtaatgga atgacttaca
     9541 cgatcccaat aattattgtt ggctcgctgt ttttcttatt tgctcatttt cctgtggatt
     9601 acatcgctca gtggatagac aaaacaggat tttctatgta ttttacacaa gtataccagg
     9661 ctacatttga tgtgatagcc ttttggatcg tttttgccat tgcatacgct tatgcaaaag
     9721 aagaagacat cgatcgctta ccagtcggca taacagcctt gtctgcttat ctactgctta
     9781 ttcagccact aaatactcag atagatactg tctggacagg tgctgaaggg atactaggag
     9841 cagttacaac tgggctcatc atcggatggg gctatgcacg tttagtaaga cttgcgaaag
     9901 ggtggaagca aagagagaag atacctgaaa atgtttggca gtcttttact tcattgattc
     9961 caatcattgt tatagtcact tttacgctta tcggttcagc ggtttttcaa catttgttca
    10021 gaatgactgt tgttcaagga gtatggacat atgtccagct tcccattcaa tcgttcatag
    10081 acacgttgtt tggtgtgctt ttattaggtt tttttgttcc gtttttatgg ttatttggtc
    10141 ttcatggatc tgcgatggtc aatgggttgg ttagtcccgt cttgcaagcg aattcattag
    10201 caaatgcaga aatcttagcc tcaggacaag agttgacggt ggcaaatggc ggacatatcg
    10261 ttactcagca atttctcgat cagttcatga cggttacggg tgctggattg acgttagggg
    10321 ctgtattttt tatgacggtt tttgcgaaat cgaagaaata cagagaatta ggaaatcttg
    10381 ctcttttacc agcctttttc aacatcaatg aatcaattat tttttccaca ccgatcgtga
    10441 tgaacccgat gatggctgtt ccttttattt tcgcaccggt cgtttcagga ttgattacct
    10501 attccgcttt atattttgga ttcgttcctt tatttacagc agtccaagtc ccttggacaa
    10561 caccgccgat cctctcgggt ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta
    10621 tcgtgttgtc aattagtttt ttcatttatt tcccattttt gaaaaaaata gattctgcaa
    10681 actgcaaaaa ggaaagacag acaaaaaatg atggagcagc agaaaagatg aacgactaaa
    10741 aaacaaaaat actcctgtcg ctttagattg ggaaaatcac caatctagga tgacaggagt
    10801 atttatagtc atgtttatac tagccctttt tggacagcta attgaacaag aacaactaat
    10861 gtgttcattc ctacgtgtgt gatcatcgat gtccagatcc gaccagttgc tttgtactct
    10921 aggctgaaga agaaacctag gaaaaagtag attagcagat gaccatcatt atgagcaaac
    10981 gcaaacagca aagagctgat cactgctccg acccaaaaat ttgaatatcg actgatgatc
    11041 cccagtatac tgcggcggaa tacgaactct tccatgatgg gtccagcaac agttgtcgcc
    11101 aaaataaaag cgggtgcttc catgatcatc tggatgatat tttgtgtatt ctcggaaggg
    11161 gttgctttgc caaacaatac agattctaac atgacagcga tgctttgcag aatcaatgcg
    11221 acaaagattc caaccaatcc ataaataaca atctttggag cagaagcttt ttttaccgct
    11281 gattcgatcg ctaagggttc acgttgctta aaataaaaaa tagctaggac aacggcgcct
    11341 agtatataca tgactgcagt tgctgtgatc aattcagacg tagattttac aagtcctatc
    11401 gaagctagga tcaagggtga gataaaaatt agaccatagc aaaaaatgct gaggaagcta
    11461 tattttttta aagacatgta attcctcctt ttaagggata acaagcatgc aaggcaagct
    11521 tatatcgatt gatttcaatt atatcataat ttcaataagc agctgtcaga ataaaatttc
    11581 ttttctttga tttaaaagct tgcaaaaaag agggagaatg agtattatta aaaatgtagg
    11641 ttagcactcg aaggttaaga gtgctaacag attaaaaaaa gagttatcgg agggatttat
    11701 cgtgttaaaa ccattaggtg atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac
    11761 tgtcggagga atcgttttag cttctgctgc taaagagaaa ccccaaactg ggacagttgt
    11821 tgcagtcggt gaaggccgtt tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc
    11881 aggcgaccaa gttatgttcg aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga
    11941 atatctgatc gtcgcaggaa aagatatcat ggcgattgtt gagtaaattt ggaagataaa
    12001 aaaataatca aaaataatca atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca
    12061 gaagacgcac gcgcagctat gttacgcggg gtagataaat tagcagatac agtgaaagta
    12121 acattaggac ctaaaggccg aaatgtcgta ctagaaaaat cttacggctc tccattgatc
    12181 acaaatgatg gtgtgacgat cgcaaaagaa atcgaattag aagatcattt tgaaaacatg
    12241 ggtgcaaaac ttgtatcaga agtagcatca aaaacaaatg atattgcagg agacggtaca
    12301 acgacagcaa ctgttctgac acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca
    12361 ggtgctaatc ctctaggcat tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa
    12421 gaattgcata atatctcaac tgttgttgat tcaaaagaag ccattgcaca agtagcagct
    12481 gtttcttcgg gttctgataa agttggacat ttgattgctg atgcaatgga aaaagttgga
    12541 aacgacggtg tcatcacaat cgaagaatca aaagggatcg aaacagaact agatgttgta
    12601 gaaggtatgc agtttgatcg tggttactta tcacaatata tggtaacaga taacgacaaa
    12661 atggaagctg ttctagaaaa cccatatatc ttgatcacag acaaaaaaat ctctaacatc
    12721 caagatattc tgccattgtt agaacaaatc ttgcaacaat cacgtccatt gttgattatt
    12781 gctgacgatg tagatggtga agcattgcca actcttgtat tgaacaaaat ccgtggaaca
    12841 ttcaacgtag tagctgtaaa agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa
    12901 gatatcgcta ttttgacagg tggtacagta attactgacg atctaggttt agaattgaaa
    12961 gatgcaacaa tcgaaaactt aggaaatgct tctaaagttg ttgtagataa agacaataca
    13021 acgatcgtag aaggttctgg agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa
    13081 aaccaaattg ctgaaacaac ttctgatttt gaccgcgaaa aattacaaga acgcttagct
    13141 aaattagcag gtggtgtagc tgttgttaaa gttggtgcag ctactgaaac agaattgaaa
    13201 gaattaaaat tacgcattga agatgcattg aatgctacac gtgccgcagt agaagaagga
    13261 atggtctcag gtggtggtac agctctagtg aacgttatca gcaaagtttc tgctgtagaa
    13321 gcagaaggag acgtggcaac aggtatcaag atcgttgttc gtgctttaga agaaccaatt
    13381 cgtcaaatcg ctgaaaacgc tggttatgaa ggatcagtga ttgttgataa attgaagaac
    13441 gtggaactag gcactggttt caatgctgct actggagaat gggtaaacat ggttgaagca
    13501 ggtatcgtag acccaactaa agtaacacgt tctgccttgc aaaatgccgc ttccgtttct
    13561 gccttgttat taacgacaga agcagtcgtt gcagataaac cagaaccagc agcgccagca
    13621 gctcctgcaa tggatccatc aatgatgggc ggtatgatgt agtaattaag aaagaaccct
    13681 attttaacag ggttctttct tctttttttg ttttatttta agcaaaaggg gcaaagaagg
    13741 ggcaaaaata tcaaaggcta tttagtttat caattacttg ttgtttggct ctttttgtta
    13801 catggttata aatagacaga gttgtgtttg catcagaatg ccctacacgc tccataatgg
    13861 ctttaagagg tacgcctaat tcagataata gtgaaacgtg gctatgtctg aatatatgtg
    13921 aggatagact tttttctaat tccaactctt cctctacttt atggagtata gcgttaaatg
    13981 agtgcagcgc aagcggagtg ccacttgtag atataaatat atattgatcg ggatctgtgg
    14041 gtctacctgc aagaatattg tcagctatca cactttcaat caattctttt gcacgattgg
    14101 gtaattgcac ttcacgttgc gaataagtat tttttggagt tgtttttata gcattatcca
    14161 ttttcacaga tgtgtaatct aaggtcccat taatggaaat tttcccatcc tcatagtcct
    14221 tcatttgcaa agctagcaat tccccatatc tcaaaccagt taaatataga aactcagcta
    14281 ttatgccgtg tagttttctg cgaggattgg agtatagctg tttcagtatt tgatcaattt
    14341 cttctttctc cagatatttt ttattcatag aaagccttct tttttcttct tccacttttt
    14401 taggatggat tttaactgct agcgcagggt ttctttgaat gtattttcta tcgattgcat
    14461 agtttagcat aacagataga gttgtttttg tttgttttgt gtagttcagt gagaggtcac
    14521 caaacgtata catatcttca attatcttat taatgagtgt ctcatcaatg tttctaacga
    14581 tcgtatcatc gcttatgtgc ttagaaacat gtttcatcat cattggaacc ttcaaatagc
    14641 tagtacgttt aacatgctgc ttataatatt cataccattc tttatacagc tcaccaaaag
    14701 tgatatctga tttattgtaa tcttcgagtg cttctttaat ttttttatct agaattttct
    14761 gagctttttt ccacgcttgt ggtgaattac ttgtaagtgt tgtagatttt tttcgtgttt
    14821 tttctgtata aggatctaca tatctttcaa taaacttgaa ttggccatct ttggtctgtt
    14881 caacccacac ttttaacatc tcctatcatt tgctataata ggcataacaa atagacctat
    14941 ataggtttgt tttctaaaag cacgctctta ctctggacgg t
//
LOCUS       DAHUWM010000058        14815 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHUWM010000058 DAHUWM010000000
VERSION     DAHUWM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.058"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(296..1024)
                     /locus_tag="NK788_001228"
     CDS             complement(296..1024)
                     /locus_tag="NK788_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCH6769567.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="NK788_001229"
     CDS             complement(1002..2576)
                     /locus_tag="NK788_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCH6769568.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="NK788_001230"
     CDS             2805..3743
                     /locus_tag="NK788_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769569.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="NK788_001231"
     CDS             3755..4666
                     /locus_tag="NK788_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769570.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="NK788_001232"
     CDS             4690..6162
                     /locus_tag="NK788_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6769571.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="NK788_001233"
     CDS             6213..6824
                     /locus_tag="NK788_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCH6769572.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="NK788_001234"
     CDS             6854..8767
                     /locus_tag="NK788_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCH6769573.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="NK788_001235"
     CDS             8895..11876
                     /locus_tag="NK788_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCH6769574.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="NK788_001236"
     CDS             11873..13534
                     /locus_tag="NK788_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCH6769575.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="NK788_001237"
     CDS             13574..14506
                     /locus_tag="NK788_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCH6769576.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAHUWM010000059        14763 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHUWM010000059 DAHUWM010000000
VERSION     DAHUWM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.059"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(54..827)
                     /locus_tag="NK788_001238"
     CDS             complement(54..827)
                     /locus_tag="NK788_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6769577.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            971..1321
                     /locus_tag="NK788_001239"
     CDS             971..1321
                     /locus_tag="NK788_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCH6769578.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1302..2018
                     /locus_tag="NK788_001240"
     CDS             1302..2018
                     /locus_tag="NK788_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6769579.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2018..3466
                     /locus_tag="NK788_001241"
     CDS             2018..3466
                     /locus_tag="NK788_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6769580.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3536..4045)
                     /locus_tag="NK788_001242"
     CDS             complement(3536..4045)
                     /locus_tag="NK788_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769581.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4035..4760)
                     /locus_tag="NK788_001243"
     CDS             complement(4035..4760)
                     /locus_tag="NK788_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769582.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(4983..7103)
                     /locus_tag="NK788_001244"
     CDS             complement(4983..7103)
                     /locus_tag="NK788_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCH6769583.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7333..8511
                     /locus_tag="NK788_001245"
     CDS             7333..8511
                     /locus_tag="NK788_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCH6769584.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGMLIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            8527..9285
                     /gene="aroD"
                     /locus_tag="NK788_001246"
     CDS             8527..9285
                     /gene="aroD"
                     /locus_tag="NK788_001246"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCH6769585.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9308..9793
                     /gene="ybaK"
                     /locus_tag="NK788_001247"
     CDS             9308..9793
                     /gene="ybaK"
                     /locus_tag="NK788_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCH6769586.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(9864..11117)
                     /locus_tag="NK788_001248"
     CDS             complement(9864..11117)
                     /locus_tag="NK788_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6769587.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11235..12584
                     /gene="gdhA"
                     /locus_tag="NK788_001249"
     CDS             11235..12584
                     /gene="gdhA"
                     /locus_tag="NK788_001249"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCH6769588.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            12696..14042
                     /locus_tag="NK788_001250"
     CDS             12696..14042
                     /locus_tag="NK788_001250"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCH6769589.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14350..14736)
                     /locus_tag="NK788_001251"
     CDS             complement(14350..14736)
                     /locus_tag="NK788_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6769590.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4830 a         2624 c         3009 g         4300 t
ORIGIN      
        1 taagaaaaaa taagcgattt tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc
       61 agatcaaaat acttcaatgc gtggacaatt ccgccttctg tatttttctt tgtgataaag
      121 gtagaaagtt cttttaatcc gtcataggcg ttgcccatag cgattttgtt gtcgcaagct
      181 tccaacaagg caaaatcatt cggaccatcg ccaaatccaa aagtaggaac tttttccagc
      241 tgaagttctt tttgcagacg tttgacacct gccccttttg agattccttt cttcactgta
      301 tcgatcgagt aaggaccatt ccgataaaaa gttagctcag gaaaatgttc gtgatagtat
      361 tcgtcacctg attgagataa gatcagcaac atatttactg tgttttcttt aaagcctaaa
      421 ggatcaattt ccggaacatc tgaatgaatg aagtcgtaag cattgatcat tgtttggcta
      481 tgaccggtac accagatttt ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt
      541 acatgatcat acattttttg acattcttct gaagaaaatg tttgagaata gatttcttcg
      601 ccgtctactc gaatgaaaga accattcatg gtgaccgcag aagtaatgcc cgcatcttcc
      661 atgatagagt ggatttctgc ttctgtacgt cctgtagcga tgacggggag aacattgttt
      721 tcttttaatg cgttcatagc ttttgccact tcgggtgtga tttttgattt cgcatctagt
      781 aatgttccat caagatcaaa gaaagtgatt gcacgataat ttgacacaaa taaaactcct
      841 tttttattgg aataagttta tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag
      901 aattcctgaa catattggtt cagtcaacaa tttagagtat actataagaa gagaatttag
      961 gagggaaaga atgatttttt tcagtattgg tatcttattc ttaataattg gcagtctttt
     1021 tcggattctg ccctcaaaag gaaatctgcc tttttatggg tatcattcgc ctcttgccgc
     1081 aaaaacagac gctcattggc ggctggctca aaagacaagc ggaaactggt tttttcttat
     1141 gggcttactg atggcattga tcggctatta tttaaagaca tctgggcata cgaattattt
     1201 tctgattgaa atgcttctgc tagtatttcc aattatgcct atttttatta tgacagaaaa
     1261 gaaattacag aaatatgacc tggaaactgg agggaacgat aatgaatatt ttaatgattg
     1321 aagataatca atctgtttct gaaatgatgc agatgttttt cttaaatgaa ggttgggaag
     1381 caactttcaa gtatgatgga aaagaagggc tcgatgcctt tttagaaaac ccacaacatt
     1441 gggatatgat tacactagac ctgaatttgc ccacactgga cggtatgcta gtaggaagag
     1501 agatccgcaa agtttcaagt actgtgccga ttatcatgct gacagctaga gattctgaaa
     1561 gcgatcaggt gatcggctta gagatggggg cagatgatta tgtcacaaaa cctttcagtc
     1621 ctctgacgct tattgctcga atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa
     1681 gagaagaagc agcagtttca gatgatcatt ttgatatcga gactagtcat ttcaagatga
     1741 atacgaaaac acgagaagcg tatctggaca accggccaat tgaaggactg acgcctaaag
     1801 agttcgatct attgtttact ttagcaaaaa aaccacgtca agtattctct cgggagcagt
     1861 tgcttgaatt agtctgggat tatcagtact ttggtgatga acggacagtg gacgcccata
     1921 tcaagaaatt acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa actgtttggg
     1981 gagtcggcta caaattcgat gattctggtg ttgcctaatg cgttatttgt atcagcaact
     2041 cttagctttt tgcggaatgg tcgcgttgat cttattgatc gttggagttt cttttacaca
     2101 actgacaaaa caaacgattg aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc
     2161 agttgaaaag acaacgcaaa cctatgccga tacgctcccg cagtttagtc aagatcaggc
     2221 gtttcaaaat gcattgctgc tgacagagca agtactgaca gaacaaaatg tgaactttat
     2281 atttatcgat aaaaacgaac gtgtcattta tccgactaat acagctttac tgaattttac
     2341 cttaacagct gatcaatgga aaaatctgag gaatggacgt caagaaaaat tcacatcgaa
     2401 taaaaatatt ttaggacaag cacaggcgac ctcttacgca cttgtgccat ttaatttgaa
     2461 tcatgaattc tatggagcac ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt
     2521 tcgtccaatt acactgaacc tgttcaaagg atttattttt tccagtatcg ttgcagttat
     2581 tgccagctat gcttttgcag cattccaagt taagcgtatc aatcggttaa ggaatgcgac
     2641 gaaagaagtg acgaatggta attttgatgt acagctcccc gtacatgaca aagatgaatt
     2701 tgatgagtta gcagatgatt tcaataaaat gacgaattca ttgaaagaat ctcaagcaga
     2761 aattgaagaa caagaaaatc gtcgaagaca atttatggca gatgcttctc acgagatgag
     2821 aacacctctt acgacaatca acggacttct ggaagggtta gaatacaatg caatccctga
     2881 aaatcagcga gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt
     2941 ggtgaatgaa aacttggatt atgaaaaaat ccgtacgaat caaatcagta tggtcattaa
     3001 aaaattcaat ggaacagaaa cattgaaaaa tattgttgca cagttagaag ctaaagcaga
     3061 agcagcagga gatacattga cattgaaagc cgttgatgat attgatgttt acgctgatta
     3121 tgaccgattt gtccaaatca tggtcaatat tattcaaaac gcgatacagt ttactgaaaa
     3181 cggccaaatc acgattactt tagaaaaagg ctatcttgaa acgatcatca cgattgaaga
     3241 tacgggaatt ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt attataaagt
     3301 agatccttca agaaaaaata caaaatatgg tgaatcagga ttaggtttac caatcgtgca
     3361 gcaacttgta cgcttgcata aaggaaaatt agaagtagaa agcgaactag gaaaaggaac
     3421 aaaattcgct gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat
     3481 aaaagatcat gaatcgatct tctataccgc ttgtttttac tataaaatga ttgatttagc
     3541 ccgcgggctc taaacgatca atataaggac tttgagcaat agtagcatca tgagtaaaga
     3601 gtaagatacc ttgattttct tcatgagcta aataagaaag caagtggagt agctcttgga
     3661 tctccaatac actaagttca tcaaatgtat cttcaataac aatattgttt cgcttggaaa
     3721 gcaaggcatg aagtaattgc agctttactt tttcaaaaga cgtcaactca gctgccgcac
     3781 aattaagcaa ggaaggagag atatcaagtt tttcttgata ggccaaacga tcttgtttga
     3841 tttctttttc cggtacatcc aacaggacat ggtctttaag attcagataa ggaaacatcg
     3901 gccaattttt ttggatgatc gcaaaattat ccagttttgc aacaagaacc tcttttttca
     3961 agtgctccaa tattttatgc tgtttttcga gaggacacaa aataacggtc aagtgttcag
     4021 catgaatcaa atattcaggt ttcattacgt ccaggcctcc tactttgcaa atgatggata
     4081 aaaaaagtga aagggaatag actaagaatg agacagcatc cactgagccc aataagagtt
     4141 tgggcaaatc ccttaagtat atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa
     4201 aaagtctgtt gatccatcgg aattgtaagt ccgcttaccg aagtcaagat atcacggata
     4261 tctggcagtt cagtagtctt taagacaaga aaattgatcc tttgaagaag cgcttcataa
     4321 actgactgga agcttacgat ccacaataaa accaagatac aacaaagaaa gagaggaaag
     4381 aacagctgcc agaaaataaa aaggaataat ttggttttag aaagaccagt caggcgccaa
     4441 ttgatcaatt cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag
     4501 gcaaatagaa aaaataaact gaatatgcct tttatataaa atcggctagc taattctaaa
     4561 gccggaagaa gctctgtaga tgcagaagcg ccaataaatg aaagccgatc ttggacttga
     4621 ttggctaaag ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa
     4681 aagcttgaaa ataaacaata gagtattgtc tgctttttgt aataaattaa tcgagcagta
     4741 gcatagcgga ttattttcat acttttcacc tctataaaaa gtatagaaaa tgaatatgaa
     4801 taaagtatga aattccttta gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa
     4861 gcaagaggtt gtgaagaagc tgccctattc tattaccgta gatgcaagct tttgaacact
     4921 gtttattcga aaagaggagc agcgacaata attttgtcgc tgctcctttt aaatttattt
     4981 tgtcattcgg aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg cgttttataa
     5041 agatcaacag ttgattgtcc gttgtgttca ctaaagacac tagttgactt gtcaccaaga
     5101 gatttttcag cttcttttaa tttctctaag ccattcttgt agtcataatc actggtatca
     5161 attgggtcta atccactatt cgtatagaac cgaagaagat caccattatt gatttggtca
     5221 gaaacagcta actgtttatc aactttttct ttcagtgaat caatttcatt ctgtacttcc
     5281 tcagtaggtt cagtaatagc ttcaccagtt tcgttactat agatcgtacc accgtagtaa
     5341 gtatattttg gtgtaacaaa atcaccatca cggaaagaaa caagctgttc atgatctttc
     5401 gagaatagat cttgtcctaa ctgaatataa ttttttgtat ctacacctag taagtgaagt
     5461 aatgtaggca aggcatcaac ttgtccgccg taagtgtgat tgattccgcc ctgtgtttgt
     5521 ccagggatat ggatcatata agggacacgc tgcatttgtg cattgtcgta gtttgtccaa
     5581 gtagaacttg tttttccaac taattcagct agatttttat ttcttgaatt cgagacaccg
     5641 taatggtcac cgtaaaggac gatgactgaa ttatcataca agccgctttc tttcaaataa
     5701 ttgaagaatt cttgtacggc ggtatctaaa taattggctg tcgcaaagta accgttgatc
     5761 gtttcatcat ttgtattggc taacgggaat cctgcttcgt cattggtgaa ttttgaataa
     5821 ggataatggt tagatacagc aatgaatttt gaataaaatg gttgttgcag acgttccaag
     5881 tattgtacgg attgttggaa aaatggttta tcatgcaacc cgtattggaa cgaattgtca
     5941 tcattaacat cataataaga agaatcaaag aaataatcat atcctaaatt cttgtatgtc
     6001 tcgtttcgat tccagaatgt accagcgttc ccatggaata ccgcactagt gtaaccttgt
     6061 gtttgactta aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa ttgtgtgaac
     6121 aaggagcctt gattcaagcc aaacaatgaa ttttccatca gcgtttccgc atcgcttgtt
     6181 tttcctgctt ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt gctgtggtaa
     6241 agactattga tgaaaggtgt aacttcatgc tggagaccat tctcatctgt caatttataa
     6301 tcgatcagga attgctggaa gctttctaaa tggatataaa tcacattttt tcctttagcg
     6361 agcccgaaca aatcgtcatt tggtgctgca taatgttctt ttacataatt ttctacttct
     6421 ttcatatcat tgggacttgc ttctgcccgc acttggtttg tctggtaagt ttgaacaccg
     6481 tcataaacca tgaaagcatt caagccaaga tatttgacca aataatcacg agagaatgtt
     6541 cgagtcagca attctggacg atcagcttcc gccataaaaa gatttcctga gaaaatcata
     6601 actgctaatg tagagatcgc aaaagccatt cttgctcgga tcgggcgagg gtccaacttg
     6661 attttttttg caatcagcag aacagtcaag ataataaaat cgatccaata gatcacatca
     6721 tatgggcgaa acagtcgaat ggcactttct cccagaccac tcgctacttt cccagcacct
     6781 aaaatcgtgt tgatcgtaat gaagtccgtg aattctcgat aataagtgac attcgaaaaa
     6841 acaagcaatg tcaaaagaaa ataaatgacc atcatcgtga tataagcaac tctagggcgt
     6901 ctgatataaa gagcaattcc aagtaataac aatgtagttg caatagggtt gataaacaag
     6961 ataaagtatt gaatgccact ttcgattccg agatggaaat caactaaata ggcaaaaatc
     7021 gttttcaacc aaagtaaaac gactagtaaa gaaaaaaatc cgagcctctt attcaaaaag
     7081 gcaggtgttt ttatacattt caagataatc ttcctttctg cacttcttaa tcacagaagt
     7141 gtcatgaaac catgttacac agctcactta ttctcattta ttttactagt atgacaatat
     7201 agtgtcaatt gaaaccagaa gtttatgctg tttcattaag gttttcttta agcttcatga
     7261 aaaaatgagc agtaagacaa gtcctatgtt ttttgttata cttgggaaga gaaagctaaa
     7321 gcaggtgaag gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt gaaaaaagga
     7381 tatccactga tccaaaaaga agaccttgct catccccaaa aagaaaccac gcaatgggtg
     7441 gaattagttg attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa gcagaataag
     7501 ggaatcggtt gggtattgag ccagcagaac atcccatttg atcaatcttt ttttgaagaa
     7561 ttttttgtga agtcaaaaga aaaaagaact ttcttttttt cagatgattc tactaccgct
     7621 tttcgtatat tcaatggaga aggagacgga atcggcggtc tgattgttga ttattatgca
     7681 ggttatgctg ttttttcttg gtataacgaa acgttatatg cacatcgtga attattgatt
     7741 caagcttttc gtgaaatcta cccggaaatc gtaggaggct atgagaaaat tcgatttgaa
     7801 tcagtaaatc tgcctgagag ccagttcatt tacggaaaag aggcaccgga acccttgatc
     7861 gtaaaagaaa acggagtaaa atttgccgtt tatttaaatg aaggattaat gactgggatc
     7921 tttttagacc aaaaagaagt acggggatca ttagcagaag gtttcgcttc tgggaaaaca
     7981 gttttgaaca tgtttagtta taccggcgcc ttttctgttg ctgccgcaat gggaggggca
     8041 atcgaaacaa caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca ggaacagttt
     8101 gaagtgaatg gtttggatgt cgatcagcaa aaaatcattg ttatggacgt attcgagtat
     8161 ttcaaatacg ccaaaagaaa acaattttta tatgacatga ttgtattgga tccaccaagt
     8221 tttgccagaa ataagaaaaa agtctttcgc gtagcaaaag attatgggga actggtcaag
     8281 gatagtctgg atattttagc agaagacggg atgcttattg cgtctacaaa tgctgcgaat
     8341 gtctcgatag aacaattcca aacaatgatt gaagaggaat tccatgccaa aaatgtttct
     8401 tttgaacaac ttgctttata tcgtctgcca aaagatttcc agacgacgga taccttttta
     8461 gaaggcaatt atctgaaagt gtttgtttat caaattaaaa ataaccatta gaaggagcga
     8521 tgcagaatgg tttgggtaaa gggattgaat ataggaagcg gtagaccaaa gatttgtgcg
     8581 cctcttatcg gggccgtccg agaggaagta ttacgtgaag cagttcttgc acgtgaagca
     8641 ggggccgatt tagtcgaatg gcgagtcgat cattatcgtg aagttttttc ttttgatgaa
     8701 gtagcagaaa cattggttct tatccatgag atgcttgatg gactgccgtt attatttacg
     8761 tttcgtacct tagctgaagg cggagaacaa gacatatcga tccgtcagta tcgcgacctt
     8821 tatgaacatg tgatacacac aggattagta gatatggtcg atatcgagtt gttcaaagta
     8881 gaaagtctag gaaaaagact gatagaggaa atcaagtctt taaacattcc tttaattatc
     8941 agtagtcatg attttaaaga aacaccagca gaccctgtct tgctttaccg attgaatgtt
     9001 atggaacatt ttggagccga tatcggaaaa ctggcagtaa ctcccaataa tgaacgagat
     9061 gttttgcggt tgatggagtt gactagacgg gcaaatgctt ttgtctccat gcctattatt
     9121 acgatgtcca tgggaaatct tggtaaaatt tctcgtctgg ctggcggaac gacgggttcc
     9181 gttatgacat ttggttcctt aagcagcgat tctacttctg ctccaggaca attaccggtc
     9241 aaagcattga agcaaattat tcaattatta aatgaaccgt tgtagaacaa gtttgaggtg
     9301 atattttttg gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg tcgaacaaaa
     9361 aaagattccc tatacagagc acgaatatga gtgggacgaa agccacctaa gtgcgtcatc
     9421 ggtcgctgaa cagcttccag aaagccaatc tcgtatattt aaaacactgg ttgctgtggg
     9481 caatgtgacg gggcctcttg ttgctgtgat accgggagaa gccgaattga atctaaaaaa
     9541 attagcaaaa gtttcaggga ataaaaaagt agaaatgctc catttaaaag atttggaagc
     9601 aacgaccgga tacatacgtg gtggctgctc gcctataggt atgaaaaaac tatttcctac
     9661 ttatttagat caaattgcag aaagctatga acaaatcatt gtctcggctg gcagaagagg
     9721 gctacagatg gagttagcac ctcaagatat ctgtgcatta acgagcggac aatttgcaga
     9781 tataaaacaa taaatcatac gtataaaaga aagaaggagg ccgggatgtt gtttgtccag
     9841 tctccttctt tcttttttat acattatgct ctttgcccgg cattcattcc tgctacatgc
     9901 cctgtagcga aagccgctgt gatattaaag ccgccagtat aaccattgac atccagtaat
     9961 tctcccgcaa aatacagacc atcaatgatt ttactctcca atgttttagg ataaatttcc
    10021 tttagagaaa cccctccgcc agttacaaat gatttctcaa taggaaaagt tttgttgatt
    10081 gatagcttaa actctttgca tagttgaaca aaatcttgaa tttgtttttg agaggcttgt
    10141 tgtcctgtca gatgatccat ttcaagccgc tcaagaaaga aaactaacaa tcgctcaggc
    10201 aatataccat gccaagcatt gaccaagttc tttttggttg ctttactttt ttctgtcaat
    10261 atatgaataa gctcttgttt ggtttgtgta gggaaacaat ccagagaaag tgtcactggt
    10321 tctcccgttt tctctaattc tttattgaca aaacttgaac aacgcagtgc agccggacca
    10381 gaaataccga aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac acgacctttt
    10441 ttatttaaaa cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt tttctcctga
    10501 atatacggtt catccgatat caagggggac tctgtcgcat aaagaggcgt caccgtgtga
    10561 cccactcgct tcacgatctt gtacccatct ccagtagaac cagtagatgg ataagtccgt
    10621 ccaccagttg ttaagataat ggcaggagca tggaactctt cgaattccgt gcgtataccg
    10681 taaattttat gttctttgtg gatcaatttc tctactcgag tagagaatag aagcgtcaca
    10741 tctagttctt tcagacgatt gagcaatgct tcgataatcg tttttgattt atttgttaca
    10801 ggaaacatcc gtccatgatc ctcttctttt aaatgtacgc cttgtgattc aaagaagttc
    10861 atgatatcga aattattcca ttgcgcaaac gtactatata aaaactttcc atttccagga
    10921 atatgtgcaa tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg accaccacct
    10981 gtcatcagta gtttttttcc tgcttttttg tttttttcga tcaagagtac gcgtgcgcct
    11041 tgttcagctg cactgatcgc tgccatcatc ccgcttgttc cagcgccgac tacgatcaca
    11101 tcatacagtt tttccattcc ctcacctcaa ggaaagtgta gcatatctga agaaagaaaa
    11161 cagaaaaaaa atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa
    11221 aggagcgtgg aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac
    11281 atcaaaagga tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg
    11341 ttatgtcttt tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa
    11401 cggaaccaga aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc
    11461 gagtaaatag agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat
    11521 tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt
    11581 tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc
    11641 ctaaaggaaa atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt
    11701 caaaacatat cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag
    11761 aaatcggcta tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga
    11821 caggtaaacc tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat
    11881 tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag
    11941 tttttgtttc cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag
    12001 gcgggaaagt tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag
    12061 atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa
    12121 accaacgtcc ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag
    12181 cggatatcgc tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct
    12241 tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag
    12301 ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg
    12361 gaggagtagc agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa
    12421 gagagcaagt ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc
    12481 aaacagcagc cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat
    12541 ttgaaaaagt tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt
    12601 ggaaatattg tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta
    12661 ttacctgtag agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt
    12721 caaaagtagc accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag
    12781 cagcccacaa cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg
    12841 atttaccaac gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa
    12901 tccaatctga ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc
    12961 gtgcagcgat cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa
    13021 aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga
    13081 tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta
    13141 ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag
    13201 aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag
    13261 aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct
    13321 ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat
    13381 tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg
    13441 aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt
    13501 tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg
    13561 gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg
    13621 atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg
    13681 tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg
    13741 gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa
    13801 cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg
    13861 atgcttatac attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt
    13921 acttgaatgg aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt
    13981 tcgcacttct aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt
    14041 aagataaggg tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa
    14101 tgaaaactgg tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg
    14161 ctgtgacaag acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct
    14221 ctttagtttg ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt
    14281 ttttgttttc tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat
    14341 attcttagct taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt
    14401 aattactttt ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc
    14461 ggggtaagca gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct
    14521 tttttcaccg ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc
    14581 cccattatct gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata
    14641 atctttacct acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat
    14701 caatatgata acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttta
    14761 tta
//
LOCUS       DAHUWM010000060        14703 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHUWM010000060 DAHUWM010000000
VERSION     DAHUWM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.060"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(33..746)
                     /locus_tag="NK788_001252"
     CDS             complement(33..746)
                     /locus_tag="NK788_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6769591.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            921..2345
                     /locus_tag="NK788_001253"
     CDS             921..2345
                     /locus_tag="NK788_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6769592.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2644..3351
                     /locus_tag="NK788_001254"
     CDS             2644..3351
                     /locus_tag="NK788_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCH6769593.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3338..3661
                     /locus_tag="NK788_001255"
     CDS             3338..3661
                     /locus_tag="NK788_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCH6769594.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3750..4787)
                     /locus_tag="NK788_001256"
     CDS             complement(3750..4787)
                     /locus_tag="NK788_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6769595.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5108..6091)
                     /locus_tag="NK788_001257"
     CDS             complement(5108..6091)
                     /locus_tag="NK788_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCH6769596.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6506..7276)
                     /locus_tag="NK788_001258"
     CDS             complement(6506..7276)
                     /locus_tag="NK788_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769597.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7269..8204)
                     /locus_tag="NK788_001259"
     CDS             complement(7269..8204)
                     /locus_tag="NK788_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769598.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8520..10169)
                     /locus_tag="NK788_001260"
     CDS             complement(8520..10169)
                     /locus_tag="NK788_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCH6769599.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10286..12889)
                     /locus_tag="NK788_001261"
     CDS             complement(10286..12889)
                     /locus_tag="NK788_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCH6769600.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12901..13950)
                     /locus_tag="NK788_001262"
     CDS             complement(12901..13950)
                     /locus_tag="NK788_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCH6769601.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13976..14290)
                     /locus_tag="NK788_001263"
     CDS             complement(13976..14290)
                     /locus_tag="NK788_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCH6769602.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14306..>14703)
                     /locus_tag="NK788_001264"
     CDS             complement(14306..>14703)
                     /locus_tag="NK788_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6769603.1"
                     /translation="SDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIGKN
                     IAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRILAE
                     LSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4354 a         3052 c         2486 g         4811 t
ORIGIN      
        1 aaataagctc tgaaaaaata attttcccga gcttattttt caacaaaaac agcttcaaaa
       61 acaaagtctt gatataaatg tctcgatacc gaatactcga aaggtttacc agacgataga
      121 gcaacaactt tgtctatctc aatcataaat tcacctggat ctatttttaa tatttttgcg
      181 tcaaattcat tcgcagtatc tcctttgata cgtatgtgcg aagtctttat ttccagaccc
      241 agttcatttg tgatataagc atatacagat ttctttaaat gttttggttc caagcctggt
      301 atgactgcta atggcatgaa tgtttgctca atcgagtatg gttgacaatc aataaaacgg
      361 cttcgtacaa tattatatac atagtctttt acactgatac ttaatttatc agctactttt
      421 ttcgatggaa gttcaattga gaaagtatgg atttctgaat ctacgtgttc ttcaccaacc
      481 acgctaaaca aaccttcgtt tggaccaaga actcgtgagt tatcaattgg tgttctgacg
      541 acttcagtcc catacccact tctacttcgc aaaactcctt cggaaacaag taagtctacg
      601 cctttcttta ctgttaactt gcttgtatga taaataactg ccaattcatc tcctgtagga
      661 agtttctcac caattttata ctctccattt acaatttttt tctttatatc taagtaaatt
      721 tccatatact tcgcaatttt tttcaatggt gaccctccaa tcaaaaacat atcttcatct
      781 atactttaac ataaataaac cgataaaatc cacatcaaaa ttatacacac aataaaaaag
      841 atatatatat ttaaacaaaa ctatttactt taagttgtaa gcggtgttat cattaagtgg
      901 aaacaggagg taaaaaaatt atgacaacta tgaaaaagct aacttttcca gatgattttt
      961 ggtggggttc cgcttggtca gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag
     1021 agactatctg ggatcgctgg tttaaagaac aaccttaccg gttttatcaa cgaatcgatt
     1081 cctctattac aaccgatcac atccatcaat ataaagaaga tatagaacta atgaaaaaaa
     1141 ctgggcataa ctcctttcgc gtttctattt cttgggcacg aatgtttcca aatggcggta
     1201 ccggaaaagt aaatcccaaa gccatttctt tctatcgaaa attattttcc gaaatgaata
     1261 ataacggaat caaagtattt gccaacttat atcattttga catgcctgct actcttcaag
     1321 ataaaggagg atgggaatca cgtgaagtag ttgatgctta tgttcattac gctgaaactt
     1381 gttttaaaga atttggtgac ttagtttgtc attggtttac cttcaatgaa ccacttgggc
     1441 ctattttagg tacttatctt gaagattttc attatccaaa cctcattgac tttaaacgcg
     1501 gagcgcaagc agcattcttc actatacttg cacacgcaaa agcaatacaa gtatttaaag
     1561 aactagctct tcctagtaaa ataggcgtta ttctaaattt gagtcctact tttcctcgta
     1621 gtcaaaatcc tgctgatgtg aaagcagcag aaatcgctga tttattttat acgcggagct
     1681 ttttagatcc tatggttaaa gggaattttt ctaaaaagct tatcctgtta ttacaagaat
     1741 ataatcagct tcctgaagat tatacgaaag aagatcttgt atgtatctac gaaaataccg
     1801 ctcaaattct tggactaaac tattacgagc cacgtcgcgt caaatctcgt tcttctatgg
     1861 tggatacatc tagtccattt ttaccagagt ggtttttcga accacatatc atgcctggac
     1921 gacgaatgaa cgaataccgg ggatgggaaa tttacgaaaa aggaatttat gatttgtgca
     1981 tggatatcaa gaaccattat ggaaacatcg aagcatttat ctcggagaac ggaatgggcg
     2041 ttgcggatga agaacgtttc atggatgata cagggcaaat aattgatgat tatcggattg
     2101 agtatatcaa agatcatctt gcttacttat ggaaggcaat aaatgaagga tgtaacatta
     2161 aggggtatca tctttggaca tttattgatt gctggtcatg gatcaacgcc tataaaaacc
     2221 gatatgggct ggtttcactc gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg
     2281 aattttacaa gcaattaagt gatacaaatg gatttagata cgatgtagac ttgttagttg
     2341 aataatattc taaaattcca cttgaagtga acacattaga aaacaagaat aatgagaaat
     2401 accaataaca aatacttttt cttcatactt catttatact tgtcagtata agagactgtg
     2461 acaaatgtag tgatcctcag aagttaggtc tggaaatcta acttgagaag atttttttaa
     2521 actacgtcca gtctttttta tttgtttaaa attgaagttg acaagtcttc attaaaaatt
     2581 tagaatattc acaagtttga cgacaattat gaaaatttat gtcgtgctaa aggagttttg
     2641 ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttt
     2701 ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg gtttcatccg
     2761 ctggtcgtca cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt tatcacggtc
     2821 agcatgcttg ctagccatag tccactgctc tccatcgttc tctcgacctt tctagtcaat
     2881 tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt
     2941 caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa
     3001 ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct
     3061 tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat
     3121 ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt
     3181 caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact
     3241 ttgattctgg tatatgtcgg actgattttt atcccaagta acttagtgat tttagttgta
     3301 acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt
     3361 gctgactatt ttaggttgta ctttagcaac ttggctttct aggatcctac catttgtctt
     3421 actgaaaaaa ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt
     3481 cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca
     3541 gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa
     3601 aagcttattg gtgatcgtct tggtaggaat tctttccctt tcgttgattc gcttattata
     3661 aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc
     3721 aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact
     3781 tttcctaata tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc
     3841 atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca
     3901 gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg
     3961 aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc
     4021 tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg tttgatttga
     4081 tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat
     4141 tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt
     4201 ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc
     4261 gctagttgat tcaaaatcga accgacgccc ccagctccgt tgatcactaa gatttttttc
     4321 ccgcggttgg cattttcttc tggagtcaga ccaaattttt caaataacaa ttcataagcg
     4381 gtcaatgagg tcaacggcaa ggctgcggct tcttcatctg aaagattttt tggtgcaaga
     4441 gcgacaatac gttcgtcaac cagttggtat tcttgattgc ttcctgctct tgtcgttgta
     4501 cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcatcacc tacagcaaca
     4561 actttcccga ctccatcaaa ccctaagata cgtaaagcat ttcttatccc gttatcttgg
     4621 cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt
     4681 gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcgac
     4741 aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct
     4801 tagcttcatc aagttggagc atccatacag ttttcatttc aatatatata gtatgctatt
     4861 tgttccactt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
     4921 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttttaaac ggtgttcaat
     4981 caactgaccc tcggtattcg ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg
     5041 ctgttcttat tacttgatgc aggacagctt tttcacgacc tctaattcac ggtgttcaag
     5101 caagaaatca ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct
     5161 ctcgtacttc atcagtagat ttcgtattca tcaactgaac tcgcaagtca tttgctcctg
     5221 gaaatccttt gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga
     5281 tttctgaata atgatcttgc agatctagtt gcagacgaag tagtccgatc agttcttttt
     5341 ctgtatgttc cctcggttct ttttcaaatg cataaggatt cttgaaaatc ccccggccaa
     5401 tcatgatgcc atctacgcca tatttttcgg ccaattcttc tcctgtctgg cgatctggga
     5461 tatctccgtt gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt
     5521 tagggatgac ttcccaatgt gcatctactt tactcatctc tttacgcgta cgcaaatgga
     5581 cagaaagatt ggctatatct tgatgaagta ggtgactgat ccattcttcc atctcagaca
     5641 tttccgtata gccgatacgc gtcttgacgc tgacaggcaa accgccagct ttagccgctt
     5701 caatcaattg tgccgctact tctgggcgaa ggatcaagcc actgcctttt ccacgttctg
     5761 ctacattagg gactgggcag cccatgttga tatcgattcc ttggaagccc atttctgcca
     5821 tagcaatgct catttctcta aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg
     5881 gctgttcatc ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca tctggatgac
     5941 agaaactgtc tgagtttgta aactcagtaa agaagacatc cggtgctgct gcatgcttta
     6001 cgacatggcg aaatacaaca tctgttacat cttccattgg tgctaaaaca aaaaaaggct
     6061 ttggcagctc tttccaaaaa ttagtagtca tttatttgtt actctccatt ctatcaagca
     6121 gtgaagtcgc aatctttctc aaaaacattg tcattatact aggaaacaaa aaaagaagca
     6181 aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa agactgtgac aaaaatctta
     6241 tcacaattcc ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg taataagtcc
     6301 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga
     6361 acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc
     6421 tcaacaaacg acaaaatatg aggaactatt ttcccgtttg tttctctaat actcaggtca
     6481 aaacgctgat ttcacaacct cttttctaac ttctcaatcc tcttccttta ttgatcaaat
     6541 accaagtaac ggcgtacaaa acaacgatga atagcacaag tacgatcatc gatgttgcta
     6601 ctggcacatc gattgttcca aggaatccgt aacggaaacc ggagatcata taaacgatcg
     6661 gattgacttt cgaaacagct tgccaaaatg gcggcaacat agagatggca tagaaaacgc
     6721 cacctagata agttaacggc tgcaagacaa atgtaggcac gatcgataca tcgtcataag
     6781 actgagcaaa aatcccgttg agcagtcctg ctaacgaaaa caagattgcc gtcatcaata
     6841 atgtaatgat gaccatgaac catgaataca catgaagtgg tacaaagaac aaggaaatga
     6901 tcgttacgag agagccgacg agaatactgc gacccactcc gccaatcaca aagccccaaa
     6961 tgatcacatg cgtaggaaca ggcgctacca agatttcttc gatgtttttc tggaattttt
     7021 gagagaagaa ggacgaagaa acgttggcat acgaactagt gatagcagac atcataatca
     7081 aaccaggcac gatgaattcc atatatgaaa aaccacccat gtcaccgatc cgaccaccga
     7141 tcatttttcc gaaaataaca aagtacaagg atgtcgtgat cacaggcgga accagtgttt
     7201 ggacccagat acgcaaatag cggttcgttt ccttgatagc taaacttttt aatgcagtaa
     7261 aataaagatt aaacattttt ctcctccgta tgattatttt cttctgtgat tttcaaaaac
     7321 agttcttcta aacgattcga cttattcctc atagaaagaa ctttgattcc ttgtcttgtc
     7381 agctgatcga agattccgtt caccccttga tttcgttcaa cttcgacagc gattgtctgt
     7441 tcatcttcaa atgtacaagc gtatccttca atcacaggtt tggtctcata tggcgctaaa
     7501 tcaaagataa atgtttcaaa ttgcagtttg gatagaagct ctttcatact agtattctcg
     7561 atcagttcac ctgattggat gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa
     7621 taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca attctcggag aaactcccac
     7681 atctcgcgtc tcaattcaat atcgactcct gctgttggtt catccaagat cagcaaacgt
     7741 ggttcatgca tcaatgccct agcaatcatc agtcttcgct tcattccccc ggaaagcata
     7801 cgtgcacgga catttctctt ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt
     7861 ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt gattcacgac gatttgctgg
     7921 accgtttcaa agggattgaa attgaactct tgcgggacta atccaatctg ttgctttgct
     7981 cggactagat ccgtatccag atcataaccg aagacattca cttttccaga tgttttattg
     8041 actaaagaag taatgattcc gatcgttgta gacttcccag ctccatttgg tcctaatagc
     8101 gcatagaaat ctccttcttt cacggaaaga tcgattcctc gcaatgcttc gaccccactt
     8161 ccataaacct ttttcaaatt atttatttca agcgcatttg ccatttattt cctatctcct
     8221 tatagacatg attctttatt gtatcatgga atacagcaga tgaaaaaaca acttggttcc
     8281 agcgaaaaat caggttgctg ggctcaggaa aatctcctaa tgaaagacat caatcggacg
     8341 aagtcagaca accttttcag aaaaaacaaa tcgaagataa aggattacta ttcatatgtg
     8401 caacaaaagg agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta
     8461 taaacggcgg aagcaaaagc caagattttt gatccaataa atactttcga tcacttcgcc
     8521 tatcccaata catgccactg ccgcttttca tcaaataacg cggcttccgt ttttgcggtt
     8581 tctactaaag tctctgggta atccaaaact ttcagcaagg caatcgcatt tcgggtcctt
     8641 gaaggtcctt cttttaattt gaaatcaaaa ctgattcctt ttccttctgt tacttgttct
     8701 tcaaagtgga cattctcaca ataattcttt aaaatctctg tcaattcgat atcatgtgtg
     8761 gcgacaaatg ctaaagatgg atactccgca agccaccgaa cgactgaagc ggaagaagca
     8821 atccgttcga tcgtatttgt cccttttaaa atctcatcta cgaagcagta gcagcgttct
     8881 ttggaagcca cttggtccaa caaacgtttg atcgatttga tttctgaaac aaaataactg
     8941 tcgccttcaa ataaatcatc ttcgattgcc atggaagtca acacatgtcc aggttgcagg
     9001 gtaaactgtt ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct gatggctata
     9061 cttttgacat acgtagattt tcctgatgca ttggaacccg tgactaatgt attcttctgc
     9121 cagttgaccg gattcactac cgcttctttt aacaatggat gatagatatc tttcgctgtc
     9181 acaccgccct cagtaaactc tggtcggcaa gtgatgggca tataagtacg gtaattcaat
     9241 attgcagcgg ctacttctaa ttctcccagc aattcccaca gcttgatggc ttcagttgaa
     9301 tgcttttcta atttagtgag aacgaaatga taggagataa atggcagcat gaacatgatg
     9361 ttgacatatt caaaaatcaa ctctgcttca ctcccactct tcatacgaaa tgaaaaacct
     9421 aatttaggga tgtctcttaa tggtttagtt tgctgtttca gctcttcttg aagtggtgtc
     9481 ggcagttttg ctagctgatt ggcggctgaa atcgttgaaa ccaaataact catgctattc
     9541 agctctgttt ctagtgtttg tttcttcact agataataga ccgtattgaa tactaaccca
     9601 ccgattgcca aatagattcc tagtacttgt cctaaaagaa gcaaacccaa acccaccagc
     9661 ggaagcaagc caagaaaaat aaataaacct acgcgtccaa tctttttact ttttccatcc
     9721 gctagatatt gttttgcaaa attatgatct ttttttccta aacgagcgaa ttgatattgg
     9781 attttttctc tcagttgagg attttcctga taaaaagcaa tcagtttttc taactgctga
     9841 tcttcaccaa aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc tacactagaa
     9901 taggtactgt tgattccttc gaatacttcg aacatatcca ggtcatacca agtaagatca
     9961 tcgatttcgc tatcccatga acggaacttc ttctccgtga gccatgcttc cttcaagctt
    10021 tcttctttat caaatctcgg ttgataagga attttttccc atttgctacg aactgtttcc
    10081 tttaatttca aacgtgaaaa aatgtctaaa gcaactaata aaataacagc tgcaataata
    10141 attaaaacga taaattctct ttgacccata tccctctccg ttcctttatt tgttagcttt
    10201 agtatagcac tgttgcgcta tttgaaaaga gggtaaaaaa acacctttca aaataaaagg
    10261 tgtttttttc ttgtatatta catatttatc tctcatttat caaaattgac aaataatcat
    10321 agggaggaat tgttgttaat tcttgtttat tctccagtgc attgacaact gtcccaaggg
    10381 aaaggatagc ctcaggtact attttttctt gttctgttgg attagctaag cgaagaacaa
    10441 atccttgttc atgaaaataa gaaggataaa tcgatgatac aattaagtct tcaggtaatt
    10501 caatcaatga aaatttacga ggaacatcaa gttcattttg taatggccaa atcttgttat
    10561 ctaacctgtg aataaataca ttcagtttct gcttttgata agaaatagat tgttctaacc
    10621 gtttttctgc ctgttttgcc actttgaact cattaaatct tccttcttct acattaaatc
    10681 caaaagtgac tttatactca cctatctctt gagctagcgg tgtttccatc atgacatgcc
    10741 cttcatttgt cgtatctcct gaagctcgcc caggtcgcca agcaagatta ggttttccaa
    10801 gttcccctgt tgttgaaaat aaggttatga ttaattgatt ttctatacgt tcatactctt
    10861 ttaaaccatc tgcataaaac gtaaaataat gatcattttc tgccacagag acactcttat
    10921 cgaatatttc taaattaaca ggtttctcta ccatagtttc tgaccaattc ggaggaatga
    10981 catcaggggt attttcaata aatccgtttt gaatttgtgc aattgatttt tcagatttat
    11041 ctaaaacatt aattgctaag cgcatacgat gcgagtaaac agtattatga attttgagtg
    11101 tgccttcaac gatttgactt tctcccagac tgaacgatat ttcataagat atttcttcca
    11161 tttcgggttt ttcagataaa cgatctttta aatctttagg aagttgagct tttccgtaaa
    11221 caactaatgt ttcttgtaaa gaatctttat atactttagc tgtttctaat tttagagata
    11281 gttctgtatc tccttctaat ggtgaataat catacgtatc gccatcattt gctgaatcga
    11341 tcaaatgaac gaaatcaaga tattgtctat ctccaactat caaattcaat tgcccatctt
    11401 taaaagaaag tttatattta ttgttactta tctcagtttt gtctatctcg attgctgttt
    11461 ctaatttttc attatttttc tcaaattcaa caacagtgta tcctagtgct ggcacattta
    11521 catttatttt tatgtcaagt tcataataag ctggttcatc aaaatattcg aaacctgtag
    11581 caatcatccg ataagcattt ttccgttctg gaaaataaac ttctttttca atgatattat
    11641 agggactacc tttgaagtgt attttctttg aatcagctac tacatgcacg attttcgatc
    11701 cactataatt ctcagtcgtt gtgttgaaaa caaggacttc attcgatttt agctttaatg
    11761 catcagccat ccgttttaca ataaggtttt cgattccgtc agctatctca ttagcttctt
    11821 tgattcgatg gaaaatatct tccgcaacat tatctgaaac acagccacca atactatcat
    11881 gagcctggtt ttgcaaaatc tttttccata accgacgaag taggccttta ctgatatgga
    11941 taccatttct ttctgcaatc agcatgagcg gctcaactcg ttttagaatt ttttgttcca
    12001 atttaaaatt ttctattttc atttttgttc ttactgagcc aatagaacga tgaacacgag
    12061 agtaaaccgg ctgacgaagt tcacctttat attcaggtaa ttcttttttg tttctgataa
    12121 cttctacata agaaggataa tctgaaataa tattgtggaa tttactgtat ttattcaact
    12181 tctctacgac ttgatcaaaa tttaaaatca ttgacttttg atctacaccc gaaggtacta
    12241 aaatatcttt gttttttcct tgcatatgaa gcatttcaac tgcttcatcc aacttttctt
    12301 ttgcaaattc aggaatatct ttaattgcgt ctaaggtcat cattccagtc atataaccaa
    12361 atggaaaatt catggcataa acaaatttat ctcccaatcc tttccagata aaataaggag
    12421 atggaaccat cgtttcaaag tttaatcctc gccaacacat gaaattatcg atttctactt
    12481 gatgcaaaag tgtaggcaaa ttcgcattga acccaaacgt gtcaggcaaa taccctacca
    12541 tcattggatc tccatattta aggatcgtat cactgattcc aatcattaag tttcttaaaa
    12601 ttgattcagc atccactaat aaggcatctg tttgtgcata ccacggtccc acgaatagtt
    12661 gtcctgtttt catcagtcgt tggataacct taagcttttg cggattgatc tccaaatact
    12721 catcgacgat tgaagtttgt ccatctaaac aaaagtttac tttagggttc ctttccaatt
    12781 catctagaac ttcagtaaag acctgatcag acaatacgat ggcatcttca tttgtaaaat
    12841 accattctct gtcccagtgc gtgtgttgaa ttatatgtgc atttaccata tcagattctc
    12901 ctactttcct tcttctactg aatttacttg ttgttctttt ttatacttga tggattttag
    12961 tgcattgacc attaacgctg taaccactcc cccaataaca attgatacta aatataaagg
    13021 tatattcgta acaaaaggaa ttgccataaa ggttgaaagc attgtttctt gagtaacacc
    13081 aaaaaatcca tttagaccac atgctactgc agtgcccacc acgatgcttg gtattactcg
    13141 taaaggatct ttcacggcaa acgggatcgt cccttcagta atccccacaa ggcccataat
    13201 catagctgtc tttgcattat ctcgttccga ttcatcataa agttttttgt ttaaaattgt
    13261 tgctaaaaac attccaaatg gcggaatagc aattcctaat aacatggctg tactaggtcc
    13321 aaagcttccg cttgaaaaag aagcaacccc aaaagcatat gcgattttat tgataggtcc
    13381 tcccatatca aacgccatca ttcctcctaa aatagccgac atcaaagcag catttgctcc
    13441 tgacatgcct tctagccaat tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag
    13501 tacataccat aatatgattc caacaactaa tgtagtaaca aaaggaatca gcataattga
    13561 ttttagacta ataacttgca tcggtacttt tacttttttc aatgcattag ctaaatagcc
    13621 acaagctaat ccagcaacca ttcctccaat aaatcccata ccaagatttt gacacatcat
    13681 cccagtgata aaagctggtg ctagaccggg acggtcagca attgaatatg cgatataggc
    13741 tgaaatcatc ggtacgataa actgtaatcc cgtctgacca atcattcgca ggactcccca
    13801 gaatgtatcc gtctggtccc aaacacctgt gccaccaaaa atgactgcaa tagaagttag
    13861 taaccctgct gctacgacaa gcgggatcat ataggaaacc ccactttgga aatgatttag
    13921 aatttctctt ccttgtttct tgaattccat tagtaactcc cccacgatat attttttatt
    13981 tgttaattaa tgcaattgct tcatcaatcg tctcttcacc atcttttact acctctgagg
    14041 tccctactgt taaaatcggt ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta
    14101 ctgcaaatat gacaacatct gcctcggaaa catccgaaat tgaaagtttg ttttctatcc
    14161 ccatagcccc ctgtgtttct actttaatat ctaacccttt cttcttcgca aacttttcta
    14221 aattagcttt tgccatataa gtatgggcaa tccctgcgat acatgctgtt actgctacta
    14281 tttttgtcat atgaaattcc tccttttaaa atgatagagc ttttaccaat tcttttttat
    14341 ttctcgaatc ttttattctt ttaacaaact cgtcatccat caatcttcca gataattctg
    14401 ctaaaatacg taaatgatta gtacctttat cagtgtcttt tattgctaac aaaaagacga
    14461 gatcaactgg ttgatcatct aatgcgcccc attcaacaga ccgctttaac ttggctacag
    14521 ccactgttga ttcaattact gctaggcttt tgccatgagg aatggcgata tttttcccaa
    14581 ttcctgtggt catatggttt tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag
    14641 acacaaaccc attagtctct aacaatttcg ataatttttc gactacactt tttttatcag
    14701 atg
//
LOCUS       DAHUWM010000061        14167 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHUWM010000061 DAHUWM010000000
VERSION     DAHUWM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.061"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(38..862)
                     /locus_tag="NK788_001265"
     CDS             complement(38..862)
                     /locus_tag="NK788_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCH6769604.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(835..1515)
                     /locus_tag="NK788_001266"
     CDS             complement(835..1515)
                     /locus_tag="NK788_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCH6769605.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1538..2476)
                     /locus_tag="NK788_001267"
     CDS             complement(1538..2476)
                     /locus_tag="NK788_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCH6769606.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2721..3584)
                     /locus_tag="NK788_001268"
     CDS             complement(2721..3584)
                     /locus_tag="NK788_001268"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCH6769607.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3726..6299)
                     /gene="mprF"
                     /locus_tag="NK788_001269"
     CDS             complement(3726..6299)
                     /gene="mprF"
                     /locus_tag="NK788_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCH6769608.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6440..7246
                     /gene="proC"
                     /locus_tag="NK788_001270"
     CDS             6440..7246
                     /gene="proC"
                     /locus_tag="NK788_001270"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCH6769609.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7325..8881)
                     /gene="rny"
                     /locus_tag="NK788_001271"
     CDS             complement(7325..8881)
                     /gene="rny"
                     /locus_tag="NK788_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCH6769610.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9292..10341)
                     /gene="recA"
                     /locus_tag="NK788_001272"
     CDS             complement(9292..10341)
                     /gene="recA"
                     /locus_tag="NK788_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCH6769611.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10450..11691)
                     /locus_tag="NK788_001273"
     CDS             complement(10450..11691)
                     /locus_tag="NK788_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCH6769612.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11931..12509)
                     /gene="pgsA"
                     /locus_tag="NK788_001274"
     CDS             complement(11931..12509)
                     /gene="pgsA"
                     /locus_tag="NK788_001274"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCH6769613.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12718..13641)
                     /locus_tag="NK788_001275"
     CDS             complement(12718..13641)
                     /locus_tag="NK788_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769614.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4052 a         3154 c         2472 g         4489 t
ORIGIN      
        1 aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg
       61 caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga
      121 ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta
      181 gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac
      241 cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca
      301 tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta
      361 gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata
      421 aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg
      481 taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc
      541 tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga
      601 tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa
      661 cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg
      721 ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca
      781 cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga
      841 attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc
      901 cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac
      961 atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt
     1021 tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat
     1081 tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc
     1141 tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca
     1201 gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc
     1261 attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt
     1321 tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac
     1381 caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac
     1441 atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat
     1501 ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag
     1561 ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag
     1621 tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct
     1681 cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca
     1741 caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg
     1801 aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag
     1861 tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt
     1921 caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt
     1981 tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc
     2041 aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt
     2101 ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt
     2161 tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca
     2221 cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg
     2281 gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg
     2341 taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt
     2401 ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca
     2461 aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat
     2521 taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg
     2581 atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag
     2641 ccatttcact acaataaaaa actgaagaat accattttag gattcctcag tttttcatca
     2701 cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa
     2761 ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg
     2821 gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg
     2881 atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt
     2941 ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga
     3001 tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt
     3061 ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct
     3121 ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc
     3181 aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct
     3241 cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc
     3301 ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag caataaagca
     3361 gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg
     3421 attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc
     3481 accatttcca actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt
     3541 atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc
     3601 gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt
     3661 ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt
     3721 gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt
     3781 cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca
     3841 aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag
     3901 gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa
     3961 aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc
     4021 attttctcgt atacaataaa cagattggct aacagatagt cagctaattc ctcagattct
     4081 ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg
     4141 atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga taatacatat
     4201 gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt
     4261 gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg
     4321 tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct
     4381 gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga
     4441 agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct
     4501 gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct
     4561 cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg
     4621 tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg
     4681 cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc
     4741 gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa
     4801 ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt
     4861 attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa
     4921 aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga
     4981 taaaattctt ttctggataa ccacacagct aacagaatca agacatatac caatatcaac
     5041 tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta
     5101 ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag
     5161 ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga
     5221 aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc
     5281 gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga
     5341 atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc
     5401 gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca
     5461 tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt
     5521 ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc
     5581 gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct
     5641 cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg
     5701 aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta
     5761 ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca
     5821 tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt
     5881 gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga
     5941 cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca
     6001 gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc
     6061 gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc
     6121 aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca
     6181 ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt
     6241 tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca
     6301 ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt
     6361 aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa
     6421 tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc
     6481 tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag
     6541 tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag
     6601 taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat
     6661 tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc
     6721 ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt
     6781 acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa
     6841 tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag
     6901 cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc
     6961 cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc
     7021 caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct
     7081 ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac
     7141 tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat
     7201 caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt
     7261 tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt
     7321 tttattattt agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc
     7381 caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt
     7441 ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg
     7501 cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac
     7561 ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg
     7621 ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga
     7681 tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa
     7741 cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac
     7801 gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa
     7861 cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga
     7921 tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc
     7981 cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac
     8041 gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc
     8101 ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat
     8161 cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac
     8221 ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg
     8281 ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt
     8341 tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag
     8401 tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt
     8461 ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct
     8521 gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt
     8581 catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt
     8641 ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct
     8701 tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga
     8761 ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca
     8821 taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca
     8881 taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa
     8941 ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact
     9001 ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta
     9061 cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt
     9121 taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta
     9181 tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg
     9241 tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc
     9301 tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat
     9361 accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat
     9421 atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc
     9481 accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga
     9541 gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt
     9601 ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc
     9661 tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt
     9721 gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc
     9781 gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg
     9841 tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa
     9901 agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc
     9961 gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac
    10021 acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt
    10081 tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact
    10141 ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac
    10201 atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa
    10261 tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc
    10321 tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat
    10381 ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat
    10441 tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc
    10501 attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc
    10561 tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc
    10621 agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc
    10681 catctgctcc acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat
    10741 ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc
    10801 agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc
    10861 tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt
    10921 atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc
    10981 taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac
    11041 ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt
    11101 tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag
    11161 tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg
    11221 tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa
    11281 agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga
    11341 ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt
    11401 tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt
    11461 tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt
    11521 ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata
    11581 ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac
    11641 gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg
    11701 ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag
    11761 ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa
    11821 cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct
    11881 tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac ttacattgat
    11941 cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc
    12001 agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta
    12061 ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa
    12121 gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat
    12181 tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg
    12241 ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta
    12301 gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa
    12361 gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat
    12421 tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta
    12481 agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt
    12541 gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga
    12601 gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt
    12661 cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca
    12721 ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt
    12781 aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat
    12841 ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt
    12901 cccttgataa acataggcat tgtctactag cacacctaac catacacgat tgacagcagt
    12961 aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga
    13021 atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac
    13081 tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc
    13141 ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca
    13201 agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg
    13261 aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt
    13321 acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc
    13381 aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa
    13441 tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa
    13501 ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag
    13561 gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt
    13621 ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg
    13681 acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc
    13741 ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg
    13801 actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc
    13861 ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta
    13921 atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc
    13981 atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt
    14041 tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag
    14101 tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt
    14161 ccttctt
//
LOCUS       DAHUWM010000062        14009 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHUWM010000062 DAHUWM010000000
VERSION     DAHUWM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.062"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(141..836)
                     /locus_tag="NK788_001276"
     CDS             complement(141..836)
                     /locus_tag="NK788_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCH6769615.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            990..1268
                     /locus_tag="NK788_001277"
     CDS             990..1268
                     /locus_tag="NK788_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769616.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1287..2495
                     /locus_tag="NK788_001278"
     CDS             1287..2495
                     /locus_tag="NK788_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6769617.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2643..3008)
                     /locus_tag="NK788_001279"
     CDS             complement(2643..3008)
                     /locus_tag="NK788_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCH6769618.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3155..3928)
                     /locus_tag="NK788_001280"
     CDS             complement(3155..3928)
                     /locus_tag="NK788_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6769619.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4102..4962)
                     /locus_tag="NK788_001281"
     CDS             complement(4102..4962)
                     /locus_tag="NK788_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCH6769620.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4962..6380)
                     /locus_tag="NK788_001282"
     CDS             complement(4962..6380)
                     /locus_tag="NK788_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6769621.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6437..6886)
                     /locus_tag="NK788_001283"
     CDS             complement(6437..6886)
                     /locus_tag="NK788_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6769622.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7331..7594)
                     /locus_tag="NK788_001284"
     CDS             complement(7331..7594)
                     /locus_tag="NK788_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCH6769623.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7693..8988)
                     /gene="rho"
                     /locus_tag="NK788_001285"
     CDS             complement(7693..8988)
                     /gene="rho"
                     /locus_tag="NK788_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCH6769624.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8985..10256)
                     /locus_tag="NK788_001286"
     CDS             complement(8985..10256)
                     /locus_tag="NK788_001286"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCH6769625.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10421..11290)
                     /locus_tag="NK788_001287"
     CDS             complement(10421..11290)
                     /locus_tag="NK788_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCH6769626.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11582..13192)
                     /locus_tag="NK788_001288"
     CDS             complement(11582..13192)
                     /locus_tag="NK788_001288"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCH6769627.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3987 a         2954 c         2390 g         4678 t
ORIGIN      
        1 ctcacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
       61 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggtc aaaacgctga
      121 tttcacaacc tctttgttca tcaaaagata atattgtctt ctaacaagtc catattcaaa
      181 ataacgatat gatggtcttt aacggtgata ataccatctt ttttcagttg ttttaaaatt
      241 ctgtttacac tgctagcagt tgagatgcca caaaatttgg cgatttcttc attcgtgatc
      301 gaaaaatcga tcagcatccc ttcttctgtc cgcacaccga ataaatcata taattcgtaa
      361 agttgcgtac aaacagctcc cagctttccg tttaatagca tttgctgcat ttttttcatc
      421 gaataaagca gacgatagcg gtaatactct tttacatagt gttgtagctt tgggtcttgg
      481 ttgatatttt gccaaaagcg cacacgattg attcgataaa actctgcata ttctgattca
      541 actcgaatat tatacggcga atcgatgaat tgtgaatatt cgtcttttaa aagagagacg
      601 atttcaagat ctttgatata gcgaaggtta aattctctcc catcatttga aataacactt
      661 gcttttaccg ttccctcttt caagatatac gtatgggaat cttccatccc ttcgaatgtc
      721 aaatatttat gatatccttt gcgtataatc ggaaaatcat ttttatccaa atattccttt
      781 aagactctac tatccaatga tgctccccca tccaaacttt catctaaaaa gatcatacag
      841 tactttaaaa ctttttaaag tccaaaggta ttttactaat gatattaaca tttgttaagt
      901 acctttttcc atcacttatg cgattttttt cctttttttt attcgttata ctgcagaagt
      961 aaaatttttt aagaatagag ggaaaaaaca tgaattactg gatgaacaca atcattaatc
     1021 gattagaaac agcatatcaa acaagatttg atatgaaagc ttctttagtc tttttaaatg
     1081 atgcgtatca aaactcgatc gaattgatca aagcagtgga tgagaatcca acaaacgaat
     1141 gcgaagaatt tttaaatctg tttatgtcta caagagattt atttattcgc caacttgttg
     1201 atcgctatcc atccaattat cacgatgtag aagttcaaat acaaaaatta aaagcatatt
     1261 cagcttaagg ggaatgtata acatacatgg aaaagaaatt atttaataaa tattttattg
     1321 gaatcacatt gatcaatttt attgtctacc tgatttatta tctgttgatg gtcatcatgg
     1381 cagtcattgc gcaagacacc ttacacgcca gcttgagcca agcaggtttt gcttctggga
     1441 tctatatcat cggaacattg ctggcacgct tattcatggg gaaacagtta gaactatttg
     1501 gtcggaagtt taccttaaga ggcggcgcga tcttctatct cctttcaaca ttagcatatc
     1561 tttatacgcc aactatcggg gcattgtatt tagttcgatt cttgaatgga ttcggctacg
     1621 gtacagtttc tacagctaca aatgctatcg ttactgcata tatccctgca tctaaaaaag
     1681 gggaaggcat caattattat ggattaagta ctagtttagc tgccgctgtt gggccatttt
     1741 taggaatgat tttgctgaat atgactgatt ttcgtttcat tatctggttt tcaattgtac
     1801 tggtcttctt tgttacgatt gcctgccttg tctttcctgt aaaaaatatt ccattatctg
     1861 aagaacaaaa aatatctctt cgctcatgga atatcaatag ttttattgaa aagaaagcac
     1921 ttttcattac aggaattgcc tttttgatgg gtctttctta ttcaagtgtt ttatcatttt
     1981 tatcttctta tacaaaagta atccacttag ttgctgctgg gtcatttttc ttcgtcgtat
     2041 atgctttggt catcacattt acacgtccat tgactggaag gattttcgat gtcaaaggag
     2101 aacaatacgt gatgtatcct agctatttat tcttaacagc aggattattt ttactgagtg
     2161 taacgacgaa tagtgtgaca ttgcttatct ctggagcatt agtcggccta ggttatggta
     2221 cgtttatgtc aaatggacag gctgtttgtt taaaaatcgt ccgcgaacat cgaatcagta
     2281 ttgctttatc tacctacttt attggtttag acctaggctt aggcgttggt ccttatttga
     2341 tggggatgct aaaatctctt acttcattta gaggattgta tgtgattgcc ggagtcattc
     2401 cattgatatg tacagcactc tatcttgtta atggccgaaa acatagtcat gaagcagagg
     2461 gcgaattgat tgaatcattg aaaaatgaaa tttgatcttg atgaatagat aaaaaaaacc
     2521 gttcagatct gaaaataatc tgagcggttt tttgagtaaa aatagtttat gattattctt
     2581 aattaaacat ctatttattt ttaaagcttt catttatcga taagaatagt ttaactgttg
     2641 atttattcca ttttccgttc atctggtacg atgccttgac ttagcaacca cttgtggaag
     2701 aaccattctg ccacaccgat caatagagca ggaatcaata ctttcatcca aatatcgact
     2761 gtatcattca taaaatatag atagagccac aagataactg cagctgaacc agcatctgcg
     2821 atcgtcgctg cgatgttccc tactttacgt aagatcaaca gatcccctag cacatagatc
     2881 atgactacaa taattacccc aacaatcgtt gaatccataa agctaacacc gtataaacct
     2941 gtcagcacga cccacagaat cgcccagatc atgattgcct tcactaccaa cgccttcaca
     3001 tgtttcattt tctgcactcc tttctcctgg ataaaaaagt aagattatct aacaagataa
     3061 ccttttcatt attataccat cggctgagag aagcgagcaa agataaggtc ctatctaaaa
     3121 atgaggaagc cacttagaac attcacgtaa taatttatcc gtacagacat tcgatatggt
     3181 gttcctttac tttcgcatac tgttcttttg tcagttcttt acttgtcacc agataatcga
     3241 tttcatgaaa agcaatggct tgatgcatga atttatgtgc aattttttgc tgtgtaacta
     3301 aaacggcaag ccggtctgat tgttttctaa aagctttttt gaccattgcc tctccttcat
     3361 gagtttctgt tactccttgg ctgaaatcta gcccgcgaca actcattatc gctaaatccg
     3421 cataatatga caatgcatcg ttataggctt ttgaaccgtt gattgttgct cgtttcggaa
     3481 caattgcccc tcccaataca gatacttttg ccgttgtata ttcactcaac atagcagctg
     3541 tgacgatgcc attcgtaatg atctgcaaac catcaaatct tgctaggttt ctgactaagt
     3601 gtaaagaggt tgtactggaa tccaagaata agaccatatc atctcgaatc aaagaagaag
     3661 ctgtatctgc aatcttacgt ttttctgttt cttcttctaa tttggtgatc ttcatcaggt
     3721 aaggcacgtc aatttttcga ttgtcaataa gcatggcgcc accgcgaact ctacggatca
     3781 atccactttt ttccagaaaa acaaggtcac gtttaatcgt ggcttcgcta taatttacta
     3841 actcgatcaa atcggtgact cttgcaaatt ggttgttttt tagataattc acaatctcct
     3901 gctgtctatt ctccacttca ttcaacataa aatcacccca tttactttat atagtaacag
     3961 gaaaaaagag gttgtgacag aagtgattgg cttcgagaaa taagaagaaa tttttcgaaa
     4021 aaactacttc tgaacatcgt gtgtggttaa gagggtatga caagactatt gtcataccct
     4081 cttgaccaca catttttatt tttagtataa actagccttg cctactgtgt tcaaaatttc
     4141 cattttatgt ttgaccactt cttgtacctt tttattagct gctggataaa ttgcattcgg
     4201 ctcgtacatc gatggatttt cactcaatat ttctctcaaa gcattgaaaa agacgctttt
     4261 aagatctgag ctcaaattca ctttggcaac tccgtatttt actgcttcgc tgacttctgc
     4321 gtccggatta cctgaaccgc catgtaaaac aaatggaaga tcgatttgtt ggttcaattc
     4381 tttcagtaag tcgatattta attttggagt tctgtttttc ggatacaagc catgcgctgt
     4441 tccaattgct acagcaagtg tatcgattcc tgtttctttc acaaaaatct tcgcttgatc
     4501 aggatctgta taaatgattg tttcagcacc tccttcagca gaaccattat tcccaatcgt
     4561 tccaagctca gcctccactg agacatttac cgcatgagct aacgcaacca cttttttcgt
     4621 aatctgtaca ttgtcgtcga atgatttcaa agacgcatcg atcatcacgg aagaatagcc
     4681 gtttcgaatc gcccgtaaga catcttctat cgtaccgcca tgatccaagt ggataacgat
     4741 cggcagttta cttgtataag cataggctct tactgccgca acaaactcat cacttaaata
     4801 ggcgatttca tcaggatgaa tttctaagat tactggtgct tctaatctct ctgcttcttc
     4861 gatgattgct ttcatcatat catagctgca aatattgaag gctggtactg caaattgatt
     4921 ctctttagcc actttcaata actctttcat tgtatataac attacgcttc cacctcattt
     4981 gttagatttt catttttcgt ccacttgtct tgttttttct ctttcactgt ttttttcaat
     5041 agtccaagca taaaggctgt agtgactgta cctgatgcaa ttgccaagac atacataggt
     5101 aaattgttca taacatttgg aattagcatc acccaaatcc ctccatgagg tgccattgaa
     5161 gtaactccta ctgccatgga aatccctgca gcgactgctg aacctgtcat caaactaggt
     5221 atcacgcgga tcgggtcaga taccgcaaac ggtatagcac cttctgtaat atacgaagcg
     5281 cctaaaatcc agcatgattg acctgaagtc cgttcttctt tggtaaattt atgtttgaat
     5341 aataaagtgg ctaaagcaat acctagggga ggtgtcattc ctgcagccat acaagcagca
     5401 atcggtgcat ttactccagc tgacatcaaa ccgatcgaga aagtcgaagt cgatttattg
     5461 atcggcccac ccatatcagc tgccatcatg atcccgataa gtaatccgaa caatagcccg
     5521 gatgtatttc ccaagctatt cagccaattt gtcaaagcat ccaacaccca tgtgatcggt
     5581 tgttcaatca caaataccat caaaagccca ataattaaga tcgataaaaa ggggacgaca
     5641 atcagctgat aaatagcaga aaattctttt tttacgctaa ttttatttgc cagaaaatca
     5701 cagatatacc ctgctagcac gccaccaatc attccgccaa gaaaacctga accacctgta
     5761 gaggccagta atccggcaac aattcctggg gcaaaacctg cttttccagc aatcgatgta
     5821 gcaatccccg cgcctaacgc aggaaccatc atggcaaacg ctgtatctcc tccgatcctt
     5881 gaaaatgcag ctgctaacac attatagtct gctgagttgg gatcagccgc tgtaatccca
     5941 aaggcaaaac tcagtgcgat gatgattccg cctgcgatca caaatggaag catgtaattc
     6001 acaccattca tgaaatgatt atagattgtc ggctttttct catttgcagc tggttctttt
     6061 tcattagaca caacgaatac gccaatatct ctcaatgctt cgtctattac tacttgcggt
     6121 tctttgattg ctcggcttgt agagacacgc tttacttttt tcccggcaaa tcggtccaat
     6181 tcgatttctt tatctactgc aagaatgatt gcatctgcct ctttgatttc ttgttcagat
     6241 aactgatttt ctgtttttac accttgtgtt tcgaattttg cttgatggtt tagatttctt
     6301 gctgttactt ctaatttttc tgccgacata taagtatgag caattccagt cgggcaagca
     6361 gttacaccaa cgattttcat gattattttc ctcctttttc ttcactatcg atactcggac
     6421 aaagactcta ctattttcat tcttgactaa tctgtctgat gatatctgct ggcgtagtgg
     6481 cttcttttat tccctttcta aaatctgcat tcatcaatcg tctggagagt gtgctgagaa
     6541 tctctagatg catatctgct gatttttcag gaactacgat ggcaaaaata tattcaattg
     6601 gcatttcatc tatcccgcta tagtccgtta atttggctgt atgaaaaaaa cacagtgctg
     6661 gttttgtcac atatttgctt tttgcatggg gaatcccaaa tccatcttct attcctgtgg
     6721 agatctcctc ctctcgtttg aatagtgcct taacgacttt ttgaacattt cttaccttat
     6781 ttgttttttt caacacatcc cctattcgct caaataatga tccttttgaa tctatcgatg
     6841 gatcgaaaac aatcagttct tctgcgatca attgatcaaa cctcataaaa aacctctccc
     6901 ccacaaaaaa tgttaacgtt ttcttggaac tgaggtcagt atagcatcgc tagctgtacc
     6961 gttcaatatt atgaatgaac agaggaaaaa gacccatcat ccgcgtgtat aagtcattaa
     7021 aatgaacgat ctagctcaca aacaatcgca agtctataac aaaacaaaaa gccttccttg
     7081 aaaggaaagc ttagcttcta attttaggta aatggttttt tacttcgaaa taacccctct
     7141 tcatttcgtt aatataaggt gtttttatca agtggataat taaaactata tactagtttt
     7201 tcattgtttt gtgcattatg atcctacgac tttttcagca tcaaacgaag ttttaaattg
     7261 gcaaaaaaca taaaaaaaca gactatccct atcagatagt ctgctaaacg ttgagttatc
     7321 agcgttttta ttattccgca gcgtttgcgt ctttgagacc gtattttttg ttgaaacggt
     7381 ccacacgtcc gtctgcttgt gtgaattttt gacgtccagt gtagaatgga tgtgagtcag
     7441 aagttacttc gacacggatg actgggtatg tattaccgtc ttcccattca actgtttctt
     7501 gtgaagtttt tgttgaacct gacaagaatt tgaaaccagt tgttgaatcc atgaatacta
     7561 ctgggtggta atctggatgg atgttttctt tcatgatttt ttcgctcctt tgccctgctc
     7621 catttgctga accagagttg atttgtcgat ttacaacatt aacatcttaa catgtttcag
     7681 tcaaggttgc aattatcttt tcagatttct ggtcggattt tttttaccaa aggagacatc
     7741 ttgaaattct ttgaaaaatt gctgattgtt ttttgtttta cgcaaaaagc ggatcagttg
     7801 ttccgtatat tctaatgaat ctccagtcat attttttcga agtttccaaa tttcttctaa
     7861 ttcttcactt ggcatcaata attcttcttt tcgggtacct gattttttga tatcgatggc
     7921 tgggaagatc cgacgttcag ataattcacg tgaaagttgt aattctagat ttccagttcc
     7981 tttgaattct tcatagatca cgtcatccat acggcttcct gtatcgacta aagcggtcgc
     8041 taaaatcgtc aaactgccac cttcttcgat gtttcttgct gccccgaaaa atttcttagg
     8101 tttatacaat gcggctgggt caatcccgcc actcaatgta cgtccgcttg gtggaacgac
     8161 taagttatag gcccgagcaa gtctagtaat actatccatc aagataacga catctcgttt
     8221 atcttcgact aaacgcattg cgcgttccaa tactaattca gaaactcgag tatggttttg
     8281 cggctgcaga tcaaatgtag aagataccac gtcgcctttc acgcttcgtt ctagatccgt
     8341 tacttcttcc ggtcgttcat caatcagtag aacgattaat tctacttccg gatagttttc
     8401 ggtgatacca tttgcgattt cttttaatac agaagttttc cctgcttttg gcggtgcgac
     8461 gatcaatcca cgctgaccaa aaccgattgg agaaaagata tcgatcattc gggtagatag
     8521 tctttgcggt gttgtttcca gttgaagctg tctttgcgga taaagaggtg ttaaggctgg
     8581 aaaatgcgga cgttcttttg cttcttccgg atcttttcca ttaacgcttt ccacgtgcat
     8641 caaaccataa taacgttccg attctttagg tggacgggct tttcctgcca ctttgtcccc
     8701 atttctcaaa ctgaatcgtc tgatttgtga ggcagaaata tagatatctt ctgcacttgg
     8761 tccataattg attggccgta aaaaaccgta accatcttga ccaacgatat ccaatattcc
     8821 ttccatataa taaaatcctt gtttctctgc ttgtgcacga atcacagcta gagataattc
     8881 ctttttattc atcttactgt aataaggaat tttaaattct ttggcataac tataaacttc
     8941 ttttaacgtt ttgttctcta gttctgccat tgtcaaataa tcgctcattc agctcccact
     9001 tcctctatca tttcaattga tgcccctaag gctgttagtt tctcgataat atgatcataa
     9061 ccgcgtaaga tataatctac attgtaaatc gtcgttgtcc cttctgccaa tagtccagca
     9121 atcactaaac aagcacctgc ccgaagatca gaagccacga cttctgtgcc gtgaagttta
     9181 tttggaccat tcacgatgat catatttcct tccacggtaa tatcagcgcc cattcgtgct
     9241 agttccggca catggttcac acgtttcgca tagatcgtat cgatgatttc tgaagtgcct
     9301 gtagtcatca atagtaacgc agtaagtggc tgctgcagat ctgtggcaaa tcctggataa
     9361 ggataggtca tgatgtttgc gggttttaat tctttcgagg ggtaaacttc gatttcatct
     9421 tcgctgattt tcatatggac accaatttcc tgcaattttg ctataaagct ttccaaatgt
     9481 tcaaagatca catttttcac tttgatacca tttcctactg cagctgctaa tgctagatag
     9541 gttcctgctt cgatgcggtc aggaatcatg aaatgccgac agccatgtaa tgtttcgact
     9601 ccttcgatac ggatcacatc tgtacccgct cctcgaactt ttgcccccat attattcagt
     9661 aaagttgcca catctatgat ttccggttct cgtgcagcgt tttcaattac tgtctgtcct
     9721 tttgctttta cagcagctaa catcacattg atcgttgctc cgactgaaac cacgtccatg
     9781 aaaatccgat tgccacgcaa tcctttattt tctgtccgta aatacatggc accatgttca
     9841 tttgtcactt cagcacctag agcttcaaat cctttgacgt gtaggtcaat cggtctaggg
     9901 cctaaataac agccacctgg caggcctact accgcttcgc caaatttacc aagcagtgtt
     9961 cccataaaat aataagaagc acgcaaacta ttgatttttc cgtgcggcat cggaatcgat
    10021 acgacttctc gaggatcgat ttctagtatg ttgttggaaa aatggacagt tgcgcccatg
    10081 atctctaaaa tttcaattaa tgaatgaaca tcttgtatgt ccggcacacc ttccaagatc
    10141 actggggaat cagctaagat tgcagctgga atcaatgcga ctgcactatt ttttgcaccg
    10201 ctgatcgtta cttcaccagc taaaggtcgg tttcctttga ttacgatttt tttcatctgt
    10261 ttccaccttg tttattaatc ttttttcatg gatatgcttg tattttatca tagataaaaa
    10321 aaagaaacga gcatggtctg ccgttttaag taaaaaagaa tggagagcga cttcaaaagt
    10381 cgttctccat tcctatatat cgtatctgtt atgtggatgc ttacgctttg ttagctgaac
    10441 cgaaccagtc gatatgagat ttagcatctt caacgattgc tgctttacct ggagctaata
    10501 atttacgagg gtcgaatcct ttaccttctt tatctttacc agcttcgata tatttacgag
    10561 tagctgcagc aaatgataat tggaattcag tgttaacatt tacttttgag atacccattg
    10621 agatagcttt ttcgatttga tcttgaggaa tacctgatcc gccatgtaat actaaaggca
    10681 tgtctggacc tacagcatca gcaattgctt gtaagtggtc aaaagctaat cctttccagt
    10741 tttctggata ttgaccatgg atgttaccga taccgcaagc taagtagtcg atacctgtag
    10801 caaccatttg tttgcattct tcaatatcag ctaattcgcc gttaccgatg attccgtctt
    10861 cttctccgcc gattgaacca acttcacatt caactgagat accttttgca tgtgcttttt
    10921 caacaacgtc tttagctaat ttcaagtttt cttcaaatgg tagatgtgaa ccatcgaaca
    10981 ttactgaagt atagcctaat tcgatacatt ccaaagcagc ttcgtaatca ccatggtcta
    11041 agtggatagc aactggaaca gtgatgttca ttgcttcaac taaatcagtg attaaatctt
    11101 tacagatttt gtaaccaccc atgtatttag ctgcacccat tgatgtttgg ataagtactg
    11161 gtgcttgttt tgcttcagcc gcttcaagga ttgcttgagt ccattccaag ttgtttgtgt
    11221 tgaatccgcc gacagcgtaa ccgcctttac gagcagcttt taaaaattca gctcctgata
    11281 ctactggcat aataaaattc ctcctaagtt ttgtaaaact aaatttgtta agacaattct
    11341 taaccaacga ccctatttta ccaaacattt cggtagtttt ctactaaaat caatgaaaat
    11401 gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc aatagcgctt taatcgaaaa
    11461 aagtcttttg ctttctatct tttttcctac aaataataac aaatcagaaa aagagaaaag
    11521 tgacaagaaa attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa
    11581 attattttgt ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg
    11641 ggcgatttgg acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct
    11701 ctggcagctc tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc
    11761 cttgttcttc aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt
    11821 cttgcacaac atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac
    11881 atgggtataa tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca
    11941 ttaaatcaat aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta
    12001 atccaacgac gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta
    12061 aaaacggaac gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc
    12121 gatcaccaaa tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat
    12181 tttctctcgt taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga
    12241 aaccagaatg tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt
    12301 taccgacaag cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt
    12361 tccattctgt catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat
    12421 ccattccttg tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact
    12481 cgataactgc ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag
    12541 aaacaggttg ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct
    12601 cttttacact gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa
    12661 tcaatgttgt atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa
    12721 gtgcttcaag gaatggcaaa gattctatat cccctactgt tccccctact tctgtaatga
    12781 tcacatctga atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa
    12841 tatgaggaat aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa
    12901 cttcagaata gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa
    12961 aacgttcgta gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt
    13021 ctccgtgctg atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt
    13081 ggatagttac ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc
    13141 cttttccgat tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa
    13201 aaactccttt ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc
    13261 ttcaataaga atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca
    13321 aggtgtataa tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt
    13381 acaatttttt ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca
    13441 aaaaaaattt aacccacata ataagcttga tataaacata gctaacgtta aacctaagca
    13501 aatcagaaaa tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa
    13561 cattatgata gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag
    13621 tttatatagc ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctttacgt
    13681 aaaaaacgta gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa
    13741 aaacaaaaca attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg
    13801 tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt
    13861 cgtgcctcct tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
    13921 aatatccatc aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat
    13981 ggtaggctca caacctctgg ataaacggt
//
LOCUS       DAHUWM010000063        13994 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHUWM010000063 DAHUWM010000000
VERSION     DAHUWM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.063"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(6..335)
                     /locus_tag="NK788_001289"
     CDS             complement(6..335)
                     /locus_tag="NK788_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCH6769628.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="NK788_001290"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="NK788_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCH6769629.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="NK788_001291"
     CDS             1256..2269
                     /locus_tag="NK788_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6769630.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="NK788_001292"
     CDS             2269..3546
                     /locus_tag="NK788_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCH6769631.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="NK788_001293"
     CDS             3609..6311
                     /locus_tag="NK788_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6769632.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="NK788_001294"
     CDS             6463..6780
                     /locus_tag="NK788_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6769633.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="NK788_001295"
     CDS             6810..7130
                     /locus_tag="NK788_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCH6769634.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="NK788_001296"
     CDS             7238..8698
                     /locus_tag="NK788_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6769635.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="NK788_001297"
     CDS             8766..8987
                     /locus_tag="NK788_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769636.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="NK788_001298"
     CDS             9018..9200
                     /locus_tag="NK788_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCH6769637.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="NK788_001299"
     CDS             9200..9613
                     /locus_tag="NK788_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCH6769638.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="NK788_001300"
     CDS             9736..10917
                     /locus_tag="NK788_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769639.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10988..11152)
                     /locus_tag="NK788_001301"
     CDS             complement(10988..11152)
                     /locus_tag="NK788_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769640.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11306..11379
                     /locus_tag="NK788_001302"
     tRNA            11306..11379
                     /locus_tag="NK788_001302"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
     gene            complement(11448..12587)
                     /locus_tag="NK788_001303"
     CDS             complement(11448..12587)
                     /locus_tag="NK788_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6769641.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(12701..13285)
                     /locus_tag="NK788_001304"
     CDS             complement(12701..13285)
                     /locus_tag="NK788_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCH6769642.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(13412..13843)
                     /locus_tag="NK788_001305"
     CDS             complement(13412..13843)
                     /locus_tag="NK788_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCH6769643.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(13861..>13994)
                     /locus_tag="NK788_001306"
     CDS             complement(13861..>13994)
                     /locus_tag="NK788_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6769644.1"
                     /translation="ERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKALEENE"
BASE COUNT         4494 a         2383 c         2794 g         4323 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagag ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt acaaataacc cctgtatcct
    11401 ttgtgataca ggggtttgtt ttgtgtttgt gccaaaaatg tgccaaacta gttatcattt
    11461 ttatataaac tatcgaacac agcattgatt ttttctttat cctgatcttc aagctctcga
    11521 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    11581 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
    11641 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
    11701 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
    11761 ttaacttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
    11821 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
    11881 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
    11941 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtagcacc agcaactaac
    12001 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
    12061 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
    12121 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
    12181 gatgctctaa tatggttatt aaacttaata acggtggatt ttacatggtc tttcgcatac
    12241 ttatttaaaa agcgttgata tccaactcta tctaaatcag aaatcaatac cgctggcata
    12301 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
    12361 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
    12421 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttcg
    12481 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
    12541 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatctact
    12601 ctctttctct gatacaatag gcactataaa gaagcctact gtataggttt gtttttctta
    12661 gaacacgctt gctttggacg gtgggcgtgt tttttattta ttatacagtt gctttaaaaa
    12721 tagctgcttt ttcaaatgat actaaaggag agaagtggat ttctatttct cccttttggt
    12781 ttaacgtgaa atgagcagtt acatccattt gttttcctgg agcaacagat cccattgtat
    12841 tttcgttggc atatgtctct gattttttgt catctggtcc atatacttcc acatctgtac
    12901 ctacagggat atctgaatcg ccatcatttt ttacggtata agtaattttt actacttgtg
    12961 caggttggtt ttcttcaaat tgatttcttt catcagttag ttctacacta tttagtgtat
    13021 attcagcatc accaactttt acagtgtcac caatcttata gaaggtatcg cttttttctt
    13081 ctttagaaga tgaagcagag gttgaagatt tagttacttt ttcgccacca ttatcacttg
    13141 ctttattact atctgatcca ccatttaaag cagaaccaat aattataatt aaaattactg
    13201 ctaataccca aaaccatact tttttgtaaa aaggtttact aactttatac atttttccgt
    13261 cttgacccat aacttttttt gccatttaaa tattcctcac ttcttgttat aatatatttg
    13321 cgatctcaga aatgaggtat gagtccgtgt gccagcacgg gcttttttaa tgttttgctg
    13381 aaatcggttt ttttaataat tcttgatgta ttcatacata ttgccttgcg taagaatatt
    13441 ttttcttaaa atagcattgg cagacagcat aacaagtgta tctgagctta ttctgaatag
    13501 aataatattc cataaatttt tcgaggttaa actgagattc atcagtcagt tcgttctcaa
    13561 tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca aattttgagt
    13621 gaaaagttgt agaagtatcg tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg
    13681 caagttcatg agataaatga aaggcttctg cagtttcgcc atatagattt tcattcaaaa
    13741 aaatgattct aggcttcgga taatagaagc caggctcttt catttccatg taaactactt
    13801 tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaccc atcactccaa
    13861 ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg tctccttaga
    13921 tatttctttt ccatcaaaag aaaaaacagt atcgttttct gataaatcca catgtttagg
    13981 ggtttctctt tctt
//
LOCUS       DAHUWM010000064        13789 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHUWM010000064 DAHUWM010000000
VERSION     DAHUWM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.064"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(15..875)
                     /locus_tag="NK788_001307"
     CDS             complement(15..875)
                     /locus_tag="NK788_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCH6769645.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            967..1395
                     /locus_tag="NK788_001308"
     CDS             967..1395
                     /locus_tag="NK788_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCH6769646.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1557..1733
                     /locus_tag="NK788_001309"
     CDS             1557..1733
                     /locus_tag="NK788_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCH6769647.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            1760..2206
                     /locus_tag="NK788_001310"
     CDS             1760..2206
                     /locus_tag="NK788_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCH6769648.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2360..3739)
                     /gene="brnQ"
                     /locus_tag="NK788_001311"
     CDS             complement(2360..3739)
                     /gene="brnQ"
                     /locus_tag="NK788_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCH6769649.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            4445..5416
                     /locus_tag="NK788_001312"
     CDS             4445..5416
                     /locus_tag="NK788_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCH6769650.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5440..7632
                     /locus_tag="NK788_001313"
     CDS             5440..7632
                     /locus_tag="NK788_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCH6769651.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            7649..8125
                     /gene="ybeY"
                     /locus_tag="NK788_001314"
     CDS             7649..8125
                     /gene="ybeY"
                     /locus_tag="NK788_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCH6769652.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            8109..8504
                     /locus_tag="NK788_001315"
     CDS             8109..8504
                     /locus_tag="NK788_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCH6769653.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            8519..9418
                     /gene="era"
                     /locus_tag="NK788_001316"
     CDS             8519..9418
                     /gene="era"
                     /locus_tag="NK788_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCH6769654.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            9561..10373
                     /gene="recO"
                     /locus_tag="NK788_001317"
     CDS             9561..10373
                     /gene="recO"
                     /locus_tag="NK788_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCH6769655.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            10757..11674
                     /gene="glyQ"
                     /locus_tag="NK788_001318"
     CDS             10757..11674
                     /gene="glyQ"
                     /locus_tag="NK788_001318"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCH6769656.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            11676..13751
                     /locus_tag="NK788_001319"
     CDS             11676..13751
                     /locus_tag="NK788_001319"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCH6769657.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         4644 a         2478 c         2891 g         3776 t
ORIGIN      
        1 aaagacaaaa atgattaatc gcttttttct gttgtttcag ttccataata actatgatct
       61 tctaggtcta aagcattcaa aataagcgaa gccgtttctt caatagataa agatgccaca
      121 ttgatcacta cacatcctag tttttcataa agatcattcg caaaatcgag ttcagctcta
      181 attttttcga tatcggaata agcagtatca gggtttaatc cgtaagagcg catccgttct
      241 ttacggatat tatttaaaat atctgggtta ttcgttagtc ccacgatttt tttaggatcg
      301 atttcccaca gctgtttggg aatatgtgct tggggaatca aaggtaaatt agcaactttt
      361 aaatttttat ttgctaaaaa caagctcaaa ggcgttttgg aagttcggga tacgcctagc
      421 aaaacgacat ctgcttctaa aaaaccacga ggatctttcc catcatcgta ttttacagca
      481 aattccattg ctttgatccg tttgaaatag ttctcattca ataaatggag ggcaccaggt
      541 tgccgaattg gcttgatacc agttaaacgt tcaatttctg atatcggtgg cgtcatcaca
      601 tcgaaactaa ataattgatg ttcttcacaa aaagaacgtg ttatctcgat cagttcttga
      661 ttgattaatg tatgcaggac aagtgctttt tcttctaatg catcttgcaa tgcctgcctt
      721 aatgtttttt catctgtcac gaacgttctg cggaataggt tgaactcaac ggtaggatac
      781 tgagccatag tagcttgcgc tagttttgtc gcagtttctc cagcagagtc ggagatgaca
      841 aaaaaagtaa cgatttctgt agaatcggaa gacatggata aaacctcact tgcatttttt
      901 ttatgtttat tataccatac tctagaatgc gtacttcgta atcattttga tttggaggaa
      961 taagtcatga aaaaacagac attgatcgaa caatcattag aaacattgaa aaataacggt
     1021 tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg taaccgctac
     1081 gtatctgcta aagaagtttt tgactatatg aatcaggaat tcaaaggggt aagttatgat
     1141 acgatttacc ggaatctcca tgattttgca gagatcgatt tactggaaga aaccgattta
     1201 aatggtgaaa tgaagtttcg ttttcattgc tgtcaggggg aaatcggcca tcaccatcat
     1261 tttatctgta cagtgtgcgg aacaacgaaa gaaatccata tgtgtccaat gaattatttc
     1321 gaagaacagc taggtggatg cacgatcgaa gggcatagat tcgagatttt cggaagatgt
     1381 gaaaattgtc aataaggatt taaataaaat aacatttgat aatttaaaaa aaaagcaaga
     1441 tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct tgttttttga
     1501 gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga attaacatgt
     1561 caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc ttcaaacgtt
     1621 ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac gaaaaaccaa
     1681 gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc tagttctgtg
     1741 atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca tgaaagcagc
     1801 gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt catctataca
     1861 aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaactaa cggtgttgtc
     1921 tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag gacgtgacga
     1981 tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa aaatatatgc cggaacaact
     2041 ttccgacgaa gaaattcgac aacttgttca agaagcaatc actcaaactg gtgcttcatc
     2101 aatgaaagaa tttggcaaag ttatgggtgc aattatgcct aaagtcaaag gaaaagcaga
     2161 tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa tcttaatttt atcatttaaa
     2221 ctgcggacag tgattgtccg cagttttttg tgttcgtaaa aaaagaacta aaaaacagct
     2281 agaagaaaga ataagattct ttcttctagc tgtttttttc ctgtttaata tctcttatgt
     2341 taaattgtgt ttgatttggt tattcttctg gtagttcatt tgcttcttgt ttataccctg
     2401 ataggcgata ggcgtaataa aagagtaagc tgccagcgta accaagtaac gttgggacca
     2461 tccagccaag tcctaatgca gcaaaaggaa cgtgatgatg ataaaatcct agtaagcttg
     2521 ctacgacagt ttgctgactc actaatgcag gcgaatttgc caacatatcc aatacggctg
     2581 gaacggcaga aaagaacatc gttacttgat agacaaatgg actcttttgg aagaattttg
     2641 ctgttaagga aagcaagatt aacgcaagtg acaatggata caaaagcatc aatactggta
     2701 aagaccattg gatgatatta tccaatccgg cattcgcaac gacaaaagag acaaaagtcg
     2761 ttaaccgcag ccatgtcata taactgactt tagggaacag tttatggaaa tcttgtgcaa
     2821 aagaaacaac gagtcccata gcggtagtga agacacctaa tgtgaccatt acccctaaga
     2881 agctagcacc gaagttacct aaataaaaat tagtgatctg agaaagtgca gttccgccat
     2941 tttctgataa aggcatctta ttcaaactca tcgcaccgag aaggaccaga ccaaaataaa
     3001 tcatgacttc taacgcaacg ctcaggctac cagcttttgc tgttaattct gtgacttttt
     3061 tatctttaaa accaagatca cgaactgcat ggacaaaagt gacacttaaa gcaagtaaag
     3121 ctactgcatc aaccgtgttg tatccttcta aaacgccatt tgtcaaagcg ttcgtctgat
     3181 aagaatctgc agcagattgg tttagattcc ccatgggttg gaataaagaa acgacaaaaa
     3241 tgatagctaa taatgcaaga aacaaggagt ttaaatactt tccgacatat ttgaccaaac
     3301 gtgttggatg tacagtaagg aaataagcaa gtaaaaagaa aagagcagag aataaaaaca
     3361 tacctaatga atcatatttt tcaggcaaaa agggtttagc agccatttca aaagcagttg
     3421 ctgctgtccg cggtgtacca aagaatggtc cgatcgttaa atgaacaagg atcaagaaca
     3481 aggcagcata ccattttccg acaggtttag ctaagtcata cagaccatca ctttttgtca
     3541 ctactacagc taagatagct aataatggga aaagagcgcc tgagatggca aatcctacac
     3601 cagcgctgat ccagtttgat ccagccattt gacctaagtg gacaggaaag atcagattac
     3661 ctgacccgaa gaacatcccg aagataagtg aactgaccat caataattgc ttccatgaat
     3721 aagtttttgt ttcttccata aatttttcct ccaataatta tcattttgag taacaaaata
     3781 aacgttgcag aaactagaaa tgccactaat atcagattcc atgaaaacca ccaccttaaa
     3841 atataaaaaa aagccctttt ccacttggga aaagggctct agattctaga acggtaccac
     3901 cttttttcgc ttaaaaaagc ttcttcattc acggacgctt ttcgataaag ttatccgcta
     3961 gcctgataat ggaggcagcc acaatgatct acgttttcga cattgactcg gaaatgatgt
     4021 tcgtctattc gatttattgt catttctcac ttacatgact cactgggaat aaatcgctga
     4081 atagcctact gttttcctcg acgctttgct gtatatttat ttgttgacgt tataatacac
     4141 ctgttttttt tgaaatgcaa gaatatttca aatattttgt tttctttttt tgaaacaaga
     4201 aaaataccca gagaatactt gctaagttag acggaatact tgtcctctat cttatgcata
     4261 gcttacatga tcagcttttt ttattcaaac gagaccttta gagaatggaa gcagctattt
     4321 tttaacgttt cttggtactt atggttaagc aactctttta gaaccttttc ttatttgcta
     4381 tatagtttct tctgagtatg gtaaaatagg aaagaatgaa tcgaaaaaaa ggggatgtct
     4441 atttttgacg gaagagtcta gttctttaga gattagattg acggctgctg atgatgtcag
     4501 catgctttta ggtgcacacg acaagcatat aaaactgatt gaagaaacaa cagaaacaac
     4561 catccataca cgtggagaaa tcattcagat cattggcgaa caatcccaga tttccttggc
     4621 tgcctcagtc attcggacat tgcaggaatt gatcaaacga ggaatccatg taagtacacc
     4681 agatgtcgtg acggcactga aaatggggca aaaaggcaca ttagattatt ttgttgaaat
     4741 gtatgaggaa gagatcgtca aagaccgtaa tggaaaacca atacgggtaa aaaacagcgg
     4801 ccaaaagaaa tatgtcgaat cggtagccaa acatgatatc gtatttggcg taggaccagc
     4861 cgggacagga aaaacttttc ttgcagtagt attagcaatc gctgccttga aaaaaggtga
     4921 agtgcaaaaa atcattttga cgcggcctgc tgtggaagca ggggaaaatc taggctttct
     4981 tcccggggat ctaaaagaaa aagtagatcc ttatctgcgt cctgtttatg atgctttata
     5041 ccaaattttt ggattagatc atacgaaccg attgatggaa cgaggcgtga tcgaaattgc
     5101 tcctttagct tacatgcggg ggcggacatt ggatgatgca ttcgttattt tggatgaagc
     5161 acagaacacg acagttgctc agatgaaaat gttcctgaca cgtcttggtt atcaatcaaa
     5221 aatgattgtc aatggagata cgagtcagat tgatctgcct cgagggacaa caagtggtct
     5281 tgttcatgca gaacgaacat taaagcaaat caagaaaatc gattttgtta attttgaagc
     5341 aagtgacgtt gttcggcacc ctgtagttgc cgaaatcatc aaagcttatg aaaaggcaga
     5401 tctccatcaa gaataagctt tgcttaggag gataagcaca tgttgaataa aacactgcag
     5461 aaactacaag aaaaacttgg aaaggcagca ttgcctttcc tgctgttctt gttttttatc
     5521 attatctgcg gcgttacatt tagtagtgtc aaacaaaagg ccaatgattt cagagaaggc
     5581 caagtagcag aagaaagtat ccgtgcgaac aaaacgatcg agaacacgga agaaaccgaa
     5641 caaaaaagaa aactggcagc agaagcagtc actccagaat acacttacca aaaagatctt
     5701 gcagatgacc agaacaatcg gatcaagcaa ctatttgaat tgatcgataa aacgaatgat
     5761 gccatcaaca aatcttatga cgaaaagatt gataaggcaa aggaagatga aaatatccca
     5821 aagcctacta cagaagagcg gatcgccgcg ttgaagaaaa attttgaaaa tgtgaatgca
     5881 gaaaatgttg cgttttatca aaaactaccg aacaattttt attcgacgat atttgaaatg
     5941 actgaaactg agatccatac tgtgagggat gagagtctga agctcattga cgaacagatg
     6001 agcaaacaag ttcgtgaaag tgaattggaa gcttttaaac aagaagcaga agagcaaatc
     6061 caatatttga atgtcacacc agcacaacaa caaatgattc gctacttagt ggatcaaggt
     6121 atcgtagtga atgacgtatt gaatgagaaa aagacagaag aattgaaaca atccgcacga
     6181 gaagctgtac aaccagtgat gatcttccaa ggagaaatca ttgttcgtga aggtaatcaa
     6241 atcgatgctt cagctatgaa aaaattggaa ttactaggac tgactaatca aacaacttcg
     6301 atttttccgc taattgccat ggttttagct gttttgcttc aaatcggcgt actgatttat
     6361 aattcacttc aattccaaaa tgcaggagaa agaacaaaat acgtgctttt ctatgtgaca
     6421 gctatgtcag tcagcatagt gctgatgaaa tttttccaat tattccaaac ggaacaagct
     6481 acttatatcc cgctgtttta tcccgctgct tttgcaccac ttgttttaag ctttttcttg
     6541 aaccgaagag cagggatcat ggctggtatt ttccaagcag tatctgcact tttcattttt
     6601 tatgattcga ttggcacgaa ctttctaacg attatcttga tgtcttatgt tttctctggg
     6661 ctcatggcaa cgattgtcag acgcaaacgg atctcagaac aaggattatc tgctgccatg
     6721 tgggtcatta tttttcctgt gatgatggat gtcattttga tcgtctatca aggaatgagc
     6781 tttggtgatg caacgacttg gttaatgctt gtctgcgggt tcactggtgc actcttctca
     6841 ttcttgctga cgatcggtct acacccatat attgaattga tggtcaatga tgacagtgta
     6901 attgtattaa atgaactcag caatccaaat catcctttat tgaaacagtt gctggaagaa
     6961 gcgccaggta cttatcatca tagtatgatg gtagctaatc taagtgctaa cgctgtagca
     7021 gaaatcggcg gacgttcttt gctaactcga gtagcttgct attatcatga tatcggcaag
     7081 atcaaacatg ctagcttttt cgttgagaac ttgccttcag gagcggaaaa cccgcacaat
     7141 ttcttattgc cagaagacag taaacatatt atctttggac atgtgacaga tggagccaaa
     7201 atattggaag attataatat gccgcaaatg gtgatcgata tttgcaaaca gcatcatgga
     7261 accacgctaa tgcgttattt ctacatcaaa gcgaaagaac gcaatccgga agtaacagaa
     7321 gagcaattcc gttatcctgg accaaaaccg caaagtcgtg aagcagggat cgtcaatatt
     7381 gctgacagtt gtgaggctgc agtacgggca atggatcatc cgacgagcga aaaaatcgaa
     7441 agttttgtcc ataatcttat cgaagagcgt atttccgatg gtcaattaga cgatagtgga
     7501 ctgaccttga aagaaatccg aaaagtggaa aaatcattga tcagcggttt aagtagcacc
     7561 ttccattctc ggatcaaata tcctaaaatg aaaacagaag ttgagaagat aaaggaagaa
     7621 caagaagaat agaaagaagc gatgtataat ggatattacc tttatggatg aaacaaacca
     7681 agtctcagaa gagaaaatga aagaaatcga tgacttgctg caatttgcag catcttattt
     7741 aaagctgcca gaggaaacag aaatgtctgt tactttcatg gacaatgcgg caatccaagt
     7801 aatcaatcgt gattaccgtg gaaaagatgc accgacagac gtcatcagtt ttgctctgga
     7861 agaagaaggc gaagacgaaa tcccagtgat ttttgaagat ggagagaatc cattgcctcg
     7921 tgtactagga gacatcatga tttctattga acgtgctgct gaacaagcag aagcttacgg
     7981 ccattcatat gatcgggaac ttggcttttt agcggtacat ggattcttgc atatcaacgg
     8041 gtatgaccat atgacaccag aagatgagaa agaaatgttt ggtttacaga aagagatctt
     8101 ggatgcctat ggactcaaaa gataaaaacg tagagaaaaa caaacatttc ctgatgtctg
     8161 tggaatttgc tgtccaagga gtcaaaaccg tttttgagga agaacgcaat atgaggaaac
     8221 atgttgcgtt cggtatcctt gctttattta tgggcgtgat tttccagctt gatcggatag
     8281 agtggttatg gctgctttta gctgtttttc tcgtttggat agtagagatc atcaatactg
     8341 tttttgaaaa tgtagtggac atgtttacgg attttcattt tcatccgatt gggaaaaaaa
     8401 tcaaagatat ggcagctgga gcagttttag tgaccgcttg ttttgctgtc atcattgggt
     8461 tgattttatt tgtccctaaa atatatcatt tgttgtttaa ttaaaaagga gaacgtatat
     8521 gccagaacat aaatcagggt ttgtcgcaat cgtcggccgc ccgaatgtag gaaaatccac
     8581 attattgaac cgaatcgtgg gtcaaaaaat tgccattatg agtgacaagg cacagacaac
     8641 tagaaataaa atccaaggag tatatactac accagaagct cagctgattt ttatcgatac
     8701 accaggaatc cataaaccaa agcatcgtct aggagatttt atggtagaag cagcttacag
     8761 tgcgttgaaa gaagtggatg cagtcttatt tatgatcagt gctgatcaaa aacgcggccg
     8821 gggagacgat tttattattg aacgtctaaa aaacgtgcat tctccagttt atctgattat
     8881 caataaaatc gataagatcc atccagacga gttgctggga atcatcgaag attattcgac
     8941 gcagatgcct tttgcacaag tgattccgat ctctgctaca gaaggaaaca atgtcgaacg
     9001 cttaatggat gtattagtca gtgaaatgcc agagggccca caatattttc cagatgatca
     9061 agtgactgat catcctgaat actttatcgt ttctgaattg atccgtgaga aagtcttgtt
     9121 tttaacacga gacgaagtgc ctcattctgt cgcagtcgtt gttgattcga tgaagcgaaa
     9181 cgagaatgat aaaatccata tccaggccac gatcatcgtc gaaagagata gccaaaaagg
     9241 aatcattatc ggcaaaggcg gaaaaatgct gaagcaagta ggaacgaaag caagaaagga
     9301 tatcgaaaat cttttaggag acaaagtatt tctagaactt tgggtcaaag tccaaaaaga
     9361 ctggcgagac aaaaaagtct atctgcaaga ctttggctac cgaaaagacg aatactgata
     9421 ttctgaactt cttataggtt aagtatagaa ataaactgga taaaaatccg cacgttgaga
     9481 aaaacggcgg aagatcgaaa cataaataat ctggatcggc agaatctgct tttccagatt
     9541 tattttttag gagggagaat atgagccagc ttgcagaatc gaaaggcttg attttatcta
     9601 gccgtaatta caaagaaaaa gataaattag tcaaaatctt tacggaatct tctggcaaaa
     9661 tgatgtttta tatcaaagga atccatcggc aaaatcagcc attagctccg gctttgctcc
     9721 cctttaccga agcagtctat atcggtaatt ttcgaagtga aggtttgtcg tttttaagca
     9781 gtgtcaaaga cgtccatgcg tttcggaaca tccaagaaga tatctttttg agcggttatg
     9841 gtacctattt gctaaatcta gtagatgcag caatcgaaga tcatcaatat gacccgcatt
     9901 tattccaatt tacgcaacaa gctctgcagc ggatggatca aggagatgat gcagaaatca
     9961 tcacgaatat ctttgaagtt caaatactgc aacgcttcgg aatcgcgcca atttggacgc
    10021 actgtgtcgt atgcggggaa acaaaaggaa aatttgacta ttcttccaaa tctggcggtg
    10081 tgatatgcga gaaacactgg ccaatggacg aacaccgtta tcatgcagat cctcgcgctg
    10141 tttatttcgt tcgaatgttt tcggctattt cttatgataa gatcagtgga atccacttga
    10201 aagaagaaac gaagcaggca atccgtgacc tcattgacca gttgtatgat gaatatgtcg
    10261 gtattcatct aaaaagcaaa aagttcattg atcaaatgaa gtcttgggca cacgtattaa
    10321 agacgccaga gaaaaatatg cctgaaaaag agatacctga aaaagaagaa taaatgaaag
    10381 atacaacaaa aaaatcaaaa ctatttgaca aatacaggga cttccgtcta ttattaaccg
    10441 tagattaaat agatcgttca ctgagaggat ctattatata aacaagaaaa ctgcaaaatg
    10501 aatacgtaca ttgaccaaga tcagtaaaaa acacgtggtt tagcgagttc gggagagtgg
    10561 aagccggacc caaacgattt ttgaagaggc acttgcgagt acgataaatg aagctgccga
    10621 atctttattc ggaagtaggg tggaaccgcg aaattattcg tccctatgtc tgtaatcaga
    10681 catagggact ttttgtcgtt ttctttcctg tattcttatt gacagcaacg cttaaaaatt
    10741 gaaaggggca aaacagatga gtaaaaaatt gaccgtccaa gatatgattt taacattaca
    10801 aaaattttgg tcatcaaacg gctgcatgct gatgcaagct tatgacacag aaaaaggagc
    10861 aggtacgatg agcccatata cattcttacg ggcaatcgga ccggaaccat ggaatgctgc
    10921 ttatgtagag ccatcccgcc gaccagctga cgggcgttat ggagaaaacc caaaccgttt
    10981 gtatcagcat catcaatttc aagtcgtaat gaaaccttca cctgaaaata tccaagagct
    11041 ttatttagaa agcttgcgtc ttttaggaat tgatccatta gaacacgata ttcgttttgt
    11101 tgaagacaac tgggagaacc catcgatggg gtgtgccggt gtgggttggg aagtatggct
    11161 tgatgggatg gagatcacac aattcacgta cttccagcaa gtcggaggac taccttgtaa
    11221 accagttact tctgagatca cttatggact agaacgtctt gcttcatata ttcaagaagt
    11281 tgaaagtgtc tatgatctgg aatgggctga tggtgtgaaa tatggcgaga tcttcaaaca
    11341 accagaatat gaacattcca aatattcttt tgaagtaagt gaccaagact tgctactaag
    11401 caactttgac cgttttgaaa aagaagctaa acgctgtatc gatgaaaatc ttgttcatcc
    11461 agcgtatgat tatattttga aatgcagcca tacattcaat ttattagatg ctcgcggtgc
    11521 agtatccgta acagaacgag caggttatct ttcccgtatc cgcaacatgg ctcgtgctgt
    11581 agccaaaatc tttgtggcag aacgcgaaaa attaggattc cctttattga ataaaaacac
    11641 agcaaatcca gaaaacatga aggaggctga ataagatggc aaaagactta ttacttgaaa
    11701 tcgggctgga agaaatgcct gctcatgttg tcacaccaag catgaaacaa ttagaagaaa
    11761 aaacagcaaa atttttagat gaaaaccaat tgacttatga tacgatcgaa acgttttcta
    11821 caccgcgccg cttagcaatc aaagtaacaa acattccaga acgtcaagca gacagtgaag
    11881 aagaagtcaa aggaccagcc aaaaaaattg ctttggacgc agatggaaac tggacgaaag
    11941 cagctgaagg atttgtccgc ggacaaggtc tgacgacaga tgctatcact tttagagaat
    12001 taaaaggcgt ggaatatgtc tatgtaacga aatacactca cggcaaagaa gcgaaagaag
    12061 tattgacagg tttagcagat gtcattaaaa gcctcacttt cccagtaacg atgcattggg
    12121 cagaatacga ttttgaatat atccgcccta ttcactggat cgtggcattg ttagatgacg
    12181 aagtaatccc gtttgagatc ttagatgtca aaacaggcag agcctctcga ggacatcgtt
    12241 tcttaggcga agatgtgaca tttgcgcatg caaaagaata tgaagaaaaa ttgaatgaac
    12301 aattcgttat tgcttcaccg caaaaaagac aagaaatgat cgttacacaa gcaaatgaat
    12361 tagccgaaaa aaatcactgg gttattgatc tagacgaaga tttactagaa gaagtcaaca
    12421 atttagtcga atatccaact gtcttttctg gcgacttcga agaaaaatat ctttctgttc
    12481 ctgaagaagt actagtcact tctatgaaag aacatcaacg ttatttcgag gtacggaacg
    12541 atcaaggtat gctgcttcct cattttattt ctgctcgtaa tggtgatcgg gtaaaattgg
    12601 aaaatgtggt tcgaggcaat caaaaagtat tgacggctcg tttagaagat gcggaattct
    12661 tttataatga agacaagaaa gcttcaatcg accaatacgt tgaaaaacta aaaactgtta
    12721 ctttccatga aaaaatcggt agtatctatg agaaaatgca gcgggtacaa gtcattgccc
    12781 gtatcattgg cgaacaagta ggactatctg aggaagaatt gatggatctg caacgagctg
    12841 ctgaaatcta taaatttgat ctagtaacca acatggtagg ggaatttcct gaactacaag
    12901 ggatcatggg cgaaaaatat gctctgctga aaggcgagaa acctgctgta gcaacagcga
    12961 ttcgtgagca ttacatgcct atatccagtg aaggagagct acctcagtct acagtaggtt
    13021 ctgttttagc gattgcagat aaattagata gcattttttc tttctttgca gttgggatga
    13081 ttccaagcgg gtcaaacgat ccttatgcct tacgtcgcca agcatacggt gtcgttcgga
    13141 tcatcgaatc gaaaaaatgg gcctttcctt tgtctgttct gcaagaaacg attagtgatg
    13201 ttatcagtaa agatacggat cgtttcggca ttggtttatc agaagggcaa caacaggtga
    13261 tcgactttat caaaggccgt ctgcgccaat tattgacaac gaagaatatt cgtcatgatg
    13321 tcatcgaagc tgttttgaat gcagaacaaa aagatctgac aaaagtattt gcagcagcac
    13381 aattgttcaa gcagcatctt gcagatgaag acttcaaacc atcgatggaa gctttgacac
    13441 gtgtcgtgaa cttagcgaaa aaagcggagc ttgaaaaaca atcagaagta gaccctgaac
    13501 tgttcgaaaa tgaagcggag aaagaactgc acaaagcagt agaggcaatg aaaaaaagct
    13561 ttgctgacca aacgatcaac gaaaattatg atcagcttgt aagccttcgc ccactgatcg
    13621 aaaattattt tgatcagacg atggtcatgg cagaagaacc agcagtacgt aagaaccgat
    13681 taacgcaatt aagtcaaatt gctgccatgg ctttgtcgct agcaagttta gatcaaatca
    13741 tcacaaaata atttctaata tgtaaataaa ccagagaata ggcacaaaa
//
LOCUS       DAHUWM010000065        13756 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHUWM010000065 DAHUWM010000000
VERSION     DAHUWM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.065"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..199)
                     /locus_tag="NK788_001320"
     CDS             complement(<1..199)
                     /locus_tag="NK788_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769658.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWV"
     gene            complement(678..1397)
                     /locus_tag="NK788_001321"
     CDS             complement(678..1397)
                     /locus_tag="NK788_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCH6769659.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1446..3092)
                     /locus_tag="NK788_001322"
     CDS             complement(1446..3092)
                     /locus_tag="NK788_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6769660.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3271..3861
                     /locus_tag="NK788_001323"
     CDS             3271..3861
                     /locus_tag="NK788_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6769661.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3953..5458
                     /locus_tag="NK788_001324"
     CDS             3953..5458
                     /locus_tag="NK788_001324"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCH6769662.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5542..6894)
                     /gene="celB"
                     /locus_tag="NK788_001325"
     CDS             complement(5542..6894)
                     /gene="celB"
                     /locus_tag="NK788_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCH6769663.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6894..7412)
                     /locus_tag="NK788_001326"
     CDS             complement(6894..7412)
                     /locus_tag="NK788_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769664.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7428..7754)
                     /locus_tag="NK788_001327"
     CDS             complement(7428..7754)
                     /locus_tag="NK788_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCH6769665.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7767..9746)
                     /locus_tag="NK788_001328"
     CDS             complement(7767..9746)
                     /locus_tag="NK788_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6769666.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9767..10081)
                     /locus_tag="NK788_001329"
     CDS             complement(9767..10081)
                     /locus_tag="NK788_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCH6769667.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10249..10542
                     /locus_tag="NK788_001330"
     CDS             10249..10542
                     /locus_tag="NK788_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCH6769668.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10620..11732)
                     /locus_tag="NK788_001331"
     CDS             complement(10620..11732)
                     /locus_tag="NK788_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6769669.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12119..12298
                     /locus_tag="NK788_001332"
     CDS             12119..12298
                     /locus_tag="NK788_001332"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769670.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12492..12662
                     /locus_tag="NK788_001333"
     CDS             12492..12662
                     /locus_tag="NK788_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6769671.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13086..>13756)
                     /locus_tag="NK788_001334"
     CDS             complement(13086..>13756)
                     /locus_tag="NK788_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6769672.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVA
                     IFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRK
                     KRNI"
BASE COUNT         4313 a         2798 c         2352 g         4293 t
ORIGIN      
        1 ccacccaagg actttccctc aagctagcca agaacccatc tgctgaaata tgaaaatttt
       61 tgaatgctac gacattcgcc attacaggta tgtaaaagaa aaaaatcatc caaagcatac
      121 ccggtacagc catcaggaca agtgctttat aacgccatag attcataaaa aaatgattga
      181 acccctttcg ttttttcatc taaaaacccc tttcgattca ttcgcaacgt aattatagcg
      241 ctttcaataa tgagaataaa taaacaaagt atagggaaaa tgaaacaaag tataggagag
      301 gttgtgaaat cagcgttttg gcctgagtat tagagaaaaa aacgggaaaa tagttcctca
      361 tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcagag
      421 gttgtgaatt cagcgttttg gcctgagtat tagggagatc gtgtccttac tatctaactc
      481 tgagtcacaa ggaacaatgg ggactgtaac aaaagtcttg tcacagtccc ttattccgaa
      541 caaacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaaagccat
      601 ccgaaagtca gacggacaag tctgcttttc agatggcgct acaactactc tgcttattct
      661 tttagctatc cattcaatta tttttctctc acttgttcta gctcttcttt ggtcatgggt
      721 cgatattgcc ctttattcaa atctttaggc agccaaaatt ttcccatacg gattcttttt
      781 aaatataaga cttcttttcc tactgctttg accattcgct taacttggtg aaactttcct
      841 tcatgcaaaa tcagacggat aattgaagtt tctgtctctt gatctacttg atcaatttgg
      901 agttttgccg gcaatgtctg ctcatcgcca tcaataatca gtccttctgc aaataaacgg
      961 atatcttctt ctgtcattat accgctaacc tcagctagat attctttctc cacatgtttt
     1021 ttaggtgata gtaactgatg ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct
     1081 gtgtctttat ctaatcggcc gactggaaaa agatcttccc gataatcctg gtcttccaat
     1141 aaatcgatta cggtttgatc atgattatcc tgagtagcag aaatgactcc tgcaggttta
     1201 tgcaaaatgt agtaaaaatc tttttgatac gtaatgattt ctcctaaata agtaaccgca
     1261 tcttcgaatt catttacttg cgctttatcg cttttcacca cttgtccatt gacttcgatc
     1321 gttccttttt tgattaatcc tttgacttct ttgcggctgc caagtcccat atctgctaaa
     1381 aacttatcta aacgcatgcg tttcccccat ttcaaaagag catcaatcta gctcttctga
     1441 ttcacttatt ttatttttaa tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt
     1501 tctgccaagc ggattttcaa agctaaataa ccatacacta aagcccctat acctgctact
     1561 acaagaatca acaatagaga ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc
     1621 atctttgtga tccacgaagc caaaagcata atcaaggtaa gaataaagat cagcaatgtc
     1681 ctgcgccaga caaaacttgg tttaaagcgg gctacttcat ggattctctt taagatcaag
     1741 gtacaagaca cagcaaatcc gatcattgtc gccaataaag ggccgtatac ttcaaataaa
     1801 cgaatggctg ggtattgaag aaggagtttc actagaaacc caataaccaa ataattaatt
     1861 gccgccttgt tttcaaacat tccttgcagc atgttggaaa ctagcatata tagtcctagg
     1921 aagagtccta caaaacttgc ttggatcagt acattacttc ctagagaatc cggtgtataa
     1981 aataatgtat ttagcggata ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa
     2041 aatgaaaaca actgtaaatt tccgcttgtc aattttgcca gacctactcg atctttcaac
     2101 gtaacagctt ctgtaatcaa cggtaaccca gtagacgcga tcgatgttgc caatgcaata
     2161 actaccatcg tcaacttatc tggattggca ctaaaaatcg aaaaaagatc cagtaactgt
     2221 gcattggaat actcggtgga tgcagacatg atcttcataa aagtgaactg atcgactaat
     2281 ttgaagatcg ttacccctga acccacaata ataaacggaa tcgcttcttt aacggtatct
     2341 aacaacaatt ccttcgttgc aatatttatt ttgttttcgc tgtagtgaat aaacgccgat
     2401 gtatacgctt gatgtttctt caaaaaatac aaaaggacaa gaatacttgc tagcataccg
     2461 ataaatgccg cgaaagttga ttgcgtgacc gccgtcacat agtcaccatc catgactttc
     2521 atgatgatga atgtagccaa tagcatataa aaaatacgtg ccacctgttc tacaatctgc
     2581 gacagcgcat aaggcatcat ttcttgattg ccttgcaaat accctcgtat cacactcata
     2641 caaggaaaaa ccaatacagc gatactcaaa gaacgcatcg ttggaatcag ttcttctccg
     2701 ccaccagaag catgagctag ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg
     2761 cctaacccgc ccatcatctg taatgcacgg ataaataatc gtcgtgaagt gccatattca
     2821 ttcaatgaat tatagcgtgc agtttgtttt gcaatcgcag caggtattcc tgctgtagag
     2881 atcagtagaa acaatgcata tatattgtat cccatattga ataatccatt ggcagcctta
     2941 gcattttcgc ccatccaagc ataccaggga atgatataga cagcgcctag caatcttgaa
     3001 atgatattac tggcggtcat ccaagcagag cctcgtgcca ttttttcttg cggtgttaat
     3061 tgatctaatg ctggttttct ttgattatcc atcggtcaga ttccaacttt ctacaaaaca
     3121 ttactatcat tttaattggt aaaacagaaa ctggcaatcg attttatata acttttataa
     3181 gaacaattgt ttttttgaat tcggtttcgt ttttttggta aagtaaagaa atatacagcc
     3241 ttttatggct aacatttata ggagatatca atgacacaag aaataaaaaa actacccgtt
     3301 atatttttag gtcttttctt tatcacagtt ggattgaatt ggttcttgct ccctcacgat
     3361 atagcttccg ccggagtggg cgccatcgga catttagttg aaacggcctt ttctatccaa
     3421 cgctcagtta ctgtttggag catcaatctt atgatgctgc ttctagccgc tgtcctatta
     3481 ggaaaatcag tattttttaa gactgtagcg ggaagtctgc tttttcctgt tgttttagag
     3541 attgttccaa aagtggaaat gatctcctca catttctttt cactgatttc aggaagtctt
     3601 ttgttcagtc ttggggttta cacattgtac acagtcgggg cctcaaatgg cggtataacg
     3661 ataccgccga ttattttcca caaatttttt cggcttccaa tgccgcaagg acttttactt
     3721 accaatagtc ttatcgtctt tttgaattat tatgtttttg gattgttgga gacactattc
     3781 gttctcctat ctattacagt cagttcattg tttatgaaaa tactcatccg cttgtcccct
     3841 gtgtcaagaa aatttgaata aagctggatt ttgtcatagc ggaaacaaac gatatattaa
     3901 aaagagtgtg ctataataaa ccaagaaatt gaataaagcg agggatcaat caatgagctt
     3961 ctctttagaa gacatccgtc atttactatt aaaagaaaat ctattaaaag aatttgtttc
     4021 aacaagaggc tggcatcttg atgtaccaga aaaagctgaa tttcttcagt taagttatga
     4081 ttcaagaaaa gctgatgctt ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga
     4141 atatttggat tcagcaatcg aacagggtat tcaatattat gtatcagaac aaccatacga
     4201 aaattcagca gtaggaatca tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat
     4261 ggctttttac gactatccgc aaaataaatt gaaagtgatc ggattcactg gaacaaaagg
     4321 taaaacaact gctgcgtact ttacaaaagc aatcttggat cacacaacaa ataaaaaaac
     4381 agcattgctt tctactatga atacaacgtt agatggcaaa acatatttca aatcccattt
     4441 gacaacgcct gagtcattag atttatatcg tatgatgtca gaagcagttg aaaatggtat
     4501 gactcatcta gtgatggaag tttcttcaca agcttacaaa acacaacgcg tatacggctt
     4561 gactttagat gtcggaatct tcttgaatat ctcgcctgac catatcagtc cgatcgaaca
     4621 tccgacattt gatgactatt tctattgcaa gcgacaattg atcttgaatt caaaaacagt
     4681 tgttttaaat catgaaagtg actattttga tttacttagg gaaacagccg atctattcca
     4741 gattccaacg atcacttatg gtcgttcgga agaaagcaat tatcaagtga tccgttcaga
     4801 taaaggtact cacggtttta ctttatcttc tcatgaagat cgcttagcta tctgcgatac
     4861 tgcttatgat attttgctgg ctggaggatt caaccaagaa aatgcagcca gtgcgatcat
     4921 tgccgcagca ctaacaggtg cctccagtca agatgcacaa gacggattaa aagaagctcg
     4981 tgtaccaggc cgaatggatc aattgatcca atccaatgga gcgcatgtct atgtggacta
     5041 tgcacataat tatctaagct tgaaaacatt gctgaaattt gccaaaaatg aacatccaga
     5101 tggccgtgta attgttgtat taggcagccc aggaaacaaa gcaatctccc gtcgacatga
     5161 ttttggaaaa gtgctatcag aaacagctga catcgccttt ttgacggcag atgacccagc
     5221 atttgaagat ccgaaaaaaa tcgcggaaga aatcaatgaa gcaattacta atccagattt
     5281 gatcgttcat tatgaaatgg atcgtcctga agcgattcga cgcgctttag cagaaagcgc
     5341 tccgcaagat tcagtcgtta ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg
     5401 tgtagatgaa ccttatgaag gggattatgc aatcgtgaag cggttgattg aagaataatg
     5461 gtatttatat actagtggtt tcccatttct aagaaaaaaa gggactgtga ttttttgtca
     5521 cagttccttt tttcttagaa atcattttaa cttttcacta gctaattctg ctgccttttc
     5581 tctttcttca tttaataatt tattatcata gaatttgata aatgggaacc agataatgaa
     5641 tgcgatgatt gcacaaatga ctgctaacac agcagctttc caatcaccac cactaccaac
     5701 aaatgctccg atcccgactg gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat
     5761 ttccagccga atggcaaaat aagcgagtgt agcagaagcc atcggagcta agaaaaacgg
     5821 aaccgctaaa tagggattgt agacgattgg catcccgaaa atgattggtt cattgatatt
     5881 gaataatcct ggtacaagtg aggcttttcc taacactttc agctgatcag atttcgcgag
     5941 gaatgcaata aagatgatca atcctaaagt cgcacctgat ccaccaacgg ttacataaga
     6001 attgttgaat tctcctgcca acggataatt tgcgccttgt gcgttagctg ccatattagc
     6061 taatacgatc ggggtcaaga aagaagtgat gatattcgct ccatggatcc ctacgatcca
     6121 caatgcatgg atcaagaaat aaatcaccat gacaccaagc catgtattcg tcaactgcgt
     6181 aacaaaccca aatgggatag caatcatttt gaagatatcc gttcctagtg caactaataa
     6241 tccattgatg atcatcacca caaacgcaat gacaaatgtt gggatcagag cagtgaaaga
     6301 acgagaaact cctggcggaa caacatctgg catttttatg acccaatttc tcttgacaca
     6361 catacaatac aattcgaccg caataatcga cataataata gctgtaaaga ttcctgaagt
     6421 tcctaaacgt tcgacaaaag agcccatcct aataccattt actacagttt ctgtctcatt
     6481 gatagaagca atcaagctga ttttaccatc agtcatgatc aattcaggga tacacataaa
     6541 gaatgcaaaa acagatagta atgcccccgt gagcggatta acatttaatt tttgttcatc
     6601 tcgttcaatt gacgttaatt cataagcaaa cacaatggca aagtaaatag ataaaatccc
     6661 catcgtcatt gtattgacga tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa
     6721 aaatccttct aaaaatggga acggtaatgg caaaatattt aaaaccaaaa acatcgagcc
     6781 tacaatcgtg aaaggaatag aagccatccc cgctgccatg acagcccgaa cgattttaag
     6841 ttgcgcaatt ttccccatag gtcccatcag gtatttctgc aaaaaatcaa acattagtta
     6901 ttctcctttc ctaaatataa cgctttttca tattgtttaa tacggatata ctgttggtct
     6961 tggttgagtg aaattttctt taaacgatac tgtataaatg cactggcaaa aataccgctt
     7021 acaacaagaa tgaaaacaaa taactgtcct ccattgttat ttgccataaa aggatacaag
     7081 aatgtaaatc ctgaggtaaa caacactgga atcactaata aattaaagac actctggaac
     7141 aaaaaatacc actttgtcca tggaacgttc gtttggtgtt ttcgataaag ctttacttgt
     7201 tctcctactg caggcaaaat agctaaaatc agaatcatcg gtaaactact tcctaaactt
     7261 ttagctgata gaagcagaat catccattgc atgttcatga aaaagaaaaa ggcagtcagg
     7321 taacgtacga ttagataacg attgaaatac atgtttttca aactaagttg ctttttttct
     7381 aattcgattt tcctttttac tgtatcctgc acaatgatcc ttcctttcta tttatttttt
     7441 tgatacaaat aattcatttc ctgtgcaact tccaataatg tcatcgtcgt catcagatga
     7501 tcttgtgcat ggaccatgat aatttccatt tctatttttg tcccgctggc atattctttc
     7561 agaagttgag tttgtgactg atgcgcatga agaatctctt catttgcttg tcgcaacttc
     7621 tcttctactt cttcaaacat ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc
     7681 cgtgcatttc cgctatggaa aatgatatca aaagaagtca cttgtagtgt ttcactgttt
     7741 attttttcag ccatctgttt tcctccttat tcgatcagtt tgataaattt actgctgaaa
     7801 ttctcaaaaa taggttcagc taagatttcc tgctgtactt ttagctggtc aactaaccta
     7861 acgattgctt tagtcacgac cgtaattcct tcattttcga cataagaagg cgaaactaaa
     7921 aaaacaaaat gaatttcttg atattgatcc cactgcatcc cagaaggaat caatgcaacg
     7981 gctacctttg tttcgattcc aactggaaca gcgggatgtg ggactgccac tgtatcactg
     8041 aatatgattt gtcccatatt ttcacgttgc tggatctgct ttcgcatttc tcttatatat
     8101 ccttcttctt catttatgct cagcttttgc agcaaatcat gaagaatttc ttcctttttc
     8161 ggttcgtacg tataagtctt gaaaaatttt tcagacaact gttcggtgta aatctgcttt
     8221 ttttgctgta gagaaaatgc tggtatcggt ctatctgagg aatgataggg tatagattct
     8281 ccaactacct ttcgtatttt acgaacatca tcttcattca aaaaaatact gacatgtaaa
     8341 actggaattt tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag gtcgattcct
     8401 tttaaagttg tttcattgat ctcataatag ccttttacac ttaccactgt aatattttta
     8461 ccgaattcac tgactacgcg attctttaat agttgggcac tgccgtaacc cgttgcacag
     8521 ataatcaatg catgtagttt atgcgcagct ctttcttttt ccaatgccgc catgagatgc
     8581 aaagccaaat aggcccattc atcttcattg atggtatagt ttttcaaagc cggcatgttg
     8641 ctgaaatgcc tttttactgc gtcaaagact ttgggatttt ctttctttat ctcctcagtc
     8701 aatgggttct caatgcgaat acctcggctc aatcgaatca tcatcggtga caaatgatcg
     8761 agtagtccat tgaaaagctg gagatcctct actaatgaaa aatgaaattc ctgagacaat
     8821 cttttcagaa ctttcgctaa ttcctttgat agttcctgat cattttgatt ttctttgtga
     8881 ttcgacttag ccatgagatg taatgtcaaa taagcaattt cttcttcggg aaacgataca
     8941 cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca cttgatactc tactcgctta
     9001 gaaacttctt gttcgatgcc taattccttg atttcaaacc cttctcggag ccgtttgatc
     9061 cccaaagcca agtgtaaagt cagattctgt atgataaaat ccgaaacttt taacttagct
     9121 tctcttgttt catcgagaat gataatgact agctcttcaa aactgatgtc tgggaaaaaa
     9181 cggctttgcc ctaaatattc tttcaatgaa tttccataat ggtttccaaa aaaagtatcc
     9241 atgataaaat gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg accatgctta
     9301 gaaacgattt ttagtgaata tggaatcaat cgctccttga tttcttgcat gtcctttgtc
     9361 aaactggaac gactgatata aagctcttct gccaaatcat ccataaaaag aatggcatct
     9421 tccaaaagca attggttcaa tatatacttt tgtcgatctt cggactcata aaatccttgc
     9481 ggttctctag catatccagg tactatttct tgccgactca aaaacaagtc aaatgtcagt
     9541 ctttgtaaaa tatgcagttg gtagccataa cctggtttag caatgatctt acctccgttt
     9601 ttttctacta actctttgat ccgctgcaag taattacgga ttgtccgatc agataataac
     9661 aattcagaag ctaaacgttg actggtgaca tactccttct tgtgatcaat caaatatctc
     9721 aacagcaatt tttccttctg tttcattatc atccactcct attccatcat atgttctctt
     9781 cgatcacctc tgctaacttc tggatgcctg tagggattgg gacatacgct tggaaaggga
     9841 tactaacaac aggctttcca acagattctc ctaatttttt gaatttatcc aaaaacatcg
     9901 tcgtttgcgg gctgatcaga aataaatcaa aatcactatt cttaatcata ttatcgcctt
     9961 ctgtagctga aactgcatct acgagtacct cttctctttt ttgcttaaag aaatccgttg
    10021 tctttgaggc catcatcgag gatgacattc ctgccgcaca aataatcaat gcttttttca
    10081 taaatcaatc actcctttta tttttataaa ccaagcatat cttgtaagcg ttttctattc
    10141 aaaatatata tttccgtagc attgcggaaa aaatgtgtat catatcttga ataataattt
    10201 ctggcaccgt gatatactag tgatagaatt ttttcaggag gggatttagt gagtatcaca
    10261 gtgaatattt tgtatacaag tacaaatgga caagccagaa aatttgcaga agaaatggtc
    10321 gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata tgactattac
    10381 tttccactag aagatccaga atctctttta ctgatcgacc gttggaaaga tgaagaagca
    10441 atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa acgaactccg taaaaagtat
    10501 aaattaaaaa tgaaagtcga acgtttcact gatttaccgt aaaaagatac acatggaatg
    10561 atagcaattt atcatccatg tgtatcttca ctttttcctt attttttatt cgatctcatc
    10621 taatgattca aaccgtgttt ttctcttatt tttcggataa gtatttcagt caccccaata
    10681 tacatgattc ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt tactgctcct
    10741 ggaaccccga ataaactggc agtgattgcc aaagcaccaa aagaattaaa aactgtttta
    10801 tatctatagg tagaacaaac acctaaagct agaccaccta atataccgac ccagttaaaa
    10861 ggaataaatt ggctgatacc aataaataaa acacatccaa tcaatgagcc aatgattcgg
    10921 tctactgctc gttctttgaa accaatcgac atcaatccat agctagatac aatagaagaa
    10981 aatgcaaacc ctgcccacat caatcgctga aaagggacat actctcctgc aacgatcaaa
    11041 agactaatgc caaatgcata gatgagcatc caaatttttt cctgcgaata catgcctttc
    11101 aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg
    11161 agaactgaaa accaacaaaa aaacaaaaat aataagctac ttgtttgtgt aaagaaattc
    11221 ttgttcaacg aatctttagg taacgaataa acaataaaga gatatgaaaa gccgtacaaa
    11281 gaagcattcc ccattttggg atcactagcg gttgccatta atatgctgct tagtaaaata
    11341 aaatgaatca acatgcccat aactgaccat ttgatcagct ggacatacgg tgctactaat
    11401 agtgagaaca tgacgattgc taaacctatg atcgaatggg aaatcttata accaaagtca
    11461 acaaaacgaa tactcatcaa catacagaat aaaaccacag cgtatggttt atgcttcgat
    11521 tcaaataatg ttgtaattat aatgataaaa caaatggcaa acaatacgat gaataatgat
    11581 cgtaaaaaca atgaacaaat atacatgcac ttttcttttt tagaagctgc ttctcgtatt
    11641 ttttgtttta aaataaacgg atctagttgt agagactgat aaaaatccac ctgttgtcgt
    11701 cctttctttt tacttttatt ttttacagtc atctatgaca ggttgtaaat actatggaac
    11761 atcatatggc gttgtcattt tcttgtcaac tatttttcct attcatgttt ttataaaaaa
    11821 acatatcgtt ttcttgtatg attaaatcac cacaaaataa acaactgaga gaacgatatg
    11881 ctttatgaat ggttctatca aaaaaattct gagatcaata gacaaaaatt tgatgattac
    11941 agaggtctct tacgaaacct tacagaagaa aaaaacattg atcgttgctg cagttctctt
    12001 ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt
    12061 aatggtggtg aaaaacaaga agaaggaaac cattgtccac tttgaataat ataatcatat
    12121 gccaatggtt atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc gaagccattg
    12181 gaacgctcaa agttatttta tccgtccaag gcactcgatt tcaaatcatg tcagacataa
    12241 aatcacttct ttactgactg aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt
    12301 atctttgaca accgttattc gcacattgaa agagttgaga agctatttac caaagtcttc
    12361 caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt ttccgttgag
    12421 gataaactga gttttatttc cgcggatggc gagacagaaa aattaatcga gaaaatttac
    12481 ctacacaaaa aattcctcga ttaacaaact atttccttag ctgcactaat cctaaagaaa
    12541 tagaattctt agttacagac atgaacgcca cctactttca gctcaccaaa catgttctac
    12601 caaatgcgaa attggtgatt gatcgattac ttggtttttc cacgcctttc aaggaggcgt
    12661 aacctttatt cacgagttgg ttgaagcttt ccgagacaaa agacccgact tgtttttctc
    12721 cttattgaca aagagccatt aaaactagag aaaaaattac aactgatcgc tgattatttg
    12781 tagaaagaaa atcaaagtat tacccaataa acttgagtca ttaggcaaaa aacactgcga
    12841 aactcaagaa tgattttcgc agtgtttttg tatatttttt attaactagt attactacaa
    12901 aggtttattt ttcacttctc aacgcagcac aaaattgtct ttacttattt actggttggg
    12961 ttcaaccttc tctactccga tactaatatt tgtttgtata ccccttctaa aatcggtagt
    13021 ttccattcaa ttgatgggta ctagttgatt ttaaaaacta tgcttattca attttttatt
    13081 ttagctcata tatttcgttt ctttctaaat tttatactga tgaaaacaag tagcacaccc
    13141 aatattgaca atataaactg gctaatgatg gtttctccag tttttggaag atgcttattt
    13201 ccactgcttg ttggattgtt tcctcttata tctgagctat cagcaacttt tctattctct
    13261 tttgttaaaa agatcgccac ttcttgttct ttttcgatct tgaaagttac tggtgtcgca
    13321 tccaattcat accccgttgg cgcttgagtt tctaccagct gatatgctcc tggtgctaag
    13381 ccatctatcg ccagttttcc atctgctcct gtggttagac ctgactgcag aacggttcca
    13441 ttggcatctt gtaactcaaa aacagctcct tgcaggactt ctccgctttg gtcatcgatc
    13501 tttgtcaaca cgacaccgcc tggtgtaagt cgattttctt tcgtcaattc taccaccgct
    13561 gtctgacttc tttcgatctc aaactcgatt ggtgtcgcat ccaattcata ccccgttggc
    13621 gcttgggttt ctaccagctg atacactcct ggtgctaagc cgtcaatcgc cagttttcca
    13681 tctgctcctg tggttagacc tgactgcaga acggttccat tggcatcttg taactcaaaa
    13741 acagctcctt gcagga
//
LOCUS       DAHUWM010000066        13701 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHUWM010000066 DAHUWM010000000
VERSION     DAHUWM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.066"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..902)
                     /locus_tag="NK788_001335"
     CDS             complement(<1..902)
                     /locus_tag="NK788_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCH6769673.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="NK788_001336"
     CDS             1185..2153
                     /locus_tag="NK788_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6769674.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="NK788_001337"
     CDS             2266..2544
                     /locus_tag="NK788_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCH6769675.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="NK788_001338"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="NK788_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCH6769676.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="NK788_001339"
     CDS             3001..3756
                     /locus_tag="NK788_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6769677.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="NK788_001340"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="NK788_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCH6769678.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="NK788_001341"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="NK788_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCH6769679.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="NK788_001342"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="NK788_001342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCH6769680.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="NK788_001343"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="NK788_001343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCH6769681.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="NK788_001344"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="NK788_001344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCH6769682.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="NK788_001345"
     CDS             8493..9035
                     /locus_tag="NK788_001345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCH6769683.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="NK788_001346"
     CDS             9058..10614
                     /locus_tag="NK788_001346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCH6769684.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="NK788_001347"
     CDS             10628..11530
                     /locus_tag="NK788_001347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCH6769685.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="NK788_001348"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="NK788_001348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCH6769686.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="NK788_001349"
     CDS             12973..13395
                     /locus_tag="NK788_001349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCH6769687.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2451 c         2988 g         3732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag a
//
LOCUS       DAHUWM010000067        13615 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHUWM010000067 DAHUWM010000000
VERSION     DAHUWM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.067"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            42..284
                     /gene="yaaA"
                     /locus_tag="NK788_001350"
     CDS             42..284
                     /gene="yaaA"
                     /locus_tag="NK788_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCH6769688.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            271..1395
                     /gene="recF"
                     /locus_tag="NK788_001351"
     CDS             271..1395
                     /gene="recF"
                     /locus_tag="NK788_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCH6769689.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1392..3338
                     /gene="gyrB"
                     /locus_tag="NK788_001352"
     CDS             1392..3338
                     /gene="gyrB"
                     /locus_tag="NK788_001352"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCH6769690.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3358..5829
                     /gene="gyrA"
                     /locus_tag="NK788_001353"
     CDS             3358..5829
                     /gene="gyrA"
                     /locus_tag="NK788_001353"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCH6769691.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6065..6364
                     /locus_tag="NK788_001354"
     CDS             6065..6364
                     /locus_tag="NK788_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCH6769692.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6414..6926
                     /gene="ssb"
                     /locus_tag="NK788_001355"
     CDS             6414..6926
                     /gene="ssb"
                     /locus_tag="NK788_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCH6769693.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6952..7188
                     /locus_tag="NK788_001356"
     CDS             6952..7188
                     /locus_tag="NK788_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCH6769694.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7331..9304
                     /locus_tag="NK788_001357"
     CDS             7331..9304
                     /locus_tag="NK788_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCH6769695.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9310..9762
                     /gene="rplI"
                     /locus_tag="NK788_001358"
     CDS             9310..9762
                     /gene="rplI"
                     /locus_tag="NK788_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCH6769696.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10036..11403
                     /gene="dnaB"
                     /locus_tag="NK788_001359"
     CDS             10036..11403
                     /gene="dnaB"
                     /locus_tag="NK788_001359"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCH6769697.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12183..13475
                     /locus_tag="NK788_001360"
     CDS             12183..13475
                     /locus_tag="NK788_001360"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCH6769698.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4535 a         2466 c         2996 g         3618 t
ORIGIN      
        1 taatagagta tacttttaat aatgaaaatg agggatgggc attgaaacaa caagtcattc
       61 taaatacgga attcatgacg ttgggccaaa tgttgaaaga agtcacagtt atcggtagtg
      121 gcggacaagc aaaatggtat ttggcagaaa acactgtgtt agtcgatggc gaaccagaaa
      181 atcgtcgtgg acgcaaactt tatcccggca tgatggttga agttccagaa gtaggaactt
      241 tttttatggt aaaaaaagga aacgacgata atgaggctga ataagctgta tttaaaaaat
      301 tatcgaaatt acgaagagct gaacatcgaa ttttccaaga acctcgtcat ctttttagga
      361 gaaaatgcgc aaggaaaaac gaatcttttg gaaagtatct acgtattagc tatgacgcga
      421 agtcatcgga caagtagtga gcaggaattg attggctgga ctgacgatca ggcgatgatt
      481 caaggagaga tcacaaaagg ttcatccact cttccattag aaatcttatt atctaaaaaa
      541 ggccgaaaaa ccaagatcaa ccatatcgaa cagaaaaagt taagtactta cgtgggacaa
      601 atgaatgtca ttttatttgc accagaagat ctttctttag taaaaggcag cccgcagacc
      661 aggcggaagt ttttagatat ggaattaggc cagatcaatc caatctatct ttatgatctt
      721 gtccaatacc aagcaatttt gaaacaaaga aaccagtact tgaagcaatt gaatgaaaaa
      781 aaacaaactg atttgcttta tttggatatt ttgtccgaac aactagcagc gtttggcagc
      841 aaagtattga aagcaagagc acagttcgtc aagcgattgg agttttgggc taattctttg
      901 catcagcaaa tcacccatca aaaagagcag ctggaaatcg agtatctcac cgcagtcgat
      961 tctttagaaa cacatacgca agagcaaatc caagaacaat ttctcgcact tttgaaccaa
     1021 aataaaaaga aagacctttt tcggggaaca acgacagttg gtcctcatcg tgacgatctt
     1081 tcctttttta ttaatcagaa aaatgtgcag acttatggat cgcaaggaca gcaaagaaca
     1141 actgcactaa gtgtcaaatt agcagaaatc gatttgatta aagaagagac aggggaatat
     1201 ccaatccttt tgttagatga tgtgatgagc gagctggatg ataatcgtca actccatctg
     1261 ctagaaacaa tcgaaggaaa agttcaaacg ttcttaacaa caacgacact tgaccatgta
     1321 aaaaataaaa tgactgttga acctgagatt ttttatgttc aacaagggaa actaaaagga
     1381 gaagaaactg aatgacagaa gaaagaagtt tagtagaacg cgctaaagag tatgatgcca
     1441 gtcagattca agtacttgaa ggacttgaag ccgttcgtaa acgacctggc atgtatatcg
     1501 gttcgactag ttctgagggt ctgcatcacc ttgtttggga aattgtcgat aattcgatcg
     1561 atgaagtatt agcaggtttt gcaacgaaaa tccatgtgat tattgaaaaa gacaacagta
     1621 tcaccgttat tgacgacggt cgggggatac ctgtagatat ccaggcaaaa actggacgtc
     1681 ctgcagttga aacggtcttt actgttttac atgctggagg aaaattcggc ggtggtgggt
     1741 acaaagtttc tggtggactt cacggagtag gatcttctgt tgttaacgcg ctttctacac
     1801 agcttgatgt caaagtatac aaagaaggaa atgtgtacta tcaagaatat cgtcgtggag
     1861 ctgtagtgga cgacttaaag attatcgaac aaacggatcg ccatggaaca acggttcact
     1921 ttacgccaga ccctgaaatt tttacagaga caactgagtt tgatttttca aaattggcta
     1981 cccggatccg tgaacttgct ttcttgaacc gcgggatgcg tatatcgatt gaagacaaac
     2041 gagaagaaga accagtcatc aatgaatacc attatgacgg cgggattaaa agttatgtcg
     2101 aatacttgaa tgcaaacaaa accgtgattt ttccagaacc tgtatatttg gaaggtgaac
     2161 agcaagatat cgcagttgaa gtctctatgc aatatacaga cggctaccat tccaatatca
     2221 tgagttttgc caacaatatc catacgtatg aaggcggaac acatgaatca ggattcaaga
     2281 ctgctttaac tcgtgtgata aatgattatg cccgaaaaca aaagctgatg aaagaaaatg
     2341 acgacaactt gaccggtgaa gacgtgcgag aaggattgac agctgttatt tctatcaagc
     2401 atcctgaccc tcaattcgaa ggacaaacaa aaacaaaatt gggcaattcc gaagtacgga
     2461 cagtcactga tcggttattt agcgaacatt tcatgaaatt cttgctggaa aacccaagtg
     2521 tgggccgtca aattgttgaa aaaggattac tagcttccag agctcgtcta gcagcgaaac
     2581 gtgcgcgtga agtaacgaga cgtaaaggtg ctttagagat tagtaatctg ccaggaaaat
     2641 tagcggactg ctctagcaac gatcctgaaa aatgcgaatt gttcatcgtc gaaggagatt
     2701 cggccggcgg atcagctaag caaggtcgta atcgagagtt tcaagccatc ttgcctattc
     2761 gtgggaaaat cttgaatgtt gaaaaagcaa gcatggacaa gatcttggca aatgaagaga
     2821 tccggtcttt atttactgct atgggtacag ggtttggaga agattttgat gtatcgaaag
     2881 ctcgctacca caaattagtt atcatgactg atgccgatgt cgacggcgcg catatccgta
     2941 cattgctgct gactcttttt tatcgttaca tgcgtcctat tgttgaagct ggatacgtct
     3001 atattgctca gccaccttta tacggggtaa aacaagggaa gaatattacg tatgtacaac
     3061 caggaaaaaa tgcagaagaa gagttgaaac aagtcgttgc ttcacttcct gcctcaccaa
     3121 aaccaagcgt tcagcgttat aaaggacttg gagaaatgga tgatcatcaa ctatgggaaa
     3181 caactatgga cccaagcaat cggatgatgg cccgcgtcag tgtggacgat gctattgcag
     3241 cagatcagat atttgaaatg ttgatggggg atcgagtaga accccgtcgt gcattcatcg
     3301 aggaaaatgc acactatgtg aagaacttag atatttaatc atgaaggagg aaaaagaatg
     3361 agtgaagaaa tcagagaaaa catccatgat gtcaatctaa ccagcgaaat gaaagactcc
     3421 ttcatcgatt atgcgatgag tgttattgtc gcacgtgcac ttcctgatgt cagagatggt
     3481 ctgaaacccg ttcatcgccg tattttatac ggcatgaacg aattaggtgt aacaccggat
     3541 aaagcccata aaaaatctgc cagaatcgtc ggagatgtca tgggtaaata ccatcctcat
     3601 ggagactatg cgatctatga gtcaatggta cggatggcac agccattcag ctaccgttat
     3661 atgctagtgg atggtcatgg aaactttggt tctgttgacg gtgacggtgc cgccgctatg
     3721 cgttataccg aagcacggat gagcaaaatc gctacagaaa tgctgcgtga tatcaataag
     3781 aacactgttg atttccaaag caactatgat gatacagaaa aagaaccagt tgtactacct
     3841 gctcgcttcc cgaatctttt agtgaacgga acaacgggga tcgccgtagg tatggccacg
     3901 aatatcccac ctcataactt gtctgaggta gtagcggcta tcgatttgct gatggataat
     3961 cctgatgtga cgaccaatga attgatggaa gtattacctg gaccagattt tccaacaggt
     4021 ggtttggtta tgggaaaatc aggaatccga agagcgtatg aaacaggtaa gggttctatt
     4081 accgtacgtg caaaagttga gattactgag atgccaaatg gtaaagaacg gatcatcgtg
     4141 acagaattgc cttatatggt caataaagca aaattgatcg aacggatttc tgaactgcac
     4201 agagacaagc gtatcgaagg gatcacagat ttgcgggacg aatcttcccg tgaaggtatg
     4261 cggatcgtca ttgacatccg tcgtgacgcc agcgcatctg ttatcttgaa taacttatat
     4321 aaattgacat ccttgcaaaa ttcatttgga ttcaatatgt tggcgatcga aaaaggtgtg
     4381 ccgaaagttt tgagcttgaa acaaattctt gaaaactata tcgaacatca gcgcgaagtc
     4441 atcacaagac ggacagtctt tgaaaaagaa aaagcagaag caagggcaca cattttagaa
     4501 ggtttgcgta ttgctttgga tcatatcgat gaaatcattg cgattatccg cgggtctaaa
     4561 tcagatgacg aagcaaaagc gacgatgatc gaacggttcg atttatccga tcgccagtct
     4621 caagctattt tagatatgcg tttacgccga ttgaccggtt tggaacggga aaaaatcgaa
     4681 aatgaatacc aagaactctt aaaactgatt gaagatttaa ctgacattct ggcaagacca
     4741 gaacgtgtta cagaaatcat caagaatgaa ttagcagact tgaatcaacg atttggtgat
     4801 aaacgacgca cagaattact agtcggtgag gtcttaagtc tggaagatga agatttaatc
     4861 gaagaagaag aaatcgttat tacattgaca aacaatggtt acatcaaacg tttggcaaat
     4921 aatgaattca gagctcaacg aaggggtgga cgtggtgtcc aaggaatggg tatccacaat
     4981 gacgacttcg tcaaaaatct ggtttcttgt tctacccatg atacattgtt attctttacg
     5041 aataacggaa aagtgtatcg ggcaaaagga tacgaaattc cagaatacgg gcgcacagcc
     5101 aaaggaatac caattatcaa tttactaggt atcgattcga gtgaaagcat ccaggcaatc
     5161 attgcggtga cgggagaagc agaagaaggc cactatctct tctttacgac gcgtcaaggt
     5221 acagtaaaac gtacttctgt cactgctttt gctaacatcc gaagtaatgg tctgatcgca
     5281 attggtttga aagaagacga tgagctcgtc aatgtgctac tgacagatgg tcaatctaat
     5341 atcattattg gaacacataa cggctactct gtcactttca aagaagaagc agtccgcgat
     5401 atgggacgta cagcttccgg ggttagagga atacgcctgc gtgaggaaga ttatgtcgtt
     5461 ggggcagcct taatgaagga agatcaagaa gtattagtga tcacagaaaa aggttacgga
     5521 aaacggacaa aagtatcgga atatccagtg aaaggccgcg gaggtaaagg gatcaaaacg
     5581 gcgaacatca ctgaaaaaaa tggtccacta gctggattga ccactgtttc tggagatgaa
     5641 gacatcatgc tgattacaga taaaggtgtc attatccgtt tcaacgtatc gactgtttct
     5701 caaactggac gttcaacatt aggcgtacgt ttgatgaaga tggaagctga tacgaaagtt
     5761 gtcacgatgg cagcagtcga accagaagct gcagaacaaa cagttgaaga acaaggatca
     5821 gaagaatagc catcaagact aattgtgtaa gctagcaaga atgaccgatt aaaaaatctg
     5881 gttattcctg ctggcttttt tgattagttg aatttctctt aaaatattcg aaactgttca
     5941 ttctgtattg cttttgacca aaatagagtg tataatcaga gcttgtgagt agtctaatta
     6001 attagataac tctccttgct cttaggaacc taagagccga aagtccacag ggaggtgaat
     6061 atcaatggaa aacacgaaat atgaaatcat gtacattatt cgtccaaaca ttgatgaaga
     6121 agcaaaaact gctttaatcg aacgtttcga tgcaatcttg aaagataacg gagcagaagt
     6181 tatcgaatca aaagattggg aaaaacgccg tttcgcttat gaaatgaacg gttaccgtga
     6241 aggtatctat cacatcgtta aagtatcttc tccatcatca gcaaatgctg tcaatgaatt
     6301 tgaccgtctt gctaaaatca acgacgatat cattcgtcac atgattgtta aagaagaagc
     6361 ttaattgttg tttcatgtga aacaatagaa aacgatgaga ggagatgact attttgatta
     6421 ataatgttgt attagttgga cgtctgacga aagatccaga tttgcgttac acagcaagtg
     6481 gtacggcagt cgcaacattt actttggctg tgaatcgtaa ttttacaaac caaagcggca
     6541 atcgcgaggc tgattttatc aactgtgtga tttggcgcaa atcagcagaa actttagcaa
     6601 actatgctcg taaaggaaca cttttaggtg ttactggacg tatccaaact cgttcttatg
     6661 ataatcagca aggacaacgt gtatacgtga cagaagtagt agctgacaac ttccaattgc
     6721 tggaaagtcg ttctgcatct gaaaatcgcc aacaaagcgg cggattccaa agttctggtc
     6781 aatcagctgg cggatttggc ggaaacaata actctaatca aacatctcaa tcatccaacg
     6841 gtatgcctga tttcgaccgt gatacttctg acccattcgg ctcaagttca acgatcgaca
     6901 tttcagacga tgatttacca ttctaaacga atttgatagg agggaaatag aatggcacaa
     6961 caaagaagag gcggacgtaa acgtcgtaaa gtagactaca ttgctgctaa ccatattgaa
     7021 tatatcgatt ataaagatat cgaattacta aaaagattta tttcagaacg cggtaaaatc
     7081 ttgccacgtc gtgtaactgg tacaggcgct aaaaaccaac gtaaaattac tgttgcaatc
     7141 aaacgcgctc gtatcatggg cttgctacct ttcgttagcg aagactaata aatcctagga
     7201 attgaatgac tgatcctttt gatcagctat tcaattcctt tttttctgta aaaataacct
     7261 ctattcgttt agtcgctcga atgacgaaag ttatggtaaa atcaaattag tcatatatgg
     7321 gaggaaaatt atggaaaata aaaacaatca tttatccccg aaacctctct tccttatcct
     7381 gtttatatta atcgaagtat tggtagtctt aattttacca tttcgttttg ttagcgcttc
     7441 ggtcattcta ttgatgaatt tatggggtgt tcgctattgg ttgaaaatga aacgttggct
     7501 tattttaaat aaacgtgaaa aaatccagca ggcttcgact gtagctgaaa ccaatctagc
     7561 ttatgtctca gaagtcatgc ctgtaggagt gattgcttat cagccatcaa gccaaaaaat
     7621 cgaatggctc aatccattag cattgaaaat caaagatcaa agtggattaa cagaggaaga
     7681 attgctagat acttttttta ctgcaaaaga aaaaaagaaa aaccaagtct atctagcaga
     7741 taaaacattt cagtttgata tggatacagt aaagggtgtt gtttatttta atgatattac
     7801 ccaaaaccag ttgttaaaac tccatcagac gaacagtcag ccggtgattg gtattttatc
     7861 tattgataac tatgcagatg ccattgataa attggatgac aaagagattt cttatttgaa
     7921 ctctttcgtc actacactag tttctgactg gatgaatgaa taccatgtct ttttcaaaag
     7981 aatcaacgca gaacggtttt tcttcgtagc acagatgatc gatctgaaaa agatgatgga
     8041 taaaaaattt gatttgctgg atcgctttcg agcagaagct gaaaaacaga gctcgcctat
     8101 cacccttagt atggggattg cttatggctc aagaaccctc gatgaaatcg gtggagaagc
     8161 tcagaataat ttagatgttg ctttagtccg tggaggcgac caagttgtcg tgaaagaggt
     8221 aaaagaagga gcaaaacctg tttattatgg ggggaaatct gctaagaatg tcaaacgcac
     8281 gcgtgttcgt tctcgagcga tgagtaccgc tttgcgtcgg atttttgatg aaacaggcga
     8341 tgtgtttatc atggggcaca agtttccgga tatggatgct ttaggctctg cttttggtgt
     8401 cagttgtctg gcacgcttca accagaaaaa agcttacatc gttatcaacg agaacgagat
     8461 cattccagat gtagaacggt gtcttgaaga aatccataag catcctgaat tagaaatgca
     8521 actaatctct ccagaacgtg ccatagagct gaaaaaagaa gaggacctcc tcgtgatggt
     8581 ggattaccat aaaccttctc tatcgatctc tcagggccta tatgagcaat ttgataagat
     8641 tgtgatcatt gatcaccatc gtcgtgggga agagtttcca agtaaacctc tgctgactta
     8701 tatcgaatct tcagcttctt cagcttcaga gctagtttct gagctgatcc aataccaaag
     8761 cagcaaaaca aatcgcttag atcgttttga ggccacatta ttgcttgcag ggatggttgt
     8821 agatacaaat agtttcaatg tccgtacaac tgcacgaact tttgacgttg ccagctattt
     8881 aaggtcttgt ggagcagacg catccttagt acaatattta ttgagctcgg atttagcgac
     8941 atacttggag atcagcaatt tgattgctgg aagcgactat gtgacttctg atattgttgt
     9001 ggcggcaggg acagacgaaa aagagtataa tagtgttata gcagcaaaaa cagcagatac
     9061 attgctttcg atgatcaata tcaatgcagc tttcgtcatt acaaagcgga cagacggctt
     9121 gattgggatc agtgccagaa gtaccggcac gatcaacgtc caaatcatca tggaatcctt
     9181 aggtggcgga ggacacttca caaacgcagc tactcaactg gaaaatacga cagttgagga
     9241 aacaaaacag aagctatttg ctgcaatcaa agaaaatatg aatcaaatat atgataagga
     9301 gtaatccaaa tgaaagtaat ttttttacaa gatgtaaaag gaaaaggtaa aaaaggggac
     9361 atcaaagaag ttccagcagg ttatgcacaa aattatctaa tcaaaaataa cttggcaaaa
     9421 gaagcaacga aaagcagtat cagtcagtta gcaggacaaa agaaagcaca agaaaaacac
     9481 gaagctgaaa tcttggaaga agctaaaaag ttaaaagaat tcttggaaaa agaagatact
     9541 gtagtcgaat tgaaggcgaa agcaggggaa gacagccgtt tgtttggttc gatcccttca
     9601 aaacaaattg ctgaagggtt aaacaaacaa tacaaaatca aattagataa acgaaaaatc
     9661 gaattagaaa atccgatccg ttcactaggc tacacaaacg tacctgttaa gcttcatcat
     9721 gaggtgacgg ctacgatcaa agttcatgtc gtacaagaat aacaaatctt gatagcgatg
     9781 ttcagctggt aagtataacc agaaggaaaa aagacaaacc gaaatttatc tgagaaaaca
     9841 gctgcagcaa tgaaagcaaa agctgcaaat cgatttcaga ttgatttcgg tttctttttt
     9901 gggaacagca aaatagcata aagaaaacga gtataataaa agagaactat ttttttatta
     9961 cactttcggt acgaatgatg acttttctct cattttcttg taaaatgaag caattgaaga
    10021 caaggagcga tacgtatgaa tgaagtgtgg caggatcgaa tcccaccgca aaatattgaa
    10081 gctgaacaag cggttttagg ctctgttttt cttgacgcag aaacgatcat tgatgccatg
    10141 gagtacatag agccaaaaga cttttatcgt cgtggccatc aaattatttt tgagacaatg
    10201 atcgagttaa atgatcgtaa tgaagcgatt gacgtggtta ctgtcaaaga ccggctggaa
    10261 caagccaatt tgttagaaga tgccggtggt ttaagttatc tctcagactt ggcattagca
    10321 gttccgacag cagcaaatat cgtctactat gcgaaaatcg ttgaagaaaa atcattgctg
    10381 cgtacattga tccagacagc aacaggaatc gttacgaaag gattcgagca aggagaagaa
    10441 gttcaaacaa ttttagatga tgccgaacgc agtatcttgg aggtttcgga aaaaagaaat
    10501 cgaagcggct ttttatctat tgctgaagta ctgaactcca cgattgaaaa tatcgatcga
    10561 ctagctcaaa acaatgaaga aatcacaggt ctaccaactg gctatcaagg tctggataaa
    10621 atgacagcag gtttccagcc cgaagaacta atcattcttg ctgcacgtcc agcggtaggt
    10681 aaaacagcgt ttgcattgaa tattgctcaa aatgtaggaa caaaaacaga tcgttctgtt
    10741 gcgatattta gtttggaaat gggggcagaa tctcttgtaa atcggatgct ttgtgcagaa
    10801 ggttcgattg aggccagcca tttacgtaca ggtcagcttt cggaagaaga atggtcgaat
    10861 ctgatcgttg cgatggggag tctttctcga gcaaatattt ttatcgatga tacaccaggg
    10921 atcaaaatct ctgaaatccg tgcgaaatgt cggaaactag cgcaagaaaa aggcaatcta
    10981 ggcttgatat tgatcgatta tttgcagttg attgaaggta acggtaaaga gaaccgtcaa
    11041 caagaagtat cagatatctc tcgccaactg aaaaaactgg cgaaagagct gaaagtacct
    11101 gtcattgctc tttcacagtt atctcgtgga gtagaacagc gacaagacaa acgaccagtg
    11161 ctaagtgata ttcgtgaatc cggatcaatt gaacaagatg cggacatcgt agccttcttg
    11221 tatcgagatg attattatga aagagaaggc ggagatgacg atgatggaaa cgagccaaaa
    11281 aatgacaacg tcatcgaagt catcatcgag aagaaccgaa gcggtgcacg aggaacagtt
    11341 gaactactgt ttatcaaaga atacaataag ttttcgtcgc tttcgccaag agaagaattt
    11401 taaagaagag gttgtgagcc ttgagcaatc ccgagagctc tcgtaagaag atgcaaggag
    11461 taaccgcagg aacgactaga gtacttaatt taaacagatc tccaaaggga ttgtgacaaa
    11521 agtcttgtca cagtcccttt ttccgaataa acggtgttca atcagctgac ccttggtatt
    11581 agctcagcaa acgtcaaaat atgaggaact attttgacgt ttgtttctct aatactcagg
    11641 tcaaaacgct gatcttacga cctcttttcc ttaaacggtg ttctcctgcc aactcctcgg
    11701 tcacaagtca caatatcatc aaacatgaga agcagttttt tgaaattgct ccttgtgact
    11761 cagagttaaa cggcaggctc acaacctctt tcttaaacgg tgttcaaaag cttgcatctg
    11821 cggtaatacg cccaatcaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat
    11881 tacttgatgc aggacagctt tttcacaacc tctgattcac ggtgttccaa aagtagcttc
    11941 ttcgaaaaat aagtcgaaat ttcacaaaaa tttgagaagc aattttcgta cattccttct
    12001 tatttcttga agctgaccac ttctgttaca acctcttttt taataaccat tctaaaaaga
    12061 taaagttcgt tttttaagcg tttttataaa ataaaaataa aaatcattcg attttatttg
    12121 aaatatgtat ctattattga tacaatggtt caggattaaa taatatagaa cgaggtgttc
    12181 acatgtcatc tgtagtagta gtaggtacgc aatggggcga tgaagggaaa ggaaaaatca
    12241 cagactttct cagtgaaaat gctgaagtga ttgcacggta ccaaggtggc gacaatgcag
    12301 ggcacacaat taagtttaac ggtgtgactt ataaactgca tttgattcct tctgggattt
    12361 tttataaaga caagatcagt gtgatcggaa acggcgttgt agtcaatcct aaatcattgg
    12421 ttaaagaatt agcttattta catgaaaaag gtgtagaaac aagtaatttg cgtatttctg
    12481 accgcgcgca cgtaatcttg ccttatcata tcaaattgga ccaattacaa gaagatgcta
    12541 agggcgaaaa taagattggt acaacgatta aaggaatcgg acctgcctat atggataaag
    12601 cagctcgtgt aggtatccgt attgcagact tattagataa agaaattttt gaagaacgtc
    12661 ttcgtttgaa tctagatgac aaaaaccgtc aatttgtaaa aatgtttgat tcagaaccac
    12721 ttgattttga cgatattttt gaagaatatt atgaatacgg acaacaaatc aaacaatacg
    12781 tcacagatac ttccgttatc ttgaatgatg ctttggatgc aggcaaacgc gtgttgtttg
    12841 aaggcgcaca aggtgtaatg ctggatatcg accagggaac ttatcctttt gttacttctt
    12901 ctaacccagt agccggtggg gtaacaatcg gaagcggtgt agggccttca aaaatcaata
    12961 aagttgtcgg cgtctgcaaa gcctatacgt cacgtgtcgg tgatggacca tttccaactg
    13021 aattatttga tgaaacagga gaacaaatcc gcgaagtagg acgagaatac ggtacgacaa
    13081 ctggtcgtcc gcgtcgtgta ggctggtttg attctgtagt tatgcgccat tctaaacgag
    13141 tttcaggtat taccaaccta tctttaaact cgatcgatgt attaagtgga ttggaaacag
    13201 taaaaatttg tacggcttat gaattagatg gcgaactaat ctaccactat ccagcaagct
    13261 tgaaagagtt gaaccgctgc aaaccaatct atgaagaatt gccaggctgg tcagaagaca
    13321 ttacaggatg caaaacactt gcagaactgc cagaaaacgc acgtaactat gtacgtcgta
    13381 tctcagagct tgttggtgta cgtatctcaa cattttctgt cggacctgat agaacacaaa
    13441 caaacatctt agaaagcgtc tgggcgcaaa tctaaggata aaaacgacga ctgaatcagg
    13501 acgttcttac cagagcgagt cgaagtgttt atctatacga taataataac ttgaacactg
    13561 tttattcaga ataagggact gtgacaaaaa ccgtgtcaca gtcccttttt ccgaa
//
LOCUS       DAHUWM010000068        13605 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHUWM010000068 DAHUWM010000000
VERSION     DAHUWM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.068"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..580)
                     /locus_tag="NK788_001361"
     CDS             complement(<1..580)
                     /locus_tag="NK788_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCH6769699.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKK"
     gene            complement(828..1664)
                     /locus_tag="NK788_001362"
     CDS             complement(828..1664)
                     /locus_tag="NK788_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCH6769700.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1675..2604)
                     /locus_tag="NK788_001363"
     CDS             complement(1675..2604)
                     /locus_tag="NK788_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCH6769701.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2796..3149
                     /locus_tag="NK788_001364"
     CDS             2796..3149
                     /locus_tag="NK788_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCH6769702.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3223..3879)
                     /locus_tag="NK788_001365"
     CDS             complement(3223..3879)
                     /locus_tag="NK788_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCH6769703.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4105..6039
                     /locus_tag="NK788_001366"
     CDS             4105..6039
                     /locus_tag="NK788_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6769704.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6125..7348)
                     /locus_tag="NK788_001367"
     CDS             complement(6125..7348)
                     /locus_tag="NK788_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCH6769705.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7342..9240)
                     /locus_tag="NK788_001368"
     CDS             complement(7342..9240)
                     /locus_tag="NK788_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCH6769706.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9234..10352)
                     /locus_tag="NK788_001369"
     CDS             complement(9234..10352)
                     /locus_tag="NK788_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCH6769707.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10435..11268)
                     /locus_tag="NK788_001370"
     CDS             complement(10435..11268)
                     /locus_tag="NK788_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769708.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11261..12178)
                     /locus_tag="NK788_001371"
     CDS             complement(11261..12178)
                     /locus_tag="NK788_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769709.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12201..12380)
                     /locus_tag="NK788_001372"
     CDS             complement(12201..12380)
                     /locus_tag="NK788_001372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769710.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12405..13529)
                     /locus_tag="NK788_001373"
     CDS             complement(12405..13529)
                     /locus_tag="NK788_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCH6769711.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4247 a         2720 c         2236 g         4402 t
ORIGIN      
        1 attttttaat tgtgccgttt acgagaatct cttcatcttt caatactagg cctattttat
       61 ttttataacc tggcatgctc attagataaa ggatttcatc gtaatggata tagtgatcgc
      121 tattaccata acggacagtt gtccaccttt ttttcgtagc atgttgaaca ggatagtttt
      181 ctttttcttt caataagtaa ctttggatgc tttttttatt tttttcataa gtgaattctt
      241 ttatcaaata accactaggc gtaatctttc gattaataac agttattgct ttattcggat
      301 gagacgttag ataaacaatt ttacactttt cattcttttc tctaagcatt tcgcctagtt
      361 cgataccgct ataataggcg ttcaaatcga tgtctaggaa aaaaatatct gaggaataga
      421 tttcattatt tttaatgaat tcaacaaatt gtataacgtt caatccctga acgatctcaa
      481 atttccaagg acttaataaa attctggatt cttccaaaat ttctactagc attttttcaa
      541 aaaccaaact atcatccaaa ataaatatac gcgttaccat aaaaagtacc tccactcgat
      601 attttactat caaaatcatt tcatacaact tttcaacata catgttagga ataaattgta
      661 attctcttca caatcttttc caacaccttt gaaaccgata tcaaatactt tcaggatgct
      721 gcttctaaaa taaatatcaa acaaatgaat acatcatgca aaaaaagcca gacaaccgac
      781 gtccttgcgt gtcagctgtc tgagctatgt ttatttattt tgttctttta aaaagaacga
      841 tacacatact gatttttcgg agcagagact ttctcagcgt ttgtaacaac tacagatggg
      901 atttcacgct tgaatggttg ataatagtct gattggatgg ttccttctat tttataccat
      961 tcatcatttt taaccgttac tccttccggc atatgcgtca acaaaccaaa gactcctgaa
     1021 tctgccacac aatggataat gccgaatcgg aacaagaaga tatctgtatt gctatcgttc
     1081 ggtgtttggt aaacaaatcc ttcatagctg atttttttgc caatgaattc gctagggtaa
     1141 ttataaatta gctccataac ttctaaatag ttttcatctg tgatcttgat agtttgctta
     1201 tccccatact tttctttcaa ctcattcatt tggtctaagt agtctgattt gttgaaataa
     1261 atactggtat ccggctttaa gtattgtgtc tgcatctccg gatcccctac tgattcttta
     1321 cttactggga aattaaatcc tttggcttct acgatcgtag tgtcgagact aactgttgga
     1381 aacaacgtcc ccacgattac tggcaaagct agcagtacat aagcgattcc acgttgataa
     1441 ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt
     1501 ttgacccaca aatacagctg aactaaagcc aaaagagcag ataaaatcat tgaaagaata
     1561 gctaagtagc gataatggac attgatatat tgatcgagtt tgccagaaac ttgcaagtac
     1621 atcatcaatt cgaaataccc agataaaatc aaaaaccgca gcatattttc cctcctaaat
     1681 aaataatgtg aatccaataa cgctaagggc aatcagtccg atcagcgata aaataaagcg
     1741 tcctgtaaaa tatcgtttca tcatcagtag atttttgata tcgaccattg ggccaaagac
     1801 caaaaaggca gctacaggtg ctgtgccgaa cagacttaac aaagaagatc ctataaacgc
     1861 atctgcttct gagcaaagag acatcgtaaa ggccaataac agcatgatga taatggctaa
     1921 aatctttgtt gagccgagcg ttagcataat tctagttggt aaataagtct gcatcgcagc
     1981 tgcgattaat ccgccaatga tcaaataacg acctgtatca aaaaattcgt cgattgaatg
     2041 tgtcaacaca tgcccagttt gatggatcag ctgatttttt ggttcattct tctcactttg
     2101 ttcttcttca tggacatgat gatggtgatt ttctgctact ttagacgtga ttttttcttt
     2161 caatacagcg tctttttgaa aataggcaag ccagacacca atcacaaggg caacaaaaaa
     2221 gctgcctatc acccgccagc ttgccatctg ccaagaattg ccaaatgcga taaaagtgga
     2281 aaaaatcacg attggattga tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac
     2341 tggtacgcct tttttaacaa attgatgaac gatcggtacg atgccgcatt cacatgaagg
     2401 aaagaaaaat cctaagatcg aaccagtaaa aatagagaag atacgatttt caggaagcca
     2461 tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa
     2521 taatacgaaa ggtaatgctt caataacgat cgataagaaa atcgtactca tctgcaaaac
     2581 cgaatttgga ataaaagaaa acatgagttt cctccttttt atttctagtt cattcgcttt
     2641 gcaaatatac gcttttattc aaaaaaaaca agttgctctg taaattttta cacaactctt
     2701 attttttacc aaagcacttc aattgacttt ttctgttatt cccattaaat taaggtctca
     2761 tacatatcac ttctatcata aaagggagaa taaaaatgaa acaaaccact aacttgactg
     2821 aagttcaaga catactgcaa cagtcatccg ttgccgtcat ttatctttcg atgcctaatt
     2881 gcagtgtttg tcacgctgtt cgtccacgac tagaggaact tctcacacat tatgatattc
     2941 cggcacttca tttggatgca tttgaaacac cagaagtagc tagtaaattt gaagtgctga
     3001 ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa gcacggttca
     3061 tcgactttaa aaaaatccgt tatctactag atgaactgac agcagaagat gattcccttt
     3121 cttatgaaga aatcttccga acaaaataat cgttgtccaa acagaaaaaa gcgattgaac
     3181 aagtgaaagt tttgccttca tttgttcaat cgctttttat ttttattctg ctgtattttc
     3241 ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa
     3301 atcaacattt tggatcaata catctgacgg agcattcaat cgagcagcgg taaagttcaa
     3361 gattcctttg attcctgctt ccgttaactg attgactact tcttgtgctc tttcagcggg
     3421 tatcgttaaa attgctactt cgatttgttg gatcttgatt tgttctgaca tatcttccat
     3481 tggataaacg gggataccgt ctacgatgcg cccaacgatt tcctcattca catcaaaagc
     3541 acaactgata cgtatgctat tactttgatg gaatttgtat tttagtaaag cacttcccaa
     3601 gttccctaca ccgatcagcg caacatttgt caattgatcg tcgtttaatg tctttgcaaa
     3661 aaagtgcata agatcttcta catcataacc atacccacgt tttcctaatt cgccaaaata
     3721 agaaaaatct cgacggatcg ttgcactatc tacttgaacg gcttcactta attcagttga
     3781 agacactttt ttctttccag cattatttaa aatccttaaa tagcgataat acaaaggcag
     3841 tcttttagct gtcgcctttg gaataatatg atctttcacg aaatgacctc cacgtattgt
     3901 ctaattacat ctttgatttc tccatcatag cagtatttat aggaaaaaat caattccatt
     3961 gctcagatat tgtgaaattt tattctttat tcatttattt atcatttctg caaatctcgc
     4021 agtataatgg aatttatggt agactactaa ttgaaaaata gttttttatt tgaggagaat
     4081 tcacaaagta gaaaagagga ttccatgata ttattacaag ccaatcagat tgctcgccat
     4141 tttggtgcag aagtcttatt tgaaaacata catttagaaa tcagtacagg cgctcgtgtc
     4201 gctttagttg gcagaaatgg cgctggaaaa tcgactttat taaagatcat tgctggaatt
     4261 gaagcacctg atgaaggaaa gatcgctaaa aataaaacag cgactctcgg atacctcgct
     4321 caggacacag gacttgattc caatgaaaca gtttggaacg agatgctgaa agcttttgaa
     4381 caagttagac aaatggaaga gcggatgcgc gagctagaga ttttcattag cgaaggtacg
     4441 ccagacacac ctgcttatag ttcttattta aaagaatacg accggctgca acacgatttt
     4501 tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa
     4561 ttcgattctt cattttatga tcagaaaatc gatactttat caggcggaca gcgcacacgt
     4621 ttagctttag ctcgcatgct tttgcaacac cctgacattt tgattctaga tgaaccaacg
     4681 aaccatttag atatcgagac actttcctgg cttgagaatt atctgcaaag ctatactggt
     4741 gcattattga ttgtttccca tgaccgttat tttttagata aagtagtcaa tgaagtctat
     4801 gaattaagca gaagaaaaat gcgccattac aaaggtaact attcgaaata tctagatttg
     4861 aaagcagaac agttagcaag tgaatggaag gcttatgaaa agcagcaaac agaaatccat
     4921 aaattagaag acttcgttgc tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa
     4981 agccgcagaa agacattaga aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa
     5041 caagcaaact ttctattcag tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa
     5101 gatgccgcta tcggatatga taatgtcacc ttatctgaac caatcaatct agatatccgt
     5161 cgtcaagaag cgattgctct agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa
     5221 tcaatcattc accagatccc ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc
     5281 atcggctatt acgatcaagg tcaagctgat ctgcatacaa acaagacgat cctagcagaa
     5341 ttgtgggatg agcatcctac cactccagaa aaagagatcc gaagtgcttt aggtgctttc
     5401 ttattttctg gcgaagatgt agaaaaacca atccctctgc taagtggcgg tgaaaaagca
     5461 cgggttgctt tagccaagtt agcgatgaac aaagaaaatt ttttgatttt ggatgaacca
     5521 acaaaccatt tagatattga caacaaagaa gtattagaaa atgcattgat cgattatgac
     5581 ggcacactgc tttttgtttc ccatgaccga tatttcatca atcggatcgc gacaaaagtc
     5641 gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag actatgatta ttatttagag
     5701 aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac aagatgagcc agaatctgct
     5761 cctgctccca cgaacaaatc tgattattac caaagcaaag aacagcaaaa gcttatccgt
     5821 agccttcaac gaaaaattac tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc
     5881 gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat
     5941 gagctgaacc aagatttaga agctgctaga caagatcaag aaacaaaatt gacggaatgg
     6001 gaagaactaa gcttggaatt agaagaatat gaaaattaac gaaaaaacca aaacagaaac
     6061 cattcatgct gttttggttt ttcttttttt attttatcaa acaaaaattc catatttacc
     6121 tttttcacca ataagaagac caattactag tcattctcac taaagcttct aaataatagt
     6181 aatcacccca aatacaaggt gtatttaccc cattattttt gggcttatta taaacagact
     6241 gtgttaaaaa gccatcttct tcttccgttg tagcatatct ttctttcaat tgtcgcataa
     6301 ttttccaaac agcagactgg tataagttct tttctgtttt gtctaataga cctgattcta
     6361 ttaattctaa taatccacaa accatgatca ccatactaga tgtatctttt tcttctctgc
     6421 tagaatcatc aaagatcaaa tcccaataac aaatggtatc ttctggcaac cgattcataa
     6481 aataattggc taatattttg cttaaatcaa aatatttttg ttctttcaaa taacgataac
     6541 ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc ccaacaggaa ttatcactat
     6601 aaccttgatg cgtacttcca tatcttggtt ttccatcttt gacatccata taaaatgtat
     6661 gataagttga accatcttct cttactaaat aatctgctgc attttgaata tgagcctgag
     6721 cagctttttt atatttttga tttccagttt cttcactcgc ccaatacaat aatggaagat
     6781 tcatgcaaca atccatgatc attctacctt gttgtgaagg atcactagga tctccccaag
     6841 cttgtataac tcctgccttc tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg
     6901 aagcttcctt catttttcga tttcccgtta ttttataatc tgctacggtt gataaagaat
     6961 acaaaaaacc aagatcgtga tgattagtga taatttgaca gtcaatcctg tggataaaat
     7021 ctatatctgc tttttcagct gtttgttgaa ataaaggatc acccgtcttc tcaaataaca
     7081 accaattaat tcctgtccaa aatccttctg tccaaccaat attgtctctt aatggataac
     7141 gtccttttat aggacactcg cttgggaatt gatactgact gttactgata cgtttgatta
     7201 acctctccgt gtgtctgcga caacgctcga tctcattatt ccattctttt tccattaatt
     7261 ctttataaca ttcatttatc catttttctg gatgtattat ctcttcaaac atcactttat
     7321 caagcatttc cccaccagcc tttagcataa ttttagtctt tttttattgt ttttattttg
     7381 gatagttagc ccggttgtcc catcaaaatc aattttggga cgattgttcc atacttctaa
     7441 actataatcc cgatcttgat gagcaaccac tcctgttccg agccaatggg ttccttttcc
     7501 aaatgtaccc tctaatccag gaataaaagt gttttcccat gtcatcaagt ttgtattggg
     7561 caaacccata atagctgtgg attttcgttt aatagtatcg tcacttaaac agataatacc
     7621 tgaaaagcca gcttcactca ccctatagtt ttccttgtct tcttctatga ttattccttc
     7681 tttctcagga gaacattcaa tagcaaaccc tgtctcatat agaacatatt ctgtatctaa
     7741 ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga ataagccaag ttctgatttt
     7801 tgcctgtaaa gtaggttccc atgtagaggc aagtacctct ttcttacata caatgtctcg
     7861 tacattattt cgaacgatga actgtccata ggttttgatg ctctcattct taaaactggt
     7921 cgtgatatat gtgtccgctc gctgaatccc tagcattgaa tccattgcca tttctgcttt
     7981 caattgattc gctcgcggta tcgaaaaacc aaattgacta gaataagcaa attttaaata
     8041 tttttcattg gtcaatgtat ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt
     8101 atgtccatta tcatgttgta tgagcaaatt ggcttctgaa agaagtttag ttccttcacg
     8161 aataggcata ggcatttctt tagccgtcca atactcatgg tcttcaggta gcgccaacaa
     8221 taaaaaaatc ttattcagcc ataatggaga taacgtacta ttatagggtt cagtcaatgt
     8281 caattgaggg taggtataac caatattcag gatacctttt tcatcaaata tttctttttg
     8341 catccaccat cttatattcc gattgactaa ccccttgatt tcacctaatt taaaaggcaa
     8401 aagatcggcc cacaacatag ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct
     8461 tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa
     8521 atcaatagct cggttcttat atatctggct aatgggcttt tctggttcta gtacgctata
     8581 aatcaaacca taataatgaa atgcaaaagg attataataa tcttggcgac ctcttgaact
     8641 gtctcgatac cagccatttt gaagataaca attttctata ttatcaaatg cttctttcag
     8701 tgctgattct ttaactggat aaccaaattt ttggataatt gttccgacaa ttatcttaaa
     8761 aaattgccaa ttaccatctg cacattcata ttcaaaaatt tgcgagaacc aatgaataat
     8821 ctggcttttt tctcctgtat ctaatttttt ccataataat tgttcatgat gatataacgc
     8881 taataacatt gcgggcattt ctaccatacg ctggtctctt tcatgtattt ctccccaata
     8941 atcgggatgg gaaggatctg ttccagcttt tattcctgat agaattaatt tcaaccatcg
     9001 ttcatctaat tggtcaattg cgggccccgc tccccataaa acccgagaga atccttccat
     9061 tcctgcaatt ttatttccat atcctacccc ttgatgtata taatcaattt gtgcgccact
     9121 tgctcgataa aacggctgga gtggttgatt tatcttttta aataagtcaa agtaatcctg
     9181 tctggtcctt aatagtggta ctggatgttt cattgtattt tctcctcttt ttactaccaa
     9241 atagctaatt ccgactgttc taatcgaagt aagccttcaa taaaaaaata atcagaatag
     9301 atcagtggca cattataagt cagttctcta tggtatgctt ctgctccgcc tccaatgatc
     9361 ccatcttcat ccatattcca atttgcatag tgcttttctg tcttttggag tatattgata
     9421 gcagcttcat gatacatttt tgatcgtaca gaggaaacat atcctgctaa ttccagaaga
     9481 ccgcatgcag cgcatactcc agcactggta tcaatattcg aatcttttac agcaaaatca
     9541 atgagaggaa tataaccagt tttttcgatt ttctgaataa aatttactgc tatcttttct
     9601 gctatagcta gatagtacgg attttttgtg taacgataac ttaacgcgaa tccatataaa
     9661 gcccatgcat ttcctctgct ccaacaagaa ttctcattgt atccttgccc gccaagtaac
     9721 cgaatatatt tccctgattc tgcatcaaat tctgcgatat gcccggttga tccatctttc
     9781 ctcactagat acttagctgt tgtatctgca tgctgccgag caattttaga gaacctcgga
     9841 tcaccagttt ccctcgaagc ccaatacagt aatggtaaat tcatcatact atcaataatc
     9901 atccaaccag gtttatggtc attccatgct actaaatagt tccctgaaat attatagcgt
     9961 ccagcaagta ccgtagctgc atgtaaccct agttgtcgac tatgtttgtt ttgagactgt
    10021 tgataatgtg caatagccga tggtaagaat aaaaaaccta catcatgatg aacgtctgaa
    10081 aaatggtaca gggcagattc taataattgt tcttgttgta ttgctgcctc tttgaacttt
    10141 tctcgttttg tcttgtgaaa gagctgccat aaaataccac tccaaaaacc attggtccac
    10201 cagtcgattc ctctgtcctt tattacgtct gtatattttc cgttgtaaga aatatatgga
    10261 attttattcc ccattctgtc taattccgca tctatctttt cagaagctac attcaaaatt
    10321 tttcttgacc attccttcgt ctgttcattc atggatatcc cgccattctt ttaattttta
    10381 tgaatgtgga gttgctccta gaacgaacta gatggttaac attcgttatt ttttttactt
    10441 atttgcagct ccaatgtcat tctccttaaa tttagactgc gcccacacat aaatcaagac
    10501 cattgggatc actgaagttg ctaaaattag cataattgcg ccataattaa ttggctcacc
    10561 tgtactagaa accgtcatat ttttgataat taagggaacc gtatatttat ctttatctga
    10621 aagcatgact aatggaaata gatattgatt ccaacttccc ataaagataa gtaaaccact
    10681 agtaataatc gtaggtgata aaatcggtac agcgattttc gtaaaaatca cccactcatt
    10741 ggcaccatcg attctggcag cctccatcag tgctgtagga aattgactgc tatattgtcg
    10801 catcataaac actgaagttg tactggcaat cgaagggatg atcacacctg cataagtatt
    10861 cgataatcct aaacgactca taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac
    10921 catcctagat agcataactg tattgaacaa cagtttattc cccataaaac gatactttgc
    10981 taatgcataa cccgccattg tagaactggc tgtagctaaa actgtactga ttacagcaat
    11041 aacaatcgaa ttccaaataa tcaagggata attatatctc gccatgatgg cttgccagtt
    11101 ttttaatagc atatccccaa acttgaaatt gattccgcca cttaaaattt cttgattact
    11161 ttgcgttgaa gctacaatca tatataaata tggcatcaaa aatattaagg caccaaacac
    11221 aataatgacg tacgtaaaaa ttctgctttg tatcttttgt ttagtcattt ttatcacctc
    11281 cgaatttaaa ttgaatagcc gataatataa cagatacaaa caccatcgtc caggcaatag
    11341 cagaggcttt tccaaaatca accatttcaa aacttgtttt atataaattg actcctacga
    11401 ccaaagctga attttctggt ccattggaag tcccaaagaa tatatttggg atattgaaat
    11461 tacccaagcc attgatagtg gctaaaactg tggccattaa aatgattggc tttagcatgg
    11521 ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc atctatttca gcactttcgt
    11581 atatctcttt agaaatccct tgaagaccag ctaaaaataa aatagtgtta tatcctacac
    11641 taagccaaat catcgtgatg atcattgata ttcgtatcca aaaaggattt gttaaccact
    11701 gtacaccgtt aattcctagc gcattcaaaa atccattgaa taacccagtc ggattgaaaa
    11761 aagtcatgaa tacggaagct acagccaccg ttgatgtaat ggttggaata taatagacag
    11821 ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa taaaacagca aagtaaatag
    11881 ctaatagcat cgttattgga attgatcctg ccatgataat caaaacattc gtgtaagatt
    11941 taataaacaa tggatctcgg aagatagcta gataattgtc aaatccaaca aattcaaatg
    12001 agtagccaat tttcataaaa ctaaaagcaa acgagataat gatgggaatc aaaatcgtca
    12061 caataaagaa aataacaaaa ggtgtgacca atccataagc acttttttta tgagacgtat
    12121 ctgttgcttt tttcttgctg ttctttattg tcttgcttgg taacgaagat tgcatcatcg
    12181 ttaagtcctc ctaatatttt ttaatcacca gttaatttta cgccgtactt atgagcaaaa
    12241 ttttctgctt cttttagata tgaattttca aaatcacctg tttgccaata gttatatgca
    12301 gcggtagtca aataagacgt agcatctgaa taagtggctg gtaaaacagt atttgatgta
    12361 ttttcattga ttttattcaa tagttcatag tatttttgtc ctccttagta gtcaaacttt
    12421 ttctcagctg cttctgtttg ataggccaat gagttagatg tagccacgcc cagctccagt
    12481 gcgttggcta agcattcttc atctgtcagc ataaatgtaa gaaattgttg tgccacttgt
    12541 ggatttttgg catctttagg gacataaaaa cttgatcctc ctgatactgc accttttcct
    12601 ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc atttgtcaga tgcatcagga
    12661 aaattcaaag acatattcgt agcataccag ccgccagcaa caaacattgc agattcttga
    12721 aatgctttct cagcatcttg tgcaccgtag aaatgtacaa tatctgagtc gatcatttct
    12781 ttgattaact ttgcagcatg tttcgcttct ttagtattca gacggatatt tccttcttca
    12841 tcaagcaaag attgattctg ctgggctagc atgttgataa acaattcttg gtccccagat
    12901 tctggcaagg caattccaaa actaccagtt ttttctttta atgcttttgc cacctggata
    12961 tattcttccc aagaactgat ttcttcaaaa tctacaccta tttcagcaaa tttttttggt
    13021 tgataataac aaagtgctgg ggtaaaatca ctagcccaag gaataatcgt ttcattactc
    13081 gtttgttgac gcaaaatatt caatttttga tcataaaact gatcgccata tttgtcataa
    13141 aatccatatt catcagcact ataaaaagca tcttgaaatt tttccagcac acctgctgct
    13201 gaagtgtctg ccatggagat aatatccggc atatcttcgt gtgttaccaa ttttggggtg
    13261 atcatttgta ctgttgctgt tcccgaagct acattcacaa tttttaagtc aatatttgct
    13321 ccagttttgc ggttatagat ttttgcagca ttttcaacat agtttgtcag tttgccgttt
    13381 gcgacatata cttctaatgt agtggctttt tttatcattg gatctgcgat gtttgctgtt
    13441 acttcttcgt tagtttttcc acatgcactt aagctgaata atagggtaat gtttagcaaa
    13501 gctatcacca atttgtatct ttttttcatg tctatccctt ctttcttttc aagttcttat
    13561 ataaaacgct ttcacttatg tatattaacg gaattagtaa cacaa
//
LOCUS       DAHUWM010000069        13590 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHUWM010000069 DAHUWM010000000
VERSION     DAHUWM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.069"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(18..1241)
                     /locus_tag="NK788_001374"
     CDS             complement(18..1241)
                     /locus_tag="NK788_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCH6769712.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(1272..2111)
                     /locus_tag="NK788_001375"
     CDS             complement(1272..2111)
                     /locus_tag="NK788_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCH6769713.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(2128..2808)
                     /locus_tag="NK788_001376"
     CDS             complement(2128..2808)
                     /locus_tag="NK788_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769714.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(2810..3883)
                     /locus_tag="NK788_001377"
     CDS             complement(2810..3883)
                     /locus_tag="NK788_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6769715.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(3939..4177)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(4220..4453)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4530..5201)
                     /locus_tag="NK788_001378"
     CDS             complement(4530..5201)
                     /locus_tag="NK788_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6769716.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(5288..7966)
                     /gene="mgtA"
                     /locus_tag="NK788_001379"
     CDS             complement(5288..7966)
                     /gene="mgtA"
                     /locus_tag="NK788_001379"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCH6769717.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            8625..9062
                     /gene="ndk"
                     /locus_tag="NK788_001380"
     CDS             8625..9062
                     /gene="ndk"
                     /locus_tag="NK788_001380"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCH6769718.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(9084..9950)
                     /locus_tag="NK788_001381"
     CDS             complement(9084..9950)
                     /locus_tag="NK788_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6769719.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(10110..12203)
                     /locus_tag="NK788_001382"
     CDS             complement(10110..12203)
                     /locus_tag="NK788_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCH6769720.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(12952..13575)
                     /locus_tag="NK788_001383"
     CDS             complement(12952..13575)
                     /locus_tag="NK788_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769721.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         3856 a         2907 c         2325 g         4502 t
ORIGIN      
        1 cacaagttta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct
       61 tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta
      121 ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca
      181 gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg
      241 attgttggta tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt
      301 tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca
      361 tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa
      421 gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta
      481 tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg
      541 acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat
      601 tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact
      661 cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga
      721 tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct
      781 acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg
      841 cccccttctt cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat
      901 tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca
      961 tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta
     1021 ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa
     1081 aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt
     1141 acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg
     1201 attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct
     1261 agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat
     1321 gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac
     1381 gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg
     1441 tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct
     1501 acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca
     1561 tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct
     1621 tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg
     1681 ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca
     1741 tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact
     1801 aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc
     1861 aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg
     1921 tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta
     1981 tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt
     2041 ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata
     2101 atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact
     2161 gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc
     2221 tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac
     2281 tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat
     2341 gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc
     2401 catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg
     2461 acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat
     2521 cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg
     2581 gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag
     2641 taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc
     2701 aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc
     2761 cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc
     2821 cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac
     2881 cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa
     2941 cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg
     3001 ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa
     3061 tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa
     3121 tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt
     3181 cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt
     3241 ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga
     3301 cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg
     3361 ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca
     3421 aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag
     3481 atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct
     3541 gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt
     3601 ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt
     3661 tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct
     3721 gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc
     3781 cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt
     3841 ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc
     3901 taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg
     3961 ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc
     4021 tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg
     4081 atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa
     4141 ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta
     4201 ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt
     4261 ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa
     4321 cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct
     4381 tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt
     4441 tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa
     4501 gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt taacatttca
     4561 ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg
     4621 gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa
     4681 gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct
     4741 gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct
     4801 ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct
     4861 cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc
     4921 aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa
     4981 tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact
     5041 aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg
     5101 atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg
     5161 tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc
     5221 aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc
     5281 ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa
     5341 gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca
     5401 tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca ataattccta
     5461 atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg
     5521 tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat
     5581 ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca
     5641 ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac
     5701 ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct
     5761 cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga
     5821 tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg
     5881 caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca
     5941 tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt
     6001 tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa
     6061 taccgatatc agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga
     6121 cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt
     6181 ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga
     6241 ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg
     6301 taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt
     6361 tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa
     6421 ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg
     6481 cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt
     6541 cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt
     6601 tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct ggatcagcaa
     6661 ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat
     6721 tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat
     6781 ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt
     6841 catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg
     6901 ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga
     6961 tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca
     7021 ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta
     7081 gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa
     7141 atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt
     7201 tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga
     7261 ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg
     7321 tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc
     7381 ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg
     7441 gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa
     7501 tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt
     7561 tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa
     7621 ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat
     7681 attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt
     7741 atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac
     7801 cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga
     7861 atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat
     7921 ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct
     7981 tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga
     8041 tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt
     8101 gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt
     8161 tatttttcca attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa
     8221 aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct
     8281 cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc
     8341 taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt
     8401 ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc
     8461 cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac
     8521 tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc
     8581 acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg
     8641 ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg
     8701 aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa
     8761 aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga
     8821 cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta
     8881 agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg
     8941 cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag
     9001 agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt
     9061 gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc
     9121 tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg
     9181 tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc
     9241 atcaatgttt catagatcac tgtacttctt tctttatgtg ctaagctccg tttcatatct
     9301 tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc
     9361 attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt
     9421 tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca
     9481 gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg
     9541 attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc
     9601 atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta
     9661 ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt
     9721 tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt
     9781 gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa
     9841 tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt
     9901 gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc
     9961 ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac
    10021 gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt
    10081 gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct
    10141 gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga aaagacactt
    10201 gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag
    10261 tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt
    10321 tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag
    10381 atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa
    10441 gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact
    10501 acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg
    10561 attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg
    10621 actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg
    10681 ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta
    10741 tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata
    10801 ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt
    10861 cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg
    10921 tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt
    10981 tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa
    11041 cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag
    11101 tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt
    11161 gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca
    11221 agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca
    11281 gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct
    11341 tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta
    11401 atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc
    11461 ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca
    11521 atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca
    11581 taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc
    11641 tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct
    11701 gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt
    11761 ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg
    11821 acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa
    11881 aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc
    11941 aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt
    12001 ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc
    12061 cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca
    12121 atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg
    12181 cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt
    12241 tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt
    12301 ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac
    12361 taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt
    12421 tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc
    12481 ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct
    12541 cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt
    12601 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    12661 ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa
    12721 agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa
    12781 attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    12841 aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
    12901 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc
    12961 ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt gattttcttc
    13021 acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg tcgactcgtt
    13081 tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc attccgctaa
    13141 acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca cttttccatt
    13201 tttcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc
    13261 tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat taataaattg
    13321 attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt aattggcagt
    13381 tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat ctactgattc
    13441 tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga
    13501 aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg
    13561 tctttttttc ctcatgcttt cctccactcc
//
LOCUS       DAHUWM010000070        13399 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHUWM010000070 DAHUWM010000000
VERSION     DAHUWM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.070"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(15..707)
                     /locus_tag="NK788_001384"
     CDS             complement(15..707)
                     /locus_tag="NK788_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6769722.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="NK788_001385"
     CDS             complement(1144..1713)
                     /locus_tag="NK788_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCH6769723.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="NK788_001386"
     CDS             complement(1787..2110)
                     /locus_tag="NK788_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769724.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="NK788_001387"
     CDS             complement(2264..3076)
                     /locus_tag="NK788_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769725.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="NK788_001388"
     CDS             3430..4278
                     /locus_tag="NK788_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCH6769726.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="NK788_001389"
     CDS             4423..5364
                     /locus_tag="NK788_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCH6769727.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="NK788_001390"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="NK788_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="NK788_001391"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="NK788_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCH6769728.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="NK788_001392"
     CDS             complement(9828..12014)
                     /locus_tag="NK788_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCH6769729.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="NK788_001393"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="NK788_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCH6769730.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="NK788_001394"
     CDS             complement(12236..12676)
                     /locus_tag="NK788_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCH6769731.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="NK788_001395"
     CDS             complement(12750..13289)
                     /locus_tag="NK788_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6769732.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DAHUWM010000071        13343 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHUWM010000071 DAHUWM010000000
VERSION     DAHUWM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.071"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            15..161
                     /locus_tag="NK788_001396"
     CDS             15..161
                     /locus_tag="NK788_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCH6769733.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(223..2034)
                     /locus_tag="NK788_001397"
     CDS             complement(223..2034)
                     /locus_tag="NK788_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCH6769734.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2031..2396)
                     /locus_tag="NK788_001398"
     CDS             complement(2031..2396)
                     /locus_tag="NK788_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCH6769735.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2559..2954)
                     /locus_tag="NK788_001399"
     CDS             complement(2559..2954)
                     /locus_tag="NK788_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCH6769736.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2972..3934)
                     /locus_tag="NK788_001400"
     CDS             complement(2972..3934)
                     /locus_tag="NK788_001400"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCH6769737.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3934..4146)
                     /locus_tag="NK788_001401"
     CDS             complement(3934..4146)
                     /locus_tag="NK788_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCH6769738.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4167..4865)
                     /locus_tag="NK788_001402"
     CDS             complement(4167..4865)
                     /locus_tag="NK788_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCH6769739.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4885..5427)
                     /locus_tag="NK788_001403"
     CDS             complement(4885..5427)
                     /locus_tag="NK788_001403"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCH6769740.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5559..6563)
                     /locus_tag="NK788_001404"
     CDS             complement(5559..6563)
                     /locus_tag="NK788_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCH6769741.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6560..7549)
                     /locus_tag="NK788_001405"
     CDS             complement(6560..7549)
                     /locus_tag="NK788_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCH6769742.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7546..8352)
                     /locus_tag="NK788_001406"
     CDS             complement(7546..8352)
                     /locus_tag="NK788_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769743.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8518..9474
                     /locus_tag="NK788_001407"
     CDS             8518..9474
                     /locus_tag="NK788_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6769744.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9551..10069)
                     /locus_tag="NK788_001408"
     CDS             complement(9551..10069)
                     /locus_tag="NK788_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCH6769745.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="NK788_001409"
     CDS             complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="NK788_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCH6769746.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10534..10980
                     /locus_tag="NK788_001410"
     CDS             10534..10980
                     /locus_tag="NK788_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCH6769747.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="NK788_001411"
     CDS             complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="NK788_001411"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCH6769748.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4152 a         2831 c         2276 g         4084 t
ORIGIN      
        1 aatttgtatc tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg
       61 ttgcttctgt gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag
      121 aaaaccgtaa aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc
      181 atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat
      241 aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag gtctagctac
      301 tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg
      361 gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga
      421 atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa
      481 attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat
      541 caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg
      601 ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa
      661 ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca
      721 tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg
      781 gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt
      841 gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg
      901 ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag
      961 tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt
     1021 tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac
     1081 gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg
     1141 ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt
     1201 tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt
     1261 gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac
     1321 tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc
     1381 tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa
     1441 aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca
     1501 gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag
     1561 ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc
     1621 tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg
     1681 agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt
     1741 ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg
     1801 tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg
     1861 agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc gacctccagg
     1921 accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat
     1981 cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat
     2041 gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca
     2101 ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct
     2161 tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat
     2221 cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac
     2281 cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt
     2341 attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt
     2401 gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa
     2461 aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga
     2521 ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt
     2581 tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta
     2641 cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc
     2701 tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca
     2761 cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt
     2821 ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact
     2881 cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt
     2941 acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag
     3001 caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta
     3061 cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg
     3121 ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc
     3181 cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt
     3241 cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg
     3301 ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg cgtaccacac
     3361 ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt
     3421 caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg tgaatcaaat
     3481 ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat acaacgtctg
     3541 gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc gcgtcattat
     3601 cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat tgaacttctg
     3661 tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg cttcctgggg
     3721 tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc aaacgatgat
     3781 tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata ttggtatcga
     3841 tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt gctgttgttc
     3901 cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct ttcggctttc
     3961 ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat catgatcaat
     4021 caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt catagatgcg
     4081 tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat catataagct
     4141 ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt taaacccagt
     4201 aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct ttggtgtatc
     4261 gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa tccgccaatt
     4321 agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa aacaaatgtc
     4381 aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt atgtttaaaa
     4441 tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact tgcaccacca
     4501 gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat cccgctgaac
     4561 atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg ccccataaaa
     4621 tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa gattggtgtc
     4681 actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc ggcataataa
     4741 gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg tatccctaaa
     4801 aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg ctgttgataa
     4861 ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct tctgatcaaa
     4921 gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac atgttttttt
     4981 atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc gataaccggc
     5041 tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca aatagagctt
     5101 ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact gttcatccat
     5161 ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt tcttttgttc
     5221 tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg ataaagtcgt
     5281 ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat agattcgctg
     5341 ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga ttcctttccg
     5401 gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat aaaatggtat
     5461 aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag aataaaaaga
     5521 agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag cgcgtgtttt
     5581 tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat caaaattcct
     5641 gtaggtactt ccccacttgg tagaatcact ctagcaacca tatctgcact aagcagaaaa
     5701 atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg tacgactagc
     5761 ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac cgctacacta
     5821 ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc cacgttaagg
     5881 cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg tgcataaagt
     5941 cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata aggccagctc
     6001 gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc cttagataat
     6061 tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat accggacaat
     6121 aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata gacacagcaa
     6181 gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag cgaatagtgc
     6241 gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat gcccaatata
     6301 ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga aacgccaaga
     6361 caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct aacaatcagc
     6421 gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa
     6481 ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag caagaaatag
     6541 aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt aaaaacggaa
     6601 cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg atcatcctcg
     6661 ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca ggtagaacat
     6721 aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc ccaataaatg
     6781 aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga cagaaaacaa
     6841 tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa
     6901 tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata tataaaggaa
     6961 acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac caaaaagcaa
     7021 gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta aaaaaagaac
     7081 taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc gaaaactgca
     7141 aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt agaaacacag
     7201 aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac gaagcacccg
     7261 cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcacccct tgcatcaatg
     7321 caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa
     7381 tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac gcctgccaaa
     7441 aatctgcttt ttccacagtc gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa
     7501 gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata attgatcctc
     7561 ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc ttggaagcgt
     7621 gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga tcacttcttc
     7681 aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat ctgaaaaacg
     7741 tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca tttgattgat
     7801 ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc
     7861 atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga cacgctgggt
     7921 ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac ctgtagcatc
     7981 caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca gtttttgata
     8041 agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt gttcactagt
     8101 ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag agatatcaga
     8161 gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac ttttcaataa
     8221 agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt caggaatcgt
     8281 aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt cagctgctct
     8341 taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg acgctgtttc
     8401 aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg acagcgttcg
     8461 aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag gaaagaaatg
     8521 aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg agtactgagc
     8581 gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac gacttcatcc
     8641 aatagtactc atacagtcac agatacactt ggacataaag tagagattcc aaacgcgccc
     8701 aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga aaaacctatc
     8761 gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga tttaaaggat
     8821 gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga gcctgatttg
     8881 ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc
     8941 gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca actaaaagat
     9001 gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa
     9061 tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg
     9121 ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc
     9181 gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc
     9241 acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc
     9301 atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta
     9361 aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac
     9421 ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa
     9481 ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga
     9541 cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa
     9601 aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga
     9661 agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg
     9721 ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa
     9781 catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta
     9841 cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat
     9901 aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat
     9961 ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat
    10021 ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc
    10081 cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt
    10141 gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc
    10201 aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt
    10261 tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca
    10321 tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct
    10381 agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg
    10441 ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat
    10501 agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct
    10561 gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca
    10621 ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa
    10681 aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca
    10741 agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg
    10801 atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc
    10861 aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa
    10921 gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga
    10981 acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg
    11041 gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt
    11101 ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg
    11161 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    11221 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    11281 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    11341 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    11401 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    11461 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa
    11521 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    11581 acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc
    11641 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    11701 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac
    11761 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    11821 ggagcaagtg attcatataa atggatccgt ccatggaagt ttttcatttt tccgatttta
    11881 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    11941 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    12001 ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa
    12061 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    12121 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt
    12181 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    12241 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    12301 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    12361 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    12421 tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg caaacgatag
    12481 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    12541 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    12601 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    12661 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    12721 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    12781 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    12841 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    12901 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    12961 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    13021 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    13081 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    13141 cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg
    13201 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc
    13261 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    13321 aagacacgac ctaaagatcg tgt
//
LOCUS       DAHUWM010000072        13272 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHUWM010000072 DAHUWM010000000
VERSION     DAHUWM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.072"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(12..1274)
                     /locus_tag="NK788_001412"
     CDS             complement(12..1274)
                     /locus_tag="NK788_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769749.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1267..2166)
                     /locus_tag="NK788_001413"
     CDS             complement(1267..2166)
                     /locus_tag="NK788_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769750.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2311..4089)
                     /gene="uvrC"
                     /locus_tag="NK788_001414"
     CDS             complement(2311..4089)
                     /gene="uvrC"
                     /locus_tag="NK788_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCH6769751.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4271..4585)
                     /gene="trxA"
                     /locus_tag="NK788_001415"
     CDS             complement(4271..4585)
                     /gene="trxA"
                     /locus_tag="NK788_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCH6769752.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4672..7032)
                     /locus_tag="NK788_001416"
     CDS             complement(4672..7032)
                     /locus_tag="NK788_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCH6769753.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7164..7694)
                     /locus_tag="NK788_001417"
     CDS             complement(7164..7694)
                     /locus_tag="NK788_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCH6769754.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            complement(7752..8189)
                     /gene="zapA"
                     /locus_tag="NK788_001418"
     CDS             complement(7752..8189)
                     /gene="zapA"
                     /locus_tag="NK788_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCH6769755.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8391..9308
                     /locus_tag="NK788_001419"
     CDS             8391..9308
                     /locus_tag="NK788_001419"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCH6769756.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9564..10475)
                     /locus_tag="NK788_001420"
     CDS             complement(9564..10475)
                     /locus_tag="NK788_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6769757.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10878..11456)
                     /locus_tag="NK788_001421"
     CDS             complement(10878..11456)
                     /locus_tag="NK788_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6769758.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11476..12144)
                     /locus_tag="NK788_001422"
     CDS             complement(11476..12144)
                     /locus_tag="NK788_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769759.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12159..13229)
                     /locus_tag="NK788_001423"
     CDS             complement(12159..13229)
                     /locus_tag="NK788_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769760.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         3766 a         2853 c         2188 g         4465 t
ORIGIN      
        1 ttttctatat tttatgcttt ttttcgttca tttctcataa ttgaaatcga ttgtttcaat
       61 gaactccata atccgccttc gctatacacg agtacattcg atttgtacat gtttgcagac
      121 aagatcatca ataagaatgt tacggctaca agtaccaaca aggaaatcaa tgctccaccc
      181 ataccgactg tctcatttgc taagcgaacc ggcataatat atgaagataa gaatggaata
      241 tacgaagtga tccgaataac gatattgttt ggatctgaag ctcctagtat caatccaagc
      301 atatatccgc ctagagatag ataagtcact ggcaaaattg cttttgctgt atcttctgct
      361 ttgttgacca aagagccaca taaagctgct aaaacagaaa aaatcaaaat gccaaagatc
      421 atgaatagaa gagaatacca caagaatggt ccaatcaagt tatccaatga gataccgtct
      481 aatatatttt tgaccatttc catatctttg aattgactgt atcctatacc aaaaataata
      541 ccgtataatg ccatttgcgt taatgcaaca agtaaaacac ctgttagttt tccgtaaaaa
      601 tgcttttgtg cagtcgtact ggacaggatc acttccatga tccgcgtgcc tttttcagaa
      661 gcgatctctt gtgcaatgat ctgcgcatac gtcaaaataa taatgaacaa gacaatcgtt
      721 gctacataac ttacgacata ttggactgtc gagttatctt ctccaactgt catttttcct
      781 tttgaatcaa aattgacttt ttgtcgagtc aaacttgctt gttggctcaa gctagctact
      841 tcttctggtg aaatgcctaa ttgacttgct cgaagcatcg attgcaagcc agtcagttgt
      901 tgttgtatca gtagttgtgt actttgtcct aatgaatttt cgctataaag catcccattt
      961 atcttatcat tctcagttga gactactaaa tacgcatcga ttttttcttc cgaaagctct
     1021 ttttgtgctt tttcttcaga atcaactgct ttgaactcaa aatcatctgt tttgacttgg
     1081 ctcatttgct gactgatttg ctcattatca gccaatacgc cgattttatt gacatcacta
     1141 ttttgttgag caaagtttcc tgctacataa atgataccca gcatgataaa agggactaat
     1201 atcatgatca agaaagaaat cgacttaaca ttttttaaat agacatcttt ggtaataatc
     1261 caaaacttac gcatcgattt cacctgcttt caatttgaaa atctcttcca aagttggcgg
     1321 ttgctggtta aacataggaa tgtagccgaa ctgagtcgca cgagtaaaga tttctttccc
     1381 agcttcagga tcttctaagg taatctctaa cgtattgtct tcatgaagga tcactttttt
     1441 cacgccttcg atttcttcta cttcctgttg gcttaaacca gattccaaga ataacttcgt
     1501 acggccaaat gattcacgaa tctcatgtac ttttccattc aaaaccattt ttccgtttcg
     1561 caacatgatc aaatgatcgc agattttttc tacattatcc atattgtggc tagaaaaaat
     1621 gacacatgaa ccatgttttt tcaattccaa tattccatct tttagcaatt cagcatttac
     1681 cggatctaac ccgctgaatg gctcatccaa aatgatcaat ttaggttcat ggattaacgt
     1741 ggcgatcagc tggacttttt gctgatttcc tttagacaaa gattttactt tatccgtctt
     1801 tttccctttt acctgaaatt tttccatcca aaaatcgatt tttggttcaa tctcttttct
     1861 tgatttccct ctaagttctg caaaataaag cagttggtct tgaatcgtta ctttcggata
     1921 aagtccccgt tcctccggaa gataaccgat gacatcataa tctttctccc caagatgatg
     1981 gccattccat aacacttctc cttggtcttg tgtcaaaaaa tccaagatca aacgaaaagt
     2041 cgttgtcttc ccagccccgt tttggccaat tagccccatg atttttccat cggggatttg
     2101 gaaagagaca tgatcgacag ctgtgtagtt cccaaaagtt ttaacaagat ccttgacttc
     2161 taacatttct tcactccttc ttcaagaaat cacatatatc cttagacacc agacgtaagc
     2221 gatttattat ttaccaacaa aacgtaaacg ctctttttgt tgtacataaa atgataggac
     2281 actttacttt ctttgtaaag tgccctttcc tcattcctga tgtaatttat caaatacgtt
     2341 ttgtgcgaca tttttcggca gtccgatttc ttgtaattct tctgtcgtgg ctgctgtgat
     2401 atttttcaac gatttgaact ctttaagcag catttttttt cgttttggtc ctaacccttc
     2461 aatttcatct aatcgggaag caaaactgtt tttactgcgt agctgccgat ggaaagtgat
     2521 tgcgaatcga tggacttcat cttggattcg ctggagtaag aaaaactctg gtgaatttcg
     2581 ctcaagcggt actacagcca aatctatacc gaatagcaac tcatttgttt tatgtttatc
     2641 gttttttgcc atgccagcaa taggaatatc gattcctaat tgattttcta agacatcttt
     2701 tgccacatct acttgtcctt ttcctccatc gatcaagatc aaatcaggta aaggcaaccc
     2761 ttcgcgaatc actcgtgaat atctgcgata gatgacttct ctcattgatg cataatcatc
     2821 cggtccgttg actgttttta ttttatattt ccgatattct tttcttgccg gttttccatc
     2881 aatgaatacg accatcgcag ccacaggatt cgtccccatg atattagagt tatcaaaaga
     2941 ctcgatacgt attggggtcg ggatattcat cgcgtttcct aaacgttcaa ctgctccgat
     3001 cgtccgttcc tgttttctgg caatcagatc aaaccgttca ttcaaagcaa ctgaggcatt
     3061 cttattcgcc aattcaacta attttttctt ttctcctcgt tttggttgca gcactttcgt
     3121 agctaacaaa gcttctacac tgggcttgtc gatcgtatca ggaatcaaga cttctttagg
     3181 aatgaaatgc tcattttcct gataaaactg accgaggtaa gtcaagaaat cttcttctgc
     3241 ttcgttatag aaaggaaaca ttgaaacatc tcgttcaatt agctttcctt gtcttacgaa
     3301 aaacacttgg acacacatcc atcctttgtc aatcgcataa ccaaatacat ctcgatccag
     3361 taaatcagta tttgtcattt tttggcgagt catgattgtc tcaatggcac ggatctggtc
     3421 tcggtattct gcagcttttt caaactccat gttttcagca gctttttcca ttttctgatg
     3481 gatctctgac tcgatttttt cgtgtcctcc gtttaaaaac tttttcactt catcaacgat
     3541 tttcgtatag actttcggat ctacgtcgaa ataataagga cacagacatt gtcccaaatg
     3601 atagtaaaga caaggctcct tcgtctgact aggtttgcat tttctcagag gaaataatcg
     3661 atcgaggatc ttctttgtct cattcgccgc accgacatcg ggatatggtc caaaatacag
     3721 tgctttgtct tttagtactt tacgagtaat caccaaacgg ggatattttt cattcgttat
     3781 cttaaggaaa ggataggttt tatcatcctt taacataatg ttgtatttag gatcattttt
     3841 ctttatcaga ttgatctcta agagcaatgc ttcgatattc gattctgtaa cgatgtattc
     3901 aaagtcttcg atttcactaa caagccgttc cgttttcgta ttatggctgc cagtaaaata
     3961 tgagcggaca cgatttttta atattttggc tttgccgaca taaatgatcg ttccattttt
     4021 atctttcatc aagtaacagc cgggctgatc aggaagtaaa gctaatttgt ttttgattcg
     4081 ttcgttcatc tggtactccc cttcatattc aattaaagtt attatagcat gtctgtcaaa
     4141 aaaacaatcc agcctgacaa cgtaattata ctcaaaaaat attaatttga ataaccttaa
     4201 ctcaaccgca aaaaaaccga ggacggaact ggattcagtc ccaccctcag cttatttttg
     4261 ctgtaagttt ttataaatat tgatcgatca tttgacgtag ttgttctttt gaatgaacac
     4321 caacagcttt ttctactact tgaccatctt ttttcaacat taatgttgga atgctcatga
     4381 taccgaattg ttgaggagtt tctggatttt catccacatc cattttagca attctgaatt
     4441 cttcttcatc atattcttgt tccaactgat ccaaaatcgg tgcttgcata cgacatggac
     4501 cacaccaagt tgcccaaaaa tcaatcaata caagtccttt atctgtttct tcattaaatg
     4561 tcttatctgt aattacttgt gacatatcaa agacctccat ttttaaaatc tatgattagt
     4621 atagcatttc tatacttgga tagcgaacca tctgcttacc tttagtaagc tttacttaaa
     4681 tttaacaatt gtcgcaccat ttccgccttg gtttgccggt gcaaactcaa agcttttgac
     4741 gctccgatgg tttttaagat aatcagtaat tcccttgcga agtgcgcctg ttccttttcc
     4801 gtggacaata gtcacttgag gataaccagc tagaatggca gaatccaaat attgatctac
     4861 ctcacttagc gcttcttcgt atcgttttcc tcgaaggtcc aattgattcg gcacgtgact
     4921 actagattca gatcgtacag catggataac tcgttgtgtc ggctcttttt gaggagcaac
     4981 aggcgtcagc tcgtcttcgg acacattcat ttttaatata ccgatttcta cttgccactg
     5041 attcccgttt ttacgtaaaa gcgtccctct ttggccgtaa gtcgtaacca acacttcgtc
     5101 tcccggtttc aacgtttttt gttctttcgc cttcttcaat actttatttt tcttcaaagt
     5161 ttcttcttga tgtagatttg ctaattgggt ctttgcatcg atcaactgat gctctttcac
     5221 atttccttgc ccaatctgct gttgcatttt acgaatatca gcaatgattt tttctgcttt
     5281 ttcttccgcc tctgacacga cttcgtttgc ttttttcttt gctttttcca tttctttttc
     5341 acgttcttca aagaaatagc tgtaagcttc tttcaaatca tcgtgaagtt cttgtgcctc
     5401 agaaacaaaa tgacgcattt ccagatattc ggtctctgcc attttccggc gattctctaa
     5461 atctgtgatc atttcgttta aatcttggct ctcatcattc atcaactgct tcgcttcatc
     5521 aataacttct gtatcaagac ctaatcggct agagatttca aacgcattgc ttcgtccagg
     5581 aacgccaatc agcaaacgat acgttggact tagtgtatcc acatcgaact ccatgctggc
     5641 attaatcgta tttgcccgat tgtatccata aactttcaat tcaggataat gcgttgttgc
     5701 catcacataa gcagactttt taccaagatc atctaagatt gcgattgcta gagcagcacc
     5761 ttcttgcgga tctgttcctg caccgagctc gtcaaataat accagacttt tttcattgac
     5821 ttttgataaa atatctacag tgttcgtcat atgagaagaa aaggtcgata gactttgctc
     5881 aatcgactgt tcgtctccta tatccgcaaa tacttcctca aaaataccga tttgactttc
     5941 ttcacctgca ggaatcggta atcctgcttg ccccatcagt tgtaacagac ctaaagtctt
     6001 caacgtgatc gttttcccac ctgtgttagg tcctgtaatg acgatggctt gatagtcttt
     6061 tccgattgtg atgtcattag aaactacttt ttcctgatcg atcagtggat gtcttgcttg
     6121 tttcaagacg acatgattgt cttcactgat tcccggcacg atggctttta cttctttacc
     6181 aaatcgtgct tttgcattca tcagatccat tttaccaatc acataagcat tgtggatgat
     6241 ttcttgacga tggggaacaa gttcagcaga aagttcactt aaaatacgtt gaatctcatt
     6301 tcgttcagcg atttggtatt gacgcaaacg attattcaaa tccaccactt gttttggttc
     6361 aataaataaa gtctgtcctg aagcactttg atcgtggact acaccgccaa atacaccgcg
     6421 gtattcttgt ttgactggga taacgtagcg gtcatttcgc atggtgatga tcgcatcact
     6481 taaatacttt gcatttttcc cacggacgat cccatctaac tgttcacgaa cagcttgttc
     6541 acttcgccga atgttctgtc ttatgctctt taactcagga gaagcgtcat cagtcacgcg
     6601 tccgtcttca tcgattgctt cctttaaacg gcggctcaaa acagggatcg tgactaactg
     6661 atctgcccac atatacaaac gtgcaaattc gatttcactg tcagaaagat catcaatgaa
     6721 acgattcaat tcgcttgtcg tagacaatac tctggaaact tgtgcaagct ccactccatt
     6781 caagtctgct ccgatctcga tccgtttcat atggggacgg atattttcaa gtttaggtat
     6841 agggatcccg cctcgcagac gttgcacttt caatccatcc tcagtttctt gaagccagtt
     6901 tgtgatcgtt tgtttctcac tcactggtac caattctgcc agttcttctt ttccttgtgc
     6961 agtcactaca aattgtctga ccatctgttt gactttttca aattccaatg tttcaaggat
     7021 tcggttattc attttatcac cttatctttc actatgaaaa aagagctgtg ccggacatac
     7081 gctacgcagc cttcttaaat aaactactct gtacaaaaaa cgaaatattt tccagcacta
     7141 ttttccttaa ttcaaaatat tcgttaccaa agctcataca tcttgttggt aagaaatggc
     7201 gttctctcaa cgataaaacg agcgaaacta tgctcgccga attgattttg gaccacatct
     7261 aatggaacaa aggcaagtaa ctgcaaaatc aaaaatatag taacgtaagc catgattagt
     7321 gacaggatgc ctcctgcaat ccagtctacc tgtctcaaga tcggcacata cgtcaatcca
     7381 tggacaaaaa cccccacaaa tcgggtaagc agccagccaa ttagcaaaat aatcagaaaa
     7441 gcagttcctg cataaaaagc ttcatctagg tgaaaagcaa atacctgatc aaaaaatgct
     7501 aactttgatg ttggggtgac agctgggtat ggaatgtata attccaaatg acttgctaat
     7561 ggtttgtaaa aatgctgggc agcaataaat gacaatacat atccaatcgc gaatatgatt
     7621 tgcaaagcaa acccgcgacg tgctccagaa aaaaacgcaa ttagcaataa aaataaaatc
     7681 agcaaactca gcatgctacg ttcctttctc ttctaacttt tttaaatttt tcttattttg
     7741 ataaaaggat cttaattaga agatttttgt ttaatttgtc cttttcgttt ctcgtttaaa
     7801 atctgttgtg cttccacatg atttttaatg gtatgatctg cttgaccatt ttctttcaat
     7861 acttcccgag cttcgttctc gattgcttca atgcgtttca cacgattttc aagctctgta
     7921 aatttgatca tttttttctt taattctgct gtttcttctt ccagctccaa gattctttct
     7981 tgtttcttca attgatccga aagcgcattg acagccatca agattgctgc ttgttcatta
     8041 tccatttgtg gtgataattg tttcaattct gctaactgtt cattgatcag cttcgttacg
     8101 atatccatat gatgcttggt ttcccgtccg ataatagtat atgtttggtt ggcaatcact
     8161 gctttgtagc gtgttttttc gtgcgccata cgaatcattt cctcctattt gatgaaagtt
     8221 ttatttagtg taccatgaag ttggttcttt caccgttctc cacaaatatt tttcacatac
     8281 ttaactatta tatgataaac tttaaaaaaa tgctatgtct cgcaatctct tagaaaacga
     8341 aaagtcgtga gttgcggtat agttttatgt ataaaaacga ggagaatttt atgagcaacg
     8401 tcattatcaa agcaacaaac gaccaactta gtcaaatgaa agcatactat cgctcttatt
     8461 tgttaaataa acagattcct tatactagct ttgctgcaaa aaaagacggg acgaccatta
     8521 ccgcttatac atccggaaaa gtgatgtttc aaggtacgaa tgcagcaata gaagctgata
     8581 aatgggggga ggcacaggat aaacaagcca agccagcaaa aacagcctct tctttgccaa
     8641 aaaatatcag tcaattatcc gttttaggta gtgatgaggt cggaaatggc agctattttg
     8701 gtccgctaac agtatgtgct gcttatgtga aacgtgatca gcttgcagaa ttacgtaaac
     8761 ttggtgttag agattcaaaa gagttaaaag atagccaaat catccagctt gctgacatct
     8821 taaaaaaaac catcccctat aaactgcttg tcttatctcc agaaaaatac aatgagatcc
     8881 aaccaaaata caatgccgtt cggatgaaag tcgctttgca caatcaagcg atccatttgc
     8941 tgctaaaaaa aatcgagcca gaaaaaccag aagcgatttt gatcgatcag tttacgccgg
     9001 aagcaaattt taaaaaatat gcgcgtttag aaaaaaatca attacaagaa aaactgtact
     9061 tcgttactaa aggagaacaa tatcatttag ctgttgctgc tgcttctatc atcagtcgtg
     9121 ccagcttttt ggaagagtta cacaaagcat cagctgaagc tggaatcacc cttccatcag
     9181 gagctggaac gaaatctgac caagtagcgg caaaactatt ggagaaaggc ggcatgccta
     9241 tgcttgaaaa atatgccaaa cttcattttg ccaatacaga aaaagctttg aagctgctaa
     9301 aaaaataatg gaactgtgac aaaagtcatg tcacagtccc ttgttccgaa taaacggtgt
     9361 tctcatgcca actcctcggt cacaagtcac aatagtcatc aaacatgaga agctgttttt
     9421 tgaaattgct ccttgtgacg cggagttaaa cggcaggctc acaacctctt ccttaaacgg
     9481 tgttcaaaag cttgcatctg cgataataag cccggccaaa cgaaaaatat gaggagctat
     9541 ttttgtttgg ctgggcttat ttattactga aaataagtgg tcaatgcttg tgtcaaactt
     9601 gcagcaatct gctgctgata actgtctgtt actaattcct ttaaatcact atcattattc
     9661 atataaccca tctcgattaa aatagaaggt tgaagatttt ctcgaagaac ttgatagttc
     9721 ccagtggaga ttcctcttga atccagaggc agatcagtga aggagtcagc aattgtctgt
     9781 gctaatgttt cctcttgtcc atagtaataa tatgtcgtga ttccagttgc ttcattcgca
     9841 tattccgtag agtctgcgtg taaactgata aagacatctg cctttttttc ttcactgtag
     9901 tcgcatatat ctccaagctg tactaattca tctgtagtat gggtcaaata aaccgttgct
     9961 ccagcatcct ctagcgcctt ttttactttt atggctgtac ttaatgttat atctttttct
    10021 tctgtttgtt catcattgct taatgcccca gtatcatttc cgccatgccc aggatctaat
    10081 acaatcactg catcagatag ctcttccggt tttgtttgta tattctttgt gactttggtt
    10141 ccatcttcga gggctaaata tccagtatag ccatcatttg tcttgatctg ataagcattt
    10201 tttgtttcag aaagaaactc cgccagctct ccgccgttta tagttgcaag tggggtagtt
    10261 agcgagtcat ctgtatatag tgtacggtca ttttttccga ttttgattaa tgtctcttgt
    10321 tctgtatcat caaatgattc ctttgtcttt gtaaccgtac tgttcgtact gctgaccgtt
    10381 tgtccaacta tctttttttg accaaaaaca tctatttgaa aacagataat aatgataatg
    10441 attgcaaata caaaagctcc aattattttt ttcattcttg actctcacct cgtagatcca
    10501 gactaagggt caaaacttaa aatcagctta agtacgcaaa aaaggggctg taaaagatat
    10561 aaccgtagaa aataacatta ctgctaaaat gatttttttc atttttggca tatcctgaaa
    10621 tctccttttg actattcgtg gtgattcatc tacccaataa atagtttcct tcattctaca
    10681 aacaattaat ttcttcgtta ttaaattaaa acacttcctg gaacaccgac ataataaata
    10741 aaaatatacc ctgataatca tgagagaata acaaatttgt atttatgaaa aactacctat
    10801 ataaaaaaaa caagatataa cgatcttcgt catatcttgc ttcttcacct aactattttt
    10861 ctccctatat aacagcttta cgcttctcct aatacaccat tcaaatataa atcgatcaat
    10921 tgcgccacat ttagcggttc ttccacaggt cttttcgacc gagtgatgta aggtgctaac
    10981 actaagaaaa aatgccttgc tgcgatttct tttgaaatcc catttcttag cacagattgg
    11041 tttgctgaaa agatttcttc aaaaggttca cgataatttt tcttccatag aaagaaaaga
    11101 tgatctcttg tttcctttga aagattgctt tcaagatcgt gaagcatcag aaacaaatcg
    11161 accagatgtg tctcaatcaa aaactgcgcc atttttgtca aggttacttg aaatcccgtt
    11221 tcgcttgttt gaacgatttc tctcaaagct tttccaacat cttttaagtg gttttctaaa
    11281 actgccgtat atagtttttc tttatcaccg aaataatgat agagattggg ttgggtgatc
    11341 ccaacttttt gtgcgatctc tcttgttgaa gtgccgagat atcccttgga cataaacaag
    11401 tcagtcgctt ctttcaaaat tttttgctgt gtatctttgt tgtatcgatg gaccattttt
    11461 ccacctgctt tttggttatt ctttttcttt caatacacca tcttccatca taaatattct
    11521 atcgcatttg tcaatcagcc gtgtatcgtg agtaaccatg atcgttgctt tatttttgtc
    11581 ttttgattct tttgccaaga tatcgactac ttctcgtgcc ttttctgaat ccaaactagc
    11641 agttggttca tctgctagaa taatggacgg atcattgtac aatgcacgag cgattgcgac
    11701 cctttgccgt tctccaccag atagttcttc tggatacttg ttgatcaact tatccacgcc
    11761 aagctgctca aataattctt ttgcttcttt ttgacgatgt tcatgtttga tcttatctac
    11821 taataataac tgctttttta ctgtcagaaa aggaaccaga ttggaagctt gcaaaataaa
    11881 gccaatttct ttaaaacgta gtttaacgcg ttcactttct ttttgattac tgaattcttg
    11941 atcattgata aatacagagc cactgctagg cgtctgcaat ccgcctgcca aagtcaaaaa
    12001 agtactttta cctgaaccag aaggaccgat gattgcaaca aattcccctt tatcgatact
    12061 catggttgtt tctttcaatg ctttaactac ttggtcgcca tcttggaaac ttttttctac
    12121 ttctttaaat tctattgctt tcatgctctt cctccttctt atccaattgc cttcaacgga
    12181 tcgattttta cgattgtccg aacagaaaat agtgcgccta acacagctac taccagcatc
    12241 aacaagctaa tgacgccaaa aaacagccaa ttgctttgga atggaaccgc atcaggtaga
    12301 acaagggatg ttcccactgt tcctaataga ccaagaagaa taccaacaaa tgataaaatg
    12361 aatgtttgtg ccactacaga acgcgcaata aatcctcctg tgatcccttg tgctttcatt
    12421 acaccaaaga tattaatctt ttgcatcgtc agtacataaa tgaaaatccc gatcacaata
    12481 gccgcaataa cgatcaaaaa tccgatcata aatccaaaag tcaatacttg cgcattatat
    12541 ccaggtaatt cattgatgaa tttagaaatt ttgatctgtt ctaaatcttc aggtaaatta
    12601 ctgattttac ctctgacaac tattgcgtta atccgtgcat tttcacttgt atcttctttt
    12661 tcgaagcgga tctcttggta agcattgatg gttgtataga gaactggtga gacattgaat
    12721 ttggcatgat ccgtaaagcc agttagttta aacgtcttgt cactaccaga taacttaact
    12781 gtatctccta cggctagacc atattcttct ttcaaactaa tatcgcctac tgcctcatca
    12841 tcgttatcaa aagcttttcc ttcaacaaga ttgggcatga tgaattgatt tttatcgata
    12901 ccgaagaagc taacattgat ttttccttct tctgtactgt ctttactagt cacgactcca
    12961 ggtgtctggg caagatacgc cactttccca ccttcgactt cttctgctat cttcttagta
    13021 atcatggaca tccctagatt tgaatttgca tctttcgaaa gcacaatcga atctgcctcc
    13081 cacttgtcga ctgctgttcg attatcttga gctaagccat aagctaaccc agtcaaaaaa
    13141 aataccaggt aggcgatcaa aaacatcacc cctgcaacta acgcataacg taactttgaa
    13201 tgcataattt catttaatgc taaaaacatt taactcctcc tcctgaaata atcattttat
    13261 acaacaattt ta
//
LOCUS       DAHUWM010000073        13212 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHUWM010000073 DAHUWM010000000
VERSION     DAHUWM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.073"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            147..1016
                     /locus_tag="NK788_001424"
     CDS             147..1016
                     /locus_tag="NK788_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCH6769761.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="NK788_001425"
     CDS             1225..2250
                     /locus_tag="NK788_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCH6769762.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="NK788_001426"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="NK788_001426"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCH6769763.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="NK788_001427"
     CDS             3333..5036
                     /locus_tag="NK788_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6769764.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="NK788_001428"
     CDS             5038..7071
                     /locus_tag="NK788_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCH6769765.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="NK788_001429"
     CDS             7082..7522
                     /locus_tag="NK788_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6769766.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="NK788_001430"
     CDS             complement(7722..8849)
                     /locus_tag="NK788_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769767.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="NK788_001431"
     CDS             complement(8852..9529)
                     /locus_tag="NK788_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769768.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="NK788_001432"
     CDS             9793..11559
                     /locus_tag="NK788_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCH6769769.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="NK788_001433"
     CDS             11574..12011
                     /locus_tag="NK788_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6769770.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="NK788_001434"
     CDS             12024..13175
                     /locus_tag="NK788_001434"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCH6769771.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAHUWM010000074        13193 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHUWM010000074 DAHUWM010000000
VERSION     DAHUWM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.074"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            99..746
                     /locus_tag="NK788_001435"
     CDS             99..746
                     /locus_tag="NK788_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCH6769772.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="NK788_001436"
     CDS             1139..3784
                     /locus_tag="NK788_001436"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCH6769773.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="NK788_001437"
     CDS             4094..5407
                     /locus_tag="NK788_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCH6769774.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="NK788_001438"
     CDS             5397..6050
                     /locus_tag="NK788_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6769775.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="NK788_001439"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="NK788_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="NK788_001440"
     CDS             6787..7872
                     /locus_tag="NK788_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6769776.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="NK788_001441"
     CDS             7869..9761
                     /locus_tag="NK788_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6769777.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="NK788_001442"
     CDS             9768..10145
                     /locus_tag="NK788_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6769778.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="NK788_001443"
     CDS             10299..11081
                     /locus_tag="NK788_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCH6769779.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="NK788_001444"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="NK788_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCH6769780.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="NK788_001445"
     CDS             complement(11996..12055)
                     /locus_tag="NK788_001445"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCH6769781.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="NK788_001446"
     CDS             12447..13109
                     /locus_tag="NK788_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCH6769782.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         4539 a         2021 c         2715 g         3918 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaa
//
LOCUS       DAHUWM010000075        13113 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHUWM010000075 DAHUWM010000000
VERSION     DAHUWM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.075"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            488..814
                     /locus_tag="NK788_001447"
     CDS             488..814
                     /locus_tag="NK788_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769783.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(931..1275)
                     /locus_tag="NK788_001448"
     CDS             complement(931..1275)
                     /locus_tag="NK788_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769784.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1351..2598)
                     /locus_tag="NK788_001449"
     CDS             complement(1351..2598)
                     /locus_tag="NK788_001449"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCH6769785.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2595..3401)
                     /gene="proB"
                     /locus_tag="NK788_001450"
     CDS             complement(2595..3401)
                     /gene="proB"
                     /locus_tag="NK788_001450"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCH6769786.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            3631..4158
                     /locus_tag="NK788_001451"
     CDS             3631..4158
                     /locus_tag="NK788_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCH6769787.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            4221..5591
                     /gene="radA"
                     /locus_tag="NK788_001452"
     CDS             4221..5591
                     /gene="radA"
                     /locus_tag="NK788_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCH6769788.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            5625..6782
                     /locus_tag="NK788_001453"
     CDS             5625..6782
                     /locus_tag="NK788_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCH6769789.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            6952..8412
                     /locus_tag="NK788_001454"
     CDS             6952..8412
                     /locus_tag="NK788_001454"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCH6769790.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    8409..8648
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8734..9270
                     /gene="cysE"
                     /locus_tag="NK788_001455"
     CDS             8734..9270
                     /gene="cysE"
                     /locus_tag="NK788_001455"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCH6769791.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            9267..10679
                     /locus_tag="NK788_001456"
     CDS             9267..10679
                     /locus_tag="NK788_001456"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCH6769792.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            10676..11071
                     /locus_tag="NK788_001457"
     CDS             10676..11071
                     /locus_tag="NK788_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCH6769793.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            11064..11924
                     /gene="rlmB"
                     /locus_tag="NK788_001458"
     CDS             11064..11924
                     /gene="rlmB"
                     /locus_tag="NK788_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCH6769794.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            11928..12458
                     /locus_tag="NK788_001459"
     CDS             11928..12458
                     /locus_tag="NK788_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCH6769795.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            12535..13113
                     /locus_tag="NK788_001460"
     CDS             12535..13113
                     /locus_tag="NK788_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6769796.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT         4313 a         2432 c         2861 g         3507 t
ORIGIN      
        1 caggaggttg taaaatcagc gttttgtcct gagtattaga gaaacaaacg gaaaaatagt
       61 tcctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga acaccgttta
      121 acagaggttg taaaatcagc gttttatcct aagtattaga gaaacaaaac ggaaaaatag
      181 cttctcatat tttaccgttt tttgagctaa taccgagggt cagctgattg aacgccgttt
      241 accaagaggt tgtgagactg tcgtttaact ctgaatcaca aggaacaatt tcaaaaaaca
      301 gtttctcatg tttgttgaat attgtgactt atgattgagg agttggcaga agaacaccat
      361 ttattcggaa aaaggggatg tgacacgatt tttgtcacat tccctttttt tctcaaaata
      421 atgaaactgg aaaattttca gtaggtaagc taaaattcat ttaagaaaaa gaatgggggg
      481 ataatagatg ctggaagaat attttcagga agtcgggttg acactcgttg aattacccat
      541 tgaattttca gatgatcggt tgatttatcg tggctatatc aaagaattgc caataatcaa
      601 tgcggaaggt gaaactagaa aagcgatgca gcaaaaaata gctggtatgt atcaggtgta
      661 tcgggaacag ctactggctg aatcagcgga agaagaaaaa gaaaaaacga tgaacttgtc
      721 catggatgag ctgcttcgtt attatgatgg cgaaacattt gacggatttt cgcttcttaa
      781 cgaagaggaa acagatttac caggcagctg gtaggcgaac gccgtttata tggaaaaaga
      841 ggctgtgaca aaatctttgt cacagcctct aaaaagtccg gcagtttttt gccgatcttc
      901 aaggcactct acttatcgta cggatatttt ttagcggaaa gtccgattca agtggttttt
      961 attcagctga tatttataag ataagtgaaa aatcccagca atcagtagcg taatcgtata
     1021 agcgattgaa tgatcgaaaa aagaatagta attgattgtc gctaataagc ataatgcaac
     1081 catgtaacta atgtgcaagc gtgtaaccag ccctttttgc ccgattttgt caaaatactg
     1141 cgaccatttt tcccgttgac tccctatgct tcgttttttg aattgaaaga aaagggataa
     1201 aataccgact cccagaaaag cagcgatatt cagagaaata aggtaaataa aaaaaatcat
     1261 taaaatttca aacatattga cacctttcta gttggataga acctttacag aaaaaaggca
     1321 aaaggcggga ataatgtttc acacgaaaca ttattcgcgg atttgtccat cgccataaat
     1381 gatatatttt gtggaagtta attcatttaa ccccattgga ccacgggcat gcaacttttg
     1441 agtagagata ccaatctctg caccaaaacc aaataccgat ccgtctgtaa aacgagtgga
     1501 ggcattgaca tagacagccg ccgcgtccac ttcatttaag aaacgttgac tagccgcata
     1561 actatctgtc acgatcgttt ctgaatgttt agtattatgc cgattgatat gtgcaattgc
     1621 ttcatccaaa gaatcgacca ctttgacagc aaggatataa tctagatatt cagtatccca
     1681 atctgtgtct tctgctaaag cagccgtctc tagatattct aacgcacgag catccgcacg
     1741 taaagagacc ccgtgttcta ccagttcttt ttcaatagct ggcaagaaaa aaggtgcgat
     1801 ctcagaatgg atcagcaatg tttctgccgc gttacagaca gatggacggg aagcttttgc
     1861 attgacgatg atgttcactg ccatagataa ctgtgcttct ttatcgatat atacgtgatt
     1921 atttcctgta cctgtttcaa tgataggtac cgttgcctgt tctttgactc gttggattaa
     1981 ttttgctccg cctcgaggaa tcagcacatc taagtattct gtcaatcgca tcaactgtcc
     2041 tgcagtttca tgagaagtat catcgacaaa ctggatggca taaggagatt cgccagcact
     2101 gaccagtact tgctgcaata attggactaa taattgattc gagaagaatg cttctttgcc
     2161 accgcgaagg ataacggcat tccctgattt gaatgctaga ctagctgcat ctgtcgtgac
     2221 atttggacga gattcgtaaa tgatgccgat cacgcctagt ggcacgcgct ttttgccgat
     2281 tctcaatccg tcggtgttcg tccacatctc atctactttt ccaataggat cgtctaacga
     2341 agcaattttt tcgatctctg tagccatggc attgattcgc tcaggtgtca gctgcaagcg
     2401 gtcttgcatc gtttcactga tattgttttc gactgctttt tctaagtctt tatgatttgc
     2461 ttcaatgatc gcttgttgat tctttttgat ggtttgtgcc atttgaacaa gtagctcatt
     2521 tttgcggttt gtaggcataa gtgctagttg ttgcgcgcta tcttttgcct gttgtcctaa
     2581 ttgcatcaag tcagtcattt caagtcctcc ttaaataaag tacctacttc ttttcctgca
     2641 agaatatcaa aaataattgc cggatttttt ccatttgcta aaatcattgc gctgtttgct
     2701 tcaaatatcc tagaagcggc ttttaattta ctctgcatac cgcctgttcc aaatgtgcta
     2761 ccagcaccac ctgcttgtgc cagaatcttt tcatcgattt tatcgatata gcgatacatc
     2821 tgagcctcgg gatttgtagt cggattatct gaataaaacc cgtcgatatc agaaaggacg
     2881 atcaaaaggt ctgccgcgac aagttttgcc atgatggctg acagctgatc attatcgcca
     2941 aattttgtca gatgatctaa ttcatccact gctacagtat cgttttcgtt cacgataggg
     3001 atcgtgtcca taatcaaaag ctgttccaat gtattcctca catttttacg actttcagga
     3061 taatctacca cgtctcttgt cacgaggatc tgtgccgtct gttgattata tacactgaat
     3121 ctttgattat agatattcat cagttcggct tgaccaacag ccgccgcagc ttgctgttca
     3181 ggaatcaaag tcggacgttc tgacaaatgt aatttatgca taccaacgcc tacagctcct
     3241 gatgagacta agatcacttc ctttccccga tttctaagat cagaaaggac aaaagctaac
     3301 tgatcgattg ccgcaagatt gatatttcca ttgggataaa tcaatgtact tgttcctact
     3361 ttgacaataa ttcgttttgc atttttaatt tctttacgca tgtgacacct caatcaaata
     3421 tccactcatt aatttcgttc tattctactt gttttgacga gtaaagtcat gtaaaaaata
     3481 gtttttttgt acaacttgca gtcgtttgga aaaagaacga attaatggtt tactttaaga
     3541 tagcagaatt tcttgaagaa tttgtaaaga aagtctttta cagcctcgga tatgttagaa
     3601 taggagtagt ttatttaggg aggaaaaccg gtgagaaaaa gaggatttga agttatttct
     3661 aaatataaag atcaaaatat caatctaccg cagcgcgcta cacatcatgc tgctggctat
     3721 gattttgaag cagcagaaga ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag
     3781 gggttggaaa cagtcgcaga tggattgaaa aaagatgtat cagaaaatga aaaacaattg
     3841 aaaccaaccc tcgtgcctac tggaatcaaa gcttacatgg gagaagatga atttttgcag
     3901 cttgctaacc gttcaagcaa tccgttgaag cggtttttag tgttgacaaa cggagtaggt
     3961 gtgattgaca gtgattatta caataatgag gcaaatgaag gccatatcat gttccaattc
     4021 accaactttg gtttcaagga tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc
     4081 tttttgccgt ttttaaaagc agatcaagat caaacgactg ctcaacgtca aggcggattt
     4141 ggttcttcca gtaaataaaa cgttaaagat acttaagctt gatttaatgt ctcatgatta
     4201 ggataaaagg aggcggtaca atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt
     4261 gcgggtatgt gtcgcctaag ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga
     4321 tggtcgaaga aatcgaacaa gatacaacgg accgcagaac tcggacaagt ttgacaggag
     4381 aaaaagcaaa accaacaaaa atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa
     4441 caaaattaga agaactgaac cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct
     4501 tgattggtgg cgatccaggt atcgggaaat ccactttgct gttacaagtt tctcagcaat
     4561 tagcagccat aggcggaaaa gtcttatatg tatccggcga agaaagcgca gagcagataa
     4621 aattacgcgc ggaacgcctc ggaagtatca acacagaatt ttatctatac gctgaaacag
     4681 atatgaacga aatcagtcgt gccatcgaac atatctcccc tgattatgtt atcattgatt
     4741 ccatccaaac gatgacgcag ccagacatca caagtgtggc aggaagtgtc agccaagtaa
     4801 gagaaaccac tgcagaacta ctcaaaaccg ctaaaacaaa tgggatagcc atttttatcg
     4861 tcggtcatgt taccaaagaa ggctcgatcg ccggcccaag aatgttagaa catatggtgg
     4921 atacggtgtt atattttgaa ggtgaacaac atcatagttt tcggattttg cgagcagtaa
     4981 aaaaccgatt tggctcaacg aatgagattg ggatttttga aatgcatgag catggattgg
     5041 aagaagtcgc taatccttca cagattttct tagaagaacg cctagacggt gcaactggct
     5101 ctgctatcgt tgtggcaatg gaagggacac gtccaatttt ggtagagatc caagcattag
     5161 tcacacctac tatgttcgga aatgccaaac gaacaacaac aggtcttgat ttcaatcggg
     5221 tatctctgat catggcagtg ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg
     5281 cttacttaaa agctgcaggt ggggtaaaat taaatgaacc agcgattgat ttagctatcg
     5341 ctgtcagtat tgcttcaagc tataaagaaa aaggaacaca gccaacggaa tgtttcatcg
     5401 gtgaaattgg actgactgga gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag
     5461 aagtccaaaa actcggcttc acaaaagtat acctacctaa aaacaactta ggtaactggg
     5521 aagcgccaaa aggaatcgag atagttggag tagcaacttt ggctgaaacg ttgaagcgtg
     5581 tgttccgcta actaataaat gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca
     5641 ttactttgct gatgatcatt gtcggcgcaa gcttgggtat ttcattttta ccctcagctt
     5701 ggcaggcaat cggtcaacag tcaaacggat ggctgaataa tacatttacc aacagcctgt
     5761 taggcgcact tattttcttt ttattatctt tgctgttggc caaacatatc tcagctatta
     5821 tcaaacaagt agaacaaaaa ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga
     5881 tcggtgcgat catcggattg actttaggtg ttgtgatctc gacccctctt tacaatatgg
     5941 aaattccatt cgtcaatagt gtattgccga ttttactgat gatcatctta ggctatctgg
     6001 gattgagaat gggaacgaca cgaatcgaag aatggaagaa agtttttggt tcacgaagca
     6061 aaaaaatcga gcaagaaaca gctagtcaag ttgagagtca actgttagaa cgaaaatcag
     6121 gagaaaattt ccataaatat aaaatcttgg atacaagcgt gatcattgac ggtcggattt
     6181 atgatattac aaagactgga ttcttagaag gaaccttgat gatccctaat tttgttcttt
     6241 acgaactaca atacatcgcg gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg
     6301 gattagatat cctaaatgcc ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg
     6361 gagactttga agatatcagt gaagtagaca gcaaattgat caaattggct aagcttttag
     6421 atggtgtcat tgtaaccaat gactataact tgaataaagt ctctgaattc caaaatgtac
     6481 ctgttcttaa tatcaatgcg ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa
     6541 cgatgaatgt tctagtagta aaagccggga cagaacgcca acaaggagta gcctaccttg
     6601 atgacggcac gatggttgtc gtagaagatg gacagcatta catgaatgaa cgtatcgaag
     6661 tcgttgtaac aagtgccctt caaacagctg ctggacgcat gatctttgct aaacctgcac
     6721 atgcaggacg aggaatcgac aataaaaaag agacagagaa aaagactaaa caatcaaaat
     6781 aaaaaagaaa ttcagccaac tggtaaggaa attcaaccag ttggctttac tatttacctt
     6841 agaaaaggta aaatttgtgc tgagtggctt taaataaatg atatttgaga agccgtaact
     6901 gacggaattt ttgctgttag tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa
     6961 gtacgcgtac gctatgcacc aagtccaact gggcacctgc atattggaaa tgcaagaact
     7021 gcattattca attatttgtt tgcccgccac aatgatggag actttattat tcgtattgag
     7081 gatactgacc aaaaacgtaa tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct
     7141 tggttgggaa tgaattggga tgaatctcct gaaaatcctg gagaatacgg cccttaccgt
     7201 caatctgaac gtaaagaaat ctatcaaccg ctgattgatc agctgcttgc tagcaatcga
     7261 gcatacaaat gctattgcac agaagaagaa ttagaagcag aacgggaagc acaacgcgca
     7321 agaggagaaa tgcctcatta cgcaggaaca tgtgcgaact tgacacctga agaacaagca
     7381 gaaaaagaag cacaaggtct agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa
     7441 tacgcattta cagatatggt gaaaggcgct atttcgttcg aatccgataa tatcggtggc
     7501 gactttgtca tccaaaaacg cgatggtatg ccaacttata attttgctgt tgcggtcgat
     7561 gatcatttga tgaaaatcac tcacgtttta cgtggagatg accatattgc caatacacca
     7621 aaacaactga tgatctatga agcatttggc tggacaccac caaccttcgg acatatgaca
     7681 ttgatcatca acagtgaaac aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa
     7741 tttatcgaac aatatcgcga attaggctat ctaccagatg caatgttcaa ctttattgca
     7801 ttattgggct ggtcacctgt gggcgaagac gaaatcttcg aacaccaaga attgatcaaa
     7861 atgttcgatc caaaacgcct aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa
     7921 tgggtaaaca accattacat caaagcaatg gatctagatg catttactga catgtgtctg
     7981 ccttatctaa tcgctgatgg acgtgtagca gaaaacccag atgaaaagac aatcgaatgg
     8041 gtgaagaaga tcgttagcct gtatcaacct caaatgagct atgctgcaga aatcgttgat
     8101 ctgtctacgc tatttttcaa tgaacatcct gtattgaatg aagcagcaaa agaagtactt
     8161 gctggtgaaa cagttcctac tgttctccac gcattcaaag cacagctaga acaaatggaa
     8221 gtagtagatg ctccaactgt caaagctgcc atcaaagctg ttcaaaaaga aactggcgta
     8281 aaaggaaaaa atcttttcat gccaatccgt gtagctgtat caggacaaat gcatggtcca
     8341 gaattgcctg aaacgatcga attgcttggt cgtgaaaaat cattagcaca tttaaatcaa
     8401 gtactgaact aaacacgatg aacagaaaaa agtaagcagc tgatcattac aagagagata
     8461 ccgggtggtg caaggtatca atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg
     8521 aaacacagaa aacaggaaaa actgttttat aggaaacaaa gtagccaaga acgtataaca
     8581 ggcgttagct gtcagagata atcattgatt atgaagaaaa gtggaaccac gcggaaagcg
     8641 tcttttgata aggaaactta tttaaaaggc gcttcttttc tttataagaa gatgatttcc
     8701 tacataaaat tcttacgaaa ggagtggaaa gtcatgagtt ggtttacccg agcgattgat
     8761 gcagcaaaga gaaacgatcc ggctgcacgt tcgacagctg agatcgtttt gacataccct
     8821 ggttttcatg ccttgttttg gcatcgtttc tctcattttt tatatcagca ccggctcttt
     8881 ttattggcga agatcaatgc tcaattctgg cgtttcctaa ctggtgttga gatccatcca
     8941 ggagcaacga ttggaactgg cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa
     9001 accgcagaaa tcgaggacga tgtgatttta ttccatggcg taacattagg cggcacagga
     9061 aaagaaacgg gtaaaagaca cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg
     9121 cagatattag gaccggtaac gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg
     9181 ttgaaagatg ttccagacga cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc
     9241 aaaggagaaa aagtgaggag atcagaatga taaaaatcta caatacattg accagaaaaa
     9301 aagaagaatt catccctata caaaagggac atgtcggtat gtatgtctgc ggaccgactg
     9361 tgtataatta tatccatatc ggaaatgctc gtagcaccat tgctttcgat acaattcgac
     9421 gctattttga atatcgaggc tataaagtcg atttcgtatc gaatttcacg gatgtagacg
     9481 ataaaatcat ccgtacagca aacgaattag gtattacagc accagaagtt gctgatcgtt
     9541 tcatccgagc atttgaagaa gatacgaaag cgttgaatgt cgaacctgct acactgcatc
     9601 caagagtcat ggatcatatg ccagatattt tagcatttat tcaagtgttg gttgaaaaag
     9661 gctatgctta tgaatcttca ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg
     9721 ggaaattaag tgaccaatcc atcgatgaac tggaagtcgg agcaagtcag cgtacagggg
     9781 aagaacagca gcaaaaagaa gacccattag atttcgcttt atggaaacaa gcaaaacctg
     9841 gcgagatttc ttgggattca ccatggggca aaggacgtcc cggttggcat attgaatgtt
     9901 ccgttatggc aacaaaacat ttaggagaaa cgatcgatat ccatggtggc gggcaagatt
     9961 tagaatttcc tcatcatgaa aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat
    10021 tcgctaatta ctggatgcac aatggctatg taacgattgg cgaagacgat gaaaaaatga
    10081 gtaaatctct agggaatttc atcactgtac atgaaatgat caaaaacgtt gatccacaag
    10141 tcttgcgttt cttcatggcg accacacaat accgtcgtcc gatccgctac agtgaaacga
    10201 cgatgaaaga agcagccacc aatttacaac gactgaagaa tacgtttgac aatttgaaat
    10261 tccgccaaga aactgcctca gagaatttgg cagatgatgc ggataaaatc gctttgttag
    10321 aaaatttaga acagcgattc atcgaagaaa tggacgatga ttttaatgca gcaaatggga
    10381 tcacagtggt ctatgagctt gctaagtggc tgaacactta tctagaccag ccgaaagttt
    10441 ctgccgctgt cttggcacaa ggagaagctt tattctcgca atggctttcg atcttcggta
    10501 ttttatttac agcagaagag ttattagatg aagagattga acaattgatc gaagaacgaa
    10561 atcaagcaag aagcaatcgt gattttgtcc gaagcgacga aattcgtgat ttgttgaaag
    10621 aaaaagggat cttattagaa gatacaccgc aaggaacccg atggagaaga gaagcatgag
    10681 agattacaca caattgaacg gtttagcctt ggcttatgtt ggagatgcga tttacgaaat
    10741 ctatgttcgt gaccacctga ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc
    10801 ggcaacgaat tatgtttctg cgaatgcaca agcctttttg attcagcaaa tgctggaaga
    10861 acatcttttg acagaagaag aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca
    10921 tacgagcgca aaaaatgctg atgttacgac gtatcgggtc gcaacagggt tcgaagcgct
    10981 catgggctat ctgcatttga caaaacaaac cgagcggatg gaagaattga tccgctggtg
    11041 tattcagaaa gttggtgaaa aaaatgggta aagaaagaac aaacaaacat caatcgaaac
    11101 gtggatttga agacaaaaaa caaaatagat attcaaataa aaagcaaact ggacgaaatc
    11161 agtcagtttc tgatcaaaat gaagaagcat tagaagataa tttcgtcttt gggcaccatg
    11221 ccgcagtaga agcgctacaa caagggcgag ggaataaact ctttcttcaa gaagattcac
    11281 gaggagaaaa agtagaagaa ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat
    11341 gggttcctaa acaaaaatta gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc
    11401 ttgcgattac accttatgaa tatttatccc ttgaccaact gattgaacga acaaaagaga
    11461 aaaaagaaga ccccttcttt ttgattttgg atagcatcga agatccacac aattttgggt
    11521 cgattttacg gacagcagac gcaagtggcg tcgatggtgt gatcatacca aaacaccgag
    11581 cagttggaat cacacctatc gtaacaaaga cttccactgg ggcagtcgaa catgtccctg
    11641 ttgcacgcgt gacgaatttg gtacaagctg tgcaacagct caaagaaaca ggattttggg
    11701 tatttggcac agatatgacc ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg
    11761 ctttagtcat tggtaatgaa ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg
    11821 aattgctgac gatcccgatg accgggcacg tccagagctt aaatgccagt gttgctgctg
    11881 gtttgcttat gtatcaggct ttttcccaac gtcaggggga gtagaccatg aaaaaacagc
    11941 tgttgattgt agacggctac aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa
    12001 agcaggacaa aatggcggat gcaagagaag ctcttttaca tcgtttatca aattatgcga
    12061 aatatgaagg aatggaagtc atcgttgtct tcgatgccca actagttccg ggcatccaac
    12121 aaaattataa gaaataccag ttggatgttg tttttacgaa agaagacgaa acggctgaca
    12181 gttatatcga gagaatcgct ggtcagctga atgaccgttt gacacaagtg acagtggcga
    12241 caagcgattt agcagaacag tggatgatct tttcccaagg agcattacga acttctgctt
    12301 atgaattata taagacgata aaaaaaacgg aaaaaacaat agcccaagaa aataaagata
    12361 tgcattttat tgatttccga cgtaattctc catggaatga agagcagtta aaaaaattgt
    12421 cccaaaaatt ggacgattta atgagaaaaa ataaataaca tatcgttcta atcagttcga
    12481 tgaatcattg atgataagat tcctccataa acgaatttat ggaggaatta ttttatgtct
    12541 gaggagttat tgcgcgctgc acgtaatggt gacgaagaag catttgctgc cctgtatcaa
    12601 aaatattttc cacttttact acgtttgaaa aacacctatt atttaagaaa ttatgtttct
    12661 gaggattggg aacaagaagc aaggatcgct ttatatcagg cattgagaac ttacgaaaaa
    12721 agcaaaggtg ttagctttgg aagttattat aaaagcgtta taagcaatca aatctattct
    12781 ctgttgagga agcagaacgc gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac
    12841 caaaaaatag agacagaagg accagacttt ttagcagaga tcacattgca agaaccttta
    12901 gcaattcagc atcttttatt aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa
    12961 atagaagcta aaatttttca ttattatatg caaggcgttg attttgtaga catggctgaa
    13021 tgtttagcaa tggatcacaa gcaagtaatg agcgcttacg ggagagtaaa actcaaaata
    13081 aaaaaacact tgaaatttta taatgaggaa taa
//
LOCUS       DAHUWM010000076        13027 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHUWM010000076 DAHUWM010000000
VERSION     DAHUWM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.077"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..623
                     /locus_tag="NK788_001461"
     CDS             <1..623
                     /locus_tag="NK788_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCH6769797.1"
                     /translation="TYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIY
                     DKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVH
                     TAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGM
                     ISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            690..1184
                     /locus_tag="NK788_001462"
     CDS             690..1184
                     /locus_tag="NK788_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCH6769798.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1240..1878
                     /locus_tag="NK788_001463"
     CDS             1240..1878
                     /locus_tag="NK788_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769799.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1950..2345
                     /locus_tag="NK788_001464"
     CDS             1950..2345
                     /locus_tag="NK788_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6769800.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2329..4782
                     /locus_tag="NK788_001465"
     CDS             2329..4782
                     /locus_tag="NK788_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6769801.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4779..6806
                     /locus_tag="NK788_001466"
     CDS             4779..6806
                     /locus_tag="NK788_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769802.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6803..7825
                     /locus_tag="NK788_001467"
     CDS             6803..7825
                     /locus_tag="NK788_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCH6769803.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7842..8771
                     /locus_tag="NK788_001468"
     CDS             7842..8771
                     /locus_tag="NK788_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6769804.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9016..9132
                     /locus_tag="NK788_001469"
     CDS             9016..9132
                     /locus_tag="NK788_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCH6769805.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9148..9951
                     /locus_tag="NK788_001470"
     CDS             9148..9951
                     /locus_tag="NK788_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCH6769806.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10186..10422
                     /locus_tag="NK788_001471"
     CDS             10186..10422
                     /locus_tag="NK788_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769807.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10429..12381
                     /locus_tag="NK788_001472"
     CDS             10429..12381
                     /locus_tag="NK788_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCH6769808.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12453..12950)
                     /gene="dfrG"
                     /locus_tag="NK788_001473"
     CDS             complement(12453..12950)
                     /gene="dfrG"
                     /locus_tag="NK788_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCH6769809.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         4481 a         2161 c         2523 g         3862 t
ORIGIN      
        1 ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa
       61 gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag
      121 caagctccat atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac
      181 ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac
      241 aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca
      301 gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag
      361 ttcctagaga cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag
      421 aaattcaacc tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa
      481 tgattagctc tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata
      541 ttattggaaa gagagaagca aaactaataa taggtatttc aagtacagtt acagattata
      601 ttagcggata ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt
      661 ttataaaaag gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt
      721 ataatgaagg tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg
      781 cagaaagaat cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc
      841 cttttgatgt ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc
      901 aagaaatcga aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta
      961 gtagcttgga agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt
     1021 cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt
     1081 ccaatttaca aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta
     1141 attacctagt tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt
     1201 tataattaaa ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat
     1261 cttaaactat taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt
     1321 cgttacaaac agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt
     1381 ccagaatatg ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta
     1441 gctgtctatg ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt
     1501 ggtatcgaat acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct
     1561 cgatttattc cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt
     1621 ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga
     1681 agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa
     1741 acgagattgt atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc
     1801 agatattcaa tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca
     1861 aaaaataaaa ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt
     1921 ttctattttt acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt
     1981 ggtcagtaga aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct
     2041 ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac
     2101 caccactttc tctgattgac ggagcactac tcaaatactt agggattccc attgttctca
     2161 ctgggttcat gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta
     2221 tcattaccta tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa
     2281 aagaaacaat gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg
     2341 attaaatata tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat
     2401 gaattaattc cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat
     2461 aatttctgtc aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca
     2521 accgaatcaa gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta
     2581 aaagagatag cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt
     2641 gacaatcaag tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa
     2701 gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt
     2761 gtgaatcaca aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt
     2821 tctaagatgg aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg
     2881 gagaaagatg attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt
     2941 gatgactacg actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa
     3001 tacgatattc tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt
     3061 gaacaggaag ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa
     3121 ttggatttcc ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt
     3181 gataccagta tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat
     3241 aaaaagaaag aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt
     3301 actaatgtgg tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc
     3361 aaagaatcta tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag
     3421 ctgaaacaac gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta
     3481 cgaccttttg gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat
     3541 atcaatgact atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct
     3601 acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga
     3661 aatatttatc tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca
     3721 ttagcgtccg cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg
     3781 gtctattatt cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt
     3841 ggtgaatgga aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg
     3901 agtgagaacg acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat
     3961 tcagaaagcc ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc
     4021 gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg
     4081 ttgttattgg tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct
     4141 gaccatatcg aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt
     4201 gtcacccaat ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc
     4261 ttacccgatt ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca
     4321 atgctgattg tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt
     4381 aaaattgttg accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta
     4441 tctatgcgat tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa
     4501 aacgcggacg atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt
     4561 cgttccactg acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa
     4621 gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac
     4681 atctatggtc gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc
     4741 tttgatacca gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat
     4801 ttcttcacta tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca
     4861 cgctgggcaa tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg
     4921 aatactccaa gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg
     4981 gctggcttcc atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg
     5041 ccattactaa ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg
     5101 tgaaagaagc ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca
     5161 tgcagacgat tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt
     5221 tcctccttct ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc
     5281 gagaaacgac gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg
     5341 cttcctttat tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg
     5401 tcagtaccgc cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag
     5461 ggaaagacag tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat
     5521 ggctattgct tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa
     5581 gtttattgtc taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg
     5641 aagaaattga agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag
     5701 gaacagtctt ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa
     5761 caggaatcat gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca
     5821 gtttcctatt aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt
     5881 tatttaatgt gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta
     5941 tttccactat gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac
     6001 aaattgtgac ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt
     6061 tacaaagcaa cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga
     6121 tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag
     6181 ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata
     6241 ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc
     6301 aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa
     6361 atcgtctagt caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg
     6421 tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac
     6481 aatctatggc agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa
     6541 aacgaaagaa cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa
     6601 aagaaaaccc atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa
     6661 ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa
     6721 aagagcaaaa acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa
     6781 cggataagcg aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt
     6841 ctctctcttc ttttttctct tattattcct agtcattttc tttgcagatg atacggacag
     6901 tggcgaaaac aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt
     6961 gttggcacac cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt
     7021 ttcttatatc ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca
     7081 aagttccgaa tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca
     7141 aggggttaaa tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga
     7201 ttctgttatc caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg
     7261 gaaaaaatat acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa
     7321 agcagactat cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg
     7381 aaatcagttt tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt
     7441 tgatgatgaa accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta
     7501 tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt
     7561 gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca
     7621 agaaatctct atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa
     7681 tgcaggcaca tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc
     7741 aggcgaccca atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg
     7801 ggctagacgt gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc
     7861 gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc
     7921 cgatgattaa agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg
     7981 gtagtgtcgg atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg
     8041 aaacagaaat cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat
     8101 cctttgcgaa agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg
     8161 aaaaattgac ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta
     8221 aggatattcc tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga
     8281 atgagaacac ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca
     8341 aggaaactat atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg
     8401 ttattatcaa aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac
     8461 agctagaaag tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag
     8521 aaacgttctt ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta
     8581 atcatgtgtt acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct
     8641 ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa
     8701 caaaggcaac ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga
     8761 ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt
     8821 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta
     8881 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
     8941 cgggaacaaa taattagatg tcctttttta ggagggctta gttttttgta cccagtttaa
     9001 gaataccttt atcatgtgat tctaaagtat ccagagaata tctgtatgct ttgtatacct
     9061 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
     9121 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
     9181 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
     9241 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
     9301 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
     9361 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
     9421 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
     9481 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
     9541 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
     9601 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
     9661 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
     9721 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
     9781 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
     9841 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
     9901 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
     9961 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
    10021 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
    10081 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
    10141 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
    10201 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
    10261 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
    10321 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
    10381 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
    10441 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
    10501 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
    10561 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
    10621 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
    10681 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
    10741 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
    10801 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
    10861 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
    10921 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
    10981 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
    11041 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
    11101 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
    11161 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
    11221 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
    11281 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
    11341 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
    11401 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
    11461 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
    11521 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
    11581 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
    11641 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
    11701 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
    11761 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
    11821 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
    11881 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
    11941 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
    12001 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
    12061 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
    12121 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
    12181 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
    12241 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
    12301 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
    12361 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
    12421 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
    12481 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
    12541 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
    12601 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
    12661 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
    12721 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
    12781 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
    12841 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
    12901 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
    12961 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
    13021 tcctgcc
//
LOCUS       DAHUWM010000077        12985 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHUWM010000077 DAHUWM010000000
VERSION     DAHUWM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.078"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(85..747)
                     /locus_tag="NK788_001474"
     CDS             complement(85..747)
                     /locus_tag="NK788_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCH6769810.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(757..1572)
                     /locus_tag="NK788_001475"
     CDS             complement(757..1572)
                     /locus_tag="NK788_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769811.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1833..2276)
                     /locus_tag="NK788_001476"
     CDS             complement(1833..2276)
                     /locus_tag="NK788_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCH6769812.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2410..2748)
                     /locus_tag="NK788_001477"
                     /pseudo
     CDS             complement(2410..2748)
                     /locus_tag="NK788_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2736..3113)
                     /locus_tag="NK788_001478"
     CDS             complement(2736..3113)
                     /locus_tag="NK788_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6769813.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3337..4530
                     /locus_tag="NK788_001479"
     CDS             3337..4530
                     /locus_tag="NK788_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6769814.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4696..5118)
                     /locus_tag="NK788_001480"
     CDS             complement(4696..5118)
                     /locus_tag="NK788_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769815.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5701..6195
                     /locus_tag="NK788_001481"
     CDS             5701..6195
                     /locus_tag="NK788_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769816.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(6357..7529)
                     /locus_tag="NK788_001482"
     CDS             complement(6357..7529)
                     /locus_tag="NK788_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6769817.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7735..8550)
                     /locus_tag="NK788_001483"
     CDS             complement(7735..8550)
                     /locus_tag="NK788_001483"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6769818.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8700..9146)
                     /locus_tag="NK788_001484"
     CDS             complement(8700..9146)
                     /locus_tag="NK788_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCH6769819.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(9143..10147)
                     /locus_tag="NK788_001485"
     CDS             complement(9143..10147)
                     /locus_tag="NK788_001485"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCH6769820.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(10187..10516)
                     /locus_tag="NK788_001486"
     CDS             complement(10187..10516)
                     /locus_tag="NK788_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCH6769821.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10642..12969)
                     /locus_tag="NK788_001487"
     CDS             complement(10642..12969)
                     /locus_tag="NK788_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6769822.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3738 a         2526 c         2353 g         4368 t
ORIGIN      
        1 atatgtttca aaaaatgaaa aagaatgaca gtttttacat aaaaaaaact gtcattctta
       61 attgaaagtt tcaatttata aagtctacag atccatagtt ctcatcaatt tacgattgtt
      121 gaagtaaatc aaaccaaata cgatagaagg acctagtaga atacaccatt ggggctttac
      181 gaaaaagaag agaaacaaac ttgtcaatag taaaaaatct agtaaactca cataccattt
      241 tttcaacgta tagtagaacg ctatccgtag aacttgattt ggtttcgacg ctggatttct
      301 cacctgaaaa taccagcagt tggtgctaaa ggaaactgat aaacaagcaa gtaaaacaaa
      361 caacggaatc aaccattttc cataagaaaa acgtataaaa aagaaacaat caaaaatcat
      421 tatcacacta actatccaag caaatagcca ataaaaaaat ccctttagcc aaaattggcg
      481 aaacatcttt atgaacaaac gtactggtga aaaactattt tcttttgcta attcttctgt
      541 catacctatc atcacgatgc aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa
      601 gacattcctt ccatctatcg acaaaaggaa cgcagctgct atatacaaga tattgacaca
      661 ccaaaaacaa aggctacttg ccaatgattg atacaaccaa taaaagaatt ccatataaac
      721 atttttgtca aaacgctgct ctttcataat cacctcctat tccttcattc ctgatgaagc
      781 gatcccttct acaaaatatt tttgcatcgc taaaaatata aaagcaacag gaagaacaga
      841 aacaactgag gcagccatga ttaatgcgta ctctgcatca aatagcccaa taaacatttt
      901 caaccctaac tggattgttt tattttccgt agaagacaaa taaatgaatg gccccatata
      961 atcgttccat gtattaacga atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag
     1021 aggtaaaatg atcttgcgat agattcccca ctctgataag ccatcgatcc gcgcactctc
     1081 acataaagaa tctgggatac tactgtaata ttgcttcaac aagaacacac caaaagcatt
     1141 gaacgcttgt aatagcacca acgaccacaa cgtatctgtt aaacccactt ttctcatcat
     1201 aatgaactgc gggatcatat aagactgcca agggacagca atcgtgccga tatatgctaa
     1261 aaataaaata tcgcgccctt taaaatttag tttagaaaac ccgtaagcag caaaactaga
     1321 agtaaacaac tgaatcaacg tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt
     1381 cactaaagga atacgagacc aaataaccag ataattctct aaatgccaag ttttagggat
     1441 ccattgaatc ggaatcgtga acacttcatt atttgctttg aatgaagcag aaaccatcca
     1501 taaaaaagga atgatcgtca ataaagctaa tacaatcagc agaatcatac ccaaccattg
     1561 aataaagggc atgttttttg aacgaatttt tgcttttttt ctttcatttc gaacacctcc
     1621 aaagttataa tcttcatttc tttgtaatga ttacaatcca gaacaacggt tattatagct
     1681 gattcatttc cgttcttctt atttttgtaa gcgcttattt aacaagttat gtaaatctct
     1741 ttattttttt gatttaaaaa tgaaaaaata caaaagcaag actgtcatac gtctcgcttt
     1801 tgtattgaaa tttcttcgat tagagatact tattactgtt caaaagcttc tttgaaacgg
     1861 tgaatcaatt cattgactac aggtgataaa atccgattct ttttccagac tagcatgcag
     1921 ttcgtttcaa tcattgggtc aagcggaacg aatgtcactc gctttgaatg atgacttaca
     1981 gcaccttcta tgactaaagc agcacctacc tgattctcaa ttagcgcaaa tacattgaaa
     2041 atcaaattgt acgttcctgt gatcagcaac tgctcctttt ttatgcctaa ccagtcagca
     2101 aacattcgct gtatttctgg acgctttgag atcaaaagag gtacattcgc aaagtcttct
     2161 tttgttatca cgtctttttt tgctaaaaca gaattttcta aaatgagcaa tccccatttt
     2221 tcctttcttg gtaacaaaat cgtttcgtat ttctctgtat ctactggatc tagcaaaatc
     2281 gctaaatcta acaggctttt ttctagtcac tccgtgatat catcacttgt tccgctgtgg
     2341 atatgaaatt gaacgctggg ataatcacgg atcaactttt ctagtaatag tgccaatgta
     2401 ttcgaactat cagcttccac acagccgatc gtaaatcggc cgctgaataa ttgttttttt
     2461 ctgttctcga attcttgagt ggtttgattc gttaaatcta aaatttcttc tgctcggctt
     2521 ttttagaaaa taacctgctt ctgtcaagat cattttgcgg ttttcgcgta caaacagttc
     2581 tgttcctagt tactcttcta actccttcag ttgtcggctt aaggttggtt gtgtaagatg
     2641 tagaacttct gcggcttgag agattgttcc ttcttctgca attgtccaaa aatattttag
     2701 taagcgtaac tccatagcga tgctccttga tttttttact tgatacattc cattttattt
     2761 ctagataaca cacgatcata tttcttgatt tttccttgta aacgttccaa acattcttgc
     2821 atttcagcaa tttttttcgt caaaagttca ctttcttcaa tcaaaagctt acgccgctca
     2881 aatacagttg cttctccttc gctagatagg cgaatatatt caatcaaaga ctcgatcgat
     2941 aatccggcat ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa
     3001 tcacggattc cgccttttgt ccgattaact ggtggaatca acccgattcg ttcataatag
     3061 cgcaatgtat cggccgaaat gcccttttct tcactgactt gtttgatatt catctgatca
     3121 ctctttctct taatttcttg ccactttact tatgtatttt atttattcta caactttaag
     3181 tgtactccaa gtcaatactc atttctatta aaactaagct ggattatcta tatcggttgt
     3241 ttgtgtatta tttcaaataa aaaagtcttg tttttttatt cttttttgga tatactttta
     3301 acaatttagg gaaattttag gaaaaggggt ataaaaatga caacgtatca gcgaaaggta
     3361 ttagtatcga catcggcggg aattgcttta gagaatatgg atattatgtt tttagctttt
     3421 tcattatctt caatgattac ttcttttcat attagtggta cgcaagcggg tttcatttca
     3481 acgatcacta atttggggat gctcattgga ggtatttttt ttggcatcat agctgataaa
     3541 tatggacgtg tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc ttctttattg
     3601 atgtattttg cttcaaacat ctacttggtt tatctctttc gctttatcgc aggtgtagga
     3661 gcgggaggag aatatggggc gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa
     3721 attggacgag cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg
     3781 attttagctg ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta
     3841 ctgccggtgt tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa
     3901 tctgtacaat ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt
     3961 gcttggcaaa catgtggcct tagtatcatg gtaactgtcc aaattgccgg ctattttggg
     4021 ttaatgaatt ggctaccttc aattatgcaa aatcgattag ggctttcggt ttctgggtct
     4081 tcattgtgga tggtcagtac tattttaggc atgtctttgg ggatgatgac ttttggcata
     4141 atcatggata aattggggcc taaactagcc ttttctttgt ttttaatttg ttcatcctta
     4201 tctgtctatc tacttgtgtt agcccatagc cagtggagtt tggtagcagc cgctgtgata
     4261 gtcggctact ttatcaatgg catgtatggt ggatatggcg cagtgattag tagtttgtat
     4321 ccaacagaaa tccgcgcgac ggcgaataat ttcgttatga accttggacg agctgttggt
     4381 ggtttttctt ctatcgtcat cggtttcttg atggatcatt atagtttaac tgccgtaatc
     4441 ttgtttttga gcggtattta cctagtcagt ctgctggtct tgacaactct tactggtgtt
     4501 acagcattaa gggaaagctt gcagcattga tagttgagtc tgtaaatgac aagaaagagg
     4561 tgtggaaaaa gcttgcatct gcggtaataa gaccagccaa atcaaaaaca tgagacactg
     4621 tttccgtttg gctggtcgta ttacttgatg cagggcagct ttttcaactg ctaatttcct
     4681 tctatctggc ggcgactatt ctttgacgtc cgtcatttca aagtacttta ctaataactt
     4741 agcataacga aaaactgtgg atacactcgt atattgagcg tctgcataat cttccaaatt
     4801 tttctcagga tgttgataca tgaattcaat caaatgataa ttatatgatt ttctgtagaa
     4861 atgaagataa ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt
     4921 attctcattc ttcaaaatga ccttatcagg atattgttgg aacaattctt ctaaatctgt
     4981 tagataattc aagatagact tacttgatac cttcaattcg ttttgtagtt cttctattga
     5041 acaccaacgt tctgtttcaa gcagtaaatg aatcatttta attttctttt gagaaacttt
     5101 tgttaaaaaa gtctgcatat tttctcctcc tgtatctact ttactatact acatacaaga
     5161 aaaaaacgga tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat
     5221 ttctcttttt ttcctaagta ttctattgat atgaatcatt taatcgttta ttatataaat
     5281 gtcaataatc taataataaa ataacgtaca tattttatta ttgcatactt aataacagaa
     5341 ctatagcaaa aaatcccgtg tatcaacacg ggattagatt aagtttcctc aatcactatt
     5401 cataacacaa ttattataac aagtaagtaa acaattgaaa aggacattta ttgaaaacgg
     5461 ttgtttttta cttaaggagg aaatttagtg aaaaaattag ttgcgttgtt tttggtcgca
     5521 ggaagtttgt tgagtgggtg tggtgctact acgtctacta ctcaaagcac aagttcagct
     5581 caggcttcca gcagcagtac cacgatctcc gaaagtacgg cagattcatc gaaagaacaa
     5641 attgaaaaga aaaaagaaga agagaaacaa aaagcggaaa aagctgcact agaaaaaaaa
     5701 gtgacagaag cggaatctgc tttgtcacaa gctgaagcaa atcctacaaa agaaaactat
     5761 gcaactgctt cagcagctat ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt
     5821 cttgctgcaa ttgattccac aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa
     5881 gaagaaattg cacgacaaca agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa
     5941 gcagaacagg cgaggcaaga agcaatcgca cagcaacaag cagaacaggc gcgacagcaa
     6001 gcagcagctg aacaggcttc tcagcaggca caagccgcag ctcctgagca aaataatgaa
     6061 caaactgtct atgtcacacc gaccggttca aaataccata cacataaatg tggaaatggt
     6121 acgtataccc ctgccacttt aagcgaagct caagctcgtg gtttaacccc ttgctccaaa
     6181 tgttatggaa actaattcaa aagttatact ataataataa aattcttctg aaacataaaa
     6241 aaatagaagc tggcgttcca gatagtaaac caaaaatagc agtttacacg tccatttcgt
     6301 cagcttctct cttttttcat ttagtctgtt ccctctgctt ttttggacat cttcattcat
     6361 tctttctcaa tcaaatagta cacctctttt tcttttcgca gtttatatct tccttttttc
     6421 aatttcattg aaaactgttt cgaatgttta tgttttacat ggattttaag caggagctcc
     6481 tcatgttgca agcgatagtt cctgcctttt aaatcagaaa atctcactag tccgtattca
     6541 tcagaagctt tcacaaatgg aactttttct ctagtaatat agtgtttgcc gttgagcgtt
     6601 tttacagaaa agaccattcc ttcgattggt cgtccgtttg tgttctttac ataaaaagat
     6661 aaaggatagt ttcgcttagc gatggccaaa gaatctaaat aagcgatgag tagccaaact
     6721 aagccgattg atccaagtag tataccgata aaccaaataa aaaatttcca ttttttccat
     6781 tgatcaattc cttggatcgc tttttttgcc tcttttgctt gataaggaat acgatgcccc
     6841 gtcacaagca gtcgatgtgt attgaccata taaggagtgc aggtaagcaa agtcacgaga
     6901 tctctgcctt tttggatacc caaagaatct acttcgtcag gtaagaccac ttggattttt
     6961 tctacttgat acgccaatgt ctttccattg atctggatga aaaactggtc tccttttttc
     7021 aacttaggca aatctgtaaa taattttgct tctgggagtc cacgatgccc agaaagaacg
     7081 gcatgggtgt ttttcccgcc agtcggataa gaacttcctt ccaataatga cgttcctttt
     7141 tgcaaaaata tttccgttgt ctgatcgaat ataggtaagc tgacattgat ttttgggatt
     7201 tcgatgactc caagcatatg ttccataaaa aaggcattac taggtaaatc ttttaatgct
     7261 tttgcatcaa ctgcttggtt gaaactgcct atccctggtg atacgttttg ctctgccaac
     7321 tgttggtttt tctttgtcat tttttcctgc tgttttttta tttcctctga attcttattc
     7381 gatgcttgct tttgataatg gatgatcatt tgctgggcaa gaaaatcatt cagtgaatct
     7441 tctacaaaag gataagaaaa gatcccgatc ccaacgacta aaatgattgc catgaagatg
     7501 tccagtattc ttattctgct tttattcatc atgtccacct attttctaat atgtatatga
     7561 agaggttgtg aaatcagcgc cttgacctga gtattagcga agcaaacggc aaaatagttc
     7621 ctcctatttt gccgtttgtt gagcgaatac caagagaccg accacttgtc acaacctttc
     7681 tccccatttg aatcactatc gtttatttat cgtatgattt ttgtttccaa tagtttaagc
     7741 ttctgtttct tgatggcgtt ttttgaaata gatcactgct cctccaacta aagcagtacc
     7801 taccaagatg aatacgatga tccccatacc accagttgaa ggcaagattc ctttaggttg
     7861 gttatcgatc gttgtttcat cagaagttgt tggatcatca tctactgtaa attcagtgta
     7921 cgcagcttca ttcttaggta atgcgtagcc ttctggtgct tttgtttctt gaaggcagta
     7981 tgccccttgt ttcagccctt cgattgaaaa tttcccttct gaatcagaaa tcaatttcac
     8041 ttcttcccag tcagaacctg tgacttcatc tgaccaagca tatgggtatt ctcctgaggc
     8101 aaggtttgct atttttttca ttaatttaac agttggatct tttgctctta gaatagaaaa
     8161 ttctgcatcg gctaattttt cttgcgtgtt cacatcttgt ttgatgaagt ggaaatcacc
     8221 gtagttttgg acattttcta cagtaatcgt gttatccgta gttgtatttt cccgattgac
     8281 tacaccgttt ggattcccaa gattggcttc atctgcgcca tggatgatac cggttgtttg
     8341 ttgcaatttt ccgtttttta ctataaaagt agtttctttt tgtggattgg ttaattgata
     8401 accatctggt gcttttgttt caactaaagt ataattgccg tctattaatc cattgacact
     8461 gatggatcct tcagtatcag ttgtgaatgt caacgctgac tcagcaggat catagagagc
     8521 gacaccagtg ttttcatcaa aaccggtcac gaattgattc tctgaatttt tgatctggaa
     8581 ttgcgcccct gctaacttat ctacccctga aaagttccca gctttctgga ttttgatatt
     8641 gctttctttg atgacatttt tagcatatag gtgaatcgtg tctaaaggtt tgtcagggtc
     8701 tagggtaagc ttaacacgat atttggcgac gtttcgacac cgggctttgg tgtttctaca
     8761 aagacgtata cagcatcttt cccatcagat ttacctggaa gagtcaggtt agctaatcct
     8821 tcaccagaag tcgttacact tgcgaagggt gtagcgtctg ctggaactgc tgtgtcttta
     8881 cttggatagt acgtagcagt gatgtcatat acgttgaatt caacgccgtt caacggttcg
     8941 ctattcggaa atgtcaattc cgttccgttg tttatttgat tttctggtgc tgaagtgaat
     9001 ttcaatttat gcaaattaac cgtaacattt tctggtgtag gtgatacggc tgcagaaacc
     9061 tctgcagttc ctaggccgag aacgaaagga agcagcatga tagcagttcc taaaaacgtc
     9121 catatttttg ttttgatgtt tttcatttta tttccctcct attgtttcct ttttgttgtt
     9181 ttttataaaa ccaagctgtc cctgttgaaa ggactagcaa taaccctaaa attgagaaac
     9241 ctccaagcat ctcttcgctt gtttttggaa agcttcctcc atttttttca tttgatggac
     9301 catttgtctg catctgaact ggcgtttttc cattattcgt cggatttgct tttggttcgt
     9361 tgatgatttc caatggtata ggatgaagcg gatcataact gcctttttca acttcaaaga
     9421 gaatggttga ttgactcaat atatatcctt taggcggttt aatctccact agttcgtaac
     9481 ttccaaaaga caatccatgg acagaaaatg ttccatcttt actggaagta aaaatcgttg
     9541 ctcttgaatc atctttggct acccaatgat ttatcccgtt ttcttgcgct aagtattggt
     9601 catttttttt gactagaaat tgagcatctg ctagtttttg tttcgtttgc cgatccactt
     9661 tgacaaattg tttgcctcct gtttggatgg caagatgtgt ttctactgtt tgattgtcag
     9721 tcgtcaaacg tacatcgtta tcaaatgtcg ttttttcact gttttctttc aatgtcattt
     9781 ggtaacggat cgttaatttt tttccagcaa acgaactaag atcttttcca gaatctgata
     9841 agacaaaacc atgttttatt ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca
     9901 aaaaatcgat cgtttctggt tctagccata actgaggatc agctgtatct ttgatcgtat
     9961 aggttcctat ctcgttgatg ttctcaggaa ttgttacggt gatgagataa gggactttat
    10021 ccccatctgc aaaatctgtc tgcttattct ctactgtttt tgttaatgtg agtgcagcag
    10081 gtattttttg ttcgctcttt ggatacaagt ggatggtcgt caatttatga ttagatgaat
    10141 ctgtcacagg aagcacaacg acaaatggtg ctgatttttc ttttacttaa tctgaagtct
    10201 ttatttccgt aaatcggtaa acagcgtcac gaccttgctc atctttgtcc gatgcactaa
    10261 aagaagcaat gccctcttcc tcgtctactg tttttgtcac attttctgcc agtatttttt
    10321 cactatcact tttttgtgct agttttcttt gtgcgtcttc cacactactt ccttctgacc
    10381 gcagcttgta aaattcatcc gagacatcat atatagcgaa agtaacgccg ttcaaccctt
    10441 ttactccttg aaagttatcc gggatattta ttccgtcgtt ttgactgata cttggcatct
    10501 tatcgctttc gaacattcga ttatgcaaag taaatgtaat aggtgattct ctagctgaca
    10561 cagtcggaga acctataaaa aagaccagta ggataaaaaa gagaacagca aatatatcca
    10621 cacgtacatt ttttgttttt ttcatcgctg ttcacctgct tttttcttac gatggataaa
    10681 ataaaagaag ccagaaaaaa taaagatcag tcctaaaaag acaaagggca gtagacctat
    10741 cccacctgtc gaaggaagca agagagtcgg attgatcgtc aacttagcgg tatctgttgc
    10801 gataatcgta taattattgt ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga
    10861 tccaatatct gtccggctgt taactgttag ggtataatcc aatgattcgc ctaagacaac
    10921 accggtaacg tggagatttc caaccgaaat cgagtgactt tgggcatcat aattaaaggc
    10981 agcgtcatcg acagtaatca ctgcgttagc tttatagatg gtataagttc ctgatgtgtt
    11041 ggctatatca gtaaatgaca acttgggatt tgctgctttg actttgtcta ttccgctatt
    11101 cgttagttta attgtatagc tgccagcatt aaccatattt tttgctgatt gtgcatctgc
    11161 tgtgtaatcc atactgctaa gtgccacttg tactggagta agcgaactag tgacaccctc
    11221 tgctagcttg acatggattg acgggacacc tgcttttcca tcatatgtct tactgccact
    11281 gagctctacc gcaccgctga tttgcttaat cgtatagaca tcagatgtct gcacgatagt
    11341 aaataaatag tttgttctgg cagctagttt agctagtccg ctttttgtca attgccaaga
    11401 cgtataggtt ccaatttcag taggcgcagt tacttcaact cccttttcat tcatcgcttt
    11461 gaaatcagtt ggattccatg tgatttgatc aatactgaga tcattgttta aaagggatgt
    11521 tgattctgtt ttatcattaa aggcatattt aacgatcggt acaacagaaa gaccatcata
    11581 gtacttactg ccatttgcat tgattgatac tgttgcattt gtcttataat ataggatgac
    11641 ttcagaaatc tgttttccat ttgcgtcctt tacgattaca tttccatttg tatctaaact
    11701 agctaaaaca ctcattgctg attggttctt tgtttctatc gctgaaacag ctgtaaaggt
    11761 atatccgttg atagtaggcg tattagtcgt caggtcttgt gcagcacttc cgtaagtaag
    11821 attcattatt ttagaaggag caatcatctg tccaaaatag tcttgatact taatagtgat
    11881 tggtgtttgt ttgcctgtat aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga
    11941 accgtttgtt tgtgcaaaag aatagcccgt tactgctgtt ggagtgataa ccactttgtc
    12001 acccacatgg attccagata atgtagtccc gtacgttccg atttttgctg ctccaccatt
    12061 gactgtataa gaaaaagttc ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata
    12121 ggttggaaaa tttgaaacat ttcccttacc atcaacgatc tgcccatttt tgatcgagta
    12181 ccccgtgaat ggaatatcat ttgctaactg tataccgatt gaaaaagtac tcatacctcc
    12241 tccggcagga agccctcccc atgttgaggg tgtaaaagaa taacttttat ccgcattggg
    12301 tgtaactgaa tcgatcgtat ttccagcggt agccaccccc atatagatac tttttgggaa
    12361 tgtgatagta gtaccattta ctgtggagcc aataaacaga ttatggatat ctggacggtt
    12421 atcaataaaa gcgattgctt tggttacgcc ttgtaacgca gttttatccg cactcatcga
    12481 tccgttaacc gcaacaatga ctccatcttt ggcatatatc actgtatatc catctggtat
    12541 ggtatgtccg ttgaaattcg cagtggccgc aacagtacta aatgtctggc ttctacctgt
    12601 ttctgaataa gctttctccc ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc
    12661 aatcagttga gtcgaggatg tggttgaggt tgtctcgttg ttgctagact gattggtgct
    12721 ttctatggac tgtatttcag aatagggtgg actatttgtc tcctctgtcg tttggttagc
    12781 aaaatttgac gtttctgtat aaacgtttac ataaatactt gataagctcc ctaagaaagt
    12841 aaaaatcaag atgatcgaaa tcataatggg atgtaagtat tttcttttag atacagcttt
    12901 gagtctagaa acaaaatgtt tttgacatat acgttggatt ttattttgag ttttcttaat
    12961 cattttcatc cctcctctca ataaa
//
LOCUS       DAHUWM010000078        12938 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHUWM010000078 DAHUWM010000000
VERSION     DAHUWM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.079"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(57..1166)
                     /gene="rpoD"
                     /locus_tag="NK788_001488"
     CDS             complement(57..1166)
                     /gene="rpoD"
                     /locus_tag="NK788_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCH6769823.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1190..3031)
                     /locus_tag="NK788_001489"
     CDS             complement(1190..3031)
                     /locus_tag="NK788_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCH6769824.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3213..5528)
                     /locus_tag="NK788_001490"
     CDS             complement(3213..5528)
                     /locus_tag="NK788_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6769825.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5713..6573)
                     /locus_tag="NK788_001491"
     CDS             complement(5713..6573)
                     /locus_tag="NK788_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCH6769826.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6632..7153)
                     /locus_tag="NK788_001492"
     CDS             complement(6632..7153)
                     /locus_tag="NK788_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCH6769827.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7431..8192
                     /locus_tag="NK788_001493"
     CDS             7431..8192
                     /locus_tag="NK788_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6769828.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8218..10809
                     /gene="mprF"
                     /locus_tag="NK788_001494"
     CDS             8218..10809
                     /gene="mprF"
                     /locus_tag="NK788_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCH6769829.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10997..12184)
                     /gene="tuf"
                     /locus_tag="NK788_001495"
     CDS             complement(10997..12184)
                     /gene="tuf"
                     /locus_tag="NK788_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCH6769830.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12552..12908
                     /locus_tag="NK788_001496"
     CDS             12552..12908
                     /locus_tag="NK788_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6769831.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4217 t
ORIGIN      
        1 cttttaaaat aaaccaaaca gttttactga tttctcttct ggtcaatagt ccacgcttat
       61 tctaagaaat cttttaattg ttttgaacga gaaggatggc gcaattttct taatgcttta
      121 gcctcgatct gacgaatacg ttcccgagta acaccaaata ctttccctac ttcttccaat
      181 gttctcgtcc ggccatcatc taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca
      241 gtcaatgtat ctagtacatc ttccaattgt tcttttagta actcataagc tgcgtgttct
      301 gctggacttg ttgcttcctg gtcttctatg aaatcaccta agtgagagtc atcttcttca
      361 ccgattggcg tttcaaggga aacaggttct tgcgcgattt tcaagatttc tcggactttt
      421 tctgttggaa gatccatttc cgcaccgatt tcttctggcg taggttctct tccaaggtct
      481 tgcagcaatt gacgttggat acgtatcaat ttattaattg tttcaaccat atgaactgga
      541 atacggatcg tacgtgcttg gtcagcgatt gctcgagtga tggcttgacg aatccaccat
      601 gtagcatagg ttgaaaactt aaatcctttg cggtagtcaa atttttcaac tgccttcatc
      661 agtcccatgt ttccttcttg gatcaagtca aggaactgca tgcctcgtcc tacataacgt
      721 ttagcgatac ttactactaa acgaaggttt gcttctgcca gacgctgttt cgcttcttgg
      781 tcgccttctt cgattttcaa tgctaacgct acttcttctt ccgcagttaa aagggagaca
      841 cggccaattt ctttcagata catacgaacc gggtcattga tttttacacc ggttggcgca
      901 gaaagatctt ctgtttttgc ttgttcagct tttttctcag cactcttcaa gctgtgaatt
      961 gatggatcac cattttcatc caccacactg atacctgcgt cttcaatctt ttggatcaat
     1021 ttgtccattt catctgcatt caaagtgaat ggtgtagcaa gctgattgga taaatcatca
     1081 tatacaactt gtcgtttggg tttgttttct ttgatgaaag ctgctactgc tgcttcatac
     1141 gtttttttat ctgttccatt tgccataaaa gaaggccccc cttgagtagc tatttggctt
     1201 gttttaactg tttcgttaaa ttgatgattt caatcgtcaa ttcaagctca agctgtttgt
     1261 ttccagttcg tctggcttct tgctgcattt gtttttttaa tgttatttca tcagctaagc
     1321 cagatttgct gatcacatct agcaagtctt ggatttctcg tggcgaactt tcttctgcca
     1381 ccggtaaata ttcgatttcc accaccaacc gccttaaatt ttcatcttgc aaaaagtcta
     1441 aaaactctgc aaggttgaat tcataatgtt gtgagacata tgcatcgaat aaaaaataaa
     1501 cttctgcata tgcatcatga ataaactgaa attctcgttc ttttaaaata ttacggacag
     1561 aaacttcttt aaatgcacga tataatagaa tctgctctgc cttttctact tgcgtcaaag
     1621 gtctcttttt ccgtgcagtt tcttgtctcg taacctgagt gtgttgactt tcctgaggtt
     1681 cttgacgatt ttgctgattt gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc
     1741 tgccgatctg aaactctgtt gacagttgag atagatacat atcttgttcg attggtgaaa
     1801 caactcggct aagttcaact agtaattctt cgaggtattc gatcttgtct ttttcattat
     1861 ctaaattttt cccttgtttt aaataacgtg atttaaaagt aaagactgtt tcgcgcccat
     1921 gagccactaa ttcttggaaa gatgcttctc catacttacg gagatattca tcaggatcaa
     1981 gcttttctgg aacgactacg acagaaagtg ctaatcgact gttttgccga agcaattcaa
     2041 tccctcgatt ggttgcttca aaacctgcgt tgtctccgtc atagcaaaag accaattctt
     2101 tcgtcagctt ttccatttgt tggatctgtt gagctgttag acttgtcccc atagaagcta
     2161 atccagtttt gataccagcc tgccatgcag caatcacatc cataaaccct tcgaacaaca
     2221 aaatttctcc gtttttccgg atatcgcttc ttgctttatc aagattaaat aaaacttgtc
     2281 ttttattgaa cagttctgtt tctggactat tcaagtattt gggctgatct ttttcctgcc
     2341 ctttttcagg tgcaagccag cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa
     2401 gtggaaacat cactctttga taaaaacgat cagacatacg gccatcatca tgcgtgacaa
     2461 acaatccaga gcgttctaac aattcatttg aaattgattc attcttgaat acttgctgaa
     2521 gaaaagaacg ctcattaggt gcaaaaccta tgttgaattc ttcaattaat tctaaagaca
     2581 acccacgact ttgcaaatat tctaaagctg gctgtccagc ttttgtatgc aatagcatgt
     2641 gatgatagat ttccgctgct ttttcatgag cagcaatcag tttccccgta gcagattcag
     2701 gattttggac agctatagta cgctgacgcc attgatcttc taacggtatc tgttcgaagt
     2761 ctgccacttt taccactgct tctggaaaac ttagaccttc tagttcttga agaaaagtaa
     2821 acacgtttcc gcctttacca caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag
     2881 aaaaagacgg tgtacgttct tcatgaaaag gacacagccc caagtaattt ttaccagact
     2941 ttttcaactg gacatattgt ccaatcacct caacgatatt cgtttgactg cgtactgttt
     3001 cgataacctc ttgaggaatc cgttgagcca ttttttcacc ccctgctcta ttgatatctt
     3061 ttgggtttaa cagaagatat ttcaggagaa taaccggtaa aaaatcgcac taactgaaat
     3121 cagtgcgatt ttaaaggaca caattataca tgtttgattg tatcatattt cattgctttt
     3181 tcaagtataa cacatttcta tctcattaag catcaatttg ctactctttt cagtaactga
     3241 ttttgaatat cagccttcca gtacaatact cgaagaacaa tatttacgag catgaataca
     3301 aagaagtatc ctacgctctc tcccaaccct aagtttactt ccaaaattcc atggaacagc
     3361 attgcagcta catatatccc tacgatcgtc gttactgcta ggaagatgcg caatgggttg
     3421 aagggataac aagcttttac aactgccatg caactgatcc caatcagcaa gtaatacatc
     3481 aaagtagtag tttcagcatt tccccatgcg aatgtttttc caagtagata caccgcaatg
     3541 atattcacta ctacaagcaa ggcatttggt aaagcattaa taagtgctgt aggcaaataa
     3601 cggtaagtga tttttctttt atcttgttca aaagataaaa agaaggaagg atacccttca
     3661 atcgctaaat caatcagcgt gatttggata gggataaacg gaaatccaat tgctgtaacg
     3721 gcacatataa tcgataaaat aaatgaataa atcgttttga taaaaaagat gcctgagact
     3781 ttcgtcacat tgttgaccac tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa
     3841 tcagagtcca acaataccag attcgcaatc tgacgtgttg ctccatcacc ttctgccata
     3901 gcaatgctgc aatctgcttc tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt
     3961 gcaaccgttc tgccgctttc tttcaattca tttaccagca atttcttttg ctgcggactc
     4021 actcgtccaa aaaccgtata gcgatgagca gcttctcgca cttccatttc ttcagtaatt
     4081 tgagaaagat cgacatacgc atcataattc ggtaatcctg cacgtctagc gatattggag
     4141 acagtaaaag gattgtctcc agaaatcact ttcagatcca caccttcctc atgaagataa
     4201 gctagtgttt cttgagcgtt ttttcggatt ggatcatcga tttcaaacag agcaaccggc
     4261 tcaacaaatg ctggtttttc ttcgttcaat tgctgatttg gagcgatccc caacataagt
     4321 acgcgatagc cattcgcttg ggctgttttt agactgtccg gaagccgatc ttcttccacc
     4381 aaacgttcag gcgctcctac gtacacgact cccagttctg gaaattccat cgcgccccat
     4441 ttgcgatcag aagaaaatgc gactttgttt gtcacatcat accgattaga aagctcaaaa
     4501 taatctctca tagcccgcat agtgatgttg ttatcactgc tttctgccag atacgtcccc
     4561 ataatttctg ggatggcttt ctcgtacagc ggatgtagtg cttctacccg ctggatactc
     4621 attttccctt cagtaatggt ccctgtttta tccaagcata atgtatcgac atgggccagc
     4681 gtttcaattg aatacatgtc ttgaacaagg attcgttttt ttgccagttt ggttacccct
     4741 gtcgttaatg caatacttat tagcaatacc aaaccctttg gaagcatgcc taacaaagct
     4801 gctgcagatg caataacaga aattttgata gaagcatcgc ggatgaacag tgcttccaaa
     4861 aatagtatga tccccaaagg aataatcacg aaactagtga atttggatac tttgcgaatc
     4921 gaatctacaa gttcagaacg aatagctttg tgagtctttg cctcgctagt caagcggaca
     4981 gcataattat ctgcacctac tcggataact tcagccatga cagaaccgct tgtcaaaaag
     5041 cttccagaaa gcaattcagc tccagtttct ttgacaatca aatcagattc gcctgtcaaa
     5101 agagcttcat ttgcttctgc ccgtccagat ataacctgta aatcggctgg tatctgttct
     5161 cctgctccga tttttaccaa atctcctaat accagctctt tagtagaaat cgattgttct
     5221 ttaccgtccc ggatcactag tacttctgat tgggaaacaa tcgataattt ccgtacaaga
     5281 tttcttgcat gtatttcttg ataaattcca ataacgatat tgacgacaat aattgctaaa
     5341 tacgccatgt tggaatacgc acctacaaat aacagacaaa tcccaatacc aaagttcaga
     5401 aaattgaata acgtcaatat attatcgctg aatatttgtt ttgtactttt tgatgcatct
     5461 tcctggtagt cattttgctg gccagaagcc attttctgaa ctacctcttg ctgtgtaagt
     5521 ccattcattt tctttttctc cattttccgc caactttcaa aaataatcac gtgtttaagt
     5581 ctagcgtgtt ccaagattaa aaacaacttg tttttataaa atttatagaa aaacacacga
     5641 atgattttac aaaaaagatg ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt
     5701 atcaatctat ctttacttat attgttcctc caaacggctc atccaccaac ctagtgctgt
     5761 accggctata aaaagaacag gacaagctag taccccagca actcccaatc ccgcgtaatc
     5821 ggtaggaatg atcataatcg ttgaagcaaa aactacacct aaaatgaaat ggaatagctt
     5881 gctatatgcc tttgagaaaa tgtaatccat gatttttgac aatccaagaa cacaaagaat
     5941 cccaccaatt ccgatcggca caatcacttc aagtcgtact tctttaaatc cgtcagccat
     6001 cgctttatac aatcccatat acaccaaaaa attagaaggg ctcaatcctg ggacgatcat
     6061 acccaaacca atcaatccgc cggcaatcag ccatgttcca aaattctgat cgacttgccc
     6121 gtcaaataat tgagcgccgt ataataaaaa caaataagcc agaacaaaag taacaaccat
     6181 gatgatcaca tcagaagtat ctcttccttt tttccctgct tctttccata acgcaggaac
     6241 agttccagcg atacatccga caaaaaacca taatatcgtt gtttcatacg cacctaataa
     6301 aaaactgact aaaaaagaaa gtagaaagat gcctgtcact ccgcctagac caacaggcag
     6361 aaaaaacaaa acattttctt tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg
     6421 ctcatacatg ccaaagacag ctgctaatgc accaccgctg actcctggca agataaatcc
     6481 tgaaccgata aacattccct tcacaaaccg taacatccag tctttggttg tgtaccccac
     6541 ttcttgattt tccatttttt gccttttttc cactatttgc gcactccttc tttcatttaa
     6601 ttttattcat gattgaacat ttcctgctat tttacttaaa gactttttca taggtctcta
     6661 ttattttttc attcattttg acaatatcag gataaaacat gttacctcca ttatgataga
     6721 gatagccgcc attgtaaagc aaggactgga tccgccaata tcggtattgt ttcccttcct
     6781 catttcctaa aagtgggctc agcacatctt tagaatacgc ttctgctaat tctactgtgt
     6841 tttctccgcc atgatttttt acataggcaa tataatcttt cccaaaatta tatgcttgga
     6901 cagctgtcca taaatcgcat ccttgtttct ttgcttctac tatcatttcc gccaaatatt
     6961 tgacaccttg atcaatactg tcagctggat tatcaatgat ctcagcttct ccatgcagac
     7021 tttccgagct ctgcatgaga tctgtccctt ttccttttga ttcggtcaat attatcgcct
     7081 tgatcaaatt ctcgtagttt tccatatgat aattttttgc agctgttgtt atttctattt
     7141 gatagctttc cactcgtttt accgaacgat aagttcttcc tcataaaaac aacaccgcac
     7201 caataaccgt cagaatgatc atgtaaaaaa gaatacggat aatagataat cgttttcgct
     7261 tcatttcatc acctagttat tttgacatct ttttttatct tactatttct ttatcttctt
     7321 ggctatttct tccttgattt ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt
     7381 ttgacatctt acgaaaatga tcgttgtttt tcagtaaaaa caattgccca atgactgata
     7441 taatcgttat actttacaca gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa
     7501 tgccagatgg gagcgaaatt tactatgaaa agagcggtca aggcttccct ctctttttac
     7561 tgcatggcaa tgacggcagc ggccgctttt tttcagaaca ggtccctgta ctcgaacgct
     7621 actataccgt gtatctagta gatagccgag gacatggtcg ttcgacaaac gaagcatcta
     7681 tgttgaattt tcagttaatg gccgaggatc tgaatacgat catgttgctg gaaaaaatcg
     7741 atcaggcaga ttttcttgga tttagtgatg gagcaaatct agcactggta tttgccagca
     7801 gcttccctaa aaaagttcat cgattgattt taaattctgg aaacacgctt gttaaaggcg
     7861 ttcgtttttc tgccagagtc atcagtaata ttcattatgc atgggtatgg ttactctctt
     7921 tatttcgccc aagtcttcgc aaaaatcttt tagttatcaa acttttgctg catgacatcg
     7981 ggcttacaga aaatgattta aaaaaaatca actcccccac tttgatcatc gtgggaaaaa
     8041 aagatgtaat caagctaaaa cattctttgt atatagctaa aactatacca aaagcttcct
     8101 ttgtgcttgt gaaagaacaa ggacatgaat tagctagaaa agatcctgaa cgcttcaatc
     8161 gagaagttct tcaatttctt tctgaaacat aactattagg aatagaaggt gatttatttg
     8221 ttaaaaaata ccatacaatg gctgaaaaac cacctaggac tgttcaaaac aatctttttg
     8281 atttctgtca ttgtcattat tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc
     8341 cagcaattag gagaaacgtt tgccactata cctgtatgga aaagcggact aatgctattg
     8401 attggtctta tctctgtgtt acctatggtt ggttatgata ttattttgaa tcggatgctt
     8461 gaacaaaagc agaatcctcg ttacctgttt gagacaagct ggctgatcaa tacgatcaac
     8521 aatattgccg gtttcggcgg gtttgttagt gtggggcttc gctctgaact atatggacaa
     8581 aagaaagaga gcagaaacgt gatccaagca ttatcgaaag ttttcttgtt tttgatggct
     8641 ggactttcta tttacagtct gctttctttt ttacttgtag tcttcacgcc agttgctcct
     8701 tttttgaagc aatattggat atggttgatc ggcggcggtc tatattttcc tgtcgtcttt
     8761 ttatttacta ctttcaaaaa gaaaggattc cttggtggta cctcacttaa aactcaagga
     8821 cagctattgc tagtttcatt gttggaatgg accggtgtcc tgtttagttt cctttctgtt
     8881 ggttatttaa tggaaatccg gattgatctg tggcaaacaa ttccattatt cattgctgct
     8941 tcagtgatag gaatcgtgtc tatgattcca ggagaaattg ggagtttcga tgtaatgatg
     9001 attatcgggt tgtccgctat tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc
     9061 tatcgcctgt tctactacat cattccgttt ttgattggaa tcgtcttttt cttcaaaaat
     9121 atcggttcca cttttgatca gcgttattct ggtatcccta aacaattagc aacagaaatt
     9181 gcgcataaaa tcgtggttgt cttattatac ttttctggaa tcatgctagt actttctgcc
     9241 actatcccgc aagcttttac agagtttcgc tggcttcata gtttgaaccc attgaaattt
     9301 cattttatta tccaatttcc tagtatacta ttgggatttc ttttaatcat tatgggcaga
     9361 gggatcgcag cacgtgtgaa acgagcatat ttgcctacaa tcttcttgat tgttctagct
     9421 ttactttatg ttttattaag tgattttagt ttcacaccgg tggttttctt gtctattctg
     9481 cttctgatca ttcttgcttc taaaaacgaa ttatttcggg aacaattgat ctattcatgg
     9541 gagtggcgta cgatcgatgg aatcattatt ggtgcactgt ccttgcttta catcgttatt
     9601 ggggtataca atctgccgga tttccctcat cggcgtcacc attttatttc ctttttctta
     9661 tttccttctg aaaagatttg gttttcggga ttgttggcca tcatcgctgt tagtttcatg
     9721 atcgttctat ttgttcattt ccttcaagga gaaaagaaac agattggcga agcctttaat
     9781 gaagaaaagg ccctgaagat ccttacaact tatggaggaa actccgatag tcaattgatc
     9841 ttcttaaaag ataagcggat gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc
     9901 caattcgctt gcttcaataa taaatgtatt gtaatgggtg atccttctgg taagaaagaa
     9961 gattttccag aagcaatcga agcttttatc gaagaaacag atcgactatg ctatttacca
    10021 gttttctacg aaacaagcga agagattgtt atgattttgc atgaattcgg ctacgatttc
    10081 attaagatgg gggaagaagc ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa
    10141 atgaagggca ctcgtgctgt cttgaatcgg atcgaacgag aaggatttac atttgatgtc
    10201 ttacaaccac cattttctac tgagcaaatg agtactttca aaaatatctc tgataattgg
    10261 ttgggttcac gaaaggaaaa agggttttct ttaggctttt tctctgaaga ttatctacaa
    10321 agagcgccta ttgcagttgt caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc
    10381 atgcctactt acacgaacaa taaagtagga acgattgatc tgatgcgata tgattctaaa
    10441 aaagcacctt ctggcagtat ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa
    10501 gaacatatcc aatggttcaa tttagggatg gcccctcttt caaatgtcgg aacttccaga
    10561 aagagttttt tacaggaaag gattgcatac ttggtttacg agtttggttc tcatttttat
    10621 tctttccatg gattaagaga atataaaagc aagtacgcta caaactgggt ctcacgttat
    10681 actctatatt ctagagatag ttggattgca tatgttatga ttgctttgct gattattgat
    10741 aatgcgccag ttgagcagac acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga
    10801 aagtattaaa aactgcatac cgatagatga acactctcat gatgaataaa agagactgaa
    10861 gtaaaacgaa agttcatgct tctacaatct cttcttatac aattgaagaa caaagagcgg
    10921 tccaaacaga aatcctgttt ggaccgctct tttattttaa acatttttca ttaccaaaat
    10981 attcgagtcg cgtttcttat gcttcgatct ctgtaacgat acctgaacct actgtccgtc
    11041 cgccttcacg aattgagaat gtcgttccgt tttcaacagc gattggatgg atcaagtcaa
    11101 catcaatcgt cacgttgtca ccaggcatga ccatttctgt atcttctggc aatgtgattg
    11161 ttcctgtaac atcagttgtg cggaagtaga actgtggacg atagttgttg aagaatggag
    11221 tatgacgtcc accttcttct tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg
    11281 gagtaatgga acctggttta gcaagtactt gtccacgttc gatatcgtca cgttggatcc
    11341 cccgtaacaa cacgcctacg ttatccccag cttccccgta atctaacgtt ttacggaaca
    11401 tttctacacc tgttacaact gctttttgtg tttcaggttt gatccctacg atctctacct
    11461 catcaccgac acgaacagca ccacgatcaa tacgacctga ggcaacagtt cctcgacctg
    11521 taattgagaa gacgtcttcc actggcaata gtaatggttt atctgtatca cgttctggtg
    11581 ttgggatata ttcatctact gtatccatca attccataat agcagcttca gcatctggat
    11641 cgccttgcaa tgcttttaat gcggaacctt tgatcacagg agtgtcatcg cctgggaaac
    11701 catattcgct caataactcg cgaacttcca tttctaccaa atcgatcaat tcttcatcat
    11761 cgactaaatc aactttgttc aagaaaacaa tcaggtattt tacaccaact tggcgtgaca
    11821 ataaaatgtg ttcacgtgtt tgaggcatcg gaccgtcagt tgcagaaaca actaagattg
    11881 caccatccat ttgtgctgcc cctgtaatca tgtttttcac atagtccgcg tgtcctggtg
    11941 catcaatatg cgcgtagtgg cgtttttctg tttcatattc tacgtgtgcg gtattgatcg
    12001 taatcccacg ttcacgttct tctggagctg catcaatact agcatagtct tgaggatttg
    12061 ctaggccttt tttgcctaat actgtagtga tcgcagcagt taaagtagtt ttcccatggt
    12121 caacgtgtcc aatcgtacca atgttaacgt gtggtttact tctgtcataa tgttcttttg
    12181 ccataattta caaacctcct aaataatata gatcactgaa aaatttcagc tctcaccttt
    12241 ttattatagt ctttttttga ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga
    12301 ttcgaactaa gcttattata cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt
    12361 cttatacgcg aaaaaagtac cttttataaa taaaaaaatg tgttttttga gaaaaacttc
    12421 aagatagtcc ttaactttct tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt
    12481 cagctgttgc caacaacttt caatagacga aatcttctat ttcgttaata ataaggaaag
    12541 aggtgaagta catgaaaaaa ttaattggcg tacttgtcgt tttaggcata tttctcggcg
    12601 gaagttttgc agcttatcat tatttttacg gcggcgaagc ttattataca aaaatcacga
    12661 cgaatggcga aatgtcagct acccaagcag ataacggaga gaaattcacg acttatacct
    12721 acaagcaagc ggcttataat aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa
    12781 gagaacatcc attaaagatg aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg
    12841 tattaagctg ggaagaaata aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg
    12901 ataattagta aatggtctta aaattttatt agataaaa
//
LOCUS       DAHUWM010000079        12910 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHUWM010000079 DAHUWM010000000
VERSION     DAHUWM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.080"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(185..1633)
                     /gene="cls"
                     /locus_tag="NK788_001497"
     CDS             complement(185..1633)
                     /gene="cls"
                     /locus_tag="NK788_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCH6769832.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1785..2678)
                     /locus_tag="NK788_001498"
     CDS             complement(1785..2678)
                     /locus_tag="NK788_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6769833.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="NK788_001499"
     CDS             complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="NK788_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCH6769834.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3453..4554)
                     /gene="prfB"
                     /locus_tag="NK788_001500"
     CDS             complement(join(3453..4481,4483..4554))
                     /gene="prfB"
                     /locus_tag="NK788_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCH6769835.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4680..7214)
                     /gene="secA"
                     /locus_tag="NK788_001501"
     CDS             complement(4680..7214)
                     /gene="secA"
                     /locus_tag="NK788_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCH6769836.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7436..7987)
                     /gene="raiA"
                     /locus_tag="NK788_001502"
     CDS             complement(7436..7987)
                     /gene="raiA"
                     /locus_tag="NK788_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCH6769837.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8115..8834)
                     /locus_tag="NK788_001503"
     CDS             complement(8115..8834)
                     /locus_tag="NK788_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCH6769838.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(8831..10144)
                     /locus_tag="NK788_001504"
     CDS             complement(8831..10144)
                     /locus_tag="NK788_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCH6769839.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(10225..10545)
                     /locus_tag="NK788_001505"
     CDS             complement(10225..10545)
                     /locus_tag="NK788_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6769840.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(10653..10940)
                     /locus_tag="NK788_001506"
     CDS             complement(10653..10940)
                     /locus_tag="NK788_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769841.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            11067..11711
                     /locus_tag="NK788_001507"
     CDS             11067..11711
                     /locus_tag="NK788_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCH6769842.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(11771..12478)
                     /locus_tag="NK788_001508"
     CDS             complement(11771..12478)
                     /locus_tag="NK788_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6769843.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(12698..12868)
                     /locus_tag="NK788_001509"
     CDS             complement(12698..12868)
                     /locus_tag="NK788_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769844.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3561 a         2796 c         2236 g         4317 t
ORIGIN      
        1 gtgtctcttt taatagcata tattagctta tttcttgctt tccccaaaaa acaagagata
       61 ccccaaagtt aatttcgctc atgcgagacg ctttctccat ttctgacgta ctctcagaaa
      121 tggccccgtt agaaaaggac acgagatctc catctgttga tggttctcat gtccttttta
      181 tgttttaaag gagcggcgaa atcaatcttg ctaatccttc tttcatcttg atgattattc
      241 ctcggttttc atacatttct tttgtgagat gcgtagaaat tttcgagtct tcaaaaaaag
      301 cttttcttac acgtgaagcc attcgttcat cataaaccag tgtattgacc tcaaagtcca
      361 agcgaaatga acgaacatcg atatttgctg agccaactga aacaatgcca ccatcgataa
      421 tcatcgtctt tgcatgaata aatccattct catatgtttc gatccttgct ccatactcga
      481 ttagctctgc agcgaaagaa tacgttgccc agtaaacgag aagatgatct ggtttgttag
      541 gtatttggat atgaactttt acgcctgaaa gaagagccaa cttcagtgct tcatgaatcg
      601 atccatccgg tatgtaataa ggcgtttgga tcaatatctc ccgtttggct aacgaaatca
      661 ttttcaaata agtcattttg atttgttcat gttcggtatc tggtccgctt gtcacgattt
      721 gaacagctat atcaccagta gagtgaatgg acggaaaaaa tttgggagaa tagcctactt
      781 cttttgtatg ctgagaattc cagtccatca aaaaacggtt ttgcaaggta tacaccgctt
      841 ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa tttctttacc aatcctagat
      901 attcatttcc aacattgaac ccacctgtat atccgatttt cccatcaata actacgattt
      961 tacgatgatt gcgataattg atccgaggat taatatacgg aacaaataga ggaaagaaaa
     1021 atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa gctggaagaa acttgtgtgg
     1081 aaccccatgc atcaagaagg actctgactt ttacccctcg tcttgccgcc tctgtcaatg
     1141 catctcgaac ttcttcacct aatgcatcac tgcgataaat ataatactgg aagtggatat
     1201 ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa tttttcacga ccatctgtaa
     1261 ataaagtgat ttcattttta gtcgtataga gcgtactttc aaataccgtg agcatataga
     1321 tcaattgttt cacatctact tgtccagtag gcggatgagg ataaagccct cgcatcaatg
     1381 cctgtttgtc ttcttcaatt tccaggttct taccgatttt cccttggatc ttcaaatcaa
     1441 agattttctc tttggaaatc cctcttccta aaaaaatata taaaataaat cctacaactg
     1501 gaatgaacat caaaacgagc acccatgccc atgtttgagc agtttccttt ctttctcgaa
     1561 aaacgatgat aaaggaaagc aaaatattca taatcaaaag gatcgtaaaa aaattgtcaa
     1621 cgataatttt cacggcttca cctcttcttc taccaactta agaataccca agttgttttt
     1681 taaagtaaag ggaaaaaagc cgcttccttc tattctccta gccaataaaa aaagaaagct
     1741 ggaacgaaac tgtccaactt tctcatttta tttatattgt ggtattagaa tttcaagaaa
     1801 cgtctcatag aaatgatcga gccaagtgaa ccaatgatca caccaatgcc gaccattaaa
     1861 agattgattt tccaaacgat attttgtgga tgaatcaatg aataatggga acgtaataat
     1921 gatggattga tcaattggaa aaaccatgca taaccaaatg tgatgatcaa aatcggaaga
     1981 atcgcaccga tcagaccgat ccagccacct tcaaggaaaa acggccagcg aatatatcca
     2041 ttttttgcac cgactaaacg catgatttga atttcacgct gtctagacaa aatcgtgatc
     2101 cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca gcaatgccgc tgcaactaat
     2161 ccccatgtcc gaactgcacc agcgatttgg aaaatccgat ctgaggaagc tccaccgtaa
     2221 tcagaacgag aaacattctt caaatctgct gcttcttctg ctacttgttt tgtataagaa
     2281 ggatcagtag cacttactac atacacatca tataacgggt tattgtcacc ttcaaacaat
     2341 ttccaggaat cacccatttc gttttggatt tttttcaatt cctgttcttt gcttgaaaat
     2401 tcaacttttt taacatgatc taagtcattc aattcttttt ctagcttttt catctcttcg
     2461 tcttttgtac cgatatctac gaatacagag acatcaacgt ttccttctat atcttgagct
     2521 aatttagttg cgttcatgat aactgccatg aatacaccga ctaaagtcaa tgtgatcgtt
     2581 accgcactga ctgaagcaac tgtcatccag ccgttacgtt tcaaactctt gatactttct
     2641 aataaatggc ggaaaaaggt tctaatcatc gtatccatat tccccttccg cttgatctcg
     2701 aataatgcgg ccgttttcga tagcaattac tcggtgccga atcgtattta cgatcgtact
     2761 gttatgtgta gccatcacga cagtagttcc ttgggcattg atccgatcta ataatttcat
     2821 gatttcccat gagttttctg gatccagatt tcctgtcggt tcatccgcga taagtacttt
     2881 aggcgtattc actattgccc gagcaataga gacacgttgc tgttccccac cggataattc
     2941 gctaggaaag actcgaactt tgtgtttcaa accaacaagg tccagcactt ccattactcg
     3001 ttttttgata tcccgaggtt ttctgccgat aacttgcata gcgtacgcaa cattttcata
     3061 tactgttttt cttggtaata atttatagtc ttggaacacc acgccgattt ctctgcgtag
     3121 ataaggaact tcgcggtttt tgattgctat tagatcatga cctgcgacgt tcaaagttcc
     3181 ttttgaagct ttttcttctc gatacatcaa tttgatgaat gtagatttac cggctccaga
     3241 cggaccaacc acatagacaa actctccttg ttcgatgaca attgagagat tgcggatagc
     3301 ggtcgtacca tttgagtact tcttcattac atccttcatt tcaatcatgg cgttctctcc
     3361 atatcttttt cagtctttag ctattatagc atggcaatgt ttcaacatgc ttgcaaaatc
     3421 ttacaatttt atttcaattt aacaatgggt tcttattgta gatgcatttt taaatagccg
     3481 tcaataaata aatcaaggtc accgtccata actgcttgga catttcctgt ctctacgttt
     3541 gtccgatggt cttttaccat agaatatgga tggaaaacat aagaacggat ctgtgaaccc
     3601 cagccgattt ctttttgttc tcctcgaagt gcagctgctt cttgttcttt cttttccact
     3661 tctaattgat aaagcttagc ttttaacatc cccatcgctt gctcacggtt tttcaattgt
     3721 gaacgttgtg cctgactagc tactactgtt cctgtaggca agtgagtgat ccgtacagca
     3781 gattctgttt tattgatgtg ctgaccacct gcaccacttg ctcggtacgt atcgattttc
     3841 aaatcatctg gattgatttc tatatcgatc gtttcatcaa gctctggcat cacatctacc
     3901 gaacaaaaag aagtatgacg tcgtttcgct gagtcaaaag gcgagatacg tacaaggcga
     3961 tgaacacctt tttctgattt cagataacca aatgcgttgt gtcctttgat cagtagggtc
     4021 acactcttga ttcctgcttc atcacctgct tgataatcta gtgtctctac ttgataccca
     4081 tgctgctctg cccagcgtgt atacatacgt aaaagcatac tgccccaatc ttgggactcc
     4141 gttccgccag cccctggatg caattcaacg atggcgttgt ttcgatcata aggaccatct
     4201 aacaacatag ataattcata tgtcttcatt ttttcatcta gcgcattgat acgtgtttct
     4261 gcttcttctt gcatggctgc atcaggttct tcctgcaaca tctccaccat caattcgatt
     4321 tcttccagtt cttcagcaag agaatggaat tgatcataag tttctttatt tgcattgttt
     4381 tcactgatca ctttttgagc agcttcgcta tcatcccaaa aaccaggttc agccattttt
     4441 gcttctgctt ccgcaatgtc ttcttccagc tggtctaagt caaagagacc tcctgaagct
     4501 tgtgaccttt tcctgcattt ctgtcaggag atttcgaatt tcggatattt ccatgttttt
     4561 tccttctttc actatacttt tttcattata tctagtctaa cagaatcagc aaataacaga
     4621 aagaaggtga gacgaattct taaaaaaaga atttgtctca ccaatcctgc tgtcagaaat
     4681 tagtttgcat tttttccatg acagttctta tattttttcc cgcttccaca aggacaagga
     4741 tcatttcttc cgaccttgtt cacatggatt ggctgttttt tcgcgctagt attactattt
     4801 tctttggcat ctccgtcttc tgagtgagaa gctgttcctt gagaaacctg ttcacgctgt
     4861 acgttttgac ggatctcagc tttcataaag attcgagtga cttcatactc gatggcgcct
     4921 accatatctt caaacatctt atagccttct gtttgatatt cgactaatgg attgttctgt
     4981 ccataagcac gtaagccgat tgactgacgt aattgatcca ttgcatcaat atggtctgtc
     5041 catttagagt ccacaacacg caagatcacg actttttcaa attccaacag ttgctctgga
     5101 ccatttagtt gagccgcttt ggtattaaat acttcttgtg ctcgttcgtt tagatactct
     5161 ttgatctcct gagctgtttt tccttcgata tctgcaagag taatactatc ttcatggaca
     5221 agagtactgc cggcaaaatc gatgatactt tccaaattcc aattttcttt ctctaattgt
     5281 gtatgcgcat ctactacacg actgattgtc cgtttgacca tgttcattag tggcttggat
     5341 aagctttttt cttccatgat tacttcttgt cgctgtttat agattacttc acgttgttca
     5401 cgcatcacat catcgtattg taagacgttt ttacgtgtgt catagttatt tccttctaca
     5461 cgtttttgtg cagattctac ttgtttcgtc agcatcttgc tttggataac agcatcttcc
     5521 tcaccgatct tcatccgatc taagaaagat ttgattcgtt ctgaaccgaa tcgtttcatc
     5581 aaatcatctt ccaatgacaa gtagaactgt gacataccag ggtctccttg acgtcctgca
     5641 cgtcctcgaa gctggttatc gatacgacga gattcatgtc gttctgtccc gataactgct
     5701 agaccaccaa gttctcttac cccaagtcct aacttgatat ctgttccccg accagccatg
     5761 ttggtagcaa tcgtcacagc acctttttga ccagcattca tgatgatctc tgcttcttta
     5821 aagtgatttt tcgcattcag cacttcatga ggaatacgtt cttgattcag catatctgat
     5881 aataactcag atgtttcaac cgcaacagtt ccgactaaca caggttgacc tttgcggtag
     5941 cgatctttga tatcttgcac gacagcgtgg aacttgcttt gtaatgttgg gtaaagcaaa
     6001 tctggacgat catcacggat aaccggacga tttgtcggaa tctggtatac ttggatgtta
     6061 taaatttctc tgaattcttc ttcttctgtt ttagctgtac cagtcattcc cgcaagtttc
     6121 ttgtacatac ggaaatagtt ctggaaagtg atcgtcgcca tggttttcgt ttcgtcttcg
     6181 atctctacgc cttctttagc ttcgattgct tggtgaagtc catctgaata acggcgtcca
     6241 tccatgatcc gaccagtgaa ctggtcaaca atcagtactt ttccgtcttg gacaacataa
     6301 tcgatatcta aaagcatgat atagttggct ctcaatgctt gatccaagtg atgggtcaat
     6361 gcagtatttt caatgtcgta taagttatcc aaaccgaatg tttcttctgc tttttcgatc
     6421 cctgcttcgg ttaaactgat tgtttttgat tgtacatcga ttttataatc ttcttcttct
     6481 ttcaaacgtt tcacgaagtt gtctgctctt gtatataaag ctgtggattt ttctgcttgc
     6541 cctgaaatga tcagcggcgt tctcgcttca tcaatcaaga ttgagtcaac ttcatcgaca
     6601 atggcataat tcagcggacg ttgtaccatt tgatggcggt aaacgaccat attgtctctc
     6661 aagtagtcaa atcctaattc attattcgta ctataagtga tatcacaatt ataagcctcg
     6721 cgtttttcat ccgaagattt tgagttgata ttcaacccca ctgttaatcc taagaaatta
     6781 taaagttcgc ccatttccgt cgagtcacgg gttgctaaat attcattgac ggtaactacg
     6841 tgtacccctt cgccagtcag agcatttaga tatactggca tcgttgcggt caaggtttta
     6901 ccttcaccag tacgcatttc agggatattt ccatcgtgaa gcacgattcc acccatcaat
     6961 tgtacgtggt gtggatataa ccccaataca cgttttgctg cttctcggac gactgcaaaa
     7021 gcttccggaa gtaattgatc taatgtttct cctttttggt atctgccctt gaattcagca
     7081 gtttttgcct gtaattgttc atcagttaat gctgccattt ggtcagcgta tgcttcaact
     7141 ttttttgcaa tgctatctaa acgttttagt tctttcttat cgttttcgat aagtgaacga
     7201 atcaaattgg ccatgcgttg tgtaatctcc ttttccttct gtgtattatc cagactatgt
     7261 agactttact tttcatttta tcattagtta tagataattg aaatatctta agcgtattaa
     7321 agtaagaact tcataattta ttcatatttc atctaaaaga ggaggaaact caaaaaaact
     7381 tgcggcagct gtcagtagct gctgcaagtt ttcgttgtta tttaagtaag gctgatcagt
     7441 ctgtttcaat cagtccgtat tttccatctt tacggcggta aacgatactt gtaccatttg
     7501 tttctgcatc ttcaaaaata aagaagttat gtcccagcat gttcatttgt aagactgctt
     7561 cttcactatc cattggtttc aatgaaaggc gtttcgtacg tacgatatct aattcagaat
     7621 cattttcgtt ttcgtcctct tcattgaata aagcagtagc atctgcagta ttcatgcccg
     7681 tttcacggga tttacgatta atttttgttt tgaatttgcg gatttggcgt tccaatttgt
     7741 caacaaccaa atcgatactt gcgtataaat ctggagaagt ttcttcggca cgtaatacaa
     7801 gataaggtaa tgggatcgtt acttccactt ttgctgtttt ttctgtataa acttttaagt
     7861 tcacatgcgc cgttgcatcg ggtgcgtcac tgaaatagcg ttctagtttg cctacttttt
     7921 tctcaacgta gtcgcgaatc gcttcagtta cttcgatatt ttcgccgcgt acattatatc
     7981 taaacataaa aattacccct ttcgtctacc acaatcaaga aggagaagga ccactctttc
     8041 cctcttctta attctattat atcgaactct aggttatttg caaattaaat cgctatcaaa
     8101 tttccacatc taattcatct tgctaaagaa atggttcgga tgattttcgg ctgtgcgacc
     8161 tgcaaacaat ctgctgcgtg gaaaagagtc tgtcctgttg tatagacatc atcaataatc
     8221 aatatttttt tattttgtat cttagaaaag gtgactgcaa gttcaaaagg ttgaggcatc
     8281 tccaatcttt cctttcgcgt gttttttgct tgagccagca gatgcttctc tcttttgagt
     8341 agcatctgaa aaggaattct tgctgctttc aacatctcgc ttacttgatt gaatcctctt
     8401 tctgttcgcc ttttttcaga taaaggtatt ggacaataga tgtatgtcgg gtattctttt
     8461 tttatctgcg tccatttata tgcaaaggtg cctgctagtc ggatatctcc cataaacttg
     8521 tattgattga gccaggcctt catattttca tcgtacacaa aaaaggcacg gtgctgaaaa
     8581 tcataggtcg gatatttctt cttccattct tgacagtcac tgcagtaaac gggaatagac
     8641 tttactttct ttttcgtact ctctagattg aaatggattg gtttttgaca gccagaacag
     8701 cctcttgttt ccaataaatg aaattgtgcg cgacaaactg aacaagtctg aggtaatctg
     8761 tatttcgaaa caccaataat ctcgctgaat gttaaatttt ggacgatatc tttactgcag
     8821 caactgcacc tcacagcatc ttcttccctt cttcattcat ttctttgatt tcctgacaag
     8881 cttttcgaat agctgcagtt ttttcttcat agaaaaagta tacttgactg ttcgtataag
     8941 gccctttacg atcggctctt cctgcgattt gtaccaaaga agatttagta aaaaccggat
     9001 gatccgctcc taaaacgata acggatattc tttcaaatgt gactccacgt tcaaggatca
     9061 tcgtggttaa cagtatgtca tattttgctt ctcgcatttt ctggactttt tccgcccgct
     9121 gattgtctct tgaagaaaca tctgtgatct catatctcgg aagagcctct tgaagtgtgg
     9181 ctttcaaacg atgcatcatc gtgatacttg gacaaaataa aaggatatgg ttttctttaa
     9241 ttaattgatg aatgatccgc accaaagatt ttatcttctt cttgttcttg catttttgtc
     9301 gccaattatt catgaaaacc aagacgggtt caggaagtaa acgccgatga aagcgcaaag
     9361 caagtttgtg gatgtcaaac gtctgcttga tttcctttag taacttttta tcaggtgtgg
     9421 cacttaagta gatgaatcgt ccgtctggtt taatcgctgt ttgcacagca tgagctaatc
     9481 ctgcatcccc gttataagga aacgcatcga tctcatcgat cactattaaa tcaaaggacc
     9541 gataaaaacg gtatagctga tgaatcgtac agataacaaa agaagtaaag gtataagccg
     9601 ttgctgtatt cccatggagc aacgtgattt tttcttctgg aaaaacataa cgatagcgta
     9661 aaaacaattc attacaaaca tcgactcgcg gtgtacaaat cgctactctt cctccagctt
     9721 ttaaagtttc cactaaaata ggataaagca tctctgtttt ccctgcacct gttacagccc
     9781 aaataagatg atgtcttttt tctgaatggc aaagtattct ctttgcgatt tccgtttgtt
     9841 tttcagtaag ctgtcctgtc caaaaaaaga cgacttctcg aggttttgct tcaggctgat
     9901 caaacaaata taaatcttgt tgagtgtcgc atcgccctaa atatagacaa tgtgtacaaa
     9961 aataaccgta atctgcaagc tgtccatcaa tgacggacgt cctcattcca cagcgatgac
    10021 aaagaatcca gttgcccaaa cgttccactg ctgcgatctt ctcataattt ggtaaattta
    10081 cctcctcaca gtctattgct ttaatatctc tcgtcagaag ccttctaccc caaaaatgat
    10141 tcatattatt cgccctaaaa aaatatacga aaaaacaagc gatctatttt ttggatcgct
    10201 tgttcgatag ttatactgtt ctatttattc acctatgagc gtgattgctt gatctaacac
    10261 tttttcgcca ttcatcatac cataatcagc catattgatg acgtctaaag gaatgccttt
    10321 tggttccaga cgtttttcaa aatcagcttt cataaagcga acttgaggtc ctaataacag
    10381 tacatctaca tctttatttg caagattgtt atctgcgtca gaagcggaca cagcgaaaat
    10441 atccgagtcg attccttttg cttcagcagc tttttgcatt tttgttacta gtaaacttgt
    10501 gctcatacca gccgaacata caagcatgat tgttttttta gccatacttt tctctccttt
    10561 ttaaaacaaa ctacgcaaac gttttcttcg cgtctgttac tctattatat aaaaagaaag
    10621 aaatttgtca acgttttcta aacgcggttt atttaatcaa atagccaact agatttcact
    10681 aatttattga acacgacata ttctttgtcc aaattgatga caactacttc atagcctgca
    10741 tttatccagc cataggcacc tttttcaggg tgtttataat catttgccca ccaatctgta
    10801 ctttcccttg ctgttttggg aagtccgtta ctgggaaaca gcagttcgtc aaattgggta
    10861 aaggtcaacg ttacttgaga gccgccattg ttcttcaaat aatgcgtaat tgcatcgtac
    10921 tttttcattc gttcctgcat ccccagatcc acctaccttt gtctttatta tcgcatacta
    10981 cagcgattgc gcaaccgtta cagtgttgaa acaacctaga actttattta tactgaataa
    11041 agaaaatgaa aaaaagaggt gacaagatga tcgagcgcta tcgtacaata aaagaaaatg
    11101 gacaaagtga gattgagatc aaaaaatcac gctttatctg ttctcttaga cgaatagaat
    11161 cagaggacca agcaaaaagt ttcattcaag aaataaaaaa agaacattgg aaagcgaacc
    11221 ataactgcag cgccttcatc attggagaaa aaaatgaaat ccagcgaagc agcgatgatg
    11281 gagaaccaag tggaacggct ggcgtaccga tgttggaagt cctcaaaaaa aatcaactga
    11341 tcaatgtagc tgcagtagtc actcgatatt ttggaggaac gaaactcggc acaggcggat
    11401 taatacgagc atacactcat gctgtctctc aagcattgtc tgacattgga atcgtagaag
    11461 gtaccttgca acaggagatc tctgtgatga tcacgtatcc gcaactagga aaattacaga
    11521 attatcttga actccaacat ttttttataa aagaaatcca atatactgat caagttagcg
    11581 tcatctgtgc tgtagatgaa aaaagtgctg accactttaa agaatccatc attgagctgc
    11641 tgaacggaca agtccaattt gaagagggtc ctgtttctta tcacgagcaa ctggtaactg
    11701 atcatatata aaaacaggtg attctgattc tcttttattt ctaagtaagg aatcagggat
    11761 cacctgtttt ttatttttcc aaataaaact caaaacgatt tcctacgtat tgcgtacgca
    11821 catactcaaa cggcgtaccg tcttcaaaat aagaaatctg gcgcaaacga agaactgcat
    11881 ctcctcgttt tacctctaaa tagtcagcaa ttttctcaga agccaacaca gcagtaatcg
    11941 tttgattcgc tggaccgatc tttttcccgc ctttttcttc tagcgtgcga tacaacgatt
    12001 cagtaatctc tgctttgctg tagccatcga tcagcttttg tggcacactg gcaacttcaa
    12061 aacaaatagg gacatcatct gcgaaacgaa tacgttccat tcgcaagatc gtgtcatctt
    12121 tactcaagtt tagtttttcc atttcacttg agcttggcga agtcaagaaa taagagattg
    12181 tccgactaga cggtactcga ttttgcgatt ccataatttc tgtaaaactt gttgcaccag
    12241 acattttttc ttgtactttt tctcgcgcaa catatgtacc cgaaccgatc tttctttcta
    12301 agattccttc atctgccaag ttttgaattg cttgtctcaa cgtcatacgg ctgacattga
    12361 atttcactgc taattctctt tctgaaggaa gacggtctcc gactttccat ataccttgtt
    12421 cgatttcatt ttttaattgg tcatgaatct gtatatatac aggtaattga ttcgacataa
    12481 cgtacacccg tcctttcgtt tcttctatta taactggtat atacctgatt cacaactgaa
    12541 aaagataaag gtttctatcc atcaacagag gatttagaca cacatcgttc ttcctactcc
    12601 tattgtcaga aacaattttt tagatatgtt caacctagaa aataacaaaa gaccctaaaa
    12661 tcgatgaaaa tgccgactta tggatctttt gttattatta gcggttttta gccatccatt
    12721 tttttgtatg attcgttggg cgttttgatt ttacctgaac cgcttttccg ccttttttcg
    12781 gaatattcac ttcaatattg tatttttctg atttgaccaa atcatataac ttttcagctg
    12841 cttcgtctac tttttctttc catttcatgt ggatccctcc aattcttgta gttaacttaa
    12901 ctctatctta
//
LOCUS       DAHUWM010000080        12889 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHUWM010000080 DAHUWM010000000
VERSION     DAHUWM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.081"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            121..3648
                     /locus_tag="NK788_001510"
     CDS             121..3648
                     /locus_tag="NK788_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCH6769845.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3645..7367
                     /gene="addA"
                     /locus_tag="NK788_001511"
     CDS             3645..7367
                     /gene="addA"
                     /locus_tag="NK788_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCH6769846.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7436..7777
                     /locus_tag="NK788_001512"
     CDS             7436..7777
                     /locus_tag="NK788_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6769847.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8086..9129
                     /gene="pheS"
                     /locus_tag="NK788_001513"
     CDS             8086..9129
                     /gene="pheS"
                     /locus_tag="NK788_001513"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCH6769848.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9134..11554
                     /locus_tag="NK788_001514"
     CDS             9134..11554
                     /locus_tag="NK788_001514"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCH6769849.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11724..12734)
                     /gene="trpS"
                     /locus_tag="NK788_001515"
     CDS             complement(11724..12734)
                     /gene="trpS"
                     /locus_tag="NK788_001515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCH6769850.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4378 a         2240 c         2736 g         3535 t
ORIGIN      
        1 cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat atagaacaaa
       61 tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag gaggaaaagc
      121 atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt cttgatcgac
      181 gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct tgttcccaac
      241 tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag aagcggaaaa
      301 aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc ttggtatttg
      361 ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc tgcaatgatc
      421 ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg agaaatcaat
      481 aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac aggaaatatt
      541 caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga ccagcaactt
      601 aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac tcaacgtgca
      661 ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca agatttcaac
      721 aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta tcaattactg
      781 caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg tccttataca
      841 gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta tttccgtctt
      901 tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc accagagcag
      961 actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc tagccaaaag
     1021 aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga agctgaaaat
     1081 ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag tgaagaaaac
     1141 taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg aaatatcctt
     1201 cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca aatgatggaa
     1261 aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta ttcttatcgg
     1321 ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga agcagaaacc
     1381 gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga catcactgaa
     1441 aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa agactggcag
     1501 tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt ggaagaacag
     1561 tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa aaaaataaag
     1621 aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat ccaaagtggc
     1681 gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa cttagaagcg
     1741 gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga atatgtagta
     1801 atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac aagtggtcta
     1861 gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat caatcgtttg
     1921 gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa tgatcaagtg
     1981 tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga agtattaaat
     2041 caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat ctcttttgag
     2101 cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct tagttatgca
     2161 caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag aatcacagaa
     2221 tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga ccctaattgt
     2281 tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg attagcttta
     2341 gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt actgaattcg
     2401 tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa tgttcctgtt
     2461 tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac ttcaatttca
     2521 cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg tttgaaattg
     2581 aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta tcatgaatca
     2641 ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa tatgtccgat
     2701 gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga acttcggttt
     2761 gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg acagacgatt
     2821 caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca tcctcttcag
     2881 acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct ggaattaccg
     2941 ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga tgtagcttct
     3001 gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg ttcattcgat
     3061 gtcacagaag cttattatgg catggctatg caattattga cgtatctgga cgtagcattg
     3121 atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc atattattta
     3181 catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac attgaaaaaa
     3241 tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact agatcattca
     3301 ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa agatgaatac
     3361 caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca actattcatg
     3421 gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg agaaatcaaa
     3481 ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc attcagaagc
     3541 atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga gaaaatagat
     3601 aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat caaattcctt
     3661 taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt gatcaaggag
     3721 ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt gttcgaagag
     3781 tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg acatttactg
     3841 aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa tccgtcaata
     3901 gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta ttgcccacag
     3961 cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat tattacttaa
     4021 ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc ttgatgaagg
     4081 aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa cgatttttcc
     4141 aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat ctggtgtttt
     4201 ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa cagttaagcg
     4261 ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa caaatccgtc
     4321 ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg actcagttag
     4381 cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa cagcagatca
     4441 agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct ggtttggaac
     4501 aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa atttccaatg
     4561 aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt aaaagttatt
     4621 ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg ttagtcgaag
     4681 aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa cgggaaaaag
     4741 gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca gaaaaaacga
     4801 aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc aaagaagtaa
     4861 tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac tggctaagag
     4921 aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa tccatctatt
     4981 ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc tcaaagaaag
     5041 aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa gtcctttctt
     5101 ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc aattatgacg
     5161 aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag tttgagccgg
     5221 aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg acggatgata
     5281 tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt cgtcaattaa
     5341 ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg atagagtaca
     5401 aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta gagattttca
     5461 aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa gcgacagaaa
     5521 ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat attccgcttg
     5581 cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc agcattcgat
     5641 tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca tcaaacgaag
     5701 atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg cgagaacttg
     5761 ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg ggatatctag
     5821 agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat gcattggttg
     5881 atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc gtgcgtttca
     5941 ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca atagaagata
     6001 atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca gttgtatttt
     6061 tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat gcttttgaag
     6121 aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta tatgatacgt
     6181 taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa gaaatgcgta
     6241 aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt tcctataaaa
     6301 ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag ttagtttttg
     6361 atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt tacggattga
     6421 tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct cttcttcaac
     6481 attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt gaacaaagac
     6541 agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa gatgaaactt
     6601 tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa gcttcttcac
     6661 aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat ccagatgata
     6721 cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc cgtcaatttc
     6781 gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg caagtgtcag
     6841 cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta gagatgccaa
     6901 ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta gtagacgaaa
     6961 aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca gaacttggtc
     7021 aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc atgctgcttc
     7081 ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta atccacggaa
     7141 tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt aaaacagatt
     7201 ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa tatcaaggac
     7261 agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta gaaaatgttt
     7321 tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta taattgagca
     7381 aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga gtatgatgga
     7441 gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct ctattttagg
     7501 aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc aacgctttgt
     7561 tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac gcttgaagga
     7621 attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga gccgttcaga
     7681 atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa tacaagtttg
     7741 gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa atcttgtaaa
     7801 ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg gctaaaccag
     7861 ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg aagttcactt
     7921 tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa gagtgcggtg
     7981 taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt aacaggaatg
     8041 aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc tctacaagca
     8101 caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca agatttaaat
     8161 gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac agaagtatta
     8221 cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt cgcaaatgag
     8281 atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga agaagaagcg
     8341 ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag aaaagtaaaa
     8401 catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat ttttgtgggt
     8461 atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa tttcgaacgg
     8521 atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta tatttcagac
     8581 gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga aaaacatgat
     8641 ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag agatacagat
     8701 gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga taaaaacatc
     8761 acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt tggggaagat
     8821 cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt agaagtagat
     8881 gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac tggatggatc
     8941 gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg aatcgatcca
     9001 gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat gttgcggtat
     9061 ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag tcagttcaag
     9121 ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag atttatcgaa
     9181 tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag tagagggagt
     9241 aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg aatgcgtacc
     9301 acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag acgaactgtc
     9361 tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg ttgcacttcc
     9421 taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg gccaagtatc
     9481 aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta taccgaaaga
     9541 atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc cagtttttcc
     9601 ttatttggat atggatgatt cgattattga attatctatc acaccgaatc gtgcggatgc
     9661 attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga ctcctacatt
     9721 cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc aagtggaagt
     9781 tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag taagaatcca
     9841 gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc caatcaacaa
     9901 tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac atgcatttga
     9961 ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa atgaaacatt
    10021 agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca ttactaacgg
    10081 caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga tcacgaatga
    10141 aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc gtcgtacagc
    10201 aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa tcaaccatgc
    10261 gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg cgggtgggga
    10321 agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat cagtgaagat
    10381 ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag aagtaaatga
    10441 gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt ccgtagtagt
    10501 accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag ttgctcgtat
    10561 ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg ctggaagctt
    10621 gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa gcggattgag
    10681 cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg ttataaatga
    10741 aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat tacgaatgaa
    10801 tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa atacagagat
    10861 tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta cacatctgcc
    10921 gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa aagattggca
    10981 gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa acttatttga
    11041 acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga aggaaatgca
    11101 tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg tcggtcaagt
    11161 gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg agtttgattt
    11221 atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga caaaattccc
    11281 agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc aggaattaac
    11341 agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat ttgatgtcta
    11401 tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga cattcgtcaa
    11461 cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag tcgaaaaagc
    11521 gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta agtaacgatc
    11581 aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc gtcacatctc
    11641 tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag gcctttacag
    11701 cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc tgattttact
    11761 tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg taagatttcc
    11821 ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg tgcaaattca
    11881 gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc gccgcggcgg
    11941 taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc caagtaggtg
    12001 aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt tggatcagta
    12061 tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata aataccgttt
    12121 cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc cattcctttt
    12181 ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt gaagctttgt
    12241 acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg tacaagattc
    12301 gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa tcctgtaggc
    12361 acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt gcgccgaacg
    12421 cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc cggaatttgt
    12481 gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata gtctaaagca
    12541 acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt cagtgcttgc
    12601 atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc cactcgggtt
    12661 tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc tcccgttaaa
    12721 ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac ttaatatctt
    12781 accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa gaggataaaa
    12841 taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgtt
//
LOCUS       DAHUWM010000081        12755 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHUWM010000081 DAHUWM010000000
VERSION     DAHUWM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.082"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            209..1840
                     /locus_tag="NK788_001516"
     CDS             209..1840
                     /locus_tag="NK788_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCH6769851.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1843..2922
                     /gene="uxuA"
                     /locus_tag="NK788_001517"
     CDS             1843..2922
                     /gene="uxuA"
                     /locus_tag="NK788_001517"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCH6769852.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2996..4423
                     /gene="uxaC"
                     /locus_tag="NK788_001518"
     CDS             2996..4423
                     /gene="uxaC"
                     /locus_tag="NK788_001518"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCH6769853.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4507..5154
                     /locus_tag="NK788_001519"
     CDS             4507..5154
                     /locus_tag="NK788_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCH6769854.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5283..6659
                     /locus_tag="NK788_001520"
     CDS             5283..6659
                     /locus_tag="NK788_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6769855.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            6670..7464
                     /locus_tag="NK788_001521"
     CDS             6670..7464
                     /locus_tag="NK788_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCH6769856.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            7477..9243
                     /locus_tag="NK788_001522"
     CDS             7477..9243
                     /locus_tag="NK788_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCH6769857.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            9271..11079
                     /gene="uidA"
                     /locus_tag="NK788_001523"
     CDS             9271..11079
                     /gene="uidA"
                     /locus_tag="NK788_001523"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCH6769858.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            11251..11652
                     /locus_tag="NK788_001524"
     CDS             11251..11652
                     /locus_tag="NK788_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6769859.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            11646..11891
                     /locus_tag="NK788_001525"
     CDS             11646..11891
                     /locus_tag="NK788_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769860.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(12215..12436)
                     /locus_tag="NK788_001526"
     CDS             complement(12215..12436)
                     /locus_tag="NK788_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769861.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(12598..12670)
                     /locus_tag="NK788_001527"
     tRNA            complement(12598..12670)
                     /locus_tag="NK788_001527"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12635..12637),aa:Lys,seq:ctt)
BASE COUNT         4525 a         1679 c         2537 g         4014 t
ORIGIN      
        1 tactcaaata agatcaaaga gaaattaagc tttaatttaa gttagttcta aaaaatatat
       61 attggtgtgc ttttactaaa aaaagacaca ataattctga catggtagaa aaaagaatat
      121 ttttagtttt ttcttattga cagcgattac attcgatttt atactacaat tgtaacggaa
      181 actaacatat cagtatggag gggtaaatat ggttttgatt aaagactggc aagattctat
      241 cgaaggattg aacaaaatgc aagttcaact accgtatttt aatgtcgaag cggtcaaaca
      301 agaaggtcag aaacatccag tgtggcttca ttttggtgga ggaaatcttt atcgaggatt
      361 tcatgcagaa attgctcaat gcttagcaga tcaaaatgaa ttagaatccg gagtagttgt
      421 gtgtgagaca tttgatgaag aagtagtaga aaaagcttat catgactaca acaatgatat
      481 tttggagatt gttatgggtg aagatggtga actcaaaaaa agacttttag cagtaacagc
      541 tgatagttta tattgcaatc caaagaatat aacggactat caaagagtgg ttaaatattt
      601 tgaagatcct acattacagt ttacaacgtt tactattact gaaaaaggat acagtttaaa
      661 agattctaaa ggacaatttt tttcggtagt ggaagcagat ataaaatctg gacctaccaa
      721 tcctcaacat acgatgagta ttgtgacatc cttgttgtat agcaggttta atgctggaga
      781 gttaccgatt gcgatggttt ctactgataa tttctcacag aatggtcaac gttttcaaga
      841 tagcattttt atgattgcag aagcttggca aaaaaaagga tacgtttctg aaaaatttat
      901 tgcttattta aaggataaag aaaaagtgac ttttccttgg agtatgattg atagaattac
      961 gccaaatcct tcggaaagtg tcaaaatgga tttattgaag gcgggtttcg aagacttaga
     1021 tataattcat actgcgaaaa ataccaatat cgctgctttt accaatactg aaaaagtcca
     1081 ttatctagtc attgaagata gttttccaaa tggtcgacct aaactggaag cggctggtgt
     1141 gattatgaca gatagagaaa cagttgataa agcagatatc atgaaggtta cgacatgttt
     1201 aaatccgctc catacggcat tagcaataac aggttgtttg ttggggtata aatcaattca
     1261 ggcagaaatg caagatgatg atttggttgg cttaattaaa gggataggtt atgtagaagg
     1321 cttaccagta gttgaaagtc caggcgttat tgatccaaaa caatttattg atgaagtaat
     1381 caataaacgt ttgcctaatc cctatattcc agatacaccg caacgaattg caacagatac
     1441 ttctcaaaaa gtagcgattc gctatggtga aacaattaaa aagtatgtcg aaaaagacgg
     1501 aaatgcagaa aatctaacat ttattcctat cgctattgct gcttggttac gttatttatt
     1561 ggcagtcgat gatcaaggaa aaaattttgt gccaagtcct gatcctttat tggtagagtt
     1621 gcaggaaaaa ttctcagtat tgaaattagg tgaacaagat cctgcaaaga ttcattgtgc
     1681 ggtacaggaa atcttagaaa ataaggaaat atttggttta gatttatatc aagttgagat
     1741 aggaaagaaa attgaaaaaa tcttttgcga aatgctagta ggtcctcaag cagttcgtaa
     1801 aacaatcaat aaatatataa cagaagttgg aggaaagtaa ctatggaaat gacttttaga
     1861 tggtatgggg caaatgaaga aaaaatctca ctacaagaaa ttcgtcagat cccagcaatg
     1921 aaaggaattg ttggaacatt aatggatata ccagccggag aggtctggcc gagagaacga
     1981 attaaagctt taaaagatga agtcgaagcc aatggcctta ccttgaaagt tattgagagt
     2041 gttaatgttc atgatgatat caaaattggt ttgccaacac gagatcaata catcgagaat
     2101 tataaacaga cgattcgcaa tttggcagaa tttgggattg aggttatctg ttataatttt
     2161 atgccagtct ttgattgggt aaaatcagat ttggattatc atttagatga tgggtcttct
     2221 acgttagcct atatttcaaa aaatattcct actgatccag aggaaattat tagaaaagta
     2281 gaaaattcta ctaataattt tgaattgccg ggttgggaac ctcatcggtt atcacaaatc
     2341 aagcaattat tagaagccta taaagaagtt gatgaagcga aattgcgaga gaactttgca
     2401 tattttctca aagcaattat cccgacatgt gaagaagttg ggattaaaat ggcagttcat
     2461 ccagatgatc caccatattc tattttcgga ttacctcgta ttgttaaaaa tcgtgacgat
     2521 ttagaatggt tgtgtaatgt agtggattct ccttctaatg gtattacgtt atgtactgga
     2581 agtattgcag aacgaccaga taatgatgtc tatgaaattc tatctgaatt ttgtcaacgg
     2641 gatcggattc cttttgcaca tgttcgtaat atcaaatatt taagtgataa tggactcgat
     2701 ttttatgaag cgccgcataa atcagaatat ggttctctag atatgtataa gatattaaaa
     2761 acaatgtacg ataatggatt taatggctat attcgtccag atcatggtcg gatgatttgg
     2821 ggagaaaccg gtagacctgg ttatgggtta tatgaccgag ctctaggtgc agtttattta
     2881 aatggtattt gggaagcgct tgaaaagggg gcgagtaaat aattatataa tggagtctat
     2941 ttagaagaaa aattttctct tctaaaaact aagggaagga aaggagtgct taaatatgtt
     3001 tttagataag aactttttat tagagaatga aatagctaaa agactttttc ataaatttgc
     3061 agaacaccaa ccaatcatcg attatcattg tcatttagac ccagcatgtt tgctagaaaa
     3121 taagcaatac aagaatttga ctcaggtatg gttaaatgat aaaggagctg gagatcatta
     3181 taaatggcga ttgatgcgag ctaatgggat agaagaagag ttgatcagtg gtgcaggtaa
     3241 tgattatgaa aaatttttag cttttgtaaa aacagtggag aaggcgccag gtaatcctat
     3301 ttttgaatgg tctcatttgg aattacaacg ttactttgat attgatttga caatttctga
     3361 gaagaatgca ccagaaattt ggcgacgtgc caacgaaaaa atccaaacgg attcctatcg
     3421 cccaaaagaa ttgctgaaga tgatggatgt gaaagccatt tgtacaacgg atgatcctac
     3481 agatgacttg cgatatcata ctgagctttc taaacaggat aattctttca aggtattacc
     3541 tactttccgt ccagataagg tctggagtat caaatcatca ggattttcga actatatttt
     3601 aaaacttagt caagcagcgg gtataccaat caaaaacttt aatgggttga agcaggcatt
     3661 gagccaacga gtggattatt ttcatcaaat tggctgtcga ttagcagatc acggtttgaa
     3721 tacttatttt tatgaaatag cagatgaaaa aatactcgac aatctgttaa taaaagcttt
     3781 agcaggaaaa acggatttcc atttatcaga aattgcacaa tttgttacag gtattcaatt
     3841 acatttgatg tctgagtata caagatatgg ttggacgatg caaatgcatt taaatgcttt
     3901 aagaaataat tcaagtatat taaaggaaca aattggagta gatgtcggtg gtgattcgat
     3961 gggggatcaa gctagtttga cacaaaatgt tgtagcattg ttcgacactg ctgaaaagag
     4021 tgggtgctta ccaaaaacaa ttttatattc attaaatcca accgattggc ttccactagc
     4081 aacagcaatg caaagcttcc aagggggtat gattcaaaaa atccaattgg gatgtgcttg
     4141 gtggttcaat gatacagctg aaggaatgcg tcaacagttg actacgatgg cgcaacaaag
     4201 tttgttgtcg aattttacag gtatgttgac tgattctcga agttttctaa gctatcctcg
     4261 ccatgagtat ttccgtcgag tattatgtca attactaagc gagtgggtta aagcaggtcg
     4321 aattccagat gatgaaatgt atcttagtca gatagtggag aatatttgtt atcgtaatgc
     4381 gtataattat tttaattttt ttgaaatcga aagtagaaaa taatacaaaa aataaaagct
     4441 taaatttatt tattgtaagt tagtacaact agctagagta tgtatccata aaagaaggag
     4501 atagatatga aaaaagtaga aattttaagt aagttagaag cggctggagt cattgcggtt
     4561 gtacgtggaa aaaataaaga tgaggccatt aaagcaataa atgcaattac tgcgggtgga
     4621 attaaaggga ttgaactaac ttttacagtt ccgaatgctg aagaaataat caaagaaatt
     4681 gctaaaaaat atgctgatca aaaagatgta ataattggag ccggtacagt tttagatgcg
     4741 tttactgctc gtattgcaat tatggctgga gcagaatata tcgtaagtcc atcttttgat
     4801 cgacaaacag cagagatttg taatttatat caaattcctt atttaccagg ttgtatgaca
     4861 attactgaga taaaggaggc acttaccgct ggagtagaaa tcattaaact atttccaggt
     4921 tctgcttttg gtccatctgt tgtaaaaacg tatcaagctc cattaccata tgtaaatata
     4981 atgcctactg gcggagtaag tctagataac ttagaagagt ggtttgaagc aggcgttgtt
     5041 gcagttggtg taggtgggaa tttgttggct cctgttgaaa aaggtgattt tgaagaagta
     5101 actcgaatag ctagtaaata tatagataag ttttatgaga taaagaatta ttaggagaaa
     5161 aaataaataa tactataaaa tgtaaaaaat aaatactaac tctttatact tcagagtata
     5221 gtacgtaagt tggcagttca acagaatgaa aacgcttaac aataggaagg agcgcaaatt
     5281 atatggaaag aaaatttggt ctaagagaca aaataggata tatgtttgga gattttggga
     5341 atgatttcac tttcattttt gctagttctt ttttaatgat tttttataca aaagtattgg
     5401 ggataagcgg agctaccgta ggaacattat ttttagtagc aagatttatt gatgcagtta
     5461 cagacataac aatggggcgt attgtggatg gatcaaaacc atctaaaact ggcaaattta
     5521 aaccgtggat tttacggatg agtggtccag tcgctttagc aagtttttta atgtatcaaa
     5581 catcaatggc atcagcttct atgagtatta aagttatata tatgtatgta acatatatac
     5641 tatggggaag tattttttat acatctatta atattcctta cggttcaatg gcatcagcta
     5701 tcacttctgt tgcagaagaa aggacagcgt tatcaacatt tcgtagtata ggagctacct
     5761 tagcaggtat gttcattggc gtgttaacac caatttttat ttatacaaga gatagttctg
     5821 gagctcaaat tgtacgtggt ggatcaactt ttacaattgt ggcaggagta ttttcagtgt
     5881 gtgcagttat ttgttacgta atatgttata aattaactgt agaacgggtt aaaagtgatg
     5941 aatcgcagtt aaaagtttca ttgttgaaat ctttaaaagg attagctaaa aatagagcgt
     6001 tgctttctat aatagctgca gctatttttt tgttgcttgc tcagctttta acaatgagta
     6061 tgaacaatta tgtatttcca gattattata ataatgcaca gggaatagtt ttaatgaact
     6121 ttatcaatcc tattttgatg ttggtacttg tttcacctgt aagtttaatt ttcagtaaaa
     6181 aatacggtaa aaaagaattg gcgtcagtag ggatgttctt ctctgctatt gtatatttac
     6241 ttttattttt gataaagcct agtaatatgt atgtattctt agttcttact tctgttggat
     6301 atatggggtt aggtattttt aatacagtca tttgggcgaa tattactgat gtaatcgatg
     6361 atcaagaagt gaaatctggc caaagagaag atggaactat ttatgcagta tattcttttg
     6421 cccgaaaaat tggacaagct ttagctggcg gtgtcggagg ttggactctt tctataatag
     6481 ggtatgattc gttagcaaaa gtacaaagtg gagaagtttt acaaaagctg tataactctt
     6541 caacgttgat tccagcagtt tgtttctttg ttgtaggatt gattttattt ttcgtatatc
     6601 cattatctaa agaaaaagta ttagaaaatt ataatttgct aaatcaaaaa cataactagg
     6661 agaaaaaaca tgaacagttg gatattttgt attgattcag atggttgtgt catggataca
     6721 atgacttata aacacaatct ctttttcggg ccaattgctg cagagatttt tgatgttcat
     6781 catagggaaa aattcctaca ggattggaat aatattaatt tatattcaga gactcgtgga
     6841 ataaacagat ttttaggttt agttaaaaca ttagaaaaaa atgatgtacc atcgattaat
     6901 cgattaaagc actgggtatc tgtaacaaaa gaattgtcta atccatcttt gaagagggaa
     6961 atagaatact ctcagtcaga ggatttatta aaagctttaa aatggtcgga agaagtcaat
     7021 cgacagatta gtcaagctaa aggattagat aaaccctttt taagtgctag agatactata
     7081 aaagctctga aaaaatatgg gaagataatt attgtaagct cagctaataa ggaagctatt
     7141 caagaagaat gggaaagaca tgaattatta tccttagttg atgaactgtg ttgtcaagat
     7201 aaaggaaaaa aagaagacat tattcgaagt gtaattgaaa atggttgtga tttggataag
     7261 atattaatga ttggtgattc tcctggtgat ctagaagctg caaataagaa taaagttttc
     7321 ttttatccta ttttggttaa taaagaaaaa gaatcatggg aaaacttaag aactgatatt
     7381 ttatttaaat gtttatcggg agagtatata gatattgaac aaaaatatat agatactttt
     7441 tggaaaaatc ttactaataa gtagaggaga aagaaaatga ctataaaagt agacttacga
     7501 aaaaaaccat atcatttaga tgaagatgct atttcttgga ttgaagagac gattgatgct
     7561 atgactttag aagaaaaaat aggtcaatta tttgttaata tgggatctag tcgtgataaa
     7621 aattacttaa gtagtatggt caatcagtac catattggag ctgtacgata taatccagga
     7681 actgctgatg aggtatatga tcaaaactat atcctacaaa cagaaagcaa aattccttta
     7741 ttaattgctg ctaatacaga agcaggtggt aacggagctt gtacagatgg taccgaagtt
     7801 ggtgtagaag taaaaattgg tgcaacaaat gatgcgaaat atgcttatga attagggaga
     7861 gtttctggaa ttgaagcatc ggctattgga tgtaactgga gttttgctcc tattgtagat
     7921 attaatagaa attggagaaa tcctatcatt tctagtcgat cttttggaca aagccctgat
     7981 aaagttttag aacttagttt agcttatatg aaaggtattc aagagagtgg aattttgcct
     8041 gcagctaaac atttccctgg tgacggtatt gacgaacgag atcaacatct atcttttagt
     8101 attaatagtt taagtacaga agaatgggat aattcttttg gaaaagtata ctcaggctta
     8161 atagaagctg gattaccttc aattatggca ggtcatatcc atatgccagc ttattcaaaa
     8221 cattttaatc ttgatctgaa agatgaagat ttacttcctg caactttatc aaaagaatta
     8281 atcactgact tacttagagg aaaactcggg tttaatggat tagtagttac tgatgctagt
     8341 catatgttag gaatgactgg ggcaatgaaa cggtcagaat tattgcccac ttctattgcg
     8401 gctggatgtg atctattcct attttttaat gatccagatg aagactttaa ttatatgatg
     8461 gaaggttatt acaaaggaat tatttcagaa gagcgtttac gtgatgcatt aagaagaatt
     8521 ttaggcttaa aggctaaact aggtttgcat aaaaaagaaa agaataaaat tttgttaccg
     8581 aaacaagaag ctatggaaaa aattggttta attgaaaata aatcaatttt taaagaggta
     8641 gctgatcaag caattacact agttaaagat aaacaagata tttggcctat tgtaccagaa
     8701 aaatataaaa ggattttact ggtagatgta catggggtaa aaggtgggtt tggtgaagta
     8761 gttgctggaa atagacctca ggccattgat ttattgaaac agattttgtc agcaaaagga
     8821 tttgaagttg aaaaatacat ttcacctgaa gaagaattga aaggattatc gcaagaagaa
     8881 aaaattaaaa atatagctgg aatatatgct gctaagagac caatcaaaga tttaactgaa
     8941 aaatatgact tgattattaa tatagctaat gttaacccag gtacagttca aagaattgtt
     9001 tggccagcaa gtaaaggtac tccggatatt ccattttata ttcatgaaat tccaacaatt
     9061 tttgtatctg tacagtgtcc gtttcattta gctgatgtgc ctcaagtaaa aacttatatc
     9121 aatacttatg atggaaaaca agaaacttta gaaatattag tggacaaact aatgggcctt
     9181 tctgaattta agggaataag tccaatagat gcttattgtg ggttaaagga tacatttatt
     9241 taatatttaa attatatagg agtgatttac atgttatatc ccattatgac agagacgagg
     9301 caagtaattg atttaagcgg tctttggaga tttaagttag atacaaatga tgacttacaa
     9361 gaagaacttg ctttagaacc aatggttgaa aaagagagtt atccaatttc agttccttct
     9421 tcttataatg atttatttga aacagagaac attagaaatc atgttggatg ggtgtggtat
     9481 gaaaaagaag tgattcttcc taaaaaatta ttagaagaaa gattagttct tcgttttgga
     9541 tctgcaacac atgaagcaaa agtatttttg aatggaaaat tgattaatca acacaaagga
     9601 gggtttacgc catttgaatc agaaattaat cagtttataa aagatggaag taacagaatt
     9661 acagtggctg taaacaatgt tattgacgat agcactttac ctgtagggat tttgaaagaa
     9721 acgatacagg aagatggaga cataaaaaaa gaaaatttgg ttaattttga ctttttcaat
     9781 tatgcaggca ttaatagacc cgttaaattg tatacaactc cgaaagacta tataaaggat
     9841 gtcactatta atacaaaaat aattgattca gatgcaaatg tagaatttaa tatcgataca
     9901 gaatggaccg gagaaattct aatagcaatc tatgacgaag aagctcaact tatagcagaa
     9961 gctaagggga aagaaggagt gattcaactt aagaacccaa cactttggga gcctctaaat
    10021 gcttacttat atagtatgaa agttagttta attaacgaaa ataaagtgat tgatacgtac
    10081 acagaagaat ttggcattcg tagtattcag gtttcgaatg ggaagttttt gattaataat
    10141 aaaccttttt actttaaggg atttgggaag catgaggaca cgtatattaa tggtcgtggt
    10201 ttgaatgaag cagtaaatat caaagatttt aatttaatga aatggatcgg tgctaattca
    10261 tttaggactt cacattatcc ttattcagaa gaaattatgc gcttagcaga tcgagaaggt
    10321 attgtagtta ttgatgaagt acctgcagta ggattacatt tgaatttcat ggctacttta
    10381 ttagatgaca acgtagaaaa acatgatact tggaaagaaa tacggacaga aaatgctcac
    10441 aaaatggcga ttaaggaatt gatagaacgc gacaagaacc atccgagtgt cgtaatgtgg
    10501 tctattgcaa atgaaccaga ttcggattct gaaggcgcta aagaatattt tgaaccttta
    10561 gttgaattga ctaaagaact tgaccctcag aagagacctg taacaatcgt tacttactta
    10621 aagtcaacac cagatatttg taaagtagga gatattgtcg atgttttatg tttaaatcgc
    10681 tattatggtt ggtatgtttc aggtggagac ctaaaacatg caaaatatct gttgaaaaaa
    10741 gagctagatg gatggatgga aagatgtccc gatacgccaa taatgtttac agaatatgga
    10801 gcagatacag tagctggact tcatgataca gtaccaacaa tgtttacaga agagtatcaa
    10861 gtgggttatt acgaagcaaa tcacgaagta atagataaat gtgacaattt tgttggtgaa
    10921 caagtgtgga attttgcaga ttttgaaaca agtcaaggga ttttaagagt gcaaggaaat
    10981 aagaaaggaa tctttactag agatagaaag cctaaaatga ttgctcacta tctaaagaaa
    11041 cgatggtcat caataccaaa tttcagttat aaaaaataat ttacctttta taaggaatat
    11101 aaaaaaggag tggctgctta attgccaact cctttttttt ataaaaagat tttctgttta
    11161 aagaaggata tttttttcta gttttttcga aaaaaaggcg ctttctgatt gttgtattag
    11221 tggaaagggg aaaaaaggag catatttatc atgcaacaaa attacaatga gaaatctata
    11281 caacatcaat ttgatgccta ctgtaaaaaa gtattacgaa acgaaactaa gtctattcga
    11341 aaacgaaaca ttaacataac agataatgag gaaccattga cgaattcaaa tgaaggtaag
    11401 tactcccaaa ctcattttga tgaacaagat gtctatttct tatttggaat ggaaatatta
    11461 atttcagatc aaaagttatc ggcagccatt gatcatttat cggaaataag aagaaaggtt
    11521 atcctacttt attattttgc aggttttaat gatactgaaa tcggtaaaat cctcaatatg
    11581 agtacaagtg gtatctggta tcaacgaaag aaggcagttg aacaattaaa aatggggtat
    11641 gacttatggt agacaacccc aaaaaacggc agtatccatt agttcctcta tccattatcc
    11701 aatctgctct agaagaagat ccaatttcaa tcaagatgat actcaaacat tatcatagtt
    11761 atatagctaa gttatgtttg acaaatggat ttaatgaggc ggggcaattt attacctatg
    11821 tagatgagta tatgctaaga cagctagaaa ttaaattgat agaagcgatc ttaaaattta
    11881 aaattaacta actaaaaagt cccctttcca cttattctgt ttaaaagaat cttctaatag
    11941 ttaaaggatt agtagattct tttaaacaga aaacctattg atcattgaaa tttaaataca
    12001 ttctatcagt acgttaacac ttcaaggagc atattttagt tgcgccatga tttcttactg
    12061 taaagaagga gcgataaaca ttctaaaata atttttatag tcaataaaaa gcgatgattt
    12121 gaagttggga taatgatact tccgtaaggc tcgccgtaaa cttggagaga ggtgaaattc
    12181 ctatgtgctt ttgacaaaag cagctgatta agaattattt ccatatgttg atttcaacat
    12241 tttttctatt atttttaaga ccaaattcag tgaggcaaat agcatcatgt aaatttagtt
    12301 tttctattga acgttctcca ctttttagtt tttttatagt actcaaattt aatcctgtat
    12361 gtttagagat ctcatttgca gtatggtttt gtaaagtttc tcggattatt gttatattag
    12421 caatttcatt acgcataatt atgttccttt cttatcaaaa atatcaatga ctacagtggg
    12481 cttttagtga aaagaagggg tactacagtc tcttttgaaa acaaaaaaat acagtatata
    12541 cgttttctaa atgctaagca ccaacgttta cactgtattt aagggtttgg cacccaatgg
    12601 gctgtgaggg gctcgaaccc gcgacccgat gattaagagt catctgctct accaactgag
    12661 ctaacagccc aaattaatac tttgttattc taacacctaa aataaaaaag tgcaatactt
    12721 attatcagaa ttgagagtga attttatgga ttttc
//
LOCUS       DAHUWM010000082        12732 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHUWM010000082 DAHUWM010000000
VERSION     DAHUWM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.083"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NK788_001528"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NK788_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCH6769862.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="NK788_001529"
     CDS             complement(322..891)
                     /locus_tag="NK788_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCH6769863.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="NK788_001530"
     CDS             1077..1979
                     /locus_tag="NK788_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6769864.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NK788_001531"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NK788_001531"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCH6769865.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NK788_001532"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NK788_001532"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCH6769866.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NK788_001533"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NK788_001533"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCH6769867.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="NK788_001534"
     CDS             8547..8732
                     /locus_tag="NK788_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCH6769868.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="NK788_001535"
     CDS             complement(8794..9990)
                     /locus_tag="NK788_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCH6769869.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="NK788_001536"
     CDS             complement(10160..11596)
                     /locus_tag="NK788_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCH6769870.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="NK788_001537"
     CDS             11889..12698
                     /locus_tag="NK788_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6769871.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2674 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DAHUWM010000083        12505 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHUWM010000083 DAHUWM010000000
VERSION     DAHUWM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.084"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(114..476)
                     /locus_tag="NK788_001538"
     CDS             complement(114..476)
                     /locus_tag="NK788_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769872.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(550..930)
                     /locus_tag="NK788_001539"
     CDS             complement(550..930)
                     /locus_tag="NK788_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCH6769873.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            994..2187
                     /locus_tag="NK788_001540"
     CDS             994..2187
                     /locus_tag="NK788_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6769874.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2264..2848)
                     /locus_tag="NK788_001541"
     CDS             complement(2264..2848)
                     /locus_tag="NK788_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6769875.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3049..4047
                     /locus_tag="NK788_001542"
     CDS             3049..4047
                     /locus_tag="NK788_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6769876.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4159..4668
                     /locus_tag="NK788_001543"
     CDS             4159..4668
                     /locus_tag="NK788_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCH6769877.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4698..5357)
                     /locus_tag="NK788_001544"
     CDS             complement(4698..5357)
                     /locus_tag="NK788_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCH6769878.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5359..6123)
                     /locus_tag="NK788_001545"
     CDS             complement(5359..6123)
                     /locus_tag="NK788_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCH6769879.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6131..6808)
                     /locus_tag="NK788_001546"
     CDS             complement(6131..6808)
                     /locus_tag="NK788_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCH6769880.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6845..8230)
                     /locus_tag="NK788_001547"
     CDS             complement(6845..8230)
                     /locus_tag="NK788_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCH6769881.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8440..8524)
                     /gene="ffs"
                     /locus_tag="NK788_001548"
     ncRNA           complement(8440..8524)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NK788_001548"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8547..9062)
                     /gene="tadA"
                     /locus_tag="NK788_001549"
     CDS             complement(8547..9062)
                     /gene="tadA"
                     /locus_tag="NK788_001549"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCH6769882.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9169..10026
                     /locus_tag="NK788_001550"
     CDS             9169..10026
                     /locus_tag="NK788_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6769883.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10107..10442)
                     /locus_tag="NK788_001551"
     CDS             complement(10107..10442)
                     /locus_tag="NK788_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769884.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10509..11879)
                     /locus_tag="NK788_001552"
     CDS             complement(10509..11879)
                     /locus_tag="NK788_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCH6769885.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11957..12493)
                     /locus_tag="NK788_001553"
     CDS             complement(11957..12493)
                     /locus_tag="NK788_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6769886.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3709 a         2548 c         2102 g         4146 t
ORIGIN      
        1 aaatgcacta aaaaactatc tttagcagaa catttcattg tattttttgg accaaacaat
       61 aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt attttagaag
      121 aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc tggttcttcc
      181 caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc aaagacaaac
      241 aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact aatcttgcta
      301 acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc agtctttcct
      361 tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac caaaaatttt
      421 ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct tgccaaaatt
      481 tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt ttcagaaaaa
      541 atacactttt tatttcttat tttctttccg ttctagttca gataatgctt cttctatcca
      601 aataggtaac tgctccgcaa tagtggtaaa tccgatctta cgatttttat tcgtgaaata
      661 cctttttctg cgatatggaa cagctggatt tgttttttct aacgttctta aagataaact
      721 gatttttttg gaatattcat caatgtcgat gatctgaact gttactggct gtccgacttt
      781 taacaatgaa tggatatttt tcgtataacc ggattgtact tctgaaacat gaataagtcc
      841 ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc tgtatccctg tcacttttcc
      901 ttcaattatt tgtccaattt tataagtcat ctacttccct ctcttcgcat ttatcattat
      961 actgtaaata gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa
     1021 tttgataaac agatttcaag aaaagataca gaaagtgtaa aatgggacgc aattgctgag
     1081 acatatcaaa tggatgactt acttcctttg tgggtagcag atatggactt cttgtctccc
     1141 ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg ttatgccact
     1201 ctgtctgata aactatatca ggctatcatt cattgggaac gccaacacca tgctgtggag
     1261 ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta
     1321 caaactttta caaaaccagg cgattctatt ttgattcatg acccagttta tcctccattt
     1381 tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat
     1441 aatcatttca tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa
     1501 gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa agaagaattg
     1561 aaacagttaa gcgaattgtg tttaaaacat caagtgattc tgtttagtga cgagatccat
     1621 caagatttaa ctttattcga tcatacattt acttccatgc taacgattga tcctaattta
     1681 gatgatctac tagttgcttt tacttccgca accaagactt tcaatctagc tgcaatcaaa
     1741 aattcaatgg tatttatcaa aaatccagag atgaaagctg attttgaaaa acatttagtg
     1801 atgaaccaac agcacgaaat caacactttt ggactgattg gaacccaagc ggcttatgag
     1861 acgggtggag aatggttaag ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt
     1921 aatcagtttt ttgaacagta tcttccgaaa gtgagaataa tgaaccctga aggaacttac
     1981 ttaatgtggt tagattttac cgcctatatt tcagacgacc aagaattgga gaacactctt
     2041 gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc cggtcatggg
     2101 catatgcgct tgaatattgc ttgtccagaa gaaactttac tggaaggact taatcgaatc
     2161 aagtatgctt tagagacaat cgattaaatc atttattcaa aataaaagga cgagacctta
     2221 ctaggtttcg tcctttttca taattactcc gtaccatacc aaactattct gaaatttcaa
     2281 ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg accacgttgt acatttgcga
     2341 tttcatctac tgtgtccatc ccttctagta catgtccaaa gactgtatgt cggaaatcta
     2401 gccaaggagt tccgccacct ttatacgctt caatgatttc tgatgggaat ccagcacctt
     2461 ctagttgtcc aagcatatta gctggcacat ttgtgttatt tacaataaag aattggcttc
     2521 cgtttgtgtt tggacctgaa tttgccatag ataaagctcc gcgaaggttg aacagttcac
     2581 gactgaattc atcttcaaaa ctttcgccat aaatactttc gccacccatt cctgttccag
     2641 ttggatcgcc gccttggatc atgaaatcag gaataacgcg gtgaaaaatc acgccatcat
     2701 aataaccttt tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct agttcaggga
     2761 ataattgaac agtaatgtca ccacggtttg ttttgataac agcttttggt cctttttcac
     2821 tttttaaatc tagttgtggg aatgccatat tgtttcctcc taatatttgc atacttcata
     2881 ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt cttttatttt
     2941 tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac atttttgaga
     3001 agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat ggaaatctat
     3061 gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta cgcaggaatg
     3121 cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca attagcaact
     3181 ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa agcaagtgaa
     3241 ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca tttaaaagaa
     3301 gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa taaaggcatc
     3361 cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc aagaaaattg
     3421 aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt gaatgatttg
     3481 atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc tatcgattgg
     3541 acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt
     3601 cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt gcttactccc
     3661 tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg gttaaaaaaa
     3721 gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa ttatggattc
     3781 tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa tgccattact
     3841 gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga catctgtacc
     3901 tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac tgcagtgaac
     3961 aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag cacaagctta
     4021 tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc aagttaaacc
     4081 cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa
     4141 taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa
     4201 ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat gtttttacaa
     4261 aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa cagcgtgcta
     4321 acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat attagcagaa
     4381 aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg cctttacggt
     4441 atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat
     4501 tatggttata tcgacaaggt gaaacctggt attttaaaag aactaaacag ccacgatggc
     4561 gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc tgctgccagt
     4621 cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa
     4681 agaaagaaaa cgtctattta gacgagttct ttcttttttg tttgtttcaa gctatttttt
     4741 cctaagaaaa gaaaaacagc aaaaaataat tctaataata caaaaaataa gataaagcta
     4801 tgcatgaaaa attcttctgg aagtacattg atgattggat cagtaacggg atcgaataac
     4861 cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc aaatcctatc
     4921 atcatgaaaa aaccgaaaac aactggcagt aacatgccga tttgaaacgg acgaatcaac
     4981 cgccaaagcc gatcattctt ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg
     5041 atgaaaaaca cagcataatc cactaaaaac aaaattttca cttcataaaa gtgatgggct
     5101 ccgctagcag aaactgggaa atctggcaag ctgaggatgg ttttgaaggg attattgaga
     5161 taattcatta ggtgatcaaa atttttcagt aacgtctctt gatctaaaga agtataatcc
     5221 aaaatttgca aatgtccgat atcaaatacg tatagtggtc gaaagttgat cgtcacacca
     5281 actgccagac tgatcagtgt cagaaatagg ctgaacaatc cagcatattc catccactgc
     5341 catctcgaat ttttcatatt agaagctcca ttcatccagt gattgtttca cataggtagc
     5401 tgggataggc aacccaggta catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt
     5461 atctatatta ttgcgtatcc cagcttggat atctgtttca tagttatctc caaccatgat
     5521 tacttcttct ttttgcagcc ctaatacttc aacggcttta tccataatga tggcttctgg
     5581 ttttccgatg tagatcggcg gcgtttgggt tgctgtctct acaaaagaga tgactgaccc
     5641 tgctccagga agaaggccgc gttcagtagg tatattctta tctgggttcg ttccaatgaa
     5701 agtagcacct ttttgaatag caagtgttgc tttaactact ttttcatagg tcagataatt
     5761 atctaagcct accactacat agtcaggagc ctcttcttcc catgtaaatc cggcagctaa
     5821 aatcaagtca atcaatcctg cttctccgat aacatagact tttttccctt ttccatctgc
     5881 tttcataaaa tcgattgttg ccaaagtagc tgtgtagact gtctctggag ctacatgaat
     5941 gtcaaactca tctgctagac gattagctac tgtttctggt gacttagtcg tgttattggt
     6001 aacaaacaag aaaggtaatt ttcttttttg caattcttcc acaaatcgtt taccggccgg
     6061 tatgggttgt gtcccacgat aaatcgttcc gtctaaatca attaaatatc ctttatagtc
     6121 cattcatcca ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt ttttctactt
     6181 gtttaggttg ttcttgtttt tttacttttt cagaggtttt tgctgactct gtttttcgat
     6241 ttttaataac cggttttttg cgattagatg cgactggtcc ttttttctca tcgatatacg
     6301 cacgattatg gttttttttc ttttttcggc gctgagttgt cttttctttt ttaccgccga
     6361 ttctctcgat gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca
     6421 aggaatcgat tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa aatcctttta
     6481 ggcgtagttg ttcataaccc caatctccta cgatataatc atatcgggct agtacatcac
     6541 tgtaacgttc tccaagcttt tctgcattaa atccttcacg ataatcggac acaagccgat
     6601 aatgtctttc acctatcaaa aaattactgt catctaacag tgtcactagc tcgcctttga
     6661 ctttttctgc tttaggctcc tcaacgattt cttccaaagc agctgtcagt tcttcggtga
     6721 ctgttgtttc ctgttcagct gatgattttt tcttagcaat ttctgtttta tgctcaacgt
     6781 tctgctgttc attttgtttt gcagacatgc gttcaccacc tatctttttt attatacctt
     6841 gtttttattt gattgggtag tgagctttca agtaatctcc taccacactt cggataaatt
     6901 cagggaacaa aaattcattt tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca
     6961 tcaaatgatc tacggtagta aaagtataat ttttattcag atcgattggc ttgttcttcc
     7021 aaaagacttg atggttaata gaatcatatg tgattccatt atagaccatc tgcccaaaaa
     7081 tcttaccgcg aaatcccatt cccttcatag gatagttacg taaaaagtta cgattttttt
     7141 ctatttctaa aactaagcgg atcagatcat ttccacccaa tgtgacattt aacaagtgca
     7201 tcggatgcgg caacgcagtg tgaagctgat cctgattgac ccgccctttt ggaatttcag
     7261 ttaaaaacag accactgttt aatattgctg cttccgtctt tcctcgttct ttcaccgcct
     7321 caaaggctac ttggataaac gaatgctcat tgaagatatc tagactcaga gaaaatggca
     7381 aatctgctac ttctttttct tttaataatt catgcccacg ttctagataa ctatttattt
     7441 cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg gatggctttg gctgaatgct
     7501 taaggatttt tttctcttca tcgatggtta aatggacttc accgacatac tgcccgtact
     7561 ttccagcggc tgctatttgg acatcattga ttacgtgacc ttctctgaat aaatgatggg
     7621 tgtgcgatcc taaaataaca tcaattgctg gtatttcttt tgcaatttgg aaatcatcct
     7681 gaatcccgag atgactcatc aaaactaaaa catccacttg tgaacgtaaa tcttcgacta
     7741 attctggcaa aatatcataa ggattccgta tgtcccagcc attcggatta taagtaagcg
     7801 gaaatggagc agtaaatgca attagtccga ttttcgtctg gtgtttcgtt gtaatgattt
     7861 tatatggttt cgtccatgcc ggtggctgga gcgtattttt atcgaataag ttatccagga
     7921 tgatatcaaa ctcagcatga tcgtataaat gattcaactc ttctttggaa tttcctacgc
     7981 cttcattgtt tcctatcgtc gctgcatcat aaccaacttc attcatcagc aagatatttt
     8041 cctgtccatt cgttgcttct gacaatggat gccaacgatc cacaaaatca cctagatcta
     8101 ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg tttacgttga tccaaaaacc
     8161 ttctgatcct tggccaattt tctaaatgag aatgcagatc attcgtatgt aaaatcacta
     8221 ttttttccat gtaccctctc gcctctcttt atctttatca taccataggc agctttttct
     8281 tccattgaaa caagctctat ttcagagttt ttcctgcctt gaagcaaatt gtataagtac
     8341 aatattcgat aaatcagtct ggctaaaaaa tcaggaacat tcatttttac agtactataa
     8401 aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat tataatcaga cacatggcgg
     8461 aacctgctta gtgctgcttc cttccagacc tgacacgctt cacaagccca ccattgtcct
     8521 agcctttcgg caaagaatag tataccttat ttttcattcg ttgaccagtc tttttttaat
     8581 ttcttttttc gctggcggag ctttcggaaa aagtcagtca aaatcatccc acattcgtct
     8641 tctaaaatgc caccttctac atatgcttgg tggttaaacc gctcgtcctc caaaagattc
     8701 atcaaggtac cggctgttcc gccttttgga tcatacgcgc cgaaataaac ttctgctaca
     8761 cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac aaaaagtgca
     8821 gcatcttcca agcgccagct tcccactttt tcacaggctt ccttgattgc aagcatttct
     8881 gcatgtgtag tagcatcttg cgtggtttct ctaaggttat atcctcttcc aatcactttt
     8941 ccatccaata caaccactgc tccaatcgga acttcatgaa gcatttctgc tttctttgct
     9001 tctgccaatg caaggcgcat ccatttttct ttttctactg catcaagatt tgtatttacc
     9061 aattaccctt ccctgccttt gttttttctt gttttacttt agcatatgat aaaaaggctg
     9121 taactaactt ttttttctat ggtaaaataa atacaagagg tgaacaagat gaaaatggat
     9181 aaattacttg ctctttaccc acaagcacag aaaaaaaact ctccagcatc agaagcggat
     9241 atcctttctt ttgcaattga tgatgctttt atctggatca aacaagattc attatctcaa
     9301 caagaaacca gccttttgaa agcgttattc cctgtaatca acgatcaaaa aaaacaccca
     9361 tggtatcgct atctttttaa agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc
     9421 cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga tactattttg
     9481 gaattatttc gctattctgc tgatttcttt ttttatacag acacagatgc gctcttagtc
     9541 gaacaaataa accgtgatta tcttgatgct actgaaatca acggtatttt tctatcttta
     9601 gatgctgatt ttgatacgac aacgtctgta ttcgttggtt ctttccattc acctggcacc
     9661 gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt taacagagca aaacaatctt
     9721 tatactcaca gccgtacttt ttctatgcaa gatgtggctt tacattacta tacaaaagat
     9781 actgtaaaag atagtaagtt gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg
     9841 gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca
     9901 ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt
     9961 ttcaatctaa aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat
    10021 aattaaagat agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat
    10081 gaatagtgtt caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg
    10141 tcttttacat cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt
    10201 cctgatttga tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca
    10261 tctacgattt ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact
    10321 ttcgtccgag tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct
    10381 gtcgctacac tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc
    10441 ataagtatca gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac
    10501 taatccactc agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact
    10561 tgtttcccaa atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca
    10621 tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag
    10681 caaggtatat tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga
    10741 ttctttttga aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa
    10801 ggggctaact caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct
    10861 tcataatgaa aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc
    10921 actttacttt tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc
    10981 agctttgcaa caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt
    11041 tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga
    11101 atctcctcaa tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct
    11161 tctggtgcaa acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt
    11221 gctgaagaca aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac
    11281 tcttttattg catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc
    11341 acgatccgat caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc
    11401 actattgcgt tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc
    11461 ggaaagatct cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt
    11521 gatttaccac cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca
    11581 gctgttccaa cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg
    11641 aaagactttc cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat
    11701 tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga
    11761 aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct
    11821 aaaagaagac gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt
    11881 ccaatgtttc gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac
    11941 tttttcatgt tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg
    12001 tcacctcgtt tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat
    12061 ctgagcgcaa acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga
    12121 tcttcgtgtg acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta
    12181 aatcagattt tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata
    12241 agtagacaat gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa
    12301 cagtgatttc ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa
    12361 tcgggtatgt agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc
    12421 catattcttc cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta
    12481 ctctccccat cattcttacc tccgt
//
LOCUS       DAHUWM010000084        12252 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHUWM010000084 DAHUWM010000000
VERSION     DAHUWM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.085"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..352
                     /locus_tag="NK788_001554"
     CDS             <1..352
                     /locus_tag="NK788_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769887.1"
                     /translation="LGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVAS
                     INGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTF
                     SKQKQPRREVQHSH"
     gene            complement(497..1237)
                     /gene="truA"
                     /locus_tag="NK788_001555"
     CDS             complement(497..1237)
                     /gene="truA"
                     /locus_tag="NK788_001555"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCH6769888.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1428..2063)
                     /locus_tag="NK788_001556"
     CDS             complement(1428..2063)
                     /locus_tag="NK788_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCH6769889.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(2210..3451)
                     /gene="sstT"
                     /locus_tag="NK788_001557"
     CDS             complement(2210..3451)
                     /gene="sstT"
                     /locus_tag="NK788_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCH6769890.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3649..4647
                     /locus_tag="NK788_001558"
     CDS             3649..4647
                     /locus_tag="NK788_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769891.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4660..6678
                     /locus_tag="NK788_001559"
     CDS             4660..6678
                     /locus_tag="NK788_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCH6769892.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6738..7454)
                     /locus_tag="NK788_001560"
     CDS             complement(6738..7454)
                     /locus_tag="NK788_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCH6769893.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7681..8223)
                     /locus_tag="NK788_001561"
     CDS             complement(7681..8223)
                     /locus_tag="NK788_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCH6769894.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8228..8455)
                     /locus_tag="NK788_001562"
     CDS             complement(8228..8455)
                     /locus_tag="NK788_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCH6769895.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8534..10414)
                     /locus_tag="NK788_001563"
     CDS             complement(8534..10414)
                     /locus_tag="NK788_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCH6769896.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10603..11217)
                     /locus_tag="NK788_001564"
     CDS             complement(10603..11217)
                     /locus_tag="NK788_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769897.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11359..11919
                     /locus_tag="NK788_001565"
     CDS             11359..11919
                     /locus_tag="NK788_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6769898.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3853 a         2429 c         1952 g         4018 t
ORIGIN      
        1 actaggtaac caggaaataa atccaacaac tcattttatg agtaacaaac ttacaattga
       61 attagataat tcgaataata aaaatactgt cttgacgaca cctatttatc gttataaagg
      121 gcaagtggct tctattaatg gcaaactagt acaaacaaaa ttatctaagt ttggcacaac
      181 tgaattaact attcctccag gtataaataa agtcgttatt acttatcagt acacaaaact
      241 agcaattgct tcaagatatt tatctatcgt tactttgatt ttatttttac tttatcgatt
      301 tactttttct aaacaaaaac aaccgagaag agaagtacaa cattctcact agatttttat
      361 ttaagaaaga ctttttagct gtacacataa acaggctaaa aagtcttttt ttatatttct
      421 ttaatttgct tcatacatta tttaacatcg atatttatac tgtgaaacat gcgtttcatc
      481 cattatcaaa gtatttttat cgataataaa cttcatccaa aaacagacct tgtgcaggga
      541 cggtttctcc tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt gtttctatcg
      601 gcatggcacc tgtgccgatt tctagtaatg tccccatgat aatccgcacc attttatata
      661 agaatccgtc tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg atagataatt
      721 catcaatcgt acgcactgtg gatttttttg attttttcaa tgcagaaaaa ccaatgaaat
      781 catgtgtacc cactaatttt tcgcaagctg cattcagctt gtccatatcc aactcttgag
      841 ggaaataaaa gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc caaacataat
      901 agctgtattt tttgcccacc gcattatatc ttgaatggaa gcgctcaggc atttcttcga
      961 ttcttttgat gacaatatcc cgcggaaggt aacgattcat aaagtcgatc atttctgcca
     1021 gtccaatttc tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg gtgccagcat
     1081 ctgttcttcc tgatccaata atttctatct cttcatttgt catctgatgc aatacttttt
     1141 cgatttttcc ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct gaatatcttt
     1201 ttccgtcata ttcgattgtc aacttgatgt ttctcatatt ttagtggctc ctctgtcttc
     1261 catttggtct tctctctacc tttttaagat tgtagcacaa aatatagctc cttgcttctc
     1321 taagacagaa gctatacaag aaaactgtgg aaatatatat ttcagaaaaa caaaaaacag
     1381 ccaccggatg aagtatccgc tgactgttca atttgacggc tcaagtttta ctgtttgaga
     1441 tgcaagatac tcaacctggc aatgggcaca taattaaaga aaaaactgat tctttggcaa
     1501 aaacctattt tattcagtaa tggccaagaa tttatagaag gaattcttgc cagtccataa
     1561 atcgccgcaa aaagcaaact gatcacagac agtccaaggt ataagtggct tacgtccatt
     1621 tgcccatttt ttcgatagat caagaaaaga agaactggaa acaataaaaa accactgtaa
     1681 cctaaacctg atccgatatt atggacccat gtcgagacat tccacgaaga ttcatgggta
     1741 ttgatactga acaaacctgt aaagatacaa tcacctatac catagaacgc gatggctgct
     1801 gtaactaggg tagctaacac acgagatgta ctaacaaatg cagcatacaa tgcaggcaat
     1861 gacaggatga aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt tcttacagga
     1921 ctggaaacct ctccaaatac actgatcacg ctcttcattt gatctaattc aggataatac
     1981 aatcctaata cgtacggagt caaaaaatca ctcagcacgc ctaataataa aaagaaaaaa
     2041 ccaaatttac gtaaaaaatc catgatacgc ctccaataac aattacctat gcttttaatt
     2101 gttattataa acgactttat cttgatttaa cagaaacctc tatttacacg aacatctata
     2161 taactcatta aaaactcgaa tcattttaaa aatactaaat atctaacgtt caacgttcac
     2221 ttaatccatc taaacaatct ttcaatttga ttttttcccc acttctttta cgcgcgccta
     2281 gctctcctat tgcagtaaac aacagatcac ttgacgaatt taaggcagtt tccattgaat
     2341 cctggataac accgataata aatccaatac cgacgatttg catcgcgaca tcattagaga
     2401 taccaaacaa actacatgct aatggaatca ataacagtga accgcccgca acacctgaag
     2461 ctccacaagc agctaaagca gacaaaatac ataaaaggac tttcaaaaca aaaggtgctt
     2521 caatccctaa agaatgtaca gcagtcaacg tcatcaagga aatggtaatc gcggcaccgc
     2581 ccatattgat cgtagcacct aacgggatcg atatggaata agactctttt gtcaatttca
     2641 atctttcagc caacatcatg tttatcggga tatttgcggc agaacttcgg gtaaagaaag
     2701 cgggaagtgc gctttctttc aaacaaaaga aaatcaatgg atagggattt tctttaataa
     2761 acaaaaacgt gaggaatggg tacacaacta atgcgacaaa cagcatcgta ccgatcaaaa
     2821 caagtaaaag ctgcagatac tctgccaatc cagcgattcc agtcgtagca attgattgat
     2881 aaaccaatcc taaaatccct atcggagcaa atgcaataat cacttgtacg acttgtgaca
     2941 acgctataga tatctgatcc accaactctt ttgtcgattc tgaacgtgct cgaaaagcta
     3001 gtcccaacaa tacagcccag aataagacgc caaggtaatt cgcatcaata agagattgaa
     3061 caggattttg tgtgacattc gtcaacatag tagataaaac actttccaaa ctttgcggtg
     3121 ctttttcaga tactgtttct tgtaaagtaa taggaacagt aaaaattgaa cttacaataa
     3181 ctgctacaag tgcagaaagc aatgttccca ctaaatataa gatcaaaata ggcttcacga
     3241 atgtcttatt cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa aaaaccagaa
     3301 taggtgcgat tgccttcaag caactgacaa acaacgttcc caatattgaa ataaatgtcc
     3361 atgtggggaa aaagatacca aacaatattc cgactaaaat accaacaaca atacgttgaa
     3421 ctaacgacat atccaaaaac ttctctaaca aatgaccacc ctttcacaat tttaacaatc
     3481 attgtatcat tttatgcaaa gaaactaaag gtataatttt ctaaaaattc taacaataca
     3541 aacaaccatt tcttgctaat tttgtacttt ttctgtctac acgttgtttt ttgagatatt
     3601 ctaagttagg atgttattaa tgggcaactt atcaggaaac gttttcttat gtttttgtcc
     3661 aatttttttg tgaggtgttt attcttgaaa aaaataaaga aggagagtta tggcttcctg
     3721 acaatctttg tcgcttgtat aatgatggtt ttacagcgta tattgatttc agcagtcact
     3781 attcctacat atgcaacaaa ccctggtcga ttctacatct atacattttt acaaacttcc
     3841 gttgttattg gtgcaaattt gatcccactt tacatgggtt accaatatca aaaaatcgcc
     3901 cgtttaaaag ttttacatta tttaagtcgt tttgccttta tattcctaat agccacacta
     3961 gtttgcaata tcttttttta cgtacaagct ggcagcttga atattcgtga ttattggctt
     4021 atcctaacac ctatcagtca gaattacttt acttatgctg tttcttgtac tttgcttttt
     4081 tgtagtattc cttatacagt tggattttta aataaattat cgaaaacaac aattaaatat
     4141 ctactattat ttttaacaat tggattagtt ggctgctcta ctctatttaa taaagacccg
     4201 tttgcattac agaatggaca gagtatttta tggatttttt atttattttt aatgggttat
     4261 ggtttgaaag aatggaactg gaaagaaaag gtccgctata agcttcctca agtatttatc
     4321 agcttgattg ttttattcgg tttgattatt ttgatgacac aaatttcact catcatcaga
     4381 ggaaatacag atacagcgct gaggttttct aaaccatatt ctctcttcgc aatgtattat
     4441 actttctctt cgtttttatt tttagaaatg ctatctgata aattaggtat aaaaggaaga
     4501 ggaaattttc ttgctgtatt tttgattgtc actcaagtta tatgtaattt ccctttagtt
     4561 tcttatcgcg tatctacatt tatgaaacgt gaatttccaa actcagggaa ggcatggctg
     4621 ataaatatcg ggcagttttt tatttgatat ttattagcag tgtccctcct agttatcttt
     4681 ttattttata tacaaaaaat ccgtttcatt catcgtttca tcgaatactt cgcttttgat
     4741 tcgattgaac aattaactca gcgttggaaa aaaagaattc attggctttt tgttcataaa
     4801 agttattttt tagttgcagc atttttttac tgctttacct tcgtgcagat tttcgtttta
     4861 gaaccaaaag aaggttggaa agaaactatc caggttgcct taaaaatatt tactcagcga
     4921 caagcaccca tgatcctgac aatcatcatt attatgggat ttttcttctt actattgttg
     4981 atttccaatc gtttttggta cgccttgact gctaccttga tcattaactt actgctcact
     5041 atttcgacag taatcaaaat ggaaatgcgg gaagagcctg tctttccttc agatttaaaa
     5101 atgctgaccg gattgtcaga actattatcg atggttagcc cagtcttatt aatcgtgggc
     5161 ggattgatcc ttctcctatt attgattact agtattattg tacaacgtag actacaaaaa
     5221 cagtattcct tgaagattca ttggaaacgt cgatttatca gtatcgctgt attattagga
     5281 tgtttttcgg gtgtcttttt cattaatcac aaaaattcgc cttctttctt gctgtttaat
     5341 ttatttaaag taaacaagac tttctttaac cagcaaggcg ccgtaaaaga taacggacca
     5401 gtcattcaat tcctaaacaa tatcgacatt aaaatcatgg aagaacctgc tggctattcg
     5461 aaagaaaaaa ttaatggaat aatgaaaaag tacaacacaa aggcagatga aatcaaccaa
     5521 acaagaagtg actggttaaa caatcagacg atcgttctca atttaagtga aagcttcagt
     5581 gatcctgcac gtgtccctaa tctgacagta cctacagacc ctattcctta catcaaaagc
     5641 attataacag aaggaacagg aggattgatg ttatccgtcg gttatggtgg cggaacagct
     5701 aacatggaat gggaaggact gacaggcttg agtatcagta acctgtctgc ttcgttagtt
     5761 acaccttata cccagctagt ggagcgtcag aaaataagtc caaatattac cgatctattc
     5821 gatgaaaaaa tagccatcca tccttttaca gctgctcttt atcgcagaaa acaagtgttt
     5881 gataaatttg gttttgacag gttctattat ttagacagtc cggataaatt aacttatacg
     5941 gataaaatag aacaaagccc acgcatatcg gatgattcag catacaaaga aaccttgaaa
     6001 gctttacaaa gcaatacgga aacgtcacaa ttcatccaat tatccactat gcaaaaccat
     6061 atgccttatg atgattacta tagtgaattg aactacactg ctgaaggaac tgctgtcatt
     6121 cctaatagaa gaaaagaatt acaaacttat atgcaaggcc ttcactatac cgatacagca
     6181 gtaaaaaaat tcatccaaga aatcgacaaa atcgataaac cgattacctt tgttttttat
     6241 ggtgaccatt tacccgcaat ctatagtggg aatgattcga aaaaatatgg attagaacag
     6301 caccagacag attatttcat ttataacaac aagtatagtc gtgaacaagc agctacgtta
     6361 aataaagaag ttgttgctcc taataacttt ggtgctatgg ctcttgagca tgcgaatatc
     6421 aaagtaactc cttactatgc gttgttgaca gaagtatcta atcatctacc tgcattaaca
     6481 attgatccta cagaaagctt gtctaatcgt tttaatggaa aacaggtctt tgttacagag
     6541 gatagtcaaa ttatccaaga aaaaaattta actgagaaac aaaaagaaat tctagaagat
     6601 tatcgtctga tacaatatga tcttgttgca ggtaaccagt acagtgcaac atgggcgaca
     6661 caaaaaataa aagactaaca aataaactga gcaaggggtt gtgataaaag acatgtcaca
     6721 accccttgtt tatctcatta atttgcttgt ccataaacga tttgattaaa gccatttttc
     6781 aattcattat tcgctgtctc gttgaagtaa agataattat cgggattccc ctgattttgt
     6841 tgtgtcacca atacttttgg ctgattttgc tgaaacccga atagatagac gtgctttttc
     6901 agatatggtt ctgtctctgc atcggaatag acggctgtga tttgatagtc tgcttgacct
     6961 gtcccacttt ctgaccattc tgcagaaaca ggcactccac caatagccat cttccactcc
     7021 ccgttcaaaa tagcttgagg gactttaaga ccatataagt ccacactcat ttgattcgta
     7081 taacttttgt attgttgtcc cattgtttgg ccccatttgg tcataaatgc ctctaattgt
     7141 gcggatttgt ctgcattcca cagtgtttgt tcaacttgtt cgctgctttc tgaagaggaa
     7201 gatgaagcct ctacagaaga acttgaagta gatgtttgag ttgtttcagt cgttttggaa
     7261 gactctgacg aagtagaggt atctgctgtt tctgtacttg ctgcagttga cttgctctct
     7321 gtgctagttg ttgtagtagt tgcaactgtt gctttcgctg tttcttttgt tgattctttt
     7381 ttcgattcat tcccgttggt acaaccagct aacaaaaaag ctagaccaat tgttactaaa
     7441 atcctttttt tcaattaaaa aactcctttc gattactccc acttctttat tctatcaaat
     7501 aagcaatgtg attttattga tttttcgtat tttctcatca atctttcaaa tcttctaaaa
     7561 actgagaagc acccgctgat gttgtctgta atacatcagc gggtgcttct tttattttaa
     7621 tcttcgtgat tttccaattt acctcacgct ttatatttgt atttgaagaa acatagactt
     7681 ttagtcatca tcgtcttcct catccaaatc ttgtcggaca gtttttcctt ggagtctttg
     7741 tacttggtag atgatttctt tattgaaact taaaagttca acaagcaatt gttgcaagcc
     7801 atatttttct tctttttcag ctaatttgat tgcccgtgtg ataaagacat tttggaaatc
     7861 aaaatctttt gcaaagtttt ccaacattgt tgaggctggt aaatatttgt tttgaccatg
     7921 ttcgtaaatt tttccataac gcatgacttc ttctaaagta gacggaacga tttcattttc
     7981 aatcactaac tcatgagaga ttttattgaa ccatctcgct tcttgatcag catattctga
     8041 aaaaagggta cttaattctt gcgaagatgt acccaaaaca tacaattgtg cttggatcaa
     8101 tttcatttct tctaatttaa aatttgctaa tacatgcccc accatggcgc cagcagttgg
     8161 tttatgatgg tcgatatctg cttgttttgt ttcttgaaca aatttttctt ccactatatt
     8221 cattgattta cgcctctttt acttttgctg attggacttc ataacctaat ttttccacaa
     8281 ctgcagctaa ttgttcaggg tggacttcat ctggatcaaa gtttgctttg attttgctcg
     8341 cattaaacaa aactttcgta ttgcttaccc caggctgttg agaaaccgca tgttcgattt
     8401 tttgcataca gcttggacaa gctagtgtat ctaattgcat aataattttt ttcatcgtag
     8461 ttcttccttc ctttttcttg atagtcttat tctagattac agcttccata aaaaaattga
     8521 ttcttatcaa gttttacgat ttttcttctt tgctttcgtg tccaaaatac aataaacgca
     8581 tcgcgttaaa gataacgatc aagatactag cttcgtgaac gaacatccca ctagccatgt
     8641 aaataaaccc ggcaaataag ccgttgagta aaaataagac gacaccaatg gcaatcacta
     8701 cattttcacg ggtatttaaa acagttttct tagccaagcg gtaggcatga accaacgatt
     8761 caaaattaga ttgcatcaag acaacgtcac tcgtttcgat agcaacatcc gttcctgatc
     8821 ccatagcaat cccgatatca gccgcagcca aagagggaga atcattgatt ccatctccaa
     8881 taaaagcaac gcgcaatcct tgttcttggt attttttcac aaattgaacc ttttcgtctg
     8941 gtaataattc agcatggact tcatcaatgc ctaacatatc agctacataa tcagcagtca
     9001 tctgattatc acctgtcagc atcactaact gttttgctcc tgcttgtttc aacttagcta
     9061 attgttcagc gacttctggt cgaatcacat ctgacacacc tatgatttga gtgatttttt
     9121 tgtcgattgc tacaataatg accgagctgc ctgtttgttg taattgatgt aaatcttgta
     9181 cttgctttgg tgtgagagaa agtttgtgag cggcaatcaa tttggtgttg cccgcacaaa
     9241 ctgtatgacc atcaatctca gctgtgattc cttgtccctt gattgtctga ttatctttga
     9301 ctgctaattg ttgaaaatca atgtctttct cctgagcata atggatcacg gcgcgcccca
     9361 gaggatgatc ggataacgtt tctaatcgag ccactaaagc taagctgtcc ccttcatcct
     9421 ctgtatagtt cttgatagtg gatacagcag tagttccttt ggtcaatgta ccagttttat
     9481 caaagacaaa agtatcgatt ttggaaaaag tatccattgc ttcaccgcct ttgactaaga
     9541 tgccccgttt cgctccatta ccgattcctg ctacattcga aacaggcgca ccaatgacta
     9601 aagcgccagg acaaccaaga accaaaacag tgattgctaa acgaaaatca cgactaatca
     9661 gaccaacaac caacgcaata agtaaaacgg ttggagtata atactgagaa aatcgatcaa
     9721 tgaatttttc cgcatgggat ttggtatctt gtgcttcttc tactaattcg ataattttgg
     9781 caaaggtcgt atcatctccg accttagttg cttcaatcgt cacgtagcca tctgctaaaa
     9841 tcgttccaga gaagacttca tctccactga ttttgacgac ttgtcgtgat tcaccagtca
     9901 ctgcggcttc atccgcatac cctttcccgt caatgactcg tccgtccaca ggaatagctc
     9961 caccagtttt tactaagacg atatcgcctt cttccacgtc atcgatttcc acttcttcag
    10021 tcgttccatc ttcttggaca acactagcag tagatggggc catctcggtc aaagccttga
    10081 ttgactcccg cgttttggtc aatgtttttt gttccagata gctaccaaac aaaaacaaga
    10141 atgtaacgat agctgactcc ttgtattctc cgatcataaa ggcaccaagc acagcaatag
    10201 taacgagtaa atcaattgag ataactttga ctttcactgc ctgatacgca tggatcataa
    10261 taggtatgac cgcaataata gaagctatgg ctagagcaat gttatagaac aagacaaaac
    10321 cagtaaattt tccgataaaa ccaaaaataa tcaacacagc tgataaggcg gtgatatgat
    10381 ttcgatgctc ttggatccac ttttgtaatt tcatctttct ccctcttttc ctttcattct
    10441 tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc aagtttagtc
    10501 aaaaaggcgg ttttcttttg gtagaataca ctacaaaaaa tagttagcaa gaagactcac
    10561 taaaagtgat cttgccaact attgtcgttt ctatcgaaat tttcattgaa tgattttcct
    10621 gtacagcata catcggagat ctatttcttt atctaagatt tttatcttgc taattttttc
    10681 ctttagtctt tcaaatccac gtcgttcata gaattgatac gtacagccac tatctgtaaa
    10741 aaggaatatt tcttttcctt tctctctttt ttcaaattct gttagcaact tggtaccgat
    10801 gccttttgtt ttcaaatcag gatttgcagc taaaaaaatg atctctccat ctggcgaatg
    10861 cttctttaaa tatgctgcaa gcatttgttc atttgctgtc tcgtaagctc ctttactttc
    10921 tttagcaaat atattttgta aaaaatcgaa tatcttaaca tatatttgct gtccaaaaga
    10981 acgatgtttc tttttttctc cttttatctc cgccagcaaa acacctgcca actcgtcctc
    11041 tgcatacaaa gcaagaattt gagttgctcg attcaactcc atatacaaaa aatatcttcc
    11101 gtaaagattc agcagaagtt cattttccat atatctctca aagttcattc cttcaacagc
    11161 aaaatagata gcttttttgt aatctttctt ttctaagggt ttaaaagtta gtttcatcat
    11221 ttcgcctcct caacttcaat gattgtgtta tacttacatt ataatagaca cagtgtctac
    11281 tatctataat caatcatcta taagtttgtc gattaagcga cagctttgct ttgatatgtc
    11341 ggataaggag ataggtaaat gaaaaagaca gatagacgcg tcaaaaaaac agaaaaagct
    11401 ttggctgaaa cactatccac attattagta aataaaaaga ttcaagcaat cactatacgc
    11461 gaactaacag aaacggcaga tgtacatcgt tcgacttttt atacacatta taaggatatc
    11521 tatgatctat atgatcaatt agaaagcaac ttttttcggg atctaaatga aattctctct
    11581 tatgatccag cccatagcta tgaggaacta tacactcgct tgattgacta tgtttacgca
    11641 aatacttttt tatatcgctt atttatcctc aaccatggtg atatttcgtt tcagaagaaa
    11701 gttaaacaag ttattgaaga aaactatctg aacatatggc tatatgaaga caagaaaacg
    11761 attatcacag aagaaatgag attctttact acgtaccacg tccaaggttg tctatccgtt
    11821 ctcaatcggt gggtagaaag caattacaag tactccaaaa aagaattatt agaactctta
    11881 cagaaactga atgatcacat agagaaaatc atgccttgaa taaaaacata ctaagcttag
    11941 ttaagaagaa ccgtaacctt ctcgtataac aactgaatct tatttagcca aattgtttac
    12001 tgaagatttt ttgagtaaaa tttctataat tcctacttat tgaaaagtag tttgtagtgc
    12061 tgcttctccc tctctaagtg atatatcttg ttgtaccaat caaattcacc tcatcattaa
    12121 taaattactt ttaggctaat ttattacaac ttataggttg aagctgtaac caatttatag
    12181 tcatattata tttttatcat ccagtttatt gaattcttct cgtttacctc aattcatttt
    12241 caaggggaat ca
//
LOCUS       DAHUWM010000085        12201 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHUWM010000085 DAHUWM010000000
VERSION     DAHUWM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.086"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            68..751
                     /locus_tag="NK788_001566"
     CDS             68..751
                     /locus_tag="NK788_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCH6769899.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="NK788_001567"
     CDS             753..1589
                     /locus_tag="NK788_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCH6769900.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="NK788_001568"
     CDS             1600..3354
                     /locus_tag="NK788_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6769901.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="NK788_001569"
     CDS             complement(3630..3971)
                     /locus_tag="NK788_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCH6769902.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="NK788_001570"
     CDS             complement(4039..6210)
                     /locus_tag="NK788_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCH6769903.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="NK788_001571"
     CDS             6379..7278
                     /locus_tag="NK788_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCH6769904.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVPSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="NK788_001572"
     CDS             complement(7349..8206)
                     /locus_tag="NK788_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6769905.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="NK788_001573"
     CDS             complement(8191..10893)
                     /locus_tag="NK788_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCH6769906.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="NK788_001574"
     CDS             complement(10906..12195)
                     /locus_tag="NK788_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCH6769907.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         3371 a         2600 c         2219 g         4011 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac cttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata a
//
LOCUS       DAHUWM010000086        12191 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHUWM010000086 DAHUWM010000000
VERSION     DAHUWM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.087"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(152..316)
                     /locus_tag="NK788_001575"
     CDS             complement(152..316)
                     /locus_tag="NK788_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769908.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            611..1939
                     /locus_tag="NK788_001576"
     CDS             611..1939
                     /locus_tag="NK788_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCH6769909.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2141..2974
                     /locus_tag="NK788_001577"
     CDS             2141..2974
                     /locus_tag="NK788_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCH6769910.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2990..3727
                     /locus_tag="NK788_001578"
     CDS             2990..3727
                     /locus_tag="NK788_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCH6769911.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3882..4808)
                     /locus_tag="NK788_001579"
     CDS             complement(3882..4808)
                     /locus_tag="NK788_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCH6769912.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5083..5916
                     /locus_tag="NK788_001580"
     CDS             5083..5916
                     /locus_tag="NK788_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCH6769913.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5950..6789
                     /locus_tag="NK788_001581"
     CDS             5950..6789
                     /locus_tag="NK788_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6769914.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6815..7375
                     /locus_tag="NK788_001582"
     CDS             6815..7375
                     /locus_tag="NK788_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCH6769915.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7574..9295
                     /locus_tag="NK788_001583"
     CDS             7574..9295
                     /locus_tag="NK788_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCH6769916.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9460..10602
                     /locus_tag="NK788_001584"
     CDS             9460..10602
                     /locus_tag="NK788_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCH6769917.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10618..11829
                     /gene="thiI"
                     /locus_tag="NK788_001585"
     CDS             10618..11829
                     /gene="thiI"
                     /locus_tag="NK788_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCH6769918.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2047 c         2531 g         3460 t
ORIGIN      
        1 tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt
       61 ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc
      121 tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata
      181 cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga
      241 cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag
      301 caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa
      361 tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac
      421 aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt
      481 atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata
      541 gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa
      601 ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca
      661 agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc
      721 tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac
      781 ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac
      841 attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta
      901 cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac
      961 aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga
     1021 tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc
     1081 ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa
     1141 attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga
     1201 cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc
     1261 attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca
     1321 tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga
     1381 tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac
     1441 agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat
     1501 ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg
     1561 ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc
     1621 aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg
     1681 agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc
     1741 aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt
     1801 tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt
     1861 gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc
     1921 aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga
     1981 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
     2041 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
     2101 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
     2161 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
     2221 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
     2281 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
     2341 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
     2401 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
     2461 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
     2521 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
     2581 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
     2641 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
     2701 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
     2761 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
     2821 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
     2881 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
     2941 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     3001 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     3061 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     3121 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     3181 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     3241 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     3301 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     3361 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     3421 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     3481 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     3541 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     3601 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     3661 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     3721 taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg
     3781 aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt
     3841 cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa
     3901 atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt
     3961 tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct
     4021 tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg
     4081 attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca
     4141 cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc
     4201 ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc
     4261 agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg
     4321 atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg
     4381 tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct
     4441 tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca
     4501 ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca
     4561 agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata
     4621 cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg
     4681 tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg
     4741 atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt
     4801 tctttcatgg cgatcgttcc gccggtgtgt agtacaagta ttttttttca ttttacagac
     4861 ttccttacga ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt
     4921 tgctatcagt gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa
     4981 atggaaaaga ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta
     5041 caagaaagca gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta
     5101 acatcgttta aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat
     5161 gcagggtttc tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc
     5221 tcagttgaga attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat
     5281 caaggaatca gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt
     5341 gcgcattttt tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta
     5401 gtccaattcc cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt
     5461 agaaagtggt ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc
     5521 gatgaatttc ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc
     5581 atcgatgagc cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg
     5641 aactttgctc tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa
     5701 gagtggcgta aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca
     5761 catgaagtca aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat
     5821 tctggtggtg atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca
     5881 gatggactca atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg
     5941 gcgaagtcta tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt
     6001 tcaaaagcgt tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta
     6061 tgcggtgtag caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa
     6121 ttagatatgt atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc
     6181 gaccaccctt ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat
     6241 cgacaaatcg tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga
     6301 caatcgattt ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg
     6361 atgaaaaaaa tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta
     6421 gagatattaa aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa
     6481 aagttagaac atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat
     6541 atcatcccaa aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa
     6601 attgaaatgg aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa
     6661 ggtgtaggaa ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac
     6721 tttgtgactc aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg
     6781 atttattagt caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa
     6841 agtagaaaca ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac
     6901 agaaaatgaa gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc
     6961 agctacttca cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt
     7021 agataaagca gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga
     7081 acaagtagat gcactgacag atatccttta ctttacttat ggttcttttt cattactagg
     7141 aatagtgcct gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc
     7201 agatggaaaa cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca
     7261 gatggaatat gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc
     7321 gattgctaag aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga
     7381 gtttcactat ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag
     7441 tgaatgcatg aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta
     7501 ttatttggat ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac
     7561 ggaggaacca taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt
     7621 cgccattgtc gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga
     7681 tgccttagaa aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga
     7741 tgttaaaaaa atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa
     7801 atggaatgac atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga
     7861 aaacctcaat gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc
     7921 tacaatggac caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg
     7981 ggaaacggaa gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact
     8041 gaaagaaaaa gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa
     8101 acagatcaaa gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga
     8161 ccctgttgaa gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc
     8221 tctgatgaaa aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact
     8281 tgaagaaatc agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga
     8341 agatcttgct gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct
     8401 tgaaaaaaca gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga
     8461 ttcgttatat gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca
     8521 atcaacgatt gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga
     8581 gctggatcac acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg
     8641 tttccaaacc gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg
     8701 tcaacacgaa attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt
     8761 agaagatatc gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg
     8821 cgaaaaagaa gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg
     8881 ttttgtagaa aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt
     8941 tgctactgac cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga
     9001 agaggtcaat cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac
     9061 acatgattta gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta
     9121 tcgtcataca cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa
     9181 agaatatcac tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc
     9241 tggtgctttc aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa
     9301 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
     9361 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
     9421 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
     9481 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
     9541 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
     9601 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
     9661 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
     9721 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
     9781 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
     9841 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
     9901 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
     9961 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
    10021 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
    10081 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
    10141 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
    10201 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
    10261 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
    10321 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
    10381 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
    10441 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
    10501 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
    10561 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
    10621 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
    10681 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
    10741 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
    10801 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
    10861 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
    10921 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
    10981 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
    11041 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
    11101 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
    11161 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
    11221 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
    11281 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
    11341 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
    11401 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
    11461 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
    11521 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
    11581 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
    11641 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
    11701 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
    11761 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
    11821 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
    11881 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
    11941 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
    12001 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
    12061 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
    12121 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
    12181 atcagaggtt g
//
LOCUS       DAHUWM010000087        11992 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHUWM010000087 DAHUWM010000000
VERSION     DAHUWM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.088"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(221..1423)
                     /locus_tag="NK788_001586"
     CDS             complement(221..1423)
                     /locus_tag="NK788_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6769919.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="NK788_001587"
     CDS             complement(1426..1620)
                     /locus_tag="NK788_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCH6769920.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="NK788_001588"
     CDS             complement(1667..2515)
                     /locus_tag="NK788_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCH6769921.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="NK788_001589"
     CDS             complement(2720..3070)
                     /locus_tag="NK788_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769922.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="NK788_001590"
     CDS             3665..4111
                     /locus_tag="NK788_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCH6769923.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="NK788_001591"
     CDS             4735..5727
                     /locus_tag="NK788_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6769924.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="NK788_001592"
     CDS             5743..6627
                     /locus_tag="NK788_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6769925.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="NK788_001593"
     CDS             6676..8283
                     /locus_tag="NK788_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6769926.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="NK788_001594"
     CDS             8284..9768
                     /locus_tag="NK788_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCH6769927.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="NK788_001595"
     CDS             9945..10937
                     /locus_tag="NK788_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6769928.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="NK788_001596"
     CDS             10989..11858
                     /locus_tag="NK788_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6769929.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3716 a         1960 c         2484 g         3832 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata at
//
LOCUS       DAHUWM010000088        11577 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHUWM010000088 DAHUWM010000000
VERSION     DAHUWM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.089"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(105..629)
                     /locus_tag="NK788_001597"
     CDS             complement(105..629)
                     /locus_tag="NK788_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769930.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="NK788_001598"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="NK788_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCH6769931.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="NK788_001599"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="NK788_001599"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCH6769932.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="NK788_001600"
     CDS             complement(2351..3031)
                     /locus_tag="NK788_001600"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCH6769933.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="NK788_001601"
     CDS             3157..4026
                     /locus_tag="NK788_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6769934.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="NK788_001602"
     CDS             4052..4567
                     /locus_tag="NK788_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769935.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="NK788_001603"
     CDS             complement(4614..5207)
                     /locus_tag="NK788_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6769936.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="NK788_001604"
     CDS             complement(5232..5411)
                     /locus_tag="NK788_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769937.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="NK788_001605"
     CDS             complement(5488..5997)
                     /locus_tag="NK788_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769938.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="NK788_001606"
     CDS             complement(6093..6473)
                     /locus_tag="NK788_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6769939.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="NK788_001607"
     CDS             6803..7552
                     /locus_tag="NK788_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCH6769940.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="NK788_001608"
     CDS             complement(7736..9013)
                     /locus_tag="NK788_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769941.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="NK788_001609"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="NK788_001609"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCH6769942.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="NK788_001610"
     CDS             complement(9935..10879)
                     /locus_tag="NK788_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCH6769943.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="NK788_001611"
     CDS             complement(10881..11567)
                     /locus_tag="NK788_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCH6769944.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3292 a         2458 c         2043 g         3784 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcct
//
LOCUS       DAHUWM010000089        11374 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHUWM010000089 DAHUWM010000000
VERSION     DAHUWM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.090"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..521)
                     /locus_tag="NK788_001612"
     CDS             complement(<1..521)
                     /locus_tag="NK788_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6769945.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFES"
     gene            complement(533..1225)
                     /locus_tag="NK788_001613"
     CDS             complement(533..1225)
                     /locus_tag="NK788_001613"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCH6769946.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1244..1528)
                     /locus_tag="NK788_001614"
     CDS             complement(1244..1528)
                     /locus_tag="NK788_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769947.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1541..2104)
                     /locus_tag="NK788_001615"
     CDS             complement(1541..2104)
                     /locus_tag="NK788_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCH6769948.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2332..2994
                     /locus_tag="NK788_001616"
     CDS             2332..2994
                     /locus_tag="NK788_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCH6769949.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3118..4314
                     /locus_tag="NK788_001617"
     CDS             3118..4314
                     /locus_tag="NK788_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCH6769950.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4415..5791)
                     /locus_tag="NK788_001618"
     CDS             complement(4415..5791)
                     /locus_tag="NK788_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6769951.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5828..6694)
                     /locus_tag="NK788_001619"
     CDS             complement(5828..6694)
                     /locus_tag="NK788_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCH6769952.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6783..7808)
                     /locus_tag="NK788_001620"
     CDS             complement(6783..7808)
                     /locus_tag="NK788_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6769953.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7826..8500)
                     /locus_tag="NK788_001621"
     CDS             complement(7826..8500)
                     /locus_tag="NK788_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6769954.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8684..9577)
                     /locus_tag="NK788_001622"
     CDS             complement(8684..9577)
                     /locus_tag="NK788_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCH6769955.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9651..11348)
                     /locus_tag="NK788_001623"
     CDS             complement(9651..11348)
                     /locus_tag="NK788_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCH6769956.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3374 a         2507 c         1956 g         3537 t
ORIGIN      
        1 gactcaaaat gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct
       61 ttgaaaatat ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag
      121 atatctgtac atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg
      181 tctagatctg ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc
      241 tcttgctcat agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt
      301 cctatcacat tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca
      361 tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca
      421 gtcagctttt tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca
      481 acaaaatcat atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga
      541 gatgtttgac aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt
      601 cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa
      661 atgggatatt gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta
      721 atgcttcagg gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat
      781 cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca
      841 tttcactgcg caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg
      901 gtttgtagcc aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga
      961 tgtcacctac ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt
     1021 tcacgccgta ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac
     1081 cggaacgtac gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg
     1141 cgcgttccca tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca
     1201 ttgcgccgat aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg
     1261 ctaaaaaaac cacacataac attaggataa caataataag agatttcatc aaaaaagagt
     1321 tccatttggt cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt
     1381 tgctttcttt acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg
     1441 cttttcgctg tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc
     1501 ttcgcgtaat aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata
     1561 gttcccagta ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg
     1621 cttgttgcgc ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg
     1681 ctaaaggatt ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg
     1741 caaaacctgg tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag
     1801 tttcttcttc taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga
     1861 tttttccagc tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa
     1921 gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac accaccagga tgaaatacca
     1981 gttctctctt cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa
     2041 tttttccgct atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca
     2101 gcatatcttc tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt
     2161 ctcaaaaact ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa
     2221 atccttcttt agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg
     2281 aataacgaaa agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa
     2341 cgaaattggc cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt
     2401 ttttcttttg ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt
     2461 ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa
     2521 actcactatg aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg
     2581 aatcaaacaa aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt
     2641 aaagcaatcg atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg
     2701 gtgaaagaac caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat
     2761 ctagttgatt tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag
     2821 acctatgaaa aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca
     2881 caagattatc aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt
     2941 gcgaaacaga gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt
     3001 tgttcataaa agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc
     3061 taaagtagag acattcatgt aagaatcgag aacagattca acaaggagga attttttatg
     3121 gatcataaac cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta
     3181 ttgaacaacc cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt
     3241 gatcagctcc aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc
     3301 caagaacaag ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc
     3361 agctacggat cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat
     3421 gtgcaagcac aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc
     3481 caagaagcca atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc
     3541 gggaaggtca aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa
     3601 attgacaaga tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg
     3661 atgttagaac agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc
     3721 gctgctggag aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa
     3781 agagcagaag aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc
     3841 ctagatcgtc tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag
     3901 caagcaccac aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa
     3961 gcatctatcg ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg
     4021 cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta
     4081 ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa
     4141 cgtggaatcg tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc
     4201 caagagacat tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag
     4261 cttggacaca tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc
     4321 tttcgcttgt tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt
     4381 tttcttatct ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc
     4441 catagccata gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc
     4501 atgtcacagt ttgcagacga agcatcaata caatacaagt caagatggag acgattggca
     4561 atagcggaac gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca
     4621 acttcagtaa gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt
     4681 ccgctaatac attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat
     4741 aagtcgcttt tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta
     4801 agccatcctt gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta
     4861 ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta
     4921 catacctcat tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc
     4981 ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga
     5041 ttcctcttgg cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat
     5101 caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa
     5161 aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca
     5221 acgcgatgat accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg
     5281 cttcaagcga cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt
     5341 atgtaccatt ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac
     5401 ttttcaaaaa accttgcata tagccagacc atccggaagc aacagaggaa accgctagta
     5461 aaaattcccc gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat
     5521 acagataggc atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta
     5581 gtccagacaa aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag
     5641 cattttgatt ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa
     5701 ttccaagcat gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga
     5761 gattgatctc tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa
     5821 acagcgatta aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc
     5881 aaccaacaat aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc
     5941 cacctaaagt agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa
     6001 gtgatttgac tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca
     6061 tgtttttcca cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta
     6121 agatcattcc gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga
     6181 cgatgatggt atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac
     6241 caaaatcaag cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca
     6301 aatacgcacc gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt
     6361 gtgtccattc atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag
     6421 tagacaatgg tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt
     6481 gtccaacact ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta
     6541 caggactaac aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta
     6601 gtgtttgttg attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg
     6661 ccaacatagg gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga
     6721 taagaaactt atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt
     6781 ttctaacgtt tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct
     6841 tttccttcag ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct
     6901 aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat
     6961 cccttatcaa agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact
     7021 gaaaggctga ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc
     7081 gtatatttga tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg
     7141 gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa
     7201 tttgcctgac tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg
     7261 tagtcccgcg agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg
     7321 atttttgata attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc
     7381 gaacgaacta gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca
     7441 atataatctt tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca
     7501 cggatcagct gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc
     7561 aaataatttt gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc
     7621 ttgctcaagt aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa
     7681 taagcaattg tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc
     7741 aatgcaataa aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa
     7801 aaattcatct taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt
     7861 cgatatatcc ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg
     7921 tcaatgtatt gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc
     7981 gacttaagat ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc
     8041 tcaaatcaat gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc
     8101 cattgtggct catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg
     8161 cattgatttt tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc
     8221 ccatgttcat agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg
     8281 gtactttcga tacttcacgt attttttgac accaataata gccatccaaa acgggaagat
     8341 taatatctag taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga
     8401 aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc
     8461 gaatgatctc ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc
     8521 ttcttgctac tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg
     8581 tcattttagc aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc
     8641 caagcagctt aaatttatta tgttattctt aataattggt ctattatctg ttatttttct
     8701 ttcgttgttt ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta
     8761 gcaatatacc tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta
     8821 tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag
     8881 tgttcacggt gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg
     8941 ctaatgtatt tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca
     9001 tccgaccata cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa
     9061 agagtttttt ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc
     9121 cagttggaaa agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg
     9181 tttcatcaaa taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat
     9241 gttgttcgta atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat
     9301 ctttgacttg tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt
     9361 catgcactgc taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat
     9421 aattctcgat ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg
     9481 taaaaactgc agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga
     9541 tttgggtttg aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa
     9601 aaactgcagc aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca
     9661 gacttcattt tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt
     9721 agtaagaatg ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc
     9781 ggtactcttt ctggccctgg attttctact cctacgatct gaatttcttc attatagctt
     9841 ccataagaga tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct
     9901 ggcgccatgg tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat
     9961 ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct
    10021 gctcctgatt cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa
    10081 cggtcttcat tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca
    10141 tatttttcaa actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca
    10201 taatcaaatt catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc
    10261 gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca
    10321 tctgctaatc tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc
    10381 acgacaaacc cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc
    10441 tcgccggctc gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct
    10501 gtgattttgt aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa
    10561 ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa
    10621 atcgtttctt gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct
    10681 acattcatct gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta
    10741 ttgtatgtgt ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc
    10801 actggcatca taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt
    10861 tctaagaaaa gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta
    10921 gagaataccg cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat
    10981 tttaaaaacc aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta
    11041 agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc
    11101 gttacgtcaa acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa
    11161 accaatccgc tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta
    11221 atcgtcactt cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg
    11281 aaaatcccta ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt
    11341 ttattcattt ttttctcccc gttctttttt tatt
//
LOCUS       DAHUWM010000090        11236 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHUWM010000090 DAHUWM010000000
VERSION     DAHUWM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.091"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(89..1552)
                     /locus_tag="NK788_001624"
     CDS             complement(89..1552)
                     /locus_tag="NK788_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCH6769957.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1616..4633)
                     /locus_tag="NK788_001625"
     CDS             complement(1616..4633)
                     /locus_tag="NK788_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6769958.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4784..5623)
                     /locus_tag="NK788_001626"
     CDS             complement(4784..5623)
                     /locus_tag="NK788_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6769959.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5768..6013
                     /locus_tag="NK788_001627"
     CDS             5768..6013
                     /locus_tag="NK788_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769960.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6076..6498
                     /locus_tag="NK788_001628"
     CDS             6076..6498
                     /locus_tag="NK788_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769961.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6592..6909)
                     /locus_tag="NK788_001629"
     CDS             complement(6592..6909)
                     /locus_tag="NK788_001629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769962.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6936..7142)
                     /locus_tag="NK788_001630"
     CDS             complement(6936..7142)
                     /locus_tag="NK788_001630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769963.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7123..7305)
                     /locus_tag="NK788_001631"
     CDS             complement(7123..7305)
                     /locus_tag="NK788_001631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769964.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7319..7669)
                     /locus_tag="NK788_001632"
     CDS             complement(7319..7669)
                     /locus_tag="NK788_001632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769965.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7695..7997)
                     /locus_tag="NK788_001633"
     CDS             complement(7695..7997)
                     /locus_tag="NK788_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769966.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8000..8233)
                     /locus_tag="NK788_001634"
     CDS             complement(8000..8233)
                     /locus_tag="NK788_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769967.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9219..9541)
                     /locus_tag="NK788_001635"
                     /pseudo
     CDS             complement(9219..9541)
                     /locus_tag="NK788_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9664..10779)
                     /locus_tag="NK788_001636"
     CDS             complement(9664..10779)
                     /locus_tag="NK788_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCH6769968.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10785..11183)
                     /locus_tag="NK788_001637"
     CDS             complement(10785..11183)
                     /locus_tag="NK788_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCH6769969.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         2946 a         2326 c         1736 g         4228 t
ORIGIN      
        1 aaacgaaaat gagagataca aatatatcaa atatttaaaa agtaaccaca ttttctatac
       61 cactccttat cagaaacagc cttattcttt agaataagac tgtttctggg ttgattaatt
      121 tgtcttttgt taaactacca tctaaggcaa gttctaaatg taaatgtggt cctgtactat
      181 ttcctgttga acccacataa ccaataattt gtccttgttc aactgtttgt cctacgttca
      241 cgcaatattc ctgttggtgc gcataaagag ccacacttcc gtcttcatgc tcaattgtga
      301 cataatttcc tgacaaagta tgatacgaag cttctatgac cgttcctgcc ttgctcgcat
      361 agattggttc tccttgattg gctgccatgt ctaatcctct atgaaattct tctcctcgcc
      421 aaccgaagga actagaaatt gaataatttt ttagaggtac catatagcct tctttgctta
      481 gttgtggacc agctacttct ttatcatatt tgattaagtc ataggtttcg attaacccat
      541 ttaatttttt atcgtagcta gtgtctgttg cgtatcttcc tgttaaaaat ttagtcgctt
      601 cctgataggt ttttgcctta cttttccaaa caccatcata aaaagaggta tttccaacta
      661 agccttcttt taacagtttg gcatagtctt ttaagctttc ttcatagctc tcatattgcc
      721 gaaaagttgc ttgggtcgta taaagtgtcc cattgcctaa atcttcttgt gtcgcaaaag
      781 agacgccctt tccgttatgt gtgcctttga ttccaaatag attgtagttt ggtgcctgag
      841 ccaattgact ttgtccgctt gcggattcta aaatggcttg cgcgatcata acagaagcgt
      901 ataaatcatt ttcttgtcca attttacgag cagattcacc gattttacgg ataaacgttt
      961 ctacagacgc gtctttttca aaatgaatag tcccttcgtt atcttgtgtg ctggaaggga
     1021 ctaattcttg tatcggtgcg cttggtgctt ggtggtagtt ttttgtctct tctgaaccga
     1081 ttgattgtgc ctgcggtacg tcattcatag gtacagtcac actctgttca ggtgcctttt
     1141 gtgggcgttc cgtttttgtt ggcggttccg cttctgtttc ggtacttggt ttttgaggag
     1201 tctgtgagct ttcatcagaa ggttgctcat ttggttcttc tgacggttcc tcctcaggtt
     1261 gttgattgtc ttctggtttg tctggttgat tcggtgtttc tggttgttct tcagaacctg
     1321 ttggtggagc gttcatttct ggattatctg gtgtatctat tgtatcttca gtcggtgttt
     1381 ctggttgatt ttcttctcca gtggaatttg ttgtcgtttc ttcagtgatc gttgcagatt
     1441 gcgaatttgt cagttgattg ttgccttgtt cattagccaa aaccgctggc gtatacccaa
     1501 atacttgacc tactaataac aaagcagata aacatttact cgtttttttc acaagattgc
     1561 ctccattctt caatcaatca tctagagaca gctgattgct gtctctagtc atactttaat
     1621 tgctttgttt cattttacga cgaaccagaa cagcccccac accaacaaaa agaaacagtc
     1681 ccaatgcaat taacacatcg ttcactgttt ctcctgtttt tggtaagctt tgttttggtg
     1741 tgctcgtcgg cgtttgtttg acaggggttg tcggcgtttc ttttttaatg ccttttgctg
     1801 tcaatgtttg tttttcattc ttcaaatcaa agtgacgtgc ttcttcctct ttatggtctt
     1861 tatccttata gacaatatga gttgcgacca atttctcccc gtcttttaac gtgcctgctt
     1921 tgtagtcaaa ggatacttta aactcttcag acgctttaga aactttacgt gcagtttcta
     1981 ccgtaccaac gactgtatct tttccatttt tatcaatctt atggaattga gtcacccagt
     2041 atttggtttc ccctaccgta gattctggaa attcttgcga tacatgatcg atcaactctt
     2101 ggtctttgtc tggatcaaat gtttggtcgc cattcactgt aataaagcgt gtttcgattt
     2161 ttggttcttt cgcagtcact gtttgatctt tattggttaa atcaaaatga cgagcgaatt
     2221 cttcttcctc ttctttgtcg ttatagacaa tgtgcgttgc gacaatcgtg tcaccgtctg
     2281 aaaccatgtc tgctttgtag ttgaacgtca catcaaatgt ttctttttca tcggtcactt
     2341 ttcgtgtttc ttttaccgta tctaatacag tgacttttcc atcggcagta attttatgga
     2401 attgcgtcac ccaatatttc tctgtcccaa ccgcagattt agggaatact tgggacactt
     2461 tatccactaa ttcttggtct tttttcgggt tgaaatcttt tccacctgtc gttgtcacaa
     2521 acaaagtaga aatttctggt tggtcttctt ctggaaccac gatttcttcg tttttcacag
     2581 taattggttg tacttctccg tctttgaccg ttactaaaat cgtttctgtt tgtcctgacg
     2641 cttgcccata atgctcaggg aacacaattt ccgtaaattt atacgtaccg tctggtaatt
     2701 ttagtttttc tggttcttct tttgtttcat acacaaagga cacattttta ttatgtgcag
     2761 tcataccttc aatcaggaat ttcgctcctg gtaattgtag atgattgatt gaatcgactt
     2821 tattgaagtc tacttcaccc gtttttaaca agttgtcaat cacatctaat tcaaacgtaa
     2881 gcacaggcgt cgtgttatcg ccataggcca cagagaacgt ggtttgttct tcagaaggtt
     2941 gacggtagtt gtctggcaca tttttttcaa tcaattggta tttgttttga tcgccattgt
     3001 agaatttttt gtttgccttc cagtgtccgt ctttgtcagt ttttactgaa tccaagactt
     3061 ctcctttttt cacaaggact gtttctttat ctggcgcagt aatatctgtt ttggcgataa
     3121 tatcaaattc gatattttct gcaggaacgg tatcataaac aggtttgcct tgattccagg
     3181 attcttccgc attatagact tctttgactt tctttccttc tggaatgaac tgttgtaagt
     3241 cattttttgc agttaacgtc acttctttgt ttggttcaat cgtaatcgta tgagtttgtt
     3301 tgtctagaat atacccagct ggtgctttga cttctttgac atccaattta ttttgaacat
     3361 attttccgtc tttttcatag aatgcaggga tttcttttaa ggcgatttcc cctttggcat
     3421 ccgttgtata ttgttttgtt tctcccgtat catgacgttt gatttcatac gtcacatttg
     3481 gaactttttt ccctgtgaca gcgtctagtt tggttagttt gaagtcgcca ttttttagaa
     3541 ctttaatagt tagtccagct ctattaggat ctggcacata aagctttcct agattttgag
     3601 caacaggatc tacataggca aaacttgaac ctacccctct agaatttgga tccagtgaag
     3661 aaacagaaat tcgtccgtca tttgagtttt gatttgcttt tataaacaaa ctatttcctg
     3721 attttcttac ttgaacgtta gctgtgtttt caacaaccga cccatcccat tgagaaaaca
     3781 caccttttgt atccttaact tctacttctt cccctacttt aaccgtcact gtttggctat
     3841 caaaagaagg atcttcaagt aaattgttta tttttctatc tacagtctgt ttgaaagctt
     3901 cataattaga ttgtccaata gttccataat taacaaatga accacctaaa acttcccaaa
     3961 tatatgtttg tgctaataca tagttatcca ttgaaccact agaatagtag ccaaaataac
     4021 caattaaaga aatctttttt ctaatagcac ttgtcgtaat taaattttcg agtggtgtca
     4081 catcatattg aatacccgca ctcgtgattg cgccttcttg aatgcaaaaa acatcttctc
     4141 cgtcaatttg ccagcgaacc atgtaatcat gagtatacat tgttccttca ggagtaattc
     4201 gagtcatatt cacatcttta cgaccatttt cagtcaatgt tagattggta gcacgtgtct
     4261 gtgcattctt ttccaagatt gcttgccatt cttcagattc ttgcgccaat cgttcttgtt
     4321 cagaaaaaga agtcgctttt tgaatatctt caatggttat aaagtatttc tcataacctt
     4381 cagggacctc ttgagtcatt acattgggcg gagaaacatc ttctgtcgtg tcctgcgtag
     4441 ttgtctcttc tgttgtttca cttgtggttt cagtactgat cgcttcttca ctactttttg
     4501 attcttcagt cttcattact tcttcagtag aatttgattc atttatcgtt tctgcaaatg
     4561 ccgttacaga tcctaacatg ggattgagaa atgtaacgaa aattgccaac caagttgcca
     4621 ttcttttaaa catatttttt tcgttccttt cattttattt ttcagtcttc taaagtaatt
     4681 tcttatggac ttcgacaccg aaacattttt atcctcctca aaacaaaaaa agactagaac
     4741 ttataattct agtcttcatc aaccacttac ttttctttta tttttagtag aaatctacag
     4801 tgtgtttttg tgacccgtca ctgtaaataa taacatccac cataaaacct ttgttgtacc
     4861 actgcgaatt tggatcgcta acctctgcat tagcccaatc ataggcttca tctacagtgt
     4921 taaatacctt tccactatta ccaatctctc caactacaat tgttggctct tcaggtgtcg
     4981 ttgtgccacc atttccgcct tgatcgcctc caccaggatt gcttggttct tcaggcgttg
     5041 ttgttcctcc acctgtgctt gggttagaag gagttgtttc tcctccagta tttccaccat
     5101 tacctggttg tgtaggtgtt tgagtagtgc caccattgtt ttctggtgta cttggcgttg
     5161 ttgtaccacc agttgttcct gtttgatctc cagctggtgt tgttggtgtc gttgtatttg
     5221 gtgtcgtttg tgtatttgaa gtcgttcctt gtcctactgg ttgattcgga tcaactttgt
     5281 ctttctcttg aatcactgtt tgagccacag tttcacctgc gctgttttgt acacttgcta
     5341 cattgccatt aaatactaat ttatttcctg catagattaa atccacattt gtaatcttat
     5401 tcatgtccgc taatttttgg acagtaatat ttgttgcttg actgattgcg cttagggtat
     5461 ctccccagac aattgtgtat tcattccctt taatttctgc tttgatttgt tccgccgaac
     5521 gaggtgtcca aactttgttt tctgctgctt cagcattcgt tcccacagca agactaactc
     5581 ctccgactac gattcctagt gccaatactt tcaacatttt cattgttttt cgcctccgat
     5641 ttgaataaat ttatttacaa aaacatagta ccaaacttat gtctatcagt cgatatattt
     5701 tttgtagatt tttttatttt ttgatatact aactttggta ctaaaataac taaaaggaat
     5761 gtcacacatg caacttaata atttaaatga aatacatgat tgggctcaaa ataatttact
     5821 taatcgtaaa caagctgcca aaatcactgg acaaaactat gatgcttttt ctagctcttt
     5881 acgtagaaat aatatcaaac catttctaga aataggcgac gaacctggtc cttccatcat
     5941 tcgcttatat ctaagagaag atattgaaaa atacgcaaaa cagctcgaag aaaaaagaca
     6001 gaaacgtcaa tgaggcgttt tctgtcttgg atttacaagt aaaacaataa taaacacttt
     6061 tacaacggag gttctatgaa taagaaatac attgtttcag ttggttcaat cgtactgatt
     6121 gtactcgcta ttattgcttt ttctgtatcc aacaaaacag aaacacagcc acaaaatacg
     6181 gttcaatcga ctactacaag tcaatctagc gatcattccg tctatccttc actaaatagt
     6241 ttaaaagaaa cgcatggaca agatttaatt gatcttggtt cttctcaaat tactactgtc
     6301 ttatttgaaa agaaaaatat tgcgtttatt actcctaaag aaggaactga ccttgaattt
     6361 tctatcaaag taacaaatac caaagaaaat ttattctcca ttcctgacat tcaatatacc
     6421 cctaacaagt taaagattgg ggatacgttt ggtttggcta aagtaaatga acaatacttt
     6481 gcttactcac tgaaataagt ttctaatagc aagaggaaaa accctcttgt tatttttatc
     6541 acataatcat tttataaaaa cgcttcgaaa atatcaacat ttttgaagaa actatttttc
     6601 taatttctca atttcttcca agggattttc gctaatgacc catacaggca cagattgctc
     6661 tcgaggctcc ccttcttcta accaccaacc tgcataatga acttcttcct cctcagcatt
     6721 accatgatac caaccaacaa aggattcatc tacaacgaat gaaatttctt cttctggatc
     6781 aaataattca tttttccatg cttcaattgc ttgttctttg gtgtactttt gtttgttaaa
     6841 tacaccacaa atttcgtcat ctacataaaa aatttcaaaa tcaaaattac tcgttccttt
     6901 tactggcatc ttaacttttc ctcctttgac tcacttcaat agatatacca ctcatgttca
     6961 ttccattgtt tactagttaa ttggagtttt atcttagggc tttgtgcact ctttccaaaa
     7021 acttgagcgt cgtttttttc ttctaaccaa aaaaggtaat ttcttacatt cggtactaaa
     7081 tgtttttcct gcatttcttc atgaaaaaca tataattctg cttcattttt caaccaattc
     7141 attattcatt ccctcattca cttcttcata ccgccattca gatagtgaga tttctttttg
     7201 tttctccact tgtcctataa aaatcgctgt attcaacaag ctgatcacac ttaataataa
     7261 agcaatgaaa ctaatggtta cacttactct ttcacatttt gtcattttgt ttccttcttt
     7321 actcgtttgt tatttcttgt aataggtcta ataactcctt caccgaaatt tgcaaatggt
     7381 tcgcaaaaat ttcaataaaa gcactcgcac acattaccag accttcaact gatatatcac
     7441 tgcgacaata aaattctgga gaaacactga tttctgattc tttcttacaa ttgaacgaaa
     7501 aaactatttt tgaatcaaat tccggtaatt cttccacaac tatgttttgg gattctacca
     7561 agaaagggag tgcctgttct aatgcttctt ctggtgtttg agcagataga ataattgctt
     7621 cttgttcttt tatttcattg ttctcaaata ctttataact ttttttcatt gttagttcct
     7681 ctttcattcc tctctcaatt agctaagccg ttactggtac gccattctct aaatttagta
     7741 tcgctaatcc ttaatgctcg tcgaatatca atcattcgat aacctattgc acgaagttct
     7801 ttatatcttt tagcagttag cgtctctaac gtcatttcag gcaatgggtt cgaatcaaaa
     7861 atagaattcg aaccgattat ttgggccaat tgttgtactt gtaacagctc ttctgaactt
     7921 aattcagtga ttgctcgatt gcctatcatt gcatacattt tctgtcgaat atacttcttt
     7981 tcttgtttag acaacatttc tatccactcc tattttcatt atcatttgtt acctccactt
     8041 cagtaaatcc acaaataaat gaattcatat tccaagtacc attcccttca actacaactt
     8101 ttctcctgtc ctctttcgga tattcaagaa tcagaccgtt taccaatatg tttttcacta
     8161 ctactaattt gtttgtatac cgtggaattg ctgttccgat atacttcact ttatttccta
     8221 cgctaattgt catttcctca ctcctattct tttttatcca aaactttttc ctgtcctatt
     8281 cctgccaacg aatatacttc actgcatgct taaacaaaac aaaatgttgc cctgttttat
     8341 ttccgtcttt tacttgttcc acatacagtt catatttggt cacacctttt aaaacgccag
     8401 atactttttt tcatccattg tcacaattag ttttttcatc aatcgattga tactgcactg
     8461 ctcatttttc ttgttcatat agtattcctt tctttttcaa aactagcctc ttttgtttac
     8521 tagttttcat caatcatcaa cgatttaaca gattttttct ttcgcagggt ttctagcgtc
     8581 agcaacccca aactctaaaa aagtgccttt gctcccttga aaaacgagcg gacgattgta
     8641 ctcgtcatca tatgtttttc atactacctg atccacgcaa ataacctttt tttgttctgt
     8701 gccacaccca tccatacgta tcgcttgaga ttcaccatac ttttttaggt ggtttatcct
     8761 tcctaagtcc ctgttagaca cgctgattaa cttagaaagt cctgtttgta tccaattttc
     8821 aaagatctcc caaaagaaat aagtaggttt acgtgtatcg cttctgttca aagtacctgt
     8881 ttgtacttga gtatttctac tcccctcgtt caagggattc cttccaccag ctagcttttc
     8941 ctttcatcat caaatgttcc tgaaaagcat ttacgtttcg tgtccgtgtg cttacttatt
     9001 cttttttcga tagcgattat ccgctcgcta ctcgctattt tatactttgt ttgttagctc
     9061 aaataaaaac taacaaacaa aaaaggctag tttctttccg ttttattttt ttaactgtcg
     9121 ataaatagtt gttagtttaa ttccaagttc taaaaccaat acgcccaaca aaaccacagg
     9181 aaaaacaatt ggggcaaaca agtaaaatcg gatagttttc atgtcttccc tctttctttt
     9241 tggtgtcgct gtggtcgttc ttgccgttca atacttcgat acatttttta ttcattattt
     9301 gctcgttttg ttttcctctg gactgctttt cttgcttttt gaatacttta tacgcttttc
     9361 gtgcctgaat taataggtct tgtcggcgtt tcattcgtga gaaaacaata tttgaaacca
     9421 tcaaatcctg gttcgaacaa ccacgattcg caataatttt cccatcaaaa aaaatagttt
     9481 tcacttgaga aaaaaacagt tggaaatctt gttttgttgc taattttttt gtcaaaatca
     9541 ctgtgctttt ccctgttgaa tctacaatga ttcttcgaac aatccgattc gataacttcc
     9601 aacgttgccg attaaacaat gttacttcac aacaaatatg acgtaagtga ttaggggtca
     9661 ttttcatgtg tccaattcct ccaaccaatt atgcatcgaa attttgggca attgtgacag
     9721 cccataaatc tgcgtttcca ggtcttcata actacaagaa cgcttggctt gaagtaatga
     9781 atattctgct tgatctgata gttttgttgc cattggatct agattcaatt ctcttacaca
     9841 acattcccaa aaattcaata gcgctctaaa ataaaatcct tttcgattct gaattgcttt
     9901 tccttcatga cgagcaattt tttcattttg aatacaccgt tgatacgttt tgcttaaatc
     9961 ttctgcaaat gtttccccta aaattcgata ctcgcttcca tcagataaac catgtgtttg
    10021 attatacttt cgttgccgct tttctataat tcgtttactg tcgtatatca tgtttagctg
    10081 ctctttcgct tcacgccatt cagctgaact gcgaacaatc catgtcaaaa catctttcgg
    10141 aacaaacgta ctttcgctgt aatgagcaat aaactctttt tgccaatcat cagccatttc
    10201 aacaggacta gtcgtatcga tttttgtatc tagattttct gtatctagat tgttagaaga
    10261 attattaaat aattgtgcag aattttcaca tggatttttt tcgtcaaaat catcgaaaat
    10321 cgctaaatta tcaacagatt cctcctcttc ttttccttgt gcagaatcta cactggttga
    10381 gtcaacagga cctaatgaat acacatcatt ttctgtcgta tctaattctc ctagatacaa
    10441 ccgagctttg tttctttttc ctgtttgttg atcaaatccc atatcctcta tttctagtag
    10501 ctcatattct ttcaattctt ttttgattgt attaacagaa cgtctgcaac aattcaatgc
    10561 attggctaat tcttgttccg taaattcaaa ataaacaaaa ccttgactat ctacttgatt
    10621 tcgttgaatg gctaaatcta agcgtgaccg aaaaaccata tacgcgattt ttgctttatc
    10681 agacaatgag cgatattttt cacttgtaat aaataccttt ggaaattgat agtataattc
    10741 attgtattta cgagttgcgg taaatctttt tgttgccatc gttattacac ctcatcttgt
    10801 ccatatatta agaaagaatt ttggtgttgg tagacacgaa catcttccat tgtagcaatc
    10861 aattctttta gataaattcg accgaattca attgttagta agtcgtaatc ttctaattca
    10921 tttttgattt cttcaatttc ttcttcagac acattgagta aaatggctaa ttcttgattg
    10981 gtataagaaa tatagacatg tttcttttca tcaatccgtt tttcaattaa ggattcttct
    11041 aagtactcat tcagtaagct ataggcgtat tttgcttgtg cagtcatcgc ataatatttt
    11101 gacgatttta taaagaatag ttttggcata tgaaaagttg cttgtgtcag cacatctgtt
    11161 gcgcgaaatt tgttttttac catatttttt cctcctagaa aaacacgcaa aaaaagctct
    11221 gtataaatcg aattac
//
LOCUS       DAHUWM010000091        11216 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHUWM010000091 DAHUWM010000000
VERSION     DAHUWM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.092"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..395
                     /locus_tag="NK788_001638"
     CDS             <1..395
                     /locus_tag="NK788_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769970.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="NK788_001639"
     CDS             398..1228
                     /locus_tag="NK788_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCH6769971.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="NK788_001640"
     CDS             1244..2095
                     /locus_tag="NK788_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6769972.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="NK788_001641"
     CDS             2092..2451
                     /locus_tag="NK788_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCH6769973.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="NK788_001642"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="NK788_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="NK788_001643"
     CDS             2624..2929
                     /locus_tag="NK788_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769974.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="NK788_001644"
     CDS             2929..3240
                     /locus_tag="NK788_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCH6769975.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="NK788_001645"
     CDS             3237..3410
                     /locus_tag="NK788_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769976.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="NK788_001646"
     CDS             3407..3823
                     /locus_tag="NK788_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCH6769977.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="NK788_001647"
     CDS             3823..4491
                     /locus_tag="NK788_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCH6769978.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="NK788_001648"
     CDS             4488..4865
                     /locus_tag="NK788_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769979.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="NK788_001649"
     CDS             4862..5161
                     /locus_tag="NK788_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769980.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="NK788_001650"
     CDS             5151..5309
                     /locus_tag="NK788_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769981.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="NK788_001651"
     CDS             5403..5819
                     /locus_tag="NK788_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCH6769982.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            6379..6567
                     /locus_tag="NK788_001652"
     CDS             6379..6567
                     /locus_tag="NK788_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769983.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            6557..6817
                     /locus_tag="NK788_001653"
     CDS             6557..6817
                     /locus_tag="NK788_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769984.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            7150..7722
                     /locus_tag="NK788_001654"
     CDS             7150..7722
                     /locus_tag="NK788_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769985.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            7723..8034
                     /locus_tag="NK788_001655"
     CDS             7723..8034
                     /locus_tag="NK788_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCH6769986.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            8025..8285
                     /locus_tag="NK788_001656"
     CDS             8025..8285
                     /locus_tag="NK788_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769987.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            8446..8805
                     /locus_tag="NK788_001657"
     CDS             8446..8805
                     /locus_tag="NK788_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769988.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            8802..10445
                     /locus_tag="NK788_001658"
     CDS             8802..10445
                     /locus_tag="NK788_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCH6769989.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            10480..>11216
                     /locus_tag="NK788_001659"
     CDS             10480..>11216
                     /locus_tag="NK788_001659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6769990.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDF"
BASE COUNT         4092 a         1619 c         2482 g         3023 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 gaattttaac tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat
     6061 tatgtcactg tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt
     6121 tcgattcctt gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg
     6181 cgcaatttcg tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta
     6241 gattgagttt tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa
     6301 acgtccctgc ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa
     6361 aagagaggat tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat
     6421 ttttatgaag tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg
     6481 aaggctacgg gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg
     6541 cttgcggagg tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg
     6601 tgaaagaatt gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt
     6661 catagtctgg tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa
     6721 aggagcaccg tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac
     6781 ttatctcaaa aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac
     6841 ataaaggagg ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc
     6901 gaaccattgg tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact
     6961 ctattgtcga aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat
     7021 attgcggtca tggctattgg aaagacgagt atatccaata caacattgag aggtacatca
     7081 aatgaatgat aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg
     7141 aggaaaacta tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc
     7201 aatctagctt acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct
     7261 ttagatgcca ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct
     7321 gttcttagtg ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt
     7381 actggatatg agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag
     7441 atcaaaaaga ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa
     7501 atcaaactaa gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat
     7561 gagatgccga taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc
     7621 caagtaccaa aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag
     7681 tacttagata gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt
     7741 aaacaagcaa gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc
     7801 ttagagcgag atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa
     7861 catgattcaa ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct
     7921 ctagatataa acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt
     7981 atgaaatatc gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa
     8041 cagcaaacag agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg
     8101 ataagttggt tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat
     8161 ctatcaacaa tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg
     8221 tagacattga ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg
     8281 gctgataaat cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt
     8341 tgagaagaaa taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg
     8401 ctattttcac acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa
     8461 gagagaaata ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt
     8521 gataaggata aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt
     8581 cttcttgatg aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag
     8641 tcagaaaatc aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga
     8701 gaccaagcat atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc
     8761 gaacctacaa aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat
     8821 tctattttga tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa
     8881 gaattcagtt aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg
     8941 atgaagaaat gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta
     9001 aatatcaaaa atttatcaca cctttcattt tctgctatca aaaagaggac gacgaggtgt
     9061 tttttgatga aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag
     9121 caagagactc attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta
     9181 cagcgaattc tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa
     9241 tgcgcaaact ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta
     9301 attcgatttt tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct
     9361 gcttagaatt tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta
     9421 gtgggctagg gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta
     9481 gagaaggttt ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca
     9541 agaatgatcg cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc
     9601 ctaaaatgtg gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc
     9661 tgttttctac agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg
     9721 aatttgttgt taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc
     9781 atgaaaaatt aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg
     9841 ctggatttga ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg
     9901 atgataaatt tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca
     9961 aactgaaagc gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc
    10021 catcaattga tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa
    10081 tagaaatcgt ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag
    10141 aatcatataa ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta
    10201 ttattgaaga tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt
    10261 ggtatacaaa taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa
    10321 aagagccagt aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac
    10381 gagaagaaat aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat
    10441 tttaaggtat actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta
    10501 tttgttacaa tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt
    10561 actgagtatt taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta
    10621 agtaggctag aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg
    10681 gtatttgtga atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa
    10741 gtaaagcaag ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat
    10801 cgtgagttct attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa
    10861 caagctgtta ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac
    10921 tatgcattta cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca
    10981 ggaccgctga agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg
    11041 atggggaatt atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta
    11101 gctatattga atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg
    11161 tttcaccgaa tagttaaaac attaaatgag tattatgacg attataatga ttttga
//
LOCUS       DAHUWM010000092        11085 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHUWM010000092 DAHUWM010000000
VERSION     DAHUWM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.093"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            47..1306
                     /gene="lysA"
                     /locus_tag="NK788_001660"
     CDS             47..1306
                     /gene="lysA"
                     /locus_tag="NK788_001660"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCH6769991.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1293..2093
                     /gene="dapF"
                     /locus_tag="NK788_001661"
     CDS             1293..2093
                     /gene="dapF"
                     /locus_tag="NK788_001661"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCH6769992.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2096..2977
                     /locus_tag="NK788_001662"
     CDS             2096..2977
                     /locus_tag="NK788_001662"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCH6769993.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2974..3690
                     /locus_tag="NK788_001663"
     CDS             2974..3690
                     /locus_tag="NK788_001663"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCH6769994.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3696..4391
                     /gene="dapD"
                     /locus_tag="NK788_001664"
     CDS             3696..4391
                     /gene="dapD"
                     /locus_tag="NK788_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCH6769995.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4407..5408
                     /locus_tag="NK788_001665"
     CDS             4407..5408
                     /locus_tag="NK788_001665"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCH6769996.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5405..6766
                     /locus_tag="NK788_001666"
     CDS             5405..6766
                     /locus_tag="NK788_001666"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCH6769997.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6726..7877
                     /locus_tag="NK788_001667"
     CDS             6726..7877
                     /locus_tag="NK788_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCH6769998.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7852..9021
                     /locus_tag="NK788_001668"
     CDS             7852..9021
                     /locus_tag="NK788_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6769999.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9196..10575
                     /locus_tag="NK788_001669"
     CDS             9196..10575
                     /locus_tag="NK788_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6770000.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10856..11085
                     /locus_tag="NK788_001670"
                     /pseudo
     CDS             10856..11085
                     /locus_tag="NK788_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3684 a         1941 c         2494 g         2966 t
ORIGIN      
        1 gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga cagaattgat
       61 aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc tgtatgtgta
      121 tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg tatcgaaacg
      181 ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga tgctgcattt
      241 agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct ataccgcttt
      301 gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga cgccagagga
      361 actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt tgatggaagt
      421 tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atctgtatca tgcttcggtt
      481 gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata ttgattcaaa
      541 attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga taaaagaaag
      601 cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt ttgatgttac
      661 tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg accaacctct
      721 ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc agccgatccc
      781 agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg aaaaacgaaa
      841 actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag aagctggtac
      901 aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt actttgtaga
      961 tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg attgtgattt
     1021 agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa aaatgtgtga
     1081 atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg gtgatctttt
     1141 agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca atcgagcgac
     1201 acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc gcagacaagc
     1261 attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat aatggctgcg
     1321 gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa ttagccgctt
     1381 ctttatgcac aaaagaaaaa tatgatacag acggattgat cgccgtaaaa atcgatccgt
     1441 tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga aatggcattc
     1501 gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa ttcaacgtag
     1561 agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt tattgcagcg
     1621 tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa acttatccaa
     1681 taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt ttatttttag
     1741 gaacgattca tacagttgtt tttgttgaaa atgcgcgtga ggaagtctgg agaaaaagag
     1801 gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac tttgtagaag
     1861 tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt tggacacttg
     1921 cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg aaaatttcag
     1981 cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt gaggatgcga
     2041 tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa tgaagatgat
     2101 aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag tagattatgc
     2161 tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat tattagtttt
     2221 aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga tccttcaact
     2281 gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa cgaataatac
     2341 gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg cacttttggt
     2401 catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt ttacagcaat
     2461 tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa caggcatgtc
     2521 gattccaatc catgtattag taaatttagc agaacatcct aatatcattg gtttgaaaga
     2581 agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag aagaattctt
     2641 cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag gaagcggtgt
     2701 aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa aagatacgca
     2761 ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac tgatccatgc
     2821 cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat tagacttacc
     2881 ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga aacaattagc
     2941 tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat tggcagtgga
     3001 cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt ccttctttta
     3061 cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga actgcttatt
     3121 gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc atatcaactt
     3181 ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat aaaagaacat
     3241 gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat ggtgatgaat
     3301 caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact gattgaaaaa
     3361 catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct tcaaacaatc
     3421 caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag agaagaaaac
     3481 caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga tgtattgttt
     3541 gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg aatctttgct
     3601 gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta ttacaaatta
     3661 gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa atcatccgct
     3721 acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta accgagccaa
     3781 tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt tttggtgact
     3841 atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa atcgaaatcg
     3901 aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt aatgctcgta
     3961 tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc gtgatcatga
     4021 tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt gatatgggtg
     4081 ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca ggagctgtct
     4141 tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac ggcgtactgg
     4201 taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca gtcgtggcag
     4261 caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga atacctgcac
     4321 gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt gctgcgttac
     4381 gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt gtgggggcaa
     4441 ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt ccagtaggac
     4501 gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc aagaaaaaga
     4561 aacacactgt acaggaattg acagaaactt catttgaagg atatgattta gcgctatttt
     4621 ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag ggactatttg
     4681 tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc gttccagagg
     4741 tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt tcaaccattc
     4801 aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga gtcaattatt
     4861 cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt catcgtacgc
     4921 aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg attccggaaa
     4981 tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata aatgagaccc
     5041 gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt gtgccaatcg
     5101 aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg acggaagaaa
     5161 ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta gcagcacata
     5221 aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg aggatcagaa
     5281 aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac atacgcaaag
     5341 gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata gcggaggtgg
     5401 caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt ctcatgaaga
     5461 gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag tgatcgttgt
     5521 ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac tttatttgat
     5581 ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga tttgtgatcg
     5641 tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg aactagcacg
     5701 catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa gtagaggtga
     5761 atatctgact gctcaactga tggctgagta tcttggttac cagtttattg acgcaaaaga
     5821 cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac aggaaatcac
     5881 aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg cgaaccctgc
     5941 aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga tactggcgaa
     6001 gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca tgatggctga
     6061 tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag aactgagaga
     6121 gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag ttcaagaggc
     6181 tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga caaaaatatc
     6241 gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca aggattttct
     6301 ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct ggaaagcaat
     6361 gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga tcgacaatat
     6421 cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca cgaaagaatt
     6481 aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt tgatctctgt
     6541 cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat cgatattgaa
     6601 caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga atctgatcat
     6661 cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag ggatggtgaa
     6721 tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc gggtttcatg
     6781 aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcggggcc gttatccatg
     6841 aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa acgattgctt
     6901 ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct tttgcttcta
     6961 agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg cttttagggt
     7021 tgactgactt tataactaaa cacattgagg atcttattta taatgtagta ctgatctatc
     7081 agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga ttgttggata
     7141 aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct aagggagaag
     7201 ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt atcattaaag
     7261 gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag gcagctgtac
     7321 gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg aatcatctaa
     7381 tgaaatttgg ccaaatccaa ggtggaacga tccgcaatgt tttagctgga gaagttattg
     7441 tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag aggggattag
     7501 accatttagc agaacagttt cactctacag ctactatttc ttttcgctat aatgacggct
     7561 atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca aacaaactac
     7621 atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg caacaatatc
     7681 catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat ccgacgttcg
     7741 attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt ctttttacag
     7801 aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat aatgatgaat
     7861 ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat acgacaattc
     7921 gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga accggatttt
     7981 ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga cttttctcac
     8041 tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt tcagcaacag
     8101 cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg tgcaacggaa
     8161 gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct tattccagcg
     8221 cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt gatcccgata
     8281 gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc atttgaacaa
     8341 tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac tgggacgaca
     8401 ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc ggtatttgtt
     8461 ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc catagctact
     8521 tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc gatgactggt
     8581 tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat taaagtccat
     8641 caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga agcgctaata
     8701 aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag agactatctg
     8761 gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc attttacttg
     8821 ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt agaattagcg
     8881 gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg agaaggttat
     8941 atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa acggatcaaa
     9001 gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt taattttgcg
     9061 gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg cgtttctttt
     9121 gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa aagaatattc
     9181 aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc tcatggaggg
     9241 catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat cacgctattt
     9301 gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt agaagacaag
     9361 gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca atccaatgtt
     9421 catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac agtaacggtt
     9481 actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat tttgagttct
     9541 ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt ctacttgatg
     9601 cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc tattaaaaaa
     9661 atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag ccgaaaagct
     9721 gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta tttagatgct
     9781 gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt tttgacaaat
     9841 gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac aagtgaaaaa
     9901 gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac agaatggtta
     9961 aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata cttgcaaacg
    10021 aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat cccagcacgc
    10081 aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta tgtagtaaaa
    10141 catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc ctctgcatta
    10201 agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc agcaaaagca
    10261 ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata tgtaccagaa
    10321 gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac ccatcaattg
    10381 ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt attggcacta
    10441 gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt cttccagcca
    10501 ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt aggagaagaa
    10561 ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag ctgccttgca
    10621 tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt ggctggactt
    10681 attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac tgtgacacga
    10741 tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg tcggtatttt
    10801 ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat ttggatggca
    10861 aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaaattcg
    10921 ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt
    10981 ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa aagtaacggc
    11041 atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DAHUWM010000093        10564 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHUWM010000093 DAHUWM010000000
VERSION     DAHUWM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.094"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            147..416
                     /gene="rpsN"
                     /locus_tag="NK788_001671"
     CDS             147..416
                     /gene="rpsN"
                     /locus_tag="NK788_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCH6770001.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            431..610
                     /gene="rpmF"
                     /locus_tag="NK788_001672"
     CDS             431..610
                     /gene="rpmF"
                     /locus_tag="NK788_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCH6770002.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            642..1019
                     /locus_tag="NK788_001673"
     CDS             642..1019
                     /locus_tag="NK788_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770003.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1040..1189
                     /gene="rpmG"
                     /locus_tag="NK788_001674"
     CDS             1040..1189
                     /gene="rpmG"
                     /locus_tag="NK788_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCH6770004.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1213..2196
                     /locus_tag="NK788_001675"
     CDS             1213..2196
                     /locus_tag="NK788_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCH6770005.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2226..2348
                     /locus_tag="NK788_001676"
     CDS             2226..2348
                     /locus_tag="NK788_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCH6770006.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2366..3889
                     /locus_tag="NK788_001677"
     CDS             2366..3889
                     /locus_tag="NK788_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCH6770007.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3913..4152
                     /locus_tag="NK788_001678"
     CDS             3913..4152
                     /locus_tag="NK788_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770008.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4345..4806)
                     /locus_tag="NK788_001679"
     CDS             complement(4345..4806)
                     /locus_tag="NK788_001679"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6770009.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5001..5219)
                     /locus_tag="NK788_001680"
     CDS             complement(5001..5219)
                     /locus_tag="NK788_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770010.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5337..6953
                     /locus_tag="NK788_001681"
     CDS             5337..6953
                     /locus_tag="NK788_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCH6770011.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7194..8534)
                     /locus_tag="NK788_001682"
                     /pseudo
     CDS             complement(7194..8534)
                     /locus_tag="NK788_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8810..8965)
                     /locus_tag="NK788_001683"
     CDS             complement(8810..8965)
                     /locus_tag="NK788_001683"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCH6770012.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9261..9593)
                     /locus_tag="NK788_001684"
     CDS             complement(9261..9593)
                     /locus_tag="NK788_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6770013.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10107..10298)
                     /locus_tag="NK788_001685"
     CDS             complement(10107..10298)
                     /locus_tag="NK788_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770014.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10392..>10564)
                     /locus_tag="NK788_001686"
     CDS             complement(10392..>10564)
                     /locus_tag="NK788_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCH6770015.1"
                     /translation="LAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATNLK
                     HRLYKKIQKMLV"
BASE COUNT         3506 a         1682 c         2060 g         3316 t
ORIGIN      
        1 tttcaaatag ttatctattc atatgagttt tatttcatgt tatttattgt tgacaagagc
       61 aactcttgtt ggtaaagtat cagtgtcaaa acgtaacgat tacgatttga ggtgtctaaa
      121 tcaaaataaa aggagtataa aggtatatgg caaaaaagtc gaaaattgct aaagcaaaga
      181 aacaaatggc gatgattgaa aagtatgcag ataaacgtca ggaattaaaa gccgcaggag
      241 atcgcaccgc tctcgctaaa ttgccaagag attcaaatcc gaatcgtctg aggcttcgtg
      301 atcagacgga tggtcgtcca agagggtata tgcgaaagtt cggtatgtcc cgtattaagt
      361 ttagagaact tgcccatcaa gggttgattc ctggagtaaa aaaagcaagt tggtaaaagg
      421 aggaaatatc atggcagtac cagcaagaaa aacatctaag gcgaagaaac ggttaagaag
      481 aactcatcaa actgtagtaa aaccagaaat ttcatttgac gaagcgaatg gtgattatag
      541 acgtagccat catgtatctt taaaagggta ctataatgga aagaaagtaa ttaaaggagc
      601 ttcaaaatag tttaatatat aacttttagg agggaatcaa aatggaaatt atttacccac
      661 ctttagttga agaaggatta aagtattatc ttgaaacgac ccagcaatcg ctagataaat
      721 gcacttttta tcgttcgatg gtagagagag gaattattac tgaaacgggt ttaccgaccc
      781 aacaggcaat tgaaaatgga ttagtcaaag attactatga ggaccaaggc ttatcatttg
      841 atgatttttt aagaatctat ccaatttttg aagagtatga cgaggagtta tttcaatgta
      901 tcgatggtta ttgggagata cctgttgata tgaaagaaaa tttagtttca caattggaat
      961 ctggcgagtt aacttttgaa gatgcacaac aaattcaagc atatcttgaa gatcgataag
     1021 tattgaagga ggaagattta tgcgtcaaaa tattattttg gaatgtgtcg aaactggaga
     1081 acgtctgtat ctcaccagca aaaataaacg gaataaccct gaacggttag aattaaaaaa
     1141 gtattcgcca aaattgcgca gacgggcaat ttttaaagaa gtgaaatagc taagtggtaa
     1201 aaaggtgaca aaatgggcat accagttaca atcgtttctg ggtttttagg ggctggaaaa
     1261 acaacgctaa tcaatcaagc cttgcaagtc tcaccctacc ccagggaaga aattattata
     1321 attgaaaatg agtttggtga agttgggatt gatcacaaat tattagttca tagcgaggaa
     1381 cgggtgttac aactaaataa tggatgtatg tgttgcagtc ttcgcagtga tttattagca
     1441 atattgattt ctttactgga aacaatagaa gaacatcaac aaccaatttc acaaatcatc
     1501 attgaaacta caggtatagc agatccccaa ccaatcattc aaacactttt aacagcgcct
     1561 caactaagag gaagagtata tatcgatagt ttattgactg ttattgatag tgacagtttt
     1621 gaacttattg aaagagaacc acaagccctt aagcaacttg tcatggcaga tcgcattttt
     1681 gtgtctagaa aacaaaactt gaatttatct gcactaaaaa aattacagag agaaatcaaa
     1741 agtattaacc ctttatccga tattcgaatg ttcacacata gtgaaacgat tcaaaaaaag
     1801 gatttttata atttgaaaaa atttaatcat cccttagttt ttgaaagtga ttcatcagaa
     1861 aatcactata atggcaaata ctatcatcaa caccatcaag tacatgagtt taaatcaatt
     1921 ctcttaactt cagatcaaga cataaaaaaa gattttttac tccaatggat tgactggctg
     1981 attttaacca accaagggag cctgtatcgt tttaaaggat tgattgcttt acaagagcag
     2041 gattttatag ttgccatgca agaggtaaac gaacaggtta attttcaata caccactcga
     2101 caaaaagacc aaaccactat tattctaatc ggaaaaatgt tggatgaaga aaggatcaaa
     2161 cagagttttg ataaattact ggcggaaagc aaataatgat aagtagtgat tgggggtaat
     2221 ttctaatgga gaaaacggat atatctagtg catatcgacg acttaaaagc ccgaatatta
     2281 aaactagaaa aagggcatta aaaattatca aagaacataa acgaaataaa atgaagaaat
     2341 tagcataatt aaggaaggaa aaagaatgaa gaaaatcagt gtaggactta ttgggatagc
     2401 agctctagga ttattgggag cttgctccag cactaatgat gcaaaagtat cgaatgataa
     2461 agatggaaaa ttagaaattg taacaacatt ctatccaatg tatgatttca ccaaaaacat
     2521 cgtcggcgat gaagcgaacg tggatttaat ggttccagcg ggatcagaac ctcatgacta
     2581 tgagccctca gctaaggata tggcaaaagc tcatgatgct gatgtttttg tatatcataa
     2641 cgaaaacatg gaatcatggg taccaaaagc taaagaaagt tggaagaaag ctgggcctaa
     2701 tgtagtagaa ggaacaaaag acatgatttt attgccaggt agtgaagaag aagatcatga
     2761 tcacggagaa gaagatcacc atcatgaact agatcctcat acgtgggttt cacccaaaat
     2821 ggcaattaag gaagtttcaa atatcaagga tcagttagtt aaattgtatc cgaaaaaagc
     2881 aaaagtattt gagacaaatg ctgaaaagta tttgactaaa ctgaaacggt tggatgctga
     2941 ctataccact agtcttaaag aagcgaaaca aaaaagtttt gtgacgcaac acgcagcctt
     3001 tggatattta gctttggatt atggattgaa acaagttcct attgctggac taagtcctga
     3061 agaagagcca tcttctggtc gattagctga attaaaagag tatgttaaaa aaaataagat
     3121 caattatatt tactttgaaa agaatgctaa tgataaaatt gcaaaaactt tggccaacga
     3181 agctgggata aaattggaag tactgaatcc tcttgaaagt cttacaaaag aacaaatgga
     3241 taatggtgaa gattatgttt cagtaatgga agacaatcta aaagcgttag aaaaaacaac
     3301 tatggttgct ggtgaagagg tagttccaga aaaagaagcg aaggatgaaa agacagttgc
     3361 taatgggtac tttaaagatg ccgatgtgaa ggatcgcgaa ttgagtgatt atacaggcga
     3421 atggcaatct gtttatccgt tgttaaaaga tggaattttg gatgaagttt tcgattacaa
     3481 agcaaaaatc aataaagaca tgacagctgc cgagtacaaa gattattata ctactggcta
     3541 taagacagat attgatacta tcaacattaa agacaacacc attgattttg tagtcaacgg
     3601 tgaacatcat caatacacat acaaatatgt tggatacaaa attttgaatt atgaaaaagg
     3661 aaaccgtggt gttcgtttta attttgaaac tgatgatgca ggtgctggtc gttttaaata
     3721 tattcaattt agtgatcatg gaattgcacc aagtaaggca gagcatttcc atattttctt
     3781 tggtggagaa agtcaagaaa aactctataa tgaattgcat aattggccta ctttctatcc
     3841 agcttcattg tctaaacatg aaattgctca agaaatgatg gctcattaaa attcaatttt
     3901 tggaggaaat acatggtaag aaaagtatta ttgtctatgg attccaagaa tattgatgct
     3961 tatcggcgtt atcgatttgc aaagcaaatg agattaaatg ggagtacatt agctgatatt
     4021 gctgcaactt tacatgttag tattgacaca gttcatagag tattaaaacg ggatcctgat
     4081 tgttgtttta tcaaggtcaa tactggaaga agtagacgtt tagcaaaacg gcataatagc
     4141 ttggccaatt aattgataat accagagtag atcatttttc ccttatttgg atttgatctg
     4201 ctttttatta tgtcttgaat tgtcaaatca agcgcaacac tttatcaaag aagacttcat
     4261 aaggtgttct ccaatctaaa cactttctag gtctgttatt cagtttttca atcatctgat
     4321 ttatcgttga gttatctacc gtagctatct ctttcgattt tggcaagtat tctcgaatga
     4381 gtctattcgt attttaattt gttcattgtt gccaagggca tgaggatctg gaaaataaaa
     4441 gggcatatta ttcaatgctt ctgtaactgc gaaatgattg gagaattctt ttcctctatc
     4501 tggagtgatc gttttgaccg cctcatttgg aatactgctt aaaagttcaa tcattttctc
     4561 gctcacataa gcggctttct ttctggggac cttctttcct gctaaaagga agcatgattt
     4621 tctgtctgtc aaagtcacta aacaagtacc accagttttg ccagctacaa tatcggcttt
     4681 ccaatgacca aaatcacttc tatcatctgc ggattttgga cggccatgga tcgaatgagt
     4741 aaggcaaatc tttccacgag actcgacatg attttttgta tgtcttgttt ttcctcgatg
     4801 gtgcaatgaa cggatcaggc ctttattgcc gtgtgagagg ggttctgtat cgaaaagatc
     4861 attgcaaatt gcacgttaga tcgtgataaa actaattgaa aagtccatat gttcatgctt
     4921 caaacgattc gagatttcct ctggcgacca ctgaaaatcg acaaataaat gacgaacaat
     4981 tgaccaaact cacggatcat ttagaatgtg ataacgtccg catttttttc ttcgataagc
     5041 ataatttttt tccgctttgg aaggtgaata gtttttttct tgtttcttct cagttcatgt
     5101 aaaatagtaa acgggctatg ctgaatgtcg ttgccaatta tactaataga aaatccttct
     5161 tcgtgaagca gaaagattct ttctcgttcg agtatagtaa gatgacgata ctggctcata
     5221 attattttcc ttctaggagt gttctcgaca aacttatact agttggatat cgattatgac
     5281 tcatttttat gtgttgcact taaagtgtaa attcaaggta tagaaagagg ttccctatga
     5341 aaaaaatacc tattacaatc ttaactggct atcttggttc aggaaaaacg atacttctaa
     5401 atcatattct gtacggtgat cacggaaagg aaattgctgt cattgtaaat gacatagggg
     5461 aagtaaatgt tgatggggta ttagtcgaca agaaaggttt taaaagaact ggagaaaaac
     5521 ttgtttcgat gccgaatgga tgcatttgtt gtacattacg cgaagatttg attgttgaac
     5581 tagatcaatt agtgcaattt tctgacttga atcaaattgt tattgaagct tcaggaatca
     5641 gcaaaccaat tccaattatt caagcaatta tattagctaa aaacccttta gacattgatt
     5701 tgacccaacg attagagatt ggtgccatta ttatagtagt ggatgtaaaa cgtttttggg
     5761 ataatttcga aacgggtgaa aagattcagg aaagaaaagc tgaaggtaat gtagttgaag
     5821 aagaacagga tattcgtggc ttgttaattg atcaattcga gtgttgtaac atattacttt
     5881 taaataatag tgacttagtg tcaaccaaga cgatttacaa aattaacggt ctaattgcta
     5941 agttacagtc caatgccaaa ataattagga caattaaagg tgaagtagac ttatcgaaaa
     6001 ttctaaatac aaatcttttt aatatagatg agacttccta ttcagtgggc tggttcaaag
     6061 agttagagct tggctatcaa aatcatattc ctgaaacaga agaatatggt atctcaagtt
     6121 ttatctatcg aaatcggtta ctccttgacg cacaaaaatt tgcccatctt acggataaaa
     6181 attttcctgg caatattaca agagcaaaag ggtttctttg gtttgcccaa catagtgatt
     6241 tttcgacgat ctttgaaaca tcgggtagct ataaaagtat tgaatcatta aattattggg
     6301 tagctagttt acctaaaatt gagtgtcaaa tgattataca aaaaaatccc gaattgaagg
     6361 aagcctggca tccgttgttt ggtgaacgca ttaataaact agtttttatt ggtatgaaga
     6421 tggatcaaca atgtattaag gaacaacttg agggttgttt aactgattct cttaatgcag
     6481 ataatttccg aaaaatttca ccatttccat ggaaaaagtc taatatgttt agccagatat
     6541 cgttaatgaa atttttaaga aaagctggtg tcaagatagg cgatgtcata attattggca
     6601 ttttgattat tacctctttt attcccttat ttgtattctc tatagaaaag gagaatgaaa
     6661 caactgaaga tgctatcaaa tatgccatcg ttcgaattga tggcaaggaa atggatcgct
     6721 tcgatttgga taagatcaat cataaactcg tgaattatca tcctgcgaag ggacaatata
     6781 atattgttga gattaaagat ggaagaattc gagttaaaga agataatagt cctgatcaaa
     6841 tcgcggttag aactggatgg atctcaaaac ccggtcaaac gagtatttgt ttaccccata
     6901 agttagttat tagtattgag aaaaaagaaa gcacagatta ctacatttac taatctggaa
     6961 atagatgagg aggtttttaa taagaaaaag atattattat taataatagc aggggcttta
     7021 ttaagtgtgt taagcgcttg tggacaagaa aaagaagcgg tgacaacagg cacaagtatc
     7081 tttaacggga cctgcaatgg tttagttaac agtcggttag attacttatt ttggctcttc
     7141 gtcaatgagt gttggtgaag aaattcaata gaataatcga gatactcaca cggctagaca
     7201 gctatcttgt ctagccgtgt tgttgttctg gctttagtca gttgggtagt attcttagtt
     7261 ttcttaaaat attttcctcg atataaaaag attcgttctc ggtagttttg aaagtttcta
     7321 tagccatagg caattcgttt tagtactttg atatggttat tcaaacattc tatagggccg
     7381 ttggaatagg gaacgcgcaa cgctcgtttg atttctgttt ggtatttctt gaaggtcgtg
     7441 aacactggtt ggtagtgctc tggcaactcc ttgtagtctt cttttaggag cgcgacgaat
     7501 tcaggaacat cttgtcttct gataacggaa agaaaatcct ggtagacttc gtagcctcgc
     7561 tttaaggaat catcataaga taatagccga tcgactatct ctgcttctgt cagatgatct
     7621 cgaaatgacg gacgccagta gcgatgctga cctttcaatt cccacgcatt cttttgaagt
     7681 agtttccagt atttctttaa gtgcttcgac cgacgatccc cttttttaaa ggtcttcatt
     7741 acttgaattc ggtggttggt aaaagcccga ccaatgtgtt ggctaatatg aaagcgatcg
     7801 gcgacgattc gggcattcgg aaaacactct ttcaccaatg tgcgataggg tgtatagaaa
     7861 tctgaaacca ctagacgcac ttgttcacgg acttttatag ggtagcgcaa gaaatagtcg
     7921 cgtaaaggat tgagttgccg attttccaca atatcaatca gttgatgggt cttgccgtcc
     7981 atcatgacaa agctcatggc tcccgagaca tttttcactg atttgaattc atcgaaacac
     8041 accacttctg gtaacagctg gctcaaagaa atggttttag gccgaaggct gcgaagtatt
     8101 cgaatgacag tggtcggaga aatgtgcttc atacgtgcga tcaaagacat agaaagaggt
     8161 tcgcttaata gctcaagaat ggcttgtttg actctgcgag caatggagca gcgacgatca
     8221 ctaactgaat cttctgctaa aaaagtcttt ccacagtgtt tacatttgaa gcgctgtttg
     8281 ttaatccgca gaatggtctt gtagttactg acatcattca ataagatgga tacttttttc
     8341 cagccctatt taatgatcgt ttgttcattt tgatagtggc gatgaaagca ttcctccggt
     8401 tgataggtca atgtgccatc aaaaacaaaa catgtttcgt cattgattcg ctctttgcga
     8461 aaggcatctt catgaaagat aagatttagg tctagaatat ctagtaactc tttggtaaac
     8521 tctgaatagg acatcttgaa acactccttt gaatggtttt cgtcgacttt attctaaagg
     8581 attttaaggt gtctgtttgc gtttattaga aaaaagaaaa agtattggca acagaaggcg
     8641 tcaccaacac tagatattat agatccctta ttttcttcgc ttcaaattgt caaggagtca
     8701 tcataaattt tgtatggatt cttttgtttg ggtagtagtc ttctaaataa tcttggatta
     8761 acaccttcat ctctatttta cttttatatt tgttggagtc gattaattct catttgattg
     8821 atcgatgaaa cgcttcaatt actgcattgt caacaggagt cccagtttgg ctcactgaat
     8881 gtgtgatgtg gtggtcgttc aacagctctt gataaataaa agaccgatgc tggctacctc
     8941 tatcggaatg aattaccgta ggcaattctc cttaataaat ttgtaatgga cggacgacaa
     9001 tctctttttt catgtgtgaa tctaccatat agtaacgtag aatacgagtt gctaagtcaa
     9061 tataggtgga caaatagagg cgaccgtcat tacatggaat gtaagtaata tccatcaccc
     9121 aaacagcatc aatttggttc tgttcaaatt atcgattcaa cattttttta ccatttcttt
     9181 gattgttttt tctctaccat gagaagattt gcgtcgatga aatcctcttg tgcataaatt
     9241 catttcacgc ataagaacag ctacaacacg tttattgatt tcgattcctt catcgtaaag
     9301 tttttgagtg atccctggag atccgtatcg atctttgtgt tcgtagaaca ctcgtcttcc
     9361 tcttttagtc gtttattttc ctgttcaaga atgtagaaat ctgctttatc aggtaatctt
     9421 tttccttgtc cagcaaaagc atttttcccg tatcgacgat attctgaaac ccatcgggaa
     9481 attgttggac gagagatatt gtattcttca gcaattttct tggtagttac ttttttctca
     9541 aggatatcct gtgaaatctt tagtttaaat ttacgatcat atgtttttct caatagagtt
     9601 cattccttcc ggtaacgtaa ctgatagtca agaaaagtgt atcatagccc aacttagtat
     9661 cagggtttgt caactaaact gtggaagttg atagataaga gtttttaaca accaaaattc
     9721 tcttgactat ttttgaaatc agacaaattt tgattggaca catagttgaa taaatctacc
     9781 gtcgttacgg aaggtaaatc gcatgctttt caattctaga ggagaagtcc tcgttgaagt
     9841 accatagccc agttctggat tcgacctcct gcccacttca tacgtaaata aagtaatttt
     9901 aggagtgaga ctgtgacaaa agttattttt aagctaaaaa cgaattaaaa aacgttgata
     9961 attaaaatca ttcgttttag attctatgaa tcattataga acgctgtaaa atcgatactt
    10021 tataattata aagaagatag tttcaaattt attgttcgaa atttgaaact atctttttac
    10081 ttatgtcaca gactccctta gttgagttat tctttaattt cgaatttcga gatagctaga
    10141 tatattgcaa gcatacattc ttgataacaa tcttgatcat attttccagt tctccaggat
    10201 tgcttatgaa tcaaagagct aaatcttttg agaattgcct caacagcttc ttcatttcca
    10261 gcctttgcta aaatgaaatc ttcttttaaa gaattcataa atctaccttc ttcccatgga
    10321 cttataatct atttctttgg gaaggaggag aacgagcaaa gaagcataca aaattagtta
    10381 ttatgcagat attaaaccag cattttttga atttttttgt aaagtcgatg ttttaaattt
    10441 gtcgcaccct gtcttgtaat ccccagaacc tttccaattt ctttatcagt tttatcaaag
    10501 ataaattttt cgataagaaa aatttgttca ggttctttca acaaaggaag gatttttgct
    10561 aatt
//
LOCUS       DAHUWM010000094        10532 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHUWM010000094 DAHUWM010000000
VERSION     DAHUWM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.095"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(25..162)
                     /locus_tag="NK788_001687"
     CDS             complement(25..162)
                     /locus_tag="NK788_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCH6770016.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="NK788_001688"
     CDS             complement(159..1343)
                     /locus_tag="NK788_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCH6770017.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="NK788_001689"
     CDS             complement(1599..1853)
                     /locus_tag="NK788_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770018.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="NK788_001690"
     CDS             complement(1878..3020)
                     /locus_tag="NK788_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCH6770019.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="NK788_001691"
     CDS             complement(3080..4432)
                     /locus_tag="NK788_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCH6770020.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="NK788_001692"
     CDS             complement(4503..4847)
                     /locus_tag="NK788_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCH6770021.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="NK788_001693"
     CDS             complement(4857..5231)
                     /locus_tag="NK788_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6770022.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="NK788_001694"
     CDS             complement(5244..5558)
                     /locus_tag="NK788_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6770023.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="NK788_001695"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="NK788_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="NK788_001696"
     CDS             complement(8976..9638)
                     /locus_tag="NK788_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770024.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="NK788_001697"
     CDS             10255..10485
                     /locus_tag="NK788_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770025.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DAHUWM010000095        10489 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHUWM010000095 DAHUWM010000000
VERSION     DAHUWM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.096"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            13..636
                     /locus_tag="NK788_001698"
     CDS             13..636
                     /locus_tag="NK788_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770026.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /locus_tag="NK788_001699"
     CDS             1086..2135
                     /locus_tag="NK788_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6770027.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="NK788_001700"
     CDS             2132..3013
                     /locus_tag="NK788_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770028.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="NK788_001701"
     CDS             3016..3786
                     /locus_tag="NK788_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6770029.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="NK788_001702"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="NK788_001702"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCH6770030.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="NK788_001703"
     CDS             5097..6260
                     /locus_tag="NK788_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCH6770031.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="NK788_001704"
     CDS             6377..6787
                     /locus_tag="NK788_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6770032.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="NK788_001705"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="NK788_001705"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCH6770033.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9360..9644
                     /locus_tag="NK788_001706"
     CDS             9360..9644
                     /locus_tag="NK788_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770034.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9804..10040
                     /locus_tag="NK788_001707"
     CDS             9804..10040
                     /locus_tag="NK788_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCH6770035.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10037..10435
                     /locus_tag="NK788_001708"
     CDS             10037..10435
                     /locus_tag="NK788_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCH6770036.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3650 a         1767 c         2224 g         2848 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa aagcaaaata
     9721 aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact atagaatgag
     9781 aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt agaaaatctg
     9841 gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt gtgcagacag
     9901 gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc aaagcacaag
     9961 aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa gacgcgttaa
    10021 aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa tcaacgcgaa
    10081 agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
    10141 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
    10201 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
    10261 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
    10321 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
    10381 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
    10441 ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgc
//
LOCUS       DAHUWM010000096        10259 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHUWM010000096 DAHUWM010000000
VERSION     DAHUWM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.097"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(53..1561)
                     /locus_tag="NK788_001709"
     CDS             complement(53..1561)
                     /locus_tag="NK788_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCH6770037.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1720..1911
                     /locus_tag="NK788_001710"
     CDS             1720..1911
                     /locus_tag="NK788_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770038.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2114..3031
                     /locus_tag="NK788_001711"
     CDS             2114..3031
                     /locus_tag="NK788_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCH6770039.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3024..4097
                     /locus_tag="NK788_001712"
     CDS             3024..4097
                     /locus_tag="NK788_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770040.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4245..4763
                     /locus_tag="NK788_001713"
     CDS             4245..4763
                     /locus_tag="NK788_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770041.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4744..5136
                     /locus_tag="NK788_001714"
     CDS             4744..5136
                     /locus_tag="NK788_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCH6770042.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5212..6147
                     /locus_tag="NK788_001715"
     CDS             5212..6147
                     /locus_tag="NK788_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCH6770043.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6280..7059
                     /locus_tag="NK788_001716"
     CDS             6280..7059
                     /locus_tag="NK788_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCH6770044.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7052..7369
                     /locus_tag="NK788_001717"
     CDS             7052..7369
                     /locus_tag="NK788_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCH6770045.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7613..9223
                     /locus_tag="NK788_001718"
     CDS             7613..9223
                     /locus_tag="NK788_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6770046.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9380..10228
                     /locus_tag="NK788_001719"
     CDS             9380..10228
                     /locus_tag="NK788_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6770047.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3224 a         1798 c         2071 g         3166 t
ORIGIN      
        1 actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt
       61 aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca
      121 atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt
      181 cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg attgataaag
      241 cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca
      301 atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg
      361 ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac
      421 gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg
      481 taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt
      541 cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt
      601 cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt
      661 tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca
      721 tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata atacgtttga
      781 ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa
      841 agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa
      901 tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac
      961 cagctgcatg tcctttttgg ctttcatgat acagttggtt caaggaagta gcgagctttc
     1021 cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat
     1081 tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt
     1141 ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg
     1201 caggctgtcc actatagcga gtgataacga cactgttgat taataagcca taaccacgta
     1261 attcatcgat taaccgcaag atatccatat cataagtaat cccataatca ccacggatct
     1321 tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt
     1381 tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct
     1441 tgtcgtcaac tagcttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt
     1501 ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca
     1561 ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg
     1621 aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt
     1681 attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag
     1741 aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa
     1801 gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt
     1861 gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga
     1921 taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa
     1981 ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac
     2041 aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga
     2101 ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga
     2161 ggagacgaat gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga
     2221 aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt
     2281 ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt
     2341 aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga
     2401 tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag
     2461 ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct
     2521 gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga
     2581 gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac
     2641 agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa
     2701 cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa
     2761 gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga
     2821 aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc
     2881 agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt
     2941 cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca
     3001 atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg
     3061 aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc
     3121 ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacac gaaaaagaaa
     3181 aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa
     3241 atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa
     3301 cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc
     3361 cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg
     3421 gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg
     3481 gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt
     3541 atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac
     3601 gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg
     3661 tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg
     3721 cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac
     3781 ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc
     3841 agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa
     3901 atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag
     3961 gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg
     4021 gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa
     4081 tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt
     4141 ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa
     4201 atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa
     4261 tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta
     4321 ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa
     4381 aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg
     4441 gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg
     4501 tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg
     4561 cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc
     4621 aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg
     4681 ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa
     4741 aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca
     4801 agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg
     4861 caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtgttc
     4921 ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat
     4981 aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg
     5041 atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat
     5101 ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg
     5161 cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat
     5221 tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct
     5281 atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc
     5341 cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat
     5401 aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca
     5461 atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa
     5521 aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta
     5581 tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg
     5641 aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg
     5701 attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca
     5761 atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt
     5821 ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt
     5881 gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc
     5941 agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca
     6001 aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa
     6061 aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa
     6121 tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga
     6181 aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga
     6241 gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg
     6301 ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga
     6361 ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta
     6421 tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa
     6481 gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta tacattatta
     6541 ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct
     6601 caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc
     6661 tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc
     6721 aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt
     6781 gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc
     6841 tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact
     6901 ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca
     6961 ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc
     7021 ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt
     7081 tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca
     7141 tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc
     7201 ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat
     7261 caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt
     7321 gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt
     7381 ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg
     7441 caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta
     7501 tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt
     7561 gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa
     7621 aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg
     7681 ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc
     7741 tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt
     7801 cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc
     7861 tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc
     7921 tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt
     7981 taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct
     8041 tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt
     8101 gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt
     8161 attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg
     8221 tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact
     8281 taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc
     8341 tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat
     8401 gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca
     8461 gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct
     8521 gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa
     8581 aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat
     8641 tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt
     8701 tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt
     8761 ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat
     8821 ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt
     8881 agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg
     8941 cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt
     9001 tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt
     9061 tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc
     9121 tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg
     9181 aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt
     9241 tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa
     9301 ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg
     9361 aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc
     9421 ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg
     9481 gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa
     9541 cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga
     9601 ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc
     9661 tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc
     9721 ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt
     9781 aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa
     9841 tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac
     9901 aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga
     9961 agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga
    10021 agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca
    10081 tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa
    10141 tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc
    10201 catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DAHUWM010000097        10146 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHUWM010000097 DAHUWM010000000
VERSION     DAHUWM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.098"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            343..1761
                     /gene="nhaC"
                     /locus_tag="NK788_001720"
     CDS             343..1761
                     /gene="nhaC"
                     /locus_tag="NK788_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCH6770048.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(2265..2708)
                     /locus_tag="NK788_001721"
     CDS             complement(2265..2708)
                     /locus_tag="NK788_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCH6770049.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2926..4266)
                     /locus_tag="NK788_001722"
     CDS             complement(2926..4266)
                     /locus_tag="NK788_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6770050.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            4535..5431
                     /locus_tag="NK788_001723"
     CDS             4535..5431
                     /locus_tag="NK788_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCH6770051.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(5581..6522)
                     /locus_tag="NK788_001724"
     CDS             complement(5581..6522)
                     /locus_tag="NK788_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCH6770052.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            6648..7769
                     /locus_tag="NK788_001725"
     CDS             6648..7769
                     /locus_tag="NK788_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCH6770053.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8027..9529
                     /gene="eat(A)"
                     /locus_tag="NK788_001726"
     CDS             8027..9529
                     /gene="eat(A)"
                     /locus_tag="NK788_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCH6770054.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            9557..10123
                     /locus_tag="NK788_001727"
     CDS             9557..10123
                     /locus_tag="NK788_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6770055.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         3299 a         1866 c         2029 g         2952 t
ORIGIN      
        1 tttctttttc attgacaaat gatcttatgg aaggtaaact tcgtacaagt caacttttca
       61 cgcagtagcg gttaggtgtt ttgttcagaa agtcggtggt cgctgtgaac cgatcaagcc
      121 taattgacga attacatgga aagtccatac atttgaataa aagtttgaaa gtgaaaaaca
      181 gatctgtttt taattaaggt ggtaccgcgg agtcattcgt ccttagttta aaacaggtct
      241 ttttattttt ataaaggagg aatcgcggat gaattgcggc ggagtaatcc gatatcttaa
      301 cttttttaga acataagaac aactaaaaaa ggagagatta tcatgggaaa aaaaggaaca
      361 ccaagttttt tagaagcaac acttgtatta atggttattg ttttaggcat tgcgattggc
      421 gttgtcggat taaaggtctc acctaatatc acgatcttag cagcgatcgg aatggtcatg
      481 ttgtatgcag tgataaaaag atacccgaca gattggctgc acgaaggaat catcaatggc
      541 atcaaaccag gaatcatccc aatctttatt tttattttag tcggttcatt gattgctgta
      601 tggatacaag cagggatcat tccaactcta atggtcattg ggttcaaatt gatcagtgtc
      661 cagtggtttg tcccttctgt atttgttgtg tgtgcaatcg taggtagcgc tgtagggagt
      721 gcctttactg ttatgtcaac gattgggatt gcatttttcg gtattgggac aacactaggc
      781 ttaaatccag cgctggttgt tggagctatt gtttcaggtg ccgtttttgg agataagatg
      841 tctccattat ccgaatcaac aaatctagca gcagctattg ttgatgccga tttgttcaaa
      901 cacatcaagc atatgatgtg gtcaactgtc ccggcatttg ttgtttcttt ctttttattc
      961 atgattttag gacacacaag taaagccgca agtctttcat ctattcgtga agtgactgat
     1021 gttctagaag caaactttac tatttcaatc tggtcactga ttcctttatt tttaatgtta
     1081 ctgtgtgcat ggaaaaaggt gccggcaatt ttaacgattt tgttgaatat cgctgtagca
     1141 gtaggaatga tttttattca aaacccacaa acttccttaa cgaagttggc tggggtcatc
     1201 gaatcagggt ttgtttcaaa tactggaaac caacaaatcg attcactgct tagccgcgga
     1261 ggaatcgaaa gtatgatgcc tactgtgtca ttgattattc taactttgtc attaggtgga
     1321 ttgttgatcg aatttggctt gatctcaacc gtgatggaac gagtatcaag aaagatgacg
     1381 aacacaccaa atttgatttt tacaacattg atgacaagta tcggagtaaa tatttttatt
     1441 ggtgaacaat tcctatctgt tattctgcct ggtaatgcat ttaaagaaac ttatgaaaaa
     1501 gcaggatttg atccaactgt attaggtaga acgctagaag atggtggaac agtaatcaac
     1561 tacttagtgc cttggggaat tgccggaagt ttcgtagcaa gtacatttgg tattcctaca
     1621 ctcacttact tgccattcgt atttttcagt ttgctttctc ctgtgttttc gatggtcagt
     1681 gccttcacag gttggggaat ctcaaaaaaa tttgtgaaac cgcaagaagc agtaagcaag
     1741 acagcgcaat tagaacaata aaaacataaa aagggattgt gacaagactt ttgtcacaat
     1801 cccttttacg aataaacggt attcaaaagc cagtttctcg gtattaagcc aaatttatac
     1861 aaaaatgtgg aaagccattt cacaaaattt ttcttaatac gcgaaactaa gcgacttttt
     1921 cacaacctct cgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg
     1981 caaaatatga ggaactattt ttccatttgt ctctctaata ctcaggtcag aacgctgatt
     2041 ttacaacctc ttaataaacg gtgttcaaaa gcttgcatct gcggtaataa gcctaaccaa
     2101 acgaaaaata ggagaagcta tttttgtttg gctgttctta ttacttgatg cagggcagct
     2161 ttttcacaac cccttattca cggcgttcca gaagtagctt cttcttattt cttgaagctg
     2221 ataacttctg gaacatcctc tttttttatt tggacggata atggtcattc catttctttg
     2281 atcatttttt tcattttgtc gatctcttct tcttgtgcct gaatgatttc ttccgctaat
     2341 ttttttactt caggatcttt taaatcagct cgtttacttg ccaggatagc gatagaatga
     2401 tgaggaatca tggccttcat ccaagctgtg tcgttgacag tagtttggct tcgtaccata
     2461 aacaaggaac cggcaaaaac aagaagacta gcggctaaga tcataacatt tatctttttg
     2521 ttttcataca ttttccacat gaacaacagc atgatgactg ccatgactgc acccatcatc
     2581 aacgccatga atactcgtgt acgactgaag aagatatgat cgagcgcata gacattcaag
     2641 tacatcatac caaacataac taccgttgaa acaccgatca tcaacaaaaa cttccaatac
     2701 tttttcattt aaaacaaact cctttttttc attcctttct tctatcatgt catgagcatg
     2761 gagaacaatc aattattttg cacagagggt ttttgaacac cgtttaccaa gaggtcatga
     2821 gactgtttat tcggaaaaag gagttgcaac aagacttttg tcacaactcc tttcttcaat
     2881 agctccatct attccacata aataaaataa aattttctat cgatttcatt cattcgtcaa
     2941 tacgaacgca tcattctttt tttgataact gaaataaatg aacaggtaaa gcaggccagt
     3001 caatacagtt accccaatgg agttcgtgaa caggatacca aggaaaatca atcctaagac
     3061 agctaatgca aaaaagctga ttcctttatc ccataatgtt ccttttctta gtgccagtac
     3121 aaaggatgcc gcactgataa tgatccagat caataatact gtatacgaaa gggaacctgc
     3181 cagatagcca aataatttgt caccgacaaa ataagaaaga gctacaccaa gataaagaac
     3241 actggtacaa aataaaacag cacgttgcgg tacttgatgc ttgtttaaaa cagctagttt
     3301 tctagaggaa ccttttcgat ccttcaaacg gaagaacaaa attcttgaag atgcatacac
     3361 acccgaattg atagatgaga acaaagcaag gacaatcaca aaattgacga tatccgctgc
     3421 aaatgggata ttcatttttt caaataccat cacaaaagga ctgacattcg tgcctgctaa
     3481 agtattccaa ggaaaaagaa tcaaaagcaa aaacaaagga atgatgtaaa aggaaatgat
     3541 tcttcccatg acacctttga ttgctttagg aatcgcacgt ttaggatcat ctgcttcgct
     3601 taccgtgata gcaatcagtt cagaaccgcc gtaagaatag ataactacaa gcaaagaact
     3661 gatgattccc ttcataccat gaggagtaaa cccaccatgg tcagtcaatt gctgaagggt
     3721 agggaaaacg cctgtgccca acatttgctg accgacaaga taagtagcaa aaatgatcag
     3781 taagataatg acactgattt tagcaaaagc cagccaatat tctgtttctg cgaataaccg
     3841 taccgaataa agattgatga tcgtagttaa aacagccaac ataaaaacaa atacccaggc
     3901 tggaatgtgc gggaaccaca actgcaaaaa ggaagcagct gcaacagctt cagcgatgat
     3961 attgataatc cacatagacc agtagaccca atcagtaaaa tcagcagaat gagcacctag
     4021 ataagggcgt atcaaaccag ataatccgtg atgcgtgtcg ccaccgctta aaacgatttt
     4081 tcccagacct ttcatcacga tatataaaat cagcccacca atcaaaaaag caatcaatac
     4141 agaagggccg gcgatcccaa tcgcttcgcc acttccttta aatagacctg caccgattgc
     4201 tccacctaaa gctaacatgg tgatatgtct ggagctaagt tgttttttca attttttatc
     4261 ttccataaat gacctctttc ttttaaattc acaatcaaaa tttatattaa gaataaatag
     4321 caatatttaa caatgactaa tgataacaaa ttttcagaca attacaacta ttcagaaaac
     4381 ttttaggcga attttaatag aagaatgatt tatttcatca tattttcata caaatgttaa
     4441 attttcatac aaatatagga aacgttttac tggcttttta ctatttaaac aggctatcat
     4501 aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg tactgctagt
     4561 tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg tttggtccgg
     4621 aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg caaattatca
     4681 gctgatcgtt tttttagatt atggatttga tatggagatg gctcgacgcg tgcgcccatt
     4741 taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac acgaggcgat
     4801 gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg attttttgga
     4861 agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata tgaatttttc
     4921 ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga gaaaagaacg
     4981 agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact atttcattct
     5041 tccacatcga gggaatgaac aacctggata tcttacttat gaagagtacc tgaaaaaaac
     5101 aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag tgactttgcg
     5161 gacatttgaa tccatgtatt ttcaaaagaa actagtgacg gataatcaag cgattgctca
     5221 ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt tagatgaatt
     5281 accggaattt ctgcatacac catttaagcc aatcctatca gagctattgg aattttatga
     5341 tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga
     5401 atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga gattatgtac
     5461 tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt
     5521 tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt
     5581 ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata
     5641 tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga tgtagtcgat
     5701 ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat
     5761 gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat ctagtatgat
     5821 tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc
     5881 cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa
     5941 tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc tatagatcgg
     6001 atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg
     6061 ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc
     6121 tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc
     6181 cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat
     6241 aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta atgctacgat
     6301 caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc
     6361 gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg
     6421 agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat
     6481 tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc tctcctttat
     6541 tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt tttgtttgaa
     6601 aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg gatattttgg
     6661 cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag gcagttctaa
     6721 atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa
     6781 atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt gatgggatgg
     6841 atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa ggaaaaaaca
     6901 ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa ccaacggatc
     6961 aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat gcactgcttc
     7021 aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat ggtggtctag
     7081 gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat cctttattat
     7141 ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta aaattgatta
     7201 tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg caatttggaa
     7261 gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca ggaaagattg
     7321 ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga agtggagcag
     7381 caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct ggtttcgatt
     7441 tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag
     7501 gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc
     7561 ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag
     7621 aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag
     7681 aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta
     7741 gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta
     7801 atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa
     7861 agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg
     7921 gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag
     7981 ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga
     8041 aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa
     8101 tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac
     8161 aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca
     8221 agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt
     8281 aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac
     8341 agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt
     8401 cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa
     8461 tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg
     8521 cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc
     8581 aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa
     8641 aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag
     8701 gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac
     8761 aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg
     8821 tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca
     8881 gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa
     8941 tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga
     9001 aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc
     9061 aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg
     9121 caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa
     9181 ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca
     9241 agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat
     9301 cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc
     9361 agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca
     9421 attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac
     9481 cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc
     9541 tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct
     9601 ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc
     9661 agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt
     9721 agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac
     9781 agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga
     9841 aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg
     9901 tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat
     9961 cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca
    10021 taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt
    10081 tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt tttgtgttat
    10141 ttaaat
//
LOCUS       DAHUWM010000098        10142 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHUWM010000098 DAHUWM010000000
VERSION     DAHUWM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.099"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            87..236
                     /locus_tag="NK788_001728"
     CDS             87..236
                     /locus_tag="NK788_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCH6770056.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            247..1761
                     /gene="dltA"
                     /locus_tag="NK788_001729"
     CDS             247..1761
                     /gene="dltA"
                     /locus_tag="NK788_001729"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCH6770057.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1758..2963
                     /gene="dltB"
                     /locus_tag="NK788_001730"
     CDS             1758..2963
                     /gene="dltB"
                     /locus_tag="NK788_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCH6770058.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3008..3241
                     /gene="dltC"
                     /locus_tag="NK788_001731"
     CDS             3008..3241
                     /gene="dltC"
                     /locus_tag="NK788_001731"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCH6770059.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3244..4512
                     /gene="dltD"
                     /locus_tag="NK788_001732"
     CDS             3244..4512
                     /gene="dltD"
                     /locus_tag="NK788_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCH6770060.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4653..5735
                     /locus_tag="NK788_001733"
     CDS             4653..5735
                     /locus_tag="NK788_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCH6770061.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5735..6538
                     /locus_tag="NK788_001734"
     CDS             5735..6538
                     /locus_tag="NK788_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCH6770062.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6608..7348
                     /locus_tag="NK788_001735"
     CDS             6608..7348
                     /locus_tag="NK788_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCH6770063.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7359..8363
                     /locus_tag="NK788_001736"
     CDS             7359..8363
                     /locus_tag="NK788_001736"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCH6770064.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8408..10105)
                     /locus_tag="NK788_001737"
     CDS             complement(8408..10105)
                     /locus_tag="NK788_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770065.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3243 a         1687 c         2064 g         3148 t
ORIGIN      
        1 aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt
       61 gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt
      121 attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt
      181 atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg
      241 aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct
      301 gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca
      361 gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt
      421 ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc
      481 tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct
      541 aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc
      601 ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat
      661 agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca
      721 aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac
      781 tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg
      841 gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca
      901 gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg
      961 gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat
     1021 gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa
     1081 agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca
     1141 actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta
     1201 ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt
     1261 tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg
     1321 aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat
     1381 acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat
     1441 cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc
     1501 caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag
     1561 cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact
     1621 aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc
     1681 cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca
     1741 gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt
     1801 tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa
     1861 gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta
     1921 attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat
     1981 ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta
     2041 ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag
     2101 catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca
     2161 tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc
     2221 tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc
     2281 agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata
     2341 tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc
     2401 ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata
     2461 tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg
     2521 gtacaagcta catgatggga tatcagacac caatcaattt caataaacct tttttatcat
     2581 ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt
     2641 atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg
     2701 taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga
     2761 cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat
     2821 ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct
     2881 tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt
     2941 tagataaact tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa
     3001 aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt
     3061 aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt
     3121 tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga
     3181 gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg
     3241 agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg
     3301 tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt
     3361 gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta
     3421 tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt
     3481 catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca
     3541 cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa
     3601 aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact
     3661 gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag
     3721 aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa
     3781 gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct
     3841 gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc
     3901 agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca
     3961 aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg
     4021 acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag
     4081 ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat
     4141 tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca
     4201 ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt
     4261 gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc
     4321 aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg
     4381 ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag
     4441 tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta
     4501 caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt
     4561 ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat
     4621 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
     4681 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
     4741 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
     4801 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
     4861 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
     4921 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
     4981 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
     5041 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
     5101 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
     5161 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
     5221 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
     5281 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
     5341 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
     5401 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
     5461 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
     5521 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
     5581 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
     5641 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
     5701 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
     5761 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
     5821 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
     5881 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
     5941 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
     6001 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
     6061 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
     6121 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
     6181 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
     6241 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
     6301 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
     6361 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
     6421 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
     6481 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
     6541 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
     6601 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
     6661 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
     6721 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
     6781 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
     6841 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
     6901 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
     6961 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
     7021 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
     7081 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
     7141 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
     7201 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
     7261 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
     7321 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
     7381 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
     7441 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
     7501 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
     7561 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
     7621 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
     7681 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
     7741 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
     7801 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
     7861 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
     7921 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
     7981 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
     8041 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
     8101 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
     8161 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
     8221 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
     8281 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
     8341 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
     8401 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
     8461 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
     8521 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
     8581 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
     8641 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
     8701 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
     8761 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
     8821 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
     8881 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
     8941 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
     9001 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
     9061 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
     9121 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
     9181 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
     9241 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
     9301 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
     9361 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
     9421 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
     9481 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
     9541 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
     9601 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
     9661 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
     9721 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
     9781 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
     9841 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
     9901 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
     9961 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
    10021 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa
    10081 cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa
    10141 ag
//
LOCUS       DAHUWM010000099        10014 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHUWM010000099 DAHUWM010000000
VERSION     DAHUWM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.100"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(29..181)
                     /locus_tag="NK788_001738"
     CDS             complement(29..181)
                     /locus_tag="NK788_001738"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCH6770066.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(372..1250)
                     /locus_tag="NK788_001739"
     CDS             complement(372..1250)
                     /locus_tag="NK788_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6770067.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1289..2407)
                     /locus_tag="NK788_001740"
     CDS             complement(1289..2407)
                     /locus_tag="NK788_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCH6770068.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2404..4503)
                     /locus_tag="NK788_001741"
     CDS             complement(2404..4503)
                     /locus_tag="NK788_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCH6770069.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4554..5375)
                     /locus_tag="NK788_001742"
     CDS             complement(4554..5375)
                     /locus_tag="NK788_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6770070.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5372..6181)
                     /locus_tag="NK788_001743"
     CDS             complement(5372..6181)
                     /locus_tag="NK788_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770071.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6202..6696)
                     /locus_tag="NK788_001744"
     CDS             complement(6202..6696)
                     /locus_tag="NK788_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770072.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6708..7196)
                     /locus_tag="NK788_001745"
     CDS             complement(6708..7196)
                     /locus_tag="NK788_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6770073.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7273..9969)
                     /locus_tag="NK788_001746"
     CDS             complement(7273..9969)
                     /locus_tag="NK788_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6770074.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3041 a         2259 c         1653 g         3061 t
ORIGIN      
        1 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
       61 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      121 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      181 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      241 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      301 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      361 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      421 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      481 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      541 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      601 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      661 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      721 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      781 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      841 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      901 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
      961 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1021 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1081 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1141 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1201 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1261 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1321 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1381 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1441 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1501 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1561 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1621 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1681 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1741 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1801 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1861 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1921 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     1981 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2041 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2101 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2161 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2221 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2281 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2341 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2401 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2461 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2521 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2581 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2641 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2701 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2761 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2821 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2881 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     2941 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3001 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3061 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3121 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3181 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3241 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3301 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3361 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3421 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3481 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3541 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3601 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3661 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3721 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3781 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3841 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3901 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     3961 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4021 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4081 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4141 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4201 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4261 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4321 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4381 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4441 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4501 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4561 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4621 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4681 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4741 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4801 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4861 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4921 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     4981 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5041 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5101 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5161 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5221 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5281 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5341 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5401 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5461 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5521 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5581 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5641 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5701 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5761 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5821 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5881 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     5941 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6001 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6061 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6121 acatttcatg tccagcaatt ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6181 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6241 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6301 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6361 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6421 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6481 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6541 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6601 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6661 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6721 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6781 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6841 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6901 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     6961 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7021 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7081 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7141 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7201 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7261 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7321 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7381 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7441 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7501 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7561 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7621 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7681 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7741 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7801 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7861 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7921 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     7981 attgattgaa tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt
     8041 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8101 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8161 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8221 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8281 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8341 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8401 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8461 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8521 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8581 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8641 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8701 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8761 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8821 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8881 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     8941 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9001 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9061 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9121 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9181 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9241 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9301 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9361 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9421 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9481 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9541 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9601 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9661 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9721 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9781 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9841 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9901 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     9961 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatc
//
LOCUS       DAHUWM010000100         9940 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHUWM010000100 DAHUWM010000000
VERSION     DAHUWM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.101"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(111..566)
                     /locus_tag="NK788_001747"
     CDS             complement(111..566)
                     /locus_tag="NK788_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770075.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="NK788_001748"
     CDS             complement(720..1928)
                     /locus_tag="NK788_001748"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCH6770076.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="NK788_001749"
     CDS             complement(2040..2372)
                     /locus_tag="NK788_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCH6770077.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="NK788_001750"
     CDS             2520..3395
                     /locus_tag="NK788_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6770078.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="NK788_001751"
     CDS             complement(3477..4058)
                     /locus_tag="NK788_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCH6770079.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="NK788_001752"
     CDS             complement(4064..5323)
                     /locus_tag="NK788_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCH6770080.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="NK788_001753"
     CDS             complement(5325..6371)
                     /locus_tag="NK788_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770081.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="NK788_001754"
     CDS             complement(6606..6881)
                     /locus_tag="NK788_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCH6770082.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="NK788_001755"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="NK788_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCH6770083.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="NK788_001756"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="NK788_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCH6770084.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         2771 a         2113 c         1669 g         3387 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctggt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt
//
LOCUS       DAHUWM010000101         9923 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHUWM010000101 DAHUWM010000000
VERSION     DAHUWM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.102"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            5..907
                     /gene="gnd"
                     /locus_tag="NK788_001757"
     CDS             5..907
                     /gene="gnd"
                     /locus_tag="NK788_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCH6770085.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            909..1910
                     /locus_tag="NK788_001758"
     CDS             909..1910
                     /locus_tag="NK788_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCH6770086.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1907..2191
                     /locus_tag="NK788_001759"
     CDS             1907..2191
                     /locus_tag="NK788_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCH6770087.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2224..3021
                     /locus_tag="NK788_001760"
     CDS             2224..3021
                     /locus_tag="NK788_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6770088.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3035..3868
                     /locus_tag="NK788_001761"
     CDS             3035..3868
                     /locus_tag="NK788_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770089.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3886..4743
                     /locus_tag="NK788_001762"
     CDS             3886..4743
                     /locus_tag="NK788_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6770090.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4765..5274
                     /locus_tag="NK788_001763"
     CDS             4765..5274
                     /locus_tag="NK788_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770091.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5311..5712
                     /locus_tag="NK788_001764"
     CDS             5311..5712
                     /locus_tag="NK788_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770092.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5824..6177
                     /locus_tag="NK788_001765"
     CDS             5824..6177
                     /locus_tag="NK788_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770093.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6211..6654
                     /locus_tag="NK788_001766"
     CDS             6211..6654
                     /locus_tag="NK788_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCH6770094.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6671..7756
                     /gene="uxuA"
                     /locus_tag="NK788_001767"
     CDS             6671..7756
                     /gene="uxuA"
                     /locus_tag="NK788_001767"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCH6770095.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7749..8462
                     /locus_tag="NK788_001768"
     CDS             7749..8462
                     /locus_tag="NK788_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6770096.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8669..8818)
                     /locus_tag="NK788_001769"
     CDS             complement(8669..8818)
                     /locus_tag="NK788_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770097.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9214..9414
                     /locus_tag="NK788_001770"
     CDS             9214..9414
                     /locus_tag="NK788_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6770098.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3348 a         1544 c         2190 g         2841 t
ORIGIN      
        1 tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt
       61 aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc
      121 aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca
      181 gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa
      241 actttttcca aaactcggac caggagacat tattattgaa ggcggaaact cttattataa
      301 agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg
      361 gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa
      421 agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct
      481 gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata
      541 tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt
      601 tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat
      661 ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt
      721 ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt
      781 tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc
      841 agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg
      901 tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct
      961 ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc
     1021 aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa
     1081 gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt
     1141 cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa
     1201 tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta
     1261 atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat
     1321 gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg
     1381 gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg
     1441 ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag
     1501 tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag
     1561 aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc
     1621 tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa
     1681 gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc
     1741 atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg
     1801 aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc
     1861 ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat
     1921 ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat
     1981 ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg
     2041 aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca
     2101 atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac
     2161 aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat
     2221 ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct
     2281 agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa
     2341 gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg
     2401 attgattccc ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat
     2461 gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca
     2521 caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa
     2581 gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct
     2641 gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct
     2701 atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa
     2761 gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag
     2821 ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt
     2881 ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg
     2941 catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa
     3001 ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag
     3061 tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac
     3121 gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga
     3181 agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc
     3241 tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc
     3301 attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct
     3361 cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga
     3421 caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac
     3481 ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc
     3541 attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt
     3601 agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa
     3661 agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga
     3721 tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc
     3781 gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga
     3841 cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg
     3901 gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg
     3961 agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta
     4021 ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca
     4081 gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt
     4141 actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag
     4201 atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca
     4261 ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta
     4321 ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg
     4381 tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct
     4441 acaggcctaa agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg
     4501 ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt
     4561 gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata
     4621 ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc
     4681 acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta
     4741 taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt
     4801 ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt
     4861 gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga
     4921 agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat
     4981 gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac
     5041 gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa
     5101 aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa
     5161 aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca
     5221 tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat
     5281 gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg
     5341 gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt
     5401 ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac
     5461 gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa
     5521 gttatctgta cttattacga tacacccgtt catgcattag gaagttattt catgtttgat
     5581 tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc
     5641 agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg
     5701 ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga
     5761 aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta
     5821 acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat
     5881 gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct
     5941 attctttttt atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat
     6001 ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat
     6061 tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta
     6121 cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta
     6181 aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt
     6241 aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt
     6301 gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa
     6361 aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca
     6421 agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc
     6481 acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg
     6541 cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta
     6601 actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa
     6661 ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt
     6721 aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat
     6781 gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca
     6841 aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac
     6901 agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg
     6961 tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt
     7021 gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca
     7081 gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc
     7141 gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat
     7201 gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg
     7261 cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg
     7321 ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc
     7381 tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt
     7441 agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga
     7501 gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga
     7561 tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga
     7621 tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc
     7681 aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg
     7741 agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa
     7801 ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc
     7861 gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg
     7921 gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata
     7981 aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt
     8041 tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa
     8101 aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag
     8161 gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa
     8221 tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag
     8281 caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc
     8341 ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact
     8401 atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct
     8461 aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag
     8521 atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag
     8581 aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga
     8641 gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt
     8701 gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata
     8761 gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag
     8821 ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc
     8881 atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag
     8941 ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact
     9001 ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt
     9061 gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag
     9121 cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa
     9181 cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc
     9241 aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat
     9301 ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac
     9361 gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa
     9421 ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg
     9481 gtcgttgaat gggctgaata gtagtgtata aaacaatttt gtggagcgtg aagcttttat
     9541 ggtagaggaa aatacgaata aagtgatgtt gataaaaaga ttcctatctt gaatatgaat
     9601 cgagatgtct tgaaaatgaa tgagtttgac caatcattat cctatcgaaa tttacagttt
     9661 cgttcgattg gtctgtcaat taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc
     9721 ggcacatcta aatttttaag taacggacag ttcaaatatc aagcattgaa agaggctatc
     9781 ctactgtttg aaggaatacg aatatataaa aacgacaaag aggaaaaagt agttcattaa
     9841 atcccttcaa gtgatgaaag aatgagatct aataaaacct gatctttcat ctgttttttt
     9901 aataacgtaa taattttttt att
//
LOCUS       DAHUWM010000102         9889 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHUWM010000102 DAHUWM010000000
VERSION     DAHUWM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.103"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(50..562)
                     /locus_tag="NK788_001771"
     CDS             complement(50..562)
                     /locus_tag="NK788_001771"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCH6770099.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(576..2873)
                     /gene="recJ"
                     /locus_tag="NK788_001772"
     CDS             complement(576..2873)
                     /gene="recJ"
                     /locus_tag="NK788_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCH6770100.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3015..3458)
                     /locus_tag="NK788_001773"
     CDS             complement(3015..3458)
                     /locus_tag="NK788_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCH6770101.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3459..4247)
                     /locus_tag="NK788_001774"
     CDS             complement(3459..4247)
                     /locus_tag="NK788_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6770102.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4244..5185)
                     /locus_tag="NK788_001775"
     CDS             complement(4244..5185)
                     /locus_tag="NK788_001775"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCH6770103.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5362..5949)
                     /locus_tag="NK788_001776"
     CDS             complement(5362..5949)
                     /locus_tag="NK788_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCH6770104.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6170..7477)
                     /gene="obgE"
                     /locus_tag="NK788_001777"
     CDS             complement(6170..7477)
                     /gene="obgE"
                     /locus_tag="NK788_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCH6770105.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7771..8781
                     /gene="gap"
                     /locus_tag="NK788_001778"
     CDS             7771..8781
                     /gene="gap"
                     /locus_tag="NK788_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCH6770106.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8866..9801)
                     /locus_tag="NK788_001779"
     CDS             complement(8866..9801)
                     /locus_tag="NK788_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6770107.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2721 a         2266 c         1707 g         3195 t
ORIGIN      
        1 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
       61 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
      121 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
      181 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
      241 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
      301 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
      361 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
      421 gtttttcttt tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg
      481 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
      541 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
      601 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg
      661 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
      721 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
      781 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
      841 tgagaatgga tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
      901 taggcctcat caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
      961 acttctttca gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt
     1021 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     1081 ataatctcag gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc
     1141 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     1201 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     1261 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     1321 aaagcgaccg catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg
     1381 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     1441 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     1501 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     1561 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     1621 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     1681 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     1741 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     1801 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     1861 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     1921 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     1981 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     2041 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     2101 tgaagtccta tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga
     2161 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     2221 tctgcaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     2281 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     2341 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     2401 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     2461 ccttcttcga tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga
     2521 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     2581 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     2641 acagcttctt ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt
     2701 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     2761 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     2821 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     2881 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     2941 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     3001 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     3061 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     3121 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     3181 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     3241 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     3301 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     3361 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     3421 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     3481 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     3541 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     3601 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     3661 gccttatcga agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     3721 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     3781 gaataaaccg ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata
     3841 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     3901 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     3961 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     4021 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     4081 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     4141 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     4201 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     4261 cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt
     4321 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     4381 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     4441 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     4501 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     4561 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     4621 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     4681 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     4741 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     4801 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     4861 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     4921 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     4981 tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg
     5041 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     5101 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     5161 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     5221 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     5281 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     5341 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     5401 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     5461 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc
     5521 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     5581 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     5641 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     5701 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     5761 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     5821 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     5881 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
     5941 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa
     6001 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
     6061 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
     6121 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
     6181 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
     6241 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
     6301 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
     6361 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
     6421 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
     6481 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
     6541 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
     6601 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
     6661 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
     6721 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
     6781 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
     6841 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga
     6901 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
     6961 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
     7021 gttcgatttt tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc
     7081 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
     7141 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
     7201 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
     7261 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
     7321 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
     7381 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
     7441 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
     7501 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
     7561 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
     7621 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
     7681 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
     7741 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
     7801 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
     7861 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
     7921 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
     7981 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
     8041 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
     8101 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
     8161 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
     8221 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
     8281 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
     8341 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
     8401 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
     8461 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
     8521 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
     8581 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
     8641 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
     8701 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
     8761 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
     8821 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
     8881 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
     8941 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
     9001 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
     9061 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
     9121 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
     9181 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
     9241 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
     9301 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
     9361 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
     9421 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
     9481 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
     9541 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
     9601 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
     9661 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
     9721 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
     9781 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
     9841 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAHUWM010000103         9632 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHUWM010000103 DAHUWM010000000
VERSION     DAHUWM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.104"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(16..678)
                     /locus_tag="NK788_001780"
     CDS             complement(16..678)
                     /locus_tag="NK788_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770108.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(691..2193)
                     /locus_tag="NK788_001781"
     CDS             complement(691..2193)
                     /locus_tag="NK788_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770109.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2741..4420
                     /locus_tag="NK788_001782"
     CDS             2741..4420
                     /locus_tag="NK788_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCH6770110.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4547..5026
                     /locus_tag="NK788_001783"
     CDS             4547..5026
                     /locus_tag="NK788_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCH6770111.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5245..5925
                     /locus_tag="NK788_001784"
     CDS             5245..5925
                     /locus_tag="NK788_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCH6770112.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5933..7264
                     /locus_tag="NK788_001785"
     CDS             5933..7264
                     /locus_tag="NK788_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCH6770113.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7279..8241
                     /locus_tag="NK788_001786"
     CDS             7279..8241
                     /locus_tag="NK788_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCH6770114.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8352..8690
                     /locus_tag="NK788_001787"
     CDS             8352..8690
                     /locus_tag="NK788_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCH6770115.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8769..9476
                     /locus_tag="NK788_001788"
     CDS             8769..9476
                     /locus_tag="NK788_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCH6770116.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3058 a         1736 c         2071 g         2767 t
ORIGIN      
        1 tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac
       61 ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg
      121 ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt
      181 tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc
      241 gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc
      301 aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt
      361 agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta
      421 agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag
      481 tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga
      541 aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc
      601 gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa
      661 ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa
      721 tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc
      781 ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga
      841 aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg
      901 tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt
      961 ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc
     1021 aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt
     1081 acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt
     1141 aatcataatg atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt
     1201 tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt
     1261 tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc
     1321 cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt
     1381 cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa
     1441 gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt
     1501 catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc
     1561 caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct
     1621 gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg
     1681 tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc
     1741 cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc
     1801 agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga
     1861 ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt
     1921 aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg
     1981 ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc
     2041 tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac
     2101 aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt
     2161 tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat
     2221 ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca
     2281 acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg
     2341 aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct
     2401 agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat
     2461 ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag tattctggct
     2521 atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc
     2581 caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa
     2641 tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg
     2701 acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat
     2761 cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga
     2821 tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt
     2881 cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt
     2941 aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt
     3001 accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga
     3061 agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg
     3121 tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa
     3181 tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc
     3241 tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt
     3301 agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca
     3361 aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg
     3421 tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg
     3481 aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga
     3541 aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga
     3601 tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca
     3661 aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat
     3721 cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta
     3781 tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa
     3841 tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca
     3901 aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata
     3961 caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag
     4021 tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat
     4081 cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt
     4141 tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg
     4201 attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac
     4261 aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat
     4321 ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc
     4381 agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac
     4441 ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca
     4501 gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac
     4561 aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca
     4621 tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat
     4681 atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt
     4741 gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt
     4801 tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat
     4861 tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa
     4921 agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga
     4981 tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga
     5041 agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc
     5101 tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt
     5161 tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata
     5221 cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca
     5281 attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg
     5341 acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt
     5401 gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt
     5461 gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt
     5521 ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc
     5581 tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca
     5641 gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt
     5701 gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg
     5761 ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat
     5821 tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat
     5881 agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac
     5941 aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt
     6001 aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga
     6061 tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg
     6121 caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg
     6181 ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc
     6241 gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa
     6301 ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg
     6361 attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg
     6421 cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt
     6481 gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta
     6541 tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc
     6601 gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt
     6661 tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt
     6721 aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt
     6781 catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat
     6841 gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat
     6901 atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa
     6961 gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt
     7021 acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa
     7081 tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt
     7141 agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt
     7201 gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc
     7261 ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac
     7321 gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta
     7381 ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa
     7441 acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa
     7501 ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat
     7561 cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat
     7621 cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg
     7681 atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc
     7741 gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct
     7801 tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg
     7861 ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat
     7921 ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca
     7981 gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt
     8041 tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc
     8101 aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca
     8161 aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat
     8221 aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac
     8281 aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa
     8341 ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag
     8401 atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa
     8461 ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg
     8521 atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag
     8581 gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag
     8641 aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca
     8701 aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg
     8761 aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca
     8821 ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat
     8881 acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac
     8941 aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact
     9001 acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg
     9061 ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg
     9121 tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt
     9181 tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga
     9241 ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg
     9301 cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac
     9361 cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga
     9421 cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata
     9481 aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata
     9541 ccatttattt ggaataaggg ctctttgtca ataaggactg atgggttgta caaaatcaaa
     9601 tctgggtcag aatgaacctc attctggctc ag
//
LOCUS       DAHUWM010000104         9598 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHUWM010000104 DAHUWM010000000
VERSION     DAHUWM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.105"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(169..906)
                     /locus_tag="NK788_001789"
     CDS             complement(169..906)
                     /locus_tag="NK788_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6770117.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(973..1542)
                     /locus_tag="NK788_001790"
     CDS             complement(973..1542)
                     /locus_tag="NK788_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCH6770118.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1670..2542
                     /locus_tag="NK788_001791"
     CDS             1670..2542
                     /locus_tag="NK788_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6770119.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            2884..3756
                     /locus_tag="NK788_001792"
     CDS             2884..3756
                     /locus_tag="NK788_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6770120.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            3753..5711
                     /locus_tag="NK788_001793"
     CDS             3753..5711
                     /locus_tag="NK788_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6770121.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(6043..6894)
                     /locus_tag="NK788_001794"
     CDS             complement(6043..6894)
                     /locus_tag="NK788_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6770122.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            7017..7484
                     /locus_tag="NK788_001795"
     CDS             7017..7484
                     /locus_tag="NK788_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCH6770123.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(7747..8229)
                     /locus_tag="NK788_001796"
     CDS             complement(7747..8229)
                     /locus_tag="NK788_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCH6770124.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(8329..9192)
                     /locus_tag="NK788_001797"
     CDS             complement(8329..9192)
                     /locus_tag="NK788_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCH6770125.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2829 a         1978 c         1745 g         3046 t
ORIGIN      
        1 caagtcaatg tccatattta aataacattt gtttcttttt aataaatttg ctttttctgc
       61 ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg atggtcgttt
      121 gaataactat ttaacaagta attctctctg gatattatcc gttatggatt atacttcctg
      181 tgtcgtcgga cggataatca tgttgctgac agacatccgg tctggcgttt ctatcgcaaa
      241 tgctacagca gaagctatat cttcagatgt taatccccat tctttttgcg cttcatgaag
      301 ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa cagctcctgg
      361 agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc cttccatgat
      421 tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa caacatgtcc
      481 cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga ttggaagtac
      541 ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt gccattcatc
      601 acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt acaatacatc
      661 gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg caaaatctct
      721 tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta ttgcttttag
      781 gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta atagcttcgt
      841 tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt ctgataaatc
      901 attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact atctcttatt
      961 tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag ggattcctgc
     1021 aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa caatggcatt
     1081 ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag catttttctt
     1141 gatgtggatt ggctttactg acaagcctct tcgttctttt ggatcggtta gatggttcac
     1201 cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga tgccacctaa
     1261 gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa tatgtttgcc
     1321 gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt cactaatgat
     1381 attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca gcgctgctaa
     1441 aggctcttcg ttttccgctt ggatctgatg gatcacttca aagagcggat ccgttactcg
     1501 aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc ctccttttat
     1561 ccttacttta taacggacgg cttatgatgt ctaatactta taaaacatac ttaactattc
     1621 cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa tggatattcg
     1681 tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag cagcaaaatc
     1741 tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag aagaacttgg
     1801 tgtgacactt ttcactcgag gctcccgaga gatacagttg acggattctg gtcgatatct
     1861 ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa
     1921 tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag caatctcttt
     1981 catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt ttcatttata
     2041 tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg attttggact
     2101 cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag cccaagatcg
     2161 ttggggactt ttgatacatc ggaatcatcc tttagcaaag caaacagtca tccatccgca
     2221 ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca gtcaactagc
     2281 tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata acttattgta
     2341 caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag atgggatcat
     2401 caatacttat gggacggaaa aagtattcat cccactcgat ccgccattac aagcttcact
     2461 gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat ttttagatgt
     2521 cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct ttttaacaac
     2581 gaataagtta agcaaaagat ccaatgatta aataacataa gtttcttaat ttgttccttc
     2641 cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat tgacaaaaca
     2701 taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga ctagatcata
     2761 ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg tgtctatttt
     2821 tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg
     2881 cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga cggtggacag
     2941 gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga tttgattttt
     3001 gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat
     3061 ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat tgatcatgcg
     3121 gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac cgaccacatt
     3181 gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact tttaagagaa
     3241 atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga gtatctgaca
     3301 aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat ccatattcac
     3361 agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac gattgtatct
     3421 gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga accattttca
     3481 ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac ccatgcgcaa
     3541 caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata cccagaaagc
     3601 tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg
     3661 aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc aatcgtacaa
     3721 acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa cgaatgggac
     3781 gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta gggatcggaa
     3841 actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct ggcggtaacg
     3901 cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg
     3961 acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt
     4021 taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt gatccatctt
     4081 ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg gcagctcttt
     4141 ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc ggcaagcgtc
     4201 tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat
     4261 ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt ttaggctggg
     4321 gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga ttgcatcaag
     4381 caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt aaatttttgg
     4441 cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc ccaatcatga
     4501 tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag
     4561 caaaaacagc ttccttgatg ttagcagcag cttttgcttc attctttact ggtgtaacag
     4621 aaccacttga attctcattt atgttcgttg cttggccatt atatgtcttg catgcaattt
     4681 tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga tttgccttta
     4741 gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat cagccattga
     4801 tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt aactttgcta
     4861 tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca gatgacgata
     4921 ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc tacgctgcat
     4981 taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt cgtttgcaag
     5041 tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta cctggtatga
     5101 agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag
     5161 atgagatggc aaaactacat ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg
     5221 ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga tatttacgct atcgcaaatg
     5281 ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg
     5341 acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct ggtacgatca
     5401 caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt atcgaagtct
     5461 tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact ttatatgtaa
     5521 aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt gccgctattc
     5581 aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac aaagtccaaa
     5641 gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca
     5701 acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac aggagctgag
     5761 acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct gccaactccg
     5821 cggtaataag tccagccaaa cgtaacatat gaagagctat tttttatttg actcttctta
     5881 ttacttgatg cagaacagct tttttacaac ctctgataaa cggtgttcaa aaaccagttt
     5941 atcggtatga agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     6001 catactcaaa actaatcggc tttttcacaa cctctttata cattattcct gatccaatat
     6061 ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt ctaaatcatc
     6121 agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa acatgtcagc
     6181 tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat cacttgcttc
     6241 aataatttct aactttttac ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc
     6301 agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta gtacttttgc
     6361 tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc ctgcagcata
     6421 aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct ctacatacca
     6481 attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt cagtgaattg
     6541 gtgatctggc gctaatacac tgatcgagtg atcggcatct gcaaaacttg tataagcgat
     6601 ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg cttgacgatt
     6661 tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt ctttcattga
     6721 gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca gtgcttctcc
     6781 tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct tttttaagaa
     6841 ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat gcatatttgc
     6901 cactccctat tctttttatt tgttaagatt tacagctgta catttataaa ttacaccttt
     6961 atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga caactgatga
     7021 agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa atgtttcaag
     7081 atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat gcagcaaatg
     7141 tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac
     7201 cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc tatcgcagca
     7261 tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg
     7321 gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag
     7381 aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt ttggaaaaag
     7441 aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct ctactgtgcc
     7501 tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg gacaaaagcc
     7561 ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc atgacgtctt
     7621 gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga
     7681 aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt cacgacctct
     7741 atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt tcccgttggt
     7801 aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt ctacttctct
     7861 taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag gcgattctgc
     7921 taaagcgatt acaacaagat acgttccttt tgggaatggg atgattcggg tagtgtttgg
     7981 aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat agggttcgtt
     8041 gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg ttccattacc
     8101 gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct
     8161 actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt cgatcctata
     8221 ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa aattaaaggg
     8281 aaatgacacg attacgctct tttttctagg gatattttta ttttttaact atttttttgc
     8341 tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc attttgtaaa
     8401 agagatctcg ggagcttcga ctaactcata atctgaagaa ggaagccatt ctgcatagat
     8461 tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa aaatggccca
     8521 agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt ttgtcgtagc
     8581 aaagccaatc atgtgcatca aactgccctt ttcttccaag cgggctccat caaatccata
     8641 agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct cttccctttg
     8701 agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt caaatttgac
     8761 aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga tacgaaattc
     8821 catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag atttttgtat
     8881 gccgtatctg ctgatttctg aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa
     8941 accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg atccatctag
     9001 gagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta gcgacatccc
     9061 tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat tttcggctat
     9121 tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca attggttcag
     9181 atctttaagc atatttatct tcctccttgc ttgccttttt atcatattat caaagaaagt
     9241 caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg tatggatcta
     9301 aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc tatctatgtt
     9361 ttacctttag aaaaataaat tttggggaag acacattcta agtaaacaaa gcggtaaaaa
     9421 tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa attgttgttc
     9481 aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac ctcttttatc
     9541 aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat aaataaaa
//
LOCUS       DAHUWM010000105         9567 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHUWM010000105 DAHUWM010000000
VERSION     DAHUWM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.106"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..451
                     /locus_tag="NK788_001798"
     CDS             <1..451
                     /locus_tag="NK788_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6770126.1"
                     /translation="IEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHLIDVIGNYPET
                     SITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKYGRKVYESHNY
                     LHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            544..1706
                     /locus_tag="NK788_001799"
     CDS             join(544..820,820..1706)
                     /locus_tag="NK788_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6770127.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            1986..2966
                     /locus_tag="NK788_001800"
     CDS             1986..2966
                     /locus_tag="NK788_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6770128.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3088..4506
                     /locus_tag="NK788_001801"
     CDS             3088..4506
                     /locus_tag="NK788_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCH6770129.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4577..6046
                     /locus_tag="NK788_001802"
     CDS             4577..6046
                     /locus_tag="NK788_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6770130.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6056..6523
                     /locus_tag="NK788_001803"
     CDS             6056..6523
                     /locus_tag="NK788_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6770131.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6559..7431
                     /locus_tag="NK788_001804"
     CDS             6559..7431
                     /locus_tag="NK788_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6770132.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7512..>9567
                     /locus_tag="NK788_001805"
     CDS             7512..>9567
                     /locus_tag="NK788_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6770133.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLS"
BASE COUNT         3322 a         1399 c         1924 g         2922 t
ORIGIN      
        1 tatagaagaa ttcatcaaag taaataattt aatgacccat attcaaaaac gaccaatcag
       61 aataactaat gatctaaaac tcagtacgag tatgattcat ttgattgatg tgattggaaa
      121 ttatcctgaa actagtatta cggaattagc tgatcgatta ggcgttacca aaggagctgt
      181 atcccaacag attcccactt tagagaaaat gaatcttata agcatttctc aaaaaaaagg
      241 aaataagaaa actaaacttg tatcgttaac aaaatatggt cgaaaggtgt acgaatcaca
      301 taattattta catgaagagc tttatagctc aataaatgaa gcgttagaat cattctcacc
      361 agaccaaatt tcaaccatta aacagattct agaaaaagta tcttatacaa tcaatgaata
      421 tcaagtaagt ctgacaagaa aggaagattg acatgaaaaa aatgattttt gaatcggcta
      481 cactagacta gacagaaaaa ataaggtgtg taaaataaat gaaaacacac tggaggaatt
      541 tttatgtcaa gacgtcaacg tagaacctat tcaaaagaat tcaaacaaca agtcgtcgat
      601 ctttatctcg caggaaagcc ccgagctgaa atcattcgag attatgagct gactacctcg
      661 gcctttgata agtgggtcaa gcaagcgcaa actactggct cgtttcagga aaaagataac
      721 ttgatccctg aacaagtaga actaattgca ttacgaaaga aaaataaaca gcttgaaatg
      781 gagaatgaca ttttaaagca agcggcgctg atattgggac gaaaagacaa gtaattgata
      841 ccaataagca caaatactca atatcagcga tgtgtaaagt gttaaacatc tcttgccaaa
      901 cctattatta tcaagccaaa ccagttgaag atgaatcaga actggaagaa ctcgttcaag
      961 aagaatttat tcgcaatcga aaagcttatg gtaccaggaa gctgaagcag tgcttggcca
     1021 atctaggtct tcaagtgagt caacgcagaa tcggccgtat gatgaaacag cgtggcttgc
     1081 gttcaacata cacattggct catttcaaat tccattcttc tacttgtaac gaagccaaga
     1141 ccgccaatat tctagacgga accttcctac aggaaaagtc attagaagcg attgtaaccg
     1201 accttaccta tgttcgtgtt gggaagaaat ggcagtatat ctgcctgatt cttgatttgt
     1261 ttaatcgaga aatcatcggc tattcatgcg gggagaagaa agatgcgacg ttagtcaagg
     1321 aagcttttgc aaagattccg tactcgttga ataatgtaca actttttcac acggatcgtg
     1381 gaaaagaatt cgataaccag acgattgacg aaattttatg ggcctttaat atcacgcgtt
     1441 ccttaagcaa aaaaggctgc ccctatgata acgccgttgc agaatccacc tataaatctg
     1501 tcaaagtaga attcgttcat caataccgct ttgagacatt ggctaatttg cgattagagc
     1561 tatttgatta cgtccattgg tggaatacca tccggctgca cggcacgtta ggctatgaga
     1621 caccgatcag ttttcgacag cagagattgg cgcagcgaat ccttgataat gagctcggat
     1681 gtgataccgg tggcgaggca ccttaattat ttgaatgagc ttctgcctta gagaatcaga
     1741 ttcgaggagg ctcgttgtca agggcagtcg gaacaccagc agcaagctta cgcaatacct
     1801 tataattttt gtcaaaaaaa gtgttgccat tccacatata tctttaaaca gctattgcca
     1861 gataaaaata aatgatatta tatatataaa tacttatccg aaaaggggaa tgagagagtc
     1921 aaggttcttg gaatgtcgaa tagtcgagta ggtagatttt ctatctacaa ccttgttgtt
     1981 taaaaatgaa aaaaattgga attagagaag ttgcgaaaga ggcaaatgtt tctcctacga
     2041 ctgtgtcgag ggtattgaat gatcgtggat atttgagtga ggaaacaaaa agaaaagttt
     2101 ttgatgccat gaaaaaatta gaatactatc ctaatgaact ggcgagagca ctttttaaaa
     2161 acgaaacata taccgtaggt ttgatttttc ctacaattac taatccattt cacgctgagt
     2221 taattcaaga tatcgagttc gagctttcca gcaggggata taaagtgttg ctgtgcaata
     2281 gcttaaacaa tccaacaaaa gaaaaagagt acttgacgat gttgagaaag aatcaggttg
     2341 atggaattat agtaggaacg cacaataaag atattgcgga ttatgatatt ccagggctac
     2401 ctattgtagc gattgataga agtcttggtg aaaatacagt tacagtatct tgtgataatt
     2461 acaggggtgg ggaattagct actcaactac ttgtaaatca tggctgtcag aatatactgt
     2521 gtattcgggg agatagtaag ataaagctac ccgcgaatag taggacttta gcatataaag
     2581 atgttttgaa taaattcatg ctggaaccgt tagtcctaga agtaccattt gtaaaagaaa
     2641 tcaatgaaaa aaatgaaata attaagacat atctggaaac tcatccaaat attgacgggg
     2701 tttttgcagg agatgattta ttggcttctt tggcgattaa caacttagga agttcgggca
     2761 aaagagttcc aaaagaggtt aaagtgattg gatttgatgg agctcaacaa acattgattt
     2821 ataatccaga attatcaact attcaacagc ctatagatca aatatctaaa gtagcagtaa
     2881 aaaaattatt gaatatgatt aagggcgaaa aggagacgga tgaactttca cttccagtaa
     2941 agctcataaa aaaaaataca gcataagtta aatcggcgaa aaattccttc gtcgatttaa
     3001 aaatattttt atgaaacggg ttgacatatg aaacgggttg acatatatta gttgtgaaga
     3061 taaaaacctt ttcactggag gaagaaaatg gagaaattat taattaaaag caataatcat
     3121 caactttact accaacctaa ggatgtttgg gtcggagatg ttatgcctta cgggaaagaa
     3181 ggaaaattct ttatttatca tcaacgtgat actagaaatc cagttccttt tggagaaccg
     3241 tttggctggg ctttagcaac tacgaaagat tttgttcatt ttgaagactt tggggaatct
     3301 cttttaagag gggatgacaa tgagagagac cagtttatct acgctggttc tgtatttgag
     3361 gcaaatggaa aggttcacgc attttataca gggtataatc gtgaattctt agcgcaggga
     3421 aagacatctc aagtgttgct tcatgctgag agtgatgatt ttgttcattg ggaaaaatca
     3481 gaaacagcat tggagttaga accacaagaa gggtatgata ttcgaaactg gagagatcca
     3541 tttgtcgttt gggatgatga aaaagaagaa tatttgctaa ttttaggggc gcgaaagggt
     3601 caggataaac atctacaaac cggacgttta gtggaattta catcaaaaga tttaactaat
     3661 tgggaattca aaggtgattt ttgggctccg ggattgtata ccatgtttga aatgccagaa
     3721 cttttcaaga ttggtgactg gtggtatctt gtctattcag aatactcaga tggaaataaa
     3781 atactctatc gaatgagtca aagtttggaa ggtccttgga ttaagcctaa agatgaagct
     3841 tttgatggac gggcttatta tgcaggtaga acagcttatg atggaaaacg tcgagtccta
     3901 tttggatggg taccaacaaa agatcaagaa aatgataaaa acaattattt atggggaggt
     3961 actttggttc ctcatgaagt ctatcaaaaa gaggattttt cacttggggt aaaacccata
     4021 gacgaagtgt ggaatgcatt cataaattta catgatattg ccgatgtaga gatagaaaca
     4081 gcagacacaa gagaagaagt aacgcttgtc aaaaatagtg gaactttgtt taatttagaa
     4141 acaacaattt catttgatga gaatcttagt gacttttcac tgagattata taaaaacctt
     4201 gagacagatg aatcttacga atatcgcttc aatttagatg aggggcgtct tacatttgat
     4261 aaaaaccctt gttacccttg gtttcaatat atggataaag gacttattag acctatatct
     4321 ttaaagccta accaatctta taaactaaaa gtgattgttg atgacaatat aatgacactt
     4381 tatattgatg gaatcgctct taacgctcgt gtacatggtt gttttggtca agcaattact
     4441 attacagcaa caaatggatc aatgactgca ataaatacca agttttcaaa tgaaataaag
     4501 aaataataaa cgtcaatcat gtggcaggtc tgaaaatcaa acatgacctg tcacatttaa
     4561 aagggaggat aaatgaatgg actacaaaaa aattgccgaa gatgtattgg aaaacgttgg
     4621 aggaaaagag aatatctcaa gcgctacaca ctgcattact cgtttacgtt taattcttaa
     4681 agacaaaagc aaagcgaata tggaaatgct tgaggaaata gaaggtgcaa aaggtgttct
     4741 tttcaatagt ggacagttac aagtaatttt cggaacaggt ataattgaaa aggtttacaa
     4801 agagttcgtt gaattgacag gagctaagga agtatccgtt tctgaaatga aagacgaagg
     4861 agtcaataat tcaggaaata gacttcaacg agcttttaaa gttttctcgg atatctttat
     4921 tccgatcatt ccagctttta ttggggcagc aatggtttta ggaattcgtt cgttgattac
     4981 cactcctgga atgtttggaa tggatggctc attcgctgat cacaatgtat tcctaaatga
     5041 tttatccaaa tttctgggta ttattgcaac cacatttgat tatttaccag ttttagttat
     5101 gtattctgca acaaaacgtt ttggcggaaa ccctatttta ggattacttg taggatttgt
     5161 tatgattcat ccaggactag ccaatcggaa tgatttcgtc atgggaacta taaagcctga
     5221 ttattggcat ttatttggat taagcattcc agcagttgcg tttcaagggg gtgtttttcc
     5281 agcaattttg acttcttggt ttttagcaaa agttgagaaa attacaaata aatattctcc
     5341 tcaagtactt gcttacattt tagtcccaac tgtgacaatt gttcttgcca actttgcact
     5401 atttttagtg tttggtccat tggggaattt tatcggtaat gttttaggtg gaatcattga
     5461 tttcctatat atgaagatgg gtgcctttgg tgcttttgtt tttgcaggcg cgttacaacc
     5521 attagttgta acaggaacac aacatgctat tcaaggtatt gaggcaaact tagtcgcaac
     5581 aactggattc aattatatcc aacctatttg gtcagtttca attattgccc aaggtggcgg
     5641 ttgtattggt atgtatttac tctttaagaa aaaatctaaa gatagagata ttgcgatttc
     5701 aagctttatt ccaacactgg taggtattac tgaaccggct atctttgctg ttaatttgaa
     5761 atattcaatc gttccttttg tttgctcgtt tattggtgct ggttttggtg gagcatttat
     5821 gaaaattttt gatgtaaaag ccattggaca aggattgact gtattgccag gtctgactat
     5881 tgctaatcca gttcttcctt atatatttgg taatctcata gcattcattt taccaatcat
     5941 ttttattctc gcttatgata aatataaagt gtttatccct gaagctaaac accgtaaatt
     6001 tgcagaaaaa actgactcaa aaaaattgcc agttcaagta aagtgagatg agagcatgtt
     6061 aaattttttt agaaaaaatg aaataagcgt tgtagcacct gcaaccggtg tttttgaaaa
     6121 actggagaaa ttaagtgatc cagtcttttc aaaaggaatg atgggacaag gatttgcaat
     6181 tgatgcaaaa aacgaaacga ttgtatctcc aatcgatggt tttgtttcaa gtatttttcc
     6241 tacaaaacat gccttaggaa taaaaacaaa atcaggaatt gagatacttg ttcatgtagg
     6301 aatagataca gttgagctaa atggtgaggg atttgatatt aaagttcaag aaggacaaaa
     6361 agttaaggct ggggatattc ttatgaccat taattttgta gtttttgaaa agaatcacaa
     6421 gactaaagat gtcattgttg tttttccaga ctaccaagac gaaattttca ttccgaaact
     6481 tgaagaagta aaacaaggac aagaattgtt tgttcttggc taatgataag taattaataa
     6541 tagaatcgag gaaaatgtat ggtttttggg gctattgaag ctggtggaac aaaatttgtt
     6601 tgtgccattg gagataaaga tttgaacata tttgaaaaag tcagctttcc cacgactaca
     6661 cccaaagaaa caatgaattt agttttagat ttttttaatc atcataaaga tgatttagaa
     6721 ggtatcggag tgggatcgtt cggtcctatt gatattcatt gtgaatctaa aacgtatggc
     6781 ttcattacat caacgccaaa acttgcttgg caggattttg attttattgg aagtttaaaa
     6841 aagcaactta atattccaac tgcttggacg actgatgtaa atgcctcttg ctatggtgaa
     6901 tatgtttctg gaaatggcag aggcctttca agtgttgttt actataccgt aggaacgggc
     6961 ataggaggtg gcgctatcca aaacggaaac ttcattgaag gttttagtca tccagaaatg
     7021 ggacatatgc ttgtaaaacg acacaataaa gacaactttt ctgggacatg tccattccac
     7081 ggagactgtt tagagggctt agctgctggt ccggcaatcg aaagaagatc agggaaaaag
     7141 ggacaagaag tgactgaaaa tgacgttact tgggaaatag aagcttatta cattgctcag
     7201 tgtgcatata atacaacatt gatgttatca cctgatgtca ttatatttgg tggcggagta
     7261 atgaaacaaa aacacttaaa aaagaaagta caagataagt ttattgagtt gatcaacgga
     7321 tatgtcgcta ttcccgatgt tgacaattat attatgacac caaaattgtt agataattct
     7381 ggtattgtag gttgtttagc tttagcaaga gacgttaaag aaaaatctta atttggaaaa
     7441 aagatcgtct cgactatttg tttggttgat gcgatctttt tttcaattag taattgaagg
     7501 gacgcctaaa agtgaaaaaa aaaatgaaag atgaagtatt agagtatatc aaaaataatt
     7561 taaagtatta cgatttttct gctcaagata ttgcaatgaa attttgtatt aaaagaaatg
     7621 tcgcttccca ctatttgaat cagttatttt cagatggaaa attactaaag aatgattctg
     7681 ttagaccagt aatgttcaag tataatcaac aaaagccaaa agactgcttt tctaagttta
     7741 ttggagcaga catatctttg aaatccacta tagataaatg caaagctact gtaatgtatc
     7801 cccctaatgg cctcccatta ataataaaag gaaacagtgg cgttggaaaa agttttttag
     7861 caagtttgat ctatcagtat gctcttgata gaaaagtaat tcataatgac gcaaaatttg
     7921 ttgttgtgaa ttgtgcagat tatgcaaata atccagagct attgtcagca gttttatttg
     7981 gatataaaaa aggagccttt actggggctg aaaaagatac tgcagggatg ctatcttctg
     8041 ctaatggagg ataccttttt ttagatgagg ttcacaatct aagtgcagaa aaccaagaga
     8101 aattgtttct tcttatggat tctcaaaagt atcgtaaact tggtgaaagt gtgcaatggg
     8161 agtatgcaaa tgttcgcctt atcttagcca caacagaaga taaaaatacc tctttgttag
     8221 caacttttag aagacgcatc ccagctgaaa tcactctgcc agattacaat aatagaagta
     8281 catctgaaaa aattcaatta ttatttgaat ttcttaaaga tgaagcaatt acgattgatt
     8341 caaaaatatt ttgttcgatt gaacttttta ctgatctttt atcgaaaaca tttgaaggaa
     8401 atgttggaga attgaggaat gaaatcaagc ttctttgtgc agaaggttat ttaggaaata
     8461 agagaaagtc ttcaatttat ttaggggaca aactagacag cggtttttgg atagatccag
     8521 aaattagtat agatttaaaa aaaatgtttc ttaaatcact ttctgaaatt gatttgattg
     8581 aactttttca aaatcacatt tcaattgaag aaagcatgag tcaattaaga gatagaatac
     8641 ttaaagagtg tgcaggttct tctgagtata attatttaga aagtgacgaa tttatagcat
     8701 taagaaacta tgttgttaat aaaatatcac caattattga ttcgacaggt ctatgtttat
     8761 tagatgaatt cttggcggat atcagtctat ttataatgtt tattgatgta attgaagaaa
     8821 catcaagaaa ttttttgctg tctaagttca gaaaatttcg tatgaaaaat gataaacaaa
     8881 aattattggc aaatgagatt tggagtagtt tagaagtcga agaaagtaaa caagaacttt
     8941 tgttgaaatg gatacttttt cttgtaaaga agttctatgt gaaaatacca gaaacccatt
     9001 gtctgatagt tatgcacggt aaaataacag cttctgcgat cgcaagggaa acaaataagt
     9061 tgctgaatac atatgtttat gaggctttcg atatgccaat tgaaggtgag actagtgact
     9121 taattaacct aatcaacaat ttttgtaaaa gtatagatac gacgaatgga ctcattctgc
     9181 tagttgatat gggatcattg gaacaaatgt atgaaaaaat tgaatccaat gtaataggtg
     9241 atttggtaat tttaaacaat gtttctacag ctttagcgat tgaatgtggg attcaagttt
     9301 gccaaaaaaa gccaattagt catttttacc agatggattt tgattcgttt caagttaaag
     9361 ttcagtatta taaaggatta tcacaaaaga aaaatgtaat agtatcttgt ctctctggtg
     9421 aggggatttc tgaaaaagtt aaggatattt taaaacggta tctcgataat caagtggaga
     9481 ttttgatttt tgattttaac gcattaaaga aaatagcaaa ggaaaaagat actatgattt
     9541 ttaagaatac aatttgtgtt ctttcaa
//
LOCUS       DAHUWM010000106         9302 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHUWM010000106 DAHUWM010000000
VERSION     DAHUWM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.107"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..338)
                     /locus_tag="NK788_001806"
     CDS             complement(<1..338)
                     /locus_tag="NK788_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCH6770134.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLV"
     gene            complement(328..2502)
                     /locus_tag="NK788_001807"
     CDS             complement(328..2502)
                     /locus_tag="NK788_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCH6770135.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2518..4860)
                     /locus_tag="NK788_001808"
     CDS             complement(2518..4860)
                     /locus_tag="NK788_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770136.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4882..7425)
                     /locus_tag="NK788_001809"
     CDS             complement(4882..7425)
                     /locus_tag="NK788_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6770137.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7435..7833)
                     /locus_tag="NK788_001810"
     CDS             complement(7435..7833)
                     /locus_tag="NK788_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6770138.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7914..8198)
                     /locus_tag="NK788_001811"
     CDS             complement(7914..8198)
                     /locus_tag="NK788_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770139.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8222..9220)
                     /locus_tag="NK788_001812"
     CDS             complement(8222..9220)
                     /locus_tag="NK788_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6770140.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2646 a         1995 c         1458 g         3203 t
ORIGIN      
        1 acgagaaaac ttgggacact tttaacatgg taacgttgta aaaaagtttg aagttcttct
       61 tgtcttggtt cttttcttgt ttcaattcga tatacattcc tagagaattt tttgtcgtat
      121 ctttccaaga aagattcaac ttcttggcat tttttacaat catttcttgc tagatacaca
      181 aaaaatgatt gattcgtttt ttgaagttct agtacttctt ctaaattact attgattagt
      241 tttccatttg attcttcgtt agtttttatc agtttaaaac aaccaaataa gaggaaacta
      301 acaataagta tcctaacaat aactttctta tttcgcatat tgtttctcca aatttggttt
      361 ccgaatatag acgactcgcg gtggttcctg gacgtcttca gctgttcgat acaccacaaa
      421 ttcgcctgtt ctcaatgctc gaatgcgatt cggattgaat ttaaagcggt ctacgtctcg
      481 tttcgtgcct ctttctcctg tccaatcaac ctttcccata tcagaataac ctgcttcttg
      541 tgtcgtcatt tccgttaatt caatgtcttc ttctgtcccc attaaatctg cccaataatc
      601 aatttctgtc gggtcatttg tcttcccaat aaagaacgta ttcacattcc caatcaactg
      661 ttttcgaagg acaggatcaa ttctatcaat gtccgcaagc gtttgagggg catacgccgc
      721 atgcacccca gcgcctcgtc ccttattgac taagtctata aggttatcat ttagataaga
      781 agctggttcg tcaaatatta ctaggacttt cgagcgattg gctgtttctc ctgcttgttt
      841 atacacttct gacgcaaaga aattaatatc tcccacaagc atttgagcca atgtccgaat
      901 aaactcacta taaatcaatc cgtttaacga gatataaaca atctttcctt ctttaacaac
      961 ttcttgaata tctaattctc tttttcctcc agaagtatca aagagttcac caagctcact
     1021 atccaataac atattgatat tggtctgtaa attctcagaa gtcgcataca aataaaacgg
     1081 gttcttctta tgttcgtaac gaacaaacaa ccgtttaaac aacgtttgct cgatttctgt
     1141 catgagagaa atgttttctt ttaataatag ataatacgtt tctaagtcaa cggttttacg
     1201 atcatgttca acgattcgct cttcacttaa ctgttccaca tcggcaaaat cacgttctgt
     1261 tttgtccatc gcttcattct ttttcgtttt tttcttcgtg gttttcttct tttctacgac
     1321 cactttttct ttaaataaat ctggattttt caactgaata cgatccgcaa acaaatgtaa
     1381 gacgtttcgt ggcaataaat aatgttgtaa ccaaactaga tttctttcaa ttttatggct
     1441 cttttggaac tcgtctaaaa gctgaatcgt tacctgtaat aacgctttgg ctgctgtcga
     1501 ataatacacg ctttctgttt ctgctagatt ggttaattta tctcgaatgg caatatcatt
     1561 gccatttttt acaggattat aacgcaaatc gccaccgtcc acaaagactt caatcgggcg
     1621 attgtatgcc tctgccactt cttttaacgc ttgtaaagtc tctcctgctc ctttcccatc
     1681 gactaagata acggggatac gaaacttaca cgcatgttct gctagtaatt ggatcaccgt
     1741 cgtttttcct gaacctgtag tcgctggaat aatcacatgt tggttcaatt cactaaagtt
     1801 catcacataa gggagcccat actcatctgt tcctaataga atcactttgg ctaatgaagc
     1861 cagttgttta ttccgcaaag caacgtcttt tatgacaatt gctacctggt acgcttgctg
     1921 atactctgct gtatattttt gggactcctg aaatttattc ttccgacgtt ccttgtattc
     1981 tttactattt agcaaagctg tttgccgttc tttctttgaa acaacttttc ttttttcaat
     2041 aaaagacgca tacgcaatca aacaacttcc tgcacctgtc ccacctaaaa ggagggcaaa
     2101 aatcgtttgt ccattaaact gcaacgcatg agaaaacaag tttaatggaa ataataagta
     2161 acacgacaaa taccctctag gaagaacaaa aataaaacaa aatagattta atagcgtaaa
     2221 acctaacgca aaaataaaaa aaagagagct tcttcgtttt aagaagctct catactcaaa
     2281 gtcataaata tgtgcacgtg tctctttttt atctatcata aagacaatcg ctaaaggaat
     2341 actgcctaca ataaaaaatg gatatagaca aacacctaat aaagtgacga taaaataaag
     2401 ttgttgaaac cgactttttg gcgtttcttt ttcttgtttt cttttgcgat cgacagaaaa
     2461 catttgctca aaatgtgtcg gtcgttttgg tgcattcatc atactatcga tttcctccta
     2521 tcgttttcgt tgtttcattt ctcttcgata aatcggttgt ttttctcgtt ttcgtttcat
     2581 ttttgcttga gttgctcgaa actcactccg tgtcttcggt gcttgttttg gtgattttgt
     2641 ttgtttcatg gttgagtatg ctttagatac ggttgaaggc gctttggttt gtgctgttac
     2701 tcgattgact gctgacatat gttcccgaac cccctctgga cgtcgaacag atttcggtgt
     2761 ggtagaacct gttgctcgtg gtacattccc ttttcttagt ggtttttgta ccgcatgttt
     2821 ttgtcctgca actgtccggg ttttcatcga acttgttttt gttacgggag taagtgcctc
     2881 cttccgacga cgacttgttt gtccctgacg acgttttcga tcgttcaatt ctgttctagg
     2941 cgtatgcttc tggccataag acttgtgttt ttgctttgga ttcgctaatg acaccaccat
     3001 tgcagaaccc tgattgcgat acggactacg agacgcacct tttctacttc tttctcttag
     3061 actaggattg ccttgcaatt ctcgttgtaa taaatcggct gtttttcttg cctgtttctc
     3121 cattgctgtt cgttgcatac gttcttgact ttgctcttct gcttgtttcg cttgatcatg
     3181 caagtctctt gctttttgtg ccatacgttc catacgattt ggtgctttat gacgaggttg
     3241 ttgtgcggat tcacgccgaa aagcacttcg tgtggcttgt ttcgccgttt gtggcatacg
     3301 aacgtctgag ccacccccac gttttcgttg acgttcttgt ctcttccgtg cgttttcttt
     3361 ttctttttgt ttctgtgctt ttctcgcttc tttgcgttct ttcaaccgtt gtttggcttc
     3421 ccttctcaat tttgcttctg ccccaaatgg atttcccgca aaagctaacg ctcctcgaac
     3481 acccattctt ccagtaaata gactgcctaa aaagcctcgg aacaaataaa ttcccaacgg
     3541 cgcaaacaaa taaacaatga tcaagagaat tttttcccat ggatcgtctt gtgtactact
     3601 taattggaac ccgtaactaa gaaaagaaga aaaaaggata catgcaaaac ctgtcattcc
     3661 ttttaaaaac gtcaaaatac caaaaacttt ggcatagtta ccaaacacat tcattgtgac
     3721 aagaaacaat cctaaaaaaa gtaagaacgg caacatcggt actaacaata gttgcatagt
     3781 tccaataatc aatcgaaaca tacttaagac aaaataaaca acggtttgaa tcagattcac
     3841 gacaaaataa aacaaggcaa atgctgtatt tttccaacta ttgtaatatt gtatgttctt
     3901 gtttttcagt gtatctgctt catactctgt gactttgtca tgaattttct cccctatttt
     3961 tgtacctaaa tcattgtcca ataaaagatt gattcgatca tattcttttc cagcatattt
     4021 gactttcttc tcacgaattg tgtctacatt ggttgtgcca taatttaaca acagatatgg
     4081 ttcataaaca ttactataaa agacccgagc cgccatcaat ttgcctgccg tttccacttt
     4141 tttatccgct gttacctctt ctgtttcttt tgtttcataa tttgtttctt cttcaggtgt
     4201 taacactgga ttgaccttca cgaacgccgt ttccactgta gaatctaaag aaaaaagaac
     4261 atcaaaaaga ttcttatttg tcccagaatc ttttaatacg cttaacccag taaaaataag
     4321 gatcgtcata agaaagacac ggagtgcttg tttcattcga gcctctccag catatttgat
     4381 tgccatcata attgccgaaa aactgattcc aatagttcct gcaatactta acatattgtc
     4441 cgcaattttt tgtgttaagt tttgaatggg ttctcgaatg gcggaaataa tatctaattc
     4501 aaagagaaat tcaaccattg aagcattgaa ttctcctaac atttttacgc cactccaaac
     4561 aaaatttttc atcactcctg caacattgta cgatagcttt tttttaaggt tcattgactc
     4621 atagtcattg gtcatcaatt caaacgaatt ctttttgtaa tttttataca attgatccac
     4681 atcagtcgtt acttctttta cttctacttt atcgttggca ttctctcctt cagaacgatc
     4741 ggtttcagtg acacccgcac catcattcat ttgatcggca gaaacagtag ccccttgtag
     4801 acagaaaaaa agacccccta ataaaaggag cgtccaccat ttcagagatt tttttcgcaa
     4861 aaaaaccacc tcttttttgt tttatcgttg tttttcattt tctaacattg cttgaatttt
     4921 ttcatcttga ttttcagtag aagaaacagc caataaccaa ctatcaaaca acacatcaat
     4981 ggcaatcggt tgatttcttc ccaaatgatc ctggaataaa caggttcctg tttgtaattg
     5041 ttccatcatt tctaaattgg ctgaattcac ttccatatca aagaaggaca atagtttctc
     5101 tcgtgcttct ttttgtttcg gacgaaacgc aaatttgtaa gaaattaact cttttttatc
     5161 ttcttgatcg taatctttaa acgcttgcac aacaaggtaa atatccgtat tattcgcacg
     5221 tccttttcgc aagctatcat caatcaaata gcttccttgt gcggtatctt catacccttt
     5281 tgcctcgtca taaataatcg cagcgtcttc ttctttgttt gtagaaaaga tatacgtaaa
     5341 cttcataatg acttccataa ttgccattcc tgcaagttgt tcgctattta atcgacccga
     5401 atcaatctct ttttgtgtcg gaatgcgtat tccttgtgtc cctaacacgt tgatttgtga
     5461 atcaaaacgt atcggcgtac tgtgctcatt cccaatgaga atctttccta aaccaacgct
     5521 atgccctaaa atcaatttag ctagttcttt gtcacttttt cgaatttctt cgataacttg
     5581 acttaacgaa tgcttacctg gcatggtgta aactacatcg atggcgtctt ggatcaccgt
     5641 tttcttgtgg ctcgctgttt ctcgattggt atctacttct cctaacattc ctaacacatt
     5701 tttagcggtt tgaattgcct cttcttttgg taaaaacaat agaggatcaa gcatccctcg
     5761 acagctttca tctgaagata acgaaataaa attaattcgt tcatacatag ccacaaactc
     5821 tggtatgtgt ccgaattttt caagtgcctt taaaaagaac ttttgggttt catttttcgg
     5881 atcaatataa agaatttttt gacctaaaaa catcagccat aaaaaaatat attttacaag
     5941 gaccgatttt cctttacctg gtggaccaat aatcaacgta tttccattgg tatgttccgc
     6001 gccttcaata cttttctttg ttaaattggg ataataccat actagtttac tagatagcgc
     6061 taacgcttct ttcgcagatt taaacttttt tgcaataaca atccgtccta aaggaaatcc
     6121 tttcttattt cctacttctt tctttaagtc caagccataa tctgccacaa aacctgtcgt
     6181 agcgacctgc tcgtaactac gaaaacgttg cgtacttcca attaagcatt gattaaacaa
     6241 cgttagctga tccgctaccg atcgataaaa tttgaaatct gtagactcaa tcgcaaattc
     6301 aatatctttg actcgttctt ccaattcttc ttttgaagaa gcagatacaa ccagcgtgac
     6361 ggtagaataa cacaatcgtt tttctttgcg cttcagctta tcttttacat ccattaaccg
     6421 ttctgtacca aaatttaata catcgtctga atctaaaatg ccgtcaacgg tctcatattc
     6481 tttttcttgt tcataaatcc gtccagccat tttattaatt tttcgttcat cagattttga
     6541 atgctcaaat cgaagtcgaa tatgcgtatc aattggaaaa gataagctat cctgaagatt
     6601 ttgaataaag ctactcccat acatggctaa aggagcgtcc gtttgactta aaaacgtcga
     6661 ataatgcgtt tgattcaact gttcaatcgc aatgtatcct gcttcattcg aaatgacgcc
     6721 ctcgcaaatt tccattggcg taagctgtct ttcaggcaat ttttcttctg atcgatggaa
     6781 aaaataataa taaatttgtc caatctcttg ggcagataag cgctctatcg gcttataatt
     6841 ggacaattcc gtatagagct ccttttctaa tgtttcatac gttggtaaca cactacttat
     6901 aggaactcgt tgtcccgtaa tacgatagat aaacttttta aaaacatgct taagcaattg
     6961 aaaatattcg atcggatcag acactttcat ttctgtagtg aaacgcacaa acagatacgt
     7021 tcgatactcg tttaattgca cttcgtcttt taaaatcttt cctgcctgtt caaagtacac
     7081 tttcccaaga tctgcgcatt ctccctttac caaatctcga atggtttctt caatgtgcgt
     7141 atctaggtta aattgacgtg gaatattgat aaaatgatag tcatattcat catgtccaag
     7201 tatgccttcg ccatcttgaa gatagttctt gaaaaaatca atgtcattta aaggaaatac
     7261 ttgggaagaa agttgatagc ctacccacac atcatcattt ttggtcaaaa caaaatttcg
     7321 gataatggcg tctatcggaa attctaatcg aggaagcttc gtttttttct ttttgaactg
     7381 ccaaatcccg ataataaaga cgaccaaaag aaacaaaaac gccatttact cgactcactc
     7441 cttccatttg tgtagggaat acgtcacttt ttcatggaaa aacggcacat acatttcatg
     7501 ttcaaagcgt tgtttccgtt tctgataata ggacgttcgc ccaattaaat actcgaaaaa
     7561 gtgtttattg tcccatttca ttgaaaacaa tgtccaaacg agaacaccaa tcccaaaaat
     7621 cataatcaac caggactttc gaaacagtgt ctgcaagaaa ctaatttgat tcacatacgc
     7681 aatcaaaaat aggatcaaaa gaatgaagag aaatacccca cctatgacta attgttccat
     7741 gcgaatgcca ttagaaatcc agggaatttt ttgtccttta atcccgtaaa ttttgtgagg
     7801 gtgcttaaac tcctttgtat agttgtattc catgttcact ctacctcctt ttacaaaaaa
     7861 agaagcgacc agaagttcta atcttctgat cgcttttgca gtttgtcttt ttcttatgat
     7921 aattgtatcc agggcacaat catttgcgtt acccccacgt tctcttcact atccttaata
     7981 aagataatgt tctttagcca ttcaccaata tcaacggcat tcgataacaa taagacgagc
     8041 attatcaaaa taaagacgcc aataatggca aacgctgccc gcgcaaagcc ttgggtcgca
     8101 taagcaccaa ccattagaac aatcgcaacg attgccaata gccaaatgac ttgtccagat
     8161 acatcatcaa ataacgattt aagatcaaaa agtttcattt ccattctcct ctaagttcat
     8221 tttattcatt taacgtagta acaaaataac tatcgccact tgtttttact tccattgaaa
     8281 agtttgattg gaactcgttt cctttctcaa aatagaaacg atacttccct tgcaaaaagt
     8341 agtttccttc tttatttttt actgcatgat ccagttgtac attgatcaat tcgccgcctt
     8401 ctaacccttt ttcgttagaa atcaatgcca atttttcatc atttttgaca tataactcaa
     8461 aaaaccgttt cacaaatgct tccagcttga tttcttcgct ttcagtgacc gcttgcccag
     8521 aagaataaaa tcgattttcc tgatagttca cagaattggt attcttactc tcagaaagat
     8581 tcaaaaattg aacgccatca agtactttta aaacattgtt ttgataactg actggaaaag
     8641 aaagttgact gcttttccaa tgttctcctt ctgtaaaagt aacctcataa aataggcgat
     8701 acattgcttg tgtcaaatcg atttcttcta gtttaataaa gcgaacatct ttcgctactc
     8761 gattggtagc cgtcgttaca aagcctagat cagacgcttc caatccttgt gctaaataat
     8821 gattgacgga agcctcccat tcgtttcgtt tttctggcga ataagtaaat aacctttcaa
     8881 taaaatgttg tccctcgtac ttcaattgtt ccgtagaaga caaggtttgg ttgacttcag
     8941 ttaacaatgt ttctggattg acttgttttt tcactgctaa acgcgttaat gtatccactc
     9001 gtccaaagtt taggaggagc acgacaccca ttaacagaaa caaacaaaaa aaagcgcctg
     9061 aaaatacact catggcactt ttttgcgaaa attggatcat tcgatctgac ggttctgttt
     9121 tctttttttg gaacaaaaaa cgtttctttt gtttcttttc cttctgttgt ttcggtggct
     9181 tagtttggaa cgcttgtgga ttaaattttg cattactcat gagaaatgcc ataccgttct
     9241 aacaataatt tcggtatttt gtccacaatc actacttcgt ggacaaccac acttgccccc
     9301 tc
//
LOCUS       DAHUWM010000107         9163 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHUWM010000107 DAHUWM010000000
VERSION     DAHUWM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.108"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            44..2821
                     /locus_tag="NK788_001813"
     CDS             44..2821
                     /locus_tag="NK788_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6770141.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2902..3321
                     /locus_tag="NK788_001814"
     CDS             2902..3321
                     /locus_tag="NK788_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCH6770142.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3333..3803
                     /locus_tag="NK788_001815"
     CDS             3333..3803
                     /locus_tag="NK788_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770143.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3820..4599
                     /locus_tag="NK788_001816"
     CDS             3820..4599
                     /locus_tag="NK788_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770144.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4586..5407
                     /locus_tag="NK788_001817"
     CDS             4586..5407
                     /locus_tag="NK788_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6770145.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5424..6473
                     /locus_tag="NK788_001818"
     CDS             5424..6473
                     /locus_tag="NK788_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6770146.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6486..7490
                     /locus_tag="NK788_001819"
     CDS             6486..7490
                     /locus_tag="NK788_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6770147.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8123..9142
                     /locus_tag="NK788_001820"
     CDS             8123..9142
                     /locus_tag="NK788_001820"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCH6770148.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3052 a         1536 c         1940 g         2635 t
ORIGIN      
        1 attttattga caaaatgaat ccgttttcta tgatggagga ggggtgaata agttgttgaa
       61 gaaggaaatt ttatgttact tagagaacca gacagccttt ttcgatttag aacatatgaa
      121 cgaggtcttt acagcacaaa ggttagctga tcattttggg gtaaaaagaa acacagtcag
      181 tcattatttg aaccagttaa atgatgagca actactagtc aaaatcaatt ctcgaccagt
      241 gatttatttc cacaaagaag cttttgaaaa acaatttttt aagttacgca cgaattttta
      301 tttttctatc gatgatctca aagaggaaca gccttttttc gcgcaaccaa aagacttatt
      361 ctcttttatg atcggatacg acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc
      421 tctttactat ccagatggcg gattaccact tttgattaca ggcgaaagtg gcacaggtaa
      481 aagttattta gttaatttgg tctatcagta ttgtctgctt catgatctgc tagaagattc
      541 tgcaccattc atcactttta attgtgcaca atatgcagat aatcctgaac ttttgactag
      601 caatttgttt ggacatgtta agggagcgta cactggtgca gaagaaaata aaaaaggggc
      661 ttttgaagca gcagatggtg ggatcttgtt tttagacgag gtacatcgct taacaccaga
      721 aggtcaggaa aaactgttca cctatcttga tcaaggtgtg atttatcgta tgggagaacc
      781 taatttgtca cgccggatca aaacacgtct cttttttgct acgactgaag agattactag
      841 tcatttctta acaactttca ttcgaagaat acctatccga atcgaactac ctgcacttaa
      901 tcagcgtagt cgtaatgaac ggctagaact gatttactct ttttttctcg aagaacaaag
      961 aaaaatgggg agccctttgt cagtcagtgg accagctttg tcacttttaa caagtcagaa
     1021 attatccgga aacataggag agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata
     1081 tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc aaaaagaact
     1141 acttgcgcag cctattacta aaaatacagc acagcaagga atctatatta cgcaagacca
     1201 gaccttagag caattaatgg agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt
     1261 tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa
     1321 attaaagcaa gaagtgattc aattatttga ttttttgctg tttgagacca gtagacaaga
     1381 aaaacacgag ctgctggttt attggacaca atctatccga gagacctttc gacaaatgga
     1441 aactgcctat caaatcaaat tcgatggaaa cagtgtctat gccttgagtt actatctcta
     1501 tcagcgaagc actatcaaat ggttaccgga agaacatgat attatccaac taatcaaaga
     1561 attagaacaa caagtttctc agtcttatcc atctagttat cactatgtcc aacgaattct
     1621 ggaattaagt aagatcaaat tagatattga agacacaagt atggatcgga ttttactgac
     1681 gatctatcta aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc
     1741 tgctcacggt tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga
     1801 ggatctattt gaatcatttg atatgccact agatgtcaca ccacagcaga ttgcttcaga
     1861 aatccttgat tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat tagtagacat
     1921 gggatctctg aaagaaatcc atcagttttt taaaaaacag ttatccgtac cgcttttgat
     1981 tttgaataat gtgacgacac ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt
     2041 atcattagaa gagatagcag aagagactgt tcgacagatc cagccagaat ggcgactgct
     2101 ctatccagag gaaaacaaac ccaaagcact cattactact tgcttcactg gaatcggtac
     2161 ggcggtccac ttaagtgaac tgctagaaaa aagcttacct acggcttgtc agttgaaaat
     2221 catcccatat gaatatcagc aattaaaaga taagaaaaac agtgacccat tattttccat
     2281 atacgaagtc gtaggaatga ttggcaccac agatcctgat atcaccaata tcgactatct
     2341 ttcattagaa gacctcatct cgggggaaaa gatgtctgta ctagctgaat ggttggacac
     2401 tactatgaat gcttcagaga aggaaatatt caatcagcgc atcattcgta atttttcatt
     2461 ggaaaaagta ctggattccg tgactattct agatacagag aaggtgatgg gagaaatcga
     2521 tcattttatg agagaattag aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt
     2581 agcgttgtat gtccatgtga gctgtctcat tgaacgattg atccgacaaa tgccaatcga
     2641 aacctatcaa ggattagaga aatggaagca gtgtcaaaaa gaagggttat ctgctattaa
     2701 atctgcgttt agtgtcattg aagagcatta tagtgtcatg atccccaatt ctgagattgc
     2761 ctatatctat gatattttga gtgaagatac agagtttctc tcgatagaag aagaattttg
     2821 aacataagca agttgttgcc actaattttg gcatgcaact tgctttttta gtatgtgtaa
     2881 gtgaaaagag gagatgaaaa agtggataga gaaattatct tagcttccca tggaaaattt
     2941 gcttcaggga tattagattc attagagctg atctatggga aaaatcattc tattactgta
     3001 ttcgattgct atactgatga gaattttgat ttagcggaaa cagtaaggca gctttttatg
     3061 aggtacaaag ataaagaaat cattgttctg acggatcttt ttggcggaag tgtcaacaac
     3121 gaattcttac aatatatcaa tcgggatcat gtctatctcg ttgccggatt gaatttgcca
     3181 ctattgattg agctagtatc tcggttggac gctgaagtag agacaactgt tttgatccag
     3241 caggtgctaa ctgattcaaa aaaaatgatc cagttttgta acgagcggct caatcagaaa
     3301 atagaagaag acgaatttta ggaggaagaa aaatgattgt attgacaaga gtcgaccatc
     3361 ggttattaca cggacaagta gctttttcat ggacacaaac aataggcgca gactgtattt
     3421 tgattgctaa tgatgatgta cctacgaatg agatacgaaa gacaacgatc aaattagcca
     3481 agccacaagg agtcaaacta gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa
     3541 gcggggtgac agataaatat aagttattca tcgtagtgga atcgatcgag gatgcatata
     3601 agctggcaga agcgtatcca gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac
     3661 ctggtactcg cagcatcgga aaagcggtaa atattttacc agaagaggag gaacgcttga
     3721 aacgattaat tgacaaacaa atagaagtag aaatccgtca agtaccagca gataaaaaag
     3781 tggatgtaag agaagttttg taagaaagga agaacctcaa tgattgtaca agcagtctta
     3841 ttaggaattg ttgcatttgt cgcacaatca gaatatgcac tagggacatc gttgctttct
     3901 cgtccgattg tgacggggct gtttacagga atcgtgttag gtgatgtaaa aactgggatc
     3961 attatgggcg caactttaga gttagcattt atcggatcgt tctcagtagg tgcttcgatt
     4021 ccacctgatg ttgtgaccgg tgggatttta gggacggctt ttgctattac agctggagcc
     4081 ggtacagaaa ccgcgctttt actaggtttg ccgattgcta cgttgacatt gatattgaaa
     4141 aatatttact tagggctttt gattccaatt atgaaccata aagcagacaa ttatgcagaa
     4201 gaaggaaatt ataaaggagt cgagcatatg cacttgcttg ccggtatcgg tctttccttg
     4261 atgcttggtg ttgtggtaat ggtttcttat atggtgggaa gcaatacgat cggaaatcta
     4321 ttaaatatga ttcctgactt cgttcaaaag gggttgtcag tagctacagg gttgattcca
     4381 gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc accatatttt
     4441 ttcttaggtt ttgctatagc ttcttactta gagattcctg tgaccggagt agctattttt
     4501 ggtgcgattt tagcagttgt ggtagtcaat attatgaatg tccgtcaact accacttcaa
     4561 acaactgcag gggaggtaga agaagatgaa gaattctgag atcaaaaaca gcaaaataac
     4621 gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa atggagtttt cttggaatta
     4681 tgaacgtcag atgaatatgg cttatgttta tgcaatgatt ccgattttga aaaagttata
     4741 tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat ctagagtttt ttaacacgac
     4801 cccacatatc gttacactga ttttaggaat cacggctgca atggaggaag aaaataagga
     4861 tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa acatctttga tgggacctct
     4921 tgcagggtta ggagattctt tcttttgggg aactttgcga ttgattgcca caggtgtggg
     4981 tacctcttta gccatgcaag gaaatatttt aggccctatc ttgttcattt tgattttcaa
     5041 tattccgcac attctgtttc gctatctagc aacaggttgg ggatatcgct taggaacggg
     5101 ttttttgaaa aagatccagg caaacggtat gatggaaagt ttgacattag gcgcgtctat
     5161 tattggactg atggtcgttg gagcaatgac tgccacgatg atcaccatca atattccttt
     5221 aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt atttttgatg atatcgttcc
     5281 aaatatctta tgtcttggag catttggcat agtgttttat ctattgaaga aagaagtgaa
     5341 accattaacg atcttgatcg gactagcttt ctttggaatt tttggttcgt ggatagggat
     5401 tttttaaaaa aggagcagag aaaatgttta cgatgcaaga ttatatctat gaagaaaagg
     5461 aagtactaag tacgatcttg aaaaagaatg atttttcgac aagagaacat atgaaaaaaa
     5521 cagtgaattt actgatctta gcaacgggtt catcttataa cgcttgttta gcagcgaagc
     5581 cagcattgga atcttatggt gatcttactg tcgatattca agaacctttt tattttttca
     5641 attatggaaa actttctcct tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa
     5701 gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca agggttacct gttgttgcta
     5761 tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc acaaattatt gatttaggtg
     5821 ccggggtaga atcagtaggc ttcgttacaa aaggctattc tgcgactgtt ttacagttac
     5881 tactcttagg gataggaatt ggaataagta aaaataaaat cagcaaaaaa atagaacaag
     5941 actacatgca acagttgcgc aaaataatca accatttacc agcgattatt caaaaaacgg
     6001 aagaattttt tgatggatat caaagtttat ttcgtttagc acagcgtttc gtcaccattg
     6061 gatatggtcc aaactgggga acagctaaag aagcagagac gaaattgact gaaacaatcc
     6121 gcgttccgtc acaaggattt gagttagaag cttatatgca cggcccatac ctggaggcag
     6181 atgcttcgca tttgctattc tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa
     6241 agttgcaaag atatatgagt cggtacgttg gagagactct caccattacc acaaaaaaag
     6301 caagaaacga gaaaacactt ggattagcaa tagaatgtga tgaatatctg tctgtattag
     6361 cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa ggtattgact
     6421 tgaacaaaaa gatttttgaa gactttgata cagtcttgaa aagtaaatta taagaggtgg
     6481 aaatcatgct gaaattcaat gaagaagaac aaatcaaagc aataaaaggc gcactagctt
     6541 tacgtccgca agtagaagaa atcatcgata aactttatgc ggaaaaattt gacgctgtct
     6601 attatctagg aatcggtgga acgtatgctt cctcgatgca agctgtcact tatatgaatg
     6661 ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga atattacact actggaaaca
     6721 aaagattaac aaaagattct atcgttattt tgtcctctgt taccggtaca actcaagaag
     6781 tagtgaaagc agtagaacaa atcaagaaag taggagcgac actgattgga tttatcgata
     6841 aagcgaacag taagttgtct cagctatgcg attttgtggt cacttatcca gctcctggaa
     6901 cagaacaaat caaatttttt atggcagcag atcgattgat gtatttgaat ggagaattcg
     6961 aagcttattc agaatactat gaacagctcg aacgttattt accaacaggg ttagtggaag
     7021 cagagaaaaa agcagatgca tttggcttat catttgcaga aaaacatcga cgtgatagta
     7081 tgcattattt tattggagca gggaatcaat ggggagctgt ttattcatat gccatgtgtt
     7141 actgggaaga acaaagctgg cttccatcga aatctatcca tgctgccgag tttcttcacg
     7201 ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt atttttaggc gaagatgaac
     7261 agagagtgtt atctgaacgt gtggctaaac ttttgccaaa aatttgcagc aattatacat
     7321 tgatagacac aaaagactat ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt
     7381 tgtctttctt attgatgcat gtagtgacac aacgcatcga tgctcatgtg gagcagttga
     7441 actgccatcc attagagata cgccgctact atcgtcagtt tgattattag gatagactgg
     7501 ataaaaagaa tataaaaaat aaagagagca gcgataactg ctccctttat ttttttaaaa
     7561 tattggcatc aattacgtta taaaacacat tttttgcatt atctacagtt caacttataa
     7621 tctgtttaat gggacatgtg ggtgccaacc attttttgtt atgcaatagt actatttagt
     7681 tgttgcatgg atccgtagta aaagtccaag tacaacgagt tgcgccagtt tacatagcgt
     7741 tttgtatcca gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa cagagacaat
     7801 ctgtccgttg caggacctga cagatttaga aatgttcaat ctgtctctta tgtattttaa
     7861 atcaatactc actaggagaa gtttcctaac ctcctaatag taatgctggt agtaacattg
     7921 tcaaaactgc ttcctcttag gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga
     7981 ctattgtaac atatcattga ttatttcaag taaaaacgat aaaagaagta aacaaacact
     8041 taaaaacaat ggaaattagt cccaacttta agaaatgtat actataatgg aaattatcaa
     8101 ttatttattt ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg
     8161 gggcactgct ttagcacaaa cattggcaga aaacgggcat gatgtccgta tttggggaaa
     8221 tgtacctgaa cagattgatg aaatcaataa gtatcataca aatcagcatt ttttacctga
     8281 tttgacgatc ccagaatcga ttatcggata taaagaattg gcagaagcag tggataacgc
     8341 agatgctatt ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac aggaacttat
     8401 tggtaaaatg aatactaagc caatcattat acatgcaagt aaaggactag aacaagatac
     8461 gcataaacga atctcagaag ttttggctga agaaattcca gaagaaaaaa gacaggcaat
     8521 tgttgtatta tctggtccta gccatgctga ggaagtggca gtacatgata tcacaacaat
     8581 tacagctgca agtattgatc aaaaagcagc agcctatgta caaaaactgt tcatgaatga
     8641 ttatttcagg atttatacaa ataatgatgt gattggcgta gaaactggtg cagcactgaa
     8701 aaatatcatt gcaattggtg ccggcgctat ccatggatta ggattcggag atgatgcaaa
     8761 agcagcaatc atgactcgag gattggcaga aatcagccgt cttggtgtcg cgatgggtgc
     8821 caatcctctg acatttatcg gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag
     8881 tgttcattct cgcaattggc gtgccggtaa cttgttagga cagggacata agctggaaga
     8941 agttctagaa aacatgggaa tggtcgttga gggtgtggca acgacaaaag cagcagtaga
     9001 gctagctcaa cagctaggtg ttgagatgcc aattactcaa acaatctata atgttttgta
     9061 taatggagaa gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga
     9121 gaatgaattt tctcttcgtt aaatagatga tcaatagtta atc
//
LOCUS       DAHUWM010000108         9101 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHUWM010000108 DAHUWM010000000
VERSION     DAHUWM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.109"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..1271)
                     /locus_tag="NK788_001821"
     CDS             complement(<1..1271)
                     /locus_tag="NK788_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770149.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKC"
     gene            complement(1369..1644)
                     /locus_tag="NK788_001822"
     CDS             complement(1369..1644)
                     /locus_tag="NK788_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770150.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            1876..2610
                     /locus_tag="NK788_001823"
     CDS             1876..2610
                     /locus_tag="NK788_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770151.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            2649..3791
                     /locus_tag="NK788_001824"
     CDS             2649..3791
                     /locus_tag="NK788_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6770152.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(3886..5277)
                     /gene="sufB"
                     /locus_tag="NK788_001825"
     CDS             complement(3886..5277)
                     /gene="sufB"
                     /locus_tag="NK788_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCH6770153.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5282..5752)
                     /locus_tag="NK788_001826"
     CDS             complement(5282..5752)
                     /locus_tag="NK788_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCH6770154.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(5739..6974)
                     /locus_tag="NK788_001827"
     CDS             complement(5739..6974)
                     /locus_tag="NK788_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCH6770155.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(6971..8257)
                     /gene="sufD"
                     /locus_tag="NK788_001828"
     CDS             complement(6971..8257)
                     /gene="sufD"
                     /locus_tag="NK788_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCH6770156.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(8270..9043)
                     /gene="sufC"
                     /locus_tag="NK788_001829"
     CDS             complement(8270..9043)
                     /gene="sufC"
                     /locus_tag="NK788_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCH6770157.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         2660 a         1854 c         1628 g         2959 t
ORIGIN      
        1 ttacatttat ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt
       61 tcccccgaca aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa
      121 agcaagacat catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt
      181 ttgtctttca ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga
      241 aaatgtgtag gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg
      301 tctttcttta aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg
      361 gaatgacaat aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta
      421 tgatcgttgt gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca
      481 atggttaaca atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag
      541 tctttaaacc actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg
      601 gtttcattga ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc
      661 atggtaaaat caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc
      721 actgcatctg acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca
      781 cacccgggga tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta
      841 attgcttctt ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata
      901 cttttttgta tcgcaaacgc agaattatga actaaattga tagataaaac cttttctacg
      961 cttaagtatc tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca
     1021 gtgttatcga catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct
     1081 ttattctttg tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca
     1141 tctcttgtaa cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt
     1201 tttgtaactg gcttctctaa taatttttct tgatctacat aaattggaat attaatcatt
     1261 gatgtttcca tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata
     1321 ataaaaacaa actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata
     1381 ccgttccatg aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat
     1441 aacaggtaac ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata
     1501 ttcacttgcc tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag
     1561 cataggcttt agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat
     1621 gctatcaatg atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt
     1681 tctaagaatt atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta
     1741 atcaaacaaa aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg
     1801 gtgtcaacag ctttgtaaaa tttatttgca acaccccttt tttgaggtat actaatttta
     1861 ggaggtgtgg tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata
     1921 ctcggaacaa aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt
     1981 cttatgatta acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga
     2041 acctatttac caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata
     2101 gaggagttag tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat
     2161 agcattgtac tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa
     2221 cagttcccac cagtacactc taatttagat acatggctgg atagatattc caaattagaa
     2281 ccagagatgc aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt
     2341 agtcttttca atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt
     2401 gacaataaca ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat
     2461 gaatggctac ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt
     2521 actgataata taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag
     2581 aaaaaaatga agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag
     2641 atgatttaat ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt
     2701 ataaaggaca cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag
     2761 gttttagtag caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat
     2821 acggtgttaa aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa
     2881 ctcatcagcg taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata
     2941 atcaaatact tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc
     3001 aaaaagtggt agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga
     3061 ctgcacaaat tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa
     3121 aaacacttct tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg
     3181 agcttaataa tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct
     3241 tcagattatt ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag
     3301 acatagacac gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg
     3361 gtaaagttat aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata
     3421 ctgagactct ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac
     3481 ttggatacaa tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat
     3541 acattccaaa cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc
     3601 gtattacacc tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat
     3661 ctgtcaaggt agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct
     3721 atgcacatat tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg
     3781 ctcatcagta aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa
     3841 agctagaacc cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc
     3901 ttccatctca taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt
     3961 ggtgaatggt tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc
     4021 tcggctcatc agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc
     4081 taaagagact tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc
     4141 gtccatgatg atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa
     4201 ggttacttgt cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat
     4261 agagctagat gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt
     4321 tgctcctgca aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac
     4381 acttgggtat ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc
     4441 gccttcgtat gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg
     4501 gatcgttgtg taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg
     4561 caggctgtta cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc
     4621 ttcgtcaaca atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa
     4681 gtacgtttgt aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc
     4741 tgaccatacg gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga
     4801 aaagtactct ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac
     4861 gattcctaac ttttcgaatt catctttcat attatggtac acgacttctg attcatattg
     4921 tgcggaagca ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt
     4981 atctttgatt ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg
     5041 gtaatatttg atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg
     5101 cattttgtta aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc
     5161 tttgattctt gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac
     5221 cggttcgaca tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt
     5281 ctcactcctc ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt
     5341 ttcctagtgc tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac
     5401 caccaagcat agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa
     5461 gctgtagaaa gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt
     5521 ctgtcatcat cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat
     5581 ttttgatgat tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt
     5641 tgttcaattc gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag
     5701 aatgatccaa gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa
     5761 aactcctttg ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag
     5821 aaataaaagc tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca
     5881 caatgatgac ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg
     5941 ggatgtaacc catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga
     6001 ccataaacag tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct
     6061 tcttcatatc gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct
     6121 aatgcgatag cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc
     6181 catgtactgt cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc
     6241 atttcttcta accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt
     6301 ttgtgtccgc tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc
     6361 ggggcagatt gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct
     6421 aattcttgaa caggattgaa gacacctaag acattggata catgagcaat ggagacgatt
     6481 ttggttttgt ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt
     6541 agttcgatat atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca
     6601 ttagaatgat gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg
     6661 ccgaaggttc tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct
     6721 gctgtttctt ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg
     6781 gtcgcgcgtt ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa
     6841 tagtacgcta atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga
     6901 taaactaaag gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt
     6961 ttagcatcga tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt
     7021 gttctacagg gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg
     7081 cttcttcttt atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc
     7141 ctacactggc agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg
     7201 catcaccacg tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac
     7261 cttttgcccc tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga
     7321 tcactccgtg ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat
     7381 cgattccttg gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac
     7441 cttctcctgc taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa
     7501 ttgcccagtc gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa
     7561 tattttctcc taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga
     7621 cttccacgat gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt
     7681 ccagataaga aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct
     7741 catcggatgc gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt
     7801 taggcacata caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt
     7861 cttcgttcat gtttactgct tttttcatat aatattcttg gactagttct ggatattcga
     7921 tcattgcagt gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt
     7981 gttcaaaaat cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa
     8041 atgccggaat cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc
     8101 agcgatggaa tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg
     8161 cgtcttgacg gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta
     8221 ccgcgtcaag ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt
     8281 cttctttata gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac
     8341 gtttagctag gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga
     8401 catcaggtgt gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat
     8461 tgtctccgcg catttcattt actcctttag caacgacttt caaagcatcg atatccagac
     8521 ctgaatcgat ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct
     8581 cattccgttt cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt
     8641 ctggcatatt tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa
     8701 ttttgtcgtt ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga
     8761 tacctggtat ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat
     8821 ctacttccaa gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat
     8881 agtttggatt gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca
     8941 tgatggcatg gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt
     9001 cctcgatcga cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat
     9061 tctctttcgt tctcactcat tttagactaa ttgagaatga a
//
LOCUS       DAHUWM010000109         8895 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHUWM010000109 DAHUWM010000000
VERSION     DAHUWM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.110"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(14..1243)
                     /gene="hflX"
                     /locus_tag="NK788_001830"
     CDS             complement(14..1243)
                     /gene="hflX"
                     /locus_tag="NK788_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCH6770158.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1245..2162)
                     /gene="miaA"
                     /locus_tag="NK788_001831"
     CDS             complement(1245..2162)
                     /gene="miaA"
                     /locus_tag="NK788_001831"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCH6770159.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2166..2903)
                     /locus_tag="NK788_001832"
     CDS             complement(2166..2903)
                     /locus_tag="NK788_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCH6770160.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2994..3521)
                     /locus_tag="NK788_001833"
     CDS             complement(2994..3521)
                     /locus_tag="NK788_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770161.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3701..4294
                     /gene="clpP"
                     /locus_tag="NK788_001834"
     CDS             3701..4294
                     /gene="clpP"
                     /locus_tag="NK788_001834"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCH6770162.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4398..4883)
                     /locus_tag="NK788_001835"
     CDS             complement(4398..4883)
                     /locus_tag="NK788_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCH6770163.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5036..5233)
                     /locus_tag="NK788_001836"
     CDS             complement(5036..5233)
                     /locus_tag="NK788_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770164.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5328..7088)
                     /gene="efrB"
                     /locus_tag="NK788_001837"
     CDS             complement(5328..7088)
                     /gene="efrB"
                     /locus_tag="NK788_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCH6770165.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7085..8815)
                     /gene="efrA"
                     /locus_tag="NK788_001838"
     CDS             complement(7085..8815)
                     /gene="efrA"
                     /locus_tag="NK788_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCH6770166.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2510 a         1979 c         1490 g         2916 t
ORIGIN      
        1 aaaaaataaa attttattgg ttttttctta accattttga attggttttt gcaaaccctt
       61 taacttcata ttcgtttgtt tcttccatgt acgcttcgtc caaaaccatt gtttgtcttc
      121 tcaactcgct taatttctgt ccttcgttgc ttggcacaga aagtgtataa ggggtcatta
      181 gttccatcag ctgacgtttg attgcttcga tcaacgccat cttaccttct ttcgtctgag
      241 tagaaatcaa tacatttggg aatagcgtag gcgtgaacat cgctgggtcg atctggtctg
      301 ccttattgta aacagtcagt atcggcattt ctttcatatt cagttcatcc atcaatttta
      361 agacagtctg ctcctgtaaa atacgatctg gagaactagc atcgactaca tgtagcaaaa
      421 gatccatatt ttgactttct tctaatgtag aatggaaagc atcgatcagt tgcgtaggca
      481 ggtcttggat gaacccgacg gtatccgtta cagtaatctc aaatccttca gcaaaacgcc
      541 atctcttagt caatggatct aacgttgcaa acaattgatc tttcgaataa gtatccgctt
      601 gtgtcaatag attcaagata gttgacttcc cggcattcgt gtagccaata agtccaatct
      661 gaaaaatctc gctatttttt cttttttggc gattgcgctc tcgatgagct tctacttctt
      721 tcaattcttt cttaacagca aaaattttat tccgtatatg tcggcggtct gtttctaact
      781 ttgtttcacc tggccctctt gttccgatcc caccgccaag acgagataat gttttccctt
      841 gccctactaa acgaggcagc aggtattcta actgagcaag ttcaacctgc agcttccctt
      901 ctttcgaacg agcacgcatc gcaaaaatat ctaagataag ctgcacgcga tcaattaccg
      961 gaagaccaac agcatcggta atcagttggc tctgtctcgg agttagctca tgattaaaga
     1021 tgatcaaatc cgcttcatgc gcatccgctt gttgaactaa ttcttgtaat ttccctttac
     1081 cgataatcgt ttgccgatcc acttgtggtc tcttttgtgt aattgtgaat acgacttctc
     1141 ctgatgcggt attcgtcagc tgaactagct cttccattga ctctgaaaag taacgctggt
     1201 ttgcttcggt ttctacccct acgatgatca ctttttcttc cattttattc cgcctccagc
     1261 cattttttga ttgtttgctc aatctgactt ttttgatttg gctcacttat aagatcaaac
     1321 cactgtgcat ccaaacggtt acgaaaccac gtcaactgac gtttcgcgta tcttctcgaa
     1381 tgtagtttga cttgttctgt tgcttcttcc aatgtttctt tcccttcaaa ataagggaaa
     1441 aattctttat atccaatacc ttgactggcc tggacttctc caagctcata caccttacgc
     1501 gcttctgcta ctaaaccttc tgctagcatc aaatcgactc tttgattgat tcgttcataa
     1561 agaatactac gcttcgtatc taatccaatc atgaggtaat catacaattg ctttggctct
     1621 tctttaggtg ccgtgatact atgtcctgtc gtctctaaaa cttccagagc tcttatcact
     1681 ttgcgttgat tgttccagtg gattttctca gcagctgccg ggtctttttc tttcaactga
     1741 ttccataatg cttgggaacc aattgtttca gctaacgttt catacttttt tctaatctca
     1801 ggattcgctt cttctgtcgc tcctaactga taatcatata gaagggattg gatatagaga
     1861 cccgtaccac cagctatgat aggtaggtgc cctcggttcg caatctcggt aatcaattgt
     1921 cgacctgaat gctgaaaatc agcggctgag taacgttgag taggttcgat cacatcaatc
     1981 aaatgatgcg gaacattatt catctcttct tctttgattt ttgctgtacc gatatccaga
     2041 tttttataga tttgcaatga atcaccactg atgatctctc ctccaaatag ttgtgcgagt
     2101 tccacactta gcgctgtttt accgactgct gtcggaccga cgatcactaa tactttttcc
     2161 atattttacc tacttttcgt tgattgtttc caccgaagag ctttctccgg aaaatcagtt
     2221 attattccat ctattttgag ttcttgacat ttggccattt cttcttcatc gttaatcgtc
     2281 catgcacgaa ttgcttttgg aaaatcagct gcctggactg gatgttcgaa caaccaagta
     2341 ttttttggat gtactccttc tatataaggg gtttctgctg ctagttggat ctttttatca
     2401 gaagtactca tcaataagtc cagctgtatc tccggttcca gtaatgcgat tttttccaaa
     2461 gaatccaaat gaaagctgca atatgcatgt tgaaaaggcc aatctctact ttttaatgtt
     2521 tcggctaatt tttcttcgat accgggatat tcatactgat cagttttaat ttcaatcatt
     2581 agcatccctc ggtaattttt ttggaggagc agtcctaaca cttctctcaa ttcaggaact
     2641 ttagctgctg gaaattcttt gctgaatttg ccccctgcat tcaatttttt gagttcttcc
     2701 aatgtttttt ctcgaatcag ccctttaccg ttagtcgtcc ggtccacttc ttcgtcgtgc
     2761 ataacgatca aataaccatc tttgcttaga tggacatcta gttcgatacc atctgcacct
     2821 actcgtacag cttctgcaaa tgcaggaagc gtattttctg ggtgggtgcc gctgcttccg
     2881 cgatgggcat agatttgtaa catctttctt cactccgttc agttctattg tatcattaag
     2941 aaaaaaagaa agcattacca cgctctcatg tgcaacggca atgcttcaaa atatcagctc
     3001 caaacatcat ctttcgagaa gaaaccaatc ggctggcgcg tcatagtctt caactgagct
     3061 tttttttcat tgatcatttc agtcagacta tccgtaaaat tctctaaaat cacgcgtccg
     3121 cctttataag catatccacc aatcttcatt gtttcatctg gagataaaat cacttttcga
     3181 tgggagatat tcttaaatag ctcgtctaaa atataatttg gcagataaaa actattttgc
     3241 gctgaaaatg attggagata cgcaaaatcg tcaatcgtga tataacaatc atcaaatgca
     3301 tcaaagctga catattcttc catcagcgtt gtccgttttt gcaagatctc gttgatcttt
     3361 ttatttaatt gattcagcgt ctcgatttct ttgactttca attccaattc acgtctacgc
     3421 tgttctttat tttccaagtg acgcatttgc agtatcatgc cgatatagac aactacagca
     3481 cctgtcagca ctccgataag gccagtaata ccaactatca atctgttgtt cctccttgtt
     3541 ttgtttctct gcttatcata ccataaagcg ggtttatgcg actaatttgc ctaagttttt
     3601 tatttgacct tttttgacca aaaatgtata ctacatttta ggtattagaa actaaattat
     3661 ggtacactat ttctaaacga aatcaaggag gaactctatt atgaatttaa ttcctacagt
     3721 cattgaacaa tcttcacgag gtgaaagagc ttacgatatc tactcacgtt tattgaaaga
     3781 ccgtatcatc atgttaagcg gccaagtaac agatgatctt gctaattcga tcatcgccca
     3841 attgcttttc ttagacgctc aagattctga aaaagatatc tatctttata tcaattctcc
     3901 tggtggttca gttacagcag gtatggctat ttacgacaca atgaactttg tcaaagcaga
     3961 tgtccaaaca attgttatgg gtatggctgc ttcaatggga agcttcttgc tgacagctgg
     4021 tacaaaaggt aaacgatttg ctttaccaaa tgctgagatc atgatccatc aacctcttgg
     4081 cggcgctcaa gggcaagcaa ctgaaatcga aattgcggca cgccatattt tgcaaacacg
     4141 cgaacgctta aacaaaattt tagctgaacg cactggacaa ccgcttgaag tgattgaaaa
     4201 agatacagat cgtgacaact atatgacagc tgaacaagca aaagcatatg gattgatcga
     4261 tgaagtcatg gaaaacagtt cttcattaaa ttaatctgtt tcaaaggaag cttttttaag
     4321 ctctcttatc taaaccaaat aaaaaaggat ggtcaactca aactgaccat ccttttttat
     4381 tttatggatg ctactgccta tctttgctcc ttgaattttc cttgcgcatg catccgacct
     4441 atctcgacat aatgctggat gttttctttg tttttttgta tttcttcttt tgtcaaagga
     4501 cggatcactt tagctgggcg accaaaagcc aagacattag gaggaataac ttttccttct
     4561 gttactaatg atccagcacc aatcaaacta ttctcaccaa taaccgcatg attcaatatc
     4621 gtggtcccca tcccgatcag cgcccctttt tcgattgtac agccatgtag catgcactga
     4681 tgtcctaccg tcacattctc cgcaatatct acaggtgcat catgatcgac atggatgatt
     4741 gtaccatctt ggatattcgt tctttgtcct atttttatcc aattactgtc cccgcgtaaa
     4801 acagcttgat accagatcgt cacatcttct gacaatgtca catcaccaat cactgtggca
     4861 tttgatgcta taaaccgttc catatttatt tttctccttt cattttttgt agaaactaat
     4921 cctattgaat aaaggtatca tagcatatat ttatttcata agaaaaaaaa caggtaaaac
     4981 ctgctcaggc cagatgtcta tttacagtat ctggtaactg acgttttacc tgtttttatt
     5041 gtacatctat attttcttct attacttttt ttaaaattaa ttcgtacact tctatttgtg
     5101 ctgccaagcg ttcgatttga aactcatttt caaaaaaatc agctgaatca aaaggcttcg
     5161 tctgacagac gtgcgttaaa cgatcaactt ctcttttggc ttcagtaatt ttttcattta
     5221 aaaactgttc catctccgtg tcctcacttt tcttgataac tttactttac cattatcaag
     5281 aatagagaac aaaaaataag ttttaaactg ttttattctt atatgactta ttctgcttcc
     5341 tcagcaaatt ggctgttata cagtttttca taaaatccac cttgtgccaa taaactctca
     5401 tgcgttcctt tttcaatgat ctgaccttga tccatcacta gaatcagatc tgcatcacgg
     5461 attgtcgata aacggtgagc gatgacgaaa cttgtccgtc cctccatcac gcgatccatt
     5521 gctttttgaa tcaacgcttc taaacgggtg tctactgaac ttgttgcttc atctaaaatc
     5581 aagattttag gatcggagac cactgctcgg gcaatcgtca gcaactgttt ttgtcctaaa
     5641 gacacattgt ctccctcaga attgatggcc atatcatatc catctggcat cgttcggata
     5701 aaatgatcta cgtttgctgt tttcgcagca tcaactactt cataatctgt cgcatcaagc
     5761 tttccgaagc ggatattatc tgcaatcgtt ccttgataaa gccatgcatc ttgcaagacc
     5821 ataccaaata gcgaacgcac atcactgcga ctcattgatt tggtgtcgat tccatcaatc
     5881 cttattgctc ctttgtctac gtcataaaaa cgcatcaata gattgatcaa tgtcgtcttg
     5941 ccggcaccgg tcggaccgac cacagctacg gtttgaccag ctttcacttc aaaattcaaa
     6001 tcttggatta atggtttctt cggatcatat gcaaaatcga catgatcgaa tgtcacatct
     6061 cctaaaatcg tttctggcag tgaagcatct actttgtttt ctttttcttc tggctcatct
     6121 aaaatctcga atacacgttt cgttgccgct gatgcacttt gcaaaactga agacaattgt
     6181 gtcacgttcc ccattggctg actgatttgc caaatatatt ggatgaatgc ttgtagttga
     6241 ccgacaacga tcgccccagt aataacataa aagcttccta gaacagccat accaatataa
     6301 gtagcatagg cagttaattg aactagaggc atcatcaagc cagaaatgaa tgctgcacga
     6361 aacccaaagt atttcaagct gtgattaact tttttgaatc cttccaatgt ttctttttcg
     6421 cgtccatata ctttcaatac actaaaacct gtcatatttt cttggacgta gccgtttagc
     6481 tcaccaagag aattctgcat gctttgaaaa tatttctgcg aagcattgat cactcgtttt
     6541 gagatccaga tcgatgttgg aatcatgatc atagagatca gcgccatcat cggctggata
     6601 taaaacatca ttgctgcagc caacgtaatt cccaatatcg catttacgat accaataaaa
     6661 gcttgttgta aagcaccgct gactgcatct acatcattcg tcacacgaga caaaatattt
     6721 ccttgctggt ttttatcgaa atatgaaacc ggaagacgat tgattttttc ttctatgtct
     6781 cttctcaaat catgcatggc actttgaacg acatttgtca tcaaaacacc agaaagtaat
     6841 tgcgaaatac aataccctat tccaacaact gtgatccaaa tgagacattt gaatatataa
     6901 gaaaaattga ttgattcgcc attggcaata ttacggctga tttctgtagt aggaagtcct
     6961 gtaacatatg gcaaagccac gttgaaagcc actgtcagaa ctgtaaatag gatcactaaa
     7021 agaaaagtct cacgatacgg tctgatatat cgagctaaac gttttaagga agaaaaggtt
     7081 tttttcatgc taattcctcc tttgataatt gagaagcggc aatatcataa taaatcgggc
     7141 atttttccaa caactcccga tgcgtcccca taccgacgac ttctccttca tttaatacga
     7201 tgatcttatc tgcatgcatg atcgttccca cacgttgtgc tacgatcaat acagtagatt
     7261 ctgtcgtttc ttttttcaga cgagcacgta acttcgcatc cgtttgataa tctaatgcag
     7321 aaaagctgtc atcaaaaata tagatttccg gacggcgtat gatggctctg gctattgcca
     7381 atcgttgttt ttgtccacct gaaaaattgg aaccaccttc tgataaatgt tcattaaatc
     7441 catctggttt tcgagaaatg aattctgaag cttgagcaat ctcagctgca cgttccattt
     7501 cttcttgcgt agcatcttct ttcccgtatc gcaaattatc tgcaatcgtt ccagtaaata
     7561 gcagcgcttt ttgcggaata aacccaatct tatctcgcaa cttgcttagc cgataatctc
     7621 tcacgtctac tccatcgatc aagatctttc cttcactcac atcataaaag cgagggatca
     7681 attggatcaa cgtagatttt ccgctaccgg tacttccaat aaaagcaacg gtctctcctg
     7741 gagaggctgt aaaactgaca tttcgtatca ctggctcttg cgaatggcct ggataagcaa
     7801 atgttacatt ttgaaattcg atataccctt tagtttttgt atcagatata ccatttggat
     7861 cttctacaat cgaaggtttg gcatctaggg cttcttggat acgtccagct gaaacagctg
     7921 ctcttggata catcatgaac acactagcaa ataacatgaa tgagaacaag gcatggaata
     7981 tatactcgat aaacgcaatc aaatctccta cacgaagctc gcctgcatcg atttgcactg
     8041 ccccgctcca gatgatcaat accattacga tattaaataa gaaataaaaa cccggttgtg
     8101 caacagccat caaacggaat aagctttttg aactagaagc ataattctca ttgactacag
     8161 caaaacgttt ttcttcaaac ttttcattga cgaatgcacg aatcacacgc aatcctgaaa
     8221 gattttcccg caagatactg ttgattttat ctaagttttt ttgttgcttt ttagataacg
     8281 gttcagacac cttagcaatc gctacgactg ccaataatag taaaggtaat gcacctacta
     8341 catacaaact gagtgtcaca cttgtacgca tgaccatgta caaactgaca atgaacatca
     8401 ttggtgccat aaaaccgata cgtaaaatat tttgcaggaa caacatgatc tgataagcat
     8461 cgtttgttgt acgagtgatc aatgaagata cgcctagatt ttcgtattcc tgatgcgaat
     8521 aactttggat cttttcgaac aaatcatctc gaatatctgc tacgatatta gtcgtaatcc
     8581 tcgaaccaaa ataacctaac aagatattca aaataatacc gattacagta ataacgatca
     8641 taaatatccc ttgttgcttc acataatcaa aatcatcatt gcggattcct acatcgatca
     8701 tacgtgctaa aatcgtagga agtcctaatt caatcaaaat aaaaccaaat acgcatatga
     8761 aattgaaaaa caagaatttc ttataacgca tggtataatg ccacattagt ttcactttct
     8821 catctccttt taatcttttg ttacattttc attactaaaa atgatagcac aacgagccta
     8881 ttaatagaac acttc
//
LOCUS       DAHUWM010000110         8850 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHUWM010000110 DAHUWM010000000
VERSION     DAHUWM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.111"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            148..1209
                     /locus_tag="NK788_001839"
     CDS             148..1209
                     /locus_tag="NK788_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCH6770167.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1212..2108
                     /gene="murQ"
                     /locus_tag="NK788_001840"
     CDS             1212..2108
                     /gene="murQ"
                     /locus_tag="NK788_001840"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCH6770168.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2122..4104
                     /locus_tag="NK788_001841"
     CDS             2122..4104
                     /locus_tag="NK788_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6770169.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4169..4615)
                     /gene="nrdI"
                     /locus_tag="NK788_001842"
     CDS             complement(4169..4615)
                     /gene="nrdI"
                     /locus_tag="NK788_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCH6770170.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4826..6250
                     /locus_tag="NK788_001843"
     CDS             4826..6250
                     /locus_tag="NK788_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6770171.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6431..7204)
                     /locus_tag="NK788_001844"
     CDS             complement(6431..7204)
                     /locus_tag="NK788_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCH6770172.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7201..8106)
                     /locus_tag="NK788_001845"
     CDS             complement(7201..8106)
                     /locus_tag="NK788_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6770173.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8260..8580)
                     /locus_tag="NK788_001846"
     CDS             complement(8260..8580)
                     /locus_tag="NK788_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCH6770174.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2783 a         1582 c         1879 g         2606 t
ORIGIN      
        1 tttaggttat gacaattctt ttgataatat aaactcaacc tttagaaatg aaatttaatt
       61 attttaataa aagtattgaa attttgtttc tttagatata tactctagtc atagaaatga
      121 agacggatac agaaaaagag ggataatatg tttggctttt caatatttat gaacgattct
      181 ttgtcagaag agaagaaaca gtatatccgc caaatggcat ccaatggctt tgtcggtatt
      241 ttcacttcca tgcatatacc agaagatgat gtttcggcat atcaaaagcg attgatcgaa
      301 ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg tcgatatttc aggagaggca
      361 ttggaacgag cgggtttttc attagaagat ctaacgcctt tgaaagcaat tggtgtaaca
      421 ggtttaagaa tggattatca tatttccaat cagcagattg ccgactggtc acatcaattg
      481 aaaatcagtt tgaatgctag cacgatcacg cctaaagata tcgatgaatt gaaagaagca
      541 gaagctgatt tctcacagat tgaagcttgg cataattatt atccacgtcc cgaaacaggg
      601 ctggacaaag aatggtatca gaaaaaaaat caatggctca aaattcaagg tctccttgtc
      661 caaggttttg taccaggaga tacagagttg agaggcccac tatatcaagg attgccaact
      721 ttggaagaac atcgagggat ccatcctctt gcaggcgctc tagatctatt agcaagtaac
      781 acggatattg tttacatcgg agatgcaggt ctgtcagaaa acgttcagga gcaatttgct
      841 tctttccaaa aagaacagac agtcttgctc catacggaac ctgtagatga agaattttac
      901 gaatatatat tagggaagca tacgaatcgg caagacgatg caagagatgt catacgaagc
      961 gtagatgcac gttttcgaga gattccgcct atcccagcaa gaaatacagc tacgcgtatg
     1021 aaagggtcta ttactttaga taacgaaaag tatttgcgct atatgggtga gatccaatta
     1081 accaaatatg atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa
     1141 gagctaccat tgatcaacca aatcaaagct ggtatgaact atcaatttat aagaaaagaa
     1201 ggaagataaa aatgagcaaa atcaatttag agaacctaac aacggaacgc agaaaccaag
     1261 aaacttttgg attagatgag atgagtattt ctcaagccgt tcaactaatg aacaaagaag
     1321 atcagaaagt ggccgctgcg gttgaaaaag aactgacaag tatcgaacca gtgattgcta
     1381 acacgattga gtcctttaaa aaaggtggac gtctgattta tatgggagcc ggaacaagtg
     1441 gaagactagg tgtgttagat gctgctgaat gcgtaccgac attcggtgta gaaccggaaa
     1501 tggtcatagg tctaattgct ggtggagaag aagcaatgac tgtagctgtc gaaggggccg
     1561 aagattctta tgagctaggg caagaagatc taatcaagct gaatttgaca aaaaatgata
     1621 tagttattgg tatcgcagct agcggtcgta caccatatgt gatcggtgga ttagattacg
     1681 caaaatcgat cggtgctgtc acaggaacga tttcttgcaa taaacaagcc gaaatcagta
     1741 aacacgcgga tttcccaatc gaagtcgatg caggtccaga atttttgaca ggctctactc
     1801 gattgaagtc aggaacagca caaaaactta tcttgaatat gatttctact atcagtatga
     1861 ttggtatagg taaagtattc aataacttga tggtcgatgt caaaccaaca aatgaaaaac
     1921 taatcgaacg ctcaaaacga atcatcatgc aagcgacaga agctgattac gaaacagctg
     1981 aaaagtactt tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg attttgacaa
     2041 atagtgataa agaggaagct caagcaaaat tagctgaagc aaacggattt gtaaaaggga
     2101 ccttatgagg gggaaaagac catggcaaaa aacgaactga ctttggaaga actggcaaaa
     2161 caaatttata aaggggcagg cggcatgggc aatgtagaaa gcattgtcca ctgcatgact
     2221 cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg tctctgaatt aaaagcgatt
     2281 ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg acctgggaaa
     2341 gtgaacaaag tagcacagca aatggttgat ttggcaggcg tgaaattagg agaaaaactt
     2401 tcttctgtaa gtacagctac agcaaattca tcagcatctg gaaaagaccg tgtaaacgaa
     2461 cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa aatcaaatcg
     2521 gtattaaaag acatttctaa tatctttgtg ccaatgattc ctgcatttgt cggtactggg
     2581 atcgtggcag ggattgccgc agttttagca aacttagtca cagccggaac acttgatgca
     2641 gcaacatggc aacaatatat cgatgtgatg aacatcttga aaaacgggat gtttagctac
     2701 ctagttatct atacaggtat caatgctgca caagtttttg gtgcaacacc aactcttggt
     2761 ggagttatcg gggccgttgt gatgctgaca gggatgaatc cggaagcacc actgaaaaac
     2821 ttatttactg gtgaagctct tgcagctgga caaggtggga tcctaggtgt catcttcgct
     2881 gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga tgcggtagat
     2941 atcatcgtta caccaactct ttcattgatt gccatcggtc tgattgaaat tttcttcatc
     3001 atgcctttag caggtttcat ttctgatggc atggtcggtg ggatcaactg ggtacttggc
     3061 gttggtggag catttgccgg cttcgttcta ggcacattat tcttgccgat ggttatgttc
     3121 ggactgcatc aaattttgac tccaatccat gtgcaaatga ttgatgaaac tggtcgtacg
     3181 ctattgcttc caattcttgc gatggctggt gcaggacaag tgggtgcagc attggcatta
     3241 tggattcgct gcaaaaaaga taaagaatta acagaaatga tcaaaggagc tttaccagtc
     3301 gggatcctag gaatcggcga accattgatt tacggagtaa cattacctct aggacgccca
     3361 tttattactg cttgtatcgg tggaggtatc ggcggggcag tgatcggaat gatcggaaat
     3421 gtcggggcta tcgctattgg accttcagga gccgctttga tcccattgat ctctgacggt
     3481 aaatggttag gctacatcct tggattattg gcagcttatg ctggtggctt cgttgctaca
     3541 ttcttcttcg gtattcctaa agaccgttta gaaaaagaag aattagcgac tgaaacagcc
     3601 acagtaaatg aagtgcctga aaaagtagta cctcaaactt cagctgaaag tgtgactatc
     3661 ctctcatcag ttgctgacgg aacagttgtt tctttagata aagcaagtga tccagttttt
     3721 gcacaaaaga tgatgggtga aggctacttt gtagaaccag aaaacggaca tatctattct
     3781 ccagttactg gggtagtcag ctctgtcttc ccaacgaaac acgctattgg gatcacaacg
     3841 ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca cagttgactt aggcggtacg
     3901 ccatttgatg tgaaagtaac agaagggcag caagtgacac ctgatacgct tgtagcagaa
     3961 gctgacttag aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt cttggtaacc
     4021 aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa agcaaaagca
     4081 cctgtgatgg atgtcaaagc ttaatatgat agaaacaata gaaacgaaag aagaaactga
     4141 aatctatttt tcagcttctt cttttttatt atttattgtt ttctgctaaa atctgataaa
     4201 ttcgtgtcac atcagatggt gttccccgta attcataatc tgccaagaaa ggaaagccaa
     4261 atgtttgtgc atattgtttg gctgttagac aatactgatt attgaagttt ttatttccgc
     4321 taccgactac accaagacaa agtttatgat tgtcttcatg ttccagatat tcacgcatcg
     4381 tttcggtcaa gatttctgta tcaccgttat ctaaaccatt tccgccgtct aaataagtag
     4441 gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc actattttca tggatttctt
     4501 ttaaagtaac ttcaggattg acaggatctt ctgcatgcat tttttcagca tattctgcta
     4561 gatgtttggc aaaggcacgc gtatttcctg aaatcgatat atataaaatg ttcatggatc
     4621 atcctccaat tctttccttt tagtatagca tcttttttaa gttttttggt ggtgtaattt
     4681 ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa ttatatttct
     4741 attaaagttt ttattaatta agtaaaaata aaaaaggttt gttatacttt ttgataaaag
     4801 cggttttatt ttggaggatg cttacatgga tacagagcaa cagaaaaaag gaattggttt
     4861 aattccactt gctgccttag taattggttc agcgattgga ggtggcgtat ttggtattat
     4921 gacggacatc tccggatcgg ctggcggccc agctattttc agttgggtac tcgtaggaac
     4981 agcaatgttg atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc cagatttaaa
     5041 aggcgggatc tttagttacg cagaagaagg ttttggccgt ttcgctggtt ttatcagcgg
     5101 ttggggttac tggctcacat gctggttagg caacgtagct tttgcaacat tgttgatgag
     5161 cgcattagga tatttcttcc cagtatttga aggtgggcaa aatctggcat cgatcgtgat
     5221 gagtacagtc tttttatggg gatatgcatg gctagtgaat cggggagtcg aaaatgctag
     5281 tttcgtcaat gctatcgtca cattctgtaa attagtgcca ttatttttat ttattgtagt
     5341 agctatcagc acgttttcga tccaacaatt cattgataac ttttctagcg acattatatt
     5401 gaatgcgaat ggacagccga tcccgttttc cagtcaagta ttgagttgca tcatggtgat
     5461 gctttgggta tttgttggta ttgaaggagc ctctgtcgta ggttcacgag caaaaaaaca
     5521 atcagatgtt gggaaagcaa cgattctagg gatattcggc ttgattatta tttacgtctt
     5581 ggtttccatg cttccatatg ggacaatgac gatgaatcag ctgcaaacga tcaaggcgca
     5641 gcctgctatg ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag tcgtgatcaa
     5701 cggcggtttg atcatttctt tagttggtgt ttggctctca tggacgatct taccgattga
     5761 aacaatggga aacatggctg aagacggact tttgcctaaa gcatggggaa aactcaacaa
     5821 aaatggggca ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg tctttttact
     5881 ttcattatta ttcactgaca gtgcttataa ctttgcttac acacttggta cagcagctat
     5941 tttcttcacg tggcttttct taggattgta ccaaatgaaa ctttcttatc agcggaaaga
     6001 atgggtccaa tttttagtag ggcttttagc aagtgccttt caagtatggg ctatgctagt
     6061 cgtagccttt aatgaagtaa tgcttgttct tgttcttttc ttgccaggca tttattttta
     6121 cctcaaagca cgtaaggagc aaaacgagcc tgtacaacat atccacttag ataagatttt
     6181 acttataggt gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca ctgggatgct
     6241 tcacgtatag aagtataaaa gaagtataaa gagaggttgt gagcctgtcg tttattcaaa
     6301 aacatactag gaaaggagct gtgacacaaa tgttgtcaca gctccttttt cagaataaac
     6361 ggtgtttaag agacagccaa tcggcaataa ggctgaacgt ctctttcaca gcctcttttt
     6421 tgatgagtca tcatacattt tttcgcttaa atacgagata tcctaatgct aagaacaaaa
     6481 cgatgtaagc taaattaccg aatagcattt gattagtaga aagatgagtc aattgcttca
     6541 tgatctcttc gtttccaatt tggttttgca gattcaacat actgatcgga ttccatttga
     6601 tccactccca tttttggatg agtgcaataa ggataccaga tatgattgac gaagcaaaat
     6661 aaaagacgat tcctgctgag atggatgcgc cggaactatt gataaagcaa gcaatcatca
     6721 acactaaact taaaatcagc cataaaccga tataactacc caaagtataa gtgaacaagc
     6781 tttggatcag tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa gggaaaagaa
     6841 tcagtttcat caacacagtg acaataatag ctaaaaggta taaataaaca gaataaataa
     6901 ctaatgtgat ccatttgctt actaatattt ctcctcgaga atactttcga taaaggagat
     6961 ttttcaatgt accatattgt gcttccatcg aaatgatcgt gcttgcagct gcaatcatga
     7021 taaagacaat ccaagatgtg gcactaaaaa gctgagcagt catttcaact ggatcaatga
     7081 tcgtcgtgta ttttttggct agaagtgctg ttcctatgag caaaacaact aatagaacag
     7141 ataaaacagc agttgacttt ttcttgatca atttaaataa ttcttgtttt acaagcaatg
     7201 tcatttttct tcttccccct ttactgaatc cagaatttcc aatagagaag attccaaatc
     7261 attcgtcaga tgctggacgt ctgtcacatg gatattttgt gcagacaata gctggatcac
     7321 ggttcctaat aattcttcgt tttcttttaa aagcttgatt ttatcaggat cttccaaagt
     7381 atatccagct tctgtcaaga tcgttcgagc agctagatca tttgttgtag agataacgat
     7441 gaaatcttgc gctgtttggt tcaattcagc cattgtacat tgtttgatga tctttccttg
     7501 attgatcagg atcagctcat cgaccatttt ttcaagctcg ctgagtaagt gactggagat
     7561 caagaaagag gtgccctgag tagccaagtc ggtaatgatt tttcgcacat ctcttgttgc
     7621 ttgtggatcc aaaccgttca tcggctcatc taaaataacg atagacggat ggttcagtaa
     7681 ggcaagagca attcccaatt tttgtttcat tcccaacgag taggatttcg cttttttgtg
     7741 aatgtacttt tccatcttga gctgattcac gatagtgtcg atagcagata catcgttggt
     7801 ttcagagaat aacttcagat gatcatagcc tgttaagaat ggatagatac ccgggtattc
     7861 gatcaatgct cctacttgtt ccaacccttt atgactagag ggagaaatct cattttcacc
     7921 aatacagatt gttccatctg agtaatggat cagtcctaaa atagctttca tgatcgttgt
     7981 ttttccagcg ccattaggac ctactaatcc aacgattttg ccaggggata agctaaaact
     8041 gacatcgctg agggcttgtt tgtgaccaaa gttcttggat aattcttgga ccgaaagact
     8101 tttcaaaaat attcactcct tttctataga tagtacaagc atacacctga tgagtggaga
     8161 agtcgctagg tcattggact tatttcttat tttttcataa aaaaattgaa tattaaataa
     8221 aaaggcttat tttttctgat cagtacttat tccaagtgtc tagtggccgc tagtaatctt
     8281 gatgccaatg atactgatga gtagcatccc cacaaagaac caagtggaaa gattcagctt
     8341 atcatgaaac aagaccacac caattatgac cgaaccaact gcgccaatcc ctgtccagat
     8401 aggataagag agactcatcg gcaaactttt gattgccttt gacaaaaagt aaaaactagc
     8461 aatcatgcca atcactgtgt aaaaagaata attgatttta gaaaaaccat cactccattt
     8521 catcatcgtt gaccagaata cttccaaaat tcctgctatg attaattcca tccatgccat
     8581 acttacattc ctccttaact ataaaaaaat acacaaaaag aggaaaatca tcccttcaaa
     8641 gacctaatgg aatgatcatc ctctttatcc tacccggtgg cagattgtat atctttttct
     8701 gtagtatagc acacctatcc agatatcggc aatctcatac tttttagtaa agtgtaccaa
     8761 gcactgacgg atatatcgtt agcggaaaag aagatgaatg ctttgcaatt tcatagaaag
     8821 aagttgttat ttattaatca tttaaaaaaa
//
LOCUS       DAHUWM010000111         8773 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHUWM010000111 DAHUWM010000000
VERSION     DAHUWM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.112"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..292
                     /locus_tag="NK788_001847"
     CDS             <1..292
                     /locus_tag="NK788_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770175.1"
                     /translation="TVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRT
                     RSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(389..823)
                     /locus_tag="NK788_001848"
     CDS             complement(389..823)
                     /locus_tag="NK788_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770176.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1055..1765)
                     /locus_tag="NK788_001849"
     CDS             complement(1055..1765)
                     /locus_tag="NK788_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCH6770177.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1755..2615)
                     /locus_tag="NK788_001850"
     CDS             complement(1755..2615)
                     /locus_tag="NK788_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCH6770178.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2620..3258)
                     /locus_tag="NK788_001851"
     CDS             complement(2620..3258)
                     /locus_tag="NK788_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCH6770179.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3273..3572)
                     /locus_tag="NK788_001852"
     CDS             complement(3273..3572)
                     /locus_tag="NK788_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770180.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3615..5099)
                     /locus_tag="NK788_001853"
     CDS             complement(3615..5099)
                     /locus_tag="NK788_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCH6770181.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5119..5580)
                     /locus_tag="NK788_001854"
     CDS             complement(5119..5580)
                     /locus_tag="NK788_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6770182.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5598..6665)
                     /gene="ulaG"
                     /locus_tag="NK788_001855"
     CDS             complement(5598..6665)
                     /gene="ulaG"
                     /locus_tag="NK788_001855"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCH6770183.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6883..7653
                     /locus_tag="NK788_001856"
     CDS             6883..7653
                     /locus_tag="NK788_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6770184.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7926..8753)
                     /locus_tag="NK788_001857"
     CDS             complement(7926..8753)
                     /locus_tag="NK788_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770185.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2651 a         1852 c         1380 g         2890 t
ORIGIN      
        1 tactgttgaa tttgacagtg aactacttaa gcaatttcca caagaagaac atttcatgta
       61 taaagaagca atttcccaca aagtgacaga gtatttggag cataattttt ttcgtataaa
      121 gccagtgcgt actcgttcgg caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa
      181 aaattatcgc atagcatttg ctttaaaaaa aaatcatgtg ttcatttttt atattagttc
      241 gaatttgcaa aaaattgtat ttgataaaga ggtgagtaaa aaaataaaat agctgagcat
      301 tggaggatag attgagtgat actaagtcta tcctcttgtt tttctaaaga actaagctag
      361 aattttttct tttgtacgtt attatatgtt atttgtcatt accaaaaagc accttttttc
      421 ttctagtatc tatgacataa gaaataccac ccatttcctt tacttgaaca aatcctactg
      481 attcataaaa tttttttgtt ttctcagtat catctgtcaa taattcgatt tggactggtc
      541 cataactagt gatcttctcc aaagcttcat tcaaaagcac tttgcctatt ccttggcgct
      601 gatacctcgg atgaatgagg atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc
      661 aacgaacgat tccgataatc ttcccttccc cattacgctt tatcagagat tcagattgtt
      721 caaatgctct agctaacctt gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat
      781 ataattcaga aatctcatcg atcataaggg aatcaatatt catttttttc ttcctttctt
      841 atacaattct ccctctaata atacagttct ttgactattt aatctatcac atataactct
      901 cctgtaaaga ttctctcgat tccttaaaat tcctctttcc ttttagaatg catacttttt
      961 gataaataaa aaggttgtga taaattttct tcacaacctc tgtttctctc tatcaagtgt
     1021 tatcttttaa ttttctaccc ttaccctagc atctctattg cccgtagtat gcatactttc
     1081 catgtttgcg aaaataatgt ttatctagta aatattgcgg gatttcacat atattggggt
     1141 tgatcagctc gttttctttg gccatcagac aaatttcatc taagatcaaa ctgttttcta
     1201 ctgctttcat aggtgaatcc ccccatgtga acggtccatg tccatagaca agtactccag
     1261 ggacttcgtt tggatctaat tttctttcct taaacgtctc gacaatcaca ttccctgtat
     1321 gaacttcgta agcttctctt acttcttctt tcgttaattg gcgtgtacaa ggaaccttac
     1381 cataaaaagc atcagcatga gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc
     1441 acatgacgga gtgcgtggaa tgagtatgag taatagcagt tatatcttca aaagtttgat
     1501 acaaaacaac atgtgtgggg agatctgaag atggttttaa gctatcttct tctattatat
     1561 ttcccgacaa atctgttacc accatttgat ctgcttgcat gcattcatat ctgacaccgc
     1621 ttggcttaat gacgatgact cctagctcac ggtccacttc actcacattg ccccaagtca
     1681 atttgactaa tccgtatcgc ggcaatgcca gatttgcttc aaggacacgt tgcttcattg
     1741 ctgcaatttt ttcttcacaa gacatcatag ccagcctcct tcaatttaga acttaagtag
     1801 tcttttgcct gctggatttc tcttcgaaag tcttggcttt tttcactcca catttcaatc
     1861 agaaatgttc catcataatc tagacgtttc aaatgtctta ataatccaag aaaatcaaca
     1921 caaccgctac caaaaggaac atcacgaaac tggcctgaat attcttttgt tacagggtaa
     1981 gtatctttta agtggatagc agtgatataa tcgatccctt tctcaagttc tcgagccgga
     2041 ttattttccg gccatgcgga caaattgcct aggtctggat atacttgtag aaaaggtgaa
     2101 tgaatctgtt ctttgatttc taaaaatttt gaaatggagt tcataaatgg atcatccatg
     2161 atttccacgg aaagaatgat gccatattta gaagcttcct ttacgctttc ttttattcct
     2221 tcgataaagt cttctctgga attcattgat ttctcttcat aataaacatc gtatccagct
     2281 atttggatta ctttgacgct tagattatgc gccaaatgga ttgctttttt cattagttgt
     2341 ttcgctgctt ttttcttttc tggatcagca gaaccaaacg gaaatcgccg atgcgcactt
     2401 aaacaaatcg aatggatacg tacaccggag ctgaacatct cttctctcag ctcagctatc
     2461 ttttcttcag accaatctaa tcgtgctaac cgctcatccg tctcatcaat cgacatctcg
     2521 ataaaatcga agtccatttc ttttacaagg gaaaaacgtt ctttccatga aatatctttg
     2581 ggtaacgctt tttcataaat cccaatttga gccatttcct tacccccaga tacgaatgat
     2641 ctcatctttg aagttttgtg cggccttcat cggatcattt gctgctgtaa taccacgacc
     2701 agtgataaag gtatacacat cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc
     2761 accagttaca gaaacattaa agcctagttc aatcagtttt ttgatcttcg ataaatcttt
     2821 ttcaccccaa gtttcaccag ataacagcgc atcacggctt tgatggtaga ttacctgccg
     2881 gataccgatg ttatgccaat ccatggcttg ttcaaaagtc caattgccgt agagttctac
     2941 ttgcaattca cctatttctt tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat
     3001 gactgtcatc cagtctgctc ctgcttttgc aacattactc gccactgttc ctccagcgtc
     3061 tgcacatttc gtatcagcga ctagtttttt atctgggaac atactgcgaa tacagcgaat
     3121 cgctttttgt ccttcctgta agcaaagaat tgtgcccact tcaataatat ctactacatc
     3181 accaactttc ataacatctc ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg
     3241 caaattcggc ctgctcatct tagttccttc ctttacttgt ttttttggta ccactttgtt
     3301 tcagcaaatt tttcactgat ttctttgact gacatcacat ttttgatacc gataactgta
     3361 actccttttt tctctgcctg agtaaacata ttcaaaaaat tgactggcgt aaaaactaca
     3421 tcgtattggc cagctgaact ttttccctct gaaatggcac aatgatggat tttggtaatt
     3481 ggtacgttta gttctttcat tgctttttcc acacttctca tcatcatcaa gcttgttcct
     3541 gatccatttg cacacgatac taaaattctc atcttaatcc ctccattatg agtgttgtct
     3601 aatcagattt tcttttatac tctctgcttt tctgcatatg tgtcatagtc ttctacaatc
     3661 aaaaaataac catcaggatt tttccgatat tgtaactgcg gaatagccaa taaaccaatg
     3721 acaactacgg caattccagc ataacctagg actttcatga ttactgtaaa caatggccaa
     3781 gctgttgccc agtcgaacat cccgatataa ccaccgtatg ttgacatccc aatccatgta
     3841 gcgaataatg ctgaaccgcc tacttgtatg attcctgaaa gaaacgggaa caggcaagcg
     3901 gctttgaatc cacctcggtt attggcataa actgctataa cggcattatc aaagaacaac
     3961 ggaatgaaac cggctatgac gatcgttggt gatttcaata aaatcagtaa accaatcatc
     4021 aaaaactgtc ccaaagcacc aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag
     4081 ccaaaagtag cagctacgtc aattcctgga acagctcctg gtaaaatcgt gtttgagatt
     4141 ccttggaaag attgagttag ttcatcgacg aatgtccgaa cacctaattg caagatcgcc
     4201 agatataccg caaaatttaa agaagtcgtc ataatgtaga ataagaaact ctgcccttct
     4261 tgcatgaatt gcgcttgaat caaataatct ttgcctaata caatcagaat gatcccaaag
     4321 aaaaatagca tgagtaacga agtagcaacc atattctcat tgaaaatcga caagaatcct
     4381 ggtaatttga tatcttcaat ttttttgtct tgcttgattg atctattttt cttttcatcc
     4441 cgctttttca tccactcagc taattttgca aatataaaga ttccgaacat ttgctgatgt
     4501 gccactgcaa atccggcacc ttctgtcaat tcctgcgtga ttcctactgt taagttagaa
     4561 gctactgtcc aataacaacc taaaatcagt cccataaaaa tcaatgcttg tatcctacct
     4621 agatcaggaa agcaaaacag taaaatccaa aaagcggtag ctgcttgttg aacctgaacg
     4681 tttccggtag tgaaaacgga acgcagtttc gtatatttct gaaaacggac taataagata
     4741 tttaaaacaa atgcaatcag taataaaatc atcacatctc caaaggtacg gccaaatgtc
     4801 tccattaatc cagcatccac agcattttgt ccaaaataag gatctgtaac catggcatcc
     4861 aaattgaacc gttcctttag tccaaccaag attggccgaa aattattgac caaaccacca
     4921 gagccaacag tcaaaataaa gtaaccgacc gttgccttta agaatccagc gatgcattca
     4981 tgaagcgggc gtttcagcaa aatatagcct aataacacga taaaaccaat caggtatgct
     5041 ggctgcgtca agatatttgt tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc
     5101 ttttttctcc tctatcaatt aattatattc tgccatcaca acttggtaat catcgatgga
     5161 gttcgtagag atgagacgct caaccaatcc atctgtcatt aataattcgg ataatttttg
     5221 gatattttct agatgttctt ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt
     5281 tttctcctca ttctcccctt cactttcaaa acggattggc tgtttcaact tggtaaatga
     5341 aatagctgtt ccaaaaacac catcactttt ttctgaagaa tgcggcattg ctacttgagg
     5401 tgcgatcaca atataaggac cgtatttttc tacacaatgg atgatctcct ccacatacgt
     5461 ttcatcaata atttccttat cgatcaatac aagacagctt tcggcgatag cttcacgcca
     5521 atccgtgatt tctttatctg ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa
     5581 gaaactcccc ttctttctta taaaaacgac ggaaacggtg tatcattttc aatcgagaag
     5641 acatcttcaa atcctcgata aaaattaaac tcaagatcat ctttgtttgt cggatagatg
     5701 aatttcccgc ctacttgcca gatataaggt ttaaatccat acttcaggcg atcctttttg
     5761 aatttccaaa tttcagtgat ctcttttgga tcagccataa aattagacca aatatcgtag
     5821 tggacaggaa tcactacttt tgcattcaat gactccgcca tgcgcagcat atcaacagaa
     5881 gtaactttgt ctgttatccc tcgtgggttt tcaccatagg cccctaaaca gacatcgatt
     5941 ttgtgttcat taccatgttt ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa
     6001 agattccctc ctgatgtttc gaataaatag tttactgcaa tttgatccat ctcctgaggc
     6061 atttttcctt ttaatacctc ttctggatct tcacaagtga ccaacgctgt ccgatcaaac
     6121 gcttctaaag ctactacatt gatgtctttg attttgactg catcacctgg ctttactgta
     6181 atcgtttttt cttcaggaat tccccatttt tgccacgtgc gaaccacttc ttttggccca
     6241 atgaatgttg cttcaggaca attttgatga actgctgcgg ctgtatggat atctagatga
     6301 tcggaatgga tatgtgtaac gattaaagcg tctacatttt ttatcgcaaa aggatcgatt
     6361 acgaaaggct gagtacgtaa attaggctgc atattttgaa cgccactcat acgcatcatc
     6421 tgatggcctt ttttcatcat gccattccca tgactacgtt ttcccgtccc acaccataga
     6481 tcacataaaa tattcgttcc ttcatgactt ttcagccaaa ttcccgtaca gcccagccac
     6541 cacatagcaa aagttccagg atccactact tctgtttcga tttcttcatt caagtatgtg
     6601 ccccactcag gaaatgtttc tactaaccac ttttcttttg ttacttcatt gacagtttta
     6661 ctcatttgct tgctcccccc atatcgttgt tccaacttac aagtaaatat taatgtgtat
     6721 cagcatccat gaaaaccctt ttatatgacc gtttgttttt tatatgattt tctttcagta
     6781 atcaatgatt gtttcagtat aataaagaca gatatgaatg tttaaatgaa caactagtca
     6841 aataaatcca tggatcaaga tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc
     6901 aatattgaaa aacgtcaaaa agaaattttg gaactgcttc agcagactga acaactatca
     6961 actttcgaat tagctgaatc ccttgatgta tctattagta cgatacgaag agatttaaat
     7021 ttattagaag aaaaaaacga gatcatacgc aattacggtt attgttctta taattacaaa
     7081 aaccagactg attttgatca atctggtcct gaaaggatta aacaagcgat tgccagagaa
     7141 gctagtaaat acattaacga ttatgatacg ctgtttatca attctagctc gactgcgttg
     7201 aaaacactgg attttcttca atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc
     7261 aattactctc cgcacaaaga taattgtagt tatattttaa ctggtggaga gatgcgcttt
     7321 ccaaaagaag cactagtggg cgacatcgca ttaaatacga tttcttcagt caatgcagac
     7381 atttgtatca ttggctgtag cggagtcgat gtagtacatg gggtcacaac aaagatatta
     7441 aatgaatcaa cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa gattttagtc
     7501 gctgatcatc gaaaaatcgg actgacatca aagttcaaga ttgctgatat ttcagtcttt
     7561 gattatttga tcactgataa attttgttct gaaaaagtat tagaggagat cagaaatacg
     7621 gggataaaaa ccattcaagt agattcattt taagaaccag gacgtaaata tgtaaatagg
     7681 attatcaaaa aaatgaataa caaatttatc gataaatgaa aaacacgcta cgctcaaatt
     7741 gataaaggaa tcccaagatg tatccatcca atcgatacca tcataatcta ttgcttcttt
     7801 tataaccgtt atatgttgcc taaataggaa tcaaacaata tcgaaaaaag gaggtatgac
     7861 aaaagtcaca cctccttttt tcgaataaac ggtattcaaa tcagaacact gatttcacaa
     7921 ccactttatt tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc
     7981 gctatttttt ttgcgtccat atatctttct aaataatcat cttctcctaa ttgcttatgg
     8041 caagaagcaa tttcataaag caagtattcc aatgcataag tcgatttatg tttcttatta
     8101 tactcaatac ccttctcaca taattggatt gctttatcat aatcctcgat tttacggtaa
     8161 taggtggcga tatcataata tacaatgcag accatcagat tatcaaaatc agtctcactc
     8221 aatattttaa gtgtacgctc cacataaact ttcgcaaaat ctaattctcc ttttgacaag
     8281 tagatcattg ccacgtttgc agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt
     8341 tccaacactt gattaaaata aaataatgct gcatctggtt ttttcgcata aagatgatca
     8401 agcagtcctt gataaaataa aatttgattt aaaagatcag aattacttac tttttcctga
     8461 tcgatcgttt gtaaaatttt ataagattct tggtattttg atacagaatt caatgattga
     8521 atctcattta tttttttcct gattttgtct tcttctgttt ctactaatat atcggagatc
     8581 tcgacattca gctttttgca aagcgcactt aagatattga tgcctggcga catattcttg
     8641 ttttcgatat tgctgacagt tgcttgttca caaatgcctt ctgctaattg tacttgtgtt
     8701 aacccctttt tctttcggta ttcttttagt aatgctccat ctactttaat caaaacaatt
     8761 tccttccctt taa
//
LOCUS       DAHUWM010000112         8747 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHUWM010000112 DAHUWM010000000
VERSION     DAHUWM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.113"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            101..2542
                     /locus_tag="NK788_001858"
     CDS             101..2542
                     /locus_tag="NK788_001858"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCH6770186.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="NK788_001859"
     CDS             complement(2872..3576)
                     /locus_tag="NK788_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770187.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="NK788_001860"
     CDS             complement(3763..4008)
                     /locus_tag="NK788_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770188.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="NK788_001861"
     CDS             complement(4025..4876)
                     /locus_tag="NK788_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCH6770189.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="NK788_001862"
     CDS             5042..6046
                     /locus_tag="NK788_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCH6770190.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6829..7386)
                     /locus_tag="NK788_001863"
     CDS             complement(6829..7386)
                     /locus_tag="NK788_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770191.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(7565..8176)
                     /locus_tag="NK788_001864"
     CDS             complement(7565..8176)
                     /locus_tag="NK788_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770192.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(8289..8546)
                     /locus_tag="NK788_001865"
     CDS             complement(8289..8546)
                     /locus_tag="NK788_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770193.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT         2738 a         1626 c         1643 g         2740 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctccttat tccgaataaa tggtgtccaa aagcttgcat ctgccagatt
     6301 atctcatttc ttatgacaag ctaagcaaat tatctccacc aaataattta ttcatatttt
     6361 tgacttgcgc atactaacta attcttaaat tttcatacaa aatttctcaa tttcccttaa
     6421 cgagtaatat ttatgtacgg tgattctcac tagtatccca atcacttttt aagttatccc
     6481 gatttcaatt ctacttcaaa aatagttgct tttatgtatg ctgttagttg attagatggt
     6541 catttgttga gctaggtcgt ttattttaaa caagttgttt ctttagctta tacctatctc
     6601 gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca tctgtatttg ccactttgat
     6661 tcgtataaag ctaacaacta ttaactcctc tcagctaata gttgctctct tttttattca
     6721 gattcatttt tgctgatcac agcctagtat ttcaatggtt ttgaacacta aaccaaattg
     6781 ttccattaca acattcactg gctgatgaaa tggattagta gatttcgatt attcaaagcg
     6841 attttctatt ggtggatcgt gttcccaact aataagacgg attccaaacc tatcgtacag
     6901 atcttctaaa ccacttataa attttgcacc tatttcaggt gttttattgc gtggatattc
     6961 tattgttacg ccattaaatg gataccaatt aggattaggc tctctttttt tctgtacatc
     7021 tcccatagaa tagttagcac tttctgggac ataatatatt cttcgaccat cctctaattc
     7081 atctaataag atatttcgtg atcgtctatc acttgctaaa tgtagacgcc acgtgcgata
     7141 ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc
     7201 tatcacaatc ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt
     7261 aatttctggt cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc
     7321 cagatgaaac acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt
     7381 tctcattatt cgcacatcac ctctacattc tcacatatta gttcgtaata ctcctttaat
     7441 atcaaaatat ctactggtcg taataatatt cgctatgaat tcctactact cgtataagaa
     7501 aggccaaatc atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg
     7561 ttttctaatc accaaaattg ttaacaatcg gttcagatat ttcccagttg attaaggtaa
     7621 ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt aagtacagtt cttatgtatc
     7681 cactttctcc taatataaag tctacctctc ttttaaaacc ttcctcgaga tttctaaggg
     7741 ctatatgtgg ttttaatgac attgattgta cattttcccc catataatat tgcgcacttc
     7801 ctcgactaaa atagattcct agatcgtccc caaaaggtag atcttctcta ccactagcta
     7861 atctacttct ccatcttcgt tctcttttca tagaacctgc gctgaactca cacccgttaa
     7921 actcagctat gacagtatct tcaattatgt taatctgtcc atctagttcg tattcaatcc
     7981 taaatggaaa ttctccatag gtgatttccg gttcgggtat tttaggtgag attaatgcaa
     8041 agaaaggaaa tattaagctt ggcaacatca aaaaaactcc tcctatgatc acaattcccg
     8101 atgtaatcca aaatataatt tgtacaattc tcattgatcc attgtattcc attttattcc
     8161 ccctattact attcatatcc gcctatctat atcgatcttc caatgcagag ataattgctt
     8221 ccataccttg attttttact gagttaccta tgcgagatat cattctccac taaggtgcat
     8281 catattgtct atctaacatg attaattctc caataggtgt atactcctcc aaaaacaaca
     8341 tttaatgcat ctccctcgac aacataagct accatgtttg ctgtatatgt gtttgagtct
     8401 aattcataag cagataaatt tactgtcgct ggattaaata taatcgcatt cgtatttata
     8461 gctactgaat ttgctgcttc agcaccacct tttgaatgtc cgatcattgt gatttcaata
     8521 gtaggattta cgtggacagc cctcaccgtt taccctgacc attatagaaa tcgcttactt
     8581 ttttgaacta cgcaacaaag gatacaatgc tcctaacttc actgtctcca tttttgtcca
     8641 ctaaggaata taaaaaatag gcattaattc atttaattcg gtcacaacac gttacctttt
     8701 aaatcattat actgtatctc atacattatt atcaataaac tattata
//
LOCUS       DAHUWM010000113         8675 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHUWM010000113 DAHUWM010000000
VERSION     DAHUWM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.114"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1101
                     /locus_tag="NK788_001866"
     CDS             <1..1101
                     /locus_tag="NK788_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6770194.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="NK788_001867"
     CDS             complement(1280..1609)
                     /locus_tag="NK788_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCH6770195.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="NK788_001868"
     CDS             complement(1599..1871)
                     /locus_tag="NK788_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCH6770196.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="NK788_001869"
     CDS             complement(2322..3125)
                     /locus_tag="NK788_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCH6770197.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="NK788_001870"
     CDS             complement(3103..4227)
                     /locus_tag="NK788_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770198.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="NK788_001871"
     CDS             complement(4243..5058)
                     /locus_tag="NK788_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6770199.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="NK788_001872"
     CDS             complement(5048..5920)
                     /locus_tag="NK788_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6770200.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="NK788_001873"
     CDS             complement(6005..7294)
                     /locus_tag="NK788_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6770201.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="NK788_001874"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="NK788_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAHUWM010000114         8645 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHUWM010000114 DAHUWM010000000
VERSION     DAHUWM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.115"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(124..207)
                     /locus_tag="NK788_001875"
     tRNA            complement(124..207)
                     /locus_tag="NK788_001875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Leu,seq:cag)
     gene            735..1235
                     /locus_tag="NK788_001876"
     CDS             735..1235
                     /locus_tag="NK788_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770202.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1425..1769)
                     /locus_tag="NK788_001877"
     CDS             complement(1425..1769)
                     /locus_tag="NK788_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCH6770203.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1923..2843)
                     /locus_tag="NK788_001878"
     CDS             complement(1923..2843)
                     /locus_tag="NK788_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6770204.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3030..3521
                     /locus_tag="NK788_001879"
     CDS             3030..3521
                     /locus_tag="NK788_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCH6770205.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3545..5920)
                     /locus_tag="NK788_001880"
     CDS             complement(3545..5920)
                     /locus_tag="NK788_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCH6770206.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6246..6872
                     /locus_tag="NK788_001881"
     CDS             6246..6872
                     /locus_tag="NK788_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCH6770207.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6895..7599
                     /locus_tag="NK788_001882"
     CDS             6895..7599
                     /locus_tag="NK788_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6770208.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7791..8624
                     /locus_tag="NK788_001883"
     CDS             7791..8624
                     /locus_tag="NK788_001883"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCH6770209.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2622 a         1647 c         1536 g         2840 t
ORIGIN      
        1 ttttttttga tttatttgaa tctggttccg ttgtttaaaa tttttcctat attcttttag
       61 ataatatgtt attttctttc tcatcgctcc aggtgtacct tctctttata aaaaaacaaa
      121 ttatgcggct aagaagactc gaacttccac gaggtcaccc tcactagcgc ctgaagctag
      181 cgcgtctgcc aattccgcca tagccgcatc acatctttta gtttaggatt acatgtgttt
      241 tttgtcaaga acatattttt gttctttttc atttctgtac gccctccctt atgccatcta
      301 cacaaataat tcatccgagc aactccatca tctattgagg gttggtgaaa aaatctgttt
      361 tatccatatg atcgatttca atgttaaacg aacgactctg ggattagtaa ctattttatt
      421 tttttcttca tttctttttt cctctttttc tcgattcagc tatcttcttt catttggata
      481 ttgattattt cttctgttga ttactatcct tctggaattc tagacgctga agtaattcac
      541 gttttctttc ttctagagat cataagtaac gaatgaacga acttcaaata atttttttat
      601 tgtaattcat atttgtattg ttcgatgatt cttaatacga tatgacacaa taaataaaaa
      661 taggcatggc tatttacaac aatagtgcta tagtagaaaa caaatgaata aggttactta
      721 aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc agatttaaag aaagcttatc
      781 agctacaaac tagctttgga ccaaatgaga gtggatttat caacaatgct tatggctata
      841 catttgctga atttttagat tatgtaaaaa aatgcgaaaa aacgtcttta ggaatcgatc
      901 ttcctgagag ttacgttccc gcaaccgtct ttattttaga aagtgatgaa catgagtatg
      961 taggaatttt taatttgcgc cataaactga acgaaacatt aagaaacggc gctggccata
     1021 ttggttacgg catctcccct aattatcgta aaaaaggata tgccacacaa ggattagcat
     1081 tagtactaca agaggcaaaa aaaatcggca ttgatgaggc acttttgtct gtaaataaaa
     1141 aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc tgcgatttat cgtgaagatg
     1201 agaaagaata ctatacgaaa atcaaactga tgtaaaaaag aaggattcca aaaggaaagg
     1261 gatttgaatg tcctttatca atagaacaca tttatttaat taacttataa tatagcagac
     1321 aaaattttat aaacaaagaa accttttatc tcttttcttc tagaaaggat aaaaggcttt
     1381 tttgtttttc ctatttgctt ctcatagcta gtcagccaag atttctatcg tatccccaac
     1441 gcaatccgtg cataacggct catcttatct gtcgtccatg ctggatacca gactagtttg
     1501 acttctgctt tcgttacttc tggcacttct tttaatgcgt catggataga ttctgttaaa
     1561 atatctgcta atggacagcc catagttgtt agagtcatct taatgactgt ttctccattt
     1621 tcaggattga attctatatc ataaatcaag cctaaattga cgatgtcgat tcctaattca
     1681 ggatcaatca ccatttccaa ggcagcaaga atacgttctt tgattagttc tatttcttgt
     1741 ccagaccgtt cttggtttgc ttcgctcatc gtacgacact ccttaacacg atttttattt
     1801 atcatactct ttttttagct gcttgtaaat gagaatttag aagtaagatt ctcgtttaaa
     1861 tttataaaaa tgttcttatt agatgtaata gattaaattt attctaaaat aaacctgatt
     1921 cctcattttt tttgtgaacg attttttatt tctggcaata cgccagttgt tcgatacatt
     1981 ttttgttcgt tttcatttgc aaaccggcca aatactgaat gagttggtaa aatattgata
     2041 aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat catacatttc gtagcgggtg
     2101 atgaccatca tgatcatgtt cccttgtttt tttgaatagc caccgacaga cggcagtaat
     2161 gtcattccac gtaccatccg ttttgctagt tcttctgtta cctcttctgg cttgacggta
     2221 acgatcattg ctgttacttt ttgatgactc gtgtggatgg catcaattac ttgagtcatt
     2281 gcataaatgg aaataatcgt atacaaggca ctttcccagt taaagaagaa gcctgcgatg
     2341 actacaatga taccatttag aatcagcatg tagtttccga ctgtttttcc agttgtttgt
     2401 gaaagtacaa gagagatgat atccatccca ccagttgtaa agcccatttt taaagataat
     2461 cctacgccaa gacctaataa aacaccgcct actagggcat tcatcaaaat attatcagtg
     2521 atctgtccga ctggaagcag catcgtcatc accgatacac atacaacatt cacaaaacta
     2581 aggatcgttg ctcgttttcc tactttgaga aaccctaata caaacacagg gatattcaaa
     2641 agaaaaataa atgttcctgt gtctatcttg atatgaaacc attcatataa aagcgaggaa
     2701 atgatttgag cgatcccatt catccctgca gaaaagacat tggctggaat caaaaaataa
     2761 tttaaagcga ccgcagcggt caatccggtt atcaaaataa ccactatttt ctttaatctt
     2821 tcatttttat gatacgcagt cataatttta ctcacaagca cctctcccct actttggtat
     2881 tccaaatgta agaataacag tttatctatt aaaataaaat acttcgaact caaatgagaa
     2941 gaattttagc aaacaggtaa gaatctgccc taatcctatg ataagataga tgtatggtaa
     3001 atatttatat gtatatcagg agggttccta tggcaaaaga tgctagcttc gacattgtat
     3061 cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca aacaacaaaa gaattgtcca
     3121 atcgctttga cttcaaggat tctacagtag aaatcaaatt agagaacagc aatctagttg
     3181 tattgagcga tgatgatttc aaaatcgaac agatcaaaga tgtattattt ggtaagctga
     3241 tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga atcagaacac gctctaggtg
     3301 gcaaagctcg acaaactgcc gatttggtca acggaatcga tcgtgaaaac gcgaagaaaa
     3361 tcactacagc tatcaaaaat gaaaaactga aagtaaaagc acaaatccaa gaagatcaga
     3421 ttagagtgac aggtaagaac cgtgatgatc tacaagctgt gattgcttta ttacgtaaac
     3481 ttgacttacc aatcgagttg caattcacta actatcgtta atcaaaaaac gaggctgacg
     3541 tctgtcattc ctcgtttttt tgatttactg cttcttttga taaccaaaaa cgctgtattc
     3601 cttcttccaa ttccccaaca cttcgtactt ctcttgctgt ttgccaatgt tgtgggaata
     3661 aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa tacaacgatt ccttgatccg
     3721 ccatgccacg aatcactcga ccaccagctt gcaagacttt gttcattcca ggaagctgat
     3781 aagcgtaagc aaatccaagg tgttccttct catcataata tgatttgatc agttcctgct
     3841 catgattcat ctgcggcaaa cctacaccta cgatcatact gccgatcaat cggtcccctc
     3901 tcaaatcgat tccttctgaa aaaatcccgc caagtacgca aaatccaatc aatgtttcag
     3961 cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc ttcctcattc atgtccgttt
     4021 cttggattag cacattgctg tcgggatatt gctgtctgaa tgcttccacc gcaagatcta
     4081 aatattgata agaaggaaag aaaacgaaat aattcccgac ctttccatca gcaaaagcat
     4141 gaatcgtctc tactaatttt gccagactgt gttccctttt tcgataagtc gtctctaggt
     4201 aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg actcggcagt ttgtaagcca
     4261 gacttttttg tccccccagt gtttcctgat aataagagag tggtgaaaaa ctagcagaaa
     4321 aaagaatact gctttttcct ttatccaacg actgctcaag aaacaagctt ggatcaatac
     4381 aaaactcttt tactatcaga tcgcgatacg ttttttcgac tgttgtttcg tagtgatcat
     4441 cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa ataataagga agcaattgtt
     4501 cttgtgatgg gtgctcagga aattctgcca gccattcttt aattttttcg cttaattgat
     4561 aacctgcctt tactaaagat tcagccggtg ctttttgatg atgataatcc caatgatctt
     4621 cttttgctat ttctcggatc gaatcaaatt cttttaatag tttgttgaaa cggcgatgaa
     4681 gttttttgaa ttcctttggt atagcttcct tacttcgttt cgttttttca taagatagtt
     4741 ctgccgaata catctctcgg gaacgattca ccaaattatg tgcttcgtca atcaaaaaca
     4801 gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa ataaacagtt ggatcaaata
     4861 aataattgta atccccaatg attacgtcac accagatact gacatctaat gatagttcga
     4921 aaggacaaag tgtatgcttt cttgcataag tttcgatgac ttcacgagtg atctgattct
     4981 catgattcaa aagatcccat aaaccttcat taatccgatt gtaataacca ttggcatatg
     5041 ggcactgatc tggattacag gtcgtctcat ctaaaaagca gatcttgtct ttcgccgtca
     5101 aagtgacact ttttacttca gctcctgtat cttttaatgc tgtcatagca tcttcagcta
     5161 cttgtctagt tattgtttta gccgttagat aaaagatctt ttctccctct tcttccccta
     5221 cagctttcaa agcggggaat aacgtagaga ttgtttttcc tgttccggta ggtgcttcta
     5281 caaacaattt ctgtttcgta cggatcgttt tataagcagc taccgctagt tcacgctgac
     5341 ctttcctata ctcatcatgg gggaatttca aagccatcaa agaggcattg cgtacagtgc
     5401 gccagttttc ttgaaaaaca agccattctt tataatcttc agtcagcttt ttaaaaaaag
     5461 tttctaactg ctctcttgtc tgatgttcaa cctttctcgt gatcacttct tctgccgtct
     5521 ggaagtaagt caattgcaga tttatttctt gtaaatcatg ttcttgacta tagatatagg
     5581 catacaccct tgcttgatga aagaaaagtt ccacttgttc tggttctaaa tcttcaaatc
     5641 gtggttcgga ggttttgatt tcatctatat aataaatacc gtctcttttg aaaataccgt
     5701 ctgcacggcc ttcaacgatc aggtaatatt catctaattg aactgttgtt tttaaaaaga
     5761 cttctttttc atagttttct cccgcatctt tttggagctt gcggtggatt ctcgctcctt
     5821 ctaatgcagt atggttactt tttttacgat tgtcgatact tccacgtcgc aaaataaatt
     5881 ctactaagtg ccgcacagat atttttgact gtttgatcat ttccaccacc tctttctctc
     5941 taagattacc tgaaaaaacc tagtttagca aaaagagctc tctttccaaa cataaaaaaa
     6001 caccctgctg attaggcagg gatataggat atagaatttg gatttttgga tgaatctaat
     6061 gtaacaagga catattaaaa tgctatgaaa acttactttc cttctcttta gaatatccaa
     6121 atacttttca tatagtctga taattcagat aagtaaagag aaataagtac ctgtttttgt
     6181 ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag ttttggctat caaggaggaa
     6241 tgcacatgca tatcgaaaga ttaaaaacag gaatgattga agaaaactgt tatttggtct
     6301 acaacgaaga agcacttttg atcattgacc ctggagaaga tgcggaacga atcaaacaag
     6361 aaatcaaaaa aacaaaccaa cagccggtag cgattttgct tacccatact cactacgatc
     6421 atatcggtgc agtggaacct ttacgcgcgt attatcaaat cccagtttat gtcagcccac
     6481 tagagcaaaa atggctaggt gatccgattt tgaatctttc tggactcggt agacatgatg
     6541 atattgctga tgttattgtc tcgcctgcag aatatgaatt tgaaatgaaa acttatactt
     6601 taggaaacat gtcttttcgc gtcgtcccta ccccagggca ttctatagga agcgtcagct
     6661 ttatttttga tgattttgtc gtttctggcg acgccctgtt tagaggaagt atcggtagaa
     6721 ccgatctgta tacagggaat ttagaacaat tacttcatag tattcggacc cagctgtttg
     6781 tattacctga tgaatttgca gtctatccag gacatggcga tgcaacgacg attgaacagg
     6841 aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt aggaggagaa taaaatgaca
     6901 gaaactacac tttatatcgt acgacacgga aaaacgatgt tcaatacaat tgaacgagta
     6961 caaggctggt gtgatacacc cctcacaaag caaggacaag aaggcattca ttatttaggt
     7021 cgcgggttaa aaaatgtcga cttttcattt gcttattcta gtgacagtgg tcgtgcaatc
     7081 gaaactgccc gtattattct aggagaacat tctaagggaa aagaaattcc ttattttatt
     7141 gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag gtggatatga catggaaatg
     7201 tggggtgtga tccctcgtgt attgaatttt aaaacatatg atgaaatgtt taccacagaa
     7261 gtttcttttg aacaaattgc taatgctgta tacaacttag ataccgctgg gtgggcggaa
     7321 acttatgaaa cattgcgaga ccgtgtatgg acaggatttg aagatatcgc ccatcactgc
     7381 gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg gtttgacgat cgcctttttg
     7441 cttaatctga tcgatcctaa acagcctgtt cgtgctgggc ttttaaatgg cagcgtaaca
     7501 aaggtcactt atgcaaacgg tcgattttct atccacggca tcaatgatac gtcttatatc
     7561 gataacggga aaacacatag tcgcgactcg tttctataat ggggatattt taatttttac
     7621 cagagaatca taaaaggcaa agtctgaacg agggctttgt cttctttttt ctcttcgtgt
     7681 acttatctgc tttatttctc aagtttttct taatattaaa aaacagattg ggtttctcaa
     7741 aaaataacgg tatactataa gatgtatctt aattgggaag gaaggaattt atgttttttg
     7801 caaatattat caaagcagtg attctcggta ttattgaagg aataacggaa tggctgccca
     7861 tcagtagtac tggtcatttg atcctagcag atgaattcat caaactggat gccagttcac
     7921 aatttatgtc gatgttcaat gtcgttatcc aattaggggc gatcttggct gtcgtcgttc
     7981 tttattttca caagttaaac ccattttctc caagtaaatc aacggtagaa aaaaaagata
     8041 cgtggacact ttggtttaaa gtgatcgttg cttgcatacc agccatcatc attggtttgc
     8101 ctttggatga ttggttagaa gcaaatttcc acaagttctt accagttgcc attgttttga
     8161 tcgtttacgg tattgccttt attattgtcg aaaaacgcaa caaaaacaaa acacctcgtt
     8221 ggacttctct gaatgacttt acgtaccaag cagctcttct tgttgggtta ttccaagtct
     8281 tggcacttgt tcccggaaca tcccgttctg gcgcaacgat cctaggatct atcttgattg
     8341 gtgcttcgcg ttttgtcgct acagagtttt catttttctt agggattcct gtcatgtttg
     8401 gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg gaattcattc aaaatggaag
     8461 aaactgttgt tcttcttgta ggaacattcg tatcttttgt ggtttccgtc ttagctatca
     8521 aattcttact aagctatttg aagaaaaacg actttaccgc ttttggctgg tatcgtatta
     8581 tccttggtat cattttgatt ggttattggt ttattgcaat gtagaaaaaa ccagaacact
     8641 tctct
//
LOCUS       DAHUWM010000115         8593 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHUWM010000115 DAHUWM010000000
VERSION     DAHUWM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.116"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            91..570
                     /locus_tag="NK788_001884"
     CDS             91..570
                     /locus_tag="NK788_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCH6770210.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            787..1878
                     /gene="phnW"
                     /locus_tag="NK788_001885"
     CDS             787..1878
                     /gene="phnW"
                     /locus_tag="NK788_001885"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCH6770211.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1871..1999
                     /locus_tag="NK788_001886"
     CDS             1871..1999
                     /locus_tag="NK788_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCH6770212.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            2003..2548
                     /locus_tag="NK788_001887"
     CDS             2003..2548
                     /locus_tag="NK788_001887"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCH6770213.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(3002..4693)
                     /locus_tag="NK788_001888"
     CDS             complement(3002..4693)
                     /locus_tag="NK788_001888"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCH6770214.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5113..6060)
                     /gene="arcC"
                     /locus_tag="NK788_001889"
     CDS             complement(5113..6060)
                     /gene="arcC"
                     /locus_tag="NK788_001889"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCH6770215.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6175..7194)
                     /gene="argF"
                     /locus_tag="NK788_001890"
     CDS             complement(6175..7194)
                     /gene="argF"
                     /locus_tag="NK788_001890"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCH6770216.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7285..8514)
                     /gene="arcA"
                     /locus_tag="NK788_001891"
     CDS             complement(7285..8514)
                     /gene="arcA"
                     /locus_tag="NK788_001891"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCH6770217.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2462 a         1741 c         1590 g         2800 t
ORIGIN      
        1 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
       61 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
      121 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
      181 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
      241 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
      301 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
      361 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
      421 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
      481 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
      541 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
      601 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
      661 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
      721 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
      781 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
      841 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
      901 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
      961 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     1021 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     1081 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     1141 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     1201 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     1261 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     1321 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     1381 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     1441 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     1501 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     1561 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     1621 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     1681 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     1741 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     1801 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     1861 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     1921 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac
     1981 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     2041 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     2101 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     2161 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     2221 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     2281 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     2341 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     2401 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     2461 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     2521 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     2581 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     2641 atgaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     2701 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     2761 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     2821 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     2881 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     2941 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     3001 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     3061 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     3121 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     3181 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     3241 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     3301 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     3361 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     3421 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     3481 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     3541 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     3601 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     3661 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     3721 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     3781 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     3841 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     3901 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     3961 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     4021 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     4081 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     4141 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     4201 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     4261 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     4321 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     4381 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     4441 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     4501 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     4561 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     4621 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     4681 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     4741 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     4801 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     4861 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     4921 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     4981 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca
     5041 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     5101 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     5161 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     5221 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     5281 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     5341 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     5401 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     5461 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     5521 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     5581 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     5641 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     5701 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     5761 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     5821 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     5881 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     5941 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     6001 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     6061 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     6121 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     6181 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     6241 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     6301 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     6361 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     6421 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
     6481 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
     6541 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
     6601 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
     6661 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
     6721 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
     6781 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
     6841 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
     6901 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
     6961 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
     7021 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
     7081 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
     7141 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
     7201 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
     7261 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
     7321 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
     7381 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
     7441 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
     7501 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
     7561 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
     7621 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
     7681 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
     7741 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
     7801 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
     7861 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
     7921 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
     7981 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
     8041 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
     8101 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
     8161 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
     8221 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
     8281 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
     8341 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
     8401 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
     8461 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
     8521 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
     8581 ttttgtctca ttc
//
LOCUS       DAHUWM010000116         8572 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHUWM010000116 DAHUWM010000000
VERSION     DAHUWM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.117"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="NK788_001892"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="NK788_001892"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCH6770218.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="NK788_001893"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="NK788_001893"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCH6770219.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="NK788_001894"
     CDS             complement(3070..4323)
                     /locus_tag="NK788_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCH6770220.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="NK788_001895"
     CDS             complement(4329..5252)
                     /locus_tag="NK788_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCH6770221.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="NK788_001896"
     CDS             complement(5473..7965)
                     /locus_tag="NK788_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCH6770222.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="NK788_001897"
     CDS             complement(7972..8439)
                     /locus_tag="NK788_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCH6770223.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAHUWM010000117         8519 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHUWM010000117 DAHUWM010000000
VERSION     DAHUWM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.118"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            52..339
                     /locus_tag="NK788_001898"
     CDS             52..339
                     /locus_tag="NK788_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770224.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            755..2293
                     /locus_tag="NK788_001899"
     CDS             755..2293
                     /locus_tag="NK788_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCH6770225.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2290..3255
                     /locus_tag="NK788_001900"
     CDS             2290..3255
                     /locus_tag="NK788_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770226.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3242..4564
                     /locus_tag="NK788_001901"
     CDS             3242..4564
                     /locus_tag="NK788_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6770227.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4627..4731
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4805..5548
                     /locus_tag="NK788_001902"
     CDS             4805..5548
                     /locus_tag="NK788_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770228.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5563..6555
                     /locus_tag="NK788_001903"
     CDS             5563..6555
                     /locus_tag="NK788_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6770229.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6567..7304
                     /locus_tag="NK788_001904"
     CDS             6567..7304
                     /locus_tag="NK788_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770230.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7294..8016
                     /locus_tag="NK788_001905"
     CDS             7294..8016
                     /locus_tag="NK788_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCH6770231.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8100..>8519)
                     /locus_tag="NK788_001906"
     CDS             complement(8100..>8519)
                     /locus_tag="NK788_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCH6770232.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2749 a         1463 c         1836 g         2471 t
ORIGIN      
        1 aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca
       61 gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt
      121 ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa
      181 tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt
      241 ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt
      301 tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac
      361 gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt
      421 ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg
      481 gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag
      541 tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg
      601 atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt
      661 tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg
      721 tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag
      781 tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc
      841 tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa
      901 aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt
      961 attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt
     1021 tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc
     1081 gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt
     1141 agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat
     1201 catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt
     1261 gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt
     1321 gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc
     1381 catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc
     1441 cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct
     1501 gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc
     1561 gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta
     1621 tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag
     1681 tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt
     1741 cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag
     1801 atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca
     1861 gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca
     1921 agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca
     1981 agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa
     2041 ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc
     2101 accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa
     2161 attaagcgga aaaataacgg aggatcaaat gattgatatg aattaccaag tcaatgtcga
     2221 aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga
     2281 ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag
     2341 tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt
     2401 ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca
     2461 gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg
     2521 tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa
     2581 aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta
     2641 gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga
     2701 gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca
     2761 gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta
     2821 caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac
     2881 aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt
     2941 atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat
     3001 ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga
     3061 tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg
     3121 aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga
     3181 agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga
     3241 aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta
     3301 tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac
     3361 ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa
     3421 tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga
     3481 acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt
     3541 agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat
     3601 tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc
     3661 tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt
     3721 gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc
     3781 tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt
     3841 aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat
     3901 tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt
     3961 agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc
     4021 gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc
     4081 agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc
     4141 aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg
     4201 gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc
     4261 cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt
     4321 ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa
     4381 ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca
     4441 agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga
     4501 gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa
     4561 ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa
     4621 aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac
     4681 ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg
     4741 ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa
     4801 caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca
     4861 gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt
     4921 ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga
     4981 agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga
     5041 ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc
     5101 aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt
     5161 gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag
     5221 gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat
     5281 ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc
     5341 tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt
     5401 agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat
     5461 catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg
     5521 gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt
     5581 ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa
     5641 caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat
     5701 catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc
     5761 caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca
     5821 caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata
     5881 ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca
     5941 gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt
     6001 ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa
     6061 ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc
     6121 caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac
     6181 tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg
     6241 aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa
     6301 aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat
     6361 gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat
     6421 acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct
     6481 tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat
     6541 cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca
     6601 ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat
     6661 cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc
     6721 agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag
     6781 catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg
     6841 ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc
     6901 tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga
     6961 tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg
     7021 atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga
     7081 agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa
     7141 tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga
     7201 ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc
     7261 aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta
     7321 gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat
     7381 tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc
     7441 ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct
     7501 tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta
     7561 ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag
     7621 aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct
     7681 taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca agactatatt
     7741 gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa
     7801 ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca
     7861 gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag
     7921 ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat
     7981 caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg
     8041 tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct
     8101 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
     8161 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
     8221 gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
     8281 gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt
     8341 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga
     8401 accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga
     8461 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DAHUWM010000118         8494 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHUWM010000118 DAHUWM010000000
VERSION     DAHUWM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.119"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..173)
                     /locus_tag="NK788_001907"
     CDS             complement(<1..173)
                     /locus_tag="NK788_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770233.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="NK788_001908"
     CDS             complement(593..1714)
                     /locus_tag="NK788_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCH6770234.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="NK788_001909"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="NK788_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCH6770235.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="NK788_001910"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="NK788_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCH6770236.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="NK788_001911"
     CDS             complement(4304..5215)
                     /locus_tag="NK788_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6770237.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="NK788_001912"
     CDS             complement(5232..6239)
                     /locus_tag="NK788_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCH6770238.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="NK788_001913"
     CDS             complement(6236..8365)
                     /locus_tag="NK788_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6770239.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="NK788_001914"
     CDS             complement(8370..>8494)
                     /locus_tag="NK788_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6770240.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAHUWM010000119         8430 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHUWM010000119 DAHUWM010000000
VERSION     DAHUWM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.120"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            219..1742
                     /locus_tag="NK788_001915"
     CDS             219..1742
                     /locus_tag="NK788_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCH6770241.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1979..3319
                     /locus_tag="NK788_001916"
     CDS             1979..3319
                     /locus_tag="NK788_001916"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCH6770242.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3472..4209
                     /locus_tag="NK788_001917"
     CDS             3472..4209
                     /locus_tag="NK788_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCH6770243.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4374..4595)
                     /locus_tag="NK788_001918"
     CDS             complement(4374..4595)
                     /locus_tag="NK788_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCH6770244.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4626..5657
                     /locus_tag="NK788_001919"
     CDS             4626..5657
                     /locus_tag="NK788_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770245.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5644..7047
                     /locus_tag="NK788_001920"
     CDS             5644..7047
                     /locus_tag="NK788_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCH6770246.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            7113..7748
                     /locus_tag="NK788_001921"
     CDS             7113..7748
                     /locus_tag="NK788_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6770247.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7797..>8430
                     /locus_tag="NK788_001922"
     CDS             7797..>8430
                     /locus_tag="NK788_001922"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCH6770248.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVV"
BASE COUNT         2791 a         1436 c         1807 g         2396 t
ORIGIN      
        1 aagacgaaaa tgtttgttta taaaaaaaag gaaacgctac aatgaaaaag aagaaagctt
       61 taaaaaactg aaaaaagagg agggaatagg gaaaaacgaa ctttttgaac aaaagtttta
      121 caaaatgcgt gttattattt ttgttctgat actctttttg ataaaataac attagagcca
      181 aaattgttgt ctctaacgga aaagaaagga agaagaagat ggaacaaaca aaaaagaaaa
      241 aatttcaaat gccttcagcg tatacggtct tatttattat tattgcagta atcgctgtac
      301 ttacttggtt tataccagct gggcactata gtgtagacga tgcaggcaac atcgtcgctg
      361 gatcatatga acgagtacaa caaaatcctc aagggctgtg ggatattttc atggcgccaa
      421 tcaatgggat gctaggtgta ccagcggggg agctaactaa agaaacacct gctgcaatcc
      481 caatctcttt cttcattttg gttgttggtg gatttttagg tattatcaac aaaacagggg
      541 cactagatgc cgggattgct tcagttgtga agaaattcaa aggaaaagaa aaaatgctta
      601 ttccagtatt gatgctgtta tttgcattag gcggctctac ttacggaatg gcagaagaaa
      661 cgattccatt ctatgcttta ctgatcccag tcatgatggc tgtaggcttt gatacagtag
      721 tagctgtagc aatcgtatta gtagggtctc aagtaggctg tctagcttca acggtaaatc
      781 cttttgcaac aggcgtagct tcacaagctg ttgggatttc gatgggtgaa ggtattggtc
      841 ttcgattctt gatgttcgtt attttgttag ctatttctat tgtatatgtg tatcgttatg
      901 catcaaagat cgaaaaagat ccaacaaaat cattagtata caaccaacgt aaagaagata
      961 tgaagcattt cgatattgaa gcaatcggtc gtcaagaagt aatcacgaaa aaacaaaaac
     1021 atgttaatgt attgttcttt attacttttg gtatcatgat catcagtttg attccttgga
     1081 caaccttgaa tgcgaatttc acgatttttg aatcattgac aaactggtta acaaaccttc
     1141 cggtactagg tacggttcta ggtaaaagca tgcttccatt aggtgactgg tacttcccag
     1201 aaattactat gttattctta gtaatgtcca tcgtagtagc aatcacttat ggcatgaaag
     1261 agtctgaatt tgtctctgaa tttttagctg gtgcttctga tttgatcggt gtagcaatcg
     1321 tagtagctgt tgctcgtggt atccaagtgg ttatgaacaa tggattaatc acagacacca
     1381 tcttacattg gggcgaacaa ggattaagtt cattatcttc aagtgtattc atcattatta
     1441 catttatttt ctatatccct atgtcattct tgattccttc aacttccggc ttagctgctg
     1501 caacaatggg aatcatggga cctatgggtg aatttgccgg cgtcggtaag gatttagtaa
     1561 tcactgctta tcaatctgca tctggtttag taaacttaat tacaccaaca tctgctatcg
     1621 taatgggtgc tgtagcaatt gctcgtttcg atgtttcagt gtggtggaaa tttaccggta
     1681 aattaattgc gatattattt gtagccatct gtgtgatcct aggtgtagca gcaatgttct
     1741 aaacctagac aaatacaggt acaaggtcta tcataagaat gaagaaaccg ataaacggct
     1801 atcgataagc acgtggacaa gtcaatagct ccattcagtg agtgtttttt ctttcatcat
     1861 ttgttaagaa cattctgctt gctgatggag caggcttaaa acgtcattat ttgagaagaa
     1921 gaggttgtgt cggaggaatt tgacatgacc ctttttaaca tattaggagg ataatatcat
     1981 gaaaaaattt gtgacagaac aacatcatga acaagcatta gaatcattga gcgaattgat
     2041 ccgaattcct tccgtactag atgaagcaga ttcaggacaa ggacatccct tcggtaaaaa
     2101 agtgattgaa gctttggata aagtattgga aatcagtgaa aagctaggat ttagaacatt
     2161 taaagatcca gaaggctatt acggttattc agagattggc tctggagatg aattattcgg
     2221 tatcttgtgt catatggacg ttgttccagc aggggacgaa aataattggg aaacaaaacc
     2281 attcgatcca actataaaag acggctggtt agttggtcgc ggatcccaag atgacaaagg
     2341 tccttctatt gcggcaatgt atgccgtcaa agctttgatt gatgctggcg tggaattcaa
     2401 gacacgtatt cgttttatct ttggtacaga tgaagaaaat ttatggcgtt gtttagaaaa
     2461 atataacgaa aaagaagaag gaattacgca aggatttgcg ccagatgctg aatttccttt
     2521 aatttatgca gaaaaaggct tattgcaagc ttacttgaca ggaccaggaa cgaacgagtt
     2581 ttctgtaaaa gctggaggag cgttgaatgt tgttcctgat gcagctcctt actctggaga
     2641 aaagctttct gaagtaaaag aagcattgaa aaaacacgaa ttcgatttcg aagaacaagg
     2701 tgaaggcatc gtcgttcttg gtaaaagtat ccacgcgaaa gatgcagcac aaggagtgaa
     2761 tgctatttct cgtctggcaa ttgctctttc agaagtattt gattttggcc caatcaattt
     2821 cttaggaaaa ttagttcaag aaaatgcaac tggtgaagct gtcgtgggca aaacagaaga
     2881 tgaacaatcc ggcgagctaa cgatgaactt tgcaagtttg gaaatcacac cagaacaaac
     2941 aaaaatcggt gtagatatga gaatccctgt tacattcaag aaagatgatt tagtagctaa
     3001 attaactaaa acagcagaaa aatatgggtt gacttatgaa gaatttgact tccttgattc
     3061 attatacgtg ccactagaca gtgagttagt gaaaaatcta ttggggacat atcgcgatat
     3121 tactggagac atgactgaac cgtttgtatc aggcggcgct acttttgctc gtacgatgaa
     3181 ccagtgtgtg gcattcggtg cgatgttccc tgatactcct gactttatgc accaggcgaa
     3241 tgaacgatgg gaattaagta gtatgtataa agcaatggaa atttatgcag aagctgtgta
     3301 tcgtttatgt gctaaataaa aataaacata caaaaagggc ggatattcaa atggatattc
     3361 cgtctttttt tatttgatgc tggaaagttt gcatttctct cttatttttt taaggtataa
     3421 tcatacttga ttaagaaaaa ggggtcactc agatagaaaa ggtgggagaa agtgaaaata
     3481 tttagacgaa tactaattgg cttagtcatc attttaggat tactcggtgg agccggttat
     3541 tactatatac agcaaaatac gtacgaacca tcgtcagaag cgcaagtgtt ggtaaaagaa
     3601 ggaaaaaaca aagaagatta cttatttttt gaaggagaga aaaagaatca tggaaagtct
     3661 ccattactta ttttttatcc tggtgctctc gtagagccag aaagttatag tatttgggcg
     3721 caagatttag caaaaaacgg cttttcagtc gctattgtca aaatgccact tgatctagca
     3781 gtgatgggcg gaaacaaagc ggataaggtc aaagaagatt ttccaaattc cgattatgtg
     3841 atcggtggcc attcattagg tggcgtaatg gcaagtcgtt atgtcaataa gaatcattca
     3901 gacaaacatt taaaaggggt attcttcatg gcaagttatc ccgacaaaaa aggaacattg
     3961 acagaatcag ctgttcctgt tttatcgatc acggcgacaa aagataaagt attgaatcag
     4021 gaagcttatc aagaagctaa gcagtattta ccggaaagaa cagagtatgt atcaatcaat
     4081 ggcggaaatc atggaggttt tggcagctat ggtgagcaaa aaggagatgg agaggcagaa
     4141 atttctaacg cagaccagca aactgtgatc gtcaatacat tggaaaactg gctgaaaaag
     4201 ttgaaataat ataaatgggt cctatagggt ccgtgaaaaa actagtcaat taactataga
     4261 tagcaaatga atctttcaaa ataagtcggg atttcacgga acaagagaaa cagatcccgg
     4321 cttattttca agtttagaga acttagtgta cgtttttaaa gtttcgttta tatttatatt
     4381 ttattttcta ataaaatcgc tcgaactgga catttttgat acgctttttt tacatatgtt
     4441 tcttcttccg gcatcacgaa ctgttgatga gcctctggtt cttgggcaaa taaaacgatt
     4501 ccttcttcat cataatcgaa aatatctggc gcttcgattt gacaaagacc gcatgcaata
     4561 cagcgttcag gtacaagttt acatagtagt gacatttcta accatcctaa cttaaggaga
     4621 ataatatgat tgtttttatt ttatcgctat tttcatcagg acacaagctg cggattagtt
     4681 ctctttacca actccttgtg ggaaaacgaa cgacttcagt acttatttat ggatttaccc
     4741 acgaactttt gtttatccat aacagttttc cagaattgaa gcaggataag ttttatcaaa
     4801 ttttgcaaaa aattgcgcag caaggttgga tcgaaataaa tgaaaatgaa gcaaagctaa
     4861 cgcctgcagg ggctgatatg ttgtccgaac atcaaataga acatactggt ttgcgttttg
     4921 accgctatgg cagaacaggg gaaactagtt ggcggctcat taaatttgct gttcaagtaa
     4981 tttctaatct ggcaacgggt attcaagact atcttccagc agagacaagt cctttttata
     5041 ctttccaact aaaaaaatgg cttagtgaaa gccaattgcc acgtgaaatt ttgattgata
     5101 ctgcgtatga gagtttggtg cagctgtttt cggagatacc agaagaagca gcagattttt
     5161 tagctaacca attatctgga aatgagcgaa cagggctgct tccttatcag ctggcaaaaa
     5221 acaatgatga gtcagaggtt tatctgcaac aaagcagatg tatccacctt ttgttagcac
     5281 aaatcgaaaa acggccagat tcgctttggc acgctttgat cgattcatta ttacagcaaa
     5341 atttcaatca aagcatgatg atcaccaagc agatgtttat gaatggtcag acaattgacc
     5401 agatcatggc aatccgtcat ttaaaaagag gaacagtaac agatcatctt attgagtggg
     5461 ccttgttttt tgatgatttt ccatacgagt ggattctctc tgcagaaaca atcgaacgtt
     5521 tggaaccaaa taaggattcc gtgcgagagt ggcgctttag cgaatggaat gtagatggac
     5581 aattagatta tggagaattt cgtttgtatc aaatctatct attgagaaag gaagcgatac
     5641 agaatgttaa taaatgaatt aaaaaaatat tttggatttg attcatttcg tcccggccaa
     5701 gaagaaatta ttcaggcgtt gcttgatgga gaagatactc tcgctatttt gcctacagga
     5761 acgggaaaaa gcctttgcta tcaattgacg gggtatttaa tggaaggtct ggtcatcatc
     5821 gtgtcaccgc tgctttctct aatggaagat caagtgacgc aattacaaaa acgaggggag
     5881 aaacgagtag ctgctttcaa tagtttactt tcgcaatctg aaagaagata cgtcctccgt
     5941 catctatcgc aatataaatt tttgtttttg agtcccgaaa tgttgactaa tccttctctt
     6001 ttagagcatc tgaaaaaaca aaatattgcg ttatatgtag tagatgaggc tcattgcgtg
     6061 tctcaatggg gagttgattt ccgtccagaa tatcaacagt taggaaaaat ccgaaagctt
     6121 ttaggaaacc cggtcacact ggcactaacg gcgacagcaa cagatttagt agcaagagat
     6181 atacgtcatg tactatttga tcgaccacca aaagagatca gacaatcagt caatcggcaa
     6241 aatatctcgc tttttgttcg gaaaacgcag caaaaagaac aagaacttga acacttcatg
     6301 gaaagagcac aaggagcggc aatcatttat tgtgcaacaa aaaaagaagt cgaacgattg
     6361 tatcatttat tcagggaacg ttttacagtt ggatactatc acggtggtct tgatgcagct
     6421 caaagaagac aacttcaaca gcagtttgta aaaaatcagc tgcagtttct cattgctacg
     6481 aatgcatttg gtatggggat cgataaatct gatatccgtt atgtggtcca ttatgatttg
     6541 ccagacagtc tagaaaacta tgtacaagaa atcggtcgag caggcagaga tcaggaagaa
     6601 agtgcagcaa ttctttttta tcaaagtggg gatgaacgta tccattattt ctttaatcaa
     6661 ctttctagag aacagcgaca aagttttgag ctttatttgg aatatgccac aaagcaagct
     6721 ccctttgatg agctgcaaaa aaaatggatg gagctgatcc agcagtctga aaaaccagaa
     6781 aattgggtgg aacggctcaa acgtcaggaa aaagaaaaag aattccgcct gcagcagatg
     6841 cttcgctata ttaatgaaga aaactgccgc agaaaattca tcttagctta ttttggcgaa
     6901 aaactctccg aaaagccgca gaattgttgt gatattgatg gagcaaaagc agaacttctg
     6961 ccaaagagga agcttttttc taagactgaa caacttcatt gggaaacgat tttactaaat
     7021 ttatttaaaa aaaacatgga acattgattt tttaggacaa gacacttttt cctatggtat
     7081 aatacacacg atagagttta ggaggtaaaa cagtgagcag aaaagataga catcaatcta
     7141 cagaaaataa cgaaacacaa gaaccatggg aacaaccaat ctacgataca gatgatgaag
     7201 tttcatcaag aagttctcag agacaacaaa agaaaggaaa tacattattt ctttcgctat
     7261 ttttgatttt actggtctta tgtatcgcta ttccagcagg tgcttatttc tggatcagaa
     7321 atggatcaag caacaatgca acagcagcaa cttcttctga aacttcgaca tcagtagtag
     7381 aaagttctac aagtacaaca aaagaggagt caactgagac ttcaacagta gaatctagta
     7441 caatcgcgga agaatctact gtgtctcaag atacaggtgt aacgtcagaa agcagtattg
     7501 cagcagagac aacaccgact tcctcaagtg aggcaactta tcaagcaggg gattcaagca
     7561 gcaatgcagc agaaacaaca ccttcttcat cagctgcagc cggttcaaca acgactgttc
     7621 aagcaggcga aggtccaaaa caagtggctg agcgtgcagg gatcaccact gatcaattgt
     7681 ttgaattgaa tggcttagat ccaaacaact ttatgcttta ccctggtcaa gaattaagaa
     7741 ttaaataata atagccgcgc tttgcggctt tttttctgag aaaaaggagt tatatcatga
     7801 cacaaatcag tattgcaatc gacgggcctg cttcatctgg gaaaagtacc gttgccaaga
     7861 tattagcaaa agaattgaat tatatttata cagataccgg tgccatgtat cgcgcagtaa
     7921 cttatttagc tattacgaac aatgtatctt tttcagatga agaagggcta gtttcgctga
     7981 tcaaaaagta tcctatctct ttttcgcaac aagaagatgg acagcatgta ttcatagcat
     8041 caaaagatgt gactttagaa atccgtcaac cagatgtcac aaaagctgtt tctgaagtct
     8101 ctgcacatgg aaaagtcaga gaagaattag ttgctgtcca acgaaaaata ggggaacgcg
     8161 gtggcgttgt catggacgga agagacatcg ggacagctgt attaccgaat gcagaagtga
     8221 aaatcttttt agtagcaagc gtaagtgagc gtgcagaaag acgtttcaaa gagaaccaag
     8281 aaaaaggaat tccgactgat cttgaaacac tgacgaaaga gatacaggaa agagaccatt
     8341 atgattcaac aagggacgtc tctccgctga agcaagcaga ggatgctgtt cgtatcgata
     8401 cgactggcaa atcgattcca gaagtcgttg
//
LOCUS       DAHUWM010000120         8360 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHUWM010000120 DAHUWM010000000
VERSION     DAHUWM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.121"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            205..684
                     /locus_tag="NK788_001923"
     CDS             205..684
                     /locus_tag="NK788_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770249.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="NK788_001924"
     CDS             717..2945
                     /locus_tag="NK788_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6770250.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="NK788_001925"
     CDS             3074..4717
                     /locus_tag="NK788_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCH6770251.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="NK788_001926"
     CDS             complement(4790..5335)
                     /locus_tag="NK788_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCH6770252.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="NK788_001927"
     CDS             complement(5358..5843)
                     /locus_tag="NK788_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6770253.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="NK788_001928"
     CDS             complement(5833..6645)
                     /locus_tag="NK788_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCH6770254.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="NK788_001929"
     CDS             complement(6784..8316)
                     /locus_tag="NK788_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCH6770255.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         2819 a         1591 c         1646 g         2304 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcag cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat
//
LOCUS       DAHUWM010000121         8185 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHUWM010000121 DAHUWM010000000
VERSION     DAHUWM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.122"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            13..543
                     /gene="lepB"
                     /locus_tag="NK788_001930"
     CDS             13..543
                     /gene="lepB"
                     /locus_tag="NK788_001930"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCH6770256.1"
                     /translation="MNRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSMENVLKQGD
                     MVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKVVKEPYLT
                     KNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTINKTDILG
                     KARFVYYPLDEIKWIQ"
     gene            complement(626..1126)
                     /locus_tag="NK788_001931"
     CDS             complement(626..1126)
                     /locus_tag="NK788_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCH6770257.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1405..1803)
                     /locus_tag="NK788_001932"
     CDS             complement(1405..1803)
                     /locus_tag="NK788_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6770258.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2063..2923
                     /gene="ylqF"
                     /locus_tag="NK788_001933"
     CDS             2063..2923
                     /gene="ylqF"
                     /locus_tag="NK788_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCH6770259.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            2916..3683
                     /locus_tag="NK788_001934"
     CDS             2916..3683
                     /locus_tag="NK788_001934"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCH6770260.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3739..4596
                     /gene="dprA"
                     /locus_tag="NK788_001935"
     CDS             3739..4596
                     /gene="dprA"
                     /locus_tag="NK788_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCH6770261.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4718..6796
                     /gene="topA"
                     /locus_tag="NK788_001936"
     CDS             4718..6796
                     /gene="topA"
                     /locus_tag="NK788_001936"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCH6770262.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6765..8141
                     /gene="trmFO"
                     /locus_tag="NK788_001937"
     CDS             6765..8141
                     /gene="trmFO"
                     /locus_tag="NK788_001937"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCH6770263.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2851 a         1444 c         1720 g         2170 t
ORIGIN      
        1 aaacaacgct atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc
       61 cttgcgttca tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg
      121 gaaaacgtgt tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga
      181 tttgatattg ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt
      241 cttccaggag aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt
      301 aaggaacctt atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg
      361 gactttacac tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta
      421 cttggagata atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact
      481 gatattctag gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag
      541 tgagaaacaa taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat
      601 cagtttacta ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta
      661 aagttttgat aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga
      721 tgaatctacc tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag
      781 aattgataaa aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca
      841 ggtttatctt tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga
      901 taagaaaagc agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca
      961 acatttcaag caaaccagcc cagatcggtc catatagata acccataagc gccatactta
     1021 aaaatccgaa tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat
     1081 caagggctaa taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa
     1141 aaacgtcttt tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa
     1201 ggataccata aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct
     1261 aacaattaaa aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga
     1321 ataaaaaaaa tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata
     1381 aaaaacgtca gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc
     1441 gtctagatct tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc
     1501 gtggaaagta ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt
     1561 ttcaacacta aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc
     1621 ttttataaat tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca
     1681 taccattgaa ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc
     1741 ataaaaagca acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt
     1801 catttgtttc gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgctgt
     1861 caaaaattta gattcatata tagaaaccaa aataaaaatt tgcatcatca aaaaagtgaa
     1921 gatgtgatag aatgaggact ggttcacttt cacttaacta atagatggaa tttctttata
     1981 ataaaaaagt gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg aatattccgg
     2041 tatgaaagga atggagagaa caatgaccat ccaatggttt ccgggacata tggcaaaagc
     2101 ccgacgagaa gtttctgaaa aaatgaaata tgtggacatc gtatttgaat tggtagacgc
     2161 acgattacct ttatcttctc gtaatccaat gctagatcag atcattcagc aaaaacctcg
     2221 ccttgtttta ttgaataaag cggatctggc agatccacaa cagacaaagc tgtggcaaga
     2281 atattttcgt aaacaaggcc atcttgcatt agctatcacg gcacaagaaa gtaaaggaat
     2341 taaaggtcta gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa gagatgctgc
     2401 tcgggggatc aagcctcgcg ctattcgggc aatggtctta ggtattccaa atgttgggaa
     2461 atctacgttg atgaatcgac tagtaggcaa gaaaattgca cagacaggaa ataagcctgg
     2521 cgtaacaaaa ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt tagacacacc
     2581 agggatttta tggcccaaat ttgaagatga agaaatcggc aaaaaactag cgctgacagg
     2641 cgcgatcaaa gaccagcttg tccatttaga tgatttagcg atttacggat tagacttttt
     2701 tgcccgatat tattcaggaa gaatcagcga tcgttatggt ttatcaatcg agcaagaaga
     2761 aaaattggca ccggaattac tgatggatat tacagcaaaa cgtgggtata aagaagacta
     2821 tgatcgtggc agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg gacgatacac
     2881 actggatcgt tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc caaagtatca
     2941 aagaacgatt gaaaagcata cagagaatag atgatcctac actagaatca ttaagaaatg
     3001 atccacgcaa aggcgtgcag caagcactgg cttcttttga acgacgattg aaaaaggaag
     3061 cattaattaa ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg tacgcacaag
     3121 gtcaccaatg gattgccggc attgatgaag taggaagagg accattagct ggacctgtcg
     3181 tggcagctgc agttatcttg ccgaaaaatg agatcatatt agggctgaat gactccaaac
     3241 aattatcaga gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa gccctggcaa
     3301 tcgggatcgg gatcgttgat cagcagacaa tcgatgatgt gaatatttat gaagcaagta
     3361 aattagcgat gatccaagca gtagaaaaat tgtccattgt accaagctat ttgttgatcg
     3421 atgcgatgac cttggatttg cctattcagc aggaaaaaat aatcaaaggg gatgcacgat
     3481 ctgtgtcgat tgctgctgca agtatcattg ctaaagtata tcgagaccaa ttgatgaaag
     3541 aatatgacgt gctttatcct ggatatggtt tctcgcataa tgcaggttat ggaacaaagg
     3601 aacacttaga aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa agttttgcac
     3661 ctgtaaaaaa atatttacaa taaaaaacag ccgcttttac gtatcttatt agaaagagag
     3721 acgaaaggcg gttttatgat gtatcaaata gaagaaaatt tattgaaact tgcttataca
     3781 aaaggaatca gtagtcaagg aaaatgggca cttgctaact ggatgatgca gtatccttat
     3841 aaagaaatcg gatttgaaga catcattaag ataggtaaaa taaccaaaca tcgggagctg
     3901 tttcgcaaaa gttggcagga aattcacgct aactggggca aaatccaatc gaaacagtcg
     3961 tttatcactt gctttgatcc caaatatccc cctcagctgc ttcacttgac ctatccgcca
     4021 attgtactgt tttataatgg tgatctatct ttactttctt gtaacatgtt aagcgttgtt
     4081 ggtggaagac acacttctgc tttagccaaa aaaacagttt attatttact aaaaccagtg
     4141 atagaagctg gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac atacggtcac
     4201 caagcagcaa tctctcatgg tggacggaca atcgcagtca ttggaacagg gatagagcaa
     4261 gtctatcctg ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca tcttcttttg
     4321 agtgaatatc ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag aaatcggatt
     4381 atcgcagctt tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag cggctctctg
     4441 ataacagcac agcaagcatt ggatcttgga agaacagttt tttctgttcc aggaaatatt
     4501 ttaacagaac attctaccgg atgtcatcat ttgatccaag acggtgcagt ttgcacggtt
     4561 tcgggtgaag atatcttggc agaatttaaa gaataacacc atacttttca tttgcttttc
     4621 ttgacaaacg ttttctgaat ggatatgatt agctacgatt tgtatgaatc ctcttttaat
     4681 agtaggaaca cgacaatgga aaggagtcca ttcagtaatg gcgtataaat atttagtgat
     4741 tgtagaatca cctgcaaaag ccaagacaat tgagaaatat cttggacgaa actataaagt
     4801 aatggccagt gttggccata taagagattt accgaaaagt aaaatgggta tcgattttga
     4861 aaacaattat gaaccccatt atatttctat acgcggaaaa ggcgatgtca tcaaaagcct
     4921 gaaagccgca gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg atagagaagg
     4981 agaagcgatt gcttggcatt tagcgtacct acttgggttg gatctgaaag aaaaaaatcg
     5041 ggtggtcttc aatgaaatca caaaagacgc agtcaaagca gcttttaagg aaccaagaac
     5101 gatcgatgta gatttagtag atgcacagca agctcgtcgt accttagaca gaatcgttgg
     5161 ttattcgatc agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg caggacgtgt
     5221 ccaatctgtc gctttaaaaa ttattattga ccgtgaaaaa gagatccgag aatttgttcc
     5281 agaagaatat tggagcatcg acggtaattt taaaaaagct cgcaagaaat tcaaagcaaa
     5341 tttctgggga atcgacggta agaaaaagaa attaccagat gcacaaagtg taaaagaagt
     5401 cactgctaga attgatggaa cagacttcga agttaaaaaa gtagaaaaga aagagcgcaa
     5461 aagaaaccct gcgaatcctt tcactacaag tagcctgcaa caagaagctg cccgtaagtt
     5521 aaactttcga acaagaaaaa ccatgatggt agcacagcaa ttatatgaag gaatcacttt
     5581 gggtaaacaa ggaacagtcg gcttgattac atacatgcgt actgattcta cccggattgc
     5641 cgatacagca aaagcagagg ccgcagcatt tattgaagat acgtatggaa aagaattttc
     5701 tagacatgat caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc atgaagctgt
     5761 gcgtccaacg agtgtgcttc gtacaccaga tgaactcaaa caatatttag ataaagatca
     5821 attgaaactt tatacattga tctggtcacg ttttgtggct agtcagatga caccagctat
     5881 tttagataca atgaaagtga cattagaaca aaatggtgtc atcttcattg cgaacggttc
     5941 aaaaatcaaa ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg acggcaaaga
     6001 agagaaagaa aatattcttc cagaacttga agaaggcgat gtagtccaat ctgttgatat
     6061 tgaaccaaaa caacatttta ctcaaccacc agcacgtttt agtgaggcta cgttgattcg
     6121 tgcactagaa gaaaacgggg tagggcgacc ttccacttat gcaccaacac ttgatacgat
     6181 ccaaagacgc tactatgtaa aattgacaca aaaaagattt gagccgactg aactaggaga
     6241 aattgtcaat tcattaatct gtgaattctt ccctcaaatc gtcgatatcc atttcacagc
     6301 agaaatggaa ggagatttgg ataaaattga agaaggcacg gaagcgtggg tcaaagtggt
     6361 tgatcgtttc tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa aaatcgaaaa
     6421 aattcaaatc aaagatgaac cagcaggatt cgattgtgat gtctgtggac atccgatggt
     6481 gataaaatta gggaaatatg ggaagtttta tgcttgcagt aattttcctg attgtcggaa
     6541 tacgaaacca atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg aaggacaagt
     6601 cattgaacga aaatctaaga aaaacagact attctatggt tgttcccgtt tcccagaatg
     6661 tgaattcact tcttgggata aaccagtggg cagaaattgt ccaaaatgtg acggatatct
     6721 ggtcgagaag aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag attacgaaga
     6781 agacatccaa aaataataga aaagaggttc tttcattgaa aatggtaaat gttgttggag
     6841 cagggcttgc cggaagtgaa gctgcttggc agatcgcaca agcaggagtc cctgtccgtt
     6901 tatatgaaat gcgtccagtc aaatcgactg aggcgcatca cacaagtaat tttgctgaac
     6961 tagtatgttc caattcatta agaggaaaca gtttggctaa tgcagtaggg ttattgaaag
     7021 aagaaatgcg ccgattgaat tcggtagtaa tcacttcggc agatgaaaca gcagtgccag
     7081 ctggtggtgc cttagctgtt gaccgcgatt ctttttctga aacaattacc cgaaaagtca
     7141 aagagcatcc attagtaaca gtgataaacg aagaaattac tgaaataccg gaaggaatca
     7201 ccgtagtagc aacaggacct ttgacttctc atccattagc agaaagcatc aaagcattca
     7261 atggttcaga tggtttttat ttctatgatg cagctgcacc aatcgtagac aaagctacga
     7321 tcgatatgga taaagtatat ttgaagtccc gctatgacaa aggagaagca gcttacttga
     7381 actgtccgat gacagaagag gaattcaaag ctttccatga ggcactaatc aatgccgaag
     7441 tggcagaact taaatctttt gagaaagaaa aatattttga aggatgtatg ccaattgaag
     7501 taatggcaca gcgaggattc aaaacgatgc ttttcggacc tatgaaacca gttggattgg
     7561 aagatcctaa aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa gacaatgctg
     7621 cagcttcttt atacaacatc gtgggctttc agactcactt gaaatggggc gagcaaaaac
     7681 gtgttttccg gatgattcct gggttagaaa atgctgaatt cgttcgctat ggtgtgatgc
     7741 accgaaacag ctttatgaat tcgccagaat tgctgaaacc gacttaccaa tcaacgaaac
     7801 gcgaagatct gttctttgca ggtcagatga ctggtgtaga aggctatgtt gaaagtgcag
     7861 cgagcggact acttgctgga atcaacgccg cacgcttagc aaaagaaatg gaaccagtcg
     7921 agtttccaca agaaacagct attggaagta tggcttacta cattactcat gcggaaggaa
     7981 aacatttcca accgatgaat gccaactatg gtctgtttcc tgaactgcca gagcgcattc
     8041 gagacaaaaa agcaagatat gaagcaattg cacaacgagc tttaaaagct aatgaggcaa
     8101 taaaaacaga attagaaaaa actgcaatcc caaatgagta aggaaactcc atgaagaggg
     8161 tgagacggat agtctcgctc ttttt
//
LOCUS       DAHUWM010000122         8094 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHUWM010000122 DAHUWM010000000
VERSION     DAHUWM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.123"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(538..2169)
                     /locus_tag="NK788_001938"
     CDS             complement(538..2169)
                     /locus_tag="NK788_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCH6770264.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2183..3856)
                     /locus_tag="NK788_001939"
     CDS             complement(2183..3856)
                     /locus_tag="NK788_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCH6770265.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3869..4186)
                     /locus_tag="NK788_001940"
     CDS             complement(3869..4186)
                     /locus_tag="NK788_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCH6770266.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4211..5611)
                     /gene="lacG"
                     /locus_tag="NK788_001941"
     CDS             complement(4211..5611)
                     /gene="lacG"
                     /locus_tag="NK788_001941"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCH6770267.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5848..6633
                     /locus_tag="NK788_001942"
     CDS             5848..6633
                     /locus_tag="NK788_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6770268.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6636..7328
                     /locus_tag="NK788_001943"
     CDS             6636..7328
                     /locus_tag="NK788_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6770269.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7602..7973)
                     /locus_tag="NK788_001944"
     CDS             complement(7602..7973)
                     /locus_tag="NK788_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCH6770270.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2477 a         1792 c         1398 g         2427 t
ORIGIN      
        1 tcgcattctg ttttatacac tttacgttga aaacagagtg ggaataaaga gccaacataa
       61 tatggagggg gaatccctat aaatcaacat tcttcaatag ctaaaatgct tgattttcat
      121 taaaaataag atggttccct accaaaagag actataatat aagttaattt gtttcattct
      181 tatcataaaa agaagttctc gaaaaacgaa ctgttttccg agaacttttt tctatccatc
      241 taagtgctca aatcatattc acgacggaat tctaaggtta ccctttctta aacaaacttt
      301 atgttatctg cacaactttt tgcaattttt tcattgcttc ttacgaaatc gttgaactct
      361 ctttagcata atacaaagtg tagatgaagt ctggtgcttt actcgttttg gtaaaaatga
      421 ctggaaatcg tttctggtta tttttggcaa aggcgaggtg caggttcgat tcctgccttc
      481 tacgctaacg atgtggctga agggaacctc cgatcttgaa tcgaaacgat ccgcagtcta
      541 ttgtttcacc aatgaaacaa aatcaacgtt cgtccaagca tttcctgaag ccttgctata
      601 aaccccaatt tcacaaacac ctgttgtaat tggaatgtct ttgattgtaa tttttgtcca
      661 ttcacttgtt ttaacaaccg cagtatttag ttccgctcca ccataatttt tgatatataa
      721 ctgattagta tcaaactctc ccccagacat tacataggca ctcaatgtgt aagttccttt
      781 gcttatgcca ctaacatttt gataagctga tgcctcgtat gcttgatcct gccaaaaagt
      841 tagtttgtag tccccaaaaa aagtgcccgc ttccgttttg atcgtaccct tagcggttcc
      901 ttgttgaaac caggtttgcc aattggaagg actggtcgtt ggtccatcgc tttcgaaact
      961 tgcattttta actagattta tttgcgcccc tgcttgttta aacgcacgca agcttggcaa
     1021 ggcgtttcca gcaaaatcaa acatcgcttg gttgtcccat tcgtttccca tagtggattg
     1081 aacactgaga taattcatac ctgcttgtgt tgcccaactc acatttccgt tgtaccaaag
     1141 aggttcccaa tagaagaagc ccgccccgcg attattcggt actgcctgaa tccgatcggc
     1201 aagatccagc aaatactctg cttgtcctgt cggtgttggt tcatatcccc ccgcagctgc
     1261 ttcggcttca ccaaaagcat ttggcatagt atcggcattc gctgttgtat gcccataagc
     1321 tgtttcaact acgatcactt ctttaccata ccgttggctg atcgcattca tattggtttg
     1381 aagttcattc attgaaccgt gccaatacgg ataatacgac actccaatta aatcaaatga
     1441 cacacctcga ctcgtgattt catcaaacca ccaagtaaat gctcctgtat cgccaccatc
     1501 agctaagtgg agcattactg gagttgtttt aggttgagtg tctcgaacag cttggatacc
     1561 agacttcaaa aaagcagcca atcgatcaaa ttctcccccg ccttctcccc aagacttgcc
     1621 ataaggccag agcattccgc tattcagttc attgccgatc tgaaccatat caggataaac
     1681 gtccagctgc ttcatttccg acagaacgtt ctttgtatag tcgtacaatg ccgtattcat
     1741 ttcttcaaag gtcagacctt gccatgcttt aggaagattc tgtttcccag gatcaaccca
     1801 aaaatcactg tagtgaaaat ccagcagcac cttaagtccc agattttttg ctcgttttgc
     1861 aaggtcaatc gtcgtttcca gatcatttgt ccctgctcca taactttctc cattactatt
     1921 ataaggatcc tgccacaaac gcagtcgaac atagttgaca ccattatttt ttaaaatctc
     1981 taaggcatct tttgaaatat cgtcttcata atattttgca ccagattttt ccatatctga
     2041 caggatcgaa atatctgctc cgcgaataaa ttcgctggca gatgcttcaa cagtttttga
     2101 aacagccccc aaagccccta acacagctgc aaataaaaca gctttacgca accaaattcc
     2161 cattttcatt atggctcctc ctttattgaa attggttcag aataaactga acagcttttt
     2221 caggatctct cgttaatccg atatattctg ctccttttgt cgcaaccagc ttgattccta
     2281 agcgatcggt gtcttctttg atatcatcat agtaagaatt gacttgcggt gctaagacca
     2341 ccatatcaaa atcctgcagc atttcgtaat gagaaccata agcacccgcc attgcactga
     2401 tcggtgcttt cgattcagat gcaccttttt ttaaagcatt agctaacatc gcacttgtcc
     2461 ctgcgccagc acaaagtacc aacacattga tttctttatt tgcatcgctg ataacttgac
     2521 tcgctgccac aggtacttcc tcacgtacat cggcagaagc accttcctcg gttgcgatca
     2581 cctctgcttt ttgtgcttct ttctctacta agacagcatc gtatgctttg caaaatggta
     2641 gatagatcag gaagtcaacg acgattagaa ccgctgctag aacaaaagct aagataccca
     2701 tgcccgttcc tagaaccaaa ccgatcggtg ctggagttgc ccaaggcaaa acgtacataa
     2761 agctgttcat gcccaataca tcgacaaaaa acttgaacag gcaaacgttt acaacaggtg
     2821 ttaacaaaaa tggaataaaa aaataagggt tcaaaatcat gggtgccgca aatagcaatg
     2881 gttcgttgac agcgaaactt actggaataa aggaagcttt cccaactgcc ttcaactgct
     2941 tagacttagc aaagagtagg aagataaaag gaacaaccaa tgttgcccca gttcctccca
     3001 tagttcctac aaaattccct aaacccaccg tcagtacttt agaagcttgt tcaccggaat
     3061 tgaacaattg cagattggtt tccacattgg catagataat tgccgcaatt gccggctcca
     3121 cgatcgacgg tccatgaaca ccgacaaacc aaaacagcgc catcgctccc cagataatag
     3181 cgattcctaa gtacccgtca gctgctgtaa atagcggctg cagcaccgta atgattgctt
     3241 ctgcaaatga gatttggaag atttgtcgaa tgcctaaatc aattaaaacg gccgcaaata
     3301 ctgaaaaaga gaaaggaaaa atatctcgga aggtttgcga aatcgttcca ggaacttctt
     3361 taggcatttt aatggtaata tctttcagca cacaaaactt atataaatta actgttaaaa
     3421 atgcagcgac aaaggaagcc agtagtccct ttgtccccat atattccgtt gaaaatccac
     3481 cttcaatcgg agagacgctt aacaacagga aacttaccac agaggccagc atcacagagg
     3541 tggtgttgat tactttgttt ttcggtaagc gacgattcat cgaatcagcc aagcagcgag
     3601 cagtggttgc tgaaacgagt aaccctacaa cacccatcga ataattatag actttccata
     3661 gccatgtgga gacagattca ggccattggt aaccaaaaat ctcaggaacc gcagcaatca
     3721 aaatgaaaat gcttgaaaat aaaatagctg gcatcgctgt taaaaatccg tcacgaacgg
     3781 ctcctaaata actattacgt gagatttttt caaaaaatgg tttccctttt tcgatttgtt
     3841 taatgattcc atccatagcg ttcttctcct atccttgatt ctgtctgtac agtacaataa
     3901 agtcttgaat cagatctctc agcaataaag tggtcattaa atgatcctgc ccatggataa
     3961 aaatgaatcc catctccatc tctttcccag atgcttcttc cgctaaaatt tttgtttgcg
     4021 aattatgtgc aagggttaaa ttttcgtcgg cttctttcaa cgccgaatca acatgttcaa
     4081 agtttccttg tcttacttct ttcaataatt tcaataatgt actgcgagcg tcgcctgaat
     4141 atgcaacgat ctcaaaacca agcatttgta attcctcttt attcatcttt tcttcctcca
     4201 ttatcactta ttaaataatc gttttggttt cactgaccaa cttgtaccag taagctgatt
     4261 cttttgggta acgcttctgg gtttcaaaat caacataaaa caagccatac cttttgttat
     4321 acccatttgt ccatgagaaa agatccataa gcgaccacaa aaaatacccc ttcacattta
     4381 ccccggctgt tatcgccttg cttaaggatt ccaaatagac tctcaaatag tcgattctcg
     4441 gctgatccat aataatgcca tcttcaaact gatctttata ccccatcccg ttctcagtga
     4501 tatagatttt gttgtaatgt gggtaatcgc ttttaattcg cagcaacaag tcatatagac
     4561 cttctggata aatcagccag tcccaatcag ttcgaggaat tccttcttta taaatacgtt
     4621 ctccgattcc cttcacttta taaacagagg tccctttttc acccgtaccg ttatgatgga
     4681 ttgcattttc tccatcgtaa gctttgacaa aatggcattg gtagtgattg atccctaagt
     4741 agtcattacg cgtggatgct tttttcaatt caacgaaatc ttcttcagga aaatgatagc
     4801 tggcctgatt tgcttcacat atctcatcaa gagcagtcaa ggtttcagtt gaataatagc
     4861 ctaaataggt agcatctaat aagaaacgga tcgacaatgc atcgtctaaa aaggcagcat
     4921 gcttatcttc cggcgcgtct gttgccgcat atttcgtctc taatgagtga actacaccaa
     4981 tttcacccgg aagttcgttt tctttgaaat agttcactac acgggcatga gcaaccatca
     5041 tattatgaag acaggcgacg atctttgtaa aatcatattt gattcctggt gggaaaacac
     5101 ctaacaaata ttgattggtt gcaactggat agatttcatt aaaggtactc cacaccttga
     5161 cttctttgaa ttcatgaaaa caaaaaatgg cataggaaac aaatgcttct attgtttctc
     5221 gattcaaaaa atctccatgg tcaaacaaac gtttaggggt atcaaaatga tgcaacgtca
     5281 caaacggcgt tacgtttctt ttcttacatt cttcgaacac acgatgataa aaagcgattc
     5341 cttctggatt tggtttccca gctccatcag gaaaaatgcg gctccaagca atggataatc
     5401 ttagaccatt tacaccaaaa cgttcacaca attcaatgtc ctttgcatat tgatgataaa
     5461 aatcacttgc cggatcggga ctgaatcgcc cttgttcctc taaaaagtca tcccaagcaa
     5521 cagcgccttt gccaccttcc ttagtggctc cttcaacctg ataggctgct gtggctccgc
     5581 caaaaataaa atcttctggt aatttaagca tgtacatctc tcctcaaatc tataatcagt
     5641 tttcgctctt tctaactgat tgcattgtaa acgcatccaa aacatttgtc aacaaaaaca
     5701 aacacaaata atcataaaaa ccagaaaaca gcaataaaat ctattgtaac aaacataaaa
     5761 accaccctaa atgtttactt ttgttcgaat tagattgact gttgtccgtt tatatgatag
     5821 attatcctta aattagaggt gaagacaatg ttgaaaaaag aaagattgct tactatcgta
     5881 gaaatggtca ataaaaaagg aatccttacc gttaatgaaa tcatcaatca attagatgtt
     5941 tctgatatga ccgttcgcag agatttagat gaattagaaa aatcgggcaa gctgctgcgt
     6001 gtccatggag gggctcaaag tctttcctac actttagatc aagaactttc tcacactgaa
     6061 aaagccactc tgcagattca agaaaaaaaa gaaattgctg ccacagcagc ctccttgatt
     6121 cacgagggag aaacgatttt cttaggacca ggtacaacaa tcgaactatt ggcgacgcat
     6181 ttactgaaca agaaaattcg gatcattaca aataattatc cagtttttga tattcttgct
     6241 caagcagatg gaccagaaat tctactgatt ggcggtgatt tccggaaaaa taccggcgca
     6301 tttgtcggac cgatcaccaa cgacaattta agaagactaa agtttacaaa agcatttatc
     6361 agttcaaatg gcgttcacaa cgaagcgatc tctacttaca gtaccgaaga aggagaagca
     6421 cagcagatcg ctctaaataa ttcccgtaag aaatatctac tcgttgataa taaaaaattc
     6481 aatcgagatg atttttatgt gttttataat ttgcatgatt tcgatctgct cattacggat
     6541 cgccagatct cgcgggaggt tcaagagcac tatgcacaat atgtcgatct gcaaattgct
     6601 gattcaaccg aaaataccac tataggagcg taactatgaa agggattata tttgatttca
     6661 acggaacaat gtttttggac tctcatctgc atgaagctgc ttggctgcat atgatacatg
     6721 atcatacaaa agatggttta tcagacgagg atattttaaa aaatattcat ggtcggacga
     6781 atacggagat tttgaaacat ttcatttcag agcatttgac cgcctctgag atcgccgtat
     6841 tgtcagaaga aaaagaaagc tattatcgta tgctttgctt gcaaaatgaa aacgagcttc
     6901 agttgacaaa agggcttgaa gcgactcttg accagctgac tgaccaacag attccattaa
     6961 cgattgccac ggctactgtc aaagagaatg ttgcttttta ttttgatatt ttcgatttag
     7021 cccgctggtt tgactgggac aagattgtct atgacgacgg ctctttccct gggaaaccgc
     7081 aaccggatat ttttcttaaa gcggcagaca aactgagctt agctccggct gattgtcttg
     7141 ttatcgaaga tgcttactca ggattacttg ctgccaaacg tgcggggatc ggaacaatta
     7201 ttgcaattga tccattcggt aaaaacaaaa cagtatttga acaggcacag ctaaataaag
     7261 atggaaccat caaagatttt actggttttt ggacagagca cctgtctgcc acatccgtta
     7321 attcttaaac gattcaacta actaccaaag aaaagcaact gaaatgcaca cctcctttta
     7381 taaaaaactg ccagtgaaac gacatgaaag atacactgct atatccttag tgaaatatag
     7441 cagtgtatct ttgacttttt cattctttta accatatgac gcgttcatct ctatctataa
     7501 ttaacattaa aaatagtgtt ttcacattat ttctcaaacc aatcttaaaa atcatcagga
     7561 gtactttttt gatatacttt tattaatgct gggcgtaatg gttaaaagat ttccactagt
     7621 tcctctccac caagctgagt tgaaaaatgc ttatctgtaa acaacataga caaagaggaa
     7681 ataaacgttt ccgtatcttt ctctgtccca aggatattaa ttaattttcc tactaggtaa
     7741 gtatcattaa acttaagatc tgccttcaca tcaaaagttt tgataaaagc ttttatcatg
     7801 cgtacaattt tgtctttatc gccataattg atttcaccac tcatatcaat atagtcacta
     7861 atccctgaag aaataatggc aactgcttct tctttcgcct tatcagataa ttcatttaat
     7921 gactgggatt gattgatgac ttcttgagtt ttatctgtta cgtaatctct cattatccta
     7981 tcggcatact ccgcaaagta atcttgaaaa aattcttcta cagggtaacc aacttcgttt
     8041 tcccaaccag gagaaatttc attatcagta cttattaaat ctattaaaaa ccaa
//
LOCUS       DAHUWM010000123         8035 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHUWM010000123 DAHUWM010000000
VERSION     DAHUWM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.125"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="NK788_001945"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="NK788_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCH6770271.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1229..2332
                     /locus_tag="NK788_001946"
     CDS             1229..2332
                     /locus_tag="NK788_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6770272.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="NK788_001947"
     CDS             complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="NK788_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCH6770273.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3822..6269)
                     /locus_tag="NK788_001948"
     CDS             complement(3822..6269)
                     /locus_tag="NK788_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCH6770274.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6458..7894)
                     /locus_tag="NK788_001949"
     CDS             complement(6458..7894)
                     /locus_tag="NK788_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6770275.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2234 a         1739 c         1374 g         2688 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaaca aaaattcatt attgtcgttt tcaatgtttt tgtacttgtt cgtttttagg
     1081 atacttattg atgatttcaa ggtgttcatg ccaccttatt catcacttat cctgtcttag
     1141 gttcacctct actccatcta cagccgcagt cagatcgatc actctttaaa attttaaccc
     1201 gtttcaacga agaaagaagg aaatgaatat gagtcaaagt gttttcttac caaattatac
     1261 cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc aaagaatatg ggacaaaagt
     1321 agtgctgatt ggagggaaga ctgcactttc aaaggcagaa aacaaaataa aagtagcttt
     1381 gacacaggaa atagaggttg tcgccagtct ctggtacggg ggtaattcaa cttatggaaa
     1441 tattgaacag ttgatccagc atgaaagtgt ccaaaaagca gacgtcctgt ttgcagtcgg
     1501 tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa aaactagcta aaccagtctt
     1561 cacctttccc actattggtt cgaactgttc gcccgtcact gcggtatgtg tcatctataa
     1621 cgaagaaggc gtaatggacg gactcttctt tccttcaaaa ccacctgtac acaccttcat
     1681 tgatacagca attattgccg aagcacctga taaatactta tgggcaggta ttggcgatgc
     1741 tttatccaaa gaagtcgaat ctactttttc tgctcgtggc gatgatctag gctacacgga
     1801 tagattagga atccaaatcg gaaaaatgtg cattgatcgt ctcttgactt atggtacgca
     1861 agcattgaaa gatagtgccg acgatcgtcc ctcatatgca attgaacaag tcgtttcgga
     1921 tatcattgtt actaccggat tggtttctgt tttagttaac aataactata attctgctat
     1981 ggcacactca ttctattacg gtacgactgt tctagaaaaa atggagggat accttcacgg
     2041 agcatctgtc agttatggtg tactgacctt attgatgatg gatcagcagt tcgatttgtt
     2101 tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca gtatcgttaa ccgacatgaa
     2161 tctcaatgtc acgaccgatt tagaagcaat cttagataaa gccatggcaa caaatgatat
     2221 taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa caggccattc ttgacgtaga
     2281 aacttatcat gctacacaag aaagccagca aggatataag aaaatcagtt aaataattgc
     2341 taaaaccccc atcaagcagg ctgtcatcaa attgaggaca tttctttgct tgatgagggt
     2401 ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa tcttttctct tatttaagtt
     2461 gaacagttcc ttaattaaga aaactgctcc ttttatggta attgaaaaaa cgagtaggat
     2521 acgattattt ggcaactgtt tttttcattt gcttgcttcg caattgtccg caagctgcat
     2581 caatatctgt accatgttct ttacgtatca cacagtttac gccgtttttc ttcaatacat
     2641 cataaaatct caatacatca gcttttggac tgcgagaata ttgatcatgt tcactgactg
     2701 gattatatgg aatcaagttg acatatgcta attttttctt atcttttaat agatctgcta
     2761 attgttgcgc atgctctggt ctatcattta cttgattcaa catgatatat tcaaacgtga
     2821 ttcgacggtt tgttttctct aaatactcat ccacagcttc catcaatttc tcaattggga
     2881 aacttcggtt gatccgcatg atcgatgttc gcacctcatt attcggcgcg tgcaaggaaa
     2941 tagccagatt gacttgcaag ccgttattag cgaattcctt gattttgtgt gctaatccac
     3001 tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc tttggcatca ttgatgatat
     3061 gcaaaaagtc cattacattg tcgtaattat caaatggctc accgatcccc attacaacga
     3121 catgtgaaac gcgttcgcca agattgcgct catcaaaata atgttggacc atcatgatct
     3181 gtgcaacaat ttctccagca gtcagatcac ggttcttttt gagcagcccg cttgcacaaa
     3241 acgtacagcc gatattacat cctacttgtg tcgtcacaca aactgacaag ccatattctt
     3301 gtctcatcag tacagtctca atcatgtttt tatcagggag ttcaaacaaa tattttactg
     3361 taccatccga agcttcttgg acgaccatct gttttagagg attgatgata aaatgctccg
     3421 ataatttttc gatcaattgt ttcgatagat tcgtcatttc tgtgaaatta cttacacgtt
     3481 tttgatacaa ccattcccaa acttgggcag ctcggaattt tttctcgccg ttttctataa
     3541 accaattgat taattcttca ttcgtcaagc cataaatgga ctgtttttcc aaagtatttt
     3601 ccctcttttt ctcaaagttt cgctacatac tatacatgat tcatttcctt aatgcaatac
     3661 ttcacaaata aatcgaatag aactttaatc tcacaaaaac taaaaagaca agacatcata
     3721 ccgtattaga aaagatggaa aaaaaccatt ttttcttttg gtatcatcat gccttgtctt
     3781 tttattggtt attcgttttt caacccttca tcacacaagt tttacatgtc attatcgtcc
     3841 ggcaacgttt ctgatgtttc ttcctgctca atgaatactt tccgaggctt gcttccttct
     3901 gacgggccaa caactccatg agcttccagt tcatctacta aacgtgctgc tcggttgtaa
     3961 ccgatccgaa atcttctttg gagaagggaa atacttgccg tttgcatttc aacgacaagt
     4021 gcttttgctt cttcaaacag ctcatcttgt ggctgttctg gagcacctgt cgtttcaact
     4081 tcatctgtcg gcatcatttt ctcttcataa tgagcttctt gctgatctgt aacaaattga
     4141 acgacccgtt ctacttcgtg gtcagaaatg aatgctcctt gcacacggat cggtttattc
     4201 tcacccattg gcaagaatag catatctcct cgccctaaga gcttttctgc cccattagaa
     4261 tcaatgatcg tccgagaatc tgtaccgctt gataccgcaa atgccattct ggaaggtaca
     4321 tttgctttga tgattcctgt gataacatcc acacttggac gttgagtagc taggatcata
     4381 tgaatccccg ctgcccgagc catttgcgct aaacgaataa tcgcatcttc aacttcattg
     4441 ctggctacca tcatcaaatc agctaattca tctacgatca ctacgatgaa tggtaaggtt
     4501 ggatgtttcg ttccgttttc caagtttttc tgttgaacaa attcattgta accagaaata
     4561 tttcttactc cggttgccgc gaatttttca tatcgttctt ccatttcttg tactactttt
     4621 tgtaaggctt gcgctgcttt tctagggttc gtcacgaccg gcgtcaacag atgaggtatg
     4681 ccgttgtaca tattcaattc aaccattttc ggatcgatca tcatcagttt tacttcatgg
     4741 ggcttagcac gcattaatat acttgtgata atcccattaa ttgctactga cttcccacta
     4801 ccagtcgaac cagccacgag taagtgaggc atcttcgaaa gatcagccgt ttgtacccgt
     4861 ccagaaacat cacgtcctaa aggtacttct agtaatttat ctgggtggtt tggttgtgct
     4921 tcgatgattt ctctaaagga aaccatactg atctttccat ttggtacttc aataccaatc
     4981 agtgatttac ctggaatcgg tgcttccatc cggacatctt tcgccgctaa agcaagcgcg
     5041 atatcatctg ttaagttcac aattttactt acttttaccc ctacagccgg ctggacttcg
     5101 aattttgtga cggatggtcc taagctggct ttgacgacct tggcatcgac accgaaactt
     5161 tggaaagttt gttctaatac gccgatattt ttttcaattt ttttatactc atcactttga
     5221 tccacagttg gtatagaatc aagcaaatca actgtcggta attcatagtc ttgattttcc
     5281 tgttctgctt caatctcaaa ttctaatgag gtaccgtcat cctctggcaa ctcatctgaa
     5341 aaaccttctg ctgtattggt ctgttttggt gtatctggta ctggtttctc tagattcgca
     5401 ggatggatat tttcttgaaa actgtcgatt ggaacaaaac ttagctgctc cggctctgta
     5461 ttttctggtt cctgccattc atcagcagta ttttcccttt gctggctcac cgcttttttc
     5521 ttctcatatt ctgcagcttc tttttctgca gcttcttttg cagctaagcg ccttgcttct
     5581 ttcgcttctg cgcgctgttt cattctctct tcctttgccg cttgctttcg agcttgcttg
     5641 gcaggatcac cttccaataa ttcctgtact cgttctccaa ttgattggat accatttaac
     5701 agctgatagc cttccagatt actgaacagg aatgctcctc ctgccagtaa tagcactgct
     5761 gctaaataac ttccaggctg tgcaatcaag aagtaagtaa aatgataaag gattgctcca
     5821 atcatcccac caccaacgtt attgtctact tgactttgtt ttatatcact ggctaacaaa
     5881 tcccatgtcg tccccatgat atttggttcc ccagtatgga gtttgccaaa taaatgggca
     5941 tgtaacaata ataagatacc gaaataaaag agtatgccgc caaaccagcg tcggttttta
     6001 ccaattgaaa actctgtatt cttaatgaca atccaaaatc ctaatacggc taataaaaga
     6061 cagaggattt ggtaagtatt tcctgcaatg atccgaaagc cgtttgcaat caatattcct
     6121 aaaaaaccta attttaaaaa gccgaagaca gcaaataaaa tcaaggcaaa cccaacacca
     6181 atcgtcagca tttgttcctg ctgctgtttt tgctttttcg tttttttcgt ctttttcctt
     6241 tttttagtag gccgtttctt ttgtgccatt tattatccct catttcatca agtttcatta
     6301 tacaaaaaaa agtaagggaa ctaaagtttt gtttgaaaga actgacgctt tctttcattt
     6361 ttcagcttat tttacagttt ttcttaattt ctaaatgatt ttatcaagaa aaggcacggc
     6421 catatccagc cgtaccttct tgttttaagt atgtgaacta aaggttctct ccattttctt
     6481 caatcagttt ttggtaccag taataactgt ctttcttata tcgttccaaa gatccttctt
     6541 gtgtttcgtc ttgatcaaca tatacaaatc catatcgctt ttgataaccg ttcagccagc
     6601 taagcaaatc tgtataagac caggtgcaat agcctaggac ttctgctcca tccgaaattg
     6661 cttctttgat ggctttgacg tggctttgta agtaattgat tcgatagtcg tcatggattt
     6721 gtttatcttc tgtcaacttg tcatactctc ctaagccatt ttccgtaatc agtaccggca
     6781 tgcggtaacg gcttgtgata cgacgcagtc cgatccttaa tccttctggg tcaatctccc
     6841 agtcccaatt tgttcgttcg acaaatggat tctcaacttt tttatacatt ccaggaactc
     6901 cagtttcttc tgatgaacct ttttttcctg tcgtattcat ttttccagca ccaactccgt
     6961 ttaatggatt gaatgcattc gtcgccgttt gatagtaatt gatcccaaga aaatctggtt
     7021 tagcagattc tagcaattct gcatcgccag cttcaatcgt tggtgcccat cctttttctt
     7081 ctaagaacct catggcagca atcggatatt ttccccacat atagacatcc atccaataat
     7141 tggccatcag gtcttctgca ttttcagcag ccagaacatt ttcaggatta ctgtcaattg
     7201 gataatttgg cgtatacgca aagcttggtc cgatttttcc aggcatttct aactcgtgaa
     7261 atttattgat gacagatgca tttgctaaat tagcaatatg gttaacttca tacattcgtt
     7321 tcgggtctgt cacacctggc ggatgtgctg ctaacaaata acctaatgaa gtgaagatat
     7381 tttgttcatt caaactaatc cagtaatgga ctttcccgcg gaatgcctca aataatgttt
     7441 ctgcataatg agtgaaatct tcaatgatct gacgtgattc ccaaccatta tattcatctt
     7501 gcaatgcttg tggaagatcc caatgatata aagtcaacat aggttcaata tcgttttcaa
     7561 tcaattcgtc gatcaatctc tcataaaaag cgagcccagc ttgattgact tctcctcgtc
     7621 cattaggaaa aatccgtgtc caagctacag agaagcggta agctttcaga ccttgttctt
     7681 tcattaaagc aatatcttct ttataacgat gatagtggtc tactgccaaa tcaccgttcg
     7741 ttcctttaaa tgtctttcca ggtatccgta caaatcgatc ccagactgat tctcctttcc
     7801 catcttcttg ccaagctcct tctacttgat acgctgcaga agcagagccc cacagaaaat
     7861 cttttggaaa tgaggtaagt tctttgtgtt ccattcatct cttcctttcc tataaccttc
     7921 aatcgttcct cttttagaat aacacgaaac cccttccaca acaaaataaa attatgtaaa
     7981 ggaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg aaaat
//
LOCUS       DAHUWM010000124         7954 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHUWM010000124 DAHUWM010000000
VERSION     DAHUWM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.126"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..286
                     /locus_tag="NK788_001950"
     CDS             <1..286
                     /locus_tag="NK788_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770276.1"
                     /translation="EKLYVVKDIALDLNYTLAKMNSVIQQAESFCERYPEYKLSFLSE
                     NKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSLYKGTYQSLENLSQKII"
     gene            289..1446
                     /locus_tag="NK788_001951"
     CDS             289..1446
                     /locus_tag="NK788_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770277.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1515..2855)
                     /locus_tag="NK788_001952"
     CDS             complement(1515..2855)
                     /locus_tag="NK788_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6770278.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(3014..3454)
                     /locus_tag="NK788_001953"
     CDS             complement(3014..3454)
                     /locus_tag="NK788_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCH6770279.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3614..4120
                     /locus_tag="NK788_001954"
     CDS             3614..4120
                     /locus_tag="NK788_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6770280.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4288..6201
                     /locus_tag="NK788_001955"
     CDS             4288..6201
                     /locus_tag="NK788_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCH6770281.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            6206..7207
                     /locus_tag="NK788_001956"
     CDS             6206..7207
                     /locus_tag="NK788_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6770282.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7409..7615)
                     /locus_tag="NK788_001957"
     CDS             complement(7409..7615)
                     /locus_tag="NK788_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCH6770283.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2791 a         1275 c         1602 g         2286 t
ORIGIN      
        1 cgagaaatta tatgtggtga aggatatcgc tttagatcta aactacaccc tggctaagat
       61 gaattcggtg attcaacaag cagaatcgtt ttgcgaaagg tatccagaat ataaattatc
      121 ttttctttca gaaaacaaga tgataaaagt cgagttttct tctcagtttt tactgagtaa
      181 agtttattct atacttttag agggaacgat tggttatatt ttactggatt cactttacaa
      241 aggtacttat caatccttag aaaatcttag tcaaaaaatt atataagttt gcgtacgatt
      301 caaagaaaat taagagaact taatactatt ttgaaaaact atcatttatc ttgcagcttg
      361 aaaaaaggta atccgataga aggagaagaa taccgtatcc gatacttttt tcacttgatg
      421 tattggcagt cattcgacga aaatcattct gatatattat caagtataaa aaataaacgt
      481 caactattag aaaaatttgc gaaacatgcg ccatatacgc gtagtataga tagaaagaaa
      541 tggatcagcc tactaagaat ttcgttgcaa cgaatgaaac aaggccactc gattacgtat
      601 gtcccagaag agatgaacaa atttgtgcat ccattgataa caaaagaaga gtttacgcat
      661 tacatactag agcctttttt tgaagcaaac ttctggtcga tgaatcacat cactccaaca
      721 gagcaagctt acctttattt tatgtttggt gtaatgaaca cctatttgga agaagatttg
      781 ttggatactc cagataaagt acaagaaagt tggggctgga ttgaatcgtc tattgtagaa
      841 aattttgaaa aaaacttcaa gatttgtttt acaacttcag aaaaaaatta tctatatgtc
      901 aatttggcaa tgatccattt gtattcaagg gtttttggga caaaaaagaa ggtggatgct
      961 tttggaaaat cagcagaaga cagagatttt cagcatatat ttccagtgat gtatccaatc
     1021 atgcaacagt tttttagtgg ggtagctcgc agaattccag agcttaatac ttattatgaa
     1081 aagaacagac ggttgatttt tcaatactgt atgttagtca gggatatttt tataaaacac
     1141 gaacaacctg tcatattcta tattcaatca aagtatggta aaacacagga attatgggca
     1201 aagaaaagga tattatctat gactgaacgc ccaataaaat atagtgcatc cgctgatcag
     1261 ttacctgata ttgtcattag cgattatcct atcgacaaaa aggcatatga tcaaacgaat
     1321 acacagatat tttactggaa tggacaacct acaaaaaatg attgggcgcg tctgaaagct
     1381 gaaatcataa aaatcaggaa tgagaaaaat agatccatat tggaaaaatt ggatttcgtt
     1441 aattaagaaa agcggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg
     1501 agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc
     1561 agttgggtag tgttcttagt tttcttgaaa tattttcctc gatataaaaa gattcgttct
     1621 cggtagtttt gaaagtttct aaagccatag gcaattcgtt ttagtacttt gatatggttg
     1681 ttcaaacatt ctatagggcc gttggaatag ggaactcgca acgctcgttt gatttctgtt
     1741 cgatatttct tgaaggtcgt gaacactggt tgatagtgct ctggcaactc cttgtagtct
     1801 tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgatagcgga aagaaaaacc
     1861 tggtagactt cgtagcctcg ctttaaggag tcatcataat ataatagccg atcgactatc
     1921 tccgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg accattcaat
     1981 tcccacgcat tcttttgaag tagtctccag tatttcttta agtgcttcga ccgacgatcc
     2041 ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaggcccg accgatgtgt
     2101 tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat
     2161 gtgcgatagg gtgtatagaa atctgaaacc actagacgta cttgttcacg gacttttcta
     2221 gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc
     2281 agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac gtttttcacc
     2341 gatttgaatt catcgaaaca caccacttct ggtaacggct ggttcaaaga aacggtttta
     2401 ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt catacgtgca
     2461 atcaaagaca tagaaatagg ctcacttaat agctcaagaa tggcttgttt gactctgcgg
     2521 gcaatggagc agcgacgatc gctaactgaa tcttctgcta aaaaagtctt tccacagtgt
     2581 ttacatttga agcgctgttt gttaatccgc acaatggtct tgtagttact gacatcattc
     2641 aataagatgg atactttttt ccagccccat ttaatgatcg tttgcttatt ttgatagtgg
     2701 caatggaagc attcctctgg ttgataggtc aatgtgccat caaaaacaaa acatgtttcg
     2761 tcattaatgc gctctttgcg aaaggcatct tcatgaaagg taagatttag gtctagaata
     2821 tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt
     2881 tcgtcgactt tattctaaag gatttcaagg tgtctgtttg cgtttattag aaaaaagaaa
     2941 aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatccaga aaagcagatt
     3001 gtttatgcca aaattaagac attgcaaaaa gattattttc tttaaaatcg acaagatgac
     3061 cagaaagcaa gagttcgttg agctcttgct ttcttttagt tttagaaagg aacatcctga
     3121 ttttatcttc gctaaatcca gtcaaaagtt ttgcagattg gtcgtagata atcggttttt
     3181 tcaataaaga aggggatgta cgaatctcaa tatatagttc actcaaactg agttcatcta
     3241 ttctgtgaac aagattttta taactaatag aatgagtaga gagcatttcg gtcacgccgt
     3301 tgtttgtcaa ttttagtaag tggataaatt catttttggt tagtggttga cgaattaagt
     3361 tctgttcgat aaacggtata tcatggacga taagccaacg ttttgcgcgt cttgaagacg
     3421 agcagccaga acctgtatac aatttaatca tcatacattc ctccaatagc taagtattta
     3481 tgaaataata acatgaagaa atagtcgaaa agtgccattt agaggtgtaa aataagaaaa
     3541 tgttgtcacg tataatggaa ctagctagat aagaaatact attaaatcaa gaaaaaagaa
     3601 agaaggcaca agtatgtcaa acacagccga cgcaatagta gtgatcgatt tacaaaaagg
     3661 ggtatgtttt gattcttcgc cgatacacga attagaaaca gtgacagcat tagtgaataa
     3721 aagaatcaaa acatacgaaa cgattgggaa accagttatt ttcgttcaac acgaagatgc
     3781 tcaattagtg ccacaaacaa aaccatggga gctattgtcg gaattatcta ttcctgagca
     3841 cagttatttt gtgagaaaaa cccatgcgaa ttcattttat aagactaatt tgagtgtttt
     3901 attaactgaa ttggactgtc attcgatcga attatgtggg gcacaagcag agtattgtat
     3961 agatacgact gtgaagtttg ctcatggatt aggataccag cttatgatgt gtagaggagc
     4021 aacgacaact tatgataatc cttttatgaa agcaaaggaa acaatcgctt tttacgagaa
     4081 aatttgggat aaacgatttc tgacattttt ggagcaataa gttgtttctt cgtttaaaaa
     4141 aagcagaatg gatactggtt tatccattct gctttattac gtaaaatgag aaaataatcc
     4201 tgttctattt ttatgggata aaccatttat tttttgtata aaaaatgttt gcgtttacat
     4261 ataattctcc tagaaacata aaaaggagtg aatagcttga gaacaaaaca agtattaaag
     4321 ggagcgttgg tcatcttatt tgctgtaagt acaagcgtcc cgcaatggac agtatatgga
     4381 gagagcattt cttccgaatc ggaatctcgg gaggtagaaa ccacagaaac tactgatgct
     4441 ggaaatgaaa cgaaatcgag tacttcacaa acagaaacaa atactgaaac aacggttgag
     4501 acatctgtta ctagcacaga tcaagaaacg accgctacca cagataacca acaaacagaa
     4561 gaagcaacag ctacaaaaat gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa
     4621 gagaatggga cagaaaatga tgatatcggt aaaggtgaaa atttcaatgg taaagaatat
     4681 ttgaattact tcggtaaaca gatgacagaa atacttaatg gaccaacaga agacatcaag
     4741 tcaaaattaa tagagttatt agtcactgag cttgctgtta atgattatgg tgtagataaa
     4801 gagattatca aacatctgat tgctacttat ctattgtacg atatcgcttt tgcaaaaaac
     4861 agtgtgttga tgtcgatgct tggaggtaca gatgatggga caacgcctac agaaaacaca
     4921 ggcttctatc caaatacaaa acaacttcat caaactgtac taaacaaaca agatggcaaa
     4981 atgatggatg tatatgcata ttatgtcgat caaggttcag ataaaacagt catgatccat
     5041 ggcggatttc gagggaattg gaataacggg atcgttacag aagaatacaa tgatttttat
     5101 aaggccgggt ataatttatt atttgttgat tccagagcga ctggtaatag cggaggcgat
     5161 tatgtgacct atggacaata tgagtcagat gacgtccttt actggataaa tcaagaagtt
     5221 cgagagcggc catctcaaaa gattttactt tatggtggtt cgatgggagc agctacgatg
     5281 atgtctgtat tagcgaaaga tattcctgtc aatgtcaaag gaattattga aaattgcgga
     5341 tttgcatcaa ttgatgaaca actacgtttt acgtacagcc agacagtagc ccccgcacta
     5401 ccggatgcca taaaaaatca gttagacatc attggggatc aagagcatga agatttattt
     5461 atgggccttt tgaaacaata ctattttgat caggagatgc atttggatac aaaagctgct
     5521 ctacctacga ttggaatgtc agattcattg cctaaattga ttatccatgg aacagcagat
     5581 gatgtagtac ctgtgtctaa cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat
     5641 cttttattag tagaaggagc agggcatgga aaggcacagg aagttgacca tgctgcctac
     5701 acgaaacatg taacggactt tttgaaagta gtgttccatg atcagataaa cgtaaagtat
     5761 gtggatgaaa acaataaaag tctactcaaa gaccaggacg aaatccagct ttacggagct
     5821 tatggagaaa attatatgac agagcaaaag acattcgaag gatacgaatt agcaaatgtt
     5881 gaaggaccga ctgaagggat ttttaatgag acgataccaa ctattatttt ctattacaaa
     5941 aaaatcccag tagctccacc aaaaaagcaa gatccgacag agaatgcaga caataaggga
     6001 aagaatgaac ctcgagagga tcaatcaagt gatgatacgc aaccgattca gatgacagtg
     6061 aaagtacaaa aaaagacaga tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa
     6121 cattcgcttc tcatttattc aggtggattt ttatctgtta gcacgattat cacatggatt
     6181 tggcgaagaa agagagggta aatgaatgaa atggttcaag cgaatagtga ttgcagtacc
     6241 agttatcatc gtagttattt tagtcactgt atttttgatt aatcaaatca caccaaagcc
     6301 gattagcttg cttgttagaa aacaatttga cacaagtgaa aaagaacaag tatatgcccg
     6361 accagatgat ttccagcaaa aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc
     6421 ttcaaagtac aaaaataata atatggatat ctacacaccg aaagtggaaa agaaaaaatt
     6481 accgattctt ttctggatgc atggaggcgc atatgtagga ggggataaaa atgattgtcg
     6541 tgattatttg gaatatctat gttcagacac gcagcaaatc atcgtaaata tagattatga
     6601 acgatcaccg gaagcaaaac atcctacacc agttatccaa ttgaacgaag ccattcaagc
     6661 agctaaaaaa gagataaaag atcaagcaga ttggtcgaat atttcgattg gtggaaattc
     6721 ggcaggtgca caaattagtg gagaatattt gttagcactt caaaatgaag aaattaaaaa
     6781 agctgatcat cttgatccga ctttggagaa caagcagatt acgaagttta tttctttgtc
     6841 tggtctgttg gatccttctg cacttactca agtaagtgat aaaatctcta gctttcttta
     6901 tgaaaaatgt ggatgggctt actttgataa taaggacttt gaacaatcag agaaggtcaa
     6961 aagtttagcc ttggtcagac atgcggatcg ttggacgcaa aacacattct taacggatgg
     7021 aaatacaaat acgtttacga aacaaatgga agaagtaact tctgtatttg agaaagcggg
     7081 ggtgaaagtc acaaaagtag attatgcgga gaaaaatgct gttttgaacc atgaatatca
     7141 gtttgatttc agcaataaac aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa
     7201 ggagtaaagt cttatgatgc aaaagaggtt gtaatctgtc acttagctcc aagtcacaag
     7261 gagcaatttc aataaacaag cttttcatgg tttatgaata ttgtaacttg tgactgagga
     7321 gttagagatg gcaaggggca ccttttattc gaaatttggg aatatcacac gattattgtc
     7381 acgatctctt ttacgtaaat tttataagtc agatttgttg ttttgatcgt ttaaaaaaag
     7441 caacaaatcc gaagcctgcg gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga
     7501 atgctggttc cactaatatt ccgttgttat tgatataagg ctgggaacaa agatgaatag
     7561 caaataaaat caatgcacta gcgaaaaaaa gcaaagaaat atggatagct ttcataagca
     7621 gcacctcctt ttttataatt gtatgataaa tagattaaga atctataaat gtgacaagtt
     7681 tgttaggatg tgaataaaat gatcatctag cgaacttgaa acaaagatgg ctaatgataa
     7741 gcaattaatg attcagcaat cagataggta caaagtttac catcatacgt ctagaccaat
     7801 aatagaaaat atgggaatat aatagaaaag atttaggata cccccaaaag aatttcccca
     7861 aaaattcgat gtatcctaaa cttttttatt ttacataaca atcatatcaa ctgtgaaata
     7921 aaattacaaa tgataaccta aaatgaggcc aaaa
//
LOCUS       DAHUWM010000125         7939 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHUWM010000125 DAHUWM010000000
VERSION     DAHUWM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.127"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..823)
                     /locus_tag="NK788_001958"
     CDS             complement(<1..823)
                     /locus_tag="NK788_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6770284.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAI"
     gene            complement(1047..2987)
                     /gene="thrS"
                     /locus_tag="NK788_001959"
     CDS             complement(1047..2987)
                     /gene="thrS"
                     /locus_tag="NK788_001959"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCH6770285.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3324..3503)
                     /locus_tag="NK788_001960"
     CDS             complement(3324..3503)
                     /locus_tag="NK788_001960"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCH6770286.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3550..5373)
                     /gene="cadA"
                     /locus_tag="NK788_001961"
     CDS             complement(3550..5373)
                     /gene="cadA"
                     /locus_tag="NK788_001961"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCH6770287.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5651..7930
                     /locus_tag="NK788_001962"
     CDS             5651..7930
                     /locus_tag="NK788_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6770288.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2320 a         1696 c         1444 g         2479 t
ORIGIN      
        1 ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta gccatataga
       61 aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt gccgcacttc
      121 gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc ttgctaagcg
      181 ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc gccagctcgg
      241 ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc actttctttt
      301 tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa atgattggct
      361 taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag gcgccaaccc
      421 catataaaaa aacagtctct gccgattcca aacactctac gactttttgg atcgtttctt
      481 cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca agtttgtttt
      541 tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg attccagtct
      601 gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg atttttttgg
      661 aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca ttagctgtca
      721 ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt tctgctgaag
      781 aataatcttc catcatactt tcaatacgac taattacgct cataagttcc tccacccttt
      841 taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt atactggata
      901 aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc acagattcta
      961 ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc tttctatcta
     1021 ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt gacttctgct
     1081 tccatagcat caataaagat attcaaatct tctacagctg tttctttact tccataacga
     1141 cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg gtaagggatc
     1201 ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc atcaacttct
     1261 acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc gccatgtgca
     1321 tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg aaaagcgcct
     1381 ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat tccgcggtgg
     1441 ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa atcaaagcgc
     1501 tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc aagagctgtt
     1561 ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa
     1621 tctagttcta attcatccat agcggctttc agcattgctt gggctttttc ccacattgcg
     1681 tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg gaaacgataa
     1741 tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg tttaaattca
     1801 tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca
     1861 cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc taattcagcg
     1921 atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat catgtgatga
     1981 gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat tggcgggaac
     2041 atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac atctcccatt
     2101 attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat ataacgttcg
     2161 atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga acctacttct
     2221 ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct ttcttttgct
     2281 tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat
     2341 acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc cactgataat
     2401 aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca caaatcgatg
     2461 aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact aatcagttct
     2521 actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag aacacggcgt
     2581 tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc cggcaaatct
     2641 tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga atcgatagct
     2701 ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc catcaaatga
     2761 gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat gatctcaata
     2821 cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac tttaccagct
     2881 aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca
     2941 gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat agtttcctcc
     3001 ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct
     3061 tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg
     3121 ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc
     3181 atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta tcgaagactc
     3241 tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac taataaatag
     3301 aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc gtatgttcct
     3361 tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt gtttttatga
     3421 acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg accttctact
     3481 aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc ttttttcaac
     3541 tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt
     3601 ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca aaacagctcc
     3661 gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac aaataaagat
     3721 gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca ctactgcttt
     3781 tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac tagcagctgt
     3841 agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg gtgcgtcatt
     3901 cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt ctaaaatggt
     3961 cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt tttctgcaat
     4021 cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca catcaagttt
     4081 tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt
     4141 tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt ctatttcttc
     4201 agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta ctgcttggat
     4261 tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt ttttcacgtt
     4321 gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag ctgcatacag
     4381 aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt ccaatatccc
     4441 tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat
     4501 cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc ctgcaactaa
     4561 agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta cctcagcaaa
     4621 tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag caatcaacgt
     4681 gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt cttttgattc
     4741 ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt cagcgatcat
     4801 ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag gtttcttggt
     4861 ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc ctttggtcac
     4921 tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat caatatttat
     4981 ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa ttcttggggt
     5041 gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat cctctagact
     5101 atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt tgacggctaa
     5161 tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg aacgtaacgt
     5221 cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc
     5281 aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc caccaataag
     5341 caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt tatgtgactt
     5401 agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt ccttgactag
     5461 cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg tttccaaaat
     5521 tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc atcagttaaa
     5581 aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg gcggaaggag
     5641 caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa cgatcgaact
     5701 aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc aggaaatgaa
     5761 tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg aagcttttta
     5821 tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat accatactct
     5881 tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc
     5941 ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta tcgcttcttt
     6001 aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg ccaatcttta
     6061 ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa tagatgtcga
     6121 tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta tggtagatac
     6181 gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat caagcaacca
     6241 tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc gggacacaac
     6301 aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg ctttgcttca
     6361 acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa acgttcttct
     6421 attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt cattaggtga
     6481 aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc cagagttttc
     6541 tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag atccgataca
     6601 agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc aaggcatcac
     6661 aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc tatcaaagga
     6721 atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc atagactttc
     6781 tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca
     6841 gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa cagatccaaa
     6901 tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca accaaatcgt
     6961 acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg tcaatataac
     7021 gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac gacataaaat
     7081 cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc gtcaaagaga
     7141 attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat atccagtgac
     7201 ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg caccatcaca
     7261 agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg gagatctaaa
     7321 tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt tcgtggaccg
     7381 tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa tcactgattt
     7441 tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa aaggagacct
     7501 tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag cagataccat
     7561 tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta aaacacaaga
     7621 agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag tccagctgat
     7681 tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct ttttagcaaa
     7741 aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt tttcttctca
     7801 gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat tgaccctttt
     7861 tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt atgagaaaaa
     7921 agtactgtaa gtaaaaaga
//
LOCUS       DAHUWM010000126         7906 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHUWM010000126 DAHUWM010000000
VERSION     DAHUWM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.128"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            264..992
                     /locus_tag="NK788_001963"
     CDS             264..992
                     /locus_tag="NK788_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6770289.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            1010..2206
                     /locus_tag="NK788_001964"
     CDS             1010..2206
                     /locus_tag="NK788_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6770290.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            2199..3194
                     /gene="lacD"
                     /locus_tag="NK788_001965"
     CDS             2199..3194
                     /gene="lacD"
                     /locus_tag="NK788_001965"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCH6770291.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            3191..4132
                     /locus_tag="NK788_001966"
     CDS             3191..4132
                     /locus_tag="NK788_001966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HCH6770292.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            4308..4787
                     /locus_tag="NK788_001967"
     CDS             4308..4787
                     /locus_tag="NK788_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770293.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            4800..5690
                     /locus_tag="NK788_001968"
     CDS             4800..5690
                     /locus_tag="NK788_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770294.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            5677..6486
                     /locus_tag="NK788_001969"
     CDS             5677..6486
                     /locus_tag="NK788_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6770295.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            6500..6901
                     /locus_tag="NK788_001970"
     CDS             6500..6901
                     /locus_tag="NK788_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCH6770296.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(6947..7897)
                     /gene="yidC"
                     /locus_tag="NK788_001971"
     CDS             complement(6947..7897)
                     /gene="yidC"
                     /locus_tag="NK788_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCH6770297.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         2521 a         1300 c         1728 g         2357 t
ORIGIN      
        1 tagttgtagg aatatcatag ggattaatgt ggatcacgct gctcgataag ttagaccatt
       61 ttgacggatc gcttatgtgg ttcgtctttt tttctgttct tttattggta actggtattt
      121 ttgaggtatc tttgtctatt caagatataa agctttaaag agctgttata atagcttttt
      181 tggtctaaaa aacctttgac aaatgattaa taattcacta ttcttgtgtt gtggttataa
      241 ccagtaaaga agaggtgatt tttatggaga gctcaaataa aggaaaatca ttgtattatc
      301 aactagttga tttattgaag gatcgtatcg aaacacagat gactccgcat gataagctgt
      361 tatctgaaag agaactttgt caacactatg gggtgagtcg taccaccgta cgtctggctt
      421 tacaggaatt ggaaaatcaa ggttatattt accgaagaca tggaaaaggt acctatgtct
      481 ctgatttgtc tgataagtca tcgaacttag ctggagcata tagctttaca gaagaaatgc
      541 gcgaaatagg gcgcgtccct aagacggtca ttttagaatt tagacaaatg gaagctaata
      601 agagaattgc taagaatttg aacatccatt taggtgaaac tgtatttaaa cttaaaagat
      661 tgcgtttggc agatgaagag ccattgatgg ttgagcgttc ttatctaccg gcgaaactat
      721 ttatgggact tgatttgccg ttacttgaaa gtaaaccgtt gtatgatgtg ttttcacagg
      781 attatgaaca acaagttcaa ttagctgatg aagagtttta tgcaagtatc gtgcaaaaaa
      841 aagatgctgt tgtgctacga atcgctgatg gcgctcccgt cttacatctg acgaggataa
      901 cctataacgt gaaaaatgag attatcgaat atacagatag tgtagcaaga gcggaccagt
      961 ttcattataa aattcggcat atccgaaaat gattgagagg gagaacaaca tgtttgagct
     1021 atcgaagaac aaattaaaac aaataggtgc agcaatcacg acacaagaaa taaaacaaca
     1081 accagatctc tggaaagaaa caatcgatag attgatgcaa agccaagaaa atttagctct
     1141 attttttgat caaataaaaa aagaagcaaa cggaagaaga atacgtgtta tttttacggg
     1201 tgcaggaacg tctcaatata ttggggatac gctcttaccc tatctaaatg aacatggaga
     1261 caatcagttt tttatcttcc acagttttgg cacgacagat attgtagctt caccaaaaga
     1321 atttttgttt ccaaatgaac cgactttatt agtttcattt gctcgcagtg gcaacagtcc
     1381 agaaagtgta gcggctgtgc aaattgcaaa tgaacaagtt gaaacattgt ttcatttgac
     1441 gattacttgt gctaaagagg ggatgttggc aaaaaacgca caggatgata aacagaatta
     1501 tctgttattg atgccagaga gagcaaacga tgcaggtttt gctatgacag gaagttttac
     1561 gtgtatgtta ttgagtgctt tatggatctt tgatcaaaca gcaatggaga aaaaagaaag
     1621 atatcttgaa caattagtta aaatcggtca agatcttctt tcaagagatc aagaaattca
     1681 aaagttatta cctgattttt ttgaacggat cgcttatctg ggttcaggaa gtttggcagg
     1741 tgcgacaaga gaagcacaat taaaaatttt agaattaaca gcaggtaaag ttgctacaat
     1801 ttttgattct tcgatggggt ttcgtcatgg accaaagtct tttttaaata gcgagacact
     1861 cgttgtaggt tttgtgagca atcaaccata tacgcgaaat tatgacttag atattttaga
     1921 ggaaataaaa gaagatcggg tcactgccaa tgtcattgcg attgcgcaac cgggtactat
     1981 tgatttctca ggtatgactg tgctattgga agactcggtg agtaaattgc ccgatgccta
     2041 tcttgcatta gcgatgatta tgactgcaca aatcattgca ctctatactt caatcaaagt
     2101 agctaataaa ccagatacac catctccaac aggcacggtc aatcgagtag tcaagggcgt
     2161 cattattcat aagtatgaaa ccgaaggaga aatgacagat gaatgaagaa aaaaataggg
     2221 caatggagag actatcaaca aaagatggga ttattagtgc attagcgatc gatcagcgtg
     2281 gtgctctaaa aaaaatgatc aaatcgttgg gaaaagagcc aagtgaagac caaatcagtg
     2341 aattcaaaga actagtatca gctgagctga caccctatgc ttctgctatt ttattggatc
     2401 cggaatatgg attgccagca gcaaaagtca gagcaaaaga gacggggtta ttgttagctt
     2461 atgagaaaac cggctatgac gcaactacgc ctggtcgtct acctgattta ttagcggatt
     2521 ggtcttctct cagattgaaa gaacagcaag cggatgcagt aaaattctta ctttactacg
     2581 atgttgatga agcacccgaa atcaatcatt tgaagcatgt gttcatcgaa cgtttaggta
     2641 gtgagtgcga aggagaaaat cgtcccttct atttggagtt agtttcttat gatgcacaaa
     2701 taagcgatag ttcttctcca gagtatgcga aagtcaagcc ccacaaagtt attgagatga
     2761 tgaaagaatt ttctgatcca aaatataaag ttgatgtgtt gaaagtagaa gtaccagtca
     2821 atatgaatta tgtgaatggc tattccacag gtgaatttat tcacagcaaa gaagaggctg
     2881 ctgccttctt taaggagcaa agtgaagcga ctccattacc attcattttt ttgagtgggg
     2941 gtgtttcaac agaattattt caacaaacac tgatttttgc tcatgaatcg ggttccacct
     3001 tcaatggtgt attgtgtgga cgtgcgacat ggaaagatgg ggtcaaagca tacgtcgaaa
     3061 atggaaaaga cggagctatt cattggttaa gaacaaatgg gagagaaaac atcgagactt
     3121 taaataaagt aataaatgaa accgctaccc cttggttcaa taaaacaaaa agcaaaaagg
     3181 tgaagatgaa atgattacga cagttacctt gaatccttcc atcgatatcg cgtatacaat
     3241 tgaagaatta aacctcgaca aagtcaatcg agttgaaaca gtcaataaaa cagcgggagg
     3301 aaaaggagtg aatgtggcga gggttctaaa tttattagga gaagaggtga tagctactgg
     3361 tttaataggt ggccagcacg gtgaatatct aaagaaccag ctagatcagt tagggatcat
     3421 gcatcagttt gttccaatta aaggagagac aagggattct attgctatcc tgcatagttc
     3481 gagacaaaca gagcttttgg aacgaggccc cttcataaca aagaaagaac aacagacatt
     3541 taccgatcat ttcgagcagc tcctcgaaat atcagatatt atgacgatct caggtagttt
     3601 accccaagga tgccctcttg attactataa gcaattggtt gctttagctg ataaaaaagg
     3661 gaaagacata ttactagata cttctggtaa atttttgctt gaagtattgg aaggtaagga
     3721 gaagccacta cttatcaagc ccaatctgga agaattagga gaactgattg gccaacgtat
     3781 ctgttcttcg attcaattag aaaaaattca gtccttatta gctcatccaa tatttgaagg
     3841 agtgccatgg gttgttgttt ctttaggaga gagaggtgct gtggcaaaag ttgctcagtg
     3901 tttttacgaa gtcattattc ctaaaatatc agttgttaat cctgttggtt caggtgacgc
     3961 gacccttgca ggatcggccc atgcgttatc aaaaaggcaa tcaatcgaag agattctcgc
     4021 attttctatg gcttgtggta tggcaaacgc gcaacaagag aaaactggct ggatcgaccc
     4081 tatgaaagtt acgatgttga tgaaacacat tcaagttaag aaaatcaaat gaacttaaat
     4141 ataaaagaaa gcgttttctt tagttgggaa ggaatttgtt cttagcttaa aaatgaaata
     4201 taagaaagaa aaaatgaaaa cgttatcttt gaaacagaag ggatatttga tgatgtcaca
     4261 gttttaattg aacaataaat agaaaaaata gataaggaga atgcatgatg gcaattattg
     4321 gtgtaagaat cgatggacgt ttgatacatg gacaagtagc aaatctttgg acaacgaaac
     4381 tgaatattag tcgaatcatg gtcgtggatg atgaggtagc cacaaacaca attgaaaaaa
     4441 gtggattgaa gttagcgact ccagcagggg taaaattaag cattttgcca atcgaaaaag
     4501 cagcaacaaa tattttagct ggcaaatatg actctcaacg agtattgatc gtagcaaaaa
     4561 agccagatcg aatcttatct ttagtagaaa aaggagttcc tatcaaagaa gtaaatgtgg
     4621 ggaatatgtc acaaacgaat gaaacacggt ccattacaaa atcaatcaat gtggtagatg
     4681 cagatatcga agtatttaaa aaattagatc agcttggtat acatttaatt gctcaaatgg
     4741 taccaaacga taaagcagaa gactttatga atcttttaat caaataggag gaatgatcca
     4801 tggatattca atggtggcag atcttattat taacattata tgcaggttat caaattttag
     4861 atgaattaca gatctattct tcaatgagtg cgccagtgtt tgcagggtta tatgcaggtt
     4921 tagtaatggg tgatttaaac gcaggtttga ttattggtgg cagtatgcag ttgacagttt
     4981 taggagtggg gacgtttgga ggcgcatcaa aaatagatgc caactctggg acgattttag
     5041 caactgcctt ctcgatttct cttggaatga atccagaaca agcgattgct gctattgcag
     5101 tgcctgtagc aagcttaatg atccaattgg atattttagc tagattcgct aacacatact
     5161 ttgcccatcg tattgaccgt cacgtagaag acatgaatta tcaagcaatc gaacgcaatt
     5221 tcttaatggg agctttacct tggtcgctct ctcgtatgat tcctgtattt ttagctttag
     5281 catttggtgg tggattagtt gaaaatgttg tttctgtttt aaacgggaat ctaaaatggc
     5341 taggcgatgg actttcagta gcaggagctg ttttaccagc tgttgggttt gccattcttc
     5401 tgcgctattt accagtaaaa aaacatctat cttatcttat tttaggattt acgatcacgg
     5461 cattattagc aacgttattc tctaatgttc aattattagg aacaagtgtg gcaagcgttg
     5521 ttaaagatct tccggcaact ttcaattcgc tgccgatgct tgctgtagca ttgattggtc
     5581 tagcactagc aatgatcagt tataagaatg atcaaaacaa agaccatcaa ccattagcta
     5641 gtgagccatc acattctgtt gagggggaaa tcgaagatga cgaaatctaa ttacaaatta
     5701 acaaaagaag attttaagca aatcaatcga agaagtttgt tcacttttca attaggttgg
     5761 aactatgaga gaatgcaggg ttctggctat ttatatacta tcttgccaca actaagaaaa
     5821 atatatggag acaatacacc agaattaaaa gagatgatga aaacacatac gcaatttttt
     5881 aatacttcta atttttttaa tacaatcatt actgggattg atcttgcaat ggaagaaaaa
     5941 gagggaattg cttcaaagga aaccgtatcg gggctgaaag ctggattgat gggttccttt
     6001 gcagcgattg gagattccgt atttgttgct ttgattccaa cgatttttgg ggctttggcg
     6061 gcgaatatgg caactgatgg aaatccgata ggtgtgttta tatggatttt tgctcagatc
     6121 gttgtcatgg tttttcgttg gaagcaactt gaatttgcat acaaagaagg aatttcctta
     6181 gttactacta tgcaatctcg cttaactgca ttaacggacg cagccacttt attaggggta
     6241 ttcatggtag gggcattagt tgcgacaatg atcaatgtta aattttcatg ggcaccaacg
     6301 attggcgatg tgacattgaa tatgcaaaac tatatcgata tgatcttacc taaaatttta
     6361 cctgctggga tcgtcggtgg tgtttattgg ttattaggaa agaaaaacat gactcctact
     6421 cgagcgattt ttatcgtatt aattgtgtgt gttgctttat ctgcattggg tgtgatttcg
     6481 aaataaggga gagcgaaaaa tgagtagaca attggtattg attagtcacg gaaaattttg
     6541 tgaggaattg aaaaaaagta cagaaatgat catggggcct caagaggata tctacacgtt
     6601 acctttatta ccagaagatg gcactgaaac atacaaacaa aagtttctca ctgtcacaga
     6661 ttcgttaaat gattttgtgg tactatgcga tcttttaggt ggtacacctg caaatatcat
     6721 ttctaaacta ataatggaag gacaacagat tactctttat gctgggatga atcttccgat
     6781 ggtcattgaa tttatcaact cacaaatgat tggttcagag cccgagtttg ttcgtgcagg
     6841 atgtgaaagc attgtctctg tcaacgaatt aattaatgga atagcagaag aggaagagta
     6901 acaaaaaaga tgagggctta gctctcatct tttttgtcct atgtttttat ttccgcttct
     6961 gctgctttcc tgcgttgcgg cctttgttgt ttgtactgtt ttttggttgt ttgatttgtt
     7021 ttgttgcttg tttgttgtta gctggctttg ctgattctgt cacgtctttt cttggagtga
     7081 cgacttgttt tggcgggttt tgtttcaact cttctgccac ttgtgctttc aaacgtggtt
     7141 tcatcataac gtttgtgatg aatgtttgca gacagctgaa cacaccaccg actacccagt
     7201 aaagtgtaac accggctggc gcactgatcg acatgaagac gatcatagca ggagagacga
     7261 ttagcatcgt acgcatcgtc tttttctgtt cttcaggaat accgatcgtt gagatgtatc
     7321 cttgaagcag ataggcgacc cctgcaatga caacaagtac catacttggt gagcctaaat
     7381 cgatgccgta gaaagatgat tcccgaatac cttctgtata acgtgccgta aagtacaagg
     7441 cagagaagat cggcatttgg atcaatagag gcagacagcc gattccgccg gtcatactca
     7501 atccgttttc gcggtatact tgctgcattt ctgcttgtgc tgccatttgt tcttcacgtg
     7561 ttgttgcagc tttcaatttt tgttgggctg cttcaacttg tggttttaac gcttgcattt
     7621 tttcagattg gatcattgtt tttttcgatt gactaatacc tagtggcaag atgatgatac
     7681 ggacgatcac agtcattgca ataactgccc agccataaga ccaattgaaa ttctcaacta
     7741 gatatgtaat cgcttggccc ataggatgga cgagtactcg ataaacgatt cccgaagtgt
     7801 cagggtttcc gtttgaatcc atccgcacac agccagataa aaagagcagg atactgacaa
     7861 gtccggatcc caataaccag tttttccatt tattcataga atactt
//
LOCUS       DAHUWM010000127         7849 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHUWM010000127 DAHUWM010000000
VERSION     DAHUWM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.129"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            84..785
                     /locus_tag="NK788_001972"
     CDS             84..785
                     /locus_tag="NK788_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCH6770298.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1394..2410)
                     /gene="tsaD"
                     /locus_tag="NK788_001973"
     CDS             complement(1394..2410)
                     /gene="tsaD"
                     /locus_tag="NK788_001973"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCH6770299.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2407..2871)
                     /gene="rimI"
                     /locus_tag="NK788_001974"
     CDS             complement(2407..2871)
                     /gene="rimI"
                     /locus_tag="NK788_001974"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCH6770300.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2878..3420)
                     /gene="rimI"
                     /locus_tag="NK788_001975"
     CDS             complement(2878..3420)
                     /gene="rimI"
                     /locus_tag="NK788_001975"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCH6770301.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3404..4126)
                     /gene="tsaB"
                     /locus_tag="NK788_001976"
     CDS             complement(3404..4126)
                     /gene="tsaB"
                     /locus_tag="NK788_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCH6770302.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4317..5297)
                     /locus_tag="NK788_001977"
     CDS             complement(4317..5297)
                     /locus_tag="NK788_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6770303.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5321..6805)
                     /gene="galT"
                     /locus_tag="NK788_001978"
     CDS             complement(5321..6805)
                     /gene="galT"
                     /locus_tag="NK788_001978"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCH6770304.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6817..7806)
                     /gene="galE"
                     /locus_tag="NK788_001979"
     CDS             complement(6817..7806)
                     /gene="galE"
                     /locus_tag="NK788_001979"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCH6770305.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2193 a         1695 c         1394 g         2567 t
ORIGIN      
        1 ctaatgttgg tatgttaaaa ctttacaaat tctttaaaaa aagtgacaat aaatatagtg
       61 aacaataagg agaggacttt ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac
      121 tcagatatca acctgatttt gaacatttct cggatgaaga gttccaacag atcaaggaac
      181 atactgttct tcgttcttat aaaaaaggac agatcctttt cgacgatggt gatcccagac
      241 aacggttgta tatcttgacc aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa
      301 cttattacta ctatgactat gtgaatacaa ataacctttt tcctatgtca ggaatgtttg
      361 gagaagagac ctacacttat acagctcaag cgatgactga tattgaaaca ttctaccttc
      421 ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc tatatgatac
      481 aaaaaatttc taaagtagtt gaaatacatg aattgcgtat ccaaatcgga ttgacctcta
      541 gcgcctttga ccgggtaaaa caaaatttat tcattttgaa agaagaattg ggcgtaccaa
      601 caaaagatgg tcatatcacc attccttatc cgatcacctt aaaagaatta gcagtgaata
      661 gcggcacgac acgtgaaact gctggacaag taatcaaaca actacgtgat tccgggctgt
      721 tagattatga caaaaagcgt tttactttct gtaagacagc tactactcca gctatgagag
      781 aataacgcga acaaaaataa gcagtgaccc tgataaatat cagggtcact gcttattttc
      841 ctccctaaaa cttctgaaag atccggcagt tttagatcac tggacaagac tcaaagattt
      901 tctatgtgtg tttcagttat gtaatattca ctgtttgcaa aaaatactgt tagattaact
      961 agatgaagcg tcttgtcctc atccgatcta gattattcga tattcctcga caataaacga
     1021 ttactcagtg agggcaatca gaggaacgga taatgataaa taagctgtaa aaaatagcaa
     1081 aataacaata atgattaaaa gtatgtaaaa aatagggctt tgacaaaaac gtgtcacagc
     1141 cctatttcga ttaaacggtg tgtacctgtc aactcttcgg tcacaagtca taatgttcat
     1201 aaacatgagg agctgttttt tgaaattgtt ccttgtgaat cagagttaaa cgacaggctt
     1261 acgaactctt gattaaacgg cgttcaaaag cttgcatacg cggtaataag aacagccagg
     1321 tcaaaaacat gagaagccgt ttttcctggg ctgttcttat tacttgatgc agggcagctt
     1381 tttcacaacc tctttattca gataaaacca agcttggatc agcattcaac tgatagctag
     1441 caaaatttct tttttgcatt tccacatgtg ctgctgctcc aatcattgca gcattatctc
     1501 cacaaagtcg aagaggggga atcactagtt ctacctcagg gagtttagct gccaaagcat
     1561 cctgcaatcc ttcacgcaac ccttgattgg ccgctacacc tccagctata atcagttgct
     1621 tgactggata attttgacaa gctcgaagtg ttttagtaat caatacatcg atcacacttg
     1681 cttgaaagct agctgctaaa tcatttttgt ctagatcttc tccacgctgt tgtgcattat
     1741 gcaccaaatt gataaatgca ctttttaatc cgctaaaact gaaatcataa ttatcttcat
     1801 ggatcatcgc tcgcggaaaa tggtaattat cttttccctg atgagctagc tgatcgattt
     1861 cttttccgct tgggtaagaa agacctaata ctcttccaac tttgtcataa gcttctcctg
     1921 ctgcgtcatc tcttgtctcc ccaatgattt catatgatcc atcttcttgc atatagacta
     1981 attctgtatg tcctccgcta acaagaagtg ccatcaatgg aaactggaat ggtttaacca
     2041 gacgggctgc atagatatgc cctgccatat ggtttacagg aataagcggc aattggtgtg
     2101 cccaagcaaa agccttagct gcagaaatac caatcagcaa cgagccgaca agtcctggtc
     2161 cataagtgac agcgactgcc gataaatctt ctgccgacac accagcttct accagtgcat
     2221 cttccaagca taaggtgatt tgttctacgt gatggcggct agctacttca ggtactacgc
     2281 caccaaaacg tttatgactg tttatttgag aagcaacgat gttagataag atctctgttc
     2341 catttctaac gaccgctaca ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt
     2401 cttcattcat ttcatcgtct ccgtttttaa ctttctgctc ataatgatcg catcttccgt
     2461 cggattgcga taatatgatt tccgtttacc taatatacga aacttttctg attcataaag
     2521 ttttcttgct gcttcattag atagacgtac ttctaaaaaa acatgcagcg aatcttttat
     2581 ttgttccgta atcagtaatt gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac
     2641 tgcaacagca atatttgtga tctcgacttc atctattact ttcgagtatc caataaagcc
     2701 acagatctgg ttatcttcag ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc
     2761 caaaaactgc attttcgtcc aaggtgaacc atgtggataa gcattttcgc taatcatcca
     2821 tacgcattct gccaagtacg cttcatcaaa atcttttttt ctgcaaatca actaatctca
     2881 ctcattttct aaaagtttta tcatatccaa cgcatcttct tttgtttcag tgtaatatct
     2941 ttttttgatc gttgttgctt gaaaccccaa tttccgataa agtctttgtg catcttgatt
     3001 actcatacgc acttctaatg acatcgtctc acagcgattc attatagcaa agttctcgat
     3061 ttcgtcaata agcagcgaac cgattcgttt acgctgatat gccggtcgaa cggcaatatt
     3121 cgtgatatgg caatctaaac cgaacgctct tgaaccgata aaaccaatca ttcttccatt
     3181 atcaaagata cctagatata gatgaggaag cggtgacttc aattctgata taaaggcact
     3241 ttttgtccat ggcaattcgc cattatagac ttctcgttcg acatccagca gctctttgat
     3301 atcttctgta gtcaattctc ttagtacgta acgacgatcg atttgttcga ctgttttttc
     3361 tgtataaatc attttatctt gaaaaaagct tctggcatac gctctaaatt ttttcaacat
     3421 aactttcgtc tccaggtgtg tgcgtctcca accatttttc ttctgcttct actcgtttta
     3481 aatagcgagg aagaaaagct tgtttctctg caactggttc aactaaagca cctaattgag
     3541 ccacagttac accacttgga atatcccatg ctggaactgt attaatcatc gcactaggca
     3601 atatttcctt tatttcagag aagaatttgt gacaatcact cccgacgaaa taaatagctt
     3661 cattcagctt ttgtagttgt gtgaacaaat cagaagcaga aatatgtaca tcttcaagta
     3721 cagtttctaa tctattttct ttccagcggt atgccccagc gtatacattt tttcttcgag
     3781 catcaaacat gggcacaata atattatcca caccgatgca attagccgcg atggttttca
     3841 gactagatac tcctactagc tctttatcta atgtataagc aatcgttttt gctgtcgtta
     3901 cacctatacg aagccctgta taagaaccgg gaccatcaga aaccgcgatt cggtcaatgt
     3961 cttttggact tatttgaaga tctgcaaaca attggtcgat tgcaggcatc agtgttacac
     4021 tgtggttgcg tttgatattt gtttgtattt gtccaagaat ttgttcgtct tctaccacac
     4081 cgatcgccaa agtttggttt gcagtatcta ttcctaatgt aatcatatat ttgatccttt
     4141 ctgctatgac attctcgcct tccattgtag catacttttt tattctgagg atgaaaatac
     4201 caaaggcaaa actagtacaa taatacatga ttatctgctg gcgggcaaat tttgcccctt
     4261 cctgaaacac aaaaaagctg cggtatcact ccattttccc acagcttttc catcttttat
     4321 ttcgttgatt ctcgaataat caactctgtt tccacaataa tctttcttga aaccggtgct
     4381 ttggaattac ttaattgaag aatcgtctcc actgctagct ctcccatcca ttcggtataa
     4441 acttttacag tagaaagcga gggcgttaca tatttcgcta cactgacatc attgaagccc
     4501 atcacagaaa tatcttctgg taccactaaa cctttttctt gaatcgcttt cagtgcgcca
     4561 atcgctaatg catcattagc tgcaaaaagc gcatcaggaa catgcggatt cgtttttaaa
     4621 tattgcttca ctgcttgata tcctgactcg acagaaaaat ctgcttcgat aattggacct
     4681 tcgtcgtagc ctttttctgc caaaacttgt tgaaatgcca taattctggg atcttttagc
     4741 cgctgatggt ttttcttcgt atattctgtt ccagcaagca tagcgatatg ttgcgcttta
     4801 tgggagacaa agtgttcaat gactttttga acacttgact taaagtcgac aactagagag
     4861 tcgattcctt gagaagtgcc gtcagaatct accaaaagca aggcattttg ggatcctttc
     4921 atttctaata actgagcttc gtcgaatttt cctagtgcga tcgttccatc tgccggaaga
     4981 tcactaatgg aatcccacgt ttctttcaat aaagtaacgt ttgcttcttc tgcctttttt
     5041 tcgattccta ggcgaataga taaataataa agatcttcaa gctcttccgt atcgtcgtac
     5101 cactggatca ggcgaagcgt ttgcttagta tttgctgcat ttttacgatg tttcgtataa
     5161 tttaagcgtt ctgctgcttc aaaaattttt tgctttgtgg ctaagccaac ggaaagatca
     5221 ggatcatagt tgagaactct tgaaacagta gcaggagaaa ctcctgccaa ctgcgcaatg
     5281 tctttaattg ttgccatttg tctctcctcc ttttttacgt ttataaaatc gcagtaaatc
     5341 gttcaaacgc tgcctgtcct ttttcatctc gcttgaatac tccagcgtct tccagtacac
     5401 gtgcaaaaac ctgacctaca gatttttgta cgacatcggt gaccgtatct ttcgtcaaat
     5461 caggatgttc tttttttaat tgttctgccc acggacgatg atattcagcc atctggtttt
     5521 cttctcctaa taagaaatgc tctacttcta ctagttctgg tttcaaacga ggtggtaaaa
     5581 cagcaagccc cattacttcg atcaacccaa tgttctcctt tttgatatgt tggacatctt
     5641 tatgtggatg aaaaatccca tcaggatgtt cttctgaaac attgttgtct cgaaggacaa
     5701 ggtccaactc aaagtaattg tcccttcttc gtgcaattgg cgtaatcgta tggtgtggtg
     5761 taccatctgc tgaaaaagct ctgatagata ctgtttcatc cgaatatgct ttccattttt
     5821 ccaatactga gttagctgct tcaataagca agtttttctc tgttgattgc aaacggatca
     5881 cagacatcgg ccatttcacg actcctgcga tgacttgcgg ataatcttct agtttgataa
     5941 gtttttctat cggtgctttt gccataggaa attcatgacg tcctgcctga taatgatcat
     6001 gagaaagaat cgatcctcca acaattggta agtcagcatt cgaaccaaca aagtaatgag
     6061 gcaagacttc agtaatccga agcagtctag caaatgtttc ctttgtgatt ttcatcgggc
     6121 ggtgttgttc tgataaaatg atcgaatgtt cattgtaata agcataaggg gaatattgga
     6181 atccccagct ttcaccgtct agattcattc gaataatccg gtgattggtg cgggctggat
     6241 agttcaagcg acctctatat ccttcgtttt ccatgcaaag catgcaggta ggatagtcta
     6301 cttttaccgc ctcgcgttct gctgcgattt gttttggatc tttttctggc ttagataaat
     6361 tgattgtgat ttccagttca ccatactccg actctgccgg aaatttgatg ttttttgcga
     6421 tggctcttgt tttgatgtag tcatttttct tacttaaatt aaagaaatac tcagttgctt
     6481 cctctgggct tttcgaatag tgctgtgcaa agaatgcatt gacgactgaa ggtggcggtg
     6541 tgagaaaatc cattaattgc gcttcaaaaa tctcttgttc tgctggcaat tctgaaagaa
     6601 caccattttc tcttgcctta gcgacaagct gatccaataa gtctacagat tcttccttaa
     6661 ctggtcttac ttcaatgctt tctagagcat cttctcctac catccctaaa agacgattct
     6721 gtaaatataa gcggtccatt tccatccatc cgccagcttc gatcgcaagt gtggcaaaat
     6781 cacaaatcgt ttgactgatt gacatatctc attcccctat tcttcataac catttggatg
     6841 gctgacatgc caatcccatg ctgttttgat aatcgctttg atatctgtgt attcaggttt
     6901 ccagcccaga atctctctcg ctttttcact tgaagcgact aatcggctag gatctccagc
     6961 tcttcttggt gctatttctg ctggaatttc ttttcctgtc acttctctag ctgcttctag
     7021 catttccttg actgagtatc ctttgctgct gcctaaattg aagaagttgc tttcatttcc
     7081 ttctttcaag tattccaaag ctaaaatatg tgcagcaatc aaatcttcta cttgaacata
     7141 atctctgata catgttccat ctggtgtatc ataatcgtct ccataaacag ccaaggcttt
     7201 tctttgacca agagctactt gtaaaatgat tgggacaaga tgcgtttctg gtgtatgatc
     7261 ttctccgata gatgcatctg cttttgctcc ggctacattg aagtagcgca aagcgacata
     7321 gcgcatgcca tatgcttggt cacaccactt catcattttt tccatcatca atttactttc
     7381 accatacgga tttttaggat tagctggtgt atcttctgtg attggagact ctttcggctc
     7441 tccataagtt gctgcagtag aagagaatac gattttattc acgttgaact cgtgcatgac
     7501 ttcaagtaag atttgcatac cgtatacgtt gttgttgaaa tacatcaatg gtttttcgac
     7561 agattctcct actaaagagc ttgcagcaaa atggattact ccttcaatcg gttccttttc
     7621 aaatacggaa cgtagaaatt ctttatcccg gatgtctcct tcataaaaat gagcatctgg
     7681 atgtactgct tgtttgtgac ctgtgagcaa attatctacc acagctactt gatacccctt
     7741 ttgaactagc tggtctacag cgtgcgaacc gatataaccg gctccgccta gtactaaaat
     7801 tgtcataaaa aacactcctt tgattagtaa aattgtgttt gttgattct
//
LOCUS       DAHUWM010000128         7840 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHUWM010000128 DAHUWM010000000
VERSION     DAHUWM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.130"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(21..995)
                     /locus_tag="NK788_001980"
     CDS             complement(21..995)
                     /locus_tag="NK788_001980"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCH6770306.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="NK788_001981"
     CDS             complement(1075..1950)
                     /locus_tag="NK788_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCH6770307.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="NK788_001982"
     CDS             2080..3987
                     /locus_tag="NK788_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCH6770308.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="NK788_001983"
     CDS             complement(4528..7806)
                     /locus_tag="NK788_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCH6770309.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2208 a         1660 c         1461 g         2511 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 caacaagtgt attcctcccg taacaaatta gaaagcgttt
//
LOCUS       DAHUWM010000129         7745 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHUWM010000129 DAHUWM010000000
VERSION     DAHUWM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.131"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            450..1007
                     /locus_tag="NK788_001984"
                     /pseudo
     CDS             450..1007
                     /locus_tag="NK788_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1065..3104
                     /locus_tag="NK788_001985"
     CDS             1065..3104
                     /locus_tag="NK788_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCH6770310.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3213..4364)
                     /locus_tag="NK788_001986"
     CDS             complement(3213..4364)
                     /locus_tag="NK788_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCH6770311.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4518..5048)
                     /locus_tag="NK788_001987"
     CDS             complement(4518..5048)
                     /locus_tag="NK788_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCH6770312.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5147..5497)
                     /locus_tag="NK788_001988"
     CDS             complement(5147..5497)
                     /locus_tag="NK788_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCH6770313.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5654..7183)
                     /gene="ahpF"
                     /locus_tag="NK788_001989"
     CDS             complement(5654..7183)
                     /gene="ahpF"
                     /locus_tag="NK788_001989"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCH6770314.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7193..>7745)
                     /gene="ahpC"
                     /locus_tag="NK788_001990"
     CDS             complement(7193..>7745)
                     /gene="ahpC"
                     /locus_tag="NK788_001990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCH6770315.1"
                     /translation="GKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFVCP
                     TELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQISR
                     IFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPGE
                     VCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         2641 a         1492 c         1415 g         2197 t
ORIGIN      
        1 caacgggatt ctttcaactt atttgttatt tttttctaaa aaatattaaa gattgttaaa
       61 tttcactatt tcatttcaaa ttccattgcc taatcattag gaaaagtcta tcatgaactc
      121 gtaaatagaa tatagtaata accttgttag gtgaggctcc tatacaaaca taggctgttg
      181 cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta aggctttttc
      241 caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg atgagatgat
      301 gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt tttgtgactg
      361 acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt gtgaggagga
      421 gatgcggtga aaaaagatcg attggtggag gtcgctcgga ttcacttctg gttgcacaat
      481 acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca tatgtcgata
      541 ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga gttgatttat
      601 taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca agtatcacat
      661 ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag atcgatgcag
      721 aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg atggatggta
      781 atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc gggaggatca
      841 gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta acttcaatat
      901 tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac cttccagaaa
      961 gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat aaacaggtat
     1021 aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa agaagtgaca
     1081 agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta atagcagctg
     1141 cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta gaagctggag
     1201 aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac aaagctgcag
     1261 aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag atcttagata
     1321 aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg aatctaaaag
     1381 tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg aatacctcat
     1441 taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat ggtcaaacca
     1501 cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac aaagtaagcc
     1561 tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa ccattagcaa
     1621 caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat ggaggcgaaa
     1681 aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat gctatcaatc
     1741 aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa atcattgatg
     1801 gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc agatattatc
     1861 ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca gcagaagata
     1921 ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt ttggagatgg
     1981 cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca gatgcagacg
     2041 gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat atcaaattga
     2101 caatcgatgc gaaggcacaa aaaatagctt ttgacagtct aggaggaaaa gctgcatcaa
     2161 ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct ccaagctatg
     2221 atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat gaagaaaatc
     2281 ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct acgtttaaaa
     2341 tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa gtgttgacga
     2401 tcaacgggct taaatggcaa aaagacagtt cttggggatc ttttaaaaca acgcgtgtca
     2461 gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat aatatatatg
     2521 cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta gataaattca
     2581 tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt tctaatgaag
     2641 atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc gaacttctaa
     2701 ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc acacttgtct
     2761 atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc agcgaaacag
     2821 cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat ggtacagcac
     2881 attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg gaaatcaaag
     2941 aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat cctgataacc
     3001 aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca gcaaccaaac
     3061 gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt tctttttaag
     3121 tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa cggttttcac
     3181 gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt ttttgtaaaa
     3241 tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac taaaggtgaa
     3301 tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat caaagccatt
     3361 tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga aaaaccggca
     3421 atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata
     3481 gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc tacactgaca
     3541 aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta tacttcttct
     3601 ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac agagctaagc
     3661 ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac gatgataata
     3721 gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat ccatttgact
     3781 aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg taaagaagca
     3841 ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa aacgactaaa
     3901 atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt cactacgttt
     3961 agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat aatcccaaag
     4021 aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc aaaaacaacc
     4081 gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt tttcaaaaga
     4141 gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat ctccgaaaag
     4201 tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag caaaacacca
     4261 acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg tgagccaatc
     4321 gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat cattcctttg
     4381 tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg aaaatatact
     4441 cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat acggtctatt
     4501 ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa attgataatc
     4561 tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag catcaaactt
     4621 ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact actataaaca
     4681 ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct gttgatgata
     4741 acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa agcatagacg
     4801 atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca agaacctgtt
     4861 ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg
     4921 gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat cgcaatccca
     4981 gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg tttaatcgtc
     5041 tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca ttttaccaaa
     5101 ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa cttctgagga
     5161 tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa taacgaattt
     5221 ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca cctgtaatca
     5281 aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt
     5341 tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa atgatttcat
     5401 ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata ggataaacgc
     5461 ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt tctttttttc
     5521 aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt attcaaaaaa
     5581 acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc tgacttttta
     5641 caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct gcagtagctc
     5701 ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca ccagctgcga
     5761 aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt cttggattca
     5821 tttcgatcgt tctatctagc caatccgtat tagggagtaa gccgactaga atgaatactc
     5881 ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag agagattcta
     5941 cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg acattcgcaa
     6001 gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag aattctagaa
     6061 catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca gaattaccac
     6121 caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt gggcaataag
     6181 caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc cagtgcgcac
     6241 ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag ccaatattaa
     6301 taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg tcgatagggt
     6361 aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg taagaggtac
     6421 caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa
     6481 gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct gctggtccac
     6541 cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca atcaaagggc
     6601 cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat tcttctccat
     6661 ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt tggaacatcc
     6721 caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata ttcaatgcct
     6781 gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa tgcaatgttt
     6841 gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc cgtccactaa
     6901 tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata ccagcaaaaa
     6961 cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc atcgaatctc
     7021 ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat tcagctagtt
     7081 tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat tcaatcagtg
     7141 ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc acctaaatct
     7201 ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat tttgccggac
     7261 aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca atcagtgcac
     7321 tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt ccgtctggat
     7381 caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca tcaaaaatac
     7441 gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg atagcatctg
     7501 atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa aagacttcaa
     7561 cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa
     7621 caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata agatcttttt
     7681 cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct tcgatttttt
     7741 tccca
//
LOCUS       DAHUWM010000130         7743 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHUWM010000130 DAHUWM010000000
VERSION     DAHUWM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.132"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            128..808
                     /locus_tag="NK788_001991"
     CDS             128..808
                     /locus_tag="NK788_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCH6770316.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            898..1932
                     /locus_tag="NK788_001992"
     CDS             898..1932
                     /locus_tag="NK788_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCH6770317.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1946..2281
                     /locus_tag="NK788_001993"
     CDS             1946..2281
                     /locus_tag="NK788_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770318.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2265..4745
                     /locus_tag="NK788_001994"
     CDS             2265..4745
                     /locus_tag="NK788_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770319.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4801..6360
                     /locus_tag="NK788_001995"
     CDS             4801..6360
                     /locus_tag="NK788_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770320.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6357..7721
                     /locus_tag="NK788_001996"
     CDS             6357..7721
                     /locus_tag="NK788_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCH6770321.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT         2556 a         1412 c         1553 g         2222 t
ORIGIN      
        1 attttattgt ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa
       61 ttcatgagga ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg
      121 atttattatg aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg
      181 cttggcaaca ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac
      241 tgaaccaaca aataccgttg agcctcttaa ccctactgat ccttcaaaac cagtagagcc
      301 agcagatccg gaaaatccag caactggcca gacaggttct ttaacattgg atgtagttcc
      361 tgaattgcca tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc
      421 ttcaaaaaac gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg
      481 ccaagcacaa gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt
      541 tttgcaaggc gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga
      601 atccactgga ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc
      661 aatatttaca gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc
      721 aaatatcaca ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg
      781 gaacctcgtg gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa
      841 aatactatag gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg
      901 aatcaaagta aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt
      961 ccgtcaatca gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca
     1021 gagaaccaaa tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat
     1081 cagacactag aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct
     1141 gtcaatccgg cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg
     1201 ttagatgaga cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc
     1261 aatgtatcag cacatgcaga aacaactata cctatagaag tgaaaatccc agcaaaaagc
     1321 tttaagggac gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa
     1381 aatgctacag aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta
     1441 caagaaagtc aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag
     1501 gtcaacgcaa agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc
     1561 aatttgatat ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct
     1621 aatgaatttt tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat
     1681 aaagcaaaag ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa
     1741 tggcgattta cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat
     1801 tccgtttttg cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca
     1861 gttctagtgt tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga
     1921 gaaacaacat gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc
     1981 aagtttgctg ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac
     2041 gattacattt tatgatctaa gtccagttcc gcatccaagt ggttcagtca atggcgcatc
     2101 aaaacagcag acacagctac cgatttataa agaatctaat caacatggcg tattcccgga
     2161 gacgaatgaa gaaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct
     2221 tgcgtggttg ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata
     2281 aattttcatt ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct
     2341 tttcaccttc ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca
     2401 cgtatgaaaa tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg
     2461 tccaactaac tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt
     2521 caaatgatcc ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa
     2581 caaatgtact tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca
     2641 atttgccgga acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct
     2701 acttgtatat tgccggtgcc cccatcaagg ttactagcgg tagtttatta tcagcaggaa
     2761 gccatacctt gcgttttggg atggggtcgt gggatacaaa tactctattg gcagatatta
     2821 ttgctattat tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta
     2881 cagctaatgt cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa
     2941 ttttgaccaa gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt
     3001 tttatgattc gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg
     3061 caggtagatt ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt
     3121 ctgcaagtgc agagataaat acgtggaata gttacatctc aacaagtgat tcaacaggaa
     3181 aatataatac atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag
     3241 gctctaccaa tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc
     3301 catttagtcg ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag
     3361 caatactgac tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt
     3421 acagtgggaa agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc
     3481 agtacaagct agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa
     3541 agctcctttc ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa
     3601 agggttcgat ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga
     3661 cagatcaact tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta
     3721 cactacctgc attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac
     3781 ttactaacat gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca
     3841 ctcttcctca aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa
     3901 accttagcag acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc
     3961 aagaaaatgt gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca
     4021 cgatctcact tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa
     4081 ctatcgcaga taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa
     4141 taggagatac cattcatctt acttacaact atcaattaaa tactgtagat acttctgtac
     4201 aatccgtcag catactgaca aaggcagctg tactttccag caatattaca ttagcagatg
     4261 gtgctaagct tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac
     4321 aagaattaac attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac
     4381 cgttgaagac aagttattat gaagcaaaag gcgatttttc ttttgatgtg cgggatactc
     4441 ggcttccaag cacgtctcct tggcaactga cagggacatt gacttcttta tttaagaatg
     4501 atcaaggtca agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaaacaat
     4561 tgattcagca aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg
     4621 ttctcgttga ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg
     4681 ctcaacctgg cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca
     4741 gctaagtgat tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg
     4801 atgagaagga aacttctgct gttaaaatca gaaatgcttg gaggagtgga cttggaactt
     4861 ttcagtttgc ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag
     4921 gaaaaaggag agatttccgt taataacgta cttacccgct tatcagttga tcgggctacc
     4981 ttgaaagaag atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt
     5041 gttatccaag aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt
     5101 tattatcatt gctacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa
     5161 aatggctcat ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg
     5221 tctcgcagag tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc
     5281 gggcggctga ccggtccaga aagtcaaatt tgttattttt atttccaatt attttggttt
     5341 ggacgtcctt atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta
     5401 gatctgctgc aaaaagagtt tagttttact attacagaag aaggacgatt gaaatttgaa
     5461 atatggcttt ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat
     5521 agcaatcttc ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt
     5581 ttgacccgtt acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt
     5641 tatcttttca tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt
     5701 ttgataagcg aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa
     5761 tcgatagaag aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg
     5821 ttgacagtga tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt
     5881 ggccaagaag gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta
     5941 tgtgaaagat tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa
     6001 aatgcccgct ttgaattatt tgctcgctat gcatctattt tgcggacgct gatcaagcag
     6061 ttagaattac gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc
     6121 gtgaaagaaa tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac
     6181 gagaaagaaa agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag
     6241 aagtccgcta aagtttttgt attcactagc aacgaatata attatgattg cccctatttg
     6301 aaccaatttt taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga
     6361 aaatcgtgat cattggtgct tcccatgcag gcatcacagc tgccctaaat ctaagaaaac
     6421 ttcaaccgaa agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg
     6481 tttcaaatgg aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc
     6541 ataacatgcg gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg
     6601 aacttgatcc agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca
     6661 taacctatga taaattaatc gtagcaacag gttcctctcc tgtcaccttg tataaacaaa
     6721 ttgaagctga aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg
     6781 ctttaaaaga agcaaaagaa gtagtgatct tcggtgctgg ttatattgga ttggaattag
     6841 cagatgcttt gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt
     6901 tatcaagata tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc
     6961 aaatcaattt ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag
     7021 tatccgtaca acttctatct caagcaataa aggcagatat ggtcattttt tctgcccaaa
     7081 ctcgtcctaa tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga
     7141 tagtcaatga atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc
     7201 cagtcagttt tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac
     7261 atatggcaag agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac
     7321 aaaagataac agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag
     7381 atgaagcgcc attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc
     7441 cacaatacga tgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat
     7501 tagaaataat tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc
     7561 ttgcggatat cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat
     7621 ttctgtctga acacacctct cgttttcact acctcaatga gttagctttt aaagtactag
     7681 ctaagagagc aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta
     7741 ttg
//
LOCUS       DAHUWM010000131         7727 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHUWM010000131 DAHUWM010000000
VERSION     DAHUWM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.133"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(18..245)
                     /locus_tag="NK788_001997"
     CDS             complement(18..245)
                     /locus_tag="NK788_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCH6770322.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(242..760)
                     /gene="msrA"
                     /locus_tag="NK788_001998"
     CDS             complement(242..760)
                     /gene="msrA"
                     /locus_tag="NK788_001998"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCH6770323.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(780..1493)
                     /locus_tag="NK788_001999"
     CDS             complement(780..1493)
                     /locus_tag="NK788_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCH6770324.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1496..2392)
                     /locus_tag="NK788_002000"
     CDS             complement(1496..2392)
                     /locus_tag="NK788_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCH6770325.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2550..3392)
                     /locus_tag="NK788_002001"
                     /pseudo
     CDS             complement(2550..3392)
                     /locus_tag="NK788_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
     gene            3524..4177
                     /locus_tag="NK788_002002"
     CDS             3524..4177
                     /locus_tag="NK788_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCH6770326.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4240..4767)
                     /locus_tag="NK788_002003"
     CDS             complement(4240..4767)
                     /locus_tag="NK788_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCH6770327.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4787..5734)
                     /locus_tag="NK788_002004"
     CDS             complement(4787..5734)
                     /locus_tag="NK788_002004"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCH6770328.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5746..7632)
                     /locus_tag="NK788_002005"
     CDS             complement(5746..7632)
                     /locus_tag="NK788_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCH6770329.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         2009 a         1581 c         1353 g         2784 t
ORIGIN      
        1 aagtacaaaa ctatctctta tatgtcatga ttattttcta aatatttttc ccatgcttcg
       61 tcaaagatat ccatggacgg caaatagtcg acattcagtt ccagataatt agaaatttct
      121 tcgtagctag agctgtgctt aggaaattgg atgtctttac cggcttctgt agcgaaaaca
      181 gataaagcat catgtgtgcc accgcctcgc agtgtcatta aataatgaaa aaagcttcga
      241 ttcacccttc gttcctccaa ttttcttcga taaaggcagc ccggcgtgca tggtttcttt
      301 taaaattttc aggatgcttt ttataatact cttgatggtc ttcttctgct agataaaatg
      361 gttgggctgg ttcgattttt gtaacgattg gctgttcgaa acgtccactt tcagctaact
      421 gtttcttact tttttcagca atgatccgct gttcttctgt caaataaaag atcaccgggc
      481 gataattgtc accgcgatcc tcgaattgtc cgaaagcgtc agttggatca gtttgctgcc
      541 agtaaagttc tactaattgt tcatatgaaa tcaatgttgg atcatagata atctccactg
      601 cttctgtatg tccagtcgtt tggcttttta cttgttcata agtcggatct ggtacatgcc
      661 ctccagtata tcctgacatc actgtatgga taccaggaag cgtgtcgaaa ggctggatca
      721 tacaccagaa gcagcctcct gcaaatattg ctcgttccat tatgattccc cctctaatac
      781 tactcatccg atgatttttt attttctttt gtcttgcttt cagtactgct ggtgctagtt
      841 ttgttggtat ttgagttgtt tgaagcatca gaggtatctt ttggcaaata tagactaaac
      901 cgaatgtcat catttactaa gtcgatttta tctgctttaa tatacatccc gttttgcatt
      961 cggaattgat ccaacctaag catgatagtc agatcatctg tattgacttc gatccaatct
     1021 ggtaatttat agtttctctt gatcatattc atcacatctt tgattggcaa actcaatgtt
     1081 ccgatagaaa gacttctagc ttttagttgg acatttccgt tttccatcac atacggatca
     1141 aaatataaat agaaatcgat aggaaaacct agtacttcga aagtaccatt gagtagtgct
     1201 tcattttcca aataaaattt atattcaata tcactatctt tttgatactc actaaggaaa
     1261 aagctgatga tttgattgac tttttcttta tttgaattga tcgttaagac aggatctcct
     1321 tgtctttccg taagagctgg tatttctggg agttctggtt cccgattagc aaaaatacgg
     1381 ctgcctaaaa atacggtcga accaagtact accccaagaa ggataaggaa ggcgaatttc
     1441 cagtaatttg ttttctttgt tttgtttgaa gatacgtcag atcttgtttt catgtttatt
     1501 gatcatcctc cttctctaac cattggtttt gtgtctcgat cagctgatct ctcactgcat
     1561 ttgccatcaa ttgataacct aaattatttg gatggaaatg atcttcttca tagagcacat
     1621 tatttttgat gccatttaga ttttctgtcg tgctagttgt ttgatctgtt ccttcttctg
     1681 tgacacctac ttcctgattc aatccttgat aaagcaaatc attgattggg ataaagtagc
     1741 agttcggcat tttttttgtc accgtttctg ttcctttgtt ccaattgtta acaattgtct
     1801 gcatgtcagt gatctcagga aagttcaaat aaaatggatt gtagatgcct aaaatgtaaa
     1861 taggtgcggt tttattgagg gatcgaaatt cttccaatag ttctgtgaca ttttcctgat
     1921 agtttttcag tggcttttta aaggaattga ccgttaaccc gaataaatta ttttggataa
     1981 ctttcatcaa atcattaccg ccaacagtca gtgtgataaa atcagctgtt ttgatattgt
     2041 ttcgaatttc ttcttttgtt tttactcgct ttaagatctg gtcacttcgt tcaccattta
     2101 caccatagtt ttctgtttca accgcggtca aattataacg gtcttttaga tcatttgcga
     2161 caaggggaac gaatcctcct tgcttggttt gatctcctac tccttcagtc agcgaatctc
     2221 cgatagctat gtagtgaagt ttttcttttt tatttggatc ttctctcaca gtagttactg
     2281 gtttcattag tgggccagct ttcggtatag aagcagctaa acctgcatag atgatgatcg
     2341 caattaataa tgggaacaaa acgaggatca aatgtcgttt cgttaatttc attttttcac
     2401 ctcttgtatt cgattggttc taatgttatc aaaaatttta tcaaaaaggt agtctttatg
     2461 aaaaaagaaa acaattattt tcctaatttt ataaagaaaa agagaataag ctgcaagcct
     2521 ttcaggctga gggcttatcc tcttttcgac taatctacat aatacatgat tgcaaacgcg
     2581 tctttaccag tatgcgtagc gataaccgga ttcgtatgca gtacagggat atgcatgtcc
     2641 gggaataatt cttgcaaacc ctctttgaat tcttgcgcca attcttttgc atccgcatgt
     2701 gaaataccga tttgtcgcac atttggcaat tctttcagtt catttttcaa attattaaac
     2761 catttatcga acgttttctt tcctcgcccc ttagttacag ggattaattc agaatgggcg
     2821 aaattcatca ccacgcgtat gttcaaaaca ctagacagca aaccagttgt tcggctgatt
     2881 cttccacctt ttaccaagtt atctagtgtg gatattccga tgtataattt tgtattgtct
     2941 tgtacttttt tgacttcttc aaggattttc tctactgttt taccttcttt tgctaattca
     3001 gctgctcgaa ttacctgaaa agataatcct tggtcagtgg tatcactgtc gataaccgtt
     3061 actttcgttt ttgttagatt actggcttgt cttgctgctt ctactgttcc actcaagcct
     3121 tttgtcatat ggatcgaaat cacttcactg ccgtcttctc ctagctcatc atacatcgag
     3181 atgaattctc caattggtgg ttagcttgtt ttgggcagat tttttgcttt cgccatcatt
     3241 tccataaatc tttcgccgtc tagttggtca tcatcgggat atactgttcc gtcgatcatg
     3301 atggatagcg gcatgacacg aatgcctaaa gcatcgcgta catttttttc catcgtacat
     3361 gaagaatctg ttacgatttt tacatttgtc attattttct ctctctcttt ctttaacaag
     3421 atgccttatt catgttaaac tacatgtaag acaatttcta tttttagtat aacagacaaa
     3481 taatgatttt tcattaatta cacctagtaa aggagccgtt ttcatggaaa aaacgaaatt
     3541 taatcgaacg tatgaaatat taaatgaagt gtttaatgca gtcacccatg gaattgggac
     3601 tggattgagt atcgcgggac tagtggttct tttagtaaaa ggtgcccact tgcattcctc
     3661 cctccatatt gtctcttatg ctatctatgg atcgatgatg attcttttat ttttaacgtc
     3721 tactttgttt cattcattga tttttacaaa agcaaaaaag gtgttccaag tattcgatca
     3781 tagttcgatt tttcttttga ttgcaggaag ttatactcct ttttgtttgc tgagtatccg
     3841 aggatggcta ggttggactt tatttgtatt aatctggttg tttgcgattc ttggcattat
     3901 atataaagcg ttgacgttgc ataaacggga gagagtatca aaagtatcta cgatcatcta
     3961 tatttttatg gggtggctct gtgtcgtggc tgctgttcct ctttatcact ctcttggaat
     4021 gactggtgtt attttgatgg tagcaggcgg gctttcttac acgataggcg cattctttta
     4081 tagcttgaat tctatccgct atatgcatgt tgtttggcat ttgtttgtta tgttaggtgc
     4141 aggttttatg tacttctctg ttttattctt tacttaataa aaaaaataaa gctatggatg
     4201 ggacagctct aaataacagc tagtctatcc atagcttttt tatatcgtca attcgcctgt
     4261 cccgcttttt acaggattct cattacgctg atacgtttca aattgatgcg gataacgatt
     4321 tttttcatcc atttctcctt gactagtatt gatcagtgac caatcagacc agtcaacagg
     4381 tggaaaataa gcatctccat cgaatacatg atggatgact gtacggtata atatcgtaca
     4441 aaatggcaga aattcttgat aaacagctga ccctccagcg atgaaaaaga taccgtctgt
     4501 ttctttttcc agagcaagca cgtcttccac actatgcatg acatgcacgc cttctgcttt
     4561 ataggaagaa tctctcgtca aaacaatcgt ctgacgattc ggcaacggac ggctacccat
     4621 cccttcgaat gtttttcgtc ccatcactag tgtatttgct tctgtcattt gtttgaagaa
     4681 tttcagatca tttggcaaac gccaaggaag acggtcttct tttccaatca atccgttttc
     4741 atcctgtgcc caaattgcag ctaacatttt ctcatccttt ctgattctaa acagcgatag
     4801 gagctttgat cgccggatgt gggtcatatc cttctaattg gatatcttcc atctcaaaat
     4861 caaatactga ctgcttttcc tgattcaaaa ctaaagtcgg aaatgcgcgt atttctcggc
     4921 tcaattgttc tttcatttgt tcgatatgat tgatataaag atgagcatct cctaatgtgt
     4981 gaacaaattc gccaaccgct aaacctgttt catgtgcaat taggtgagtc agcaacgcat
     5041 aacttgcaat gttgaacgga acgcctagaa acacatcccc actgcgttga tacaattggc
     5101 agcttagccg tccctcttga acataaaatt gaaacatcgt atgacaagga ggcaaagcca
     5161 tgctaggaac atcttccgga ttccatgcag aaacgatcaa tcttctagaa tcaggagtct
     5221 ttttgattgc ttcgatcact tgtttgattt gatcgataaa gccaccgtct tttgtttccc
     5281 agtgacgcca ttgatagccg tagatgtgtc caagatcgcc gtatgtttca gcaaaggaat
     5341 catcggttag gatcttctcg caaaaaagct tgctctgctt tttatattct tcattgaatt
     5401 caggatctgt caaacatcta tggccaaaat cagtcatatc tggtccatta tagtctggac
     5461 ttttgatgta gcgttcaaat gcccattcat cccaaatatg gtttttattt tgtaatagat
     5521 agcggatatt cgtatctcct tttaaaaacc aaagcaattc gcttttgatc aatccaaaag
     5581 gtactcgttt cgttgttaac aaaggaaacc ctttttgcag atcaaaccgc atttggtatc
     5641 cgaataagct taatgtgcct gtgcctgttc tgtcttcttt acgatgccct tcttccaaca
     5701 attttcttcc taagtcaaga tacgcttgtt ccatcctttt tcctcctaat cttctaccca
     5761 ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg tttcagatac
     5821 gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca tctcttcttg
     5881 gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt cccattcttt
     5941 ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta tttttggttt
     6001 cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg acatgtttcc
     6061 gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg ctaccttgtc
     6121 caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc taaaataatc
     6181 ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca ataataaaac
     6241 attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc cgcctgaaag
     6301 tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa gtaattctgc
     6361 tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt gtaagtaagc
     6421 aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg caagatgaac
     6481 cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta gaatattaag
     6541 caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc gagattgaat
     6601 caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc catctttgat
     6661 ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat catcttcttg
     6721 gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg cttgctgctt
     6781 ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat aaagccgctt
     6841 gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta agacgtaagc
     6901 ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga ttcggtttgc
     6961 tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac ggtattgttt
     7021 caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg gttcgtctag
     7081 taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt ttttctgacc
     7141 acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt gcaaaataat
     7201 ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt ctgctttagc
     7261 ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca atgcttcctc
     7321 atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt ggagaacagt
     7381 taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat cagttggata
     7441 aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat ttaagagact
     7501 tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt ggaccaaaaa
     7561 agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag ttacttttaa
     7621 ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag tgaacttcag
     7681 gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaac
//
LOCUS       DAHUWM010000132         7631 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHUWM010000132 DAHUWM010000000
VERSION     DAHUWM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.134"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(62..790)
                     /locus_tag="NK788_002006"
     CDS             complement(62..790)
                     /locus_tag="NK788_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6770330.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="NK788_002007"
     CDS             complement(803..2083)
                     /locus_tag="NK788_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6770331.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="NK788_002008"
     CDS             complement(2224..2826)
                     /locus_tag="NK788_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCH6770332.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="NK788_002009"
     CDS             3071..3277
                     /locus_tag="NK788_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770333.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="NK788_002010"
     CDS             3589..4308
                     /locus_tag="NK788_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCH6770334.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="NK788_002011"
     CDS             complement(4389..5072)
                     /locus_tag="NK788_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCH6770335.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="NK788_002012"
     CDS             5290..7476
                     /locus_tag="NK788_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCH6770336.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2735 a         1284 c         1438 g         2174 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggtgt
     7621 gacaagaatt t
//
LOCUS       DAHUWM010000133         7542 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHUWM010000133 DAHUWM010000000
VERSION     DAHUWM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.135"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            16..177
                     /locus_tag="NK788_002013"
     CDS             16..177
                     /locus_tag="NK788_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770337.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            190..693
                     /locus_tag="NK788_002014"
     CDS             190..693
                     /locus_tag="NK788_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCH6770338.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="NK788_002015"
     CDS             753..1142
                     /locus_tag="NK788_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6770339.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..3576
                     /locus_tag="NK788_002016"
     CDS             1129..3576
                     /locus_tag="NK788_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6770340.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            3581..5704
                     /locus_tag="NK788_002017"
     CDS             3581..5704
                     /locus_tag="NK788_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6770341.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            5701..6705
                     /locus_tag="NK788_002018"
     CDS             5701..6705
                     /locus_tag="NK788_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCH6770342.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            6724..>7542
                     /locus_tag="NK788_002019"
     CDS             6724..>7542
                     /locus_tag="NK788_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6770343.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIK"
BASE COUNT         2384 a         1255 c         1720 g         2183 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tggacaagct gtgattgtcg atccaaaagg ggaacggggt
     2641 ggctggaaag aaaccttgcc agaaattgcg gatgaaatca atattctcaa cttaactagt
     2701 aagccaaaaa atcaaggatt acttgatcct tatgtgatta tggagaaaaa gaaggattct
     2761 gaaagtctag caattgatat tctgaccttt ttaacaggaa tttctagtcg agatggggag
     2821 aagtttccag tgttgcgtaa ggccattcgg acggttaccc aaagcaatca acggggatta
     2881 ttacaagtaa ttaatgagct tcgaaaagaa ggctcaccag ttgcggaaaa tatcgcggat
     2941 cacatcgagt cctttaccga ttatgatttt gcccatttgc ttttctcaga tggttcgatt
     3001 acccagtcga ttagtttaga taggcagtta aatatcattc aagtagcgga tttggtttta
     3061 ccagatgcag aaaccacttt tgaagaatat accaccatgg aattgcttag tgtttccatg
     3121 ctaatcgtca tttccacttt tgccttggac tttatccata gtaatcgaga aatcttcaaa
     3181 attgtagact tagatgaggc gtggagtttt cttcaagtag cgcaagggaa aacattatcg
     3241 aataagctcg ttcgtgctgg gcgggctatg aatgccgggg tttactttgt cacgcaaaat
     3301 agtgatgatt tactggatga acgcttgaag aataatatcg ggttgaagtt tgcgtttcgc
     3361 agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggcttgga tcgagaagat
     3421 gaaagcaatc aaaaacgcct acgagatttg gaaaatggcc aatgcttgat gcaggatttg
     3481 tatggtcggg taggcgtggt tcaggtacat ccagtctttg aagaactctt ccatgccttt
     3541 gatacccgtc cacctgttca agagagaatg gagtgagggg atgaaaaaga aactttttcg
     3601 gattgtggga atcgttttta ttagcatact cactctgata gttctattga gtttagttgg
     3661 tacggtagcc gaggcgacag ggctagttga tgatacagtt gaagcaggaa atctatattc
     3721 caaatattca ctgaacaact atcaactaga tttctttgtg gatagctctt gggattggtt
     3781 gccttggaat tggggtgatg gcttagggaa aagcgtgatg tatggtcttt atgccattac
     3841 taatttcatt tggacggtga gtttgtattt gtcgaacgcc acgggttatg tggttcaaga
     3901 ggcttacaaa ttagatttca tttcggatac ggctgaaagt attggaaaga atatccaaac
     3961 attggctggt attactgaaa atggactcca gacctccgga ttttattttg ggtttctatt
     4021 attgatgatt ttagctctgg gggtgtatgt ggcgtatacc ggattattaa aacgagaaac
     4081 cacgaaagca gttcgagccg tgattaattt tgtgatgatt tttctactat cgggttcgtt
     4141 cattgcttat gcgcctacct atatcacaaa aatcaatgac tttagctcag atgttagtga
     4201 agcggccctt acccttggaa ccgagatcgt tgtacccaat tcagaaagcc aaggaaaaga
     4261 tagtgtggat ttgattcggg atagtttgtt ttcgattcaa gtccaacaac cttggttact
     4321 actgcaattt gatgattcca acatagaaga aattggtgaa gaccgggtca acaagattct
     4381 atctgtgagt ccagatgaaa acaaaggcaa agatcgggaa gaagcggtca aggcagagat
     4441 tgaagacaat gacaatgcga atctcagtat taccaagacc atgaaccgtt tagggaccgt
     4501 ggtctttttg gtattgttca atattggcat ttccttcttt gtttttcttc taacagggat
     4561 tatgttgttc tcgcaaattc tcttcattat ctttgcgatg tttctgccaa ttagtttctt
     4621 attgtccatg ttgccgactt atgaaagttt aggaaagaag gcgattattc gtttattcaa
     4681 tacgattatg atgcgagcgg gtgttacttt ggtgattacg actgcattta gcatctccac
     4741 gatgtttttc aatatttcgg caacctatcc atttttcatg gttgcttttc tgcaaattgt
     4801 gacctttgcc ggtatctatt tcaaattggg cgacattatg agtatgttca acctccagag
     4861 taatgatagc cagtcaatgg gaagacgcgt catgcgaaaa ccgcaaatgt taatgaatcg
     4921 aaaattgcgg caactcaatc gaaatgtggg acgagctttg gcttttggtg gtggtgcagc
     4981 agtcggaaat aagttggcaa aggaacaatc aaaatccaaa tttaagccat ccggttcttc
     5041 tctacgaaag aattcacgat taccgaacga tcacgaaata ctatctgact taactaagga
     5101 gaatccagtt tcaaataata agaaacaatc ccgaatgaat ttggctggga gaaaaatcgg
     5161 caaagttttg gatacccaag ctttggtgaa agataagact aaacaagtaa aggatcaggt
     5221 aaggaatacg ccaacgaatt tgaaatacaa tctccataaa ggaatcgaaa agtcgaaaaa
     5281 agcaccagaa gaattcaagc gcggactgtt ccaagaaaaa gcggatcgag tggatttgcg
     5341 agaaaaacaa cgacaacgaa gaaatgcgaa aatggacgaa aaacgcaaga tactgagtga
     5401 agcaacaaat cgtcagggaa aaagaagggc gaatattcca atagataaag cagctcaacc
     5461 tcagaaagat atgatgctga aaaaagaagc accaaagcgg attgtgagag caaatctgaa
     5521 tcccgaaatc aaacgaaaac taactagtca agaaatcatt tcaaaaggga aaagccaatc
     5581 agcaagtaag aaaaattctt tccaacaagt gaaacagagg tccactctgc caaagcagac
     5641 caatcaaaaa gtacaaaaaa gaatgaacca tcgtccaaaa tcaaggtcag gtgagaaaaa
     5701 atgaacttga agaaagtggg tgttatttca ttaacttttc tgaccatctc ttttatgggt
     5761 attttgatgt gtgtgggttt gttgtttggt gaagacagtg agggaaatgg tagttctggc
     5821 acttctcctg gcggaactag tgtctcagaa gaagtattga aacaccgtgc aatggtggaa
     5881 aagtatgcca aagaatcgga tataacagag tatattccaa ttcttttggc gattattcaa
     5941 gtggaatctg gtggcacaat ggaagacgtt atgcaaagtt ctgagtcagc cggattacca
     6001 cctaattcat taagtaccga ggcatccatc aaacaaggct gtctctattt tgcgtctttg
     6061 gttaagcgtt ccaaagaact agggtgtgat caagatagta ttattcaggc ctataactat
     6121 ggtggcgggt atctagatca tgtcgggaaa aacgggaaga agcatagttt tgctttagct
     6181 gaatcttttt caaaggaaaa atcaggtggt caaaaagtcg attaccccaa cccaatcgcg
     6241 attaaggaaa atggtgggtg gcggtataat tatggcaata tgttttactg tttattagtg
     6301 aagcaatacc taactacaac tcagtttgat gacaaaatag tccaagaaat ttttgatgag
     6361 gcatttaagt atgaaggaac ggcctatgtg tttggtggct caagtccaga aacggggttt
     6421 gattgtagtg gcttgaccca atggtgttat gccaaagttg acctgaaact tcctcgggtg
     6481 gctcaggatc aatatgatgc catgactcat attgatctaa aagatgccaa accaggtgat
     6541 ttagttttct ttcattcaac ctatgatgcc ggtacttatg tgacccatgt gggcatttac
     6601 gcaggggaga atcgcatgtt tcatgcagga gatccagtgg ggtggacgaa tctaacggaa
     6661 aactattggc ggcagcatct tatcggagct ggccgataca aataagagaa aagaggaaaa
     6721 agaatgaaga taaaaattga acggaatcaa aaggaaaaga gcccaaaaaa gaaaaaagaa
     6781 cgagttgtat ccgtgggtaa gcatcgaaaa atggtgcttg ccctttggct tttattgtct
     6841 tgtagtcttg cttttggcgt ttataaaaat ttcaccgcca ttgatcaaca tacaactcat
     6901 gaaaaagtgg ttataaaaga gaaggtagtg aatacttctg gcgttgaaag ttttacagag
     6961 gattttgcga aagaatattt ttcatggaaa aataacaaag aagcaattga aaagagaatg
     7021 agtaatctcg aacagtacct tacagaaaac ggtctcgctt taatccaaga tatggttcga
     7081 gcagatattc ccacgagttc cgaagttcaa tctgtaaaaa ttctagatgt tgaaaaacac
     7141 tcggaagaat ttgtcgtttc atttttagtg gagcaacata ttacagaggg aaaaaagacg
     7201 caatcaattt cttctgcata tcgagtgaca atctttgaag atgaaaacag gaatcattta
     7261 gtaactagtt tgccaacaat gattggaaag ccagacaagg caaaatatga agcaaaacaa
     7321 gtagaaaacg attcagaaat tgatgccaaa acaacggaag aaatcaccga ttttttagaa
     7381 acatttttta aactttatcc atcagcttct gaaaaagaat tggagtatta tgttgaaaat
     7441 ggtgcgatgc gaccaatcaa tattgactta aagttttcgg agttagagaa tccagtttat
     7501 ctacaaaacg gaaaaaattt tctagtgagt acaaagataa aa
//
LOCUS       DAHUWM010000134         7450 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHUWM010000134 DAHUWM010000000
VERSION     DAHUWM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.136"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            146..1537
                     /locus_tag="NK788_002020"
     CDS             146..1537
                     /locus_tag="NK788_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770344.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1610..2257)
                     /locus_tag="NK788_002021"
     CDS             complement(1610..2257)
                     /locus_tag="NK788_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCH6770345.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2265..2951)
                     /locus_tag="NK788_002022"
     CDS             complement(2265..2951)
                     /locus_tag="NK788_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770346.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2957..4030)
                     /locus_tag="NK788_002023"
     CDS             complement(2957..4030)
                     /locus_tag="NK788_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770347.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4252..4926
                     /locus_tag="NK788_002024"
     CDS             4252..4926
                     /locus_tag="NK788_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6770348.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4931..6271
                     /locus_tag="NK788_002025"
     CDS             4931..6271
                     /locus_tag="NK788_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6770349.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6420..7406)
                     /locus_tag="NK788_002026"
     CDS             complement(6420..7406)
                     /locus_tag="NK788_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCH6770350.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2309 a         1383 c         1316 g         2442 t
ORIGIN      
        1 ggcgctaaag ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc
       61 cttcgattaa tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag
      121 gttgttggaa ggaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga
      181 caagtggatg actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt
      241 aaggtatatc gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca
      301 aaaattgggg tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga
      361 aatgtcgcaa agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa
      421 aacgattgag tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt
      481 gaaacaatgc aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt
      541 tcagttaact agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt
      601 aagttactac ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat
      661 tatggtcaag aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc
      721 tttctttatt atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga
      781 atggaaagct ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat
      841 cttgaaaaat ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt
      901 tgtcaatacg attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat
      961 acgaaaaaat atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga
     1021 atatgttgtt caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac
     1081 gactcatttg acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg
     1141 tctatatgct ggatttcgtg cagaacttca attttattcc actctactga atcagaatct
     1201 gatttattat ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca
     1261 ttatacttct gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata
     1321 tattgaagaa tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc
     1381 tattactgaa gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa
     1441 tcaacccatt ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc
     1501 aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat
     1561 aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc
     1621 cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat
     1681 tatttgcaat gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac
     1741 taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt
     1801 tttcgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc
     1861 gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc
     1921 actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta
     1981 agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca
     2041 aatcttgaaa atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg
     2101 agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc
     2161 tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt
     2221 tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc
     2281 tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata
     2341 cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt
     2401 aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag
     2461 atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca
     2521 gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa
     2581 tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc
     2641 tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag
     2701 cgtaaatctg ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt
     2761 tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa
     2821 ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc
     2881 aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat
     2941 atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac
     3001 gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc
     3061 attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag
     3121 caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag
     3181 atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct
     3241 tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa
     3301 agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga
     3361 atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg
     3421 aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc
     3481 catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat
     3541 aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg
     3601 tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg
     3661 ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga
     3721 aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg
     3781 ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata
     3841 cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt
     3901 taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca
     3961 agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag
     4021 ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg
     4081 aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat
     4141 tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt
     4201 atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa
     4261 ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca
     4321 atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga
     4381 gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta
     4441 gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt
     4501 ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc
     4561 tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt
     4621 gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg
     4681 tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta
     4741 caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat
     4801 tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga
     4861 agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg
     4921 gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa
     4981 tgtagcagca tttgctttta tcttttttct tttgggattc attactttac agatattaaa
     5041 ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac
     5101 agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac
     5161 ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa
     5221 cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg
     5281 gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa
     5341 tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa
     5401 agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat
     5461 caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat
     5521 tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc
     5581 ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac
     5641 gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga
     5701 atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga
     5761 tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa
     5821 tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg
     5881 aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat
     5941 ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa
     6001 gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg
     6061 cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca
     6121 atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat
     6181 cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa
     6241 agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa
     6301 aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg
     6361 gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct
     6421 tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct
     6481 tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca
     6541 acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata
     6601 gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt
     6661 ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg
     6721 accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat
     6781 ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca
     6841 gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt
     6901 gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga
     6961 tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct
     7021 tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca
     7081 aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc
     7141 gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg
     7201 tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga caatccgtca
     7261 aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac
     7321 ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg
     7381 atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg
     7441 tatattgtta
//
LOCUS       DAHUWM010000135         7349 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHUWM010000135 DAHUWM010000000
VERSION     DAHUWM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.137"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..682
                     /locus_tag="NK788_002027"
     CDS             <1..682
                     /locus_tag="NK788_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCH6770351.1"
                     /translation="YNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSW
                     VIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSII
                     YFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLF
                     YNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARR
                     FDSDSALF"
     gene            694..1614
                     /locus_tag="NK788_002028"
     CDS             694..1614
                     /locus_tag="NK788_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCH6770352.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1655..3112
                     /locus_tag="NK788_002029"
     CDS             1655..3112
                     /locus_tag="NK788_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6770353.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3457..4071
                     /locus_tag="NK788_002030"
     CDS             3457..4071
                     /locus_tag="NK788_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCH6770354.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4064..5788
                     /locus_tag="NK788_002031"
     CDS             4064..5788
                     /locus_tag="NK788_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCH6770355.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5785..7236
                     /locus_tag="NK788_002032"
     CDS             5785..7236
                     /locus_tag="NK788_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6770356.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2508 a         1222 c         1469 g         2150 t
ORIGIN      
        1 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
       61 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
      121 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
      181 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
      241 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
      301 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
      361 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
      421 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
      481 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
      541 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
      601 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
      661 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
      721 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
      781 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
      841 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
      901 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
      961 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     1021 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc
     1081 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     1141 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     1201 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     1261 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     1321 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     1381 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     1441 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     1501 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     1561 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     1621 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     1681 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     1741 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     1801 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     1861 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     1921 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     1981 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     2041 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     2101 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     2161 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     2221 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     2281 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     2341 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     2401 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     2461 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     2521 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     2581 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     2641 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     2701 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     2761 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     2821 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     2881 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     2941 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     3001 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     3061 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     3121 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     3181 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     3241 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     3301 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     3361 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     3421 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     3481 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     3541 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     3601 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     3661 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     3721 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     3781 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     3841 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     3901 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     3961 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     4021 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     4081 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     4141 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     4201 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     4261 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     4321 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     4381 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
     4441 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
     4501 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
     4561 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
     4621 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
     4681 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
     4741 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
     4801 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
     4861 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
     4921 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
     4981 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
     5041 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaggg aactttcaat
     5101 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
     5161 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
     5221 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
     5281 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
     5341 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
     5401 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
     5461 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
     5521 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
     5581 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
     5641 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
     5701 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
     5761 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
     5821 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
     5881 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
     5941 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
     6001 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
     6061 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
     6121 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
     6181 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
     6241 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
     6301 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
     6361 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
     6421 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
     6481 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
     6541 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
     6601 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
     6661 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
     6721 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
     6781 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
     6841 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
     6901 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
     6961 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
     7021 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
     7081 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
     7141 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
     7201 cctaaagagt accgagaaaa cagtggagaa tcataaaaga aaaagccaat gtaatgaaat
     7261 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
     7321 cagctttttc atgacctctc gataaacgg
//
LOCUS       DAHUWM010000136         7323 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHUWM010000136 DAHUWM010000000
VERSION     DAHUWM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.138"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(119..1321)
                     /locus_tag="NK788_002033"
     CDS             complement(119..1321)
                     /locus_tag="NK788_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6770357.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1438..1953
                     /locus_tag="NK788_002034"
     CDS             1438..1953
                     /locus_tag="NK788_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6770358.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2152..2598)
                     /locus_tag="NK788_002035"
     CDS             complement(2152..2598)
                     /locus_tag="NK788_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCH6770359.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2645..4123)
                     /gene="msr(C)"
                     /locus_tag="NK788_002036"
     CDS             complement(2645..4123)
                     /gene="msr(C)"
                     /locus_tag="NK788_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCH6770360.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4477..5106)
                     /locus_tag="NK788_002037"
     CDS             complement(4477..5106)
                     /locus_tag="NK788_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCH6770361.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5090..5554)
                     /locus_tag="NK788_002038"
     CDS             complement(5090..5554)
                     /locus_tag="NK788_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6770362.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(6041..7309)
                     /locus_tag="NK788_002039"
     CDS             complement(6041..7309)
                     /locus_tag="NK788_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCH6770363.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT         2219 a         1525 c         1267 g         2312 t
ORIGIN      
        1 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt
       61 tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct ctgatttctt
      121 attcttcttc atttgtagca aaataattac ggtgataaat gatacaacat acactgataa
      181 gaacaatggc acctgcaaaa taaaacgcat agctttcacc aatatgttca ccaatcacac
      241 ctgcaataaa tggtgctaaa gctccaccga aaaagcggac aaaactatag ctggacgaag
      301 caacgtttcg ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt gtattgataa
      361 ttccttggaa aaatcctgct aacacaatgc tgatcggtac aaggactggc atagaagttc
      421 cagctcctaa taacagcaaa caaataatga ataagacaaa tgctgctaaa attgtccgat
      481 aagtcgtaaa cttccgctca agagccggtg caacgaaaat cgaagcaata gccagaagca
      541 caccccagcc aaaaaataca aaaccgactt ctaattcaga atagccaatc aataaaaatg
      601 gcgcatacgc aagtaaagta aagaatccga aattatataa caatgcgatt agtccaactg
      661 aacgaagttt atgattgctt aacgctttga caccttccca aaggcctact tttctctttg
      721 gtttctcagt ttcaggtaaa agtaccgcta gggctataaa accgcaaaac atcaaaaatg
      781 ctaccccaaa aaatggataa cgccatgaac cactgcccaa gaccccaccg actagagggc
      841 caacagccat tccgcagcca agtgctgctt catacatcat gattgctttt tctgtgttac
      901 caactaaaat actgacgatc gttgctaaag cagtagagac aaataaagca ttcccgacac
      961 cccagccggc acgtaaccca atcagcattc cgatattccc agataatccg ccaagaccag
     1021 cgaatacaac gatgataaac aatcctgtca tcaatgtttt tttcaaaccg atccgactag
     1081 ataagaaacc tgtaaatagc atcaccacac cagtgactaa catgtaacta gtaaataaca
     1141 gggtcgtttc agctggcgta gcgtttaact gtgcagcaat cgtttttaag attgggtcta
     1201 ctagacccac tcccataaaa gcgatcatac aggtagctgc tacaactagc gctgaacttt
     1261 tcccctttgt ttcttcattt gtttgttctt gtatatctat ttgattctcc aatatttcca
     1321 tccctttctc tctattagca tcatacatga tttattttac atgtcaatat gcacatgtaa
     1381 aacgtggaaa ctttactgtg atataataaa gaaaataaga cgagaggtag gatttttatg
     1441 aatactttag cctatatatt attatgcgtg cttttatcca aaccacgttc tgggtatgaa
     1501 ttgaagcagc tgatcacaat tttttgggaa gcccatcaca gtcagatcta tacaacgctt
     1561 gccaagttag accagcaggg ttatgtgacc gttttggaca aagacgagca ctctcaaaaa
     1621 aagatctacc acttgactga tgaaggacgt gctcttgtag aggaatggat caaagaggaa
     1681 actcccgctc ctacgcaaaa agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc
     1741 gataaaacaa cagctactgg actgctattc actagagaac aacagctgaa caaaatcatg
     1801 caaaaaaatc aaaaaaaatt gaatgacctc tcgctagaca agaaacaaga atttggacgt
     1861 tatgttgtga tccagcgcag aattatgctt tgtcagcagg aactccaatg gtgtcagcaa
     1921 gtaagagaac aaatgcagca gttttttgag taagggataa acagaacctt tctagaaata
     1981 tctattgaag agcaaattat cttcaataaa agagcaatac agttggaaaa gaaaaagcga
     2041 tccgaacgac aggccaaaac ctatcgttcg gatcactatt tttctagaac tgcattcaag
     2101 agactatgac aaaaagcatg ccaaggcctt ttttcaaata acagtgttcc atcattcatt
     2161 actttgaatt gcttttagca cacttcttga aagcgggcca acgatgatca actgcaacgg
     2221 taaagcggca atcacattca ttttccaagc attcatataa gcagagaata attgatcagg
     2281 tattccttgt tccatgatca caccgaataa agacatacat gtcaccattc ctaaaaccat
     2341 caagcatgaa atagttaaga tcatttggac cttgctttct ggatttactg gtagtttaaa
     2401 ggcaattttt ttcgccagca caccgatgat gaagacatcc aaaatgaaag ccaccacgaa
     2461 tccaggcact agaccaatca acaaacttga taaatgaaga tcttgatgca gccataaatt
     2521 atacgcgctc atccccaaga ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc
     2581 ttttttattt gtaggcaatc tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc
     2641 acatttaaaa atttctcgta agtacttttt ttattttctg cccttttatt tcataaactt
     2701 catcagccgt acgctcgaca aagtaagaat catgagaagt aaacaaaatc gttcccggat
     2761 atatttgcat tagcttctct aaagcttcga tcgttgccat atcaataaaa ttagtcggtt
     2821 catctaacag caacacatta cttggcttag taaaaagcaa agcaatcgtc agacgggtcg
     2881 cttctccccc acttaatgta cataacggtt tcgtcaaagt ttcctgagca aaccctaagt
     2941 gattcagcaa actacggaca agcgattctg aaaactctgt ttgtctcatg acaaaacgaa
     3001 tgatggattc ttcagatgtc atttgataac ccttttgctg atatacttga aagcttactt
     3061 tcggagagag aaggattcct tctccttgtt tgcggatatg ttctaataag gttgtcttac
     3121 ctgaaccatt ttcaccgaca atcgcgattt ttttacctaa agaaaactga aaatcaccat
     3181 ttaccagtaa tgttctccca cttctttcca attggacatt ttgtcccatg atgggatagc
     3241 ggctgtggag ttcaagagat tttggtactg gaaaacggat ttgtttcatc tgctgcggct
     3301 tggttgtttc tcctattttt tggagtcttc tctctaatgt tttcgcattc ttttggatgg
     3361 ctttttgtac actgtctttt tgtttagagg aagacaaccg atccagtctg atttgttgct
     3421 ttttcttttt actggacact ttttccaact tttccgcttg ttttttcttt tcttggatcg
     3481 atttttccaa tcgttttttc tctttcatga tcgactctgc ttctcgtagc tgagtctttt
     3541 tctccaattc cttttgagtg agataggcac tataattccc cgaaaactct cggatttctc
     3601 cgtcttttac ttcccagatt ttcgatgcta gctcatctag aaaatagcga tcatgactaa
     3661 caaaaaccag cgtaccataa taataacgaa gctcttcaat cagtcgatca atactttttc
     3721 tatctagatg ggttgttggc tcgtctagaa gcatcccttg ttcataagaa gaaagaagac
     3781 gtatcaactc gatctttcgt tcttcccctc cgctccattt ccgctctgaa gtttcttcca
     3841 gtcttaactg gaaatgactg gctaattcca aatttaggtc ctttgcttca gcgagatagg
     3901 ttaattgagg caggtagttg aaggtgatct ctgtttggat tttcccatga tcgggaaaaa
     3961 gttcaccggc tattagtttg agcaaagtcg atttaccgac accattttct cctatgatcc
     4021 ctatacgatc ttgctgatag acacgaagag aatcaataga tagttctagc tggttgccaa
     4081 agctgacttg tagatttgtt atatttactg ctaaattttc catatgtttt acactcctat
     4141 ttcatgtaaa acaacaatta tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa
     4201 actgcatcac atcttttcct actctgaacg aaataactat gactaaatca ccaaatctgt
     4261 catagctttc acctattcta ctgtcgttcg aaaaaatgga catactccat ccatgaaatt
     4321 gttgtttata catgtctttt gaatttattt agttgttatt caaaagttcg aaacgtaatt
     4381 tcatcgatgc agtcattttt ccgacctcct tttttattag gttaccacac acgattcaaa
     4441 aaaagcaata gctactgaat agtaactgaa aaccttttat tcgaacatag tgatcttttc
     4501 tggtgctaaa cgaacagact cgtaaccttc ttctaccgct tttcctattg ataaaataat
     4561 aactggaaca taacggtctt tatccaaacc aaatgcctct gccaactgat ctgcttcgaa
     4621 tccaccaatt ggatttgttt catagccatg atcacgcgca actaacatga attgcatagc
     4681 cgctaagcta gaatcgactt tgactacatc gttcatttct tctcttgaaa aactcttgta
     4741 ataaggaatg atcgcagcta attgttgatc tctgacttct tttggcatct tccctgattc
     4801 gtaagcttgg ttgtagattt cttcgccata ttcgtaacat tgcatatcgc caaaaatcat
     4861 gaccattgca gaagaagtat cattttgtct cgtattgaaa cggatcaaag gacgcaagat
     4921 ttcttttgct tcctcgcttt cagcgaccac aaagcgccat ggtttcatat tgacagaaga
     4981 aggtgcagtc gtcgcttctt tgatcatttc cagcatttct tcgtgtggga ttttgtatgt
     5041 ttcatcatat acgcgaacgg atcttctacc agtcataatt gttgaaaaat catttttttg
     5101 ttccattatt attaacctct ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt
     5161 tatctaaaag tgttagaaat tgtttcattt ctacatcgct gaaagccggg gatagcacgg
     5221 aacctgtatc tttatggtcc atttcacaat gcttgatttt cttctgggct ttttctgtta
     5281 gcgctacata cacttcccgg ttgttctgag gatttctctg acgcgaaaca tagccatttg
     5341 cttccaatac cttcagatga cgagtaactg ctccttgatc aatctgcaac ttttcttgca
     5401 aactgctttg tagcaatgga ttttcttctt tcagtctcat catgatctcg tatctagtaa
     5461 gacttatacc gatattttct tcaaagatct ggacgatttt ttgatcggct aatttgattt
     5521 gatataaaag gtgactaatc tcttctaaag acataatttc ctcctttcta ataattgact
     5581 cgtcaacgat tgacttgtca ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg
     5641 tgtaataaaa aaaacgctct caatagaaaa gtatagagaa aagaaatttt ttgtagaatg
     5701 aacggaaaaa atgataaaga aagcacaacg ctaaaaccaa gattattgat tcttatcaaa
     5761 ctatagaaga tagctcttct gcagtagata attttacacc gccaaaaatt tccgaaatcg
     5821 ccttttgcaa gaatcccgtc gaatattaga aattggatca gtatgcttaa acagtaataa
     5881 aaaagttatg acaagatgtt gtcacaacct tttttgagta aacggtgttc aaaaacttgc
     5941 atctgcggta ataagttcaa ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt
     6001 cttactactt gatgcagggc agctttttca ctacctcttt ttaataacca taaaatccta
     6061 ttccttcata tctccagccc atcggtgtta gccaatctct ttccttagca ctcaaggtat
     6121 agtgatgggt accagttttt tcatttggat taaatagtcg ataaattggc ttggaaccac
     6181 cagaataaaa acttagacct tcataacgcc aaccgatttt tgttaaagag ttgatctcag
     6241 attgtgccat tgtgtaatga tggtctccac tgtaaggatt gtataaacgg tagacaggtt
     6301 gtccgctgtt tggtgcttgc caagcaatcc cttcatattc ccagcctaca gttggcagat
     6361 aatttttttc tccggcattc aatgtatata aatgttctcc tgtatgacgg ttatacagac
     6421 gatacatcgg agaagttgaa gttggtgctg tatcatactg tgtcagatta taggtttcga
     6481 ttacattgtt caatgtagaa ccataattag gcgcagtggc ataacgacct gttagccaag
     6541 ccgtagcatc tttgtaagag ttcgtattgc ttttccatgc accggaatag tagtaaacgc
     6601 ctggctgaaa agaagtcgtc ttcaataccg ttacgttatc ttgcaatgat tctttgtacg
     6661 aaggatattt tctaaaagcg gcattcaccg taatccattg cccattcaca tattccgaag
     6721 taggcatcgt aacagattgg ccattatagc ttcctttaat cccaaataga ttgtaattag
     6781 gggcagaagc caaagtactg ttcccccagc cactttcaac gatggcttga gcaatcatga
     6841 ctgatgcata taagtcattg gcacttgcca aactttgtgc agttgaggca atactatcaa
     6901 tgaacgcttg ttgagaatta gcagatgagc gagcataaaa agcatagcca gtatagtttt
     6961 cgaattcaga caaatctgct tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt
     7021 tcgtgatttc ttctgcattc tctggattgt tctgagcttc tgctgatgta ctgtcttcgg
     7081 tttgtgtttg ttctgttgtt tcagttgttg gttcttcggt tgaaattgta ttttctgaga
     7141 cagtagctga ctgcaaagta ctttctgtat cgttttcaat tcctgtttct gaagaactcg
     7201 atgtctgagt tgcgacagtt tctgtcgatt cttcagctag cactgttgtg ttggcaactg
     7261 ctagatttcc taaaagaata acgctaaata ataaattctt tgctttcatc ttttttctcc
     7321 ttt
//
LOCUS       DAHUWM010000137         7257 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHUWM010000137 DAHUWM010000000
VERSION     DAHUWM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.139"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            109..1530
                     /gene="arcD"
                     /locus_tag="NK788_002040"
     CDS             109..1530
                     /gene="arcD"
                     /locus_tag="NK788_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCH6770364.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1544..2662
                     /locus_tag="NK788_002041"
     CDS             1544..2662
                     /locus_tag="NK788_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCH6770365.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2722..4014)
                     /locus_tag="NK788_002042"
     CDS             complement(2722..4014)
                     /locus_tag="NK788_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCH6770366.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            4146..4724
                     /locus_tag="NK788_002043"
     CDS             4146..4724
                     /locus_tag="NK788_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6770367.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            4853..5230
                     /locus_tag="NK788_002044"
     CDS             4853..5230
                     /locus_tag="NK788_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCH6770368.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5163..7235
                     /locus_tag="NK788_002045"
     CDS             5163..7235
                     /locus_tag="NK788_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCH6770369.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         2424 a         1331 c         1564 g         1938 t
ORIGIN      
        1 atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa aatagaaggg
       61 aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat ggaaaagaaa
      121 tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc aatcggaagc
      181 ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc agcgatcctt
      241 tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa tcatttagga
      301 gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc atttggtaaa
      361 ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg aaatgttgct
      421 tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa aggtggacaa
      481 aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt attagtaaac
      541 aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa attagtgcca
      601 ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt cactgctgat
      661 ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg aacgatctgg
      721 ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat cgaaggcgca
      781 agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc gatcattggt
      841 ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg cgtcttatct
      901 caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa aggaatcgtg
      961 ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat tggttcttgg
     1021 ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga cggtctgttg
     1081 ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt agttgttact
     1141 gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc ctatcaattt
     1201 gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg gatctatcaa
     1261 gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg gattgctgta
     1321 gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt atgtagtatt
     1381 gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa acatcaaatt
     1441 acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct ttcaatcggg
     1501 ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa tcgcaaattt
     1561 cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata ttgcacaaag
     1621 ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta gcgtaagtaa
     1681 atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag gatcagatgt
     1741 tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca tcttacaaac
     1801 gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag caggagacca
     1861 tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat ctttaggtgc
     1921 cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt tagaacagct
     1981 agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa acaatcctac
     2041 aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc gtacagtaga
     2101 tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg aattcttgtc
     2161 gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa cctactccat
     2221 tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag tttttcgaaa
     2281 gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg ctgtacatgc
     2341 attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag agaaccttgt
     2401 gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc ccaattacgt
     2461 ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt gtatcaattt
     2521 attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac cgaagcatgc
     2581 ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc tcttatctga
     2641 gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga caactgttca
     2701 agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg aagctatgga
     2761 gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg tattgtgtcg
     2821 tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc cacgataacc
     2881 aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag ttgcttggaa
     2941 agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt ttgtgatcaa
     3001 tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac gtaaggcagc
     3061 taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg caaaacccaa
     3121 accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa atcttggagc
     3181 ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca acgtaacatt
     3241 cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa tggaattgtt
     3301 ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg gatatttcaa
     3361 gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga caaatttttc
     3421 atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg taatgaaaaa
     3481 ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc caagtaatgt
     3541 tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt cttttggcgt
     3601 cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc cttgaagcag
     3661 acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa tgacgattgc
     3721 cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg ctaattttcc
     3781 ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca atccgctcca
     3841 aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg acataaaagg
     3901 catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga caaacaagtt
     3961 ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag ccaattagac
     4021 tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat atgtagttta
     4081 tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag tgaaggattt
     4141 attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag aaaatcatcg
     4201 actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt cagcatttag
     4261 ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat attcgtgatg
     4321 tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg cgacgggctc
     4381 ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat cgatcctttg
     4441 ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc aatgaagctg
     4501 ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt gaggcttttc
     4561 aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg atccacggtt
     4621 atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat ccagaagaca
     4681 gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc agattcattt
     4741 gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc ttgcaacttt
     4801 cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga gtatgtatca
     4861 agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact gggaagaaga
     4921 tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt acgaaaaaca
     4981 atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact ttctcagcgc
     5041 cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt tacagcaata
     5101 caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca gaaaggaatt
     5161 ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta agcaaggtgc
     5221 ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc gttacttggg
     5281 cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa tggcagagct
     5341 ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca cttcctaaaa
     5401 caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc tcagaagcag
     5461 tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc ttagagtatg
     5521 tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat aaagcaatca
     5581 aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat gaatctgctc
     5641 ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc ttaactgtga
     5701 agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca ttagtgagag
     5761 accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc acgttgcaag
     5821 ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa acacaagaag
     5881 aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca gaagtccaag
     5941 aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc caaagagaag
     6001 ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa agtttgtatg
     6061 agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca aatgacatga
     6121 aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag gtcaagcagt
     6181 atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt aaagtgaccg
     6241 atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta agtaatgaag
     6301 agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct caaccccata
     6361 aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt tttcggcaaa
     6421 atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa acagtcaaat
     6481 gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta tctgcaccgc
     6541 ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt ctgggaaccc
     6601 cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg gaacgtaccc
     6661 ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta aatccctctt
     6721 tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt gctgcaggaa
     6781 agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc ttggtgaatg
     6841 agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag aaatgcccag
     6901 aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat gtctgcacca
     6961 attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat cgatgcccgc
     7021 aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc cgttgtaaat
     7081 acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa atgaccaaac
     7141 atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa gaaagcccat
     7201 tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag gttgtga
//
LOCUS       DAHUWM010000138         7200 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHUWM010000138 DAHUWM010000000
VERSION     DAHUWM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.140"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            340..813
                     /locus_tag="NK788_002046"
     CDS             340..813
                     /locus_tag="NK788_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCH6770370.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            810..2942
                     /gene="feoB"
                     /locus_tag="NK788_002047"
     CDS             810..2942
                     /gene="feoB"
                     /locus_tag="NK788_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCH6770371.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2944..3078
                     /locus_tag="NK788_002048"
     CDS             2944..3078
                     /locus_tag="NK788_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCH6770372.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3172..3816
                     /locus_tag="NK788_002049"
     CDS             3172..3816
                     /locus_tag="NK788_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6770373.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4003..5196
                     /locus_tag="NK788_002050"
     CDS             4003..5196
                     /locus_tag="NK788_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCH6770374.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5189..6916
                     /locus_tag="NK788_002051"
     CDS             5189..6916
                     /locus_tag="NK788_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCH6770375.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2345 a         1251 c         1595 g         2009 t
ORIGIN      
        1 aaaaagtcaa acaacaaaca ctacatattg tgttgtggaa actgtggaat actaagtttt
       61 gtgtttttga aaaaagagct gaaaacaagt atcgtgtggg aaaatagcaa atttaataga
      121 gaaaaaattg tggaaaacta tgaagaaata tttttttctc atttttatct atcaatacta
      181 aagattcgct cataatatca caagtataat tgacattctc aattaccgag agtattctat
      241 gtgagaatga ttctcattta tttgcgtaac acgtaagaaa attagaacat ataatgaatg
      301 attttaatac atgatttatt tcacaagaga ggaatcaata tgcagacctt agcgcaggca
      361 aagcagaatt tcgtttatca agtcgaacga atcgaaacag aagaaaaaac aaaacgccat
      421 ttgcaaaata tcgggatcgt tgccggaagt aaaatcgtcg tcgtcaattt atcaggcaac
      481 aatgccattt tactggtaaa aagtcatcga gtggcagtaa gtcgggaaat actaggtcaa
      541 atctttgtca ataaaaagac tgcttcctct gaaagctgga cttctcttga tcaattaaaa
      601 gcaggagaga ctgggatcgt cgtagcaatc cacggacaag gcgcagtgaa gcgacggtta
      661 atggatatgg gattgacgcg aaatgtaaaa atagttatcc gaaaactagc ccctctggga
      721 gatccaatcg aactgacagt gcgagggtat gaactgacgt tacgaaagtc agaggccgaa
      781 ctaatcttga tacaaaggga ggaaaaagaa tgaatatcgc gttagtcgga aatccaaaca
      841 gcgggaaaac tagcttgttc aatctcttga ctggttcgaa tcaatatgta gggaattggc
      901 cgggcgtaac agtagaaaaa aaagaaggat cgttgaaaaa aaatccgaac attcatattc
      961 aggacctacc cggcatctat tctttgtctc cttatacacc agaagaagtg attgcaagag
     1021 attatttggt tcaggaacag cctgaactga tcatcaacat tattgatgct accaatttag
     1081 aacgaaatct ttatttaact acgcagttat tggatttaga cattcctatg atcattggtc
     1141 tgaatatgat ggatgtcatc aaaaaagaag gaaaaaaaat caatctagaa aagctttcct
     1201 atggtcttgg tgtaccagtt ttaccaatca gcgtattgaa aaagcaagga atcgaaaaac
     1261 taatcgaaaa gattcaggag tctgcaaaaa agcggccttc tattcctgtt ccctgtacat
     1321 atgatccacg tttagaagca gctttacatg aaattgaaga tgtactagga gaacaaagcg
     1381 gcaagaaacg ctggtatgca atgaaatatt ttgaacgaga tcagaaggta agagaggatt
     1441 acgagatagc agtttctcag caaaaagaga tcgagcaact gatccagtta acagagaaac
     1501 tattggacga cgatagtgag acgattctgg tcaatgaacg ttatgaattt attacccaac
     1561 tatgtacact ctgtgttgtg tcaaatgatt ctttccaatt atcgatgagt gacaaaatcg
     1621 atcagattgc aacgaatcgt tggttagcct tgccgatctt cgcatttgtg atgtggctga
     1681 tctattatct ggctattcaa acagtaggaa caatggggac agattggatc aatgacacat
     1741 tgtttggtac atggttacca gaacatgttg gcagattgtt agctgaatgg caagtagcgg
     1801 gatggatgca ggagctgatc ttgaacggca tcatcgcagg cgtgggagca gtgttaggat
     1861 ttctaccgca attgattata ttgtttttat gcttgagctt tctggaagac tgcggatata
     1921 tggcgaggat cgcttttgtc atggatcgac ttttcaggaa attcggtctt tcaggaaaat
     1981 ccttcattcc tatgctgatt gctacaggat gtggagtgcc aggcgtcatg gccagtcgca
     2041 cgattgaaaa cgagcaagat cgtcgtttga cgatcatggt cacaaccttc atgccttgtt
     2101 ctgcaaagtt gccgattatt gccttagtag caggggcctt ttttcctcat caatcttggg
     2161 tagctccttc tgcctatttt cttggtatga ctgcaattat tttctctgga atcagcttga
     2221 aaaaaacacg tttgtttgct ggagatacag ctccttttat catggaattg cccgcatatc
     2281 attttcctca atggtcagcg gtcctaagac aaacctatga ccgcagcaag tcttttgtta
     2341 aaaaagcagg aacgattatc tttgtttcta gtatcgtgat ctggttcagc tcttcttata
     2401 atttttatgc tcaacctgtt tctgaagacc aaagtatctt agcctttttc gggcgaatcc
     2461 tggcagttgt ttttcagccg cttggttggg gaaactggca aggaacagtc gcagcgatta
     2521 caggattggt cgctaaagaa aatattattg gcacatttgg tattttgttt ggtcatgcgg
     2581 aagtatccga aaatggcaga gaaatctggc aagtgctaca acacacatat actcccgcag
     2641 cagcttattc attgttagcc ttcaatttac tttgtgcgcc ttgttttgcg gcaattgggg
     2701 ctatccatcg ggaaatgaat gccaagaaat ggacttggat cgccataggc tatcaatgcg
     2761 gtttagctta tttagttagt tttgtgattt atcagttggg acacttattg gagggtggac
     2821 aggtagcgtt agggacgtat cttgctattt tactgatgat aggactcgtt tatgttttgt
     2881 tccgtcagcc aaaatccaag aaccagtttt atactatatt gtcattagaa ggagaggaat
     2941 aggatggcga cattcttatt aagtgtgtta atctttggaa gtgcaggcgc aattgtctat
     3001 actcgtttaa aatccggtaa gaattgtgat gactgtcaaa cagcttgccc agtaaaaaag
     3061 gaacagatca agcgatgatc agtcgttgca agaggctatt ctcccctttt ttgcttattt
     3121 tgctagagca tagtaagatg agggaaagta aaaaaagaaa gaggaaagaa aatgtctgga
     3181 aagaaaaaaa tcgtccgccc gcgctaccag cagattgctg ttgatttagc cgaacgtatc
     3241 gttgaaaacc gatataaagt gggtgagaag atccatgccc gctcaacact tgccagtacg
     3301 tacaatgctt ctcctgagac tacccgcaag gctatcaatg tgctggtaga tcttggaatc
     3361 atggaagtac ggcatggaag tggggcattt gttgcttcga aggaaaaagc aaaagatttc
     3421 ctattgcaat atcaagatgt acaatccatc caagatacaa aagcagaaat catggcaagt
     3481 gtggagaagc aacaagatga gctgaatcat ttttctcaat tattggatca gctggtcaat
     3541 caaacgaagc gaatacataa gatgaatcct ttacttccgt ttgaattatc tttatcagaa
     3601 aaagcaatgc atctggaccg ttcaattagc gagttgaata tctggcaggc gactggcgcc
     3661 acagttatcg caatttctca taatgaagaa ctgcttgttt cacctggccc ctatgctaaa
     3721 tttacggcag gggacatcgt tcattttatt ggaaatgaat atacactaca gcgtatgaca
     3781 aacttttttt acccggagca tcagcttgca gaatgattgt gtgaaatatt aaaaaaatat
     3841 taacttggct aaacctttta tttacaaaga tttagccaag tttttttcag tttggacaag
     3901 caaatttctt tcatttttga caaagtgact acatgatgtt aacatagtgt ggtcactttt
     3961 ttcagaaaga aaaatgaatt tacataaaaa aggagaattt gtttggtaaa agtagaagta
     4021 aaacatttaa caaagatatt tggtaaaaag acacaagccg ctttagatat gatgaatgat
     4081 caccagccga aaacggagat tttaaagaaa accggtgcaa cggttggtgt gtatgacgtg
     4141 aattttgacg taaaagaagg agaaatcttc gtgatcatgg ggctttccgg gagtgggaaa
     4201 tccacattga tccgtctatt gaatcgtctg attgagccaa cttcgggcag tatctatatt
     4261 gatggagaag atgtatcgaa gttaagcaaa gaagaattgc gagaagtccg tcgtcataaa
     4321 atcaatatgg ttttccaaaa cttcggtctg tttccacatc gaacgatctt ggaaaataca
     4381 gaatacgggt tagaagtccg tggtgtacct aaagaagaac gacaagaaaa agcagaaaaa
     4441 gcattagaaa attctagttt gctttcattt aaagaccaat atcctagtca gttatctggt
     4501 gggatgcaac aacgggttgg cctagccaga gctttagcaa atgatcctga gatcttgctg
     4561 atggacgaag cgttctcggc tcttgatccg ctgatccggc gagaaatgca agacgaatta
     4621 ctggacctgc aagccaatgt tcaaaaaacg attattttca ttacccatga tttgaatgaa
     4681 gctttacgga ttggggacag aatcgccttg atgaaagacg gcgaaatcat gcagatcgga
     4741 acaggtgaag aaatattgac caatccagca aatgattatg tacgtgaatt tgtggaagaa
     4801 gtcgatcgtt ccaaagtgct tacggctcaa aacatcatgg tacctgcatt gacgacgaat
     4861 atcgaatctg atggaccaaa cgttgcattg acaaggatgc gtaatgaaga agtcagcatg
     4921 ctgatggctg ttgacagaaa gagacacctg aaagggatca tcactgcaga tcaagcgtta
     4981 gaagctagga agcaaaaacg tccattgatc gacttcctag atgaaaatgt gacggtgatt
     5041 gggaaagaca tggtggtaag tgatattttc aatattattt atgactcacc gactccactt
     5101 gcagttgtag aggatggaaa attaaaaggt gtcgtcatcc gaggtagtgt gattgaagca
     5161 ttagcagaaa caagcgaggt gagtgaacat gaataatcta ttgaactatc aattaccagt
     5221 ggcaaactgg gtagaaaata tcacagaatg gtttacgaca actttttctg gcctattttc
     5281 atttttacaa acgattggac aggcagtgat gtcggggatt acaaatttac tgttagtgat
     5341 tccagctcct ttgtttatct tgttattgac ggtagcagct ttctttattt ctaagaaacg
     5401 tccaggactt acgttgttca cattaatcgg tctatggttt atttataacc aaggtctatg
     5461 gaatgatttg atgaatactg tcacacttgt tcttttatct agtgtgatct cgatcatcat
     5521 tggggtacca ttagggatct tgatggcaaa aagcagtaaa gcccaaagta ttatcaaacc
     5581 gatccttgac tttatgcaga caatgccagg attcgtttat ttgattccag cagttgcatt
     5641 tttcggtatc gggatggttc caggggtatt tgcctcagtc atctttgctc tgccaccaac
     5701 cgttcgtttt acgaatttag ggattcgaca agttcctaaa gaattagtag aagcttctga
     5761 ttcattcggg agcacaagtc gtcaaaaact attcaaacta gaattgcctt tagctaaaag
     5821 tacgatcatg gcagggatca atcaaacaac aatgctgtca ctttcaatgg tcgttattgc
     5881 ctctatgatc ggtgcacccg gcttaggacg cggcgtatta tcagcattgc aaagagcaca
     5941 agtcgggaat ggatttgtca acggggttgc attagttatc ttagcaatca tcattgaccg
     6001 ttttacacag catttgaacc agccaaacaa taaaaaagct gcaggagcaa caacacagaa
     6061 aagtaaaaaa cgtcaaggac tgatcattgg tgctgtagta gtagtgatct taggtgcgat
     6121 tggtataggg agtttttcat ctgccaaaga aacaaaacgt atcaacttat cttatgttga
     6181 atgggataca gaagttgctt caacaaatgt agtcggtgaa gtattgaaac aaatgggcta
     6241 tgatgtaacg atgacgccat tagataattc aatcatgtgg aaatctgttt caaatggcga
     6301 gtcagatgcc atggtttcag cttggctacc taaaactcat ggttcacaat atgcacaata
     6361 taaagatcaa gtcgaagacc taggtgccaa tttgacagga gcaaaagttg gtttagcagt
     6421 gccagcctac atggatgtaa attcgatcga tgagttaacc gatcaggcag gtaagaaaat
     6481 cataggaatc gagccaggag ctggtgtagt tacggctgct gaaaacacga tccaaaaata
     6541 tgacaacttg aaagactgga aagtagagac atcttcttca ggagcaatga ccgttgcact
     6601 aggtcaagcg atcaaaaaac acgaaccaat tgtagttaca ggatggacac ctcattggat
     6661 gtttgccaaa tatgatctga aatacttaga agatcctgaa aacggaatgg gaagcgaaga
     6721 gcagatccat acgatggttc gaaaaggatt gaaagaagac cagccagaag cttacaaagt
     6781 attagacaat ttccattggt cagaaaaaga tatggaaaaa gttatgttgg aaatcaacaa
     6841 tggaaaagat ccacagcaag cagcaaaaga ctggatcaaa gagaaccaag aattagtcga
     6901 aagctggaaa aaataaaaag acagacacga aataaatgga gcttccaaaa ttggatcaaa
     6961 tatccaaatt tggaagctcc atgttcgtct ctatcttttt tgtctataaa aaaagagaat
     7021 gcgtagctca tcttcgaaac gtccaaagac gccgacaaac tattatgaat ttatgggtgc
     7081 gagtcaaaga gatcgatcgt aggtatgaaa gactaatgtg attaaagttt attatgtacg
     7141 aagtgcattc tcaaaaaata ttaaagaata tacatagttt atatacttaa aagtgattaa
//
LOCUS       DAHUWM010000139         7071 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHUWM010000139 DAHUWM010000000
VERSION     DAHUWM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.141"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            6..305
                     /locus_tag="NK788_002052"
     CDS             6..305
                     /locus_tag="NK788_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770376.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="NK788_002053"
     CDS             378..1739
                     /locus_tag="NK788_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCH6770377.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="NK788_002054"
     CDS             1761..2021
                     /locus_tag="NK788_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770378.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="NK788_002055"
     CDS             2035..2787
                     /locus_tag="NK788_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCH6770379.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="NK788_002056"
     CDS             complement(2881..3486)
                     /locus_tag="NK788_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCH6770380.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="NK788_002057"
     CDS             complement(3539..4513)
                     /locus_tag="NK788_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCH6770381.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="NK788_002058"
     CDS             complement(4891..5610)
                     /locus_tag="NK788_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6770382.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="NK788_002059"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="NK788_002059"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCH6770383.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
BASE COUNT         2167 a         1289 c         1453 g         2162 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tactactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta a
//
LOCUS       DAHUWM010000140         6948 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHUWM010000140 DAHUWM010000000
VERSION     DAHUWM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.142"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1150
                     /locus_tag="NK788_002060"
     CDS             <1..1150
                     /locus_tag="NK788_002060"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCH6770384.1"
                     /translation="IGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNR
                     ANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARS
                     FEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAMMI
                     TQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKELS
                     GRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNLYT
                     GSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEML
                     DSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1172..1924)
                     /locus_tag="NK788_002061"
     CDS             complement(1172..1924)
                     /locus_tag="NK788_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCH6770385.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2288..4954)
                     /locus_tag="NK788_002062"
     CDS             complement(2288..4954)
                     /locus_tag="NK788_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6770386.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGLGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5295..6746
                     /gene="cls"
                     /locus_tag="NK788_002063"
     CDS             5295..6746
                     /gene="cls"
                     /locus_tag="NK788_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCH6770387.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2041 a         1445 c         1266 g         2196 t
ORIGIN      
        1 aatagggatt gatcgtcttt ccttttttat tcctaattta tatttagata tgacagagtt
       61 ggcagaaagc cgtggggatg atcctgcaaa ataccatata gggattggtc aagaccaaat
      121 ggcagtcaat cgtgcaaatg aagacatcat tacactagga gcaaatgctg ccagcaaaat
      181 cgtaacagaa aaagaccgtg agctaatcga catggtcatc gtcggaacag aatccgggat
      241 tgatcattca aaagcgagtg cggtaattat ccaccattta ctgaaaatcc aatcttttgc
      301 tcgttctttt gaagtaaaag aggcttgcta cggtggcacc gcagctttgc acatggcgaa
      361 agaatatgtc aaaaatcatc cagaacgaaa agtactagtc atagcaagtg atattgctcg
      421 ttacggcttg gcaagcggtg gtgaagtgac gcaaggtgtc ggtgctgttg cgatgatgat
      481 cactcaaaac ccgcgtattt tatcgattga agacgacagc gtatttctga cagaagatat
      541 ctatgatttc tggcgtccag attatagcga atttcctgtt gttgatggtc ctttatctaa
      601 ttctacgtat atcgaatcat tccaaaaagt ttggaatcga cataaagaat tgtcgggtcg
      661 aggactcgaa gattatcagg cgattgcttt ccatattccg tatactaaga tgggaaaaaa
      721 ggcattgcaa agcgtattag accaaacaga cgaagaaaat caggaacgtc ttatggctcg
      781 ctatgaagaa agcatccgtt acagccgacg aatcggtaat ctttacactg gttcattata
      841 cctggggcta acttctctac tggaaaattc gaaatcacta cagccaggag atcgcatcgg
      901 tctattcagc tacggttctg gtgcagtaag tgaatttttt acaggttatc tggaagaaaa
      961 ctatcaagaa tatctcttcg cacaatctca tcaagagatg ctagattctc gaacacggat
     1021 cactgttgac gaatatgaaa cgattttcag tgagacgctt cctgaacacg gagaatgtgc
     1081 agaatatact tcagatgtac ctttttcgat tacaaagatc gaaaatgata ttcgttacta
     1141 caaaatataa taaaatcata aaaagaaccc ttcacgagtc gtgaaaggtt ctttttttat
     1201 ctgcggacca aaggagcagt ctataggtca gcttagtcgt actaaggatt tctcgttttt
     1261 cactgaatag tttgcgaatg tacctggtca caacaggatc ttttgcgtac gcgtcatagt
     1321 atagcttcat caattcttca gtaattgatt cctgtgtaaa ataagtaaaa tcttttttct
     1381 tcactttgaa tccttcatca attaagtatg tttcaaattg atccattaca actggcgatg
     1441 tattaggatc ttcttctatc attcctagtt gcttttcgat aggataaaaa acatcatcga
     1501 ttgattccac tactctgatt atcacgcacc atttgcttga ctttttcatc aatttttcta
     1561 attgaataag atgtaccaat gctggatttg ctgctgtaaa aaccatttca tatgtctcat
     1621 ttgattggat aaattcttct aacgactgtt tgataaagga aacatttggc aactcatttt
     1681 tttctgcttc tttttttgca ttttctagca ttcctgaaga aaaatcgatc aaatcatagc
     1741 gattaatttc ttttgctaag gaaagaaatc tgccagatcc tccagcaacg tctgctgctg
     1801 tttcagcagg caggactttc tccgttttca gataattttt tacatcttca gcaatagtta
     1861 cttgcgattc ttgctggatt tcataatatt cttcagcaaa ttcatcccat tctttttcat
     1921 tcatttgatt tcctctaatt ttttgttata tgttttcact tttcggaata aatcattctc
     1981 ctcttttatc tattctatct cttttgatga ataataaaat gattggcttt ccgtctatca
     2041 taaataaaaa aagggactgt gaccaaagtc gtgtcaaagt ccctctttcc gaataaacag
     2101 tgtccaatca gatgaccctc agtattagct caaaatgctg attttacaac ttcttcctta
     2161 aacggtgttc tcctgccaac tcttcggtca caagtcacaa tattcaacaa acatgagaag
     2221 ctgtttgttg aaattgcacc ttgtgactca gaattaaacg gcaggctcac aacctcgttg
     2281 attcgtttta tttatttcct tgtactcgta caatgatcaa tttagtgatt tccatcaaaa
     2341 caattgtcac aagtgcgatt cccatacatg taaagacttc tgtcaatcca aatgctgatg
     2401 ggatagagaa gacttctttc agtcctggga tcattgttaa gcagtaaagc gcgctacata
     2461 caactacagc tagtaagaca cttctgttag agaagaaccc tgcttggata gctgtttgtg
     2521 tgtttgaacg tgctgggaat gtttgcaatg tccgacacca aatcaatgtt gaaaatgaca
     2581 tggctacacc gaattcaggt gaatgctgca tgccaagcca ttgagcaaag ataacagcaa
     2641 tcccaatcag cacaccacga tacgttacag aaatcaatgt tttgtccgta aagattcctg
     2701 aatttggatc tcttggcggg cggttcatcg tagcaggttc agctttttct gtacctaatg
     2761 caattgctgg cagtgaatca ttgactaaat tgatgaacaa cagttgtaat gctgtaaatg
     2821 gattggacca gtcagcaact aaggcaaaga tgatcgcaat gatcgcacct aaatttcctg
     2881 caaatagata agaaatagct ttcttgatat tatcaaacac attacgtcct acttctactg
     2941 cgctgacaat agaagcaaag ttatcatccg tcaataccat cgctgctgca tcttttgcca
     3001 catctgtacc cgttcccata gcaattccga tgtctgcttg tttcaatgct ggtgcgtcat
     3061 ttacaccatc acctgtcata gctgagatat gacctttatt ttgccaagca cgaacgatac
     3121 gaattttatt ctcaggtgaa acacgtgcat aaacagtgac tttttctaaa acttcgttta
     3181 gttgatcttc tgtcaattca tctagttccg tcccggtcaa agccaggttt ccttcttcaa
     3241 aaatcccgat ttgttttgcg atagcaacag ctgttgtttt gtggtcacca gtaatcatga
     3301 ttgttttgat tcctgcactt cttgcttctg ttaccgcctg agtcacttca gcacgtggag
     3361 gatcgatcat agcaagcaaa ccaacaagga tcaaatcgtc ttcatcttca aatgataaat
     3421 caaaattatc aagtggacgg taagcaaaag ctagtacacg taatgcgcgt gttgaaaaag
     3481 cttcattttg ctcttggaat tttttcatca attcatccgt catcggaacg acttcaccat
     3541 ttagcaatac ttttttactg cgttggaaga cgatatctgg tccgccttta acaaacaagt
     3601 aatttttgtt atcaattgtg tgtccagtag acattaattt tcgatctgag tcaaaaggaa
     3661 gttctgcttg tcttgggtag ttgtttcgga tttcctgata aggctggttt aatttttcac
     3721 taaagtcgat aaacgctact tccgtagggt cgcccaattt agtaccgtct tcactgatcg
     3781 atgcatcatt cgctaaaaca ctgatttcga tcaagcgacg ttcgtcagct gtccattttg
     3841 aagggtcatt tgtgaaatcc ccagtatcgc cattacctaa aaagtagtca acaactgtca
     3901 ttttgttttg tgtcaatgta ccagtcttat ctgtacagat gatacttgtc gaaccaagtg
     3961 tttcaactgc tggaagcttc cgaataatgg catgacgttt tgccatttta ttcgttccca
     4021 aagataacac gattgttacg atagactgta atgcttctgg aatagcagct acggctacgg
     4081 ctacggcaaa catcactgcg ctgacgatat ctgcagtcat atctcctgta ccttctccaa
     4141 gataaatacg aaacagttgg atacctaaaa tcaataaaga caatgccaaa atagccaaac
     4201 ttagtttttt actgaattta tccagacctc gttgtaacgg agtgacttgc tcagttgttg
     4261 attccaaaag gccagcaact tgtccgatct ccgtattatt cccagttgaa gtcactaaga
     4321 atttaccgag tccgtaagtt acgatcgttc cactgaatac catatttgta cgatctccga
     4381 ttggtaccgt acttgtgata tccgttactt ctttttctgc agaagtcgac tctcctgtca
     4441 gcataccttc atctactttt aatgatcccg catctagtaa acgtccatca gcaggtacat
     4501 aatcgcctgc gtctaacaac acgatatctc ctaccacaag ttcgtttgcc ggaatcgttt
     4561 tttccttacc atcacgaatc actcgagcat cgggcgctga aaggtttttc aacgcttcta
     4621 aagaaccttc tgcttttttt gtttggacaa cactgattat agaattcaac atcaatactg
     4681 caaaaataac taagaattcg atgtagtcac ccatgaccat ctgaatcagt gcgacaatca
     4741 gcaatacgat aaccattgca tctttgaaag tttctaagaa caatttccaa gttggcttct
     4801 tcggcttttc ttttagctgg ttcaagccat ctctttctaa tttttccttc gcttgcgtgg
     4861 tgctcaatcc atcggttgag ctctgcgttt gctgcatcaa cgagtcgatc gtttctttat
     4921 agaccgcttg ttgtgcttgt tccttgtttt ccaaatttca tcccccattt tttgatcttg
     4981 gccctagtcc tgtctgaaaa aaaagagacc taagacaaat acgcatttgt cttaggtctc
     5041 accatttcat acaataccag aaaaccattt ggttaactta ctgttgatat tatcgcaaca
     5101 aacgttgccg gttactcccc taaggagata ttcaactgta ctaatatcct accttattaa
     5161 cctacaaaaa gcaactactt tagcccgatt tacctacatt ttcatgaaag gagtgaaaag
     5221 tatattcacc ttggtaggct tctttcttct atcatgctat actaatgtta ttgtatgtaa
     5281 ggaggaacat ttttgtggta tctagtatta taaccgccct ttatctatta aatgcactta
     5341 ttgctttggt ggctattttg atcaaacccc gagatgtagc agccatttgg gcatggctct
     5401 tagtattgtt tgcccttcct ggggtgggct ttgtattata tttatttttc ggacgtggat
     5461 taacggataa gaaaaaattc tatctccgac aaagtgactt gaaagaatta gaaaactttc
     5521 agtcttttag aggagatacc attgaacatt acgatcctga catgggcgat aaagacaaac
     5581 agcaatttgt tgacttcttc tcttcattaa atcgtatgcc gctgacaaga atgaattctg
     5641 tcactcttct cacagacgga caagagaaat tggattcact gcttcaagat ctaaaaaaag
     5701 ccaaacattc gatccatatc gaatattacg catttgtgac agataatatc ggccagcaag
     5761 tcttacatgt tttagaagaa aaagccgcag aaggcgtgga agttcgaata ttatatgatg
     5821 catttggctc tcatggcaca aaagcaaaag atttcaatcg tctaatcaaa aatggtggac
     5881 atgtccatac atttgttacc tcacaaaggg cattacttcg tttccgattg aattaccatg
     5941 atcaccgaaa aatcgttgtg atcgatggaa agattagtta taccggtggt ttcaatattg
     6001 ccaatcaata tgtaaataca acaaaaaaat tcggctattg gcgcgatacg catatacgga
     6061 ttttcggtgc cgcttcttct ttgctccagc ttcgcttctt aacagactgg aacgtctcgg
     6121 tacctgaaga aaaaaaggtc ggctatcatt tgaattattt ctttaaaaaa gcagatcgag
     6181 atgaatctaa gcttgctgat acatccatcc agcttgtttc aagcggaccg aataacgaaa
     6241 gggaacaaat caagctttca tttatcaaat tgattacttc tgctaaaaaa cgtgtttgga
     6301 tacagacacc ttaccttgtt cctgatgaaa gtgtcattgc tgctttaaaa atcgcaactg
     6361 cctctggtgt agatgtgaaa atcatgattc ccaacaaacc ggatcatcct tttatttatc
     6421 gagcaacaca atattatgct cggcagctga tcaaggaaaa tgtacaaatc cttgtctatg
     6481 agaacggctt cctccatgca aaaacattga taatggatga tgaaatctgc atggtaggtt
     6541 cagcaaatca agatattcgt agctaccgat tgaattttga aacaagtgct gtcatttacg
     6601 atcctgagtt tttagaagaa cttgctactc agttcaaaga agatgagaca cattgttcat
     6661 ccatgacaac tgaaacagtc aaggaaatgt ctaactggct attattcaag caacaaattt
     6721 ctcgattatt ttctccaatc ctataatttt tttattcaag tagagaaaaa tatactaaga
     6781 ttaaagggac tgtgacaaaa atcttgtcac agtcccttct tccgaacaaa cggtgttctc
     6841 ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa
     6901 cttgttcctt gtgactcgga gctggacggc aggctcacga cctctttc
//
LOCUS       DAHUWM010000141         6937 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHUWM010000141 DAHUWM010000000
VERSION     DAHUWM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.143"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            170..1027
                     /locus_tag="NK788_002064"
     CDS             170..1027
                     /locus_tag="NK788_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCH6770388.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1020..1922
                     /locus_tag="NK788_002065"
     CDS             1020..1922
                     /locus_tag="NK788_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCH6770389.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1955..2305
                     /locus_tag="NK788_002066"
     CDS             1955..2305
                     /locus_tag="NK788_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCH6770390.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2354..3276
                     /locus_tag="NK788_002067"
                     /pseudo
     CDS             2354..3276
                     /locus_tag="NK788_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            3354..4247
                     /locus_tag="NK788_002068"
     CDS             3354..4247
                     /locus_tag="NK788_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6770391.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4285..4848
                     /gene="amaP"
                     /locus_tag="NK788_002069"
     CDS             4285..4848
                     /gene="amaP"
                     /locus_tag="NK788_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCH6770392.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4862..5050
                     /locus_tag="NK788_002070"
     CDS             4862..5050
                     /locus_tag="NK788_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCH6770393.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5063..5965
                     /locus_tag="NK788_002071"
     CDS             5063..5965
                     /locus_tag="NK788_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6770394.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5997..6239
                     /locus_tag="NK788_002072"
     CDS             5997..6239
                     /locus_tag="NK788_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6770395.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6495..6866)
                     /locus_tag="NK788_002073"
     CDS             complement(6495..6866)
                     /locus_tag="NK788_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770396.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2527 a         1182 c         1386 g         1842 t
ORIGIN      
        1 aataaaaagc catgaaaaac gaatagttag cgcttaccga tgcgttttgt gataaaaaaa
       61 ttttaaaata ttcaatgaaa acaaagcata tattttgctt taaaaccaaa tgaggaatat
      121 tctagatgta gaacaggtta gaaaaagatt agaaaaagga gtgattttga tggagaagag
      181 atacggcgta gttcctgtgg taactgcttc agacgaaaat tatgctcctt acttgagcgt
      241 gatgatcgca actgcattag aaaattgcaa taaaacaaga cgcataaaat tttatgtgat
      301 tgacgatggt ctatcagaat atagtaaaga aggattggaa gaaacggtaa ataaatattc
      361 aagtaatgca agtatccaat tcctgacagt ggaaaaagat atctacgaag acttcctagt
      421 cagtgaccat atcacaacta ccgcttactt aagaatttct ttaccaaact tattagctaa
      481 agaggattat aagaaagtcc tttacttaga ttcagatgtg ttagtactag acgatatcgt
      541 aaaactatat gatgagccgt taaatggaaa aacaatcggc gctattatcg atccaggcca
      601 agtgaaagct ttggaaagat taggaatcga ttccgatgac ttgtacttca attcaggagt
      661 gatggtgatt gacattgatc aatggaataa aaaagaaatt acagaaaaaa ctatccatta
      721 cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa gatgctttga atgctgtact
      781 ttatgaagat tgggaacaac ttcatccaaa atggaacatg cagacttcgc tgatttttga
      841 acgtcatcca gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc
      901 ttcgatcgtt cactttacag gacatgataa accttggaat acgttgaaag atcatccgta
      961 taccaattta tacttaaaaa aattagcaca cagtactcta acgaaagtag gcgagatcaa
     1021 tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac aaaattcgta ccacatttag
     1081 ctgcattatt tgtctcagta ttagataatt gcaacccgtc taaatttgtt cggttctatg
     1141 tgattgacga tgatatcgat tttgaaagta aacagctttt acgcttctca gtgaaaaacg
     1201 cacgaatgaa ctcagatgtg gaattcttga aaatcaataa agaattcttt acaaatgtgg
     1261 taatcagcga tcgtattcca gaaacagctt attatcgtat tgcgattccg gaattgttcc
     1321 gcggcacaga agttgaacgt atcttatata tggattgcga catgatcgct ttacaagata
     1381 tctctaaatt atggcgtctt gacttcggtg attcaatcgt agcagctgtt gaagacgctg
     1441 ggttccatca acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact
     1501 caggtttgat gctgatcaat gtgaaaaaat ggttagatga aaatattacg caaaaagtat
     1561 tagatttcat cgaacataat ccagaaaaat tacgattcca tgaccaagat gcattaaatg
     1621 ctatcttaca tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa ggctatatca
     1681 tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa gaaactcgca
     1741 acaatcctta cattatccat ttttcaggac atgtgaaacc gtggagcaag gattttgaag
     1801 ggccaacgaa gaaatattac gaaaaatatg cagggatgac ggctttccgt tgtgtagcta
     1861 aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat
     1921 aatagaagac aacaactgaa gagggggagt aggaatggaa attacaatgt ctaaaaatgc
     1981 agtagagacg ttaattgaaa aggttgggga aaatacaaaa atcgcactcg cgcttatcaa
     2041 tgattccgat ccatttctaa gggacaaagg tgcttttgca aaaggcagct tttttcaaat
     2101 cattcctttt gtttcggaat ttggagaata tgcgactaaa atcgagcatc cattattgga
     2161 catctataca tctaaattag agcaaaatta tttcggtaaa cgattgaaca tggacttcaa
     2221 caaacagctt gacagctttt cattagaaaa tgaaatcgca gtactcgatt ataatatcaa
     2281 attaaagaac tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt
     2341 gagatactac tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa
     2401 ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga
     2461 gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg
     2521 cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc
     2581 agataatcgt atcgaaaaaa attaacgcca atatcttttg tactttccga taatctcttg
     2641 attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa gcatggaaat
     2701 catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta cacccattac
     2761 gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc agctcgtaaa
     2821 acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat cgtatatatc
     2881 gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc tgaaactttc
     2941 atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca gcgacaaaca
     3001 gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct attcaacggg
     3061 atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc attaatcatt
     3121 tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt accgggtaaa
     3181 agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc agtagtttta
     3241 ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa acaggtatca
     3301 aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca gcaatgacag
     3361 aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct aaacaagatc
     3421 aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga gaagaatcct
     3481 atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga gattcaggga
     3541 tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct cttcaattct
     3601 tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagat gcaggacgta
     3661 agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa atcatagata
     3721 aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga cagcaaattt
     3781 cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt atgataaaca
     3841 tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca ggaggagcaa
     3901 tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta gattacgcag
     3961 caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt gcacctaaag
     4021 gaatccgtgt aaatggtgta gcacctggtc caatctggac accattacaa ctagacgaag
     4081 gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc
     4141 agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca agttatgtta
     4201 cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa gttgatcgtt
     4261 tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga
     4321 acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac ctatccattg
     4381 ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt gcaacaatat
     4441 gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact tgttttcctg
     4501 ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga gttgacgttg
     4561 gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga attcatccat
     4621 tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag
     4681 ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga aatcgaaaat
     4741 gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa atacacaggc
     4801 tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg ggggaccaaa
     4861 aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac ttggattgat
     4921 attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct tgatcatggc
     4981 agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag atcactattt
     5041 taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca ataaaatgaa
     5101 caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat caaatggtgt
     5161 gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag tagtccctaa
     5221 cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc
     5281 aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt ctaaacctgt
     5341 ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta gttctgacca
     5401 cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa agcatgattc
     5461 tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg tgcaaaaaat
     5521 catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg gtttcttctc
     5581 taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg aaacagaagt
     5641 cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca aagatatcga
     5701 aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata tgacacattt
     5761 agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa
     5821 agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc
     5881 ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga
     5941 aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg
     6001 gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga gcaatcacga
     6061 ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt ggatcagcac
     6121 ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca atcgtgccat
     6181 cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa
     6241 aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa gaacagccaa
     6301 acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga tgcaagcttt
     6361 tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag cctcttattt
     6421 atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt ctttattgat
     6481 acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat aggaaaggtg
     6541 aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg gatcgtatga
     6601 tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa gacaaggaag
     6661 aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt aaagtaatct
     6721 agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc atgttgctct
     6781 gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac gagtaatgaa
     6841 agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt tcgccctcat
     6901 tttaaaataa cgatatttgt ttgtgaagct ttttcgt
//
LOCUS       DAHUWM010000142         6811 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHUWM010000142 DAHUWM010000000
VERSION     DAHUWM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.144"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(59..241)
                     /locus_tag="NK788_002074"
                     /pseudo
     CDS             complement(59..241)
                     /locus_tag="NK788_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            228..425
                     /locus_tag="NK788_002075"
     CDS             228..425
                     /locus_tag="NK788_002075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770397.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(468..1043)
                     /locus_tag="NK788_002076"
     CDS             complement(468..1043)
                     /locus_tag="NK788_002076"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCH6770398.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1198..1724)
                     /locus_tag="NK788_002077"
                     /pseudo
     CDS             complement(1198..1724)
                     /locus_tag="NK788_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2181..3692
                     /gene="gadC"
                     /locus_tag="NK788_002078"
     CDS             2181..3692
                     /gene="gadC"
                     /locus_tag="NK788_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HCH6770399.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3705..5105
                     /locus_tag="NK788_002079"
     CDS             3705..5105
                     /locus_tag="NK788_002079"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCH6770400.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5270..6710)
                     /locus_tag="NK788_002080"
                     /pseudo
     CDS             complement(5270..6710)
                     /locus_tag="NK788_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2111 a         1168 c         1229 g         2303 t
ORIGIN      
        1 caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggtt
       61 actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta tggcttctat
      121 ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct agtgattctt
      181 cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttattg aaagattcga
      241 ttgtagaacc agaaggaatg gagaacactt ttgaacttta caacaaaact actgttgata
      301 aattaattcc aattttagtt ccaactgatt taaatggtca tgatgtttta cttcgtttta
      361 acggattttt aggtgaggat tatagatacc taaaaatttc tgatcctcaa cctttaacag
      421 aataataaaa aacgtgtaat caattatttg attacacgtt ttttatttca atcttcgttg
      481 tagtagtggt tgcgacatac cgaaaggtaa tcttcattac ctcctactac aatctgttca
      541 cctgaataaa ctggaatacc atcaacaata cgagcattca ttacagcctt tttattgcaa
      601 aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa atgtcaataa tgcttcggat
      661 cccttgaaga gattattttg aaaatcattc ttcaatccat agcaaattac tggtacttcc
      721 tcattcatta cgataagtga tagatcaata atattctctc tacttaaaaa ttgagactca
      781 tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa tctccactaa ttctccgtac
      841 acatccattt cagaagtaat tgcaatagct ggtttcgaaa cacctatacg actctcaact
      901 tttccaaatc ctgatcgagt atctattgcg cttgtcatta tgataactct cttcccttgt
      961 tcttcataat tatgagccac tttcagtagc tcaatactct ttccactatt cattgttcca
     1021 tatctgtaaa ataattgtgc cattaaattt attcccttct tttacaattc aattattcct
     1081 taattccatc tgcttttatg cagctgctcg tttattcctt gatatgtcta taacatctac
     1141 tatccctaaa accaatttat aagccattag agatggtatt cctgaacatc tctcatattg
     1201 ttttttgagt ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc gacatactta
     1261 tcatttagag ttctaacttt aaatgcattt acttcttcag tcagcacttt agtcatgttg
     1321 gacatggttt ggggcgtgta gtgatgccca tacattttct caatcaagtc tgcgatttcc
     1381 gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaatgt gtcattcgtc
     1441 cgtctataag caggaacagt ctgttgcttg aactcgccat tgcggtcgcg cggaatctgg
     1501 agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt acgagaatta
     1561 cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt taactccgtt
     1621 ttgagaagcg tattgacagc tttttccaaa tggacacgga ataatccatt caaatcgcct
     1681 ttgttcgcta gagtctttag gatttctgta gtaaaatcgt tcatatggaa gtcctctttt
     1741 ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt ctatttacac
     1801 aaaatatttt acactctcaa caaaagtatc ttcgattaat acttgctcaa gttatgtgca
     1861 gctacgaaaa agtaggtacc gaggtacttt ctttttcgca gctttttttg gacttatgag
     1921 aagtacattc gctggaagaa tacgatataa aacgagcttt ttcactaaag atattaatag
     1981 agtgaaagaa acaagctttt gttgtcagtt ttttcacttt tctaaaaatg aaaataaaaa
     2041 attctggaag tggagaagaa ttttgcttgc tatactttgt cttgtagaaa agaggtggac
     2101 aaaataatgc aagctcaaat aagcgatttt ccataacctt ggtaggaatg acaaattaaa
     2161 taggaaaggg aagtaataat atgaataaaa aaaagttatc tctattcggt ttttttgccc
     2221 tgacagcttc catggttttg actgtttatg agtatccgac ctttgcaaca tcacaattac
     2281 atttggtttt ctttcttctt ttgggaggta ttttatggtt tctaccagtt gctttatgtg
     2341 ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg gattttcagt tgggttagtc
     2401 aaactttagg agaacgtttt ggttttgcag ctattttttt ccaatggttt caaattacag
     2461 taggttttat tacaatgatc tacttcatac taggagcgct gtcgtatgta attaattttc
     2521 cagagcttaa tacagatcca tttatcaagt ttattggtgt attagtcatt ttctgggtac
     2581 tgactttttc acagttgggc gggaccaatc gtaccgctaa aattgctaaa gcaggatttg
     2641 tcattggaat tgttattcct tcgatcattc tctttggttt agcagcagcc tactttatcg
     2701 gcggtaatcc gattcaaatg gctattaatg cgaaagcttt tattcctgac ttttctcagc
     2761 tttcaacttt agtagtattt gtatctttta tattggctta tatgggcgta gaagcttcag
     2821 catcacatat taatgagctg gaaaatcccc aaagaaacta cccattggca atgtttttac
     2881 tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt ctcagtagca gcggttattc
     2941 cacaacatga cttgtctctc agtgctggtg taatccaaac atttgaaacg cttattttac
     3001 attttaacag tcatttacat tggttggtaa aagtgattgc tctcatgatt gcctgtggcg
     3061 ttatgggtga agttagttct tgggtagttg gtccatcacg agggatgttt gcagctgctc
     3121 agcgaggtct gttaccagtt ttcttccgga aaactaataa acaagacgtt ccagttccgt
     3181 tgattatgat acaaggaatt atcgttacta tttgggcagc agtcttaact tttggtggag
     3241 gaggcaacaa tttatcattc ttagtagcaa tctccttaac tgttgtcatt tatctcgtag
     3301 gatatttact atttttccta ggttactttg tattagtttt taagaaaaaa gacttaaaac
     3361 gaacttacaa tgtaccagga aaaactgttg gcaaaacgat tatcggtgga attggtttta
     3421 ttttatcagt gttcgcattg cttatctctt ttgtaccacc agcttcaatt gcagctagtg
     3481 agcaccgaac ataccaaatc atactactta ttagctttgt agtgactgtt cttttacctt
     3541 ttatcattta tgaagtacat gataaaagtg ctcacacaac tattgaggaa cctactcacg
     3601 tgaaagctga agacgttaat cccgctgttt atccagctgc tagaggagag caccacgtta
     3661 caaaaaatga tgaacatatt ttaaaacatt aggaggaatt cattatgtta tacggaaaag
     3721 ataatcaaga agaaaaaaac tatttggaac caatttttgg ctctgcaagt gaggatgttg
     3781 acttgcctaa atataagtta aacaaagaat ccattgaacc acgaattgct tatcaattag
     3841 tacaagacga gatgttggat gaaggaaatg cgcgattaaa cttagctact ttttgtcaaa
     3901 cgtatatgga acctgaagca gtgaaattga tgacccaaac gttagaaaaa aatgcaattg
     3961 ataaatcaga atacccacga acaacggaaa ttgaaaaccg ctgtgtaaat atgattgctg
     4021 atttatggca tgctccaaat aatgaaaaat tcatgggaac ttcaacgatc ggctcttcag
     4081 aagcctgcat gctgggtggt atggccatga aatttgcttg gcgtaaacgt gctgaaaaat
     4141 taggtcttga tattcaagca aaaaaaccta acctggtgat ctcttctggt taccaagttt
     4201 gttgggaaaa attctgtgta tattgggatg tggaactgag agaagtccca atggatgaaa
     4261 aacatatgtc aattaatcta gatactgtca tggattatgt ggatgagtac acaattggta
     4321 ttgtaggtat tatgggtatt acttacactg gtcgttatga tgatatcaag ggtctgaatg
     4381 atttagttga agctcacaat aaacaaactg actataaagt atacattcat gttgacgctg
     4441 catcgggtgg cttttatgca ccatttactg aacctgatct agtttgggat tttcaattga
     4501 aaaatgttat ctcaattaat tcttcaggtc acaaatatgg tttggtatat ccaggtgtgg
     4561 gttgggtctt atggcgtgac caacaatact taccagaaga attagtattt aaagttagtt
     4621 acttaggtgg agaaatgcca actatggcta tcaacttctc tcatagtgca gcacaactaa
     4681 ttgggcaata ctacaacttt gtacgctatg gctttgatgg ttatcgtgat attcaccaaa
     4741 gaactcatga tgttgctgtt tatttagcca aagagatcga aaaaactggt atttttgaaa
     4801 tcattaatga tggatcagaa ttgccagttg tgtgctataa gctgaaagaa gatcccaatc
     4861 gcgaatggac actatatgat ttatctgatc gtctgttaat gaagggatgg caagtcccag
     4921 cctacccatt acctaaagac ttggatcaat taattattca acgcttagtt gttcgagcag
     4981 actttggaat gaacatggct ggtgattatg tacaagatat gaaccaagca attgaagagt
     5041 tgaataaagc tcatattgtt tatcataaaa aacaggatgt gaaaacgtac ggctttactc
     5101 actaaaattc ttcagagaaa tataaaagaa gttaattaac taataaaagg agttgtgatt
     5161 accacacagc tccttaaatc tatttgaata aatatatgat attcatttgt tacactctca
     5221 atcttagctg ttgttctatt catcattact cttattgtaa gtagttcagt caaatttgct
     5281 tgttcaattg atcgataaca gtatgaattg tttgaaaagt aatcggatat ataattgttt
     5341 tctcactacc taagccttct tggtataaag aactatcgca aagtataatc cgctgagtac
     5401 aaaagaaagt gtcgtctact atttctctaa gttgtttaca aatgatatta ctaatagaaa
     5461 agtgttctcc caaaatgtct tctaactgtt gtcgatagta taaataccta aagtctgacg
     5521 gagctactat gaacacttca attggagatt tccgtttatg ctcatttaaa agacttaaac
     5581 taatggttag tttacgaata gaattctcat tccaacgcag aaataaattg ttttgttttc
     5641 gccattgttc aagaatttca tgaacttcta agtacaaagc gcgttgctct tcatttaaca
     5701 agtaagtctt atcaggttga aatatctgaa tatcagccca aagtgagcgg ataaacggga
     5761 gaagtgcttt atcaaaaagt atctgatttt cttcagatat attcagagat aagataagtt
     5821 tttgactgag ctcaatcacc aaagggttat ctgctacatg gttttgataa acaacgttca
     5881 agtctttttg taacaattca aagttatcgc atagataatt tctagagttg aatagaaagt
     5941 agatataaag gcattcatct tcgtcatgac ataaagagaa agaaagttgc tctaatcctt
     6001 tctgtattag ctgaaacaag ggagtatgtc gggcaactct tttttcttcc tgactaaaat
     6061 atattgggtt agtcttagat ctctgcaaac cgatagcgat acctaataaa ataaattgtt
     6121 tttcaactgt agggtaactt cttgaaaaaa aatgtttttc tacataatca atcaattgtt
     6181 gaccgctttg aataattgat ttatttagct ggatcaactc tgtccgattt gtctcataga
     6241 ttatcgttag taacaaactt cgataatcct tttcaggaat ttcaaaatgg ccatcagcat
     6301 ttaagtactg catttgttta aaaaaattaa tcaactcatt tcgtaagttg taaactttgc
     6361 ttaacgaaat gaaggtagta tcagctatat tactccaatt gtgttcccct gataaataat
     6421 gtcctagtac attgaaaaag tcagagcgct gatagatgag ttgagtgagt gccaacctcg
     6481 aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga tttctccact gaaataattt
     6541 gctgatggag tatgtcggta atgcattcca agtcgttagt gacagacagt gagtgggcaa
     6601 acgttcaatt gatctgctat ttcgcgaatc tgaatggtcc ctttttcgtg taattgctca
     6661 caaatgtaaa cttgacgaaa aatacttttc tccagatagg tttccaacat agtactcctc
     6721 ctagtcattt tatacataac tattataact ctaccaatcg gaatagtcct gtaatatatt
     6781 gaatcaaaaa tataaattat aaaataatta g
//
LOCUS       DAHUWM010000143         6793 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHUWM010000143 DAHUWM010000000
VERSION     DAHUWM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.145"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(83..1819)
                     /locus_tag="NK788_002081"
     CDS             complement(83..1819)
                     /locus_tag="NK788_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCH6770401.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1816..2520)
                     /locus_tag="NK788_002082"
     CDS             complement(1816..2520)
                     /locus_tag="NK788_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6770402.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2651..3154
                     /locus_tag="NK788_002083"
     CDS             2651..3154
                     /locus_tag="NK788_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCH6770403.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3114..3452
                     /locus_tag="NK788_002084"
     CDS             3114..3452
                     /locus_tag="NK788_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCH6770404.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3473..4891
                     /gene="ffh"
                     /locus_tag="NK788_002085"
     CDS             3473..4891
                     /gene="ffh"
                     /locus_tag="NK788_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCH6770405.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5003..5581
                     /locus_tag="NK788_002086"
     CDS             5003..5581
                     /locus_tag="NK788_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCH6770406.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5585..6106
                     /locus_tag="NK788_002087"
     CDS             5585..6106
                     /locus_tag="NK788_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCH6770407.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6185..6739)
                     /locus_tag="NK788_002088"
     CDS             complement(6185..6739)
                     /locus_tag="NK788_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCH6770408.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         2184 a         1279 c         1418 g         1912 t
ORIGIN      
        1 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
       61 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
      121 ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa
      181 tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa
      241 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
      301 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
      361 attggacagc attttcaatt agatttttga taattggata aaggatatct gttttagtag
      421 taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct
      481 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
      541 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
      601 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
      661 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
      721 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
      781 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
      841 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
      901 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
      961 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     1021 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     1081 gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa
     1141 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     1201 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     1261 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     1321 cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta
     1381 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat
     1441 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     1501 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     1561 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     1621 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     1681 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     1741 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     1801 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     1861 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     1921 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     1981 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     2041 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     2101 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     2161 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     2221 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     2281 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     2341 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     2401 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     2461 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     2521 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     2581 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     2641 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     2701 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     2761 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     2821 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     2881 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     2941 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     3001 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     3061 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     3121 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     3181 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     3241 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     3301 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     3361 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     3421 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     3481 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     3541 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     3601 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     3661 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     3721 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     3781 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     3841 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     3901 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     3961 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     4021 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     4081 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     4141 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     4201 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     4261 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     4321 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     4381 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
     4441 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
     4501 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
     4561 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
     4621 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
     4681 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
     4741 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
     4801 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
     4861 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
     4921 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
     4981 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
     5041 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
     5101 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
     5161 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
     5221 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
     5281 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
     5341 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
     5401 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
     5461 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
     5521 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
     5581 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
     5641 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
     5701 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
     5761 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
     5821 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
     5881 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
     5941 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
     6001 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
     6061 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
     6121 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
     6181 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
     6241 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
     6301 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
     6361 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
     6421 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
     6481 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
     6541 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
     6601 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
     6661 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
     6721 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
     6781 aagataagta act
//
LOCUS       DAHUWM010000144         6767 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHUWM010000144 DAHUWM010000000
VERSION     DAHUWM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.146"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..292
                     /locus_tag="NK788_002089"
     CDS             <1..292
                     /locus_tag="NK788_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6770409.1"
                     /translation="IITVDKEGRLTNVDKVRGRNKSIQTIVNETVKTIVAPLDQIVYV
                     AYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYGCIAIFSMGISR"
     gene            complement(421..2043)
                     /locus_tag="NK788_002090"
     CDS             complement(421..2043)
                     /locus_tag="NK788_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6770410.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2292..2813
                     /locus_tag="NK788_002091"
     CDS             2292..2813
                     /locus_tag="NK788_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCH6770411.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(2999..4729)
                     /gene="spxB"
                     /locus_tag="NK788_002092"
     CDS             complement(2999..4729)
                     /gene="spxB"
                     /locus_tag="NK788_002092"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCH6770412.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            4894..5712
                     /locus_tag="NK788_002093"
     CDS             4894..5712
                     /locus_tag="NK788_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCH6770413.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(5815..6012)
                     /locus_tag="NK788_002094"
     CDS             complement(5815..6012)
                     /locus_tag="NK788_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770414.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6181..6759
                     /locus_tag="NK788_002095"
     CDS             6181..6759
                     /locus_tag="NK788_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCH6770415.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         1977 a         1255 c         1327 g         2208 t
ORIGIN      
        1 tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa
       61 caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca
      121 aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt
      181 ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag
      241 tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta
      301 actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat
      361 agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca
      421 ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata
      481 tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa
      541 tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa
      601 gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac
      661 aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc
      721 agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa
      781 gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa
      841 aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt
      901 tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa
      961 tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa
     1021 attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta cattttctac
     1081 ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct
     1141 agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc
     1201 ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat
     1261 ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc
     1321 caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat
     1381 atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc
     1441 aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg
     1501 ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac
     1561 cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag
     1621 accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc
     1681 tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat
     1741 cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag
     1801 tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg
     1861 tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt
     1921 tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat
     1981 attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat
     2041 caattttttt cctcactttt atcattatta gttttgacaa gtcttataga attatatagg
     2101 ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga
     2161 aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca
     2221 gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag
     2281 aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt
     2341 tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg
     2401 tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca
     2461 gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata
     2521 ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg
     2581 tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa
     2641 gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat
     2701 taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg
     2761 gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat
     2821 caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct
     2881 tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa
     2941 aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc
     3001 acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt
     3061 tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg
     3121 gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct gattttgcag
     3181 ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga
     3241 gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt
     3301 ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa
     3361 taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc
     3421 catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc
     3481 ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt
     3541 tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg
     3601 catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt
     3661 cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg
     3721 ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg
     3781 tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat
     3841 cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt
     3901 cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag
     3961 cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta
     4021 gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc
     4081 cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca
     4141 aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg
     4201 tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag
     4261 ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt
     4321 gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta
     4381 gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta
     4441 gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc
     4501 ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga
     4561 gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata
     4621 gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc
     4681 agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact
     4741 cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa
     4801 atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt
     4861 agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa
     4921 ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc
     4981 gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt
     5041 gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa
     5101 gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt
     5161 gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa
     5221 gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg
     5281 cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat
     5341 aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac
     5401 ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag
     5461 gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg
     5521 ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca
     5581 atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa
     5641 cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg
     5701 gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc
     5761 gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca tacattaata
     5821 atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat
     5881 tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact
     5941 gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt
     6001 tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag
     6061 ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc
     6121 aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc
     6181 atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt
     6241 ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt
     6301 taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat
     6361 catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc
     6421 tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat
     6481 agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat
     6541 atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg
     6601 ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta
     6661 gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg
     6721 atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatcc
//
LOCUS       DAHUWM010000145         6743 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHUWM010000145 DAHUWM010000000
VERSION     DAHUWM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.147"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            142..1152
                     /locus_tag="NK788_002096"
     CDS             142..1152
                     /locus_tag="NK788_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6770416.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1221..2165)
                     /locus_tag="NK788_002097"
     CDS             complement(1221..2165)
                     /locus_tag="NK788_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCH6770417.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2149..4869)
                     /locus_tag="NK788_002098"
     CDS             complement(2149..4869)
                     /locus_tag="NK788_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6770418.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4866..6086)
                     /locus_tag="NK788_002099"
     CDS             complement(4866..6086)
                     /locus_tag="NK788_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCH6770419.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6367..>6743)
                     /locus_tag="NK788_002100"
     CDS             complement(6367..>6743)
                     /locus_tag="NK788_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCH6770420.1"
                     /translation="KDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEI
                     VPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKA
                     DYVTVSNDKDGVSQAFERILTI"
BASE COUNT         1989 a         1280 c         1103 g         2371 t
ORIGIN      
        1 agaaaagttt gcgtatttcc taggaatgtg atgaaatttt gaattttgtt tgaaactacc
       61 acgacaacgt ttttaaagga aatgggatat ggtatagtta atatataaaa ataataacta
      121 attacaggag tgcaaatttt aatgaaaaaa aaatcaatta ttttggcagc tactagtgcg
      181 ttggctgttt taactctagc agcttgttca ggcgatacta ataaagatat cgcaacgatg
      241 aaaggcggaa cgatcactgt atctgatttc tatgatgaag caaaacttga atcatcgaac
      301 caatccttag tacaacggat gatcatttat aaagtattca ataacaaata tggcgataaa
      361 gtaaccgata agcaagtgga tgcagaatac gacaaacaag cgaaatcatt aggtgatact
      421 tttgaatcac aattagaagc tgctggatat acgaaagata cttataaaga atatattcgt
      481 aacaatttag cttttgaagc tggcttgaaa gctcacgtag atattacaga tgacgatttg
      541 aaaactgctt ggaaatcttt ccatccagaa gtagaagcac aaatcatcaa attatcaagt
      601 gaagatgaag caaaagatgt gaaaaaatca gcggatgatg gagatgattt ctctaaatta
      661 gccaaagaca aatcaactga tacagagaca aaagaagatg gcggtaaagt taaattcgac
      721 tcaacgacaa caactattcc tgcagaagta aaagaagcag ctttcaaatt gaaagacggt
      781 gagatctccg atgttattac tacaacaaat cctacttctt atgcgacaga atactacgtt
      841 gtcaaaatgg tgaaaaacca aaataaaggc aatgacatgg ataaatacaa agatcaacta
      901 aaagatatcg ctactgaaac taaactaagt gacaatgcat tcacaactaa agtaatcggt
      961 gaagaattaa aagatgcaaa tgtaaaaatc aaagatgatg cctttgaaaa tgtgttgagt
     1021 gcattcacaa caacttcatc aagtacgaaa gactcaagtg aaactacagc ttcaacaaag
     1081 agttctgaca caaaatcaac tgattcaaca aaagaatctt caactgaaga aacaactgat
     1141 tcatcaaaat aaaagaaaac aaaaaaagcg aagacttatg tctcgctttt tttgttttga
     1201 atgaaaaaaa taaatttttg ctataaatct tttggtacat agaaacttcg gttgtccata
     1261 ccgaaaatcc gatcggtgta ttttcctggt tctgtttgac gaatcgaagt caacatcatc
     1321 tgcatcgaag catcgatatt gtcgatttga tgtaatattt ctgcttccat tattcttgga
     1381 cgtacaggtg agccatattc taataagcca tggtgtgcta aaaccatatg acgtaataca
     1441 agaacatctt cgtcagcttc gctgattttc aacgcaagac aggctttagt tatttcttcg
     1501 tcaatcaata ccagatgacc gatcaaattt cctgcaagcg tatattctgt cgacatagga
     1561 ccggaaagtt caatcacctt tcctaaatca tgcaaaatga taccagcata caacaatgct
     1621 cgattcagct caggatattc atcggcgatt gatttcccta agtgaagcat tgtcaaagta
     1681 tgataagcta atccactggc aaatgcatga tggtttcttt ttgctgccgg aaactcaaaa
     1741 aattgttttt gatatttatt aagtagatag cgaacaatac gattccaatg cgcattggtg
     1801 atctcaaaca acaattgatt gatttcttct tccatttctt ctttcttcaa aggggctctt
     1861 tccatgtata aactaggctc tcctggctca tctacccttg ttaagcgcat atgaagaatc
     1921 tttacttgag ggtttccttg ataaacctct cttttaccgt ttagaaaaac gactgtgccg
     1981 gctgtaaatt tattgatttc ctcctccgaa gcatcccagt atttaccatc gatagtacct
     2041 gaagtatctt ggaaagtaaa agcaataaat ttttttccgt ttttagcgat tcttacatct
     2101 gcacttttga tcaggacaaa ttgttcaaac tgctcatcga ttactaaatc acgaattttt
     2161 ttcattattt gctccttcta attgttttac gatttggttc atctcatggt aatagcttac
     2221 catttcttta tcagaagaaa aacaaattat ttgatacttt tctccaaatt tagctaaaag
     2281 ttcagctaat tgatactttc gctgatgatc ataatgaagc cagccatcat caatgatgat
     2341 cgggcatatc gaacggttag cttctaatga gaggtaagca aatctcatcg ccataatcag
     2401 ctgatctttc gtacctgtag ataactcata gatgggaaat tctgtatatc cgtctgttaa
     2461 agtcagtgta tcatttttca gtaatatctt ctgataattt ccttttgtta aaatacctaa
     2521 ataatcacct gctgctctta acaactgggg taattgtctt tcactcatct ctgttgaaag
     2581 atcacctaag aatgtcccgg cgatttcaag tgctccccat tcctccagta attcctgtaa
     2641 ttttgctttt tgccggctag cttgttgata aagttcgtcc aatgtgccgt ctctttgcaa
     2701 ctcttctact tgaagttgga gtcgttgccg attttcactt gcttcttttt cttcttgctg
     2761 gagttgggat tgcttttctt tcagttggcg cagtcttaca gataatttct cttttgtcag
     2821 ctcttcagga aatagtggag taagcatttc cgataattca ttgtatcttt tcatctgctg
     2881 ttcggcttgt ttggcttttt gtaaaaagac aggtacttcc gaaggataag ataagcctgc
     2941 ttgagtcaat tgttcgctgt attcttgtat ccatactttt tgtttctttt tcaattgatt
     3001 gatttcttgc ttcaataaag tattttgctg atagcttctg gcaaatttca tctgttccat
     3061 ctcttgtata aaacgggaaa gcaataccat tttttctgaa attgtcttat cttgtatcgg
     3121 taaccattct ttaacaaact caaatttttg gtcgatttgt cgtagttttt cttgtatttc
     3181 ctgttctctt ttatgtttcc gctcaaatac gccatataat ttctgataat ctatgacttc
     3241 ctgtccatat tctttcatga gtttgagcgt atccatcttt cctaagtgcc gatgatccgc
     3301 aaaacgatat acttcttgct gaagaatctg tttttcttct ccagttctct tttgtttttc
     3361 ttctgcttca gctaattgag ctaaggctag atcaagttgt cctagttttt cttgccacat
     3421 tccttttatc tcagagaaat catttttagt tttaattttt ggttgccttt tgtagaagaa
     3481 atagccaatc atgcctgcac caattagtaa aaggatcact ttcaagggca ttggtacaaa
     3541 tatacctgca atgattattg ctccgccagc catcagcata tacggtttag atgaagagac
     3601 tggtgctatc tctgactgat tttgtaataa tgctggatag gtctcttcca agtcattcac
     3661 ttctgcttca atcatctctt gctgttcttt agctatttgg agacgcacag aaatctggct
     3721 ttctagatca gcaatccttc tttcttcagc cagaagatgc ttccattctt cttcatataa
     3781 aaattcttcc ggtaacggat ttttctgcca atgccacctt tgttccaaaa tcttcaattc
     3841 tgaatttgtt tctctagtct caatttgtaa tctttgccat tcttcgttta attgttccgc
     3901 tgtaaattga agttgctgga gatcttttag ttcctgctcc tgctctaaat aaaaataata
     3961 tctggctgac tgctgatcca tcccgctatg cttttccagt gttgcttcta attcttccaa
     4021 acgctcattt gtttgctgat accgtttgga gaaagactcc agatctaaaa gaagttgctc
     4081 atcaggaaga cctgtagtgc tgtttttttt gagagattgc cattcttcat ataaagaaaa
     4141 attaagttct tgctgtctgg ctaatgaaaa ttgtttgttc gcctctttta attgtgccaa
     4201 taactgctgc tgttgcgact cgtattcctg ttcttgttgg atcaacttgc caaaataagc
     4261 ttcctgttct tgtttttgat gaattttttc ttgcagctct ttccattcat ttaatttttg
     4321 attgattagt aatttttggc cttttttctt gaataattgt tgtgctttct gataatattc
     4381 ttgccgcttt tggaatactt gagaactgcc cgtgatgcct aaagaaatca gcgcatcatg
     4441 tagctctttt tctctcaaat gatctagttc catcaactgc tcttgctgga aagtaaatac
     4501 ctgctggaat aactctttcg tcaaaggtgc gatcagtttt tctagtaact catcacttcc
     4561 cacttgatcc cccagccaca ccttcgattt tccgcgattt tgctgcttgt agcgctcgac
     4621 agcaaattca ccatatacag gatgtttaag ccatattttg ccgccatagg ctgagccgtc
     4681 ttttggtgta taatcctttt ttttacggcc ttttgaaggg aaaccaaata atattgcttg
     4741 aataaattga taaatcgttg attttcctgc ttcgtttccc ccatagatca gctgattgcc
     4801 tgaataaaag tcaaaacttt tttgacgcca ttttccaaaa gcagcaatct cgatccttaa
     4861 aatcttcatt cctgatcctc ctcaaataaa aaatcttgat taagcagctg cttggctttt
     4921 tcaagtgttt cttcttgata ttccggcagt tcgctcaata ttctagccgc ttcttgatga
     4981 ctatatactt catttaaaat ttgttggaaa tcttgaggtg tctgatagta ttgaaataac
     5041 tgttccatca atttagcaga agcagcaagt ggggtcttcg tataaaaagt attctctatg
     5101 agagaaatac gccaaataaa gaaatcattt atcctatctg agaattcctc taacaaatag
     5161 tctaatagct ctcctgattg tatccgttcc aaaacatctg tattgagatg gtcataatct
     5221 tttaatttta tctcaattaa actaaatcca tcctctactt ttgaaagctg ctgacgaatg
     5281 tgaacaatga cctcttttgt cgttcttgct ttcctcaatg atatttcctt tgtcttccaa
     5341 tagacctctg ctactttaat cggatgtatt tggcaacttc ctttacttaa ttcaactagt
     5401 agaatagatg tagaagcttg ttccttttta gtatgacctt gaggcgcacc tggataaaca
     5461 attgtttgct tttcatttaa gaccgttggc acatggatat gccctaaagc ccaataatcg
     5521 tatccctttt cttgcatctt tgagggttgg aaaggagcat agtttccttt ttgagagacg
     5581 ccaggttccc cgtggtacaa cccaatatgg taatctgtga gttctctaga aggaaattcc
     5641 atcactttgt ctttttgaat ccacggttga cgataactaa atccactgag agagacagtt
     5701 tctccacttt ttatcgttat tttttttgtc tctaccgttt cggatgtaaa taaatggaca
     5761 tttttaggaa aggagaacca ataccgctct ttttgataga aatcatgatt tccgaatatt
     5821 ataaatacag gtatatggtt cttttctaac cgtttcatct gttctgaaaa atgtttctgt
     5881 atttttaatg aaggacgatt ttgatgaaaa ttatctcctg caagtaaaac aaaatcaacg
     5941 ttatttatta ttgcctgttc aatgatattt gataaaacgg tcagattcgt cttcaatagt
     6001 ttttcctgta cagttttatc aagagttgtc agtccttcaa atgaccgatc catatgtaaa
     6061 tctgccgcat ggataaattt caccattaaa aatttcctcc tttttttctt ttatcatacc
     6121 attttcaatg ttttttttca tcaataaccg aaaatttgtt cgtattatgc aaagaaatta
     6181 cgataaatgg ccgacttcaa aaaataagtt tattcgaaaa aaagattgtg acatgatttt
     6241 tgtcacaatc ctaaatatct atgttatatt cgcttcgttt gacactgaag aagtactatt
     6301 tgtactggaa tttatgctcc atttttccgt atttagaatt ctatgttcta tactacctta
     6361 attagtttat atggtaagaa ttctttcaaa tgcttgagat actccgtctt tatcatttga
     6421 aacagtgaca taatcagctt ttttcatgat ttcttgatga ctatttccca ttgcaaaact
     6481 aatatctgca ctttcaaaca tagataagtc atttaaacta tctccgatcg cgattctcaa
     6541 aggtttcttt aaattgtaga ttgattccaa acgatcaaac accacacctt tagatacatt
     6601 ttgaggtacg atttcaatat ttacaggtgc cgattcagta acagacacat ttagcatctt
     6661 tttcaaatgg ttaaatatat tagctttcaa ttgacgatta ggaataaaaa taaaaaattt
     6721 gtaaatgctg atagcatcct ttt
//
LOCUS       DAHUWM010000146         6537 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHUWM010000146 DAHUWM010000000
VERSION     DAHUWM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.148"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            53..2728
                     /locus_tag="NK788_002101"
     CDS             53..2728
                     /locus_tag="NK788_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCH6770421.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2910..3149
                     /locus_tag="NK788_002102"
     CDS             2910..3149
                     /locus_tag="NK788_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770422.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3165..4538
                     /locus_tag="NK788_002103"
     CDS             3165..4538
                     /locus_tag="NK788_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770423.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4542..6176
                     /locus_tag="NK788_002104"
     CDS             4542..6176
                     /locus_tag="NK788_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770424.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6339..>6537
                     /locus_tag="NK788_002105"
     CDS             6339..>6537
                     /locus_tag="NK788_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770425.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2295 a         1062 c         1312 g         1868 t
ORIGIN      
        1 gaggtaatgt agtcatacaa ataaaaattt tttagaaagt gagtggtaat taatgaaaat
       61 gaatcacact tcaattcaga agcatttaca actgttaaat acactgggga tttcttatgt
      121 taatttgaga actttttcta cattaaacaa gaaaaccgtt tctcatattt tttctactgc
      181 agattctcaa gcaattatta actatttaga aaaacagaaa ttgtctgaag gtacagtaaa
      241 cataacttat aatcctatta aacaagaggt gattacacaa aaaaagcatt ctgtgagaga
      301 caatgatatt gaaaaaattc ggtttgtatt tattgatatt gaccctaaaa ggaaagaggg
      361 aagtgcaaca gatagcgaaa agaaaaaggc agaaaatgtt atggaacaag tagaacgtta
      421 tttgaaagaa aagagaatag aatcatttgt aaaagttgat agtgggaatg gctaccatat
      481 ttttcttccc ataaatgaac aaccaaataa tcacgagacg attcttacta ttcagaattt
      541 tcttcaatta cttcaccgaa gatttggagt agaagatgaa gttgatattg atacttctgt
      601 ttataatcct tcaagattat gcaaattagt tggtacacca gcggtcaaag gaaagtccac
      661 aaaagagcgt ccgcatcgtt tatctagttt cctttctatt cccgataaca tagatagaag
      721 taattgtttt aaaattcttg aacagatagt tgaagaaaat tccatgatgt caacctctag
      781 agtaaaaagc catccatctt ttagagaagt gttacctttt attcaagcag actataaaca
      841 atggttagct cactatcctg aattaactta taccataaaa gagggcgatc ataaaggagt
      901 taccttactt atatttgacg aatgtccatt aagaaaacac acgaataatt ctaatggttc
      961 ttgtttgtct gttacggctg ataataagat tcgatttcat tgtctgcatg cttcggataa
     1021 aaataaagat attcaagact ttgtaaaaaa atatccgttg ccaaaacctg taaatccttc
     1081 tgtagaatca ttagaatcca atcaagaata ttattttgac cagtttaaac tgaatcaaga
     1141 aggagtattt ttggtgaaag aaggtcaaga agaaaaagtt gcttgtccga tgtttattaa
     1201 gcaaatacgc aaaaatatcg aacaaaaagt cattacactt gttcttgcct attatgtcga
     1261 tcattgttgg gatgaaatag aaatagaagg tggatgttta caaactaata attttaaaca
     1321 attagcaaaa catggcttaa tcattaaagc tagaagagaa gctgaagtcg ttgacttttt
     1381 acagattcaa cgggagaatg ctccaatctt ttttgaacat gaatctcttg gctggaaaaa
     1441 agaaaatgaa gcaatgattt ttcagttaca agattctttt ggacgtgaaa ttccttctgt
     1501 attaactaaa aactcatgct accaattaac aactaatggt acagaagcgg aatttgacgc
     1561 attggttcat aagtatgttt taggaacgaa cttagaactt gcttggtcga ttggtattgc
     1621 ttctattgtg ttaggctatt taaacgttac gaaggctaat acttttttga gtttactaat
     1681 taacctacag ggcaagagca caacgggtaa aaccactacg ttacacttta ttgcctcttt
     1741 atttggaaat ccaactacgg ttcttagaaa tatgaatgct accaataaag ccattataaa
     1801 gctagcatca aacaatcagg gaggtgttcc actaatacta gatgagttag gagcagcaac
     1861 tcaaaataac tttagctcgt tgatctatca attagcttca ggagaagaaa ggctaagatt
     1921 agattctaat tgtcagctta tgccacaaga gactttttct gttttgaccc ttacctcttc
     1981 tgaagagcgt ttggaaaatt atttcaatga gaaattaact ggtttgaaag ttcgatatat
     2041 cgaattttct gatatcaagt ttacaaagga tgcgaatcac gcagagaaag ttaaaagtct
     2101 ttcttctaat acttttggtt ggggtataaa gttatttctt ttgaaactct ttgaacaagg
     2161 gattacaaaa attgaagcaa cttttgtcga agctcaaaaa aaactatggg ctgttttacc
     2221 tgaacattct ttgaagcaac gcattggtaa taatctagct ttgatttata gtggagccct
     2281 cctaattcaa gatatactgg gctatccttt acgattagag catatacaga cggtgcttat
     2341 taattcgtac gaacagttaa ttgtagatac tagtttagga agtgagcaaa cggtatttga
     2401 ccgagtgaaa acattgttgg tgatgaatag ccataaattt ttaacaaaca gtgacaacaa
     2461 acatacgccc agtacgattt ggggaaaagt atcaattcaa aaaaatgggg atattaaggt
     2521 aaatattttt cctatcccgt ttgaggactt gttaaggcga gaatttcaag taaaggatct
     2581 aacctatctt tttaggcaat tgcttgattc gggtaatatg tgtgcagaaa aagggagaag
     2641 aacgaaacgt attcacatga atcgtcaagt tcttccaaca tacgagattc tattaccttc
     2701 ttcactcaaa tcgtattttc gattgtaatt tagaataaac caaagtatga aagtgaggca
     2761 ccatgttatg gaaatatttc ttattgattt agcaaaacaa ctgattactt atctgtatga
     2821 tagcgagcaa acccaaacgg aagtaaatga agcttgatct atgcttcatt tacttcattc
     2881 aatcaaacat gaaagaaagg aaaattctaa tgactaaaaa taaaatgaca ctaaaagcag
     2941 aggttcttct gtatattcaa gaacattttt caaatcaagc cttttttaca aaaccaattt
     3001 acttggattt cgaaataaga ggggtatctg ctggatctat cgggggtact cttcaagcgt
     3061 tgaagaacga aggatattta gaaaatcact ttgtgcaacg ttcgttcaat agaagagtcg
     3121 tgaaaaaatg gtatttagtt cattcataga gaggggaatc aagcatgcgt gtggcttgtt
     3181 atgatcgaac gtctgtttat gaagaagcga aaattagtaa tgacgctcaa tatgatgcag
     3241 tgaaagagta tgttttagag aaatatgaac aaattcccca acgattttca gacaaaggag
     3301 aatcagggga aaaagcggag cgtccagctt ttcgacagct tagagcttgg gtttgtgcgt
     3361 cagaagaaga gggggtgatc tatgtttggc gttatgatcg attggcacgt caagccaaaa
     3421 tagccttaga atttattgag ttgtgccaac aacatcaggt tcaagtgtat tcgatttctg
     3481 aacctcttcc tgcttctttc tcacacgcgt caacgaataa actatttgtg caattattgt
     3541 tcacttttgc agaacaacaa cgaaacgtca ttattgagaa tgttcgtgcc ggactttcct
     3601 ataaaaagca acaacggcaa tatttgtctt ctgcggtacc atatgggtat cgtttagtga
     3661 atgggaaaat ccaacaagaa gtacacgaag caaggattgt tcgctatatt tttcaacttt
     3721 atttgaccaa aaaatatggc tacaaaaagc tttgtcaacg attgacgcaa caaaagcttt
     3781 tcttccgcga acgtcctttt cagccctatc atatttatag tatcctaaaa aatccattgt
     3841 actatggcga aatcaaagga ggatctttgg ggaagtatct aggaaccttt gaaccgattc
     3901 tctcgaaagc aaccttttta caagcccaag agattcgcca atcaaggtgt acagctaaaa
     3961 aagatacgta tctgtactta ttacgtcaaa aaattcggtg tcccttctgt ggtcgtcatc
     4021 tgagttcgaa gtatcaatgg aatacaaaga agacgaagac gcttcattat tatcattgta
     4081 ccgaccgaag ctgccaagga atttttcttc gggcagaaag ggttgagtca gaagtattgg
     4141 aaaagataca ggcttttttt caatacgaag gcgtctatca agccttgatt gcccaattaa
     4201 cgcaacaatt agccgaaaag cgaaaagatg aaaaacgcaa agtaatccaa caaaagaaac
     4261 aagaagcaca atggattaag gcatttgaag agggcaattg ttcactggaa gaactcaagc
     4321 aacaactgca agctcttcaa aagccgatgt ctacttcttc gtttctttca ctccaacagt
     4381 gtgaagcaaa agtgaaagaa tggcttcaac taaaagacct ctctagtcga caattattat
     4441 gtgactatgt ttgtcggatt gaggtaacaa aggataaaac agtttgtggg atttacttcc
     4501 gacaactgac acaaaatatt ttagaaagga acgaatgatc tatggaaatg atacaaacca
     4561 aacgagtcgc attgtatatt cgcgtgagta cacaagaaca agctttagaa ggacatagta
     4621 ttcaagcaca agaacaaaat ggacgacagt ttgccgaacg gatgggctat gaagtggtag
     4681 aagtttatgc cgatgaagga atcagtggga aatccacaaa aaatcggttc gcctatcaac
     4741 gaatgatgaa cgatgctcgg aaccatcgtt ttgatctact agtgatttgg aaattgaccc
     4801 gtctcggacg caatatgtta gatgtcttac aaacagtcga ggaattattg cgatatgacg
     4861 taggattaca ttcgatctcg gaacaatttg acgtgaccac ttcgacaggt aaattgatgt
     4921 tacaactcct tgggagtttc ggtgaatttg aacgcaacca aattgcggag aatgttcaaa
     4981 tgtcaatgca aagcttagtg aaagataaaa agcgttatgc aggaggccga cgtttaggct
     5041 atgtcagtgg tattgacacg aatggtcaaa aacaattggt cattgaacca gaagaggcga
     5101 aaattgttcg tctgatctat gcaaaatatt taggcggagc agggtatcga gcgattgcca
     5161 attttttgaa tcgtcaagga tatcaaacag tcaaaaagaa tgctttttct accacggcag
     5221 tcaaagatat cttacgcaat aaaatttatg cagggtattt agaatatgcg cgctacatca
     5281 attgggaaac caaacgacgg aaggggaaga atccgagacc gattttagtg aaaggaaccc
     5341 atgaaccgat tatttcggaa gaagattatc aaaaagtaca agaacgcctt gctttagaag
     5401 gaaaacaacc caaatggaac catacaggtg aaaatgtatt gacaggcttg ttacgttgtc
     5461 cagaatgtgg cgcaccgatg gcagcgagta atgtcacaaa taccctaaag gatggcacaa
     5521 agaaacggat tcgatattat tcttgttcag tctttcggaa taaaggagca agtgtgtgtc
     5581 atgccaattc gattcgtgcc gaacaagcgg aaacatttgt cgccgaccga ttaaaggaga
     5641 tcgttcagct accagaagtt cttccccgat tggtggcagc gttaaatgaa gaaatagtgc
     5701 gacaaagcca accattggaa caagaactcg tggtgttact ggaacgaaag gaagaattga
     5761 agactaaaat tgaaaaatgg gaagctgctt tggaagatag tcctgaattg tttccgatgt
     5821 taaaagaccg cttagacgaa ttgactgaaa aacgacgaca gttgcacatt cgagaaaatg
     5881 aaatcttagg gatctttcaa caaggagagc cgattcaagt caaagatgtc caacggatat
     5941 tgactaattt ggatcgtttt cttgcccaga gtgagaagaa gcaaatcaaa gccttgtatc
     6001 gtacctttat agagaaaata acctttgatc ctaaccataa ggagatgttg gaaattacga
     6061 tgaaattcac gcatgccgtg gtacaacaat tgaatgagca atatcaaata gccgtatcca
     6121 aaacaaagga tacggctatt tttgtgttaa aaacaccctt tacgttaacg atctagtcta
     6181 ggttatatca aacaacgtta gaggggcgct aacgcttctc tagcgatttg aaatcgttag
     6241 agaagtagtt agggaggtcc ttatcataaa tagggatatt tattaatgaa tatttgaaat
     6301 ataattgtat ataagaatta aaaagagtgg ggatagatat gaaaaagatg ggtaagtgta
     6361 ttttatgtga aaaatacact gaacttacta aagaacatgt accacctaaa aaatcaggga
     6421 atactggagg taaaaagacc agaactggta atttagatga tttccttaag agcgatttta
     6481 caaaaggtga ttttccgaag gggattaaaa ggaaacctca aggaaatgtt tattata
//
LOCUS       DAHUWM010000147         6486 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHUWM010000147 DAHUWM010000000
VERSION     DAHUWM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.149"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..894)
                     /locus_tag="NK788_002106"
     CDS             complement(<1..894)
                     /locus_tag="NK788_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCH6770426.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALS"
     gene            1531..1617
                     /locus_tag="NK788_002107"
     CDS             1531..1617
                     /locus_tag="NK788_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCH6770427.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(1738..2427)
                     /locus_tag="NK788_002108"
     CDS             complement(1738..2427)
                     /locus_tag="NK788_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770428.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2434..2712)
                     /locus_tag="NK788_002109"
     CDS             complement(2434..2712)
                     /locus_tag="NK788_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770429.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(2672..3586)
                     /locus_tag="NK788_002110"
     CDS             complement(2672..3586)
                     /locus_tag="NK788_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCH6770430.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(3568..3948)
                     /locus_tag="NK788_002111"
     CDS             complement(3568..3948)
                     /locus_tag="NK788_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCH6770431.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(4398..4685)
                     /locus_tag="NK788_002112"
     CDS             complement(4398..4685)
                     /locus_tag="NK788_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCH6770432.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(4705..4929)
                     /locus_tag="NK788_002113"
     CDS             complement(4705..4929)
                     /locus_tag="NK788_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCH6770433.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            5061..6140
                     /locus_tag="NK788_002114"
     CDS             5061..6140
                     /locus_tag="NK788_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6770434.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         1752 a         1334 c         1232 g         2168 t
ORIGIN      
        1 cgaaagtgcg gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat
       61 ttgttggagg agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc
      121 gggttcttcg tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt
      181 ggttggtgca tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg
      241 atagccaata acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt
      301 gaagattgca gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg
      361 cttataactt ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa
      421 acctttggtt ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag
      481 ctcgttgaat tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag
      541 tgctttttcg taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa
      601 ttctgtaaac attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg
      661 taaagataag attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt
      721 tgctttgtag ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc
      781 tttttcacgc attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt
      841 tcttaaagga attgtattta attcatggtg gtattttaca atctcattag ccatataatc
      901 acctcaatcc aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca
      961 aacagacaaa atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc
     1021 ttcataaaca gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac
     1081 agcttacggt aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta
     1141 attttcccgc ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac
     1201 tgccaatcca aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac
     1261 attgtactgc gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt
     1321 tgagcggttc tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat
     1381 ctagctttaa agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga
     1441 tacttaaaaa caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga
     1501 cagtcttgcg actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt
     1561 atagaagttt gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat
     1621 caataaaggc gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt
     1681 cgtgtatcta atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca
     1741 attttgctgt tgctgatcta accattcctt gacacttttt agataattta atgccgtttc
     1801 ttttttcgtt ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta
     1861 ggtaactgat tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc
     1921 actgaataaa ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt
     1981 tttggttcgc tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata
     2041 aagacgagtg agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat
     2101 atcgttcatt tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc
     2161 ctttttcttt ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact
     2221 tcttgaaata cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa
     2281 tgtaaatata acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga
     2341 atatcttgtt tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat
     2401 gagtagatag ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc
     2461 taacagtgtc atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat
     2521 tacttttttc tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg
     2581 tgaatcaatt gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt
     2641 ctttatttca ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg
     2701 ctcctctttc aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag
     2761 tcgtaagagt tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag
     2821 ctcttcgtag ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat
     2881 gtaaaatcgt ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt
     2941 tgtggatcaa ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt
     3001 taagagaatc acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa
     3061 tcgcaaaccg aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag
     3121 acatggttcg tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac
     3181 aaatctcgtc atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt
     3241 tgttcccagt ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt
     3301 ctgtatgtgt gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc
     3361 ctagttgatt ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt
     3421 gtgcttgtac acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg
     3481 cataatcaat gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga
     3541 tcgctcggct acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc
     3601 cttttgcatg atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg
     3661 ttggtgtgtg ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc
     3721 gcgcgcgatt gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg
     3781 tgccttggct ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag
     3841 ctttgcaaat tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt
     3901 atatttttta gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct
     3961 ttcagtacgt cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa
     4021 gtaaagctca ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac
     4081 ctacgtattt ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc
     4141 ctccccccca ctctccacaa tacaaaaaag actagcaaat gctagccact ttttgtaaag
     4201 tttcgcttgt ttcaccttcg gtgaaattgt tgcaaatcaa taattaggta gagctacatt
     4261 tttttcgttg ttcaaagatt cttatgccat ttactattaa taaatctata aatatgataa
     4321 tttttcggct ctataattta tgggactgat gaatcagaat tgattcttca gtcccatttt
     4381 cattttattc caaagaatta actccaaata ccaatagaag cccatcctct atatacttga
     4441 gattccttac taacaatttc ttgtaattct attaaagatt taggtaatcc aaatttatgt
     4501 tttaaaatat atcttccaag aaaactactt aatttcatac taacttcacc aacacttttt
     4561 ccctcactga tttctttaga ataaagaaag agcttttctt ttaagtcagg atagtcatct
     4621 atagttgctt cattgtatac tttactaatt gcatttaaaa gtttttcttc tttagtaaaa
     4681 tccattataa taacctccct accactacct tctaggtgcc catggaccat gattaaccca
     4741 accattaaca ataatttttc caatttctcc tttagcttga ttccaatcta cccaacattt
     4801 ttctttgtta caataaagac catttccata gtaagtagct gcatcaactt ttattcctgt
     4861 accgatgcca gctagacaag ttcctaatat tcctagaata acacaatgtt ttaatacttt
     4921 ctttttcatg attgtttttc cttttcgttt ttttggcgga ttttataatt aacttagcca
     4981 tttggaaata aaaaaacgcc acgtgaattc catgttatat tgaagttacc acaactctca
     5041 atagacaagg atgaattcac atgacgcaaa accatgataa cacagtttca cgcaagggaa
     5101 aacacctaac ctactcagaa cgttgccaaa tcgccatcct gaaaaaggaa aactattcca
     5161 accgtcaggt tgcggatgtc ctgggctgcg tgccgcagac cataaacaac gaaatcaaac
     5221 gcgggaccgt tactcagctt aaccgccaga atcagaacgg gaaggtctac gactattatc
     5281 atgacgtcta cgacgccgat gccgggcagg cggcctacga gaagaaccgc ttgaactgcg
     5341 gcagacggcc cttatgggcc gacacggacg ccttcattga atgggcggac gacatgatgc
     5401 tgttggaaaa atggtccccg gacgtggtgg tgggctttgc cagaaaacac gaattgttcg
     5461 acccggccat tatccctggg accaccaccc tctatcagtg gatcgaccgt ggcatcatga
     5521 aaacgaagaa tatggacctg ctagaaaaac tgtcccgcaa accgaaatcg tcccgtcatg
     5581 gcaaccgccc gaataaacgg gtgctaggga aatccattga agagcgcccg gaggcggttg
     5641 gtacccggaa aaccttcggc cattgggaag tcgacacggt cgtaggaaac aagaccaagt
     5701 cggatgccgt gctgctgacg ttggtggaac ggcagacacg cttcgaggtc attctgaagc
     5761 tggccgggaa ggatgccgaa tcggtcaacc aagcggtccg acagctgaga gaacgggctg
     5821 gtgcgtactt cggccagctg ttcaagacgg ttacctcgga caacggctcg gagttcgcgg
     5881 gactgcacga tgccttgcag gatgtggcaa acatctactt cagccacccc tatgcttcct
     5941 gggagcgcgg gacgagcgaa aaccaacaca agctgattcg ccgtttcatc cccaagggca
     6001 gcccaatcgg caacgtgagc gaaaaccaaa tcttgcggat tcagcgttgg atgaacgatt
     6061 acccgcgaaa gattttgggc tatgcaacgc cgcatgacac gttcgtccag gcatttaggc
     6121 aggaacggtt gacggcttga cacgtgggcc cttgacaacg agcctccctc ggcatggctt
     6181 gtgtctggaa agctggccgg ggccagccag tcgggagact accatgcctc agctcattgt
     6241 caagggccca ctcgattaga aaaattagac ggttggtggt aacgagcaac cgggtcacca
     6301 tgggtggcta acttaaactt gaaatttacg ttttcgtttt tttaaaattg cttgttatat
     6361 taaataatct tagtaatttt attatataac attttattat ataacaatga ttaacgtcat
     6421 aaaaagtagt tttgaattca taatcgttta aataaaatta taagatcagt tatttaaaca
     6481 gctagt
//
LOCUS       DAHUWM010000148         6477 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHUWM010000148 DAHUWM010000000
VERSION     DAHUWM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.150"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            3..734
                     /locus_tag="NK788_002115"
     CDS             3..734
                     /locus_tag="NK788_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6770435.1"
                     /translation="MIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKSTML
                     RCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKLAP
                     IKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEMLLF
                     DEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLEDS
                     RNVRDFFENPNEVRAQQFISKVINH"
     gene            748..1563
                     /locus_tag="NK788_002116"
     CDS             748..1563
                     /locus_tag="NK788_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCH6770436.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1576..2223
                     /locus_tag="NK788_002117"
     CDS             1576..2223
                     /locus_tag="NK788_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6770437.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2239..2895
                     /locus_tag="NK788_002118"
     CDS             2239..2895
                     /locus_tag="NK788_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6770438.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3057..3878
                     /gene="racE"
                     /locus_tag="NK788_002119"
     CDS             3057..3878
                     /gene="racE"
                     /locus_tag="NK788_002119"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCH6770439.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3881..5236
                     /gene="rph"
                     /locus_tag="NK788_002120"
     CDS             3881..5236
                     /gene="rph"
                     /locus_tag="NK788_002120"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCH6770440.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5237..5755
                     /locus_tag="NK788_002121"
     CDS             5237..5755
                     /locus_tag="NK788_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCH6770441.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5862..6362
                     /locus_tag="NK788_002122"
     CDS             5862..6362
                     /locus_tag="NK788_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCH6770442.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2129 a         1092 c         1433 g         1823 t
ORIGIN      
        1 caatgattaa atttgatcac gtggataaat attatggcaa gttccacgca ttaaaaaata
       61 tcaatctcga attcgagaaa ggagaagttg tcgttgtcat tggtccttct ggttcaggaa
      121 agagcacgat gcttcgctgt atcaatggat tagagacgat ctcttcaggt aagctgctga
      181 ttaatgatac tgacttgcat gataaaaaga caaaactgac agaagtccga aaaaacatcg
      241 gcatggtttt ccaacatttc aatttgtacc caaataaaac tgttctggaa aacatcaaat
      301 tagcaccaat caaagtgttg aagcaagacc aggcaactgc agtcaaaaac gcggagaaat
      361 ttttgaaaac tgtcaatatg ttagataaga aagattctta tccttccatg ttatccgggg
      421 gacaacagca acgggtggca atcgccagag gtttagcgat gaatcctgag atgctgctgt
      481 ttgatgaacc tacttctgct ttggatccgg aaatgattgg tgatgtatta gacgttatga
      541 aaaaattggc tcatgacggt atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca
      601 aagaagtggc ggatcgtgtt atcttcatgg ctgatggaga agtattggaa gacagcagaa
      661 atgtgagaga ctttttcgaa aatccaaatg aagtccgagc acagcaattt atcagtaaag
      721 ttatcaatca ttgataggag gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc
      781 cttttaggca ttgcttttct tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa
      841 gatattttag agcgaagcaa atctaccaat gaaatcatct ggggtgtcaa gtatgatact
      901 cgattatttg gaatgatgga tattgagagc agaactgtac aaggatttga tgtagatatt
      961 gcaaaagcaa tcactaaaaa gatccttggt gacaatggaa aaacagaatt tgttgaagtc
     1021 acttctaaaa ctcggattcc attattgaaa aacggaaata tcgatgcaat tattgcaacg
     1081 atgacgatca cagatgaacg aaaaaaacag gttgactttt cagatgtcta tttcgatgct
     1141 ggccaagctt tgttggtaaa gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct
     1201 tctacaacgg ttctagcagt caaaggttct acatcggctg cgaatatccg acagcatgca
     1261 ccagatgcaa aaattttaga attagaaaac tatgcagaag catttactgc tttacaatct
     1321 ggtcagggag acgcgatgac tacggataat gcgattttgt tagggattgc agatgaaaat
     1381 ccagagtatg agttagttgg tggaacgttt actaatgagc cttacgggat tgcgatcaac
     1441 aaagggcaag aaaacttctt gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat
     1501 ggcacatacg ataaaatcta tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag
     1561 taagggggag aggcaatgag cgaattgata caaacttatg gaccaatctt tgtcgacggg
     1621 ttcaagatca cattgttttc gagtgtctta gcacttgtgt ttagtttgat tatcggaacg
     1681 ttaatggcaa tcttccaatt atcaaagaat aaagtagtga gcgccttagc aaaggcatat
     1741 gtagaatttt ttagaaacat tccattatta atcatcgtga tgttttttta tgttgtcgta
     1801 ccgatttatt ggtttaatct agatggattc caagcaggta cgattggatt aacgatctat
     1861 acatctgctt ttattgctga aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa
     1921 acagaagcag gactatcttc tggttttacc tattcagaaa tcatgcgtta tattgtttta
     1981 ccgcaagcat tcaaaatcgt tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa
     2041 aactcatcta ttttagcaat cgtagctggt ttagatttga tgtatcaagg agacttgatc
     2101 gctagtgaaa cattcaatac cttcgatacg tatatcatcg ttgggttatt ttacttagtt
     2161 atcacgctac cgttatctta cttaatggtt tatcttgaga aaaaatgggc ggttcgttca
     2221 tagaaggagg ggaaatacat ggattttagc ggcgcttttt catggatgaa tatccgtttt
     2281 ttattggaag gattaaaggt tactgttgaa gtttcattgt tttcgatcat ttttagtttc
     2341 ttgatcggtg gtctgacagg tgtgattcgt tttgcgaata tccctttctt ttccaaaatc
     2401 cttggtgtag tgattgatat tatacggaat cttccattat tattgatcat tttcttcact
     2461 tactttgcct tacctcaaat cggcattcag atgaacatct tctggtccgc tgtcgcagca
     2521 ttaacgattt ttgagtcagc tatgttgtcg gaaattttcc gagctggact caatgctgtt
     2581 ccaaaaggac agatggaagc aggattatca acgggactga cttatgtaga atcgatgcga
     2641 acgatcgtgt taccgcaagc atttaaatca atgatcccgg caattgtgag ccaattgatt
     2701 tcactgatca aagatacttc attagcagta atcatttctt tacctgaact ttctcatcag
     2761 gcacgtatta tttatggaca gaacaccaat tatgtgcttc cgatgttcat catcatgaca
     2821 cttatgtact tcgtggtatg ttacgcgctt tctctgcttt ccggttactt ggaaaagaga
     2881 cggtacagtt attaatagat gattctttta gcggttgtgg caaactaaag tcacaaccgc
     2941 ttttcctctt tccattcgaa aaaaagagat ttcgattgtt ttaagcaagt aagagcactt
     3001 gaaaattcac gctaaaaagt acacaatagg gaggatagaa tttattgaaa aaaaggatga
     3061 tacgattgac agataatcgc cctatcggat ttattgattc aggtgtcggc ggcttgactg
     3121 tagtaaaaga agccctgaaa caattaccga atgaaaatat tttatttgta ggagacacag
     3181 cacgctgccc atatggccct agacccgcgg aacaggtaat ccagtatact tgggaaatga
     3241 cggattatct ggtggagcaa ggaatcaaga tgctggtgat cgcctgcaat accgcaactg
     3301 cggtggcttt agaagaaatc aaagctgctc tttctattcc agtcatcggt gtgatccttc
     3361 ccggtactag agcggcagta aaaaaaacac aaaataaaca agttggcatt atcggtacga
     3421 ttggtacggt aaaaagtcaa gcttatgaaa aagcactgaa agagaaagta ccagaattga
     3481 ctgtgacaag tcttgcttgt cccaaatttg tttcagttgt cgaaagtaat gaataccatt
     3541 catcggtggc gaaaaaaatt gtggcagaaa cattagctcc tttaaccact aaaaaaatcg
     3601 atacattgat tttgggatgc acccattatc cattattacg ccccatcatt caaaatgtaa
     3661 tgggagaaaa tgttcaactg atcgattctg gagcagaaac agtaggtgaa gtatctatgc
     3721 tgttagatta tttcaatttg agcaattcac cgcaaaatgg tcggacatta tgccagtttt
     3781 atacaactgg ctctgccaaa cttttcgagg aaatagctga agactggctt ggaatcggac
     3841 acttaaatgt agaacatatc gaattgggag gaaaataagg atgcgtcatg acgggagaaa
     3901 agcccaagag cttcgcaaac tggagcttca aacaaatgtt ttcaaatatc cagaaggatc
     3961 ggtagtgatc cgttttggtg atacaacagt tatttgttct gcaacaatag aaaattctgt
     4021 tccccctttt ttgagagaaa caggaacagg atgggtgaca gcagaatata gtatgctgcc
     4081 tcgagcaacg agtacgagaa atcgtagaga aagcagcaaa gggaaacttt ctggtagaac
     4141 aatggaaatc cagcgtctta ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt
     4201 aggagaacgt agtatcatcg tagactgcga tgtgatccaa gcggatggcg gtactagaac
     4261 agcaagtatc actggggcat ttgtggcatt gaaattagcc attgaaaaac ttttaagaga
     4321 aaaagaattg tcagaagatc caataaaaga acatcttgct gcagttagcg taggaatctt
     4381 accagatggg acttgcgtca cagatttgga ttaccaagaa gattccgcag cacttgtaga
     4441 catgaatctt gtaatgacag aatctggaaa attcgtggag attcaaggaa ctggagaaga
     4501 agcaacgttt gatggtgaac agttgaatga aatgctcttt tttggaaaaa acgcgattga
     4561 agatttgatc aaagagcaaa aacatgcttt gctaacagag tttgctcaaa atgatgagcg
     4621 gatagaagaa acaaaaacaa tcatcattgc gacaagaaat ccaggaaaag cagaagagtt
     4681 tcgaaatatg ttcaaagaag cgggatatca tgtaaaaaca ttacttgact atcctgaatt
     4741 gccagatgtg gaagaaactg gcagcacatt tgaagaaaat gcccgattaa aagcagaaac
     4801 gattgcacaa ttgcttgatc agccagtctt agcagatgat tcaggtttga aagtggatgc
     4861 tttaggcggt atgccaggaa tctattctgc acgttttgca ggggaacaaa aaagtgatgc
     4921 ggggaataac gccaaattat tatatgaatt gacagatgtg ccggatgaaa aaagaacagc
     4981 acaattccat tgtacgttag tattcgcggc acccaaaaaa gacagccttg tagtagaagc
     5041 ggaatggccc ggtcgagtag cccgtatacc atctggcgaa aatggctttg ggtatgatcc
     5101 tttatttatc ccagaaggta agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa
     5161 caagatcagt catcgggctc aagcaatgaa aaaattgagt gccgagtgga aacaatggct
     5221 ggaaggagag agatagatgc ggtatttagt agtaagtgat aatcatggag acagagatat
     5281 cctcgttgac ttagtagatc gttatcgttc tgaagtggat atctttttcc attgtggtga
     5341 ttcggaatta gaagcttctg acccattatg ggatacattc caagtggttc aaggtaattg
     5401 tgattatgga ccaggattcg aacagaaaaa agtgatccaa actggtcagg atacagtatt
     5461 catgactcac ggtcatttat cgaatgtccg atttggtttg acacagttag caatcgaagc
     5521 tcgagctgca actgccgata tggtgttttt tggtcatacc catcaaatag gctgtgagat
     5581 ggatcagaaa atcctgtttt tgaatcctgg tagcatttct cagcctagag gcccgatcca
     5641 aatcccatca tatgctctta tcgaaagttt agatgaccaa atttctgttc aatactataa
     5701 ccgtgcccat caggcagttg aagagttggc cttttctttt tcgagagagc gctgaataaa
     5761 aaaactctaa tttttaataa aaataagctt tattttatga attttgcatc cggtttcggt
     5821 aaaatagtag tgttgaatga aatagaaatg gaggccatca gatgattgga cctatagtaa
     5881 gagaaatgct gctggaaaat caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg
     5941 ttatgtatca tcatccattg tcccatggtt tacttgtgtt atcgaaggtc ggttattcca
     6001 aaattcctgt attaggaaaa gatgatcact ttgtaggact agtaagttta tcagatgtgg
     6061 tcaataaaat gattgacttg caaacaatca gcatggaccc gttagaagga ctaacggttg
     6121 ctgatgtgac agaaacagat gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac
     6181 tccatttgtt agtcgattcg ccttttttac cggttgtcac agaagatcgg attttcaaag
     6241 gaatcattac aagaaaagag ctgcttaaag ccgtaaactt catggtgcat gaattagaac
     6301 gtcgtaatat attgtctccg aaaatcgaag aattaaaaga acaaatcgat atcgtgagct
     6361 aaagaacgag aaactaaaag ataatcaaaa aataagtaga aaaaacagcg taccagtttt
     6421 gactttatct tgaaaaagag gctgggacaa aagcgtttaa ctccaagaaa taagaag
//
LOCUS       DAHUWM010000149         6446 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHUWM010000149 DAHUWM010000000
VERSION     DAHUWM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.151"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(1..859)
                     /locus_tag="NK788_002123"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="NK788_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="NK788_002124"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="NK788_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCH6770443.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="NK788_002125"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="NK788_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="NK788_002126"
     CDS             complement(2603..3421)
                     /locus_tag="NK788_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6770444.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="NK788_002127"
     CDS             3582..4271
                     /locus_tag="NK788_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCH6770445.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="NK788_002128"
     CDS             4285..5787
                     /locus_tag="NK788_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6770446.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="NK788_002129"
     CDS             5800..6276
                     /locus_tag="NK788_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6770447.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         2092 a         1011 c         1168 g         2175 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccat
//
LOCUS       DAHUWM010000150         6385 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHUWM010000150 DAHUWM010000000
VERSION     DAHUWM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.152"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(231..907)
                     /gene="nth"
                     /locus_tag="NK788_002130"
                     /pseudo
     CDS             complement(231..907)
                     /gene="nth"
                     /locus_tag="NK788_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(914..1612)
                     /locus_tag="NK788_002131"
     CDS             complement(914..1612)
                     /locus_tag="NK788_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCH6770448.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1880..3064
                     /locus_tag="NK788_002132"
     CDS             1880..3064
                     /locus_tag="NK788_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCH6770449.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3070..3513)
                     /locus_tag="NK788_002133"
     CDS             complement(3070..3513)
                     /locus_tag="NK788_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCH6770450.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3739..4026)
                     /locus_tag="NK788_002134"
     CDS             complement(3739..4026)
                     /locus_tag="NK788_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770451.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4197..5549)
                     /locus_tag="NK788_002135"
     CDS             complement(4197..5549)
                     /locus_tag="NK788_002135"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCH6770452.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5822..6268)
                     /locus_tag="NK788_002136"
     CDS             complement(5822..6268)
                     /locus_tag="NK788_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCH6770453.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1731 a         1269 c         1060 g         2325 t
ORIGIN      
        1 gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt
       61 cccaataaaa ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta
      121 gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc
      181 cataataaca agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt
      241 ggtttctttt ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt
      301 gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa
      361 aaatcagtgt atgatgtgtc tttacccata attcttgtgg tactttccgc atcagtgttt
      421 cttctacttc catgaccgta gcatctagtt tgcagatcct caggcgtttt gagacacgtt
      481 ccacgtgggt atctactgcg attgctggta tcccgaatgc atcccccaat acgacatttg
      541 ctgtctttct gcctacacca ggaagagaca tcaattcttc tcttgacgtt ggcacttgcc
      601 catcgaaacg ttcaatcagc tgctgtgcac acgctttgat gttttttgct ttatttcgat
      661 acaaaccaat cgtttttatt ttaagaatga tttcatcgat tgaagcttct gctaacgcat
      721 cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt attcactgat acatctgtcg
      781 cctgggcact taagatcact gcaatcaata actcaaatgg attattgtgc ttgagttctc
      841 catgtgcttc gggaaacatt ccatacatcg tttctagagc ttccattgtt ttttgtttgc
      901 ttaacatatt ccgctactcc tccaaccaat ttttcagtga gactttcggc aattcttctt
      961 gttgagctga ctgttctact tctctttgaa gaagctgctg ttttcttcgt ttttggtctt
     1021 cttccacttg ttgttttgta cgtagatttt ttctttccca cgatagcaaa atgcggtcaa
     1081 cgtaattaaa actataagcc tgatttaata cggcttcccg cagtgctagc ttcaaaattt
     1141 ccggctgata atggtcttct tctagccatt gtccgatccg ctggaactcg atcgaagaaa
     1201 gcgggcgtcc aaattcttgt tcaaataatt gatacactga ctggatttct ttttcttgtg
     1261 ttttttcttc gtattttttt tcttgtgttt ttaaatactc acctaataga tcatagatgg
     1321 ggtaaagatt atatcgatct gcttttttac cgttgacaat cgtcgtttct atcttgataa
     1381 atccatttgt tacgagacga tccagtattt gaaaaatctg atcttgtttg attcccatat
     1441 ccatagcaat catctgcaaa tcagggaaag tatctccttc aagctgtgcc atatgcaact
     1501 gtagaacaaa taaaaattct tcagattgta gaccgatcct atgataattt ttaagtaaaa
     1561 gattagatat gactgtttgt ccagcgctta agtaattttc taaattcaac atgatttctc
     1621 ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag caagaacagg tttcttttct
     1681 atttacttca ctgatcggtc agatttacaa tgtcgtctac ttgatttttg tttgttcatg
     1741 tttttgatgc gtatcttcac ttttcggaat aaataaatta agtagatttc gctgttcctt
     1801 agaatgttga caaggaaaca aaattactat acactatttt tgtttccaag ttaacattat
     1861 tgcataaagg agtttttaca tgtacagttt cacaaataaa cattctaaag tttttggata
     1921 cgtctctgtc ttattcactg gattaggatt tactatcgtc agtcctgtac ttccgttttt
     1981 aacgctgcct tattctcact ccatacatca gcaagctttc tatatcactt tgctcatgtc
     2041 tgtttatgct ctagctgcct tcttatctgc ccccattctc ggtagcttaa gtgatcattt
     2101 tgggcgtcat ccgatcctta ttatcagttt attaggatca agtatcggat atttgctttt
     2161 tggtttagga aattcgatat ggatgttgtt ccttggtcgt atcattgaag gattgacggc
     2221 aggagaaatc tccaccttat atgcttactt tgccgacatc acggaaccta atgaaagaac
     2281 caaagttttt ggatggatgg gtgctttagt cggtatagga acaacgctgg gtcctattat
     2341 agggggtcta ttagctgagt tggggaattc tgttcctata tttattggag cactctttac
     2401 tttcttaaat gccgtttatg gctacacatt tatgctagaa agccttccaa taaaaaaacg
     2461 ttcagtagat ttaagtttct cccatgtaag accttttcac caattacaac aattattcaa
     2521 gatttcatcc gttattcctt tattgacagc aggttttgct gtttggttgg cagctggctc
     2581 tcttcaatct atcttttctc aattttcgat tgacgccttt cagtggaagg ctgggttggt
     2641 tggtttaagc ttctctttga ttggaatctt ggatactgtc tcgcaattgt tcattatgcc
     2701 tcgattgctc aaaaaatttt cagaacagca gatcacaaga atagggatgt tcagtgaaat
     2761 cctcgcctat ctattcatca cattatctgg aattcttcta ctgccgattt tgtttttatt
     2821 tgggattatc tgttatggtt tcggtgattc tatttttact cctgtattca atggccaact
     2881 atccaattct gtctcagata accagcaagg tcttgtaatg ggcggtaccc aaagtataca
     2941 atctctatcg cgcgtcatcg gcccgctaat cgcaggacag ctttatgctg cagcaccatt
     3001 tcttccgacg gtattcggat ttttgcttat gatttttgcc tattatcact acaataaaaa
     3061 atagtagctt catttgctac ttcgtataaa ttctctgtat cgaccgataa aacgaaatac
     3121 tgtgtcaaat tcttcttttg acatattttc aaatacagta gcatctcgct gtacccattt
     3181 ttgatggagt tgctcgtgtc tattccttat tttttgtccg attggcgtca attgaaaata
     3241 gatttctttt ttattctctt cgctttgata agtgtttatg atccctctag ctatcagttt
     3301 ttttgttagc ttactgatgg ctcctctagt catgtaacta gcattcgcta attttgtcac
     3361 gttaggatca gggattttcc cgatatgttc aatcacttct agttcattta gagtaaatcc
     3421 tgcaaattct ttttccattt ctggtttatt acgatgaata atctcattag ctaaatccag
     3481 atattcttct attagcagtt gtcttttatc catttctaca ccttcttttt ctttcattat
     3541 attgtttttt tgtttcctat tcaacaaaga taaaaatatt ttgtaggaaa acccaatctg
     3601 aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc cctttttctg attaaaagat
     3661 agtaaagaga cagtcatcgg taattgtgat gattttttgt catcacttaa ggatgagcgg
     3721 ccttttcaaa aactgtcttt aaagatcatc atcagtttct agcacatatg gctgaccttc
     3781 atatgtgtac tctgtattca atgtgccgtc attttcatct gagtagatgc gtctactgaa
     3841 aggaaaacga tgagaaacat tatctttggc gtcatttgct ggcatcgtgt aatattcctc
     3901 ttgcgtttca ttcaactgag agagtacttg atctgccaac tcatcaatat tgatcgttaa
     3961 tttcttcgaa gcacttttat aatcttctaa aaatgcatct tgttggacaa aatattcttt
     4021 attcatcttc tttctcacct ttcttttcaa aatcaaataa agctgcttgt tcttttatgt
     4081 aacagcttta tctttgctgc ttattgtgac aaaaaattcg aagaaagaca actaattgta
     4141 cttttcacaa aaaaagacag agggtcgtga caaaattgtg tcacaaccct ttttctttat
     4201 tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta ggttcaagta attccatggg
     4261 cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga gatcttctac agtcatttta
     4321 ttttgaacag ctaaagacat ggtatttgct gattgtgtaa tgtcgtattt ggacatgaac
     4381 tgagcaccta cgatacgatt cgttcctttt tcatagacta gctccatcat tactttttca
     4441 gtagttggca tgaactctgg acgataatta tcttcaaata cagtcgcttg gacttctaga
     4501 tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac cgatcgtcca gccaaatagg
     4561 taaagtcctg aagttccttg agtaccacga tacgccattt tatgatctgt caagttattt
     4621 cccacaagca aaccttgacg aacggcattt gttgccaaag gaatatagtt ttgcgtgcca
     4681 cttggattgt agttgactac tgcagagtct ccagctgcaa aaatgtctgg attacttgtc
     4741 tgcatgtatt cattcacttt gattgccccg tttggcagtg tatctacttt tccttcgatc
     4801 agttttgtat ttggacggaa gcccacacat aaaatgacca tatctgtatc aaatgtatct
     4861 gttggtgtag ctacttgttt cacttttcct tcttcatctg tgataaattc agtaacattt
     4921 tcgcctagcg caagagtaac accgcggtct gtcaattctt tttctaagat atctgtgaat
     4981 ggtttatcta gatatttatt caagatgcga tctaatccat caatcaatgt cacttgtttt
     5041 ccagattcga caaaagcttc tactaattca ataccgatat atccaccacc aacaattact
     5101 acacgttttg cttctttcgc ttgtgcaata atttcatttg cttgattgta gtttttgcat
     5161 aggagtacat ttttcgcatc gattccacta atcggtggga taataggcca tgaaccagtt
     5221 gtcatcacta atttatcata agaaaccgtt tctgtttctc ctgttttcag atcttttgcc
     5281 gttactgttt tattttctgt atcgacgtct gttacatcgt gttccatttt aactgttgct
     5341 cctaatgaag ataattcttc aggatttgag tagaacaagc ctgctgggtc ctttactact
     5401 ccgccgacat aaagtgcgat accgcaagat aagaaagaaa tattgtcatt tctttcatat
     5461 actgtcactt ctgcttctgg atgatttttt aaaatacttt ttacagctgc agtacctgca
     5521 tgagtacagc caattactac tactttcatt tatggtccct ccgattaaca ttttgtcctt
     5581 cgacaaaact actataacag aaataaaacg gatacaacag tttaatgctt gtgaattcac
     5641 aaaggtttgt ttgtagtttc attccttttc aagcataaga acccaatatc tacaagattc
     5701 ttattttaat aatattcact tttatatcta agattgtgaa taaaagacaa aaaaataaaa
     5761 aagctagcgt tttttttaca cagctagctt tttttaggta taacacttgt agaaactgtt
     5821 tttacatttg cgcctttttt gctgactctt tacaatccgg acagagtcca tacaattcca
     5881 accgatgttc attaatctca aaatcagtca gtctgcttgc agccatttct atatcttcaa
     5941 gccctgggta atgaaaatca acaatcttac cgcagttttc acaaattaca tgataatgac
     6001 gctttgaact gaaatcaaat cggctagaag catcgccgta cttcatttct tgaacaaatc
     6061 caatcgaagt aaataagcga aggttattgt aaacagtcgc aacactcata tttggaaacc
     6121 gatgttcaag tgctttataa atctcatcag ccgttggatg ggaatgatgt tcaatcaaat
     6181 attctaaaac cgcgtaccgt tgaggtgtga ttcgaatgtt ctcttttttt aacttttcga
     6241 taacctcttc gactaactta ttttccatgt aaacacccca cgttgttatt ctcgtcttat
     6301 catactattt ttacatttta aaatccactt aacatgaaaa gaaaattcaa ttttattgta
     6361 ttttaatgaa aaaagacgta aaaca
//
LOCUS       DAHUWM010000151         6375 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHUWM010000151 DAHUWM010000000
VERSION     DAHUWM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.153"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            37..1059
                     /locus_tag="NK788_002137"
     CDS             37..1059
                     /locus_tag="NK788_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCH6770454.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1070..3274
                     /locus_tag="NK788_002138"
     CDS             1070..3274
                     /locus_tag="NK788_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCH6770455.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3295..3444
                     /locus_tag="NK788_002139"
     CDS             3295..3444
                     /locus_tag="NK788_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770456.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            3520..4113
                     /locus_tag="NK788_002140"
     CDS             3520..4113
                     /locus_tag="NK788_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCH6770457.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4129..5028
                     /locus_tag="NK788_002141"
     CDS             4129..5028
                     /locus_tag="NK788_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCH6770458.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5031..5516
                     /gene="ssb"
                     /locus_tag="NK788_002142"
     CDS             5031..5516
                     /gene="ssb"
                     /locus_tag="NK788_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCH6770459.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5882..6142
                     /locus_tag="NK788_002143"
     CDS             5882..6142
                     /locus_tag="NK788_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770460.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2314 a         1105 c         1313 g         1643 t
ORIGIN      
        1 cccgattggt tgattgatta tgcagaaaaa gaaggattga ttgccgaact aaagccaaaa
       61 catgaacggc aaaatttctt agttcgagac gatcgtttgg atcatgcggt cgccttttta
      121 tggaaagatc cacagacaaa agagacagta ggggcgagtt atcaaggaac gttcattgat
      181 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
      241 catggcttta acctaaagat tggtgatcct aaacagttaa aattttttga gagtagtatt
      301 gatttactta gctacgctgc actgaatcgt gatcagttaa acgatacgtg gttagtctct
      361 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
      421 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
      481 catattttct acgaaaaaga acaattaatg ggcgcagttg atccatttac gaatcagaaa
      541 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caagagaata caaagtcatt
      601 tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag ctattatggc catacacaag
      661 acagaaaaca atctgcaatt aacgaaccaa ctggtttccg ctcacaaagt caatgcgtct
      721 tttggtcagc agctttctgt taacgattca atagaagtca ttagtttgaa agatatttgt
      781 agagaagtcg caaaagaatt aaaagattgt gaacgctcga atggtacgta tgattttgat
      841 cgtttttatc aaggaaaagg ggatattaat tctcagattc ttttctctta taaagcagaa
      901 caatactaca aaggctataa aaatcatgaa catgaatttg tacctgaagt gaaaaaggat
      961 tggaacgatc aattgaagca tgagatccat caacaggaaa ttagaaagca aaaacgtgcg
     1021 atgttgtttc aacaaggcag acaacaagaa agggagtgag atcgtcacga tgaaaactgt
     1081 cattttagca gaaaaaccgt cacaagcgaa ggcctatgcc gatagctttt ctaaagccac
     1141 ccgaaaagac gggtattttg aaatccaaga tcgattgttt tcgggtgaaa ctgtgatcac
     1201 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaattggaa
     1261 acaatggtca ttagagcatt tgcctatttt ccctacacat tatcactatc atgttcctaa
     1321 agataagaaa aagcaattca acgtggtgaa acagcaactt caatcggccg acaccattat
     1381 tattgcgaca gatagcgatc gtgaagggga actgatcgct tggacgatca ttcaacaagc
     1441 aggcgctgat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
     1501 catttatcag ggattccagc aattacgaga cgcagaagag acctatccca aatttgaaga
     1561 agcacaagca cgccagatcg ctgattggtt gatcggcatg aacggcagcc cactgtatag
     1621 tttactcttg cagcaaaagg gcataccagg aagtttttcc ttaggcagag tccaaacccc
     1681 aacgctttac atgatttatc agctgcagga aaagattaga aacttccaaa aagagcctta
     1741 ttttgaaggg aaagcccaag taaccgctca aaatggcata tttgatgcga agcttgatcc
     1801 caatgaaacc caagcgacgc aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
     1861 agtaggaaag cagccaggca cgattcttga agtcgaaacc gagaaaaaaa gcgcggcaag
     1921 tccacgtctt ttttctctat ccagccttca gtctaaaatg aatcagctca tgaaagctag
     1981 tgccaaagat actttggaag ccatgcaagg gttgtatgaa gggaaatatc taagctatcc
     2041 acgaacagac accccttata ttacagaagg agagcatgcg tatctgttgg atcatttaga
     2101 tgaatacaag cattttttaa aggctgaatc attccctact cctattcata caccgaatag
     2161 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacagt
     2221 tatgacagaa gcaacgtatg aaaagctgac acctttacaa caagcgattt atgatcaagt
     2281 tttaaagacg acggtggcca tgtttgcgga aaaatatacc tatgaagaaa ctacgatttt
     2341 gacacaagtc cagcagcttc agctaaaggc agtcggaaaa gtaccagtag atctaggttg
     2401 gaaaaagcta tttggcaaag agagcgacag aaaagagaag gaagaagaac cgctacttcc
     2461 taaagtaaca aaaggagaaa cggtgacggt agatctacaa atctttgaaa aagaaaccaa
     2521 gccaccacaa ccttacacgg aaggtacact aatcaccgcc atgaagacag ctggtaaaac
     2581 cgtggatagt gaagaagcac aatccatatt gaaagaagtg gagggaattg gtacagaagc
     2641 gacacgagca aatattattg aaaccttgaa gcaaaaggaa tatatcaagg tagagaaaaa
     2701 taaacttgtg gtgacgaata aaggaatttt gctttgtcag gctgttgaaa aagaaccgct
     2761 gttaacaagt gccgaaatga ccgccaaatg ggaaagctat cttacaaaga ttggtgagca
     2821 aaaagggaca caagctactt ttctagcaaa tatccaaaaa tttgtttccc acttgttgga
     2881 agtcgtccca ggacaaatcc aagccactga ttttggagca acgttacaag aggtgaaagt
     2941 agccagtgaa aagcaagaag cggcgcgtca tttaggatct tgtccaaagt gccaggaaaa
     3001 agaagtcata ctttatcaga aggtggcgac ttgcacgtct gaagcctgtg attttaaatt
     3061 gtgggtaacg attgccaaaa agaaactcac agcgacacaa ctgaaagaga tcattcagaa
     3121 cggccgaaca agtcaaccag tcagaggact aaaaggtcag aaaggttcgt ttgaagcaac
     3181 gatcgtttta aaagaagact ttacaacagg atttgagttt tctgaaaaga agaagaccac
     3241 ctacaaaaga aaagtgagac gtaaaacgag ttagaattga cggagggaaa aacgatgaga
     3301 aatacaacgc ataaagaaca agaaatcgaa aatcaaaaag ccaaaatacg ttcagagcag
     3361 gagaaaatgc gtgccagcaa acgaagacta cagcaattga agaatcaaca acggattcaa
     3421 aaagaacttg aattagatcg ataaatcctc ctgttgttaa tattaaacta acatgttata
     3481 gttatgttag tttaatatta acattcgaga gggaaagcga tgaaagataa agtcaagtgc
     3541 atttttgaga aatacaatgg taatctatcc ttgaaggatg ctagaaatga agggttatca
     3601 cctatgacgt tagatcgttt agccgaaaag gacaaaatag aaaaaatagc atcaggtttt
     3661 tataccttaa aagatagttt tgtggatgaa ttatatctcg ctcaaagtat ctatactaga
     3721 ggaatcttct ctcacgagac agcgcttgat ctttttcaag taggaacata tatccctaaa
     3781 aaaattaata tgatgtttcc taaaggatac aatgtttcga aagagaattt agatcagtat
     3841 gccattcaac cgcattacac gaataaagaa aactttgaac tgggaatcac agaggcagaa
     3901 agcttttacg gcaataaaat tttcgtctat gatttagagc gaacgttatg tgatatgtgg
     3961 aatcccagat ataaagcaag cgtagaagta aaacaagaag cattaaaaga atatatgaca
     4021 agtaccagca gaaatacaaa taagctaaga agatacatga atattttaaa atcaccaaaa
     4081 gaaatgactt tgtatatgtt gccactttat tgagaggagt aaaggtgaat gacaccgaca
     4141 caatttaaag cgaaaacaag aaatgttgca gctgagaaac atttaaaccc acaaattgtt
     4201 caaagatatt atatgatgga aaaattatta gaatgtattg ttgaatcgcc ttatcatgat
     4261 gattttgtat taaaaggggg atttctgatc ggatcaaaat acgggttaga aaaccgttca
     4321 accgttgata ttgatacgac ttatcgaaat tcgaaactaa caaaagaaaa attagaaact
     4381 gttttttctg aattaacagc tgctcctaca aaagatggca ttcggtttga attaaagggc
     4441 ttaacagaga cgagggaagc cgattattac ccaggttttc aagcaaagtt tattgctttt
     4501 cttgaaaatg cacgtgtacc ttttaaatta gatattactt caggtgattc tattattcca
     4561 gcagcattga tatatgaaca taaattgatg tttgaagata aaacaattca tattccagct
     4621 tatcctacgg aacaaatttt agctgaaaaa ttacatgcca cgtttagttt cggaatagat
     4681 aattcacgta tgaaagattt ctatgatctg tatatgatcc ctaaactaga acgtatcgat
     4741 gagataaatt tgtataagag tgttcaagga acatttaagg aaagaaataa tcaatcagtg
     4801 tttaagaatt tgtataatca ggaaatgcct caattgatga agaatgaggg attgagggag
     4861 aaatggaaaa gatatcagca agaaaactat tttgccaaag atatttcttt cgaatctacc
     4921 ttaacttctg ttgattcgct gatgcaatca attgtcgagc aagaggaagt agaacgaaaa
     4981 caaaatttta agagatcaca acaaatgaat caagaaatgg agagataaag atgatcaata
     5041 atgtaacttt agtaggtcga ttaaccaaag atcccgattt acgttacacc gcaagtggca
     5101 ctgcagtgac cacttttacc ttagccgtta accgtaattt tactaatcaa agtggcaatc
     5161 gagaagctga ctttatcaat tgtgtgattt ggcgcaaacc agctgaaacg atagcgacat
     5221 tagctaaaaa aggcagcttg atcggcgtcg tcggacggat tcaaacgcgg acttatgaca
     5281 accagcaagg acagcgtgtc tatgtgactg aagttgtggc cgataatttc caactattag
     5341 aaagtaaagc agcgacagaa agccgtatgc acgctgatca atcgtccagc agtccaagcc
     5401 ctaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
     5461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttctaagtag
     5521 cattttctat tggttgcatt tgctacggtt tatgttataa tttagacaac aaaaagaatt
     5581 ttcaatctat aaccaaaaac cactagctgg cgggctagtg gctttctttt acgtttctat
     5641 tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc aagccatttg
     5701 cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc
     5761 aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta
     5821 tagcataatt gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt
     5881 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt
     5941 ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga
     6001 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     6061 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
     6121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
     6181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     6241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     6301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
     6361 tctcaccaaa aggtt
//
LOCUS       DAHUWM010000152         6366 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHUWM010000152 DAHUWM010000000
VERSION     DAHUWM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.154"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(12..362)
                     /locus_tag="NK788_002144"
     CDS             complement(12..362)
                     /locus_tag="NK788_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCH6770461.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(377..2104)
                     /locus_tag="NK788_002145"
     CDS             complement(377..2104)
                     /locus_tag="NK788_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCH6770462.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(2318..3244)
                     /gene="trxB"
                     /locus_tag="NK788_002146"
     CDS             complement(2318..3244)
                     /gene="trxB"
                     /locus_tag="NK788_002146"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCH6770463.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(3389..4822)
                     /locus_tag="NK788_002147"
     CDS             complement(3389..4822)
                     /locus_tag="NK788_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCH6770464.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(4822..6225)
                     /locus_tag="NK788_002148"
     CDS             complement(4822..6225)
                     /locus_tag="NK788_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCH6770465.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         1676 a         1481 c         1093 g         2116 t
ORIGIN      
        1 ttcacctatc gttattgatg ttccacatgt ttgagtgtct ggttcaaaat atccagtaca
       61 tgagaatcat caagactata caaaatcgct tttccctcgc gatgagactt gacgacatga
      121 ttttctctta ataaacgtag ttgatgcgat acagcagact gctccatttc cactgcttgg
      181 gtgattgctg tcacattttg ttccccgtct ttcagatata gcagaatttt aagacgagtg
      241 ggatcactca acattttgaa taattgactg actttctcta cttttgattt ttgcgtgtct
      301 ggcaatccat tcacctccaa cttgaaaaaa gactgacttc taaagtttac cactttttcc
      361 atcagtctca agttttttat tcttctgtca tctttttgat aatcgcttca taagaggcta
      421 ctttttcggc tgcgtcttct gtagagcttc cttttacacc aatataaaac ttgattttcg
      481 gctcagttcc tgaagggcgg atagcgatcc agccattttc ttccaatgta tacttcaata
      541 cgtcggatgg cggtgttgtt agtttttcta cgtttccttc acggtctgtt ctcgtcaatt
      601 ctttaaagtc ttctgtttga ataacttttg ctccggcaaa ttctgtcggt gcttctttac
      661 ggaatttttc catcaaagca gagattttcg cagaaccttc ttgtccgctc attgtcacag
      721 aaatcgtttt ttctgcgaaa tagccatagg tttcaaaaat cgtttgcaag ccgtcataca
      781 acgttttgcc ttgtttttta tagtaagctg ctacttctgc taataaaagc aatgcttgga
      841 tcgcgtcttt atcgcgaaca aaaggtttaa ctaaataacc ataactttct tcgaatccaa
      901 acataaaagt atgtgaatgg tcttcttcaa attgttggat tttttcagca atgaatttaa
      961 accctgtcaa cacattgaac atggtagcac cgtagtgttc cgcaattgct gtaggcaatt
     1021 cgctggaaac gattgatttc aataccgctg cgttttctgg taaagtgcct cttgattgat
     1081 gtgcttccaa aatatattga gcaaacaatg ctccgatttg gttcccagtc aatacttcat
     1141 aagaaccatc tggcaaacga accgctgcgc ctaaacggtc tgcatcagga tctgttgcaa
     1201 tcagcaagtc agctccttct ttttctccaa gtcgtatagc atattcaaaa gctgaatgtt
     1261 cttctggatt tggcgatttg accgtagtga aatctggatc tgccacagct tgttcgggaa
     1321 ctagtacgaa ttgttcgaat ccagcttgtt tcaacgcacg ttcgcccaac attttccctg
     1381 ttccatgaag tggcgtgtac acaagtttca aatctttccc cattgtttca accaattcac
     1441 gatcaatcgt cactgctttg atttcttcta gataagcagc gtccacttct tctccgatga
     1501 tcgtgatcaa cccgctatgt ttagcttctt cgtcagataa tacttcgact tttagcggat
     1561 tgctgacttc acggacatat ttcgtcaatg catctgcgtc tgcaggaggc atttgtccgc
     1621 catcttctcc gtagacttta tatccattgt aagccgctgg gttgtgagat gcggttacca
     1681 tgattcccgc aaacgtcttc aaatgacgaa ctgcaaaaga aagctctggt gttggacgca
     1741 gactttcgaa tacataagac gggatattgt gatgtgctag tgttttggct gcttccattg
     1801 caaattctgg tgactgatgg cgtgagtcat aagcgatagc gacacctcgg cgttttgttt
     1861 cttcaccttg tgtatccatg aaacgtgcta gtccttctgt tgcttgtctt accgtataga
     1921 tgttcattcg attgatccca ggtcctaaga ttccccgcat acctgcagta ccgaattcca
     1981 acggtgcata aaaagcatct tcaagttttt caggatcttc acccagatca tttaattgtt
     2041 tttttaagtt ttcttctaaa ttttcttcat gtacccattg ttcatacact tgttcccaag
     2101 acatgaaaat aacacctctt tctgaaatat ggttcttaat aagtatagca ttcacttatt
     2161 agatatgaaa gtatgaggac caaaaaacgc ttaaatcaag aaaggattct tttatcaaaa
     2221 ggtgttggac gtgttcgcat aaatcagatt cagtaaattt cacgcgaaaa aaaagagtaa
     2281 aagaacaatt gaacccgttc ttctactctt tttaaactta tgctgttgtt tcttctaaag
     2341 actccaagta tttatacact tgttgtccag caataccgcc gtcaccaaca gctgtagtga
     2401 tttgtcgcaa atctttttca cgaacatctc cgatggcaaa aacgcctggc atattcgttt
     2461 tcatttcgtt gtctgttgga atccagccag cttcgttcgt caaacctgct tctctaaaag
     2521 gtgcagttaa tggatccagt ccaacataga tgaatactcc gtctgctgct aaatcaaatt
     2581 cattgccggt tttgacatta cgtgctttca cacctgtcac taccatttcg tttccaacga
     2641 tttcgtcaac gactgtatcc cacataaagg aaattttttc atttgcaaat gcacggtctt
     2701 ggatgatctt ttgtgcacgt aattcatctc tacggtggac gatcactact tcagaagcaa
     2761 attgtgtcaa atagatggct tcttctactg ctgagtcccc acccccgatg actactagac
     2821 gtttgttacg gaaaaacgca ccatcacaga ctgcacagta agaaacgcct cgtccagcaa
     2881 attcttcctc gccaggtacg cctaatttac ggtggataca tcctgttgcg ataatgactg
     2941 tttttccttc gtaactttta tcttcacaga tgacttcttt atggctgccg tgatccttga
     3001 tatcttgaac gattccataa gcattttctg tcccaaatct tgagacactt tcatacatct
     3061 tataagctag atcggggccc atgatcgaat cgaatccagg ataattttcg atttctgccg
     3121 tattgttcat ttggccgcca ggggcacctt gttcgatcat caatacggat aagtttgaac
     3181 gtgaagcata taaagcagca gtcatcccag caggacctgc gccaataacg attacatcat
     3241 acatatgtag attctccttt acttaatcaa gtaagcatac tttacagcca atgcgaaaaa
     3301 aagtctattg ttctgcttgc gtttctcgaa tagctttatt atactcgaaa aatccgctga
     3361 aaaacaatag acttttataa atcattgatc aatcgttcga aagattttct ccatttgaat
     3421 caattacttt tttataccag ttgaatgatt ttttctttga acgggctaat gtgccatttc
     3481 cttggttatc acgatcgaca taaatgaatc cgtacctttt cttcatctca ccggtaccag
     3541 ctgatactaa atcaatacat ccccaagtcg tatagcctaa tagatcgact ccgtctagtt
     3601 ctactgcatc cttcatcgcc tggatatgcg ctgccaggta atcgattcgg taatcatctt
     3661 ccacataacc attttcatct ggcgtatcga ctgcacctaa gccgttttct acaataaaca
     3721 atggtttttg ataacgatca taaatatcat tcatcgttat tctcaagcct aatggatcaa
     3781 tttgccaccc ccattcactt gcttgcagat atggattttt gacagaagca aagatatttc
     3841 ctgcagtctg ttccaagagt tcgttgtcgg tagtcgaaac tcgggaagca taataagaaa
     3901 aagagataaa atcaacagta tgttctttca gcaatgcggc atcccctggt tccatttcga
     3961 tctgaatgcc atttcgctcc atttctttca atgcataagc gggatattct cctcgtgact
     4021 ggacatcaat gaagaaataa ttttctcgat ctgattttct agaagcccaa acatcttcag
     4081 gattgcaagt gtatggataa tatgctcctg ctgccagcat acagcctact ttgttctccg
     4141 gatctacttc atgagcaatc ttcgttgcga tggcacttgc aaccaactca tgatgtgccg
     4201 cttgatattt gatttgttct tcattttctc ctttttcaaa atacagccct gcgcccatga
     4261 atggtgcatg aaggatcata ttgatctcat tgaatgtcag ccaatatttg acaagtcctt
     4321 tatagcgatt gaagatgact cgacacagat tttcataaaa accaacgagt tctctggaac
     4381 gccacgcacc atattctttg atcaaatgca ttggacaatc aaaatgcgta atcgtcacaa
     4441 gtggctcaat tccgtgcttt tggcattctt taaaggcatc ctcataaaat ttcaagccag
     4501 cttcattcgg ctctttctca tcccccattg gaaaaatacg actccaggcg atggacattc
     4561 gataagtctt gaaccccatt tctgcaaaca aagcaatatc ttctttataa cgatgataca
     4621 tatcgattgc aatttttgct ggataaaaat gatcctcatc aaattcaaac atttttttct
     4681 ttccggtgat aacaggaaaa cggtcttttc cgattggcac aacatctacg tttgctagtc
     4741 cgcgtccacc ttcatcataa ccgccctcac attgattggc tgctgtagca ccgccccata
     4801 aaaaatcttt tctgaatccc attatacttc ctccattata tgtgtattac ctgataattc
     4861 tcttgccagt tcatcttgaa cttcgattcc ttcttctttt aatcggctga ttgtctcctt
     4921 gaattgcggt aaatactgct ggtgtgcaat aaacaactca tccatgatcc gttttgctgt
     4981 ctttcctgat cgtataagtg gattgatcgt aaacgcctgt aaagctgtcc cataatcacc
     5041 agtcacagct gcttcgatcg tcagcaattc catattcttc attacttgca accagcctct
     5101 ttcagcagca ggaagagaac caaacgctac atttctcgct ccttgtgcgc caacataggc
     5161 agtgacttct actacacaat ctgccggcag gtcaggaaca gctccatcat tctttgtaga
     5221 taccacgatc tgtgtttctt tatttccata gattgaagca atcgtttcac aggcagcgtc
     5281 tgaataataa gcgccacctc gtttttgcaa ttgttcaggt ttatggtcta attctggatc
     5341 tttgtataat tcaaatagct ctgcttctgt ttctttcact tgctgcgctc ttgtaccaat
     5401 cgtttgatat tcttctaaag catgttccag catttcttct tcacgatagt aatagcggtg
     5461 gtagccacaa gggatcatgt tcatttgttt taattgttct ttgaaaaacg ggatatcaaa
     5521 gatatttttc ggtaatccat tctcttcatc gtataaacga tcaatcaatt ctgccgtgcg
     5581 gtcatttcct ttattatctg ctactttgtg ccaatggaaa tgatttagac cggcaaactt
     5641 gtagatcagc tcttcttttt tcttgccaag cattgctggt tctgtcatca tcgccatcac
     5701 tggcacattg cataatccaa tcactttatc ccatttgcca tatctgataa ctgcttctgt
     5761 caccatccca cttgggttcg cgaaattgat cagccaagca tttggacata aacgcttcat
     5821 gtcctcaacg attgccaaaa cgactggaac ggtacgaaaa gctttgaata tcccgccagc
     5881 accatttgtt tcttgcccaa tcatcccata ataactaggg atccgctcgt ctttgattcg
     5941 tgcatctaat tgaccgacac gaaactgagt cgtaacaaaa tccgcatctt tcaatgcttc
     6001 ttcccgatcc agagttgcat agatcttgac ttcatacgga gaagcatccc acatacgctg
     6061 agacatctga ctaacgatct caagcttttc ttttccttct tctacatcta cgagccagat
     6121 ttctcgtatc ggtaattcct catatctttt gataaatcct tccatcaact cgggggtgta
     6181 actgctgcct ccaccaatcg tagcgatttt gattcctttt gccatgttct cactccttta
     6241 cttttttaca ctttcattat cttttttgta aacaaaacaa acaatagaaa tctgtttctt
     6301 tccagtaaca tttacaaaat ttactgtttt ctagtggatt tttgtaaata tcttgtcaaa
     6361 aaatgt
//
LOCUS       DAHUWM010000153         6198 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHUWM010000153 DAHUWM010000000
VERSION     DAHUWM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.156"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(12..272)
                     /locus_tag="NK788_002149"
     CDS             complement(12..272)
                     /locus_tag="NK788_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770466.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            347..550
                     /locus_tag="NK788_002150"
     CDS             347..550
                     /locus_tag="NK788_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCH6770467.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(691..1176)
                     /gene="ssb"
                     /locus_tag="NK788_002151"
     CDS             complement(691..1176)
                     /gene="ssb"
                     /locus_tag="NK788_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCH6770468.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1179..2078)
                     /locus_tag="NK788_002152"
     CDS             complement(1179..2078)
                     /locus_tag="NK788_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCH6770469.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2091..2684)
                     /locus_tag="NK788_002153"
     CDS             complement(2091..2684)
                     /locus_tag="NK788_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCH6770470.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2760..2909)
                     /locus_tag="NK788_002154"
     CDS             complement(2760..2909)
                     /locus_tag="NK788_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770471.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(2925..5129)
                     /locus_tag="NK788_002155"
     CDS             complement(2925..5129)
                     /locus_tag="NK788_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCH6770472.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(5140..6162)
                     /locus_tag="NK788_002156"
     CDS             complement(5140..6162)
                     /locus_tag="NK788_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCH6770473.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT         1567 a         1275 c         1061 g         2295 t
ORIGIN      
        1 cagctcttct tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc
       61 gattcaatga ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt
      121 ccatcatcca taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc
      181 gattcatgaa ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg
      241 attttatcaa gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa
      301 aaacagctaa aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt
      361 aaagtttgtg acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca
      421 aaatcttaca gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag
      481 tttcggttct ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca
      541 aaaaaaataa ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct
      601 ggtcaccgtc ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc
      661 cattataaca actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc
      721 gattgattgt ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc
      781 tacatcccgt tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc
      841 acggctttct gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt
      901 cacatacaca cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac
      961 gccgatcaaa ctgccctttt tagctaatgt cgccatcgtt tctgctggtt tgcgccaaat
     1021 cacacagtta ataaaatcag cttctcgatt gccgttttga ttcgtgaaat ttcggttgac
     1081 agctagggta aaagtcgcta ctgcagtacc actcgcggta taacgtaaat ctggatcttt
     1141 ggttaagcgt cctactaaag ttacattatt gatcatcttt atctctccat ttcttgattc
     1201 atttgttgtg atcttttaaa ctgccgtttt ctttcttgat tttcaatttt gatcacactt
     1261 ttcattaggt gatcaatttc gttgactgta tctgtaaatg aaatggaatt cgcaaacgtg
     1321 ttaactgagc gatatttttc ccactgcttt tccaaatagt catttcctct gatcttatcc
     1381 atttctcttt cataataaaa ggacaacggt tttgtatccc cacgttttgt aaatgtattt
     1441 cgaacacttt tgtataactc tttcgtatcg atattttcta gtttcggaat ggtaaataag
     1501 tcgtagtaat cttttgctct cgaattatct gtgccaaagc tgaacgtagc acttagcttt
     1561 tctgcaataa tttgttctgt tggataagct ggaatctcca cttttttatc ttcaaacatt
     1621 aattggtgat agtgtgtatc tacttcagga taaatagaat ctcctgttgt cacgtccatt
     1681 ttaaacggta cacgtacttt ctctaaatta gccaccaatt taatttgaaa tccaggataa
     1741 aaatcggctt ctcttgtttc cttcattcct tgaatagaga aaacaatgcc ttcttttgtg
     1801 ggttttctag ttaaatcatt aaaaatgctc ttcaattttt cttcagttaa tttgctattt
     1861 ctaaatgtcg tatcgatatc aattgttgtc cttctatcga ttccatattt tgatcctaat
     1921 aaaaagccgc ctttcaaaat gaagtcattt ttgtactttg attcagaaat ttgttcaatt
     1981 agacgatcca tcataaaatg ccgttgcact aactgtgcat ttaatcctgt actctttgct
     2041 aaatttcttg tttttgctgc aaattgtgtc ggtgtcatct ttttcctcca tcaatataag
     2101 ctttgcatgt ggtatttcat ttctttttct acatgtagtt ttgtcatgta atctcttaat
     2161 ttacttggat ctctcaatgg gtcttccatg tactctttta atgcttctaa tttcatttca
     2221 tattcaaatt catatctcgg attccaaata tcacaaagtg tacgctcttt atcatacata
     2281 gtaataggat ttccttgaaa ggaagttccc tctacttttc ctagttcata aacttctttc
     2341 ttcacgaaat gaaattgtaa ctgctgctct tttgtaattg aacgatcaac gttatagcca
     2401 tggggaaaag ttaagtgaat gaatttcggc atattcgtag aaagctggta aatatctaaa
     2461 gtcgtttcat gagagaagat tcccctggaa aatcgttgct gaagccaaaa taaatcatct
     2521 attccatgac catcctctac ataataacca cgatcaactt tgatcaattt tccttgattc
     2581 accaatcttt gtacagtggt tgctgcgatt ccaaatttcc tggcttcctt catagataaa
     2641 tttccatcgc gaatagccaa caattctttc agtttatctt gcatttactt cccctccctt
     2701 cgttacaaat acactacatc aaatatatca tagtagttta tttgtaacaa gattaacgtc
     2761 tatcgatcta attcgatttc tttttgaatg cgctgctggt tctttaattg ttgcagtctt
     2821 cttttatttg cacgtaattt ctcttgttct aaacgaattt ttgctttttg attttcgatt
     2881 tcttgttgtt tgtgtgttgt atttcgcata ggataacccc ttcattattt tgtagtgcgg
     2941 cgtgttcttt tcttatagtt cgtttttttc ttttcagaaa attcaaagcc tgttgtaaag
     3001 tcttctttta aaacgatcgt tgcttcaaac gaacctttct gaccttttag tcctctgact
     3061 gattggcttg ttcggccgtt ttgaatgatc tctttcagtt gtgtggctgt gagtttcttt
     3121 ttggcaatcg ttgtccataa tttaaaatca caggcttcag acgtacatgc cgccacattt
     3181 tgataaagca agacttcttg ttcccgacat tttggacaaa cacctaaatg acgcgttgct
     3241 tcctgttttt cactggctgc tttcacctct tgtaacgttg atccaaaatc tgttgactgg
     3301 atttgtcccg gtacgacttc caacaagtga gagacaaatt tttggatatt agctaagaaa
     3361 gtagcttgtg ttcctttctg ctcaccaatc tttaataagt aactttccca tttggcggtc
     3421 atttcggcac ttgttagcag cggctctttt tccacggctt ggcaaagtaa aatcccttta
     3481 tttgtcacca caagcttatt tttttctacc ttgatatatt ccttttgttt caaagtttca
     3541 ataatatttg ctcgtgttgc ttctgtaccg attccttcta cttctttcaa tatggattgt
     3601 gcttcttcgc tatccacggt ttttccagct gttttcatcg ctgtgatcaa cgtaccttcg
     3661 gtataaggtt gtggtggctt cgtttctttt tcaaggactt gtagatctac cgtcaccgct
     3721 tctccttttg tcactttagg aagtagcggt tcttcttcct tttcttttct gtcgctctct
     3781 ttgccaaaca gctttttcca acctagatct actggtactt ttccgactgt ctttagctga
     3841 agctgctgaa cttgcgtcaa gatcgttgtt tcttcatagg tatacttttc cgcaaacatg
     3901 gccaccgttg tttttaacac ttgttcataa atagcctgct gcagtggact taattgttca
     3961 aaagccgctg ccgtcatgac cgtctttgta ggaatgatcg cgtaatgttc ctggactttc
     4021 ttattattca catatcgact attcggtgta tgaataggag taggaatggc ttcggccttt
     4081 aaaaagtgtt tgtattcgtc taagtgatcc aacagatacg catactcccc ttctgtaata
     4141 taaggggtat ctgttcgtgg gtagcttaga tatttccctt catacaaccc ttgcatggct
     4201 tccaaagtat ctttggcact agctttcatg agctgattca ttttagattg aaggctggat
     4261 agagaaaaaa gacgtggact tgccgcactt tttttctcgg tttcaacttg gtggatcgta
     4321 cctggctgtt ttcccaattg aacccccttt tcttttaaat aatcttcaaa tgcttcttgc
     4381 gtcgcttggg tttcattcgg atcaagcttc gcatcaaatg cgccattttg agcgattact
     4441 tgggcttttc cttcaaaata tggttctttt tggaagtttc taatcttttc ctgtaattga
     4501 taaatcatgt aaagcgttgg ggtttggact ctgcctaagg aaaaacttcc tggtatgcct
     4561 ttttgctgca agagtaaact atacagcggg ctaccgttca tgccgatcaa ccaatcggcg
     4621 atctggcgtg cttgtgcttc ttcaaatttt gggtaggtct cttctgcatc tcgtaactgc
     4681 tggaatccct gataaatggc ttctttttct aaggagttga tccaaagtct tttaaatatc
     4741 tttccatgat cggcgcctgc ttgttgaatg atcgtccaag ctatcagttc cccttcacga
     4801 tcgctatctg tcgcaataat aatggtgtcg gccgattgaa gttgctgttt caccaccttg
     4861 aattgctttt tcttatcttt aggaacatga tagtgataat gtgtagggaa aataggcaaa
     4921 tgctctaatg accattgttt ccaattttca tcgtacatat cgggcgaatc taactcgact
     4981 aaatgaccaa aaccatacgt gatcacagtt tctcctgaaa acaatcgatc ttggatctca
     5041 aaatacccat cttttcgggt agctttagaa aagctatcgg cataagcctt cgcttgtgac
     5101 ggtttttctg ctaaaataac ggttttcatc atgacgattt cactcccttt cttgctgtct
     5161 accttgttga aacaacatgg cacgtttttg ctttctgatc tcttgttggt agatttcatg
     5221 cttcaattga tcattccaat ccttttttac ttcaggtaca aattcatgtt catgattttt
     5281 atagcctttg tagtattgtt ctgctttata agagaaaaga atctgagcat taatatcccc
     5341 tttttcttga taaaaacgat caaaatcgta cgtaccatcc acgcgttcac aagcttttaa
     5401 ttcttttgcg acttctctac aaatatcttt caaattaata gcttctattg aatcgttaac
     5461 agaaagctgc tgaccaaaag acgcatttac tttgtgagcg gaaaccaatt ggttcgttaa
     5521 ttgcagattg ttttctgtct tgtgtatggc cataatagct tctggtgtga tcttttcttc
     5581 ttttgcgact tcttcataaa tgactttgta ttcttttggc acttgccaat cattagcgat
     5641 tcctcgttca caacgtactt tctgattcgt aaatggatca actgcgccca ttaattgttc
     5701 tttttcgtag aaaatatgtc ctgcacggtc attatccaca caaatctcga tggattgagg
     5761 aaaggcttgt ttctttctca attcagatac tgcctcacca aaataatgac tgatcacatg
     5821 gtgttttaac ccttccatcg aaactaacca cgtatcgttt aactgatcac gatttagtgc
     5881 agcatagcta agtaaatcaa tactactctc aaaaaatttt aactgcttag ggtcaccgat
     5941 ctttaggtta aagccatgat tcgctgtcga atttttatct atatgcttat atgtgcctct
     6001 ctcaccaaaa cgttcataat caatgaacgt tccttgataa ctcgccccta ctgtctcatt
     6061 tgtctgtgga tctttccata aaaaggcgac cgcatgatct aaacgatcgt ctcgaactaa
     6121 gaaattttgc cgttcatgtt ttggctttag ttcggcaatc aacccttctt tttctgcata
     6181 atcaatcagc cactctgg
//
LOCUS       DAHUWM010000154         6104 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHUWM010000154 DAHUWM010000000
VERSION     DAHUWM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.157"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            163..1008
                     /locus_tag="NK788_002157"
     CDS             163..1008
                     /locus_tag="NK788_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCH6770474.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1199..2386
                     /locus_tag="NK788_002158"
     CDS             1199..2386
                     /locus_tag="NK788_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCH6770475.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2392..2748
                     /locus_tag="NK788_002159"
     CDS             2392..2748
                     /locus_tag="NK788_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCH6770476.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2750..3088
                     /locus_tag="NK788_002160"
     CDS             2750..3088
                     /locus_tag="NK788_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCH6770477.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3164..3420
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3483..4517
                     /locus_tag="NK788_002161"
     CDS             3483..4517
                     /locus_tag="NK788_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6770478.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4510..5196
                     /locus_tag="NK788_002162"
     CDS             4510..5196
                     /locus_tag="NK788_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770479.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5220..6068
                     /locus_tag="NK788_002163"
     CDS             5220..6068
                     /locus_tag="NK788_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCH6770480.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         1968 a         1103 c         1367 g         1666 t
ORIGIN      
        1 aatgtatgat tacgagcaag cagacagctg attgtcttca tctaagttac tagttggatc
       61 agcaactgtt ttgttgtaca tttctattat agatggtttt ttgtatattt aaatcaaata
      121 gtttttatag taattatcat aaaaagttat ggaagtggtg ccatgtttaa gttattaacg
      181 acttttcgag cagtatatga aacaagaaat ttttccagag cagcagaagt actgttttta
      241 tctcaaccag cagtctcgaa tcaaatcaaa cagttggagc gggaattgaa tatcgatctt
      301 tttcaacgta acggaagaaa agagatgctt ccaactaagc aagccgactt attatatgaa
      361 cggacactgg ttttgttaga agaatgggaa gaaactcagc aaaaattgct caatgaacaa
      421 aatgaagaag aagtatgtcg gatcgttgct tcccacacct ttgctgtcta tcttttgcct
      481 cagttgatga aacatctgat caaaaggttt cctaaagtgc attttgagat cgaaatcacg
      541 aattctcatg aatcattaga acaagtagcg aaacatgaat ccgattttgg ttttatcgaa
      601 aagccattag agacttccgg cgtacaaaga tactctttga tggaagacca gctagtcatc
      661 gctggggacc caaaaagcaa attgtggctt gtaagagaag caaactctgg tgtttttcat
      721 tacacgcagc gttattttga agagatgaat atccaagata agaagatgat cgtgaaaagt
      781 aacgatgtaa ttgttgcgtt gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc
      841 tctgttccag aaaatgtgcc aatgactccg ttgaatttcc atcgatattt ttatttcatc
      901 caacgaacca atctggtttc tgatcagctg ctagagattg ccgaagagat ccttcgatac
      961 aatgaaagca cagaaacttc tcacagcgag gagaatctca gctggtagat aattgcatgt
     1021 atcaaaaaaa gttctatgat acactaaaga taagcagtag gcttttgtac tgcatttgct
     1081 caagcaactg cacaaacgga aagttaattt ttcccaaatg tctatggaaa gactttccat
     1141 tatctaactg gaccgctata ataatcagca gtatggtttt taagaaaagg gatgaaacat
     1201 ggaaaaaaaa ttaaaagctt cgaacgataa tcgtattgat tatggcgtga ttttaccagt
     1261 ctttctgtta tgtttgattg gtctgctctc attatatgta gcacttagcc atgatccgaa
     1321 caatccagca gtcttgaaag gcgtagggat gcaagttgtc tggtatttag ttggagcggc
     1381 agcaatcgtt gtcatcatgc atttgaattc caaatggatc tggaaactga ctccttacct
     1441 ttatggtttg gggttagtgg taatgggact cttgctcaaa ttctatgatc agaatttagc
     1501 tgcttcaaca gggtctaaaa actggtttcg attcggtgcg tttactttcc agccagcaga
     1561 gttgatgaag attgcgtaca tcttgatgat ggcactgatc gtaactaaac ataatacgca
     1621 agtaaaagaa cggacgatga aatcggactt ttggctgatt gggaagctct tgatcgtgac
     1681 aataccggtt ttagcattga tcatggccca agatgacttt ggaacgatgc ttgtctttct
     1741 tgcgatcttt ggcgggattt tcctgatgtc tggaatctct tggcgcatca tcgcacctgt
     1801 agtaatcttg gcagtagtcg ttggagcagg aacgatcttc ttggttacga ctgaaggcgg
     1861 aagagacttg ctttataaag ttggattcaa atcttatcag tttgcacgta ttgattcatg
     1921 gctggaccct ttccatgata cgtcaggaat gagttatcag ccggcccaag gacttttggc
     1981 aattggtacc ggtggcttgt ttggtaaagg attcaacgta agcaatatct atgtgcctgt
     2041 tcgtgagtcc gatatgattt ttaccgttat cggtgaaaac ttcggcttta tcggtggtgc
     2101 attcgtcatt ttcttgtatt ttattttaat ctatcgtatg attcgggtgt gtttcgatac
     2161 gaataatgaa ttttacgcct atatcgcaag cggtttgatc atgatgctgc tgttccatgt
     2221 ctttgagaat attggagcaa atatcggatt acttccttta acagggatcc cattgccttt
     2281 cattagtcag ggaggatcct cgattttagg gaacatgatc ggtatcgggt tgatattatc
     2341 gatgagatac caaaatgaag cacctgcaag atcttcaagg aggtaagaaa attgtacacc
     2401 ttttactggt acccgaaatg ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat
     2461 ggcgttagtt atcaaaccgt tgatatgatc aaagaagtac cgaaggcctc aacgattgag
     2521 acttggctag aaacgagtca agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa
     2581 tatcgggaac ttggactgaa agatcaagta ggcgagttta cggcttcaaa agcgagccaa
     2641 gtcttatcga cagatggtat gctgatcaag cgaccactga tcttacgaga ggaccagctt
     2701 gttgcaattg gattcaatga aaaaatttat gaaggagaac tgatataaaa tggcaggcaa
     2761 gcatttgaag aaaaaggaca acctttggat cttatttaat ggaaaagaat actgtgttgg
     2821 tttgacaaat gaagcgcaag aagaacttgg agacatcacg tttgccaatc tgccaaaagc
     2881 aggacaatcg ctaaaaaaag atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa
     2941 tgaatttgcc agcccgctaa cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga
     3001 tatcaatatc ttgaatgatt cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt
     3061 tgatccaaaa gaatttgacg aattataaaa tcagaaaatc acttgaaaaa agttgacact
     3121 gattcttcca gttgctataa tcataagcaa tcctatatga aacgagaaag ctttgaaaag
     3181 aagagtacat gtcacggacg tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa
     3241 tgagatggaa gatggtctta gagcagagta agcgagcgta agcaaactta ctacgggagt
     3301 gcccgttaca gcgcgacagt atacggaaag aagctaagtc caagtactgg aagaggctgt
     3361 tattgcgaag taacggtaaa tcaaggtggt aacacggaag ttcaagcttt cgtccttttg
     3421 cttaaggcaa gagggcgaag gcttttttgt ttgaaaaatc ataagagaaa gatgtgaatg
     3481 gcatgtcatt aatccaacta acgaatgtca aaaagacatt caatgggaaa aacggaaaca
     3541 tccaagccgt caatgacgtt tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg
     3601 gctattctgg cgcaggaaaa agtacattag tccgtctcct gaatggactt gagcttccca
     3661 cgagcggcga agtgatcgta aacgatcaag atattacaaa attaaaaaac aaagaattac
     3721 gtacattccg aaagaaaatc ggtatgatct tccagcattt caacctgtta tggtcaagaa
     3781 ctgtacttga aaatatccag ctgccgctgg aattagctgg cgtaccaaag agcaaacgta
     3841 aagaacgggc agaagaactg ttgcgtttag tcgggttaga aggacgcgga caagcatatc
     3901 cttcacaact ttccggcgga cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg
     3961 atcctgagat cctgctttgt gatgaggcta ctagcgcatt ggacccgcaa acaacagaag
     4021 aagtattaga cttattgtta gcgatcaata agaaattgaa tttgaccatc gtcttgatca
     4081 cacatgaaat gaatgtgatc cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga
     4141 aagtcgtaga agaaggagac gtattgactg tcttcagaca accgaaaaaa gaagtaacca
     4201 aacgctttgt acaacaagac atcgaaccag aagctgattc gacagaacta ttagctgaac
     4261 tcatcaaaga aaatccaagc ggccgtttgg caactttgac ctttagcgaa agaaatgcca
     4321 acgagccagt catttcccaa gcgattcgac gctataacat cgatgtcaac gttgtgtatg
     4381 gaaagatcaa acaagtgaaa gaaggttcct ttggttcatt gactgtcttg atgactggtg
     4441 aaaccaaaga attagatcaa gcagaagtgt tcatcaaaga acaaggagta ggaattgagg
     4501 tgatccatcg tggataaaag cttttcagaa acatatctag attttaaaca aatcaacccg
     4561 gaaacgatga atcaggctat ccttgataca ctatatatga cattaatctc aatggtctta
     4621 gtgacgatca tcggattagt tttgggattg atcctttact ctattggacg caaaaacaaa
     4681 ccgtatgctc gaatcattta tggaatcgta tcgatcatca gtaatatttt ccggtcaacg
     4741 ccatttatga tcctgatggt tttgatcatt ccatttacta aagcaatcgt aggcactatg
     4801 ctaggtgcgc aagcagccat tccagctctt gttttatccg cagcaccatt ttatgcccgt
     4861 ctagtagaga ttgcttttcg tgaagtcagc cctggtgtac tggaagcagc agatgcaatg
     4921 ggagcaagtt actgggaaat catctggaag gtcttgatac ctgaaagtat tcccgcattg
     4981 atttctggat taacagttac aacgatctcg atgatcggat ttactgcgat ggccggcgcg
     5041 atcggcgcag gtggacttgg aggtcttgct tggcaggaag gctaccagcg tggtaatcta
     5101 acagtgacat tcgttgcgac tttaattatt ttactaatcg tatttatcgt tcaaggaatc
     5161 ggtgatttct taactaagaa aacagacaaa agataatagg atataggggg atgtaggata
     5221 tgaaaaagaa aatttttgga tttgcagcag cgcttttgct gactgtagga ttagcagcat
     5281 gcggtaacaa cagcgacagc aaagattcag caaataaagc agacgacacg acattgaaag
     5341 tcggcgcttc accgacaccg cacgcagaga ttttggaaca tgtgaaacca ttattgaaag
     5401 atgaaggaat cgatcttgaa atcgtcaaat tcgatgacta tgtattaccg aaccaatctt
     5461 tggaagaagg cgacattgat gccaattact tccagcatat tccttatttg aacaaagaaa
     5521 tcaaagaaaa aggatatgac tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct
     5581 tgtactctaa aaaagtcaaa gatgtttccg aattaaaaga tggtgcaaca atcattactt
     5641 caaattctga atcagactgg ggacgcatca tcacaattct acaagatgct ggcttagtca
     5701 aagtcaaaga tggagtagat ctggaaacag caacattcga tgatattgaa gaaaacccta
     5761 agaacttgaa gttcaaccat acgatcgacc catcactttt agcttctacg taccaaaatg
     5821 atgaaggtga cctagttgca atcaacgcaa acttcgctta cggcgcaggg ttgaatccat
     5881 tagaagatgc cgtattgctt gaaaaggaca actctcctta cgtgaatatt attgcgactc
     5941 gtaaaggtga tgaagacagt gacaaaatca aaaaactgat tgaagtgctt caccgcaaag
     6001 aagtccaaga atggatcaca gacaaatggg gcggctctgt taaaccagta aaggctgatg
     6061 caaaataagg tatggaacaa aaaagatgag aaataagtct gtaa
//
LOCUS       DAHUWM010000155         6091 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHUWM010000155 DAHUWM010000000
VERSION     DAHUWM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.158"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(394..975)
                     /locus_tag="NK788_002164"
     CDS             complement(394..975)
                     /locus_tag="NK788_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6770481.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1158..1784)
                     /locus_tag="NK788_002165"
     CDS             complement(1158..1784)
                     /locus_tag="NK788_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6770482.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1806..3137)
                     /locus_tag="NK788_002166"
     CDS             complement(1806..3137)
                     /locus_tag="NK788_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCH6770483.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3292..3693)
                     /locus_tag="NK788_002167"
     CDS             complement(3292..3693)
                     /locus_tag="NK788_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCH6770484.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3779..3985)
                     /locus_tag="NK788_002168"
     CDS             complement(3779..3985)
                     /locus_tag="NK788_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770485.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4080..5582)
                     /locus_tag="NK788_002169"
     CDS             complement(4080..5582)
                     /locus_tag="NK788_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCH6770486.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5600..6055)
                     /gene="argR"
                     /locus_tag="NK788_002170"
     CDS             complement(5600..6055)
                     /gene="argR"
                     /locus_tag="NK788_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCH6770487.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
BASE COUNT         1615 a         1356 c          994 g         2126 t
ORIGIN      
        1 cgactttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
       61 cccaaccaaa cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc
      121 agggcagctt tttcacaacc tctccactaa acggtgttca atcagctaac cttcggtatt
      181 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
      241 tcaaagcgct gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa
      301 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
      361 gaagctgacc acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa
      421 tctttctttg atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa
      481 caataagata cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc
      541 atcggacagt ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg
      601 attgactaat tctagtagca acagacggta ttcttcatcc actgtactca actcaataat
      661 caaattacct acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca
      721 gtccaacatt tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa
      781 caattctgtc tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt
      841 tgaagaaaag taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg
      901 gattgacgtt tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt
      961 ttcctttgtt ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta
     1021 aattcatcat tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag
     1081 ccctcactat aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga
     1141 caaaagcaat tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga
     1201 tctgcaataa agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca
     1261 attgcatctg tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa
     1321 tcgctacaca agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc
     1381 aattggagcg ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc
     1441 gtattttcat ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc
     1501 actgattcat attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct
     1561 gcaccgccaa attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt
     1621 ttgtatcctt tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat
     1681 ttttctaaga tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact
     1741 tgcgggtcag tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc
     1801 gttatttatt ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga
     1861 tctctcaata acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact
     1921 tcatgagaat cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact
     1981 aaaactttga atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc
     2041 atatacttga ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct
     2101 acattggata aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt
     2161 gttcgtttat tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata
     2221 taagtgaact gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta
     2281 cgaaggtatt catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct
     2341 aatccaagtg atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg
     2401 attgttactt cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct
     2461 acttgatcta cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa
     2521 atcatttgcg cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg
     2581 acaactttag atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc
     2641 ccgatgatca ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc
     2701 aagtagcgac ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg
     2761 atgaaaatcc gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga
     2821 tggttagaaa gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc
     2881 atatgataat ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct
     2941 gtaaagtcga ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg
     3001 ttgatacatg ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct
     3061 tttgaagcta acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt
     3121 tgatattttt ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata
     3181 tgataaccga taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga
     3241 caaaaagcgg tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat
     3301 cgttacttct acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc
     3361 atcttctgca attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac
     3421 tacaccgatc tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt
     3481 ggactctgct gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga
     3541 agcataacct gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg
     3601 agtgattttc atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg
     3661 gttgatcatt gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt
     3721 gctacttggc tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt
     3781 attcgaatac cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg
     3841 tgaacaacag cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta
     3901 tatgattgat tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta
     3961 aaaaaataac catattcgtt accatttttt cctccttttg ctggtctccc taccagcact
     4021 cgatttaacc ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt
     4081 tattctgctg ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact
     4141 aacgccaact tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca
     4201 tgtaagctta aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga
     4261 ataccttctt cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc
     4321 atttcttcta atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg
     4381 atttcttgta cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct
     4441 tttcttttta attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc
     4501 agaaaatagt tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct
     4561 ttcccaagac ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa
     4621 cgaacttgga tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa
     4681 ttcgtcacga ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc
     4741 aaattatttc gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct
     4801 gtatcgaaaa tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc
     4861 atcgcttctt tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa
     4921 aattgggaac ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca
     4981 ccagcaccat ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt
     5041 gttccaatcc cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct
     5101 gctttttcta cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa
     5161 gcaatatctt ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa
     5221 tagggaagat ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga
     5281 gtggacataa acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt
     5341 tctttcagac tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga
     5401 ccaatcccaa tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag
     5461 tttgctagtc cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct
     5521 tcaactggta atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc
     5581 atataaaagt cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg
     5641 cttcttcttc tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa
     5701 cttcagggta agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag
     5761 tcaatacgat aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg
     5821 tacgcaaacg ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta
     5881 ttttgttttc attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg
     5941 cttgagtcac atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa
     6001 taggttgttc tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag
     6061 acacctctca atgttgatta tagcaaaaga t
//
LOCUS       DAHUWM010000156         6047 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHUWM010000156 DAHUWM010000000
VERSION     DAHUWM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.159"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..408)
                     /locus_tag="NK788_002171"
     CDS             complement(<1..408)
                     /locus_tag="NK788_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770488.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFL"
     gene            complement(508..1017)
                     /locus_tag="NK788_002172"
     CDS             complement(508..1017)
                     /locus_tag="NK788_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCH6770489.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1218..2087)
                     /locus_tag="NK788_002173"
     CDS             complement(1218..2087)
                     /locus_tag="NK788_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6770490.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2107..3228)
                     /locus_tag="NK788_002174"
     CDS             complement(2107..3228)
                     /locus_tag="NK788_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCH6770491.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3230..3625)
                     /locus_tag="NK788_002175"
     CDS             complement(3230..3625)
                     /locus_tag="NK788_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCH6770492.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3644..4450)
                     /locus_tag="NK788_002176"
     CDS             complement(3644..4450)
                     /locus_tag="NK788_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6770493.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4443..5231)
                     /locus_tag="NK788_002177"
     CDS             complement(4443..5231)
                     /locus_tag="NK788_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770494.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5245..5721)
                     /locus_tag="NK788_002178"
     CDS             complement(5245..5721)
                     /locus_tag="NK788_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770495.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5793..>6047)
                     /locus_tag="NK788_002179"
     CDS             complement(5793..>6047)
                     /locus_tag="NK788_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6770496.1"
                     /translation="LQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETS
                     EEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1868 a         1315 c          862 g         2002 t
ORIGIN      
        1 taaaaaaata tcattagaag aagatttccc cacagtaatg accattccat cagctctatg
       61 tgttacatac aaataattgt atgacctcat ttcacctaat gaatcaatta tttcttgccc
      121 tttagctcta atatattgat ctctattacc tatctcactc ttatttaaca aaagtttttt
      181 catgtcaaat ttaatcggct gttttatttt tgtaaattcg cttctactac taatccaatc
      241 cgagattaat ttttcaacat gtcccagagt aaactctgat ttattcgcta aatacttttt
      301 ttgttgtatt aaagacatct ttccccgaag ctcaaaattg agcctacatc gattctctat
      361 ctcttcaaaa agcatcttat cttcatcatt cttatattca tcattcataa aaccaatcca
      421 gctcctcgat taagataata ttttaatctc atacaaaaac tagctctcat caaaacaacc
      481 attgttttga tttctcatga aatttcttta tacttttctc cgattataaa ttcctaaccc
      541 cttgagaaaa ttacgcatat atttatctcc gcaaggctta aaatttcgtt ggccttcttt
      601 tctcaaaaat gccccgattt cgccttttga tggatagatt tctgcctcag caaaaacgtc
      661 taaaatatca tcagtggtca tagacaaggc gatttttaat ttttttagca acacattgtt
      721 gatatatttg atttccttgc ttttttgcgg cgtgtctact atcggcttat ctttttgcgg
      781 accacgcgcc aagagaatca ggccatttag gaatgcgtca aaaacttggt tgttgatggt
      841 tttttcatac acattatttt ctgcattatc tttgttctta tcctgtactt tggtcagtac
      901 cgcctgagct tcttctttag tcagtggtac accgcctagc tcaaaagctt taatcaaatc
      961 cgtatctttc aaatcaatcg cataacgcaa tcgcaataac ctatcattgt tattcatttg
     1021 ttcctccgaa gtatttagtc tttactttct tacgtacttt ttgtcttgct aagattttta
     1081 ggatcaatga taaaaaccgc atcacttatg ataaggcgct gggtatcatg agtgaatgat
     1141 attgctgaat acaaatagtc cgaccacatt aagttgatcg gacttcataa gcattttatt
     1201 gactaatact tttttgctta agaattgatt ctaacatctt ttgctaaagt taagcatcct
     1261 atagttcctg cattatctgc taacttaggt gtgacaatgt attcttcgat atttggcgta
     1321 tcgacatagc cgttaattag ttctaaaaaa cgtctttgta ctttcttttt cagatgttct
     1381 tgttgcatga caccaccacc aaaaattata atatctggtg aaagcatcag ggtcgtatta
     1441 caggcacatt gtgcaatata atccgcctcg atttcccaga acggatcttc tatagaaaga
     1501 tcttgtccct tggttcccgt acgcatctcg atagcgggtc cagatgccaa cccttcgaga
     1561 caatcatgat gaaaaggaca agtaccagag aaagtatctc ctgaatgtct cttaactaac
     1621 gtatgcccca tttctggatg gctaaaccct tcaacaaaaa tcccatcttg tatagctcct
     1681 ccaccaattc cagttccaac agtgtaataa acaacactgg ataaaccttt tccatgacct
     1741 gaaacatact ccccgtaaca agcagcattc acatcggttg tccaggcaat tggaatttcg
     1801 aaatgcttat tcaacgttcc aacaaaatca aaatttttcc aggcagtttt tggagtagag
     1861 gtgatatgtc cataggtctt tgattcacga tggatatcaa tcggtccaaa tgaccctact
     1921 ccaatgcttt ctaattgttc tttatagtga ttaaagaaat caattaccaa actcattgtt
     1981 tcattcggtg tagttgtagg aaaactcact ctttctaaaa cctttaaatc atcattgcca
     2041 atggcacaaa caaatttagt cccacctgct tctattgagc catacatttc ttactccacc
     2101 gtccttttat ctaggaattc gttcttccct gtctaatagt ttttcgaaaa actcatgtgg
     2161 ttccttttgg taccaatagg caacagaaga aatatcgtct tgacgctcat aaagcttgat
     2221 gtcatcattt cctagggttt ggaaagtaac acgtaaattt tctttgaaac taatggggtc
     2281 gggtaaatgc cagcggtaca acccatgcat cggtaaagca tcatcgccaa aaccatgaac
     2341 tggatttgga ttaggttttc cagcactaaa ataatcccgg gtgtgatctc ttttagattg
     2401 aaaaggatag cccataaata gcgtattaaa attttgagca tatgcccttt ctccaaagtt
     2461 ctttttatgg aaagcccaag cgccgccaaa ataatcttct gctcctgtag atgttattgt
     2521 agggaaatct tcatctccat ctatataaaa cttaaattct ccttcccccc accagtatct
     2581 ttccaatgcg cacaaagcaa agtaggttcc tacataatac ccagtgcctt tgattccatc
     2641 aataagggtg taatcttttc cttttgtcgt gatgggttca cggttccatt tagcatgaaa
     2701 atacatcgca tcatccggta cttcagtcaa tgcataatta aatgtataaa aaatgtgctt
     2761 aataaactca ggatgttcat tcgttaaagt aatcttagct ttttttcgaa atggcatctc
     2821 aaaatagcaa ttcatccctg atgttggatt cactacgatt ggtaaggaat tcacttcgca
     2881 tcttgtccca aacccattac aaaaaaaatc tcctaatgga ctttctacag aaggagtgga
     2941 ttcatcatcc caataaatac gcaaaattaa atcacgtaag acgaaactcc cagcgattgt
     3001 tttgtctgtc actgtcatcc aaaaatgacg taaaattcca gggccttcca tttctgctaa
     3061 agtaattgtc tctccagcat ttatcggaac agaaggtgac ccttttctcc caattcctag
     3121 atgactcgct gtcattgccg ctttcccttt ttcaccattt ttattttctg gtgaaatagt
     3181 tctactttcc tctcccttgt ggagccatat attatttaaa caattcattt tattcgtcct
     3241 ctatcctttc ttcacttttt tgattattca tatagatgat ttgttgttgt gcctccgcaa
     3301 taattgctcc aatatctaaa ttatcatttc ctaactgcaa agcgattgac attgccaagg
     3361 gaagattcat tcctgcaatt acgtgcactc tgtcagaaat ataggtaaaa aacttttgat
     3421 ttacacttcc tccagccatg tcagttaaaa caattatttc gtctgtagta tccactaact
     3481 ctttaatttg ttcatctata tcatcaattt cttcgacata tgcacataag ctactaattt
     3541 gattatgcat acctgttaaa aactctagtg tctctttaaa accctcagct agtattgaat
     3601 ggctagcaat gatcaatctt ctcataattc aaatcctttt cctttacgct aatattccaa
     3661 atgcagccat aaccatcgca aaaacaataa cgagtagaat caaaatggtc atactgattt
     3721 ttttcttact gagtagataa tatgctgcag cagtaataat tactggaatt aatgttggta
     3781 aaattttgtc caacattggt tggattttca tactaacttc accaaaagtg aatttaagtg
     3841 gcgttgaaac actgataact gaagcaatta atgatccaac tactgttagc cccaatactg
     3901 aagcagcttc agttaaataa ttaatctttt caccgtattc agtaacaatc ttcattccct
     3961 gcttataacc aaattcaaag cctctcattc ggaaaaagaa gattaccatg attaaaatga
     4021 accataaaaa gattcctaca ggattatttt ctaaagccat ataagccgca attgatccca
     4081 tgatggttgg gatcatcgtc atcaaaagag agtccccaat accagcaatt ggacccatcg
     4141 taccaacttt aaagtcatta atcgcatctt ttgcttctaa tccttgcttt tcctccattg
     4201 caacacaagc acctaaaata atggctgcca gccaaggctg tgtattgtaa tactgaaagt
     4261 gattatttaa agcttcgatg tattcatcat catctttgta taattttctt aaaataggtg
     4321 acaatgcgta agtcacagat gcaccttgct gtgtttgata attaaaggta caaactgaca
     4381 tcaaccaacg atagttcgct ttcctaagat cctttttcgt tacttgatat ttttcgctca
     4441 ttttattcat cctccccaat aaacccaaca tcagaagctc ccgcagaagc agtgatttgt
     4501 gcatcttttt cagctctatg atacacatta aatgctaaag cgaatcctat gatcgccgct
     4561 ccaataatag gtaccttcaa aaaagcggct aagaaaaatc caacaagtaa ataactaatg
     4621 tatcgacctg taggcataaa gcgtaacaac atcgcaatac caacagcagg caacattttt
     4681 ccggctaatg ttagcccatc cataaaccaa ttaggtaaaa catccaaaac gtaattaaca
     4741 gcagactgtc ctaaagtaac ggcaataaat actggaattg ctgcagataa accaaagaaa
     4801 attggcccca accacaaaac actattcatt ttcttaaatt ctcgtttatt cgcatattcc
     4861 tgtgatttgc gaacaacaaa accatttaag attttgacaa ttacatctag ttgcacccct
     4921 aacataccta caggtagtcc tattgctaat ccggcttcta ctccaagctg gctaccaaat
     4981 gccgttgcaa taattgcgca cgttccatag tccggtacag atgagccacc aaaaccggca
     5041 actcctaatg acatcaattg aactgtaccg ccaactatca aacctgtttt tatatcaccc
     5101 atcaaaattc ctgtgaataa tccccaaaaa actgcgtaat ttacaaagat catcggaatc
     5161 ccataataat caacatgttt aataaatgct aatatagtca gcataataat ttgaaaaata
     5221 gtaaaatgca ttatccttac tcctttatcc taattttgat tgaatttcct gtgccttatc
     5281 cgccggaaca aattgtgtaa caatttctac accagaatcc aacaattttt tatacgtgct
     5341 gatattttca tctgatgcat aggctctaga agatagctgt aatgcattat ctttaacaac
     5401 agtaccgcca acaattagtc ggtcaattct aataccgtta tctactaaat gaaggattgt
     5461 ttccggctct ttcacgatga caaatacttt ttcctgatcg tatttcccct ttttaaaatt
     5521 cgcaatcgct gtttcttcag tgataattga tacagccatt ccagcaggtc tagctaaacg
     5581 catacttgat ttttggatgt cgttatttgc gacagaatca tcgatcacca tcactctgtt
     5641 tgctttagac attggtgccc attgcgtgac aataatccca tgcaataaac ggttatcaac
     5701 tctcgacata acaatactca taaatacttc ctcctttatc tttcattttc ttacaagcaa
     5761 ttaccatgcc aaaacaaaaa gaacttgata aatcaagctc tttttacata atcataaacg
     5821 tatttaacct catgaatcgg gatgtcgaca ctataataat gttcaacgac actgaaagat
     5881 tgcttaaaat gtgtctgaaa tttttgaaat tcctcactcg tttcattgaa ataaatttca
     5941 tcttccaaag tattttgttt tactaatcgt tcaattaagc aactaatgtg aacatataat
     6001 ccgaaacacg tattattatc aaatgaaatt cctagatcat tttgtaa
//
LOCUS       DAHUWM010000157         6047 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHUWM010000157 DAHUWM010000000
VERSION     DAHUWM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.160"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(259..744)
                     /locus_tag="NK788_002180"
     CDS             complement(259..744)
                     /locus_tag="NK788_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770497.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(883..1098)
                     /locus_tag="NK788_002181"
     CDS             complement(883..1098)
                     /locus_tag="NK788_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCH6770498.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1322..2122
                     /locus_tag="NK788_002182"
     CDS             1322..2122
                     /locus_tag="NK788_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6770499.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2141..4009
                     /locus_tag="NK788_002183"
     CDS             2141..4009
                     /locus_tag="NK788_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCH6770500.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4002..4493
                     /locus_tag="NK788_002184"
     CDS             4002..4493
                     /locus_tag="NK788_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCH6770501.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4481..5035
                     /gene="srlA"
                     /locus_tag="NK788_002185"
     CDS             4481..5035
                     /gene="srlA"
                     /locus_tag="NK788_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCH6770502.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5053..>6047
                     /locus_tag="NK788_002186"
     CDS             5053..>6047
                     /locus_tag="NK788_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCH6770503.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2127 a          965 c         1213 g         1742 t
ORIGIN      
        1 ttctatccaa atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac
       61 tcagttgacc gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg
      121 agtatattca taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta
      181 tactccgtat aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat
      241 ccgtataata tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc
      301 ttcgggtgac actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt
      361 gctggaagct gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc
      421 gtcactttgt aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc
      481 taatggaaga gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga
      541 ctgaatactt gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga
      601 agtagtagaa gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc
      661 tttaacagaa ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa
      721 gcctgcgtaa atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt
      781 atatcactta aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg
      841 aaggttcatg aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac
      901 ttttctagta atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat
      961 ataaatgatc ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag
     1021 ttcgaacaag cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat
     1081 agcatctaaa gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa
     1141 aaagagaaac attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa
     1201 aatttttttt gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct
     1261 taattttttt tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca
     1321 tatgtcggat tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg
     1381 aattggaaga agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga
     1441 tgtaactgaa tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc
     1501 taaaacggac ataaaagaag ggatatataa agtaatgaaa cattttaaga cgatcgatgg
     1561 tctagtcaat aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa
     1621 aagcaagtat gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag
     1681 tgtatatttg atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat
     1741 cgtgaatctt tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc
     1801 tacaaaagca gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa
     1861 tgttcgtgtg gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga
     1921 atatgaagaa gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta
     1981 cagtcgaaca agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct
     2041 agtctgctat tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc
     2101 aggaggtaaa acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg
     2161 gtaccaatta ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa
     2221 agaaatcaaa accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat
     2281 tgccggtatt gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt
     2341 tgattttttt gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc
     2401 atccaaaaga atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat
     2461 ggatgatttg tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac
     2521 attaaaagag atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact
     2581 aagaataata ggagatgagc tagaattacg actactttta ttttattttg tttctcccta
     2641 cttttcagag acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca
     2701 gaagaagacg aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc
     2761 tttgaataga atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg
     2821 ttttgaatat atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact
     2881 aactttgagc caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag
     2941 tataacgatg ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat
     3001 gcgtattcga cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa
     3061 acagcacctc tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt
     3121 ttatggtgag atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca
     3181 agcattagaa gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt
     3241 gtatttggag ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat
     3301 tgttcatacg acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt
     3361 ggggagggat atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa
     3421 caaatatttc gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt
     3481 gatccatttg actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa
     3541 aaaaaagcga ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa
     3601 aaagacgtat caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt
     3661 ggatgaagaa tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa
     3721 caaagaattt gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt
     3781 agggctaata actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt
     3841 tgccatacct gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat
     3901 cctatttgaa ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga
     3961 atcattttta aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat
     4021 tcatagtttt cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa
     4081 agcatttcaa tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac
     4141 gacctgggaa aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag
     4201 ttttagaagt cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg
     4261 tctatgcggg tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag
     4321 atcgtttgac acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag
     4381 gaaatgaaaa aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga
     4441 tatggaaaag tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt
     4501 tgctgaggga tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac
     4561 aggaatcgta ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt
     4621 aggtgaagac cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta
     4681 ccttgtgtta ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc
     4741 tcgtttcctt ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca
     4801 tacgagcaat ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat
     4861 tgctcaaggg atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct
     4921 tgtgggactg ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt
     4981 atgcagacag caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa
     5041 ggagagtttt aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt
     5101 cctttgatta ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt
     5161 gagaggccaa tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg
     5221 ttcaaaacat caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact
     5281 cttagatgtg gaatttatcc taaaaaaggg ataccaacca tcaatatcgt tcccacagga
     5341 aaaagtggcc cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta
     5401 gaacaaatac agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct
     5461 ccagataaaa agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag
     5521 caaaactgga tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa
     5581 tcagcaagag aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt
     5641 tcccttttga ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg
     5701 gcacctttag caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca
     5761 tttttatctc ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc
     5821 ggtgtagaga ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct
     5881 atcaatactc aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa
     5941 gctgagacag tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc
     6001 ccacgagtta tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAHUWM010000158         6039 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHUWM010000158 DAHUWM010000000
VERSION     DAHUWM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.161"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            16..246
                     /locus_tag="NK788_002187"
     CDS             16..246
                     /locus_tag="NK788_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCH6770504.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            343..1644
                     /gene="murA"
                     /locus_tag="NK788_002188"
     CDS             343..1644
                     /gene="murA"
                     /locus_tag="NK788_002188"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCH6770505.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1657..1833
                     /locus_tag="NK788_002189"
     CDS             1657..1833
                     /locus_tag="NK788_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCH6770506.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1968..2264
                     /gene="yidD"
                     /locus_tag="NK788_002190"
     CDS             1968..2264
                     /gene="yidD"
                     /locus_tag="NK788_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCH6770507.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2448..2915
                     /locus_tag="NK788_002191"
     CDS             2448..2915
                     /locus_tag="NK788_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770508.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3000..3884
                     /locus_tag="NK788_002192"
     CDS             3000..3884
                     /locus_tag="NK788_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6770509.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3998..4234
                     /locus_tag="NK788_002193"
     CDS             3998..4234
                     /locus_tag="NK788_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCH6770510.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4322..4402
                     /locus_tag="NK788_002194"
     tRNA            4322..4402
                     /locus_tag="NK788_002194"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4356..4358,aa:Tyr,seq:gta)
     gene            4409..4480
                     /locus_tag="NK788_002195"
     tRNA            4409..4480
                     /locus_tag="NK788_002195"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4441..4443,aa:Gln,seq:ttg)
     gene            complement(4634..5935)
                     /locus_tag="NK788_002196"
     CDS             complement(4634..5935)
                     /locus_tag="NK788_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6770511.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1963 a          987 c         1312 g         1777 t
ORIGIN      
        1 agaaaggacg tcattatgca agtatacgga attgatgcgg tgcttcgaat cgtgagtcat
       61 ttggcgttta tctacttagc cttctggtct ttacgttcgt tacgcattga aacttttttc
      121 agaccaatgc ataatacgca aattagaatg gcaatcgtac ttttttcaat ttttttaggg
      181 tacactgcaa gtaatttttt cttagaagtt atcgctttat gccgaaatct tttcgttagc
      241 ctgtaaattt taaaaaaagg tactatttta aaagcaagga tgttataata gaaaagattt
      301 tgagtaagtg tatgctcatt ggaatttttt ggagggaaca acatggaaga gatcatcgta
      361 agaggtggca atcaattaaa tggaacagta agaattgaag gagctaaaaa tgctgttttg
      421 cctattttag ctgctagttt gttagcagaa gaaggtatta cgacattaga caacgttccg
      481 attctttctg atgtcttcac tatgaatcaa gtgattcgtc acttaaacgt agatgtagat
      541 tttgatgaac aaaagaatca agtaacaatc gatgcttcaa gacaattaga aatcgaagcg
      601 ccttatgagt atgttagtca aatgcgtgct tcaatcgttg tgatgggtcc gctacttgcc
      661 agaaatggac atgcaaaagt agctatgcct ggtggatgtg cgattggtaa acgaccaatc
      721 gatttgcatt taaaaggatt tcaagcttta ggagcaaaaa ttattcaaaa aaacggctat
      781 atcgaagcaa ttgcggatga attgatcggt aacacgattt atttagattt ccctagtgta
      841 ggtgctactc aaaatatcat gatggctgcc gtaaaagcaa aaggaacaac catcattgaa
      901 aacgtagctc gcgaaccaga aatcgttgat ttagcaaaca ttttgaataa aatgggcgct
      961 caagtctatg gagctggtac tgaaacaatg cgtattgaag gcgtggatca tttacatgca
     1021 gtcaaccact caatcgtaca agatcgtatc gaagcaggta cctttatggt tgctgctgca
     1081 atgacacaag gcaatgtgct gattgcagat gctatctctg aacataaccg cccattgatt
     1141 tctaaattga tcgaaatggg tgcagaaatc attgaagaag aaggcggagt ccgtgtcatc
     1201 ggaccaaaac atatcttacc tacagatgta aaaacgatgc ctcatcctgg tttccctaca
     1261 gatatgcaag ctcaaatgac agctatccaa ttagttgcag aaggtacaag tgtagtgact
     1321 gaaacagtgt ttgaaaatcg tttccaacat ttagaagaga tgcgccgtat gaacgctcat
     1381 gtcaaaatcg acggaaatgt agcaatcatg gatggaaacc atgaattgca aggtgcagaa
     1441 gtatatgcaa cagacttgcg ggcagcagca gcattagttt tagctggctt gaaagcaaat
     1501 ggaatcacac gtgtgcgtaa cttgaattat ctagaccgtg gatattataa tttccatatc
     1561 aaactacagc aattaggtgc agacgttgaa cgtgtagaca tggatcaaac ttctgctgaa
     1621 aaaacagcac aaacgattgc ttaaggaagt ggctgaatga gtaaccaacg atatatctta
     1681 ttaactttga taaagatttt agtggtgatc ttattgctta tccttctttt tgtggccgga
     1741 acgatgatcg gatatggtgt catcggtggc ggcaatccat ttaaagtatt ccagccaagt
     1801 ttgtggattc atattcgaga cttttttcat tgataaataa gaatcacagt ggttctagtc
     1861 gatatgaagt gagccatgaa gcagaacatg acaaaagaaa acagaaggga acgttgacgt
     1921 tagtcgcgat cccttttttt agtaaaagaa aagaaggaat ctaattgatg aagaagatat
     1981 tgatttattt agttagagga tatcagcgct ttatttcgcc tgcatttcca cctagctgcc
     2041 gttattaccc aacctgttcc aattatatga taaaagcaat tcaaacccat ggtgcactaa
     2101 aagggacggt aatggggact gcacggatct tacgctgtca tccttttgtc aaaggcggta
     2161 tcgattatgt accattgaca ttccaattaa cgcgaaataa gaaagataaa aaagaaccag
     2221 attataaaaa ggttgtgaaa aatacgagta gcactgagcg ataaagaaac aatttggaaa
     2281 atagcttctt atattttgca aaattgtgag ttattgccga agtgttggat tttgaacacc
     2341 gtcaaaaaaa ggttgtgaaa aatacgggta gcactgagcg ataaagaaaa agaggaaaat
     2401 tattgtcaaa gtaacaactc cgcaactact tatatggggg acaaaaaatg atcgaagaac
     2461 taaacaagaa agatttacca tggtccttgt tattagatgc agatcctgat aaagaaaaag
     2521 ttcaagtata tgtttccaga gggagtggct ggatatggaa agaaaaagaa aaaacaatag
     2581 gtgtactgat ctatgtggca agggaaaaag aatacgaaat cgtcaatgta gcagtagccc
     2641 caacgtatca aggaaagggg attggcggaa aattacttga gacagctttt cagaagcttt
     2701 cacaactcgc ttccagtcaa actcagatca tcatacgaac cggcagtact agcagtgcgg
     2761 cgttacatct ctatcaaaaa atgggctatg tggagattgg tcgagtcaaa gactatttta
     2821 tccatcatta ctctgagccc atttttgaaa atggagatca attaagggat caagtaattt
     2881 tagcaattca cttacctaaa aatagcttta actaataaac tttcaattcg agaaaatgct
     2941 ttcaaatgct aaatagatac attacaatag ctttagcaaa caaaaaggag aatattttta
     3001 tgggaatatt agtttttgat tttggtggat cggctgtcaa atatggttgt tgggatggca
     3061 aagagattaa aggtaaggga caatttgcaa cgcctgaatc ttgggaagaa atgaaagcgc
     3121 aattatttca agtttataaa aaaatactcg tttcttttga agtcgaagga attggaatca
     3181 gctctcctgg agtagtgaat aatgagaaac aggtgattga gggaatcagt gcgattcctt
     3241 atattcatgg atttaatatt ttccgagatt tggaaactct gtttcaactc ccagtaacga
     3301 ttgaaaatga cgccaactgt gccggaatgg ctgaatttta tgaaggtgct gcccgtgatt
     3361 atcaagatgt tgcttttgtt gtggcaggaa ctgggattgg cggggcgttg ttcacgaaag
     3421 gacaaatcaa taaaggggca catctttatg gtggagaatt tgggttgatg ttcttaaatg
     3481 gcgataagac attcagtaaa cttggtacag cggtacagat ggcttggcgt tattgtgaac
     3541 gcaaaggtct cgataaaaat tcttataccg gaaaagatgt ctttgaatta gcagaaactg
     3601 gagatgcaat agcaaaagaa gaagttgaga gtttttatac gtatcttaca aagggattat
     3661 tcagtattca attttcgttt gatccagaag tgatcgtatt aggcggcggt gtttctgcaa
     3721 aagaagggct cattgatgag atcaaaagcc gaatgaagaa gctgacagaa caattcgatc
     3781 tgcatgattt cgaaccgcag attttattat gtgaatacag aaacgatgct aatcttgtag
     3841 gtgcagcagc aaactatctc gctgtacgtc aaggggagaa ttaagagctt tagagaaggt
     3901 gcagcttgca taaaagaagt gaatgaaatg aataggagat ttttacctaa ggaatgtttt
     3961 atttgctgca gtatgataaa atatctgaga ggtgaaaatg atgtcaaaaa aaaataaaga
     4021 tgttgaagtg cgtatagaag aagtgaaaaa agatatcaaa ggtaagaatt atgaagtcac
     4081 tcagttattt atagggaaaa aaatgattgg tgagatctta gcctatgggc caaaagaata
     4141 tgaaattttc tttggtgaag aagattttgg aaaagaaaaa agtcttgaaa atgcgataga
     4201 aacagtgatt cgacactgga atctgcacga ataaaaaaat atcaaaaaaa ctttcaataa
     4261 agggttgcaa aaatctggaa aattaggtat tataactaag tcaggttgat acaaaccttt
     4321 cggaggagta gcgaagtggc taaacgcgac ggactgtaaa tccgttcctt agggttcagt
     4381 ggttcgaatc cactctcctc catttcattg gggtatagcc aagcggtaag gcaagggact
     4441 ttgactccct catgcgttgg ttcgaatcca gctaccccag tataccacaa tcgtaagctc
     4501 tcgttagttc gagagctctt ttttttgctt tgctaccttg ttccttgaag attctgctga
     4561 tttaagaggc tctttgtcaa ataggactga tagagtgaga taagaacatc taaggtagaa
     4621 ttccttttct actttagatg ttttttgatt cataggtaat cagattattt agtaagaaaa
     4681 ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc aatgctttga
     4741 tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg gctaaacgaa
     4801 tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt ggaacggaat
     4861 tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga aacgcttgaa
     4921 gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa agcagacgat
     4981 ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg gtccatttgt
     5041 cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt aattgtcgat
     5101 aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt acgcgaatat
     5161 cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg agttttgcct
     5221 ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc acgatgacct
     5281 ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga gcctttttct
     5341 tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa gaaaaggagt
     5401 agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa cgcggtaacc
     5461 aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa acggtcgacc
     5521 aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt agctcttcta
     5581 aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg taggtattcg
     5641 ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa gcaagataag
     5701 tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg ttcttaacga
     5761 tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct ttacttaacg
     5821 tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca tttgtgatga
     5881 aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca ggcatacaga
     5941 acgctccttt gattgtgttg tggtgattca attctaacgg agcttctgta tgttttgttt
     6001 tttcatgctc aaaagggacc gatagaatga ttttctatc
//
LOCUS       DAHUWM010000159         5914 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHUWM010000159 DAHUWM010000000
VERSION     DAHUWM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.163"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            24..632
                     /locus_tag="NK788_002197"
     CDS             24..632
                     /locus_tag="NK788_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6770512.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            800..2437
                     /locus_tag="NK788_002198"
     CDS             800..2437
                     /locus_tag="NK788_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCH6770513.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2509..3621)
                     /locus_tag="NK788_002199"
     CDS             complement(2509..3621)
                     /locus_tag="NK788_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCH6770514.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(3696..5069)
                     /locus_tag="NK788_002200"
     CDS             complement(3696..5069)
                     /locus_tag="NK788_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6770515.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5290..5907
                     /locus_tag="NK788_002201"
     CDS             5290..5907
                     /locus_tag="NK788_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCH6770516.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
BASE COUNT         1983 a         1184 c         1191 g         1556 t
ORIGIN      
        1 acggagaaaa aaaggagttt aatatgaatt catttaagaa aattgtttta ggtacaactt
       61 ttgctgctgg tgctactgca atgtttgtag gaactacaaa tgctcacgct gatgaagttt
      121 atacagtaaa atcaggtgat tcattatcta agatctcaca aaaatttgca ggagacaact
      181 caatgatcga tgcaattgct gaaaaaaatt cgatcgcaaa catcaaccgc atttatgttg
      241 gtgaacaatt aacaatccca acttcaaatg attcttcagc aacaacagaa aacaaaacaa
      301 cagaaaatac agcatctaca actgaaacag caacgcaaga acatacatac gttgcacctg
      361 ttgaaacagt tgaagtagct cctgctgccc cagctgctgc aacagcgcca acttcaagct
      421 ctgcaaaaga atggatcgca caaaaagaat caggcggttc atacacagct actaacggac
      481 gttacatcgg ccgttatcaa ttagatgctt catacttaaa cggcgactat tcagctgcca
      541 atcaagaacg tgtagcagaa caatatgtaa catctcgtta tggttcatgg gatgctgcta
      601 aaacattctg gttagcaaac ggctggtact aagccgataa aaggaaaatg atgaaaccga
      661 gaaacttgtt tattcaagtt ctcggttttt ttgtgtgaaa aataactcgt ttgcttccca
      721 cagcgcagca gtacaaataa aaactcgaat aatgtataat aaagaaaacg tttttttgtt
      781 tagggaatga ggaggagcca tgagtacaaa agagagtctc gaattaaacg aaatcacaag
      841 tggaaatcca ctgttatcca gtattcgatc gattgtagaa acgacatttt acggtaataa
      901 tgttcatgaa gtttttgaca ggaagacagc ttatcaacta gcaaaaggga gtccaggaac
      961 gatcatcaca gatttaacaa tctcacatgc tgaagaacta gatttaccag ctgatgttag
     1021 gacactagta ttcaatgatg gaagcattgt gggaagaaca gcaagtgctc ggcggatctt
     1081 tgaggactta gacaaggaac aaagcaaata cgagaaaatt ttaagagaag ctgtgtatca
     1141 aagtcgcaag cgccagtttt accatacaaa agtaatcgtt ggcctatctg aagaattttc
     1201 tgtgcaaagt catttgctga ttccagaagg atttgagaac aatttgtatt cttatttgct
     1261 gaattttcag ccagcgaatt cgaagatctt agagacttat caaaagtctg tttcctttga
     1321 tgagggagat ctttatatct tttgtgatcc cgaatggaca catgaaacat ttccaaatgg
     1381 actcattttg tttgatgctc tccataatac agcggctgtg ctaggattac gttactttgg
     1441 agaattaaaa aaagcgactt tgacgcttgc ttgggcattg ggaaatcgca atggctttat
     1501 tgcctgccac ggtgggatga aacaatatgc tagtaatgac cagacctata cgatggctgt
     1561 atttgggttg tccggttctg gaaaatcaac gattacctta gcaaaacata cggaaggtca
     1621 ctctgtttcg atcttgcatg acgatgcttt cgttatttct cgaaaaaatg gttttgctac
     1681 tgccttggaa ccttcttact ttgataaaac acaagactat ccgtctgatg atccggccat
     1741 ttcttctttc cttacttgtc agaatgtggg cgtgacatgt acaagtaaag gagaaaaagt
     1801 cctagtaaca ggagatatac gaaacggaaa tgggcggaca gtcaaatcac gttatgcgac
     1861 acccgaccga gtcgatcatc ttagcgaaaa gatcgatgct gttttttgga tcatgaaaga
     1921 tgattcgctt ccgccaatta tcaagatcaa tgacccagtt ttagcagcgt cttttggtgc
     1981 aacattagca acaaaacgat cagacgcgga aaatatcact acaaaaagac agctagatga
     2041 gctagtcatt gaaccatttg ctaatccttt ccgtgtctat cctcttaggg aagactatca
     2101 agcttttcga gagttgtttt tgaaacagca aacagcctgc tatgtcctga atactggata
     2161 tttcgatgat aaaaaaatca aacctgaaac gacattgtca atcgttgaaa gaatcgtgca
     2221 acaaacggca gaattcaagt cgtttggtcc atttgaagac atccactatt tacctatacc
     2281 agattttcct gtttcttttg aggatacgtc ctataaaaat ctggtgaaac aacgacttga
     2341 tattcgttta cgattcattg aagctcagat aggaaaagat ctcttgccag atgaagcaaa
     2401 agaaagtttg gagaatttga ttcagaaatt gaactaaaat aacagctttt tcaagaagta
     2461 atccatgcgc aatatcgctc gggatcctta cttgaaaaag ctgtttttct atgatggatt
     2521 tttcgtaata taggtggaga tgtccttttg ataatcttcc gtagaccaat gaccagtatt
     2581 taagaagttg gctaatagat agccgatata aggacctgtg gtcaagccag aagagcctag
     2641 accgctagca gccagtaagc ttggatcatc tggcagtacg ccgaaaaatg gggaaaaatc
     2701 tgacgtataa gcacgggtac ctacacggaa ctcgtatggc cagtttttta tctgatctgg
     2761 atcagtcaag aactgttctg tactcttagt caatccctta taagcttcgc tagtaggagc
     2821 caaatcccag ccttgatcgt tttcatgggt agcacctaaa aggatctttc catccatgaa
     2881 aggaatgaaa tcagcttctc cgtcaagcat agcaacaggc cagtcttgac tcatcttata
     2941 agatgtctga aatgctaaga gctgcccttt ttgcggacgt atatctgcag catagcctaa
     3001 ttcttgtaat aaaggcttaa gtgccgggcc aggtgtcaaa attacacgat cagctgtgta
     3061 ttctccaaac ggtcccttga tgagccaaga atcgctcttc ttatgcaaag tgacttttgc
     3121 ctggatctgc tccatttgtt tttgtgctaa acgctgacca agatgttcta aatatgcttt
     3181 tccatccaga cgaccaccac cactgatacg cagtgaaact gctggtttca ataacggaag
     3241 tgcttgagat gtctgttcag gtgaaagcca ttcgatgttt ccaatttctg gcgcttcttc
     3301 ttttcgtttc tcggcaagaa cagccaattc agctaaatct ttttcaggtc gaagcaaaag
     3361 tgtccctgat tgtgcgtaaa tcgtttgatc aagttggaaa tcttttataa gtttagggaa
     3421 aaaagcagcg ccgtctttag ccagacgata ccattgtttg tttctgcgtt tcgacaacca
     3481 aggggagata atcccagcgc tagctttagt tgcctgtcct gtcccttcat caaataaagc
     3541 aacattgaat tttgatgtat ctaagtaact ggctagagtc ataccaatga tcccgccacc
     3601 gataatggca attttttcca taaaaaagcc tccttttaca aacaaccatt ttcacacaaa
     3661 aaaacggcag atgcaacacc tgccgtatat ggaatctacg cttttttagc aaagaacaga
     3721 taataggcga atcgagcaaa gcttacgaga agcaacagca acaagacgcc taatgctgct
     3781 tttgatccta atgtagtaga cacttcatag ttatgggaag tatgatgctg gacgtaattg
     3841 ataatgaccg ctactaatga agtagccaca gcgccagaaa actgctgcaa ggtattgaac
     3901 attgcgttcc cgtctccttg tagttcttca ttcagtgcat tcattccggt ggtcatcata
     3961 ttgctgtaag ataatccgat accgatcata taaaacacat gacaagcaat caatacaccc
     4021 actgaagcgt gtcctatgag taggaataat gctagccaac caatcaaaga aagaatcagc
     4081 ccagcaaaaa ttggcttctt cggaccatat tgatccagca ttttcccgga aaaaggagaa
     4141 agcaaagcac caaccgctgc gccgggaagc atcgctaatc cagcgacaaa aggactactt
     4201 ccttgaacga tctgaaggaa attcggcaag acaaaagata caccaagtaa taaaaactga
     4261 taaactaaga agccaatcaa aaacaagcgg aatgttttat ttgaaagaat agacagtcgg
     4321 atcagtggat gctccacttt tttacttcga tagtaaaaaa ggaaccagcc aataccgccg
     4381 atgattaaag caatccagct ggaaactgtt cccaacgtac tcaaaaatgt tagacttcca
     4441 ctaaacaaaa gacaaattcc taaaacgctt atccaatcca aataaccact tttttgtact
     4501 gccatctcgg gaatagcaaa ccagccgata atcaaagaaa gaatcaaaac gggcacaaga
     4561 aacaggaaga tatgattcca tgtaaaatgt gaagtcataa tgcctccata agtggggcca
     4621 attgctggag caatggccgt tgtcaaagta ccgacaccca tcatcgcgcc tcgtcgattg
     4681 aggggggcaa atgtcaagat aatatggaac atcaacggaa gagcgatacc tgtcgctgca
     4741 ccttggagca atcgaccaaa caataggacc atgaaggtag gagagaagaa atcgatcatt
     4801 atacctgcca agaaaagcag atttgaagct aaaaagagtt ttttgagact aaaattgcga
     4861 ttcaaataag tagacagcgg aacaatgatc gaaatcatca aaagataaat cgttgttacc
     4921 cattggactt gtgcagtcgt taaatcaaac tgtctgataa gagttgggaa agttacattc
     4981 atggccgtct cgatcaatac gcccgcaaaa gacatcatcc ctgttgcaat tactgcaggt
     5041 actaaaagca gctgtttttg ttttgtcatt tacatcacct ctaaaaaatt tcgtaaaaaa
     5101 agagagtgat ttctattata aaaatagaaa ccgcctctct ctcgacacgc cgaaacgtgt
     5161 tatctgtacg atttatttac ttcacttatt ctagcacaaa ctgaaaaata gtgtcaacac
     5221 gattagccaa gtcactcaaa aagaggtatg ataataaata actaaatgga atttcgcgga
     5281 ggaagaaaaa tggaaaaaat tgaatggcgt aaagacaaga gctatagtat gaaaaaaagc
     5341 ccgcaaatac tagaggttcc taaaaaacca ttctttagca tcagagggat cggcgacccg
     5401 aatcaggaag actttaaacg aagagtagcc tgtctatatg cagtcagcta tgcaatacgc
     5461 atgtcgccta aaaaaaattg gctgattcct gattatcaga tgtatactgt ctatccactg
     5521 gaaggacaat ggggactgca agaaaagtat ttgcatgaac cagtgatgaa aaaagaacat
     5581 ttttcttatc aattgatgat ccgacagcct gactttgtta cagaagcgat agcacaagaa
     5641 gccattcagc gttctagcaa acttccggaa gacttgaaag aacaactagt attcaaaaag
     5701 atggaagaag gtctttgtgc ccaaatctta cacgtcgggc cctatgccga agaaccagca
     5761 acgtttgaga aattagaagc attcatcgca aaagaaggct atgaaagagc ttctaaagaa
     5821 cacaaagaga tttacatcag cgatccaaga aaatcagcac ctgaaaaaat gaagacgatt
     5881 ttgcgtgtta aaatagaaaa aggttagcaa cctg
//
LOCUS       DAHUWM010000160         5889 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHUWM010000160 DAHUWM010000000
VERSION     DAHUWM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.164"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..985)
                     /locus_tag="NK788_002202"
     CDS             complement(<1..985)
                     /locus_tag="NK788_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCH6770517.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="NK788_002203"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="NK788_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCH6770518.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="NK788_002204"
     CDS             1810..2964
                     /locus_tag="NK788_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCH6770519.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="NK788_002205"
     CDS             3179..4147
                     /locus_tag="NK788_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCH6770520.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="NK788_002206"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="NK788_002206"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCH6770521.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="NK788_002207"
     CDS             5177..5785
                     /locus_tag="NK788_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCH6770522.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1776 a         1148 c         1330 g         1635 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtatt
//
LOCUS       DAHUWM010000161         5887 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHUWM010000161 DAHUWM010000000
VERSION     DAHUWM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.165"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..495
                     /locus_tag="NK788_002208"
     CDS             <1..495
                     /locus_tag="NK788_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCH6770523.1"
                     /translation="SEVLPAIRKHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRL
                     IAEQKIAEYEPKISYLDSILSSTDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQ
                     WLLCKKHMNQGYTKSHTTEIPKADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDL
                     EEIG"
     gene            510..713
                     /locus_tag="NK788_002209"
     CDS             510..713
                     /locus_tag="NK788_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770524.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            729..1049
                     /locus_tag="NK788_002210"
     CDS             729..1049
                     /locus_tag="NK788_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCH6770525.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            1054..1224
                     /locus_tag="NK788_002211"
     CDS             1054..1224
                     /locus_tag="NK788_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770526.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(1231..2013)
                     /locus_tag="NK788_002212"
     CDS             complement(1231..2013)
                     /locus_tag="NK788_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770527.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            2064..2771
                     /locus_tag="NK788_002213"
     CDS             2064..2771
                     /locus_tag="NK788_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCH6770528.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            2949..3290
                     /locus_tag="NK788_002214"
     CDS             2949..3290
                     /locus_tag="NK788_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770529.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            3283..3954
                     /locus_tag="NK788_002215"
     CDS             3283..3954
                     /locus_tag="NK788_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCH6770530.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3960..4646
                     /locus_tag="NK788_002216"
     CDS             3960..4646
                     /locus_tag="NK788_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770531.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            4653..5504
                     /locus_tag="NK788_002217"
     CDS             4653..5504
                     /locus_tag="NK788_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCH6770532.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            5516..5785
                     /locus_tag="NK788_002218"
     CDS             5516..5785
                     /locus_tag="NK788_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770533.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
BASE COUNT         2215 a          914 c         1165 g         1593 t
ORIGIN      
        1 agtgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa
       61 gaagctttgc ttaatccaga tacaatcatt caattagcaa ctcaactaaa agaagaaaga
      121 actggaagat taatcgcaga acaaaagatt gcagagtacg aacctaaaat ctcctattta
      181 gatagcatat tatcttctac agattcagta acaattagtc agattgcagc agattatggg
      241 atgtctccac aacagatgaa taaattactt cataaactag gtattcagaa aaaagtcggt
      301 aaccaatggt tattgtgcaa aaaacacatg aaccaaggat acacaaaatc tcatacaact
      361 gagatcccga aagccgatgg tggcactaaa attgtaatga ataccaaatg gacacagaaa
      421 gggcgtctat ttatctacga attactaaaa aaagaaggat attaccctca aatggattta
      481 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
      541 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgctcg
      601 ctgaaagatt cggcattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
      661 ctgcagaagc aaatagaatc atagattcga ttattactat gtacgaattg taaagggagg
      721 aacagctaat gcaatatcta gaagcgaaaa tcccagttcc agaaggctat gtaattatct
      781 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
      841 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
      901 acccaagata tagaaatatt atcgatatcg aaaaaaacaa aaatggattt gtctattatc
      961 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
     1021 caaatttttc ggaaatctta aggaggtaag cggatgggta aatttaatag agcactagta
     1081 ttcagcgcac cactaattat ctacgcttta ggtctttggg gaagcagaca agcattaatc
     1141 ggcacaattg tatatatggt ctggatcttt atggggcttg atgaagctga atatagagcg
     1201 aaaaagccag tcgggaggga ctgacttaac ctaattaatt aaaaattgta acaaataacg
     1261 caaaaataaa gctaatgaaa ttgtattgtt aggatctact ccaatagtta tatttacaat
     1321 ttcggttact actagtccta tcaaagctgc cccagtttgc tctaaatacc acattttatc
     1381 tttgtagaaa gcaggtgaag cttgttgttt ttcagatggt tcattagtta tatctttgtt
     1441 cttggctgta tttatttctt caaaaagctt tatagaatca tcaaggactt tcctttgctc
     1501 attaaggata tcaaaaaact tagcctcaaa ctcatcagat ggattagaag tgtctttaaa
     1561 aagtagattt tttgtactaa cttccggttc ggaagaaaat gctttttcta attcttgggt
     1621 agctacccct attctgaagt cgtacttaga catgtcaacg gcaaattttt ctgtgatttt
     1681 agagatatca gaaaaataag gttttggcat agtagctgcc atttttttag caatattaaa
     1741 acttggagat tctaattgtt tctgtattgc gaacaaagaa ggaggaatca tattagcaat
     1801 agatttgtta cgtttctcga caatttgaat gagagaatca ttgagtattg cttgcgaagc
     1861 tataaaagta tcttgaattg caattaattt tttagcttgt tctatcgcag gctgcatagc
     1921 gttaacgaca tctagagaag gagcaatggt ttcaccgatt aattttgaag aagctatcaa
     1981 tttttgataa taaggagtat ctgaaatatc caattatata caccaccttt ttacctaaat
     2041 tataccaaaa gggagagaaa aaaatgcaag aattagtaat tttgaaaaat aaagaagctg
     2101 tgactacgag cttacaagtc gcagaaacat tccgaaagga acatagagca gttttaaagg
     2161 cgattgacga tctaaaagat gggcttgcac aaaagtatgc agacctattt tgggaagatg
     2221 tgtacatcca tccgcaaaac aaacaggctt atcgaattgt ctatatgaat cgtgatggct
     2281 ttactcttgc tgtattagga ttcaacaata ctcaaaaagt tttagagttc aaattgaaat
     2341 acattgaagc gttcaacgaa atggaaaagg caattcgcaa aaatacagct tttgacacaa
     2401 ttgaagacat gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca
     2461 tgcttaaaaa tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa
     2521 atacgaaagt tatggaagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca
     2581 aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg
     2641 gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttaccag
     2701 aaactgcgat tcgcatggaa atcgatcaac tgaacagaca acaaagactt tttggtgatg
     2761 aaaatgaata gagctgaagc gctaagaatc gggacggtaa ttgctaatcg ctggtggaga
     2821 cacaataaac caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa
     2881 ataaaaaagt gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat
     2941 tttagcatat ggaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg
     3001 gcaaatcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacggatg
     3061 aagaactagc atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct
     3121 atcttatcca tttatcgagg aagtccaaaa aacctgaata tatctatgtc atcgaagatg
     3181 gcttgattga tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg
     3241 gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaacaaaggg
     3301 acaacgactt ttgaaaaact ttttagtcgc aagttaaata aaatactcaa gaaaaaagga
     3361 aattttgatt atttatcttg ggctcacgca tgggagatta tgaaaaagaa tgatccacag
     3421 gcaacggtaa ctattaatga gtataaacac tacagagttg tttctggaac tcatcaagac
     3481 tttcttgttg aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc
     3541 tcagtaacgg ttaaaggaca cacggaaacc gaattatttc ctgttttaga ttatcgaaac
     3601 caaccagttg ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg
     3661 aaagcattgg ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca
     3721 ccacctagaa tcgatacgaa gaaattaaac atgctagaga cgattctaga agctttcaat
     3781 gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat
     3841 aaattagggc tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt
     3901 gccttgatgg agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag
     3961 tgtttaaacc attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca
     4021 taggtgcaac catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct
     4081 acgatggtga tcgagttatt gaaattcgtt ttattgatcc tagacgtttc acagtacagc
     4141 aacgaaactt catctatgca ctcataggcg atattttcat cgatacaggc atgccaacgg
     4201 acttctggaa ggaattcttt tacttccgct ttgaaggtgt cacagggcgc aaaataagcc
     4261 tcaaagatga atcgaatacg actgtgagtg atgctaacgt cttagcaaat atcatcttag
     4321 atttcatctt tgaacatcat attcctttca aagaaggcta tgagatttta ccagcgaatc
     4381 aagaatatta cttctacaaa tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag
     4441 gagctgatat cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc
     4501 attcagagta cacatttgca gcactctgca gaatccatca cacggagaaa cacaagatag
     4561 gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat caagttaaac caggaaacaa
     4621 ttaagaaact tagaatagga ggataaaaaa tattggctga caacaaacga tactactatt
     4681 taaaactaaa agagaacttt ttcgatagcg acgagatggt tcttttagaa agtatgccag
     4741 atggatacat ttattctaat attcttctca aactttattt aagaagtctg aaacatgaag
     4801 gcaaactaat gtttaatgac aggattccat tcaattctac aatgcttgcg actattacaa
     4861 gacactctgt gggagttgta gaaaaagcag tacaaatatt tcgtgattta caacttattg
     4921 aggtattaga taacggagca atttatatgt ctgatataca aagttttatt gggaaatctt
     4981 caactgaagc tgataggaag agaaaataca gaaaagaaat tgaggaagca aaacagaatt
     5041 taataacaag tggacaaatg tcggacaaat gtccggacaa aactacacca gagttagaga
     5101 tagagttaga gaaagatata gagttagaga aagatataga gaatgtaacg ccttcgaaaa
     5161 aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt cttttgtcag
     5221 atgagcagat ggagaaactc aaaatagaat tccctaatga ctaccaagag cgaatagaac
     5281 ggctatctga gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc
     5341 gaagctgggc aagaaaagaa aaaagtgaat ctaagaacgc aagcagtgga tacaagcgca
     5401 caggaagacg agagaagctt cctgagtggg caatcgacca agaagcctat ctcaagaaaa
     5461 aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg ggaaattgaa
     5521 aatcgattat ctagaactaa ttaatgaaat agcaaagtat aaaactggtg aggaaataga
     5581 aatcctgaga gacgtatatg atcaactcga agaagctgga atcgaaggaa ttaagaatga
     5641 tcgttcgagt tggagtaagc tcagatacta tttcgcactt tatatcgata caacacaatt
     5701 aagaaattta gcatatacca aattactatt tgttgattgt gttaaaggct tgcaaaaaca
     5761 tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa gacatttaca gcacagattg
     5821 aactaatgca tcaagaagct ttaagacaaa gtgtatcgta cgaagacaag tggctcaaca
     5881 cgttcca
//
LOCUS       DAHUWM010000162         5863 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHUWM010000162 DAHUWM010000000
VERSION     DAHUWM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.166"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            46..3273
                     /gene="fss3"
                     /locus_tag="NK788_002219"
     CDS             46..3273
                     /gene="fss3"
                     /locus_tag="NK788_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCH6770534.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3386..3700
                     /locus_tag="NK788_002220"
     CDS             3386..3700
                     /locus_tag="NK788_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6770535.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3713..4087
                     /locus_tag="NK788_002221"
     CDS             3713..4087
                     /locus_tag="NK788_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6770536.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4097..4432
                     /locus_tag="NK788_002222"
     CDS             4097..4432
                     /locus_tag="NK788_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCH6770537.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4515..5855
                     /locus_tag="NK788_002223"
     CDS             4515..5855
                     /locus_tag="NK788_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCH6770538.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
BASE COUNT         1959 a         1001 c         1318 g         1585 t
ORIGIN      
        1 cgagcatctt atttttttgt gcttaatttg aaaggagaac atttcatgta taaaaaacaa
       61 aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaagcaat tgttggagca
      121 attgctccta cgattacttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa
      181 ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca
      241 gaagtcactg ttccgcatta tgcagtgtac aacggcttta aacaagatat tttctgtatt
      301 gaaccaggag tcccgattta caatgagttc actcctggct atgagaaaaa cccgttacca
      361 gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca
      421 gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac
      481 tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaataaat
      541 caagccattg acgattatca gaagaaacca accttccata atagtacggc taaaacagta
      601 ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag
      661 gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt gataatcacc
      721 ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta agaaatctgc tggtaccgga
      781 acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc gattgataaa
      841 cctaatacct acacaatcaa aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa
      901 gtcgataaag aatcgggtga tgttgtacca ggaacggttt tccatttaga ctttggaaaa
      961 actttacctg caaaagacgt gactactgac aaagaaggca ttgctacatt ggatgagatt
     1021 cctcatggta caaaagtaac cattaccgaa aaatcagtac cggcgcctta tacgattgat
     1081 accaccccca tgactaccac cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac
     1141 gcacgggaaa aagggcaaat cattctggat aaaagcggag ttgaaacagg gagtgatctt
     1201 tggaatgaca attattcctt agctggtaat acgtttgcca ttcgcaaaga cagtccaacc
     1261 ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc atgcggaaac accaaaagag
     1321 attgctaatg cgttggaatt gggaacctat tatgtgaccg aaactaaagc tagtcatggg
     1381 ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc
     1441 cctgttacca gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac cactttgacg
     1501 aaagaagaca aagatactgg ggataaagca caaggtaagg cagtctttga gggtgctgaa
     1561 tacaccttgt ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca
     1621 gagttagcga agggaacgaa aggttctgat gaaacggtca ctttgacttt agacgaaaag
     1681 aaccaagttg ccgttaaaca tttagctatc aacgagtatt attggcaaga aacaaaagcg
     1741 cccgaaggat atagccttga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat
     1801 agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa aagaacaagt gattcgcttt
     1861 ggctttgatt tctttaaatt tgctggatca gctgcgggca ctaccgaaac tggcttcaat
     1921 gatctgacct ttaaagtgtc gccattggaa gggaccaatg aaatcacagg tgccgaagat
     1981 gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat
     2041 cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt
     2101 acgccattag aaatccgttc tacttttaag gaaaacaaag aagactttgt gaagagtgag
     2161 tatgtcttta ccattaccga acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct
     2221 tacgagaagc tgacgaacaa agcattttct gttagtttga accgtttaat gctctatgat
     2281 ttgcctgagg aagaagatag tttaacttct cttgcgactt ggaaagacgg aaacaaagaa
     2341 ttaacttctc tggattctac cgagctagtt gataaattga gttacaatct acatgaaatc
     2401 aaagaagact ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa
     2461 gaaaaagatg aaaaagccaa accagtggtg attgcagaaa caagcgcaac gttggcgaac
     2521 aaagagaaaa caggaacttg gaaaattcag cataaattaa ccgccgaaca agttttaaat
     2581 aaaactattg tcttattcaa ctatgtgtat gaaaataagg aagcttttga agcaggcgat
     2641 aagccagtag cgaaggatgt tagcttgaat aaccaagccc aaactgtcag ttgtacagtg
     2701 gaacaccatg tttctatcca aacaaaggcc cacctagaaa acggttctca gacctttact
     2761 catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt actggatggc
     2821 tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac aaacaaagaa
     2881 atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac caaaactgtg
     2941 cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa
     3001 atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa
     3061 tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa acaaccggaa
     3121 acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac cttccctcaa
     3181 accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat ttttgcgacg
     3241 gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca agtaaatcct
     3301 aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa
     3361 ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag atatggctga
     3421 aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag gatatggacg
     3481 gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac gggccgatga
     3541 tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag accaaccgct
     3601 aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc gtgggtttac
     3661 tgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt
     3721 aacagaaggt attgtcgtag attcaggttt aacatttggg aagttgcgtt tttccgcatt
     3781 acgccgagaa gtacggaagc aaaatgagga tggaacaatt ggtaatgagg taaaagaacg
     3841 aacctataat ttaaaatcct ctgcacaagg gcgaatgatt caagttagta tcccagctaa
     3901 tgtaccctta cgcgagtttg cttatgatgc agaggtagag ttggtcaatc caatcgtgga
     3961 taccgttgcc aattatgttt ttcgagaagg aactaccgtt aattggttca ttaaggcgga
     4021 tgatttagtc ttgaagcggc agccgaatca aggaaatcct acgaaccaaa atgaaagaaa
     4081 gaaataggtg aagattatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaata
     4141 tggaaaaggc gagctgactg aggaacaact cgttaaagca ttaacctctg atgaaaaaat
     4201 gtttctgatt atacatcaag gtttacttgg agaaaatagc gacagtgaag aagactctga
     4261 tgtactagac tggttaggag aagaagagtc tttctttata gttgtcgaag taaatgaaaa
     4321 cttatgtcgg caggcagaat ccattttgga agaaattggt gtggagatgc cggatgctat
     4381 tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt agcagtaaat
     4441 gactagcata actttttgaa attgcctaat gaaatcctat tgggcttttt cgattggagg
     4501 tggaaaccat caaaatgtac aaaggacatc gcatacgcgc aggagatcag cacttggttt
     4561 atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt
     4621 ttcaagagtt tagacaattt gatatttcta agctatcact ttctactatt gaaatcgttt
     4681 ggtctataaa agatttagta tgtctgcttg gaagtctcgc tttttcagga gctatgatat
     4741 tgctttatat tcactttttt cttgatcatt ggcgaagctt gtggcatcga caaaagctag
     4801 ctcggatgat tctagagaac cattggtatg aagtgaaaca aactcaaagt gaaggctttt
     4861 tcaaagattt gaatagtagc cgaaccagag agactatcag ttacttcccc aaaatctgtt
     4921 atcgaatgaa agatggatta ctgtctattc gcgttcaaat ttcacttggg aaataccaag
     4981 atcagctttt aaaattggaa aagaagttag aaagcggact gtattgtgag ttagtagaaa
     5041 aggaactcaa agactcctat gtggagtata ctttactgta tgatatgatc gccaatcgaa
     5101 ttgggataga cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa aatcaagtat
     5161 gggcttatga ttctttaccc catatgttaa tcgctggtgg cacgggtgga gggaagacct
     5221 atttcctcct caccattatc gaagcactat tgaagtcaga tgcagaattg tttatcctcg
     5281 atcccaaaaa tgcagattta gctgatttag gtacggtgat gcctcatgtt tattcacaaa
     5341 aggaagaaat ttctgcttgt gtggaagatt tctatgaacg tatgatggct cgtagcaagg
     5401 caatgaaaga aatgtctaac tacaaaacag gagagaatta tgcctatctt ggacttccac
     5461 ccaacttttt aatctttgat gaatacgtgg cttatatgga gatgttaacg acgaaagaaa
     5521 gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct
     5581 ttctgatttt ggcttgccaa aggccggatg cgaaatattt aggagacgga atccgcgatc
     5641 aattcaactt tcgcgtggct ttgggtcgta tgagtgaact cggatattcc atgatgtttg
     5701 gggaggtcga aaaaaacttc tttatgaagc acatcaaagg tcgaggttat gtggatacag
     5761 gtggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttag
     5821 attcaattag taaattagaa aagatgaatg attgagtgat ctt
//
LOCUS       DAHUWM010000163         5861 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHUWM010000163 DAHUWM010000000
VERSION     DAHUWM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.167"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(192..662)
                     /locus_tag="NK788_002224"
     CDS             complement(192..662)
                     /locus_tag="NK788_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCH6770539.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="NK788_002225"
     CDS             992..2632
                     /locus_tag="NK788_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCH6770540.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="NK788_002226"
     CDS             2786..4108
                     /locus_tag="NK788_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCH6770541.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="NK788_002227"
     CDS             complement(4176..4610)
                     /locus_tag="NK788_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770542.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5512)
                     /locus_tag="NK788_002228"
                     /pseudo
     CDS             complement(4675..5512)
                     /locus_tag="NK788_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         1753 a         1168 c         1078 g         1862 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     5101 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5161 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5221 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5281 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5341 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5401 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5461 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5521 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5581 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5641 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5701 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5761 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
     5821 agcttctcat gtttgttgaa tattgtgact tgtgaccgag g
//
LOCUS       DAHUWM010000164         5790 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHUWM010000164 DAHUWM010000000
VERSION     DAHUWM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.168"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(99..1229)
                     /locus_tag="NK788_002229"
     CDS             complement(99..1229)
                     /locus_tag="NK788_002229"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCH6770543.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="NK788_002230"
     CDS             complement(1877..3079)
                     /locus_tag="NK788_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6770544.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="NK788_002231"
     CDS             complement(3139..3342)
                     /locus_tag="NK788_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCH6770545.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="NK788_002232"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="NK788_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="NK788_002233"
     CDS             4879..5691
                     /locus_tag="NK788_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770546.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAHUWM010000165         5722 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHUWM010000165 DAHUWM010000000
VERSION     DAHUWM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.169"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(217..999)
                     /locus_tag="NK788_002234"
     CDS             complement(217..999)
                     /locus_tag="NK788_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770547.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1064..1900)
                     /locus_tag="NK788_002235"
     CDS             complement(1064..1900)
                     /locus_tag="NK788_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6770548.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1918..3327)
                     /locus_tag="NK788_002236"
     CDS             complement(1918..3327)
                     /locus_tag="NK788_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6770549.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3341..5164)
                     /locus_tag="NK788_002237"
     CDS             complement(3341..5164)
                     /locus_tag="NK788_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6770550.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5444..5671)
                     /locus_tag="NK788_002238"
     CDS             complement(5444..5671)
                     /locus_tag="NK788_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770551.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1746 a         1251 c          942 g         1783 t
ORIGIN      
        1 ttttcactaa aatcactacc tgaaacagtc taaaacactt atatatcaac gtttaatgaa
       61 actatctccg tttcatttca gatacaaata aatatagtcg ggctccaaat ccgaaaaaaa
      121 atacccccag tagcttatcc taagctactg gagatgtata agtacggtca tataacaacg
      181 gggtagggta aataaatgaa aaagatggaa gaatcattat tcaataactt ttttaaatac
      241 ttcgttgtaa tcagttacct gtccgttctt aaataccaca gaataatctt ttttcccatt
      301 ttcaagtaac cgaagtggaa tgtctttacc aacttgctct gtcactgcat tactgatacc
      361 tgttagatta gctaataaat ctgataaaga ttgagcatct gtagaaatct ctgtatcagt
      421 atttatcgtg attcctttat cttcatttac aagcttccaa gtaggtggta tagctgtgta
      481 tgtttcgatg aatgaatcaa ttctttcatc tatcgtcttc ccagtatcta cttctgtctc
      541 attactctta gatgaatcga ttctttcatc catcgtctgc tccgtaactg cttctgtctc
      601 attattctca ccggaaactc tcttcacacc caaacttata tattgtccat ctttagtgtg
      661 catagttaaa attcccacgc ttttactaat aaattcacta taaccagttc tttcaataga
      721 gacttcaatg tatttcacac tatcatactc aaaagaaaaa tagacatgta aaagcatttt
      781 gttgtttaca tctacataat aatcctttga ctctacttga gaccttccta tacggaatac
      841 agctttactg tctgatatct ctataccagc atccttatta ttaatctgcc aatctccaag
      901 tagctcttct ggcaagctag tagtttcttg tgaacacttt accacttcac tggtagaggt
      961 cgaaacattc acctccgcac tgttagaaga attttccaag gaatcaggag ccttagcaca
     1021 accaacaagt ctttatatat tgtgcagccg atattttctt cattcagtac acccttgaaa
     1081 tatgcagtgt tagataaagc agttcgtcct gatttgctga ggcacccatc tcttcttgaa
     1141 cgtactcctt gattttcaag gccgcccgat actctttcgg aaaacgctgc ttcactgaag
     1201 ccagcaaaaa atcatccttc tccggcaact cttccttctt aatcattcgg gcaataaaaa
     1261 actgtaaatg cgtcacaaat cttaagtaaa gcatcgaatc tttatcaacc tttttttcgg
     1321 gaaacagata tcctagcata gccaagatat cctttagtgc tcttaccgag agcatggttt
     1381 cttccatatt tgcttgttca ctttgggcat ttacaatgtg aaacgcaatg tttccagcct
     1441 cctcttctgg caactgaaca ttcatttttt tgttgatcaa ttggatagca tagcagccga
     1501 tctcaaattc ttttgggtaa tacttttgaa tttcaatcag caggcgattt tgaaaaacaa
     1561 tgcctttttg actgcgttca atggcatgct tcaagtgatc aaccaaggta aaaaatagac
     1621 ttttattcag cttaaagtca aacttggcgt tggcataatc gataattcgc ttgacgcaaa
     1681 taattagatc atccggagca gcctcaaaca gtttaatgta atcctttttt agagcatcag
     1741 aaagtgaatc taatttaaat actttttgga ttttgtcagc cggaaccaaa tcgcctttct
     1801 ttagttgaaa tccaatccct ttccccatca aaattacttc tttgccttgc ttatctaagc
     1861 aaagaattaa actgttgttt aacgttttaa ttactctcat tagattcacc gttctctcta
     1921 agctaagcca tttcttgcga ttactttttg ataccaaaag aagctttctt tttttattcg
     1981 ttttaattct ttaagattct catcggttcg gtcaacatat ataaaaccat aacgcttatt
     2041 gaagccttga tgggtactaa caacatcaat cgctgaccaa ggtaaataac caaaaacatc
     2101 tacaccatct gttaaagctg cttgaatagc cagaatatgt ttggctagat aatcaattcg
     2161 atcctggtca ttaattcgtc tatcttcctc aactatgtcg ggtgccccat agccattttc
     2221 agtaattaaa ataggtaaat catagcgatc gtacaatttc ctcaatgtta atcgtagtcc
     2281 agtagggtca actacccagc catatggcgt tttatctaca taaggatttt cagctgcccg
     2341 gtagacacct tcttctccca acatgatctg ttgatccccc gctctagcgg aaacatctga
     2401 tccgtcattt ttgctggcag cgatggtggc agttgaataa taattaatcg caataaaatc
     2461 gggtttaccg gaggataaaa ttgcctgatc ctctgcctca attttaggat ataatccacg
     2521 atcattcaaa tagttttcaa acaaataatt gtattttccc cgaactgcta catctaagaa
     2581 agaccagcct ctcaatgttt cccaattatg ggctgcaatt gcatcttctg gactggaggt
     2641 agcttgatac atagccgtta aattgattgc tggaccgatt tttcctttaa gattcaactg
     2701 atgatacaag tgaattactc tagcttgagc taataaaaca tggtgattaa tttgaaagag
     2761 ttcttttttt gaaggcagct ctccacttgg tggcaaacca atcgctccag gatgaaggat
     2821 catcgtgttt tgctcattaa ttgtcagcca atagttcacc ttgtcaccat actcagtaag
     2881 caatacctgt acatagtcca caaaataatc gatagttgct cgatttaacc aaccgccctg
     2941 ctcctctaga aaataaggta aatcgaaatg gtacatggtg acaatcggtt cgattccctg
     3001 attcaggcat tcatcaatta cttgatgata aaaagaaatt cccttttgat tggttttatt
     3061 attttcaata tccataattc gcgtccaagc gatggaaaag cgaaaagctt ttaagcctaa
     3121 ttctttaaac agctgtatat cttctttata gtgatggtaa aaatccgagg caacttcaaa
     3181 attagctacc ccttctgtat gctgatactt gtcaataata gacggttgtt tcccatcctc
     3241 ctgataggca ccctctgcct ggtaggcact aatggaggca ccccataaaa acttctctgg
     3301 aaatgctgaa agctgtttgt attgcacaaa aatcctctcc ttaactaatt tgcatcaatg
     3361 gttcgtgttc ctttaagctt ccgcttgctt cactaattcc tgcgtaattt tcactgttgg
     3421 tcactaccac aatcactgta tcatcgtagc cagcgtttct aatggcagcg aaatcggcgt
     3481 tgattaataa ttgtcctgct gacacctgat ctcctgcttt tacaaatact tcaaagggtt
     3541 ccccttttaa attcacggtg tcgatcccca catggatcaa taattcaata ccttctttag
     3601 aacgtagacc aatcgcatgt ttagattcta aaatagtaat gacttctccc tcaacaggtg
     3661 aataaatgct gttttcttct ggatgaatgg ctacgccctt tcccaatagt cccttagaaa
     3721 agacagcatc gttcacttca gaaataggac acacggttcc ttttaccgga ttttcaacaa
     3781 tgactgcttc ggctattgcc gtttgctctg attcatcaga agtatcgtca aatccaatta
     3841 gataagcaat gataaaggtt gcaacaaagg cgattacaca cgtaataatt gcgtgaacaa
     3901 tattcattgg attttctcca ataaaggctg gtaaggctgc taagccaggg gaaacaaagg
     3961 catatctgac taaaccgctc aagccagcat acaaaccagc aattgcacca ccaatcatgg
     4021 aagcaactaa tggttttttc agcttcaagg ttactccata gagtgaaggc tcagtaattc
     4081 ccattaaagc agtgatccca gtggacacag ctagctgctt caactctttg tttttagttt
     4141 tcgcggctac agcaaaggta gcacctgatt gagcaatatt agaagccagc attcctggcc
     4201 cattgatcat ttcagaaccc gtactgctca attgcatagt agcgattggc gtcattgccc
     4261 aagctgtacc cgtaaccacc aaaaatggct gcaaaccacc catcaaaaat ggaattaacc
     4321 aagctgcttt gccgttaatc cattcagccc ccattgctat aagatcgttc agcaacgttc
     4381 caaacggtcc aatgacaacc aacgctaaag gccccataat taaaaatacc agcatcggct
     4441 tagtaaagaa tttaataaac gaaggcgaaa ttcgatccgc aaagggctct acataggaca
     4501 tgatccaaac agtaaagata atcggtaaaa ctgatcccgc ataatctgca gctaataccg
     4561 gaattcctag aaaagaaacg gcatcgccag tagaaaaaag ctgtgaaata tttggatgaa
     4621 gcaaaatgcc agcaacagta atcgccaaaa ttgggttgac atcaaacttt atggctgccc
     4681 cataggctaa tagtactggc ataaaataaa aggctgcatc agcaatagta ttcaaaatga
     4741 tgtaagtttg actactgtct gatagtacac cagtcaaagt caagacggcc aaaatggctt
     4801 ttaccatccc ggcacctgtt atagctggaa tcatcggtgt aaaggtggta gaaatgacac
     4861 taatcaatct tgaaataata ctttcttgat tgtctgaaga ggggcccttt ttaattgaag
     4921 ttcccacttc cttttgaatt tccttgaagg ctgtttggac ttcattgcca atcaccactt
     4981 ggtattgtcc accgctatcc accacactaa ttacaccaag tgttttttct aaggctttcg
     5041 tatcggcttt ccttttatct ttgagttcaa aacgcagtct ggtggcacaa tgggttaagc
     5101 tgaccacatt ttcttttccc ccaacctgtt gcaatacatt tcttcctact tctctgtaat
     5161 ccattattct ctctccttaa agcagtaaaa aagaccaagt caaaccacgc cccaatcaac
     5221 attgaagtgt tagtttgtct tggtcttgcc tgcataatca gtaacatgcc aataacgtta
     5281 ttttgctgta atccaataat agcacattat gatagcgcaa tcaacaggga atttaatttt
     5341 ttatcttttc gtttgaccgt atagggtaat ataacagtta gatcacatca actagggtat
     5401 atctgcgtat acttactgct aaaataagta tatctaatat taactatacc agtaaaacca
     5461 ttcctacata accagcggga aattcccaaa atcgtgaaag catcaaaaat gcatataacg
     5521 gatcacacaa cttcaacaat tctgctcttg ggaaaaattg atgaatttgg tgagtaaagg
     5581 atggaataaa cagcattcca acaatatagg caacaatagt gatacttgtt tttttggtaa
     5641 aaatcagtaa gcaatagcca atttcaatca ttccgagata taaaggtatc gatagaatga
     5701 tttgggctag tattcttaaa aa
//
LOCUS       DAHUWM010000166         5719 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHUWM010000166 DAHUWM010000000
VERSION     DAHUWM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.170"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..3105)
                     /locus_tag="NK788_002239"
     CDS             complement(<1..3105)
                     /locus_tag="NK788_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCH6770552.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRK"
     gene            complement(3119..4336)
                     /locus_tag="NK788_002240"
     CDS             complement(3119..4336)
                     /locus_tag="NK788_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCH6770553.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4326..>5719)
                     /locus_tag="NK788_002241"
     CDS             complement(4326..>5719)
                     /locus_tag="NK788_002241"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCH6770554.1"
                     /translation="ELLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQL
                     NDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHD
                     GDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMG
                     SGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKD
                     WPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHL
                     KETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLK
                     KNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKE
                     NDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEA
                     WLQGALEVFKHEQ"
BASE COUNT         1547 a         1217 c          970 g         1985 t
ORIGIN      
        1 tttacgccat gctcgcttca tttcttgtgg gtatttaaat ggtttcgctt ctggatgttt
       61 ggctttatat ccttcgtaat ccttactgtt gataattgct ttcatattag gaattggatc
      121 ttcacctggc atatattgca gattagaagc atgtaattct ttctcagaaa caaaccactc
      181 attggaaaag gcatgaatca aattatcact tgcttgtgta aaatagcgac gtttaatgac
      241 aaagatatct tcattactat ctaaagtccc cacatcaaat gcatttaaaa tttgtcggta
      301 aacttctttg actccttctg ttttattcaa tttagccaaa gctttttcaa tttcttctcg
      361 tgcatctgaa ctattcgcag cataagttcc tagtaattga tcaatataag tagaatcaat
      421 atcatacaac ttagtggtat tttcactata aaattcgatc gttgaaaaat cttctccctc
      481 tttttctggt tcttcctcca tcaatgaacc cttcacggtc tcgtacacgc caatttgttc
      541 ttccaaaagt tgaatttgat tgttcaaagt tgaagattgc accatgtcat cattgtaatc
      601 atcataagtc actaacgatt catacgcacg attcaattct tgaaatgctt tgacgtactc
      661 gatacggatt tcaagtggcg tatgttcatc aatggattcc atggatgaaa cctcttttaa
      721 cttaccatat gcttgcttga atcttttttt ggattcttgg taacttggat aaatcaaacc
      781 cgattcttct ttagcttctg aatacagttt tgtcgcatcc tcgacattct tctccattgt
      841 cgctggcttt cggaaagtaa caattagtcc ttttgttttt tcaggatacg tacggttcgt
      901 acgtgaaaag gcttggatca atcctgcata ctctagatta cgatccacaa atagggtttg
      961 aatcgtaggg gcgtcaaaac ctgtcaatag acgatccact acgattacta aatcgatttg
     1021 tttcccaaat tgtttgaatt ccgctctttt tcgtgccaaa cgattattga tgtctccgtt
     1081 ataacgctca atgtctgcta gtgaccaggc agtatcataa tactgattat attcctcaat
     1141 aattttttgc atttcttctt gttgggcact tgaatctcgg ctattttctt ctaatgaata
     1201 ggtaatagcg attcgtggga aatcaggatc ctccatcgtt cgtccttcac ggataggctg
     1261 attgggaaac tcttttatta accaatcaga ttctttcgtc atctctttga tggcccgata
     1321 atactttttg gccatatcaa tggaactcgt ggttaagatc gcagacttcg ttggacggcc
     1381 atttcgaaaa tcaaattttg tataggcatt gtctggacgg aacattttat gaatgacttt
     1441 ttggatatgt tcctctgctt caaaaacagt agggtccaaa taactttctt ttttgacaga
     1501 ctccatttga tcgatcatac gattgatctg ttccggtgaa tactctgcat atgtttctac
     1561 ttcttttaat ttccgataga ttttattctc taatgaagtc ggctccacgg tgttttcatg
     1621 ctctacttga aatcctaaaa ccgagccatc ttctaatgca ttcttgatgg tataggtgtg
     1681 caagacctct ccatactgat catgggtcgt acgtgccaat tgccctttgg cttgtttccg
     1741 attctcaggg aaaatcggtg tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt
     1801 tttgatttct tccatatttt cagcacttaa cgctcgatga cactcatcaa ctacaaacac
     1861 gatatgttgc cccattaatt tttggaagcg attggttcct tttttctctt cctgtttctt
     1921 tgcatatttc aacgccgcat ctagtttttg acgggttgta atgatcacaa cattcgcatt
     1981 ggcatctgcc aacagagttt cacttaactc tttggcactt cctgttccca cgatcaagct
     2041 attcgctttg gcattaccag aagaaacccc agtattaaac tccgaagcaa atttcgtaaa
     2101 ctctgtggtg gtctgattat ctaaatcttt gcggtccact aacatgatcg ttcgatcgat
     2161 ccccgatttt cgtgctaaaa gcttagtaga tacaaaacta gtcaacgtct ttcctgaacc
     2221 agtcgcatgc cacacatacc ctgattgatg tttatttgcg gcaataaaca aggcttgaat
     2281 cgcatgcact tgataaggat gtaacaccat gagggtttta ttgtcttgat cttcactgac
     2341 aatcgtataa tccgcaatca agcgatgggc atctgggata tttagcactt gtttgcaaaa
     2401 ctcatataag ttttctacct ttttattatc tttcgttcgc caactaaata aaaacttagg
     2461 atgcaaatcc ttcggtaagg catttgcaaa ataccgagtc gtttgttcat tggaaaccac
     2521 aaacaactgt aaggtcgaaa agatattgtt ccgaaacatg ccttcttcag catatttttt
     2581 gatttgatta aacgcctgat aaaaaccatc ttttgcagtt acttgtttta gctcgatttg
     2641 gataatcggt aagccattga tcaataaggt gacgtcaaaa cgtcgatccc gcgcttctat
     2701 gttacttcca cgttttgcaa tttgatggac cacttcataa gtagagattc ctccaccaat
     2761 gtcttgattg gaataaagga taagtgagac agaacccaat tgcggatttt cccgttcaat
     2821 cgtgatccga gccatcccat tttcaccttt taaccatttc gcagcatcaa atggggtttt
     2881 cgtccgtagc aataattcgg ttttgatcgt ttcaaactct ttatcggtca aaggagaatc
     2941 attcagttct gcttgattat ttgagatgat tttttgacgt aaattgctcc aaagatcttc
     3001 ttctgatttt agatccggac gatacgtcca ctgattatgt ccttccccga ggacacgaat
     3061 caactgttct tcgacttccg cttcctcacg gtgactaatt ttcattcttc ttccctccct
     3121 aaacaaacat cttttgcaag aagccttttt tcgtttcctt cagcaaatct aacttacgtt
     3181 gatgaagagc gatcgtatca tctagctgct tgaagaaggc accaattttt tgttgttcat
     3241 ttatatccgg tggaaactga aatgtccttt gttctatacc acctttagtc acatgtacca
     3301 ttgtcgttcc gttagttgat tctttaattt tctctttatc agtcaatagc caagtatata
     3361 gatactgttt gtccaattcc ttagataaaa atttaagttt ccaaatatga taatggtaga
     3421 ttacacgttc ttcattccac atttcaggac cgaagcttgt tgcccataaa tataagagat
     3481 ctcctttatt agcatacttg tcttcctcta attctaagtt tgaataatac catctatcat
     3541 tagtattaaa attacctact cttaaaaccc tatattttcc agaatcaagt aattcttgtt
     3601 gtttaaaagc tctaccattc aaaaaaacta ccacttcccc caacttccgc tcttcccaat
     3661 ctcccgtaaa tcctggaaaa cgaacttctg gcacttttgc tccgttttta gggaacattt
     3721 tttgtaagaa tccttttttc gtttccttca gcaaatctaa ttcgtgctgg tgaagcatga
     3781 tggtgtgatc aagttgtttg aaaaatgaac cgatcttgat ctgttcgtca taagttggaa
     3841 gtgagatagg tgtcttttta ccactatcaa ttgtataggt tccgacggtt ccaatgcctg
     3901 caacgtcacg gatatacttc tgaattttgt tatttatgaa cgagtagtaa aagaagtttc
     3961 cgtcaatttt atgctcattc ttgaaccaaa tgatgtttcc atccttaaag taaattgggt
     4021 tatcatcagt gattaataag gggacaccta ttgatccgac accagttaca agcaagtctc
     4081 cttgagaaac tttacctgaa tttttcgaat attcattata tttttcttct gaaatgtaaa
     4141 gataatcgga tggttcctca ttttttgcag ctgagacaat atcacgagca cgtaaaaatc
     4201 taatacctga gttagtccaa tctgattgat gaattcgctt cacagaagta acatccatca
     4261 tatctcctaa cttacgctct tcccaatctt ccgtaaaacc aggaaaccgg atttctggtc
     4321 gtgtatcatt gctcatgttt gaacacctcc agcgcacctt gtaaccacgc ttcattttca
     4381 ggtgttgttt gcagggaaga gatagcttct aaaagttctt tttctagcac ttgctgttct
     4441 tgtcgtatct tcttgatgtc atttccgaca tgaaccatat caattggttc ttcttcttcg
     4501 aaagtatcga catagcgtgg gatatttaag ttatagtcat tttctttgat ttcatcaaag
     4561 gttgccaaat gagcgtattt ctcgacatct tttctttcag cgtaggtttc gaggattttt
     4621 tgaatgtttt cttccgataa tttattctga tttttccctt taacaaattc acgactagca
     4681 tcaatgaata atacatctcg agtttgacga ttcttcttca atacaatcac cgtcgttgga
     4741 atcgatgtac caaagaacaa gttcgcaggc ataccgatca cagcataaat actgccgtct
     4801 tctaataatt tttgacgaat gaccccttct gcagcaccac ggaataaaac cccgtgtggc
     4861 aagacgatag ccatagtccc tgtttccttc aaatgataga aaccatgaag aagaaacgca
     4921 aaatctgctt tggattttgg cgctaatttc ccgtagcgat taaaccgaga atcatctaag
     4981 aaggtcgtat ctgctgacca attagccgaa taaggtggat tcatcactac tgcatcaaat
     5041 gtgtaaggtt catctgttgg ccagtcctta ttcaacgtat ccccattgcg gagattcatt
     5101 tcttcggcat ctaccccatg taaaatcaag ttcatttttg ctagattgta tgtcgttgta
     5161 tttagttctt gcccatgata tttaacgtta tctggatgag ttaagtagtt acgtacattt
     5221 aacatcaagg aacctgaacc catggttggg tcaaagacac taaagaatcg tcgttctttt
     5281 tgatccaacg tcacgatttg tgccatcata tcagaaacca tatgaggcgt gtaaaattct
     5341 ccggctttct tccctgcttc tgaagcaaat tgactgatca gaaattcata cgcatccccg
     5401 atcacatccc catcgtgacc taaaacttcc acgtcattca atttcttgat aacttcggta
     5461 atcgtcacat ttcgttgttg ctcatctgtt cctagttttt ttgattggag atccacatca
     5521 tcaaataacc cattaaattg attgtaggta gatgccaatt gcacaaaggc tttgtttaga
     5581 tcgttcaatt ggaacgttgc ttgttttgct tgatccgcta agacgttgaa aagatactct
     5641 ggtgagatcg aataccccaa aatatcaacg attgtggcaa tcaggtcttc ttttgattct
     5701 tcatctgaaa gtaattcac
//
LOCUS       DAHUWM010000167         5704 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHUWM010000167 DAHUWM010000000
VERSION     DAHUWM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.171"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(95..1216)
                     /gene="mnmA"
                     /locus_tag="NK788_002242"
     CDS             complement(95..1216)
                     /gene="mnmA"
                     /locus_tag="NK788_002242"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCH6770555.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            1620..2219
                     /locus_tag="NK788_002243"
     CDS             1620..2219
                     /locus_tag="NK788_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCH6770556.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2357..3643)
                     /gene="efmA"
                     /locus_tag="NK788_002244"
     CDS             complement(2357..3643)
                     /gene="efmA"
                     /locus_tag="NK788_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCH6770557.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3914..4258)
                     /locus_tag="NK788_002245"
     CDS             complement(3914..4258)
                     /locus_tag="NK788_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCH6770558.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(4395..5540)
                     /locus_tag="NK788_002246"
     CDS             complement(4395..5540)
                     /locus_tag="NK788_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCH6770559.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT         1680 a         1233 c          992 g         1799 t
ORIGIN      
        1 aaaattaatt ttattgcaaa aaaacagcta tgctatccaa aagaaaaggt gtttcttttg
       61 ttagccatag ctgttttgat tcatttaata tcgcttaaac gtattgtaaa acttttgttt
      121 cttgatatgc gtggtcgatc aaaccaccac ccagacattc cattccatca taaaatacaa
      181 ctgcttgtcc aggagtgatt gcacgtacag gttcgtcgaa tacgacttct gcacgattat
      241 catccaataa gcgaactgtc acaggaacgt cttgctgacg ataacggaat ttcgctgtac
      301 acttgaattc ttttggcatt ggttcattcg tcgtaaagtg gatttcactt gcatccaagc
      361 ttgtggcata caatgcagga tgatggaacc cttgccccac atataatgtg tttgtcgcta
      421 gatcttttcc tacaacaaac cagggttctt gcgaatctcc tccgccaccg atccctagtc
      481 cttgacgttg accaatcgta taatacatca agccagcatg ttgtcctttg acttcaccat
      541 caagagtgac catatttcct ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt
      601 tcttttcacc aataaagcag attcccgttg aatctttttt cttagcagtc gcaaggcctg
      661 cgcgttcagc aattgcccgt acttctgatt tttccatgcc gcctagaggg aacatcgttt
      721 ttgaaagctg ttcttgtgat aattggctaa ggaagtaagt ttgatcttta ttattgtcta
      781 ctccacgaag catgtggacc acgccgtttt catcgcgagt gacttgcgca taatgtccag
      841 tagctacata atcggcgcca agatccattg cataatccaa gaatgctttg aatttgattt
      901 ctttgttgca catcacgtct gggttaggtg tacgtcctgc gcggtattcg gctaggaaat
      961 attcaaatac acgatcccaa tattcttttt caaaattcac agagtaataa ggaatcccga
     1021 tttgagcagc tactttcgct acatccttat aatcttccgt ggccgtgcat acaccgtttt
     1081 cgtccgtgtc atcccaattt ttcatgaata ttccgatcac gtcgtatcct tgttccttta
     1141 acaaaagtgc tgttacagat gagtcgacac cgccgctcat ccctacgaca acgcgtgttt
     1201 tgctgttgtc tgtcatgata tcaccatcat ttcaattaga ttctgaaaca tctacgagtt
     1261 gaaggtcgca acttttcagt attttacatt atacgtcata tttctgaaaa tttcatccct
     1321 taattatcaa gtcgtaaaaa tactgttcta ctggacctat tcagtacaca aacatgatag
     1381 tcaaccaaaa aatcactcat cttttgagca ttcgatataa aaaattggca tactcataaa
     1441 aagggccagc ctttcggcaa taaggaataa ttttataaat tgtgaaaaac tatttttcaa
     1501 aattgcttct tatcgcttaa agctgcacgt ctcatttaca accttttctt tttagatgtt
     1561 atgagttgct cagataagtg attttgttta ctctaagaaa aaagggaggc aaaagataca
     1621 tgaattactt tatcagtgat atgcactttt tccatgaacg cttattagga aaaaacgact
     1681 ttgctccgcg gctctttgca aatgtggatg aaatgaacca acagttgatc actagctgga
     1741 atgcagtaat aaaagaaacc gaccatgttt atcatctagg cgaccttgct atgcatccgc
     1801 aatatgaaaa agcaaatgca gaaattttgg aactggtcac tcaactgcaa ggaacaatcc
     1861 acttgatcaa agggaaccat gacagtcgcg cttttttccg ttatttagaa aaaaatgatc
     1921 ctaggcttac tgacggaagt cccaaatttt atttttatga tgtaggtgcc atcgttaaat
     1981 tcgaccatca acaatattac ttgactcatt atccgatgtt gctcgggcct aacgacacta
     2041 tccgcaatct tcatggacat atccatcatt acagcgtacc tatcgagaac gatgtcaatg
     2101 taggcgtaga cgcaccggaa agagaactct taaaggaaaa actaccattt ggcactccgt
     2161 tatctgagca acaaatcaaa gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa
     2221 aaaactgccc tatatcaagt attaaacaaa taaagataat tactcatatt tttcatttga
     2281 gtaggcttat tgccacagga acattaggat cagaaatgga gttgtaacaa gacttctgtc
     2341 acaactccat tccatactat tcgtcttttt gctcatggtg ttcttttacc agttttttct
     2401 gtaattcttt atcataattt cgggcagctg acgttaaaaa taaaacgatt ccgcataata
     2461 aaatcccgat tccggaaaac aagaacattt tttctacccc taaacgatca gctaatggtc
     2521 ctgcaaataa taatccaatt ggacctgtca ggttcatcaa agagttgaag ttccctaaga
     2581 cacgacctaa aacattcgat tcataactct gctggatcat tgccatcagc agtgtattga
     2641 aatatggcgt tgcacaacct gctcctgcat tcaagataag aaagtacaag aacccttgac
     2701 tatcgtttgg aaccaatccg cttgcaccga tcgtcaggcc cataatgatg taggctataa
     2761 taataggttt cattcggtct ttccatgttc ctataaaact gatcagtgca ccgcctaaga
     2821 gcattccgac tgcgtagact acttccactg ctcccgcctg tccaacggaa ccaccaaagt
     2881 aatctagcgt catcaatgga tacagactga ttgctggcat gaacaacagc atcacaaatg
     2941 caccattgat cgttatgtac cataaccctt tattatccat caattgctgc agcccaaatt
     3001 ttgcatcttt aaaaatagta agcggaactt ccaatatttc aggagaaact tttgggatct
     3061 tcacaaaaat caataagccg acaccaaaaa ctgcccccaa tacatctaat aaaataaggt
     3121 aattgacagg aacgactgaa aacaaagccg cacctagagc tggcgcaatg atgaaattcg
     3181 ctgattggac cattcctaac tgtccattcg tccttgttat gtgagaagat ggaacgattg
     3241 tcggcataat cgactggatc gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca
     3301 aagacacaaa aactagccaa atcgggaagg attctgaaat cgttccgact actgctaaaa
     3361 taagcgcgaa aatcgctaca ataatatccg tgacaatcaa aagagctttt ttatcccagc
     3421 gatccactag cggtcctaca aatgggctta gtaaaaccat gggaatcatt cctagtaaag
     3481 tagcaaaact caaaattgtc gccgaaccgg tttctcttgt caaataccag atgatcgcat
     3541 attggacgac catactagtg attcctgaca aaaactgtcc cgataaaaag aacaaataat
     3601 ttctcttcca attcgttaaa acgaccgact gttcattttc cataaacatc cctcttttct
     3661 ttttcatttt gtatagcgtt tgcacaacaa tcattatacg ggattctttt ttcaaaaaat
     3721 acctttttcc ctaaataaca atcgtttcaa tttaaatttc tttacttaga aacaataaat
     3781 cagcaaaata ttaaaaataa caacatgtgt taaatttaaa aataaaaaag acaactaatt
     3841 tgcgtcgtcc ttacaaagtt agtcatctaa caataggccg tttcaaaact agttgtcttt
     3901 ttatatccac agatcattcg actttttcga ataacccacg gctaatcatc ccatccatca
     3961 gaaaataaaa tttatcttgg ctcattttct gttttggatc tttgaaaata ttgactgctt
     4021 tcaatccatt tgctgtagtg atcgacattt tcaatgtttg aatatctttt gatacagtga
     4081 tttttaattt gaatccttct ttgctttgcg gcgtggcatc taatggtcgg atcaattgat
     4141 aattgcctcc gtttgtttct gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag
     4201 gatgcaaacg atagctaatt ggtgcgccca gtaccgttgc tgttgtttca aatgccatga
     4261 ttttcacctc gataaaatta gtttctcttt attataaaag tttatcctga tttttactag
     4321 gtttttcagt taagttccct aaaaaatgaa ggcgttctct cagacatctg ttcatataag
     4381 aatgacttca ctagttattt tttgacagca cgaactaatt gatcaaggaa atactggatg
     4441 tcgtcttcgg tatttcccaa accaaagctg atacggatgg attcttttag tatcggtgta
     4501 tcttctccat acattgcttc caatacgtgt gaaggctcga tatttcctgc tgtacaagct
     4561 gaaccagtag aaatagcgac cccattaaga tcaagcttca tcagtagcaa atcatttgct
     4621 tcgccaatta agcgaagatt caacacatga gggagtttat tcgttaaatc accatttact
     4681 tgataaggga tttcgtttgc ttctaatgta tcaagaatca attgctggaa tcctttatat
     4741 ttgttgttac gtgcttcacg ttcttcaggt gacagcagtt caactgcttt tgccataccc
     4801 cagatccctg ctaaattttc tgtacctgca cgccgctttt cttcctgctc cccgcctaaa
     4861 agcaatgcag ggatatttac tccgtctcgt ttatataaga aaccgacgcc ttttggccca
     4921 ttgattttat gagcagagat acttaataaa tcgattccta attcttctgg atggatcgtt
     4981 tggtttccgt acgcttgtac cgcatctgta tggaaataag ctggatgatc cgctaaaagt
     5041 tcaccgattt ctttgattgg catcaaattt ccgatttcat tgttcccgta catcacagag
     5101 accaagatcg tatcttctcg taacgctttt ttgacctctt cgatcgacag attccctttt
     5161 tcatctacag gcagatatgt aacgtcaaac ccctttgtct ctaaatattt catggtatta
     5221 agaacagcag gatgttcgat agctgtagtg attagatgtc tgccttcttt ttgacgcgag
     5281 aacgctgttt ctaagatagc tgtattatcc ccttctgttc ccccactgtt aaagatgatc
     5341 tcgtgaggtt ttgcttgcag actgtctgca atgatttgtc ttgctgtttc caatttttca
     5401 tgcgcgtggc gaccaaatcc atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt
     5461 gccgtcatcg cttcaattac ttgtggatgc atgggggtag ttgccgcatg atccagataa
     5521 atagttttca cttcattcat cccttccaaa ttattgctag gcacattcta gcataaaata
     5581 gcgaacaaac caaagaagag ctcatttttg tctagaggac gtttcatggg catagaaaaa
     5641 aggagctgtg acaaaagtct tgtcgcagct ccttgaagct gctcacttct gtcacaactt
     5701 cttt
//
LOCUS       DAHUWM010000168         5604 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHUWM010000168 DAHUWM010000000
VERSION     DAHUWM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.172"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(124..582)
                     /locus_tag="NK788_002247"
     CDS             complement(124..582)
                     /locus_tag="NK788_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCH6770560.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(595..1926)
                     /locus_tag="NK788_002248"
     CDS             complement(595..1926)
                     /locus_tag="NK788_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCH6770561.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1937..3379)
                     /locus_tag="NK788_002249"
     CDS             complement(1937..3379)
                     /locus_tag="NK788_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6770562.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3366..4130)
                     /locus_tag="NK788_002250"
     CDS             complement(3366..4130)
                     /locus_tag="NK788_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCH6770563.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4234..5109)
                     /locus_tag="NK788_002251"
     CDS             complement(4234..5109)
                     /locus_tag="NK788_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6770564.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5364..5448)
                     /locus_tag="NK788_002252"
     tRNA            complement(5364..5448)
                     /locus_tag="NK788_002252"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5412..5414),aa:Leu,seq:caa)
     gene            complement(5466..5537)
                     /locus_tag="NK788_002253"
     tRNA            complement(5466..5537)
                     /locus_tag="NK788_002253"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5503..5505),aa:Gln,seq:ttg)
BASE COUNT         1719 a         1184 c          838 g         1863 t
ORIGIN      
        1 aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg aaacatatca
       61 tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt ttttcatcgt
      121 acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt ttaataaatt
      181 tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg cttgctccaa
      241 cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt tactttcaac
      301 ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat ggatcagtat
      361 ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata tattatcgat
      421 tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt atccaactcc
      481 tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa tttgaccact
      541 agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc acctctactt
      601 taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt tttttgcgac
      661 tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt gataagaacc
      721 ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca tcaatccttt
      781 atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg caccaacata
      841 tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt gattaggaac
      901 gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat cgacgatata
      961 ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc ggcgtatttt
     1021 atcagccatt tctttggtat ttttctctcg tccatccatg accatattag cacgtgtata
     1081 ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta agtaattatt
     1141 cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg ttttttgcca
     1201 agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta gtttttcaat
     1261 aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat accagccaaa
     1321 gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt aattcactgc
     1381 aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta cattcggata
     1441 tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca aaatccatgc
     1501 tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga ttgagcgcat
     1561 cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat gtttaagtgg
     1621 aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa
     1681 atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa tatttgaaaa
     1741 accttttctt ttcagcataa atttaacaat caagtacata tccatatttc tttcttcatc
     1801 aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca aaatggcttg
     1861 aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct gttttttcat
     1921 catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata acataagtca
     1981 cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc ccgcttatcc
     2041 ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct acccgtaggc
     2101 gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct tgtagcattt
     2161 ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct tctaaggtta
     2221 cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt ctcttattga
     2281 tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt ggaagaatca
     2341 aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat gctccgaatg
     2401 taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg gcaaaaagtg
     2461 catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca ggctcagtga
     2521 tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc atctctttat
     2581 tttctttata agctgcttta tagatagcta gacatatagc tggtatacca aacatcatta
     2641 ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata aagcgtgtca
     2701 tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg taataaatat
     2761 acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa aacataaata
     2821 tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca atcccaattt
     2881 ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga atcatcacca
     2941 tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat ttttgttctt
     3001 taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca aaaatcccca
     3061 tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc gctaaaaaac
     3121 cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca actcctatag
     3181 caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt actgggatgt
     3241 tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc gctgcaactg
     3301 gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt tttattcctc
     3361 ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt cactttttga
     3421 gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac aaaacttgaa
     3481 ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat cgttttatct
     3541 tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt gatttgtcgt
     3601 tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc gacatgacga
     3661 acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt tgctaatgta
     3721 acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc taaatgagtt
     3781 ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg atacagttga
     3841 tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc tccgctttcg
     3901 ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa atggactcct
     3961 acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc cggcatattt
     4021 gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat cccataattc
     4081 acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat ttcattctcc
     4141 ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac tatgtgtcat
     4201 ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta gatgaattgc
     4261 caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga tgatatcttt
     4321 aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa aaaaaccaat
     4381 attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt tgatatgaac
     4441 cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg atttttctag
     4501 atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac tattaggctc
     4561 tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg accctaactc
     4621 ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat attcatttgg
     4681 ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt ctctttttcg
     4741 ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat ctaatggaga
     4801 ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa attcgattgg
     4861 aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta atacaaataa
     4921 tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc caatcccttt
     4981 ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat gattcagctt
     5041 tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac aacttgcaga
     5101 ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg acctaagaaa
     5161 gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt tacaaaaata
     5221 gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg ataatacttg
     5281 tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa ctctctataa
     5341 gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac gtcctcaacg
     5401 gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa atttaaaaat
     5461 aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac
     5521 cgcttggcta taccccaaga ataaagggcg aatgatggga atcgaaccca cgaatgccgg
     5581 agccacaatc cggtgcgtta acca
//
LOCUS       DAHUWM010000169         5594 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHUWM010000169 DAHUWM010000000
VERSION     DAHUWM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.173"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            66..767
                     /locus_tag="NK788_002254"
     CDS             66..767
                     /locus_tag="NK788_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCH6770565.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="NK788_002255"
     CDS             764..1885
                     /locus_tag="NK788_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCH6770566.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="NK788_002256"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="NK788_002256"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCH6770567.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="NK788_002257"
     CDS             3264..4172
                     /locus_tag="NK788_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCH6770568.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="NK788_002258"
     CDS             4207..4479
                     /locus_tag="NK788_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCH6770569.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="NK788_002259"
     CDS             4490..5536
                     /locus_tag="NK788_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCH6770570.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1819 a          970 c         1215 g         1590 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gata
//
LOCUS       DAHUWM010000170         5585 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHUWM010000170 DAHUWM010000000
VERSION     DAHUWM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.174"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(25..711)
                     /locus_tag="NK788_002260"
     CDS             complement(25..711)
                     /locus_tag="NK788_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770571.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(689..1183)
                     /locus_tag="NK788_002261"
     CDS             complement(689..1183)
                     /locus_tag="NK788_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCH6770572.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1203..2423)
                     /locus_tag="NK788_002262"
     CDS             complement(1203..2423)
                     /locus_tag="NK788_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770573.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2570..4405
                     /gene="lepA"
                     /locus_tag="NK788_002263"
     CDS             2570..4405
                     /gene="lepA"
                     /locus_tag="NK788_002263"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCH6770574.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4499..>5585)
                     /locus_tag="NK788_002264"
     CDS             complement(4499..>5585)
                     /locus_tag="NK788_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6770575.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1702 a         1125 c         1064 g         1694 t
ORIGIN      
        1 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
       61 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
      121 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
      181 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
      241 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
      301 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
      361 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
      421 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
      481 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
      541 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
      601 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
      661 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
      721 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
      781 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
      841 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
      901 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
      961 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     1021 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     1081 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     1141 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     1201 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     1261 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     1321 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     1381 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     1441 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
     1501 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
     1561 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
     1621 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
     1681 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca atagaacaga
     1741 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
     1801 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
     1861 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
     1921 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
     1981 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
     2041 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
     2101 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccccc cattttataa
     2161 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
     2221 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
     2281 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
     2341 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
     2401 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
     2461 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
     2521 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
     2581 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
     2641 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
     2701 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
     2761 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
     2821 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
     2881 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
     2941 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
     3001 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
     3061 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
     3121 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
     3181 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
     3241 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
     3301 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
     3361 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
     3421 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
     3481 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
     3541 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
     3601 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
     3661 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
     3721 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
     3781 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
     3841 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
     3901 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
     3961 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
     4021 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
     4081 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
     4141 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
     4201 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
     4261 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
     4321 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
     4381 ggacgaggac gaaccgaaga aatagcaagg gtttgtttga aaacagtgta aaaaataaac
     4441 catgtttaca tggatttgcc accggtttgc caccggtggt ttttttcggt ggcaaaaatc
     4501 aagaattctt tctaagattt tccatgatat cgaccgtttc atttctcatc ttatctgtta
     4561 catgcgagta agtatccatc gtaattgaaa ttctgctgtg gcccaatcgt tcagagattt
     4621 ctttcatttt tgcgccattt tcgagaagaa gtgtagcatg agtatgtctg agagaatgga
     4681 agttaaaaga gagggagagt gcattcgata tcctcctagt attccatttc actacacttg
     4741 gcgtaactag ctcaccgtct tccttcgtac atactgcatt tgaatcaatg tagagctttc
     4801 catacttcat tcgattttct aattgttgtt tcttatgttt tttcagaatt gctagcaagg
     4861 tttgtccaat aaaaatcgtt cgattggaac tgcttgtctt tggcgtacca tatacccatg
     4921 cgccgtcatt ctttaccatt tgtttctcta cagtaattgt tccatttgaa aaatcgacat
     4981 catcccacgt cagaccacaa acttcgccaa cgcgcattcc cgtataaaat ccaatattca
     5041 aaggaatata gaaaggatgt ccttcaggag taatttctag catatgatca aagtcctcaa
     5101 gagaaatgat ttttagatct tttttagtcg ttggtcgtgc ttcatatttt ggtattttta
     5161 catacagcat aggattttgc ttgattaatc cccaaggata aaccgccata ttcagcgcat
     5221 tcttaaggac agagtgagta atagtcattg ttttcttcga gtaacccttt ttaaattcag
     5281 cattgatgaa attttgtaaa agagcagggg agagatccgt aagttttttc tttcctaaat
     5341 aaccgtttat atgatttttg atggtaaatc ggtagttttc ataggtattg tattttagat
     5401 ttagtttaac gtattcctcc atccaaaaat caaggtattg ttttactcga gtatccgtac
     5461 ctaaaaagta ttgtcctgtt tcgtcaatat ctgataaaac ttttcgtaaa gcagcttcgg
     5521 cctctggtcg ggtgtctccg ccaactttct ccactttttt tcttgagcca tcatcattga
     5581 tatct
//
LOCUS       DAHUWM010000171         5511 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHUWM010000171 DAHUWM010000000
VERSION     DAHUWM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.175"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..152
                     /locus_tag="NK788_002265"
     CDS             <1..152
                     /locus_tag="NK788_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCH6770576.1"
                     /translation="YIINTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKI
                     ENESF"
     gene            328..1071
                     /locus_tag="NK788_002266"
     CDS             328..1071
                     /locus_tag="NK788_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770577.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1064..2644
                     /locus_tag="NK788_002267"
     CDS             1064..2644
                     /locus_tag="NK788_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCH6770578.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2895..3374)
                     /locus_tag="NK788_002268"
     CDS             complement(2895..3374)
                     /locus_tag="NK788_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCH6770579.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3390..4142)
                     /locus_tag="NK788_002269"
     CDS             complement(3390..4142)
                     /locus_tag="NK788_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCH6770580.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4259..4329
                     /locus_tag="NK788_002270"
     tRNA            4259..4329
                     /locus_tag="NK788_002270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4291..4293,aa:Gly,seq:ccc)
     gene            4690..5415
                     /locus_tag="NK788_002271"
     CDS             4690..5415
                     /locus_tag="NK788_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCH6770581.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
BASE COUNT         1790 a          961 c         1050 g         1710 t
ORIGIN      
        1 cttatatcat taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat
       61 ttgcaaatgg agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta
      121 tgcaagaaaa gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt
      181 tagaagagtg ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc
      241 ttttttgtta tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa
      301 gtaataacaa acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac
      361 gggaaaaaac aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga
      421 tttttaggac ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt
      481 gaaccatcat cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt
      541 atcaaacaaa aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct
      601 ggagaatatt gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag
      661 cgtttagcag aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca
      721 agtttttcac atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca
      781 gatatttggg tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg
      841 aaagaaatga tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta
      901 ttggataccg cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt
      961 tatcaggggg cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt
     1021 tatttgaaaa tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt
     1081 attagaatta attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag
     1141 agagaaagga aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc
     1201 agggctgatt tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt
     1261 gccaggtttc tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat
     1321 atcagtcatt tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc
     1381 ttttggacaa gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc
     1441 ttttgttttt ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat
     1501 tttgcttact atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg
     1561 tctggctagt ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa
     1621 aacagtttct agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg
     1681 gatgaatcat caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat
     1741 attattgcca ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg
     1801 gggaatacta gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc
     1861 aaagcttaca gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa
     1921 aaagaaccaa aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc
     1981 taatttgatt atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc
     2041 ttttgcccta ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt
     2101 tttcctggta gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa
     2161 tcttatttct ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa
     2221 ccaatattta aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg
     2281 gatcgcatta ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct
     2341 atttggttct ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg
     2401 gttgctgctg ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt
     2461 gggtttagtc gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata
     2521 cggaatgttg gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt
     2581 tctaatactt tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga
     2641 ataaagaaga ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt
     2701 gttcaaaagt cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt
     2761 ttacaaaact ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc
     2821 aatgtttgtg agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct
     2881 ttgttacact tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc
     2941 tttactttga aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg
     3001 attgctccag caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca
     3061 gtaacgagat ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg
     3121 ggaatattga aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat
     3181 gctactccta aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag
     3241 atccagtaga tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta
     3301 ggtacatttg taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata
     3361 aatatcgtcc ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc
     3421 gcaaaaccga tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca
     3481 taatgtccag ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc
     3541 atcacacatc cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag
     3601 atagcggcta tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa
     3661 aatttcaaac tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt
     3721 aaatcggtca tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta
     3781 acgatttgca accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag
     3841 taaagttctt ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt
     3901 actacttttt ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta
     3961 cgatcaagac caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca
     4021 gaagctaaag caattcgact catcgtatct tctacccgat acatctccgc attgctttcc
     4081 atcatgattt tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc
     4141 atagcatctt ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag
     4201 cgtaaagaat aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc
     4261 gggtgtagtt tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg
     4321 tcacccgctt aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa
     4381 gcaaaaacaa cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt
     4441 tgcgcaccgt ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt
     4501 tttgaataat aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg
     4561 tctctttttt gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt
     4621 ctttctagta tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg
     4681 agtaaagaaa tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt
     4741 gcaggggcat tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca
     4801 actatttttg attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga
     4861 aaacatcaag aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc
     4921 ctttccaaag gaacatggga aagctattca aatattgata aattgaacag agtaggacaa
     4981 gccaatgcta tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt
     5041 gacccgacag gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc
     5101 catttaattg gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg
     5161 acacgttcgt tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc
     5221 aaagaaacaa atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg
     5281 gtagccaggg gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat
     5341 gtctttatct acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca
     5401 aaaacaaaaa attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt
     5461 aaaaacatac attcaaagaa atatagcgta tactttatga aaagagtact t
//
LOCUS       DAHUWM010000172         5479 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHUWM010000172 DAHUWM010000000
VERSION     DAHUWM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.176"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            35..2095
                     /locus_tag="NK788_002272"
     CDS             35..2095
                     /locus_tag="NK788_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCH6770582.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2178..2747
                     /locus_tag="NK788_002273"
     CDS             2178..2747
                     /locus_tag="NK788_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770583.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2922..3728)
                     /locus_tag="NK788_002274"
     CDS             complement(2922..3728)
                     /locus_tag="NK788_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770584.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3866..5380
                     /locus_tag="NK788_002275"
     CDS             3866..5380
                     /locus_tag="NK788_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCH6770585.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1945 a          949 c         1124 g         1461 t
ORIGIN      
        1 atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac gttcaaaaca
       61 actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa aacactacgt
      121 taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata tgcatgtacc
      181 cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg agaccactga
      241 ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc tacttcctgc
      301 attgacttcc tataataaag tcagttatcc tccaacgata aaccactact ggttaattcc
      361 taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta tcttttccga
      421 tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt tgaatcatgc
      481 tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg aaaaagatta
      541 ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt cgatatctga
      601 tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca aagtaatttc
      661 aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt ccttgtccta
      721 ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg ttcgtgctga
      781 tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt acgcccctaa
      841 agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag aagaaatcaa
      901 aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat taacttatat
      961 agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag aagcatctgc
     1021 tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag aacttcaagc
     1081 agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg ctgaattttc
     1141 tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag tcacttctat
     1201 ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg agatccttga
     1261 agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa ttacctttac
     1321 ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg ttcatccaaa
     1381 cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag gtaaacccgt
     1441 accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg gttatttgac
     1501 caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt cactccaaga
     1561 actccatgct cagtcactat tacatgtttt accattaaac aaacagatag atcaaaaaga
     1621 acaaaaaaga cgagaagaaa gcttgtcaga aaaaaaacaa caaatggctc atgcagtttc
     1681 tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat ctagaaaaat
     1741 ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag tttctggatt
     1801 ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat ttgttgctaa
     1861 acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa aacgcttgtc
     1921 aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta gtccgggaac
     1981 acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc gaggtatggc
     2041 aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt tctgatttat
     2101 atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc ataatggaga
     2161 cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc tggatgacaa
     2221 gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa tttcgatttt
     2281 atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg gaatttcaaa
     2341 cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct gaagaacttg
     2401 aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc tgtaccgctt
     2461 ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca gtgaaaccag
     2521 attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa gaatttccta
     2581 ccctccatac ggacggacca atctatcttc acacacagac atggagccat caggcgattg
     2641 ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc acacctaatc
     2701 cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga aatggtgaaa
     2761 taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt acttttttga
     2821 gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg ggcagtgaca
     2881 aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt gattttttta
     2941 ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata gaggtaacca
     3001 aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat agtatccatg
     3061 attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc atctgcattt
     3121 tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt tccgtcttta
     3181 cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc gaattgcagt
     3241 aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat cgaaaaacag
     3301 aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt tttattgagc
     3361 aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc atgtagaaaa
     3421 gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac tgttgaacta
     3481 aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt taatatcttt
     3541 tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat tgtatttccc
     3601 cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg taataaaata
     3661 tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa
     3721 tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac
     3781 caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata
     3841 gtataaggag aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt
     3901 tgcacttctt gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc
     3961 tagtacgaag gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc
     4021 aaaagaaatg aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc
     4081 agcagctatc gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt
     4141 tgccggagga aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca
     4201 aaaagaagaa ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg
     4261 tggaggaacg aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt
     4321 cgattggcta gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag
     4381 tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg
     4441 gttattgaat aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga
     4501 cattaagaaa aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc
     4561 aaaagaggtc accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga
     4621 atttattgaa aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc
     4681 aactggagat ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa
     4741 aatacaaatt catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg
     4801 cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac
     4861 aagggataaa gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt
     4921 tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt
     4981 cacaactgga aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact
     5041 agcaaaaaca gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa
     5101 ccgtgccaca gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc
     5161 accaggaatc cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga
     5221 taaagaaaac cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca
     5281 tggaaacaac cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc
     5341 gggcatacaa gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag
     5401 aggttgtgaa atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag
     5461 tgaagaggtt gtgaaaaag
//
LOCUS       DAHUWM010000173         5376 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHUWM010000173 DAHUWM010000000
VERSION     DAHUWM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.177"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(37..1968)
                     /locus_tag="NK788_002276"
     CDS             complement(37..1968)
                     /locus_tag="NK788_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6770586.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1986..2900)
                     /gene="pfkB"
                     /locus_tag="NK788_002277"
     CDS             complement(1986..2900)
                     /gene="pfkB"
                     /locus_tag="NK788_002277"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCH6770587.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2897..3649)
                     /locus_tag="NK788_002278"
     CDS             complement(2897..3649)
                     /locus_tag="NK788_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCH6770588.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3808..4017)
                     /locus_tag="NK788_002279"
     CDS             complement(3808..4017)
                     /locus_tag="NK788_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770589.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4028..5356)
                     /locus_tag="NK788_002280"
     CDS             complement(4028..5356)
                     /locus_tag="NK788_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770590.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         1511 a         1162 c          939 g         1764 t
ORIGIN      
        1 agaggctggg aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc
       61 ttttctcaat aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa
      121 atacatgacc gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa
      181 tttgattccg aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg
      241 aatggcacga atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag
      301 tcccatcacg atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa
      361 caaacgtgta gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc
      421 catcacgaca cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata
      481 agcggctttg ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc
      541 aagtaaagca gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat
      601 tgctttcata gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac
      661 tggataaaag aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt
      721 acgtaaaaat acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc
      781 tgcaccgcct gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc
      841 accgatactt gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg
      901 tccgatttgg ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc
      961 atgtggcacg ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc
     1021 aataacgaaa ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc
     1081 gatagtacca tccgcatttc cttcttctga actgctttct tttccatcac catggaatac
     1141 tggtgcagta ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact
     1201 gacaggacga ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc
     1261 tgcaacgatt acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc
     1321 ttctgaaccg ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa
     1381 agcatcttct gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac
     1441 gaattttctt tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc
     1501 ttctgcttct ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt
     1561 tgcttctttt aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa
     1621 tgcttgcaaa tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt
     1681 tggctgtccg tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt
     1741 tgcttcttta actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt
     1801 agaagtttgt gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg
     1861 gccggcatca aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg
     1921 caaatccatg atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct
     1981 tgaaactaat caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga
     2041 gctgccaagt catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct
     2101 tcgattggat ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct
     2161 gcacctactg aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc
     2221 gtgaagagca aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct
     2281 agtaattttt tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc
     2341 tcagctaatt cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt
     2401 aggtcatcac ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc
     2461 agttcttgat aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata
     2521 tctccttgtc ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata
     2581 gatgggccta agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt
     2641 gtatcttctt ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa
     2701 ataaattcgc cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt
     2761 ttcaaaatac gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt
     2821 cggttcaaat cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta
     2881 agtgttactg tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat
     2941 taacgaattc taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag
     3001 aggtgaagga caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat
     3061 ttttcatggc taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt
     3121 caagatgggc accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt
     3181 ctatcgcatt agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa
     3241 tggtagcaag ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg
     3301 tgatctgttt tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat
     3361 aaatgacatc ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt
     3421 gaacgttttt gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct
     3481 tagctccgcc atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc
     3541 ttatcgttga ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct
     3601 gctgttcaag caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca
     3661 cctcaataca aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt
     3721 tcataaaaat tataaatcag tcaaaaaaaa ataaaaaacc agtcagaaag actggttttt
     3781 tatttttttg tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt
     3841 catataaaca gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc
     3901 gccaaacatg accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag
     3961 acagacggat aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt
     4021 cttcacctca tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt
     4081 gcacacgcag atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct
     4141 ttattcgttt gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg
     4201 acagggattc tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat
     4261 ctgttacctc tacttctaga tattccctaa tataataaca ataagcttcg attccattat
     4321 ccaaagcaac agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa
     4381 cccaaggaaa atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc
     4441 cttgttggat atcataactt tttgttacaa aaaatgaagc attcttttta ggaacaggtt
     4501 gcatttgtgc attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa
     4561 cgaatggcgg tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct
     4621 ggtccctaaa ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc
     4681 gataaagctg gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga
     4741 tgtcgagaaa aatcggttta gggatgattt gatgataata gcgactataa tacatttccc
     4801 aacacgaagt gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat
     4861 cgactactac ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt
     4921 tctcaaacaa tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag
     4981 ctcgcatata ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca
     5041 aaaattgctt ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat
     5101 cttccaagcg aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc
     5161 ttaccgatgc tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt
     5221 gtgtgaaaga gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc
     5281 ttatttcctg ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt
     5341 tatcttcgcc aatcattttc tcacctcttt caaatc
//
LOCUS       DAHUWM010000174         5359 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHUWM010000174 DAHUWM010000000
VERSION     DAHUWM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.178"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            89..1021
                     /locus_tag="NK788_002281"
     CDS             89..1021
                     /locus_tag="NK788_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770591.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1242..3005
                     /locus_tag="NK788_002282"
     CDS             1242..3005
                     /locus_tag="NK788_002282"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCH6770592.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3089..3547
                     /locus_tag="NK788_002283"
     CDS             3089..3547
                     /locus_tag="NK788_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCH6770593.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3656..4339
                     /locus_tag="NK788_002284"
     CDS             3656..4339
                     /locus_tag="NK788_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCH6770594.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4486..5100
                     /locus_tag="NK788_002285"
     CDS             4486..5100
                     /locus_tag="NK788_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCH6770595.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5116..5322
                     /locus_tag="NK788_002286"
     CDS             5116..5322
                     /locus_tag="NK788_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770596.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1911 a          842 c         1103 g         1503 t
ORIGIN      
        1 gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg aaaataaaaa
       61 tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc atttttttaa
      121 aagaagatat tcggaagatg aaaagtcctt tatcatacga ggaatagaga tcgcaatcgg
      181 aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag aaacgaagcc
      241 gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag acttacaaga
      301 agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca atgagaacat
      361 ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca atgacctatc
      421 actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg cagaaaattt
      481 tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg gacaagtatt
      541 tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt
      601 agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt
      661 cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg ggagacagga
      721 aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc gaacccaatt
      781 tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc gttcattaga
      841 agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag
      901 ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat taaaaggtgc
      961 aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac atatttcttg
     1021 atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag aaggttttta
     1081 tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg aaattttttt
     1141 aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg taacaaatct
     1201 attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca gtattacatg
     1261 tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac
     1321 agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg
     1381 ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta tttgattcac
     1441 caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag cttttaactg
     1501 gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat tccaaccaac
     1561 atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc agcttagcaa
     1621 caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta gcatttatcg
     1681 gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt ggtgttttac
     1741 aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag aatcatggca
     1801 gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc aataacctat
     1861 ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg gaaacggtga
     1921 tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac gtccatacgg
     1981 aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat tggcatgtac
     2041 catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca tttaatatga
     2101 tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt gccgcagtca
     2161 atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat ccagatcatt
     2221 attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct ttggcttctc
     2281 agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca tatgatcaat
     2341 tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt ggcggaacga
     2401 tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta tcaagtatcc
     2461 cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg ctagattggg
     2521 cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt gtagaacatg
     2581 gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat aatggaggaa
     2641 aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt
     2701 ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca gaaaatgacg
     2761 tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta gaagatggta
     2821 gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct tcaatgaacg
     2881 tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg cagctttatg
     2941 aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc
     3001 aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg atggtttttt
     3061 cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa
     3121 gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt catttgggga
     3181 tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa agactggtac
     3241 atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt
     3301 agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata taaatgtgac
     3361 catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg
     3421 gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt ataaagaagc
     3481 gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg aagtaatttt
     3541 cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag
     3601 ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag atattgtgga
     3661 tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc
     3721 gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag gcggagcagt
     3781 tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg ttgatttacc
     3841 ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta ctgcgacaga
     3901 aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg atgcaacttc
     3961 tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc acgaacacgg
     4021 tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg
     4081 ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa agacgcagac
     4141 acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag taattatgga
     4201 aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg ctttttcagt
     4261 cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg tcgaagccgc
     4321 aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt
     4381 gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt cattttgtct
     4441 tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt tgaacggagt
     4501 ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt catccctaga
     4561 ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc acccttcagt
     4621 tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca gagaaaacaa
     4681 gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt tatccatatc
     4741 cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc taatgaggaa
     4801 agcgaactgt acctgactga cttgacttta actgattcag taatcatttc agtaccagca
     4861 ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga gatacggaat
     4921 aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca tttaaatgaa
     4981 acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc tgtaagccgt
     5041 ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg tcccgaataa
     5101 aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc aaaagaaatg
     5161 aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga agttccgcca
     5221 ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat agaaatgcca
     5281 gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat tccgaaaagt
     5341 gaaaaaacat ataaaaaaa
//
LOCUS       DAHUWM010000175         5308 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHUWM010000175 DAHUWM010000000
VERSION     DAHUWM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.179"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(19..240)
                     /locus_tag="NK788_002287"
     CDS             complement(19..240)
                     /locus_tag="NK788_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770597.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(237..527)
                     /locus_tag="NK788_002288"
     CDS             complement(237..527)
                     /locus_tag="NK788_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770598.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(524..1708)
                     /locus_tag="NK788_002289"
     CDS             complement(524..1708)
                     /locus_tag="NK788_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCH6770599.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1890..3293)
                     /locus_tag="NK788_002290"
     CDS             complement(1890..3293)
                     /locus_tag="NK788_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCH6770600.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3315..4088)
                     /locus_tag="NK788_002291"
     CDS             complement(3315..4088)
                     /locus_tag="NK788_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770601.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4098..4475)
                     /locus_tag="NK788_002292"
     CDS             complement(4098..4475)
                     /locus_tag="NK788_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6770602.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4496..4810)
                     /locus_tag="NK788_002293"
     CDS             complement(4496..4810)
                     /locus_tag="NK788_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCH6770603.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5010..>5308)
                     /locus_tag="NK788_002294"
     CDS             complement(5010..>5308)
                     /locus_tag="NK788_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HCH6770604.1"
                     /translation="KMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNV
                     SDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1555 a         1057 c          915 g         1781 t
ORIGIN      
        1 tatcatgaaa aatatctgtt aagcaccaat gattcggtta aataagttta ataacacatc
       61 tttcacgcca gctgcattga acacaagtcc gacggcgatt agagcaataa tcaagaagcc
      121 aatgagtttt gaaaattctc gtttaaaacc taaaaacagt ccaatgacaa caatcgctaa
      181 caataccaat gactgtgcat tatccaagaa ccagttgtac aaattctgcc caaaattcat
      241 gtgattgtcc tcctttcctt aagatatttt tctcgataat agatatagct ttttacacgc
      301 cttaataatt gaaaaggggt taaatgctct ccatagacgg ttactcgatt ggtataatta
      361 ttataaaatc tcaccttgta aaaatcatag gtatagatgg tatcaccagc ttgccgtttt
      421 ccctcaataa tgagagacgt atatttccct gtattttgta tatacgcttg aaactcaatg
      481 ccacactgaa accctaactt ctttaattct tttgttaaaa ggctcatgta atcaaatcct
      541 ccatggctag atgttgttgt tcaattaatt tctcatgttt ttctgtcagt ttggcttcct
      601 gaatcatggt ggagataatg gttgtaccgt tgagaacatc taaaatgctt gcgattttta
      661 atgtaggggc gacttgatga tgtaaccagt tcaatgttcg ttcaaaagaa taaggctctg
      721 gctgtgtggt taggcgtaat gctccacgat ttttaccgat aaaccattcc caacgttcat
      781 tagttttcca atcactccga cgtttagtgt cgtctttatc tgcaaagcgg atatagcgat
      841 tgataatttc aaaggctgtt ttttccgcct gttgatgtgc caataaatct ttcatggctt
      901 gggtggctcg gtcatttttt aaccgaattt caaaccgatt ctttacttct gcgtcttcta
      961 agggaacatc atttttcacg tattgctcat aatctttctc atatatacag aaatagactt
     1021 cactttttaa tgagccaata taaagtgtgt ttcccatgta tggtttttca tcacggtgaa
     1081 ccaattcacc actgcgataa tttttaaagc ttcgaaagac ggaaatgcat tcttcctgtt
     1141 tacatttctt tgccaaatct ggaatattca aaatccctgt tttatcgttg atagctaaat
     1201 ccaagcgttt gaaaatacct ttatgttcca tgcaatcttg aaagaaatca taccagctcc
     1261 gatgttgagc caatagaaag ttttcaaatt gccgacagcc ttttcctttc agttctaata
     1321 aaacaccttt acttacatca tgagagacta ggacaacaat atcaccgaaa cgatagtgtt
     1381 cgggataaga ataatagcca aagtcttcgt gtagcatata atccatattc aaacgtaaaa
     1441 cctcaccgat aatttgagcc acatcattgg tagggaatcg aatccgtaca tagtcgaata
     1501 acatctccaa tgggttatcg ggattgaagc gttccaaagc gttcagtaaa tcttctttca
     1561 cctgttgagt agggactgcc ttgcctgttt caatatccga taggtaaggt ctggtaatat
     1621 gagaagcaac cgccagttta ttttgagaaa cgccataagc cagtcgcttt tctttcaatt
     1681 ccttaatcca aattttttcg ttttccattc gcaaacctcc aatctaaaaa agatgtaaac
     1741 tatgaccctt gagtttacac ctttcatatt ttccgcaatc ctttgtacca tcaacattgg
     1801 ttgatgagaa ttgcctgttt tcagttgtat ttgtgccccc ctgttagata gagggggcta
     1861 aaggattggc gtggctaacg ccacaccaat caaagcaagt ctgaacctgt acctttcact
     1921 tcgtgcggta caggctcaga ttgctttttt tgaatgactt ttcctatttc tcctaagaaa
     1981 tcgtaccttt ttggtacaag gggtgtataa aactcactaa tcacactttt tcctgtatcc
     2041 acgtaacctc ttcccttgat gggttttaag aaaaattgtt tttgtgtatc gcttccgaac
     2101 atcataccaa agcccatttc agacatacga cccaaagcca cacggaagtt aaagttgtcc
     2161 cgaatcccat cactaaaata ttttgcgtct ggacgttgac aggcaagaat taaaaagaag
     2221 cctgcttgtc gtcctaacat tacaatttgt ttgagtttat gaataacggt ggtactttca
     2281 cgacctagca tatccatcat tgccacatat tcatcaaaaa taagaaaatt ggcagacaaa
     2341 ccaagataag cataattttc tcctgtttta tagttagggt gttgtttcat ggtttcactg
     2401 cgatcaatca tatcttcgta aaattgattg atacatgcca acatatcttc tttacggtaa
     2461 tagacattat tcatgaccgc ccctaagtct gccaaatcag catttttagg gtcaagaata
     2521 taaagctttg catttgtatg caggagagat tcaatcaatg tgagaataaa ataggatttt
     2581 ccactaccag tgcccccagc aattaacatg tgagggagag aatcgaactc ccaatagacc
     2641 gattccatca atttgacacg tccatcttga gctaccacgt cttcaatgcc aattcgattg
     2701 ccaatagtat cataaagtag gacatactct acataagaat ctttcaacgt tttttccact
     2761 aattcacaat acaatcctgt ttctagtttg ttttctaggt gcaaaagctg atcttgatat
     2821 ttccctaagg tgatttctac atgaatatgg attagtccat ctttcattcg ataatatatt
     2881 ttaggaaaat ggcggattcg ttgtttggtt ttcttgctgg gtaaatcttt aaagaatccc
     2941 tctgattgag tttcttcggc ttcataccaa tgattttcta aaatcatacg agctaacttt
     3001 tgacggtgct ctaactgttt tatagaatct atacgatagt gataataaag ccagctgata
     3061 agtccgcata caaaaagagc agtgagaata ctgctccaaa agtatggcgt atgaagttga
     3121 tagaaaaatg tatctaactg aactgcttgc cagttcaccg attgtaacgc tctccaatga
     3181 aaagggagta ggacgactag aaaaactacc agcaatgaaa ttagtacaaa atgatagacc
     3241 aagtgcttat cgttggcttt gattcgctct cctctgtcat gaagcaatgg cataaaaatc
     3301 ctcctttcca cctatcaaaa taattgtgta ttgtagatac gagcctgttt atcaaaagtt
     3361 ggttgagaaa tggcttcata tttctgataa gcgattcgct tttcaaagcg taaatttttt
     3421 tctttcggat aataatgacg tcgatagaga gtgtgtccta acagtttttt cgcttctttt
     3481 gtcaacggat agagatagcg aaacatgcgt ccattgattt tctcaatccc tttgtattcg
     3541 caaaatgcct gggaaagcca atgctttttc tctacctgtt caaagcgagc attttcctct
     3601 aataaaaggc gagcacttcg agggtgtatc ttttcccatg actgcttatc acgatagaca
     3661 cttgtcttga aatacccgca gtaaaagaag ttggacgctt gatagacata gccacatttt
     3721 ccctcaattc catctgccag tgtatataaa aaatgacagt ccgtatgttc tttcaaccac
     3781 ttaattagag ctgaaagagc ttgactgcca aaataattgg tttgattcat ttctggaaga
     3841 aaacacatct ttccaatttc taaataatct gtggtttgaa gggaactgtc gggaaagagt
     3901 ttgcgtatgg tttgtagggg ttgcgtaccc cagcctaatt ccacaacgcc taataatttt
     3961 tcttctgaaa aaatccctag aaagtattta cacaatctag ggagtatctt tgaataatgg
     4021 tattgccgaa taaaagaaat ggcttcatcc ttcggaattt ctttcaaaat caatggtgtg
     4081 tttgtcataa gcaacaccta tttttcactt tcggacgctt cttttttata tttctgatga
     4141 ggttttgtct gtccttttaa tacaagatcg tctgctttca cgtaccaatc aacgtctgcc
     4201 cctcgaaaag tagccgtcgc tacggtatct gccacaggat tgattaattc cacttctgcg
     4261 ttgtagtcaa attctttcaa aggaacagaa gcggggatac tgacttgtat catgcgtcct
     4321 tgtccttttg attttaagtc ataggtacgt tctttaattt cttcggaaac cgtaccatct
     4381 tcattctgga tacgaacctc acgacgcaag gcggaaaatt tcaatgtgcc aaatgtctgg
     4441 tctttttcaa tcacaatacc ttctgctaat ctcatatttt ttcacctttc ctttcttatg
     4501 ctttcaccat atcgtctgcg tgcataatgt aattggtaaa tcctcgattg ccaattttat
     4561 aaccttctgc ggtaatatgt gggttgatga gtttgacttt ttcgtcggat tcaaaatgtt
     4621 tctcgccagc ttcggaggga agaatgacaa caatatcatc tgctcgttga atcgttgagt
     4681 ataaattgta actacgagag agaatggttg gtctgccatt tattcgacgt tgttcaatgt
     4741 tcccttcacc agcatattct aattgtccaa atgttttttc catattggga atgacatatt
     4801 taagttccat atttttacct atcctttctg tttttatgtt tgaatgaatc ttatttgtgt
     4861 gatggtttta aaggggggag agcgaccttt tgattcttga ttttttattt tcataagttc
     4921 acttcctttc aaaattgggt aaaaaaatag acgctccgta ctgagcgtcc gattcctact
     4981 atatttaaaa caacgactac ctaggtattt taaatattat aatcaaattc tattttatct
     5041 aataattgag tgggataaag caagtctcct ttactcgtaa aataacccac ctctgagaaa
     5101 agaaataaat tattttttgc tcttgaacaa attacgtata gtagttttct cgctgaacta
     5161 attcctttat cagaaacatt aaaaatttca ttccaatgag gaattttccc gtttagaaga
     5221 ttaatagcga taacagtatc atactcattt cctttaactt tatgtgctgt agtaagtgtg
     5281 ataccatctc gagatttaaa catcttct
//
LOCUS       DAHUWM010000176         5275 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHUWM010000176 DAHUWM010000000
VERSION     DAHUWM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.180"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1363
                     /locus_tag="NK788_002295"
     CDS             <1..1363
                     /locus_tag="NK788_002295"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCH6770605.1"
                     /translation="DLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQL
                     ASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEF
                     LISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRN
                     YLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFD
                     AVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVL
                     PHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLF
                     IDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRY
                     VDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFK
                     HEQ"
     gene            1353..2570
                     /locus_tag="NK788_002296"
     CDS             1353..2570
                     /locus_tag="NK788_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCH6770606.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2583..>5275
                     /locus_tag="NK788_002297"
     CDS             2583..>5275
                     /locus_tag="NK788_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCH6770607.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDS"
BASE COUNT         1843 a          906 c         1099 g         1427 t
ORIGIN      
        1 agatctgatt gccacaatcg ttgatatttt gggatatgca attgcaccag agtatctttt
       61 caacgtctta gcggatcaag caaaacaagc aacgttccaa ttgaacgatc taaacaaagc
      121 ctttgtgcaa ttggcatcta cctacaatca atttaatggg ttatttgatg atgtggatct
      181 ccaatcaaaa aaactaggaa cagatgagca acaacgaaat gtgacgatta ccgaagtcat
      241 caagaaattg aatgatgtag atgttttaga acacgatggg gacgtgatcg gggatgcgta
      301 tgagtttctg atcagtcaat ttgcttcaga agcagggaag aaagccggag aattttacac
      361 acctcatatg gtttctgata tgatggcgca aatcgtaacg ctcgatcaaa aggaacgacg
      421 attctttagt gtttttgatc caacgatggg ttcaggttca ttgatgttaa acgtacgtaa
      481 ctacttaaca cacccagata acgttaaata tcatgggcaa gaactaaata caacgacata
      541 caatctagca aaaatgaact tgattttaca tggtgtcgac gccgaagaaa tgaatctccg
      601 caatggggat acgttgaata aggactggcc aacagatgaa ccttacacat ttgatgcagt
      661 agtaatgaac ccaccttatt cggctaattg gtcagcagat acgactttct tagatgattc
      721 tcggtttaat cgttatggga aattagcgcc aaaatccaaa gcagattttg cgtttcttct
      781 tcatgggttc tatcatttga aggaaacagg taccatggcc atcgtcttac cacacggagt
      841 tttattccgt ggcgctgcag aaggggtcat ccgtcaaaaa ttactagaag atggcagtat
      901 ttatgctgtg atcggtatgc ctgcgaactt gttctttggg acatcgattc caacgacggt
      961 gattgtgttg aagaagaatc gtcaaactcg agatgtatta ttcattgatg ctagtcgtga
     1021 atttgttaaa gggaaaaatc aaaataaact atcggaagaa aacattcaaa aaatcctcga
     1081 aacctacgct gaaagaaaag atgtcgagaa atacgctcat ttggcaacct ttgatgaaat
     1141 caaagaaaat gactataact taaatatccc acgctatgta gatactttcg aagaagaaga
     1201 accaattgat atggttcatg tcggaaatga catcaagaag atacgacaag aacagcaagt
     1261 gctagaaaaa aaacttttag aagctatctc ttccctgcaa acaacacctg aaaatgaagc
     1321 gtggttacaa ggtgcgctgg aggtgttcaa acatgagcaa tgatacacaa ccagaaatcc
     1381 gtttcccagg atttacagat gattgggaac agaggaagtt gggggacttc gttgtagatt
     1441 atgttgaaaa gacaagtgta caaaatcaat ttcctatgct aacttcatct caacaaaaag
     1501 ggatagtttt gcaagaagac tattttgcaa atcggcaagt aactacagaa aataatattg
     1561 gttactttgt actacctaga ggatatttca cgttccgtag tcgaagtgat aatgatgtgt
     1621 ttgtgtttaa ccgtaatgac attattgata gagggattat aagctatttt tatcctgtat
     1681 ttactttaaa atctgctgat tccgacttct tcttaagacg tattaacaat ggaatccaac
     1741 gacaattatc tattcaagca gagggaacag gtcaacatgt tctgtctcta aaaaagttta
     1801 aaaatatagt agcaatgttt cctagtgagg aagaacaaca aaaaatcggc accttcttca
     1861 aacaacttga cgacacgatc gctcttcatc agcgtaagtt agatttgctg aaggaaacga
     1921 aaaaaggatt cttacaaaaa atgttccctg aaaacggagc aaaagtgcca gaaatccggt
     1981 ttcctggttt tacggaagat tgggaagagc gtaagttggg agaattaggt aaaactcaat
     2041 cgggaattgg atttcctgat gccgagcaag gtggttctga aggtactcca ttttttaaag
     2101 tttccgacat gaataacatt ggtaatgagt atgaaatgag aaatgcaaat cattatgtat
     2161 ctaatgagca aattgagcgt aaaaaatgga agccaataaa agatgtgcca gcgattatat
     2221 ttgctaaagt tggcgcagca ataatgctta atagaaaacg tttggtcaca agccctttct
     2281 taatagataa caataccatg gcttatttat ttaataatac ttgggatatc tattttggta
     2341 aaatattgtt tgaaactata aatttgccaa gatactccca agtaggggca ttgccttctt
     2401 ataacagttc tgatattgag aatatcagtg ttaaggttcc tgtaaaggat gaacaacaaa
     2461 aaatcggtac tttcttcaaa cagctagatg atacgatcgc tcttcatcaa cgtaagttag
     2521 atttactaaa agaaacgaaa aaaagcttct tacaaaagat gtttgtctag gagggaagaa
     2581 gaatgaaaat tagtcaccgt gaggaagcag aagtcgaaga acagttgatt cgtgtacttg
     2641 gagaagggca taatcagtgg acgtatcgtc cggatctaaa atcagaagaa gacctttggg
     2701 tcaatttacg ccaaaaaatt atctcaaata atcaagcaga actgaatgat tttcctttga
     2761 ccgataaaga atttgaaacg atcaaaacgg aattactgct gcgaacgaaa accccatttg
     2821 atgctgcgaa atggttaaaa ggtgaaaatg ggatggctcg gatcacgatt gaacgggaag
     2881 attcgcaatt gggttctgtc tcacttatcc tttattccaa tcaagacatt ggtggaggaa
     2941 tctctactta tgaagtggtc catcaaattg caaaacgtgg aagtaacata gaagcacggg
     3001 atcgacgttt tgacgtcacc ttattaatca atggattacc gattgtccaa atcgagctga
     3061 aacaagtaac tgcaaaagat ggtgtttatc aggcatttaa ccaaatcaaa aaatatgcag
     3121 aagaaggtat gtttcggaac aatatctttt cgaccttaca gttgtttgtg gtttccaatg
     3181 aacaaaccac tcggtatttt gcaaatgcct taccgaaaga cttgcaccct aagtttttat
     3241 ttagttggcg aacgaaagac aatgaaaaag tagaaaactt atatgagttt tgtaaacaag
     3301 tgttaaatat cccggatgct catcgtttga ttgcggatta tacaatcgtc agtgaagatc
     3361 aagacaataa aaccctcatg gtgttacatc cttatcaagt acatgcgatt caagccttgt
     3421 ttattgccgc aaataaacat caatcagggt atgtgtggca tgcgactggt tcaggaaaga
     3481 cgttgactag ttttgtatct actaagcttt tagcacgaaa atcggggatc gatcgaacga
     3541 tcatgttagt ggaccgcaaa gatttagata atcaaaccac cacagagttt acaaaatttg
     3601 cttcggaatt taatactggg atttcttccg ggaacgccaa agcgaatagc ttgatcgttg
     3661 gaacgggaag tgccaaagaa ttaagtgaaa ccctgttggc agatgccaat gcaaatgttg
     3721 taatcattac aacccgtcaa aaactagatg cggcgttgaa atatgcaaag aaacaagaag
     3781 agaaaaaagg aaccaatcgc ttccaaaaat taatgggcca acatatcgtg tttgtagtag
     3841 atgagtgtca tcgagcgtta agtgctgaaa atatggaaga aatcaaaaaa atgtttccga
     3901 aatccacttg gtttggcttt acaggtacac cgatttttcc tgagaatcgg aaacaagcca
     3961 aagggcaatt ggcacgtaca acccatgatc agtatggaga ggtcttgcat acctatacca
     4021 ttaagaatgc gctagaagat ggctctgtct taggatttca agtagagcat gaaaacaccg
     4081 tggagccgac ttcactagag aataaaatct atcggaaatt aaaagaagta gaaacatatg
     4141 cagagtattc atcagaacag atcaatcgta tgattgatca aatggaacct gtcaaaaaag
     4201 aaagttattt ggacccagct gtttttgaag cagatgaaca tatccaaaaa gtcattcata
     4261 aaatgttccg tccagataat gcctatacaa aatttgattt tcgaaatggc aggccaacga
     4321 aatctgcaat cttaaccacg agttccattg acatggccaa aaagtattat cgggccatca
     4381 aagagatgac gaaagagcct gattggttaa caaaagagtt tcccaatcag ctcattcgtg
     4441 aaggacgaac gatggaggat cctgattttc cacgaatcgc tattacctat tcattagaag
     4501 aaaatagccg agattcaagt gcccaacaag aagaaatgca aaaaattatt gaggaatata
     4561 atcagtatta tgatacttcc tggtcattag cagacattga gcgttataac ggagacatca
     4621 ataatcgttt ggcacgaaaa agagcggaat tcaaacaatt tgggaaacaa atcgatttag
     4681 cgattgtagt ggatcgtcta ttaacaggtt ttgacgcccc tacgattcaa accctatttg
     4741 tggatcgtaa tctagagtat gcaggattga tccaagcttt ttcacgtacg aatcgtacgt
     4801 atcctgataa gacaaaagga ttgattgtga cgtttcgtaa gccagcgaca atggagaaga
     4861 atgtcgagga tgcgacaaaa ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc
     4921 caagttatca ggaatccaaa aaaagattca aacaagcata tggtaagtta aaagaggttt
     4981 catccatgga atccattgat gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag
     5041 catttcaaga attgaatcgt gcttatgaat cgttagtgac ttatgacgat tacaatgatg
     5101 acatggtgaa atctccaact ttgaacaatc aaattcaact tttggaagaa caaattggcg
     5161 tgtacgagac cgtgaagggt tcattaatag aggaagaacc agaaaaagaa ggagaagatt
     5221 tttcaacgat cgaattttat agtgaaaata ccactaagtt gtatgatatt gattc
//
LOCUS       DAHUWM010000177         5249 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHUWM010000177 DAHUWM010000000
VERSION     DAHUWM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.181"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..1665)
                     /locus_tag="NK788_002298"
     CDS             complement(<1..1665)
                     /locus_tag="NK788_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCH6770608.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1707..2765)
                     /locus_tag="NK788_002299"
     CDS             complement(1707..2765)
                     /locus_tag="NK788_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCH6770609.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2884..3339)
                     /locus_tag="NK788_002300"
     CDS             complement(2884..3339)
                     /locus_tag="NK788_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770610.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3923..4726)
                     /locus_tag="NK788_002301"
     CDS             complement(3923..4726)
                     /locus_tag="NK788_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCH6770611.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1550 a         1026 c          727 g         1946 t
ORIGIN      
        1 attttgacca tatactcttt ctttcctagg tggaagacgt aaatctatat ttaaatgttt
       61 tcgtactaat tcagcactct tcaacatttc attataagca atttccagaa gagtttgttc
      121 ggataaagcc caaaatggaa ctattcgtcc aaatcggtta ataacttcag gctttagacc
      181 ttcaccaatc aaggcttgtt tgatccctga tgcgactttt cggtattctc tttttagttc
      241 agtgctgtat tcattagaat tttccttgtt tgtaacacga gatagtgagc tatatcggtt
      301 taacgaatcg aacatctccg cacctgcatt tgttgtcgca aataaaattg tatttgacaa
      361 tgaaacggtg tcgtgaactt ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc
      421 ttcatctaaa attgataaaa aggcatcgag tgtttctaca ccagctttct catattcatc
      481 acacacaata atcgcagaag gatcatgcct tacatgagta cctataagtc gtctaaaacg
      541 cattgcatca tctttggttt tgtagtccgg catattaact aagagaactt tattttcatc
      601 gccataaagt cctcttgcca cggctttagc cattgcagtt ttaccgaccc ctgttgatcc
      661 tgtaaacaga gcagttgctt ttggttgctt tcgaccttta aaacgatcaa atccaagctt
      721 catacgttta aacaacattg ttatagtata aactgctaac ggttgtcctt ttatggactt
      781 taattcctct tgaagttttt ccatatcaaa atcagaatct agctgaatat tatacttacc
      841 aaatatttgc aatagacttt ctcgattata agcatttccg tctactcgat cttttgcaat
      901 caaatcctcc attacagcaa ttgatttagc cggttcggac aaatcctcac gataaagttt
      961 gtttgcttgt acgatttctt gaagtaattc atcaggaatc ctttgcgata atctaaaaga
     1021 agcctgagga tcatctttaa cagaggatgt atttagccat tgtctcaaaa tatcgacgac
     1081 aacatcgctt tttagttcat tcaattctaa cggttgtagc cgtctcccta aggcatcatc
     1141 tcttttgata tagttttcat attcttttct tgtagtagcg gtaatcattt gaagtgattc
     1201 atttgctaac ggttctttta gcgaatcacc cccaatttta ttgccactcc caaaagagct
     1261 aacaacttta tgaaactcgt caatgaagag gacaaattca ttatctttgt cctcagcttt
     1321 taaaattcgc tcatattctt tcaatcttgg aattaatgtt tctaaacgtg tttggaggac
     1381 atcatctccc ccaacacgca attgtccgat tgataattta tatacttgta ctttctttcc
     1441 atcttgtcgc tgtctttcag cccattcact tgcaagtgct gttttacctg ttccggcttc
     1501 acctaacaag atagcttgcg gtgtttccgg tctattcaag atcagtgaca actcatggag
     1561 ttcgtcatca cgtcccacta acttatgttt tggtggtttc attggtgtga gagcatgatt
     1621 aaattcggtt tgaatattct taaaaaactc ggtactattt tccatctgtt tgtgttcact
     1681 tcctctaaaa taagaataat ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc
     1741 ttcatgtctc caaaagaatc tttgttaaag tcagcatttt tgtattgatc aatcaatttt
     1801 tgtgtatcat tgattgcttt aatttcggtc tccattcgac tgtctagttg acgaatactt
     1861 tcttgcgaat ctttatcgtc aggattttgt tgtaaacgtt ccagatactc agcttttgtg
     1921 gcttcaattt tcttgatgtt agtatttgaa gcatttaact gatcttctag cttatttaca
     1981 acattattct tacttgtaac tgctacaact tttgaataat caatatcgcc atctttggta
     2041 agaaagcttc cttgatagac attagttgaa tctgaatatg gatttattct gaaattgata
     2101 ttatcaggag tattaacagg ttttgctttt ttcccaaaag aaattactcc ttcattatta
     2161 acatcattca tagaatattg agaaagtgct tttgtaactg aatctgcttc attcacattg
     2221 actttggtat catcgtcagc tgtatcatca ccagaaatta gtgatacttg gtttgcaata
     2281 aaaacttgat aagctctttc ctctaattta ccttttagga ataagaaagc atccccatca
     2341 gtacttaaca tcccatagct catttcaagc ttcttaggac gatctttttc ttttgttgcc
     2401 atatataagt tatagttttt tccttgcgta gataacaact ttctagcgtt ttgggagtaa
     2461 ccaagtttta ctactgttac atctctattt tgatctgtcc acacatctga aatagtgatt
     2521 tctgctcctg atcgagcaaa ctccaatgaa gaccctatag gtgttgtatt gttcacgaga
     2581 taataattat agtttcctaa ttgataaatg tagctaccca agcctgttaa taaaaaaagc
     2641 actcctagaa aagttgcaag cataatgcgt aatccgtgac gtgttctctt atccttatca
     2701 ccaagcaacc ctccaataat actgtattga tctttggtag atgttgaatt gacgtttttt
     2761 tccatcagtt tgtctgcccc ctttcataaa agagcaaaca atcagacgtt ttttcggtaa
     2821 ttctaggttc gggattttga atcatttcgg tatattcttc cccattaata tttaacacaa
     2881 aaattatctt cctttcttcg ttcctttctt ttgctcttta ttttccaaat aatcaatgat
     2941 ttttgctatt gtaccaagat agtaattccc ctcagctttg ttttttcgga ttttgctaac
     3001 cattggagta ggtacaatct cttcatctct caattcactg aaagattttt tacctagata
     3061 ttttccaata acagatttta aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc
     3121 ttttagttca tggttcatat tgtcaacgac ttttgcggtt tcaataattg ttttagttct
     3181 cagcttaata atttcatctg gaattttcaa tcctcgtaat ttcatcgttg tactaactgg
     3241 taacccatgc tttactgcat cagcaatact aaaacttttt agatcatcta gtaagctctc
     3301 catatagtta acaaattcat gccaattaat agcattcatt gtctcacccc ttcatttttt
     3361 taaaatgagg ttcgggcgta acacatttat gttattataa tatcataata acataaataa
     3421 atcaatcagc aaatttagat atcccttaaa gtatcagcaa ttattcaaca agatatttat
     3481 tttagctatt tcagtcatca taaaagacta attagcaata aaataactaa tattatccat
     3541 ttatcagacg aaaagaaaaa acttatctaa ttactctata ttaactttta acaaatagtt
     3601 gacttgtcca aaaatgttac cttcaatctc acaaagatat taagatgttt cctaccaaaa
     3661 ctgatatcac cggacttttt attctgtgtt gtccaaaagt gttaccttta gtcgatcaac
     3721 aaattaaaaa aaattgtttt tttcttgact tgtccaaaag tgttacccat agttgttaaa
     3781 atttgtatag attagccatt ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa
     3841 acttcttgca tttattgtcc aaaagtgtta cccatacttt tttaaagaag caggttgtat
     3901 taaaattaca acctgcttct tctcaatctt cttctaacca attatgcatt gaaatggttg
     3961 gcaagtttga ttctggctca ctaaaagtga atctgagccg atcaactctt cttcctttct
     4021 ttgcatagat tttttcataa tcaaaagttt caaatacctg tattccgttt tcatcaggag
     4081 ctaaaaattc ttctttcgcc tgctctagta ttcttttgtc aatatttgtc attctataac
     4141 ttgaaggtat acttaatgct cttctaaaat cttcaacttt gactacccaa taaccatttc
     4201 gccactcttt atttctgtat tgcatgagat gacgatacaa ttcttttgtg tagctacttc
     4261 ttagttttgt aaaattatca agttcaaaac gagtaaagtt tttgccaatt gagtttaaga
     4321 taaattcaaa ttcctcattt gccttaattg taactgtttc cttctgttta tctatccgga
     4381 atttttgaaa aagccacagc tgttcataga cgtctgaatc ttcaaaacgg taggttaaag
     4441 tacctagttt gtcactcaaa gcttttaaca cagaataaaa actgtccttt tctcttctgt
     4501 cgtatttgac caaataacga aagaggttga aatcaaaagt tacttctgct gttccttgac
     4561 gtttcatttt tgataaaata gcccaaaaca catccatttc aacaggctta aatcctcgca
     4621 aagctgttgt attcagacca ttgttataac gcacaattgc atttgtttgt tctgatacta
     4681 aatctacatc actatcaata atcttttcgg taacttcttg agacattgat tactctccaa
     4741 tcttcttttt gcgctccttt aatcatgtta atctggtact aattaaagga aacaattttt
     4801 tctgcttgtt acctaaatta aatcacaact aaaggtaaca gtcaacaaag atttgttact
     4861 aatctaattt tctatttttt cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc
     4921 tagtgtgata cctcaaatgt ccacttttgt tacctttgct gtccaaaagt gttaccctta
     4981 ctgtccactt ttgttacctt tactgtccaa aagtgttacc tttatacccc tttaatactt
     5041 actctcccaa gagattaagc ctcccctaac agatatatta acagatattt aacagatata
     5101 ttaacagata tttaataaag gaggattact cctctcttat ttcaaaatct taaaaataaa
     5161 aaatcttaaa aaaaactaac actatctagg agtttatttt gcttgtgtaa ctttgaataa
     5221 ttgaaaaaaa ggaaaaggag gaagcttca
//
LOCUS       DAHUWM010000178         5232 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHUWM010000178 DAHUWM010000000
VERSION     DAHUWM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.182"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            170..703
                     /locus_tag="NK788_002302"
     CDS             170..703
                     /locus_tag="NK788_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770612.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            715..918
                     /locus_tag="NK788_002303"
     CDS             715..918
                     /locus_tag="NK788_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770613.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            1240..1620
                     /locus_tag="NK788_002304"
     CDS             1240..1620
                     /locus_tag="NK788_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCH6770614.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1602..2516
                     /locus_tag="NK788_002305"
     CDS             1602..2516
                     /locus_tag="NK788_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCH6770615.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            2761..3450
                     /locus_tag="NK788_002306"
     CDS             2761..3450
                     /locus_tag="NK788_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770616.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3571..3657)
                     /locus_tag="NK788_002307"
     CDS             complement(3571..3657)
                     /locus_tag="NK788_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCH6770617.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            4333..>5232
                     /locus_tag="NK788_002308"
     CDS             4333..>5232
                     /locus_tag="NK788_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCH6770618.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALS"
BASE COUNT         1882 a          918 c          989 g         1443 t
ORIGIN      
        1 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
       61 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
      121 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
      181 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
      241 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
      301 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
      361 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
      421 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
      481 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
      541 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
      601 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
      661 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
      721 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
      781 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
      841 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
      901 aagtctaaaa aaaaataaat cgatacattt tagaagaata gggttgtttg aatattacaa
      961 agcaacaatt tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc
     1021 tagccttttt ttgtattgtg gagcgtggat tttgaggggg attcaagggg gagcttgcgc
     1081 tcctttgaca agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc
     1141 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg
     1201 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca acatcaaaat
     1261 ttggctagcg atcattctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     1321 cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt
     1381 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     1441 ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa
     1501 attgccaagt ggtgcaatac acaccaacac gacgttgctc ctgatgatgc tcaacgttta
     1561 tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa
     1621 gtcgaaccac ttcatgtagc cgagcaatca attacgcaga accacgagca accgtcaaaa
     1681 caggaataaa ctgtgacatt gattatgcca aaagtgaaat gaaacaaata cgcacgcttt
     1741 atggaaaaga cgatcgtgta caagcacact tattgattca gtcctttcga ccaggagaaa
     1801 ttaccgctga aaaagccaat caactagggg cagagtatgc gaaaaaaata gcccctaacc
     1861 accaaatcgc gatttacaca catacagaca aagaccatat tcataatcac attgtcatta
     1921 actccgttaa tcttgaaact gggaaaaaat ttcaagccca tgggcaagca tttttaaaca
     1981 aatgttatga cctaaatgac gagatttgtc ttgaacatga cttaacaatc acggaacgag
     2041 gaaagaaaac cgaaaaacga accatgtctg aaattaaatt aaaagaaaaa aatgaaccgg
     2101 tttggaaaga cgaaattcgg tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa
     2161 cgtataccca attttgtgat tcacttaaaa cattcggcat tcattgcttg aatcgcggaa
     2221 aaaattttac ttacgaattg gtccacaaaa agaaaaaagt acgatccaac aaactaggaa
     2281 aggattacga aaaggagacg attttacatg agctggacag acgagaacga acctacaatc
     2341 aacaacgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag
     2401 tacgaagaga acaacgaact tttacgcctg aacaatcgga aattagaccg actcgaagaa
     2461 accatgaaca acacgttgaa cgaggaacag accaccaaca agaattatcg ctctaaaacg
     2521 ttaaaagaca tcgaaaacga aataaagaga caacgtgcct ttcttgcgcc ttatacccaa
     2581 gaaaggatac aaactacgat ggattcacaa gtcaaaaaac tccaccaagc gctattagac
     2641 cagcgaaacc aagaacaaaa aaaggcttta attcaacgat cgatggatat tcttgccccc
     2701 ttatgttgct tagtactaac attattagcc ttttggttgg tatggggaga ataaacagac
     2761 atggcattct atttacccta tctactcatt tctgccagta ttttaggaat tatttggctt
     2821 agctataagg cttatcaaac aagatattca ttaaaaggct ctaaaattcg atttaagcga
     2881 tttttattgg tcggggttat atttgcctta tcaatcgttt taagtggttt attcggcgtt
     2941 ttagagggta ataaacgtgt ttcaagaagc attttattag gggatatcac tcaaaagtat
     3001 gaagcagcat gtgataaaaa gaaaaaagag caagcattag cacaaaaaat ggaggaattt
     3061 accgcttgtt atgaggacat gaacaatatt tttgtggatc aagagaaacg tttaacagat
     3121 aaaaacatga aaaaactcac tcgtttttat caaaacttac cagaaaaaca gcaaaaagag
     3181 gtacaagaaa aatatgagca agtaaaaaaa gacgtgcaat attttaaaga tacgcagaca
     3241 gaagaagctt gttgtaactt atttagcgat acaatccctt tttcaaccag tgaacaagaa
     3301 cgaaaagaac ggcaacaatc ggtcacatac gaacgttatg aaacactgct tcagcaagca
     3361 acaaacatac aaaatccaac gaaaaaagaa acggcattaa attatctaga aagtgtcaag
     3421 gaatggttgg atcagcaaca acaaaattga atttgattag caatcatgtt aaactgaacc
     3481 tgcaaatgat caaacattag atacacgaaa gccccagaga ggtcggattc tctggggctt
     3541 tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat tcaaccaata
     3601 actgaataaa gagacacaaa cttctacaag aagggggcaa atgaaccgtt caaacattag
     3661 atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat accttacaaa
     3721 ataaaatctg tgtggtgttt ttaagtatcc cttattttcg ggttgttagg gcttgccctg
     3781 accgcctgta aggcgcttta agaacaagat aaggtttttt acccaagact gtcttaaaac
     3841 gacttagacg agctatcaga ggcttccccc acctccgtta ctgacttgtt tttagcaaag
     3901 gagcctttag gcgactgcgc agtacaatgt gagagcatag cgatcacact tttttagcga
     3961 ccgtagggag caaggttttg aattggcagt tttgaaaaat gcgcgaacct gttggtagaa
     4021 aaaagaagtg gtttttaggc gggaaatttt gtcaccctct tttataatct ctttttaaac
     4081 ctttttttaa acctctttac cgtcggctgt gtccttactc cttcaatgga tacagcgttt
     4141 tataaactac attttgtctg cttttaaact acattttgtc tgcttttaaa ctacattttg
     4201 tctgctttta aactacattt tgtctgcttt taaactcttt ttttgcactc acgtatgtta
     4261 taatatgcat tatcacagac aaaacgttgt ttataacgtc tgtatccatt gaaggagtaa
     4321 ggaggaatat ctttggcaaa tgaaatcgtg aaataccatc atgaattaaa taccattccg
     4381 ttgcggaaat ttacaccagt tgaaatgaat ctatttttct ctattgtgtc tagaatgcgc
     4441 gaaaaaggcg ataaaacagt ccgtcttact tttgaccaac taaaagattt aagtaattat
     4501 aaagcaactg ctaataatcg tttcattaat gatttagaaa tgacttatga taaattaatg
     4561 gatttacgtt ttggtaaaag aagtgctaat gggctcgata tagatagatt cgttatgttt
     4621 agtgagttta aaatacgtgg aactgctaaa gatccatatg tagacataca agtttacgaa
     4681 aaagctttac ctttactaaa caatttagat gagtgggttc gctattcact ccaacaattt
     4741 aacgaattac aaagtagcta tgcaaagact atgtttcgat tactaaaaca atatcgaact
     4801 aaaggttttg cttatttttc taaagaggat tttcatgaac tattagatat tccaaaaagt
     4861 tactggaaca accaagcaaa cgttgataat cgtgttataa agcctatacg acaggaatta
     4921 accgcaatct tcaaaggatt atcaatcaaa aagaaatacg gaaaagggcg aggaaaacct
     4981 gtcgttggct atcaatttac gtttaaacca gaaatcaaaa aagcagatga ttttaataaa
     5041 catgcaccaa ccaaagaaga acaaaataaa gcattggctt tagctgataa acaaaaagaa
     5101 tacgaagaac ctgctacaac aattaacgaa aaaatcaaac agaagaaacg agaacttgaa
     5161 ctcctccaac aaatcaaaca actagaaaaa gagaaagaag ctcttgaaaa acaaacagag
     5221 tccgcacttt cg
//
LOCUS       DAHUWM010000179         5211 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHUWM010000179 DAHUWM010000000
VERSION     DAHUWM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.183"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(1273..1938)
                     /locus_tag="NK788_002309"
     CDS             complement(1273..1938)
                     /locus_tag="NK788_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770619.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="NK788_002310"
     CDS             2118..4100
                     /locus_tag="NK788_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCH6770620.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="NK788_002311"
     CDS             4104..4742
                     /locus_tag="NK788_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770621.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="NK788_002312"
     CDS             4770..5198
                     /locus_tag="NK788_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770622.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1719 a          796 c          952 g         1744 t
ORIGIN      
        1 aaaatgattt agaatatcaa aataacaaaa aagaggacta cctgtattcg tcctcttttt
       61 tgaccagatt ttgaccagtc taatttacat tctaatatat tcatatgagc taattttttg
      121 attaaatggt aagtttttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt t
//
LOCUS       DAHUWM010000180         5162 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHUWM010000180 DAHUWM010000000
VERSION     DAHUWM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.184"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            169..657
                     /locus_tag="NK788_002313"
     CDS             169..657
                     /locus_tag="NK788_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770623.1"
                     /translation="MELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAEL
                     QKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFP
                     QLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEK
                     AN"
     gene            716..1156
                     /locus_tag="NK788_002314"
     CDS             716..1156
                     /locus_tag="NK788_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770624.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1168..1836
                     /locus_tag="NK788_002315"
     CDS             1168..1836
                     /locus_tag="NK788_002315"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCH6770625.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1900..2523
                     /locus_tag="NK788_002316"
     CDS             1900..2523
                     /locus_tag="NK788_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCH6770626.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2579..2965
                     /locus_tag="NK788_002317"
     CDS             2579..2965
                     /locus_tag="NK788_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCH6770627.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3178..4569
                     /locus_tag="NK788_002318"
     CDS             3178..4569
                     /locus_tag="NK788_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCH6770628.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1711 a          861 c         1085 g         1505 t
ORIGIN      
        1 gttttaaaag atactctgat tgagcgatct acatttctat atgttcaatt gcaggaaaat
       61 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
      121 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
      181 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
      241 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
      301 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
      361 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
      421 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
      481 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
      541 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
      601 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
      661 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
      721 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
      781 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
      841 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
      901 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
      961 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     1021 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     1081 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     1141 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     1201 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     1261 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     1321 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     1381 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     1441 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     1501 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     1561 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     1621 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     1681 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     1741 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     1801 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     1861 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     1921 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     1981 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     2041 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     2101 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     2161 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     2221 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     2281 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     2341 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     2401 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     2461 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     2521 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     2581 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     2641 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     2701 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     2761 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     2821 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     2881 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     2941 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     3001 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     3061 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     3121 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     3181 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     3241 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     3301 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     3361 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     3421 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     3481 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     3541 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     3601 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     3661 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     3721 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     3781 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     3841 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     3901 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     3961 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     4021 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     4081 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     4141 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     4201 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     4261 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     4321 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     4381 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     4441 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     4501 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     4561 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     4621 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     4681 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     4741 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     4801 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     4861 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     4921 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     4981 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     5041 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     5101 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     5161 at
//
LOCUS       DAHUWM010000181         5127 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHUWM010000181 DAHUWM010000000
VERSION     DAHUWM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.185"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..287)
                     /locus_tag="NK788_002319"
     CDS             complement(<1..287)
                     /locus_tag="NK788_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770629.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="NK788_002320"
     CDS             429..1547
                     /locus_tag="NK788_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCH6770630.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="NK788_002321"
     CDS             1675..3051
                     /locus_tag="NK788_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCH6770631.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="NK788_002322"
     CDS             3452..5032
                     /locus_tag="NK788_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCH6770632.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT         1662 a          861 c         1050 g         1554 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttcc
//
LOCUS       DAHUWM010000182         5087 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHUWM010000182 DAHUWM010000000
VERSION     DAHUWM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.186"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(181..1329)
                     /gene="nagA"
                     /locus_tag="NK788_002323"
     CDS             complement(181..1329)
                     /gene="nagA"
                     /locus_tag="NK788_002323"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCH6770633.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1405..2241)
                     /locus_tag="NK788_002324"
     CDS             complement(1405..2241)
                     /locus_tag="NK788_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770634.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2425..3642)
                     /locus_tag="NK788_002325"
     CDS             complement(2425..3642)
                     /locus_tag="NK788_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCH6770635.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3791..3970)
                     /locus_tag="NK788_002326"
     CDS             complement(3791..3970)
                     /locus_tag="NK788_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770636.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            4025..4108
                     /locus_tag="NK788_002327"
     tRNA            4025..4108
                     /locus_tag="NK788_002327"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4059..4061,aa:Leu,seq:gag)
     gene            complement(4348..>5087)
                     /locus_tag="NK788_002328"
     CDS             complement(4348..>5087)
                     /locus_tag="NK788_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770637.1"
                     /translation="ISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGIS
                     SFGKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSF
                     TYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFD
                     NKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSM
                     SVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1567 a         1037 c          810 g         1673 t
ORIGIN      
        1 aaagctttta ttttgctaat acgtattaac atttttaatt ttatctttac attgttactt
       61 attgaacaat aggaaattaa agaaactttt gaaacttatg taaagtgatt tttactagaa
      121 cacaaaaaaa ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata
      181 ttatgcgtgg aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt
      241 caatacgata aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt
      301 acagctgatt gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc
      361 ccagtctaca acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc
      421 agattctaga cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt
      481 tccatcaggc atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc
      541 tgctttttcc attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa
      601 ttcagaaaag acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt
      661 caatccacgc ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt
      721 ttgcgcttct tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt
      781 cacttgtttg acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat
      841 taaaccacct gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata
      901 acttgggttt tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc
      961 tttgatttta gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt
     1021 taaacgttct ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa
     1081 cagaccttca gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat
     1141 atagccgtga atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat
     1201 gatgttgact tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat
     1261 ttcaagatag cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa
     1321 agttctcatt ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa
     1381 gaagtcaaga atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc
     1441 taagccttca tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt
     1501 aagcacaaat aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc
     1561 ttgattcacc tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata
     1621 attataaaaa atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag
     1681 ttggatgctt cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat
     1741 ttttccgata caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg
     1801 gctagagtag cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa
     1861 cgtgaattcg caaacaccga gataataata atagcgttga aagtcagttg cttgatacag
     1921 attttcagaa atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg
     1981 gaaacaacta tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag
     2041 attcatcaca tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac
     2101 attcggcagt tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg
     2161 cgaaagcatt ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa
     2221 ggactcgatt aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc
     2281 gtattttact ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt
     2341 tagttattta agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc
     2401 tactgaaaaa tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat
     2461 tttcttagct gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc
     2521 tggctggttc caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa
     2581 gtccaaaacg aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa
     2641 tatataaaaa gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt
     2701 atagatatat tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact
     2761 ttgataaccg cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg
     2821 catggaggac aacatattca gcccttctat atagcctttg acgtgtgact tatctccact
     2881 taggaccatc cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag
     2941 cgtaacaacg aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat
     3001 cactaagcga tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc
     3061 aacgatttct tctaataatt cttttggata aagtgcccca gtaggattat taggattaat
     3121 gataacaata gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata
     3181 ccattctgcc tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga
     3241 agccgtccac aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa
     3301 cccctgcata cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat
     3361 cgtcacattc ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa
     3421 gatccccttg gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat
     3481 gacttcgttt ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat
     3541 actaatgccc tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta
     3601 actgacacct tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca
     3661 tttcaaaaag ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca
     3721 tcctagaaaa ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag
     3781 cgttgagaac ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcaa
     3841 ttccacaaat gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca
     3901 atacacctaa gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa
     3961 gtcctcccat ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta
     4021 ttacgcagat gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct
     4081 cgtgtgggtt cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga
     4141 gtttttttgt ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa
     4201 aagaacgtac aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac
     4261 tcgtagaaac ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta
     4321 atactaaagc gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca
     4381 aataccaagc acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt
     4441 cattagctct ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta
     4501 cgtccgcatt ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa
     4561 tcccattatc atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata
     4621 aattccaaca attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc
     4681 tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact
     4741 actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac ccattgtatt
     4801 accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac gcacataata
     4861 gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata aaaataagat
     4921 attcatgtct aaaaaaaacg gctttccaaa tgatgaaata cctaaatcta acttattaag
     4981 aaagttgaaa tttttatatt caggaaacca tttattaatt tcatgtgcag cacgtaatgc
     5041 attccctgga gttgtcaaag agaaaattaa ttctgctaag cttatta
//
LOCUS       DAHUWM010000183         4989 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHUWM010000183 DAHUWM010000000
VERSION     DAHUWM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.187"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            24..842
                     /gene="purR"
                     /locus_tag="NK788_002329"
     CDS             24..842
                     /gene="purR"
                     /locus_tag="NK788_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCH6770638.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1345..2718
                     /gene="glmU"
                     /locus_tag="NK788_002330"
     CDS             1345..2718
                     /gene="glmU"
                     /locus_tag="NK788_002330"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCH6770639.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2812..3783
                     /locus_tag="NK788_002331"
     CDS             2812..3783
                     /locus_tag="NK788_002331"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCH6770640.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3789..3891
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3957..4526
                     /gene="thiT"
                     /locus_tag="NK788_002332"
     CDS             3957..4526
                     /gene="thiT"
                     /locus_tag="NK788_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCH6770641.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4607..>4989)
                     /locus_tag="NK788_002333"
     CDS             complement(4607..>4989)
                     /locus_tag="NK788_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770642.1"
                     /translation="QLNRIYYRYLDEFYRIFASKLSSILSDKINVKKSFFKKETPVEK
                     ISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIAT
                     LIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1626 a          883 c         1083 g         1397 t
ORIGIN      
        1 tttcttaaaa ggagttggaa taggtgaaag tacgtcgcag tgaacgtcta gttgacatga
       61 cacagtattt attgaatcac ccgcatgaat tgatttcctt aacaagcttt gccgaacgtt
      121 atgaatctgc taaatcttct atcagtgaag atttgggaat catcaagaaa acatttaaag
      181 aacgagggta tggaaccttg gaaacaatcc cgggtgctgc aggtggtgta ttgttcatcc
      241 ctgaaatttc atatgaagag gcgaaaaaat atattgaatc tctagcagaa cgtctatcag
      301 aacaagaccg tctgcttcca ggaggatatg tttatctctc tgatttgtta ggagaacctg
      361 aactgctgcg tcaagttgga agaatcattg cttctaaata ccttggtgaa aaaatcgatg
      421 ctgtaatgac tgtagccaca aaaggcgtac ctattgccca agctgtgtcc tattatctga
      481 atgcgccatt cgttattgta cgccgggact caaaaataac agaaggatca acagtaagtg
      541 tcaactatgt gtctggatct tctgaaagaa ttgaaaagat ggaactgtct aagagaagtt
      601 tgaaacgtgg ttcacgtgtc cttgtagtgg atgatttcat gaaaggcggc ggaacagtca
      661 acggaatgaa gagcttgatt gaagaattcg aatcagaact tgttggcatc actgtatttg
      721 ccgaatcaaa attcaacggt catcgcgcca tcgatgatta tacgtcactg ctttatgtca
      781 aagatgtaga tactcaaacg aaaaatatta cggttgtgcc aggaaattat ttttcagaat
      841 aagaggttgt gaaaaagccg cttagtttcg agtagtaaga aaaattttgt gaaaatggct
      901 ttccacattt tttcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
      961 tctagaggtt gtgaaatcag cgtcctgtcc tgagtattag ctcaataaac ggaaaaattg
     1021 ctcctcatat tttgccgttt attgagctaa taccgaaggt cagctgattg aaaaacgttt
     1081 aatgaagagg ttgtaagcct accgtttaac tctaagtcac aagaaacgat ttcaaaaaac
     1141 agcttttcat gttttatgag tagtgtgact tgtgaccgag gagttggcag gagagcaccg
     1201 gttaccaaat agataggccc gttatggtct atctattttt ttatccagaa aacgctggga
     1261 taaaagaaaa tcacttgttt tgttgaaaaa atgggtatac tgggataaac tgaaatcagc
     1321 aaactatatt taggagtgac gcagttggac gcacgttatg caattatttt ggcggcaggg
     1381 aaaggtaccc gcatgaagtc aaaattatat aaagttttac atcccgtttc tggtaaacct
     1441 atggtagaac atatcattaa tcgggtcagt gaaacgaaac cagatgaagt gattacgatc
     1501 gttggacacg gagcagaaca agttaaagca caactaggag aacgcagtaa atatgcgtta
     1561 caagcagaac aattgggaac aggacatgct gttttgcaag ctgcttcttt cttggaagga
     1621 aaaaaaggaa caacgttagt tatcagtggc gatacaccat tattgactac tgaaacgctg
     1681 aacaatcttt ttgaatatca ccaaggaaaa aatgcaagtg caacgatctt gacggcacaa
     1741 gcagaaaacc caacaggata tggacgaatc atccgtgacc atattgggat cgtagaaaaa
     1801 atcgtggaac aaaaagatgc aacaccagaa gaagctcttg tacaagaaat caatacagga
     1861 acttactgtt ttgacaacga agctttgttc gatgcgttaa gcaaagttgg aacaaacaat
     1921 gctcaaggtg aatactatct aacagatatc atcgaaatct taaaagaaga aggccataca
     1981 gttgctgctt atcaaacgga tgattttgaa gaatcaatgg gagtcaatga tcggatcgcg
     2041 ttagctaaag caaatgagat catgagaaag cgaattaacc agatgcatat ggtcaatggc
     2101 gtaagctttg tcgattcagc aacgacatac atcgatgcag gcgtagaaat cggtccagac
     2161 acattgatcg aagcaggtgt acaaatccaa ggaaatacag tcattggttc tgattgtgtc
     2221 atcgggtcac attcaaaaat cgtagacagt aggattgagg atcacgtcgt cattgaaaac
     2281 tcagtcattg aaagcagcca tgtcaagaaa catgcagatg tgggacctta tgcacattta
     2341 cgaccaaaag ctgaaattgg cgaaaacgtc catatcggaa actttgtgga agtgaaaaat
     2401 gctcaaatcg gcaaaggaac taaagtcggt cacttgactt atgttggtga tgctacgctg
     2461 ggagaagaaa tcaatgtcgg atgtggtgtt gtcttcgtca actatgatgg aaagaacaaa
     2521 catcatacga ctgtcggcga ccacagcttt attggttcta gcacgaatat catcggacct
     2581 gtcgaggtag ctaaaaacag ttcgattgct gccgggtcaa caattacaga caatattcca
     2641 gaatatgcgc tggcaattgc gagagcaaga caagtcaata aagaaggata tgcaaaaaaa
     2701 ctcccttatc taaactaaga cttttgcaaa atcacttgat ttatttaaaa aatatgacta
     2761 ctatttatta ggtagaattc tagagttgtg aaaaagaaag tggaggtccc catgtcaaaa
     2821 cattactttg acccaagatt aaaaattttt gcgttaaatt ccaatcgccc attagcagag
     2881 aaaattgcag cagcagtcgg cgtcgagtta ggaaaatcat ctgttactca atttagtgat
     2941 ggagaaattc aagtaaacat tgaagaaagt attcgtggat cgcatgtcta tgttattcaa
     3001 tcaacgagca gcccagtcaa tgataacttg atggagttgc tgatcatgat tgacgcactg
     3061 aaacgagcaa gtgcaaaaac aatcaacgta gtcatgcctt attatggcta tgcgcgtcaa
     3121 gaccgtaaag cacgtgcacg ggaaccaatc acagcgaaat tagtagcaaa catgattcaa
     3181 aaagctggtg caacaagaat gctgactttg gaccttcatg cagtccaaat ccaaggtttc
     3241 tttgatattc cagtcgatca tttgatgggt gcgccactta ttgccaatta tttcttagaa
     3301 aaaggaatca aaggcgatga tgttgtcgtt gtttcaccag accatggtgg tgtcacacgg
     3361 gcacgtaaat tagcggaatt tttgaaatct ccgattgcga tcatagacaa acgccgtcca
     3421 aaagcaaatg ttgcagaagt aatgaatatc attggacaag tcaaaggcaa aacttgtgta
     3481 ctgatcgatg atatgatcga tacagcaggt acgatcacat tggctgccaa tgctttgaaa
     3541 gaagctggtg caactagtgt gtatgcttca tgtacacatc cagtattatc cggccctgct
     3601 ttacaaagaa tagaagattc agctatcgag cgtctagttg ttacagactc gatctatctt
     3661 ccagaagatc gtaaaatcga caaaatcgat gaagtaagtg ttggcggact aatgggagat
     3721 gcaatcaaac gtatccatga aaacaaacca gttagtccat tgtttgaaac aaaaaataaa
     3781 taataaataa actcacaaag gggagtccaa ttgggctgag attgaatcta tttctaaacc
     3841 cttcgtacct gtatcggtta tgcgagcgta ggaattgtga ataaacagct gtttgactat
     3901 tttcgaaagc tgcttcctcc tttgtgactt ttttattcaa aggaggaagt ttttttatgg
     3961 gaaaaaatag agtttgggta gaaggaacga ttgttgcagc gttagcgatg gtgttgtcgt
     4021 tgattccgat ccagatcggc agcagttttt ctatttcatt agggcagatt ccattgactc
     4081 tttttgcgtt aagaagaggg acaaaggcag ggatgctggc aggtttcatt tggggcatcc
     4141 tacattttcc gttgggacaa gtttattatt taagtgtcgt tcaagtgttg attgagtacc
     4201 cgattgccta tacttttgca ggcgcagcag ggctcatggc ttcaaatgtc caaaaaggct
     4261 atgtagacga taatacgaaa caaattagaa aaagcatcgt gtttggtgca ttgattggcg
     4321 cactttccag atatttttgg cattttattg caggagtggt tttctgggga gcgtatgcac
     4381 tatgggggat gaatccttgg ctcttttctt tcgtaatgaa tggtgccagc gggttgagta
     4441 cagctattgt aacgatcatc gttttattag ggatccaaca aacgactcct tcgatttttg
     4501 tgccagataa acgacaggag ctgtaaataa aaaagaagca agaaacttga gacagaatca
     4561 tgtcctagtt tcttgtttcc tttttatttt ttgataagaa atagatctac ttcaatggca
     4621 gataagaaag atcggcatct tctaccaatt gccagtgtcg atcggtgaca gagtagatta
     4681 aagtagcgat gatttcctct tctcgatacc gggtgccttt taatttccaa atataatctt
     4741 caatcacata gattgggata cgatatttac catcgatcaa accaacttga tcaaaagaaa
     4801 aaatgatccc ttcctgcatc aaattacgca tttctttcaa taattccaca ctgatttttt
     4861 caacaggtgt ttcttttttg aaaaaagatt tttttacatt gatcttatct gacaagatcg
     4921 aacttaattt actagcaaaa atcctataaa attcatctaa atagcgataa taaatccgat
     4981 tcagctgat
//
LOCUS       DAHUWM010000184         4962 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHUWM010000184 DAHUWM010000000
VERSION     DAHUWM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.188"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            33..455
                     /gene="mgsA"
                     /locus_tag="NK788_002334"
     CDS             33..455
                     /gene="mgsA"
                     /locus_tag="NK788_002334"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCH6770643.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            778..1314
                     /locus_tag="NK788_002335"
     CDS             778..1314
                     /locus_tag="NK788_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCH6770644.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(1354..3117)
                     /locus_tag="NK788_002336"
     CDS             complement(1354..3117)
                     /locus_tag="NK788_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770645.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3120..4835)
                     /locus_tag="NK788_002337"
     CDS             complement(3120..4835)
                     /locus_tag="NK788_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770646.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         1507 a          969 c         1006 g         1480 t
ORIGIN      
        1 gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc
       61 gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg
      121 aactttttgc taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc
      181 atcggtttaa atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg
      241 atcagttaga tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag
      301 atgtaacagc acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg
      361 gaacagctga ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg
      421 tccatgaaat agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg
      481 tttaatgttc gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat
      541 acgaacgtat tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga
      601 aaatatgcta tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag
      661 cgttaagtgc taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt
      721 ttattttcgc attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg
      781 aagaaacacc ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg
      841 gtcactttgt cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt
      901 attccacaag tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt
      961 ttagctgatt tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca
     1021 ttgaatgcat ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact
     1081 tggaaaaatg ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca
     1141 ccattatggc ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc
     1201 cgcctgttaa aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga
     1261 cgtagtattc cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca
     1321 agcagtcttc ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata
     1381 gagccgtcgg taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat
     1441 agctccctga tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc
     1501 aatcacaaaa ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc
     1561 tgtcaatgta tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat
     1621 catcgttcga gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc
     1681 gccaatctta gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc
     1741 ttttagggca tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc
     1801 tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa
     1861 tgtatcacgt tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt
     1921 gtcataaaaa cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac
     1981 tatagcaatg gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt
     2041 ggggtcataa gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc
     2101 caaggcatag gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc
     2161 tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa
     2221 tggtttagaa aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga
     2281 cgaaccactc ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg
     2341 atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt
     2401 ttcatataga tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa
     2461 agctttcacg attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat
     2521 aatcctttgc tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag
     2581 gaaaataatt ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat
     2641 aaaaataaac gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga
     2701 aattgcaatg gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga
     2761 gttttgatca taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa
     2821 ttttgcaacg gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac
     2881 tacgttgatt aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg
     2941 gacctgtcct tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac
     3001 tgttgcaatc ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc
     3061 agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac
     3121 tattcttcct cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct
     3181 tcatgggtac cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct
     3241 tgaatagagc ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc
     3301 gccttacgca aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc
     3361 agtaatgctg gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca
     3421 gagaaatttt tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca
     3481 aattcttctg cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc
     3541 ttaccccatt gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca
     3601 agagcaattt gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt
     3661 ataactgtac cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc
     3721 ccgcttccgg ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata
     3781 ttttggagag aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata
     3841 attgcttcag aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct
     3901 gtgtctagca cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg
     3961 aggtttgaaa caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga
     4021 acttgtcctt gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag
     4081 atgatcccga cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa
     4141 acacgtaaat aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt
     4201 tttgtctgcg caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca
     4261 ttgagctgat ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata
     4321 aaaaataaaa tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag
     4381 tcgatcacaa aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa
     4441 cgaatgacca ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc
     4501 aaggtatctg ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt
     4561 agctgattac gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa
     4621 atgatagcac aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg
     4681 atttttcctc ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc
     4741 aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta
     4801 cgatagtctt ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt
     4861 ccacaattga aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag
     4921 aacaagcctt ttcataaatg aaagttttat tatgaagaag ct
//
LOCUS       DAHUWM010000185         4911 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHUWM010000185 DAHUWM010000000
VERSION     DAHUWM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.189"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..258)
                     /locus_tag="NK788_002338"
     CDS             complement(<1..258)
                     /locus_tag="NK788_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770647.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="NK788_002339"
     CDS             complement(274..924)
                     /locus_tag="NK788_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770648.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="NK788_002340"
     CDS             complement(935..2107)
                     /locus_tag="NK788_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCH6770649.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="NK788_002341"
     CDS             complement(2126..2998)
                     /locus_tag="NK788_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6770650.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="NK788_002342"
     CDS             complement(3020..3940)
                     /locus_tag="NK788_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770651.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="NK788_002343"
     CDS             complement(3940..4209)
                     /locus_tag="NK788_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770652.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="NK788_002344"
     CDS             complement(4206..4586)
                     /locus_tag="NK788_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCH6770653.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1307 a         1068 c          802 g         1734 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc t
//
LOCUS       DAHUWM010000186         4891 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHUWM010000186 DAHUWM010000000
VERSION     DAHUWM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.190"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(201..1184)
                     /locus_tag="NK788_002345"
     CDS             complement(201..1184)
                     /locus_tag="NK788_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCH6770654.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1189..1836)
                     /locus_tag="NK788_002346"
     CDS             complement(1189..1836)
                     /locus_tag="NK788_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCH6770655.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1921..2553)
                     /locus_tag="NK788_002347"
     CDS             complement(1921..2553)
                     /locus_tag="NK788_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6770656.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2569..3120)
                     /gene="coaC"
                     /locus_tag="NK788_002348"
     CDS             complement(2569..3120)
                     /gene="coaC"
                     /locus_tag="NK788_002348"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCH6770657.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3117..3854)
                     /locus_tag="NK788_002349"
     CDS             complement(3117..3854)
                     /locus_tag="NK788_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCH6770658.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3851..4840)
                     /locus_tag="NK788_002350"
     CDS             complement(3851..4840)
                     /locus_tag="NK788_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCH6770659.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         1395 a         1012 c          960 g         1524 t
ORIGIN      
        1 attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
       61 tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg
      121 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
      181 cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg
      241 tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg
      301 attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg
      361 agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt
      421 ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc
      481 aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg
      541 atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga
      601 aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt
      661 ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg
      721 atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt
      781 aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca
      841 gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca
      901 tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg
      961 gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag
     1021 ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta
     1081 tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct
     1141 aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt
     1201 tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt
     1261 acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt
     1321 gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc
     1381 gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc
     1441 atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc
     1501 atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat
     1561 cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc
     1621 agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt
     1681 taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc
     1741 tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc
     1801 aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa
     1861 tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta
     1921 tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt
     1981 cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc
     2041 tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa
     2101 gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa
     2161 taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata
     2221 aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc
     2281 atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat
     2341 aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct
     2401 tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg
     2461 accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat
     2521 cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct
     2581 ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt
     2641 tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga
     2701 tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc
     2761 aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc
     2821 gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa
     2881 caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat
     2941 gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg
     3001 tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt
     3061 aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat
     3121 tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga
     3181 gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt
     3241 tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg
     3301 actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct
     3361 tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca
     3421 agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca
     3481 aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct
     3541 tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg
     3601 tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc
     3661 gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa
     3721 tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct
     3781 gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg
     3841 attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag
     3901 caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt
     3961 catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta
     4021 cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg
     4081 cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca
     4141 atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct
     4201 gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag
     4261 cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact
     4321 ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag
     4381 taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca
     4441 tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta
     4501 gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga
     4561 cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac
     4621 tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct
     4681 gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta
     4741 ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa
     4801 caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa
     4861 aattttcttt tatcatacca taaagcctac t
//
LOCUS       DAHUWM010000187         4801 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHUWM010000187 DAHUWM010000000
VERSION     DAHUWM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.191"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..2232)
                     /locus_tag="NK788_002351"
     CDS             complement(<1..2232)
                     /locus_tag="NK788_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6770660.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2711..2977
                     /locus_tag="NK788_002352"
     CDS             2711..2977
                     /locus_tag="NK788_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCH6770661.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            2977..4704
                     /gene="ptsP"
                     /locus_tag="NK788_002353"
     CDS             2977..4704
                     /gene="ptsP"
                     /locus_tag="NK788_002353"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCH6770662.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         1467 a          903 c          976 g         1455 t
ORIGIN      
        1 ttcagccgtt tcttcggctg attctttagc caaacgttct ggtttagaag taacgatgat
       61 ttttccatct ttatccagtt ttgctttcaa ctcatggata gaagggtgat caagataaaa
      121 ttcagcgata ccatcttcga tttgttcttg gatcgtgcgt cgaagaggac gagcacccat
      181 agatggatca tagcctaagt caactaattt ttctttgaca ttcgtaggaa cttcgatgtg
      241 aagttgctgt gctgccagca tttggttgac atcgtctagc attagtgtga cgattgtcat
      301 caagttttct tttgataatg cactgaattc gatgatcccg tcaaaacggt tcagaaattc
      361 tggtgtaaag tagttgttta attggttcaa tacagaacga gtaacacctt cgcgtgccgc
      421 accaaaacca acgtttgctt ctactttacc agttccggca ttacttgtca taatgatgat
      481 cgtatcttta aagctgaccg ttcttccttg tgcatctgtc aatcgaccat catctaagat
      541 ttgcaagaac atatgcaaaa catctggatg agctttttct acttcatcta acaagacaag
      601 actgtaagga tttctccgta ctttttctgt taattgacca gcttcttcgt atccgacata
      661 acctggagga gaaccaatca atttagagac actatgtttt tccatatatt cagacatatc
      721 gaaacgaatc attgaatctt gtgaaccgaa tagttcgtag gcaagttgtt tcgcaagctc
      781 tgttttacct acaccagttg gtccaacaaa taagaaggag ccgattggac gattctgttt
      841 gtttaatcca acacggtttc ggcgaatcgc tttcgctact cgatctacag cgttatcttg
      901 tccgatgaca tgagctttca agtcatctgc taagtttttc agttgtgtct gttctttttc
      961 ttttaattca cccactggaa tacctgtccg ttgttcaacg attttttcca tatctttttc
     1021 ggtaatcaca ggtgtttcct cttcagtgag ctgacgttct ttcatttttt gaagtttatt
     1081 gatctgatcg cgataataag ctgctttttc gaaatcttct tctcgagaag ctaaaacttt
     1141 ttgttcttct gcttcttgca gttttttctc gattgtttta ggatcaacga tttggatcgt
     1201 caagtttttc ttagaacctg tttcatctag caaatcgatg gctttatccg gcaagaagcg
     1261 atcttggacg taacgattgg ataaagttgc ggctgcttcg atggcctcat cagtatattt
     1321 tacatgatga tagtcttcat aacgtttttg caaacctttc aaaatactga tcgtttcttc
     1381 gacagttggt tcatctaccc gtacaggttg catacgacgt tctaaagcag cgtctttttc
     1441 gatgatgcgg tactcattta aggtagtagc accaaccatt tgcaattctc ctcgagctaa
     1501 agctggtttt aaaatattac cagcatccat atttccatca ccagcagaac cagcaccgac
     1561 gatttcgtga acttcatcta tgaataggat cacattttct gcttgacgga tctcttcaat
     1621 caatttttgc atacgttctt caaattgtcc tcgtatacca gtaccttgga ctaaagatac
     1681 gacatctaaa cgaatgactt ctttgtccat taatttttgc ggtacatcac catctacgat
     1741 tttttgtgct aaaccttcaa caactgctgt tttaccaaca cctggttctc caatcaagac
     1801 aggattgttc ttggttcggc gatttaaaat ttcaatgact cgtttgattt cgtcatcgcg
     1861 tccgatcaca ggatcgatat caccttgtcg tgcttgttct gtgatattga ttccgtattc
     1921 tcccaataaa ccttctgtat tgccttgagg tggctgacca ccattgaagt tgttgtttcc
     1981 gccaaattgt gttggtggtg tttgctcata atttccttgc tgttgcattt ggcgagacat
     2041 ggaacggaaa agatcgtcca aactgccaaa tccaaatgga tcattttggg ccatattagt
     2101 cagacctcca ttttgttgat ttttgatttt ttgataacag ctctgacagt aatcgagttg
     2161 tgtccgctga ccattgacag tggcatacaa gtgaatcgtt gcctcattct tgccgcagtt
     2221 ttgacaaagc atgaatcatt cacgtccttt cacaaaaatg ggtacatcca tattttacat
     2281 ctcgtttttc atgaggtaaa agaataaccc ctagtttttt aagagattta tgaggtagaa
     2341 aaggtgaaat ctctattgac cattcctgac ctttgactct tattatacta aaaataaaaa
     2401 gggaagcaag taattcgttt catatgaaaa caaagagggt caaaggaaag aatgataaac
     2461 gtcaggagaa gagaaagcga caagaaaaag gaaataaaca cgtttgttat aaaatgtgaa
     2521 gaaaaggatg aaaaagaatc cggaataatt tgaatacttt tatctgaaat tgaatgaaaa
     2581 ctgttgtatc taacgaaaaa aaatgttaat ctttacagat gaaagggtgg catttgttag
     2641 gattctctga attactaaaa aagaaaaccc tcacataaaa acaacactca cttaaaggag
     2701 accgattaat atggaaaaga aagaatttca cgtagtagca gaaactggga tccacgctcg
     2761 tccagctaca ctattagtac aaacagcaag caaatttaac tctgatgtaa acttagagta
     2821 taaaggtaaa tcagtaaact tgaaatcaat catgggcgtt atgtctttag gtgtaggtca
     2881 aggttctgat gttactatta ctgcagaagg tgctgacgaa gctgatgcaa tggcagcaat
     2941 cgttgaaaca atgaagaaag aaggcttatc tgaataatgg ttgaaatgct aaaagggatt
     3001 gccgctagtg acggtgtagc cgttgcaaaa gcttacctgc tagttcaacc ggatttaaca
     3061 ttcagtaagg caacagtaga agatactgcg gctgaagaag ctcgtttgga tgctgcatta
     3121 gctaaatcta ctgaagaatt gcagcaaatt cgtgaaaaag cagcgcaaag cttaggtgaa
     3181 gcagaagcgc aagtatttga tgcccatttg atggttcttt cagatccaga aatggtaggt
     3241 caaatcaagc aaaatattaa agacaacagc gtaaatgctg aatcagcgct aaaagaagta
     3301 acggatatgt atatcggtat gttcgaagca atggaagata atgcttatat gcaagagcgt
     3361 gctgcagata ttcgtgacgt tgctaaacgt attttagcgc acttattagg cgttacttta
     3421 ccaaatccat caatgatcaa cgaagaagtg gtcgttgttg cacatgactt gactccaagt
     3481 gatacagcac aattagatcg caattttgta aaagcgtttg taacagacat tggtggacgt
     3541 acttcacatt ctgctatcat ggcacgttct ttagaaattc cagcaatcgt tgggacaaaa
     3601 gaaattactg ctaaagtcaa agaaggcgat attttggctg tcaacgggat cgaaggcgat
     3661 gtaatcatcg atcctacaga tgaacaaaaa gctgaatttg aaaaagcagg tgcagattac
     3721 gcagcacaaa aagcagaatg ggaaaaatta aaaaatgcag aaactgtcac agctgacggc
     3781 aagcattttg aattagctgc aaacatcgga acaccaaaag atctagttgg tgtccataat
     3841 aatggtggag aagcagttgg tctgtaccgt actgaattcc tgtatatgga ttctccagac
     3901 ttcccaacag aagatgacca atatgaagct tacaaagctg tattagaagg tatggaagga
     3961 aaacctgtag ttgttcgtac aatggacatc ggtggagata aagaactacc atatctgcaa
     4021 ttacctcatg aaatgaatcc attcttagga tatcgtgcat tgcgtatcag tttatctgaa
     4081 caaggcgacg aaatgttccg tacacaaatg cgtgcattgc ttcgtgcatc tgttcatggt
     4141 aatttacgta tcatgttccc tatggtagct acactcaaag aattccgcgc agcaaaagcg
     4201 atctttgaag aagaaaaaca aaaattgata agtgaaggca aggaagtatc tgatacgatc
     4261 caagtcggca tcatgatcga gattccagca gcagcagtct tagcagacaa gtttgcaaaa
     4321 gaagtagatt tcttctctgt aggtacgaac gatttgattc aatacacaat ggcagcagac
     4381 cgtatgaacg aacgcgtttc ttacttgtac caaccatata acccatcaat tttacgtttg
     4441 atcaaaaatg taattgatgc agcacacgct gaaggaaaat gggcaggtat gtgtggagaa
     4501 atggctggag atcaaacagc tgttccattg ctagtaggta tgggtctaga tgagttctca
     4561 atgagtgcga catctattct taaaacgcgt agtttaatga aacgcttgga cacagcgaaa
     4621 atggctgaac ttgctgatcg tgcattgaaa gaatgcgata tgatggaaga agtcgttgaa
     4681 cttgttcatg aatatgtaaa ataaacaacg tatcaaaagc tggtgtggct tgtaaaagag
     4741 ctacgccagt ttttttgttc ttttttaaga aaacgttttt gtcagtaaga atagacaaaa
     4801 a
//
LOCUS       DAHUWM010000188         4788 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHUWM010000188 DAHUWM010000000
VERSION     DAHUWM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.192"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            564..989
                     /locus_tag="NK788_002354"
     CDS             564..989
                     /locus_tag="NK788_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770663.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="NK788_002355"
     CDS             1019..1183
                     /locus_tag="NK788_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770664.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="NK788_002356"
     CDS             2141..3118
                     /locus_tag="NK788_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCH6770665.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="NK788_002357"
     CDS             3128..3625
                     /locus_tag="NK788_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770666.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="NK788_002358"
     CDS             4001..4282
                     /locus_tag="NK788_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770667.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="NK788_002359"
     CDS             4284..4700
                     /locus_tag="NK788_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770668.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAHUWM010000189         4758 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHUWM010000189 DAHUWM010000000
VERSION     DAHUWM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.193"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            72..1118
                     /locus_tag="NK788_002360"
     CDS             72..1118
                     /locus_tag="NK788_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCH6770669.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="NK788_002361"
     CDS             1135..2358
                     /locus_tag="NK788_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCH6770670.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="NK788_002362"
     CDS             2447..3334
                     /locus_tag="NK788_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCH6770671.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="NK788_002363"
     CDS             3420..3650
                     /locus_tag="NK788_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCH6770672.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="NK788_002364"
     CDS             complement(3707..4672)
                     /locus_tag="NK788_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCH6770673.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1606 a          791 c          968 g         1393 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttctgttc
//
LOCUS       DAHUWM010000190         4711 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHUWM010000190 DAHUWM010000000
VERSION     DAHUWM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.194"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            106..357
                     /locus_tag="NK788_002365"
                     /pseudo
     CDS             106..357
                     /locus_tag="NK788_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="NK788_002366"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="NK788_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCH6770674.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="NK788_002367"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="NK788_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="NK788_002368"
     CDS             1786..1869
                     /locus_tag="NK788_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCH6770675.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="NK788_002369"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="NK788_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCH6770676.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="NK788_002370"
     CDS             complement(2994..3857)
                     /locus_tag="NK788_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCH6770677.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="NK788_002371"
     CDS             complement(3859..4131)
                     /locus_tag="NK788_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6770678.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="NK788_002372"
     CDS             complement(4148..4357)
                     /locus_tag="NK788_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCH6770679.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="NK788_002373"
     CDS             complement(4455..>4711)
                     /locus_tag="NK788_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCH6770680.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DAHUWM010000191         4677 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHUWM010000191 DAHUWM010000000
VERSION     DAHUWM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.195"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="NK788_002374"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="NK788_002374"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCH6770681.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="NK788_002375"
     CDS             1085..2005
                     /locus_tag="NK788_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6770682.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="NK788_002376"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="NK788_002376"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCH6770683.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..>4677
                     /locus_tag="NK788_002377"
     CDS             3311..>4677
                     /locus_tag="NK788_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770684.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEK"
BASE COUNT         1634 a          755 c          908 g         1380 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatt
//
LOCUS       DAHUWM010000192         4670 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHUWM010000192 DAHUWM010000000
VERSION     DAHUWM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.196"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(255..1328)
                     /locus_tag="NK788_002378"
     CDS             complement(255..1328)
                     /locus_tag="NK788_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCH6770685.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(1325..2152)
                     /locus_tag="NK788_002379"
     CDS             complement(1325..2152)
                     /locus_tag="NK788_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770686.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(2153..2959)
                     /locus_tag="NK788_002380"
     CDS             complement(2153..2959)
                     /locus_tag="NK788_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770687.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(2971..4056)
                     /locus_tag="NK788_002381"
     CDS             complement(2971..4056)
                     /locus_tag="NK788_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770688.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(4073..4615)
                     /locus_tag="NK788_002382"
     CDS             complement(4073..4615)
                     /locus_tag="NK788_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770689.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         1422 a         1015 c          762 g         1471 t
ORIGIN      
        1 ttgcatagtt tccaattact ttatccttct gtaacttttt caatcttaga tttactagcc
       61 cgtctcgggc cctattttac aatggttttg tccacttttc tctggtagaa aaacgtggta
      121 gaaaaaataa aataacacaa aaaaagcggc gtttcatccg cttttggtag aagattggaa
      181 aactcagata gaaaaaggac gatttttagc tctttagaac gttgataagt ccccattctt
      241 actgagtttt gccattatct cctatacatc ttgaattcta agaataggtc attatagatg
      301 cctaggtatt tcgatcctag atctttgtag acttcaagat gggagatcgt gtcatcagat
      361 ggatagaatt gttcgtccga cgaaatgtct tttggcaata acgcttttgc ttcttcattt
      421 ggtgtagaat aaccaatata ctctgcattt tgtgccgcat tttccggttc tagcataaag
      481 ttgatgaaat cataggcacc ttctttattt ttcacggttt ttggcataac gatattatca
      541 aaccaaagat tggatccttc tggtgggatc acataatgta aatgttcatt ttcccacatc
      601 atatcggcag cttcgccaga aaatgtcact gccacggagg cttcttcatt gatcatatac
      661 attttgatct catctgccac gattgctttg acatttggtg ttaaatgatt caatttattt
      721 gctgcttctg tcaactgtgc catattctta ctattcaaag aatagcctaa actattcaaa
      781 gaaagcccca taacttctcg tgcaccgtca atcagcatca aactgtcttt taattccggc
      841 ttccacaaat catcccaatg cttaatttga tttgcatcaa agaatttatc gttatatacg
      901 atccctaaag ttccccaaaa atatggaatc gaatactgat tatctggatc aaaatccaag
      961 tctaaaaatc gtggatcaat attatttaat cctttgatct tcgaatgatc taatggaagt
     1021 actagttttt ctttgatcat tttttggatc atatattcac ttggtatagc gatgtcgtag
     1081 tttgtcccac cttgctggat ttttgtgaac atcgcttcat tagaatcaaa tgtttcataa
     1141 ttcactttgt atccatattc tttttcaaat ttcgtgatca aactaggatc gatataatct
     1201 ccccagttat agatggtaag tacctttgct ccactcatgc cacttgtttt ttcaagttga
     1261 cgaacaccaa gcagcagcac cgcaataatg atgagaatac ctaaaaacag agattgtaat
     1321 tttttcatcg cagccgtgcc accccctcgc gtactcttct gtttttcttc agccgttttg
     1381 gacggttttc tttactaata aagtagtatc caatgaccaa tatcatggag aacaagaaaa
     1441 ctaacgcact tagcgcattg atttctaaac tgattccttg tcttgcacga gaatagatct
     1501 ctactgagag cgtcgtaaag ccatttcctg tgacgaaaaa tgtaaccgca aaatcatcaa
     1561 gagaataggt aaatgccata aaatatccag ctatgattcc tggagaaaga taaggcaaaa
     1621 tgatattttt gactacttgg aaattattag caccaaggtc tctagccgca tcgaccattg
     1681 aatcgttcat ttcctgcaat tttggcaaga ccatcaagac gacgattgga atgctgaaag
     1741 caatatggga aagcaataca ctctgaaaac ctaatccgaa tcctagtagt gtaaagaaaa
     1801 tcaagaaact agcaccgata ataacatcag gagagactaa taagatgtta ttcatgctca
     1861 acaacgctgt ccgtgcacgt cgttttttcg tgtaatagat tcccatagcc ccaaatgtcc
     1921 cgatgatcgt ggcgatcaat gcagataaga aagccagcat gaacgtattc aatacgataa
     1981 tgatcaaacg tgtatcttca aaaacagctt cataattttc ccaagtaaat cctgtaaagc
     2041 tgttcatcgt tcctccttca ttgaaggaat aaaaaatcag atagaagatc ggtgcatata
     2101 agaataaaaa gacgatccct aaataaagat acgaccattt aaattttttc atttatgagc
     2161 gacctccttt tttcttctcg cctgtcagga gcatcacgat gaacatagca atgattaaaa
     2221 tcacaccgat tgtagaaccc atcccccagt tttgtgtaac caagaaatgc tgttcgatag
     2281 ccgttcctaa agtgatcacc cgatttccgc caatcagacg agtcagcatg aataaagaaa
     2341 gagaaggaat aaatactgct tggacaccac ttttcacacc atttaatgat aacggaaaaa
     2401 tcactcgtcg gaaggtctca aagctattcg cgcctaaatc tctactagca ctgatcaaag
     2461 aaggattcat ctcttccaat gcattgaaaa ttggcataat cataaatgga atctcgatat
     2521 acgttgccac gaacagaaaa ctgaagtctg tgaataatat ctgtttcggc ccgatcccga
     2581 taaaatcaaa gaaatgattc acgctgccgt gcacactgaa aatcccaata aatgcgtaag
     2641 ctttaagcag cagattcacc catgtaggca aaataatcag catcagccat aattgcttgt
     2701 gcttcagttt tgttaaaaaa taagcagtcg ggtaactgat gatcagcgtg aaaaacgtga
     2761 tcaaaaatgc ataccataca gaattgatcg tcatagaaag cgatgtcccc gacgtaaaat
     2821 aagatagata attattcagc gtgaattgcc cattcatatc aaaaaaagat tgataaatga
     2881 tcattaatac aggcgcgatc acgaataaac cgagccacaa aatataaggg acggaataga
     2941 tcctgcgcat tgttttcatt cttgtccccc ctattcttcg tagctgtcaa gacgtgcatc
     3001 gaattcttct tcagactcgt tgaaacgcat gacatggata tcttctggct caaaggccaa
     3061 tcctactttg cttccttctt tagctttttt cgttgaatgg accatccatt cattttgctg
     3121 ttcatcataa cagatgattt cataatggac accacgaaac agctgagtat cgaccgttac
     3181 gactagtttt cctttatctg gtgtcgtgat cgtcaaatcc tctgggcgta aaacaacttc
     3241 aactggttca tttagacgca tcccgccatc gacacattca aattgcttac caacaaactc
     3301 aactagatta tcttcgatca ttacaccgtc cacgatattg ctctccccaa caaaatcagc
     3361 aacgaaatga ttgattggct catcataaat atctacgggt gtaccacttt ggacgatttt
     3421 ccctttattc atgacaaaaa tctcatcact cattgccaac gcttcttctt ggtcatgagt
     3481 aacaaaaata aacgtgattc ctaatcgttg ttgcagatcc cgcagttcat actgcatatc
     3541 tgtacgtagt ttcaaatcaa gcgccgataa cggttcatct aatagcaata ctttcggttc
     3601 atttacaatt gctcgtgcaa tcgctacacg ctgtctttga ccaccagaca tttcactaat
     3661 ctcccgtttt tcgtatccag gaagctggac catacgcaaa gcatctttta ccttcttttc
     3721 gatatctgat ttggacattt tcttgatctt caatccaaaa gcaacgttat caaaaacatt
     3781 catatgagga aaaagtgcat aatcttggaa gactgtattt acttgtcgct tattagctgg
     3841 caaatcattc aaccgtttcc cttcaaaata gatgtcccct tctgtagctt cggtgaatcc
     3901 agctataata cgtaggatcg ttgttttacc acagccggaa ggaccaagca gtgtataaaa
     3961 ttttccttgt tcgatctcaa aacttacttt ttttaatact gtttcatcat catagcgttt
     4021 cactacttga tcaaatgaaa tgattggttt ccccacgttt ttacccccta tattacaaat
     4081 atgattgcgt tgcaacaatc aatacttttg ttcgtttttt gagatgattc cttaattgat
     4141 gttgttttgt cgcttgataa tagatcgact gtcctgcttt tgccacatag accgtctctc
     4201 ctaagcttaa ctctaattcc ccttctaata caaaaataaa cgtttcagat aatgaaggtt
     4261 caaacgtctt atattctcct gcaccttcaa aagtcagcaa gacaggctcc atttcttttt
     4321 cattcgaatc agccacgagc cattccaatt catacccgtt ttcttcatcg aaataaaccg
     4381 tatgatcttc ttttgaataa accacttgca gattcgctgc ttcttgatga aaaaattctt
     4441 ctggagtcac acctaagact tcaagaatag aaaagaatgt ttccattgat ggtgaactta
     4501 aatctcgttc caactgggaa atataccctt ttgacaaatc tgtgcgttcg cctagttctt
     4561 cttgtgtcaa attcttttgg atgcgcaaat ttcttaactt ctcaccaatt tccataattt
     4621 cctccgaatc gaagttttct aatagtaaac ttcctgttta ctaccactcg
//
LOCUS       DAHUWM010000193         4635 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHUWM010000193 DAHUWM010000000
VERSION     DAHUWM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.197"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            175..519
                     /locus_tag="NK788_002383"
     CDS             175..519
                     /locus_tag="NK788_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCH6770690.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="NK788_002384"
     CDS             520..939
                     /locus_tag="NK788_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCH6770691.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="NK788_002385"
     CDS             complement(1031..1381)
                     /locus_tag="NK788_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770692.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="NK788_002386"
     CDS             complement(1347..2567)
                     /locus_tag="NK788_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCH6770693.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="NK788_002387"
     CDS             2933..3499
                     /locus_tag="NK788_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6770694.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="NK788_002388"
     tRNA            3644..3731
                     /locus_tag="NK788_002388"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="NK788_002389"
     CDS             4065..>4635
                     /locus_tag="NK788_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCH6770695.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DAHUWM010000194         4593 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHUWM010000194 DAHUWM010000000
VERSION     DAHUWM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.198"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(130..1104)
                     /gene="lacD"
                     /locus_tag="NK788_002390"
     CDS             complement(130..1104)
                     /gene="lacD"
                     /locus_tag="NK788_002390"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCH6770696.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(1101..1559)
                     /locus_tag="NK788_002391"
     CDS             complement(1101..1559)
                     /locus_tag="NK788_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6770697.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1556..3019)
                     /locus_tag="NK788_002392"
     CDS             complement(1556..3019)
                     /locus_tag="NK788_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCH6770698.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(3001..3933)
                     /gene="pfkB"
                     /locus_tag="NK788_002393"
     CDS             complement(3001..3933)
                     /gene="pfkB"
                     /locus_tag="NK788_002393"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCH6770699.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            4075..>4593
                     /locus_tag="NK788_002394"
     CDS             4075..>4593
                     /locus_tag="NK788_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6770700.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGE"
BASE COUNT         1367 a         1028 c          732 g         1466 t
ORIGIN      
        1 taatgacaaa tgttattatt agttttagat aacttttgca gttttatact ttttttctat
       61 aaaatttgac aaacattttt ttaaataact aattattttt tgaatctgtc actcagacaa
      121 tcctttctct tacttgttaa cataaataga agttgctgtt tcttctaata gtgtacttaa
      181 ttctttaata ttttgttttc cttcttctaa catccaacta ctcgcttttt cttcgccttc
      241 ggtaacaaaa ggtgctactc ccttcgccca tgttgctcgc ccacataaga caccattgaa
      301 tgtcgaacct gattgttttg caaaacgcaa tgtatcttga aatagtgcag cgctgactcc
      361 cgcacttaaa aaaataaacg gttgttcagt cactttcgat tgttcataga aatacgcgga
      421 tgcctctgct tgactataac aagtatcttg cccatatcct tccacaaatc tcatattcac
      481 tggtacttct actttcaata catccacttg ataacgttct tttgaaaatt ctcgcatggc
      541 ttcaatcact ttatgcggtt ttacttttgc atagtcaaga gaagacggat cgccaatagt
      601 tgcatcgtaa gaaaggagct ctaaaaagaa aggtatgcct tctgcttcgc attctgaacc
      661 aatccgttct ataactattt gtttttgcac attaatctct actggttcat caacgtcata
      721 gtacaataaa aatttacaag catccgcacc cacttctttt aaccttttca ctgaccaact
      781 attcagtaaa tctggtagtc ggcctggcat tttcgcatca tagccggttt tttcataagc
      841 tagcaacaaa ccacattctt tctctcgaag cgctgctgca ggcagaccat attctggatc
      901 gagtaaaata gcggaagcat acggagtcag gtactgagaa accatctgtt taaattggat
      961 gatatcttct gatgtagctt ctttatattg gttgatcatt ttcttcaaag caccacgttg
     1021 gtcgatagct agcgcgctaa taattccagc ttcatccgtc aatcgcttca aacgttcctg
     1081 tttttctata ctcattttct tcatatatct tcacctattt ctttagttaa tattgtagct
     1141 atttctattt ttgattttgc agctctcaac tgttctgtaa cctcttttcg catcaatgat
     1201 cgtgcaacga gagacaataa tttcaaatga gtcgttcctg cttctgaatc tgggataaat
     1261 aacgcaaaaa taaaatcaac tgactgtcta tctaaactgt tccattcaat tccttttttc
     1321 agtgtgacga taatcaaact aggttcattg attgtttgag acttcgcatg agggatagca
     1381 aaacctgaca ttattcctgt agtaccttct ttttctcttt ccagaagttt ttgatatact
     1441 tctttttcgt catgagcgat tcctaaacag aatgtttgct ttgctaaaaa cttaaatgct
     1501 tcttcttgat tatctaatgg ctgttgtaaa aaaatgttct cttcatgaat gattgtcatg
     1561 ataaacctcc tgctcttcct tcttcgataa ctgtacgatt tgttgcttta ttatccctct
     1621 tcttgtcttt atcagttact cttttctttt ggatcaaacc tagtatcact ccaccaacga
     1681 tcgaaccaat aagaatcgct gtaacccatt ggatttttcc cgtcactaca ggtaacacta
     1741 aaaaaccgcc atgtggggct ggtacttgga caccaaacag ataggtcaag acagcagcaa
     1801 ttgatgagcc taacatcatc gttggtaata ctcggatcgg gtctttcgca gcaaacggaa
     1861 tagctccttc cgtaatatga gttgatccta aaataaaatt aaccaaacct gcattacgat
     1921 cattagtatc aaaatattta ccaaacaata aagtagcaaa accagtaatc aacggtggtg
     1981 caatgcaagc tgctgacgca ccagccataa aagtcgtgtt tccttgtgtc aaaagagctg
     2041 ttcctgttac atatgccgcc ttattaaccg gtcctcccat atcaaacgca cacatacagc
     2101 caacaatcac gccaagaaca agtggattcg aatattcaaa tccagctaaa aagctcatca
     2161 ttccttcatt tatagccttc attggacctg ccagcaaaga catcacgatg ccaataaatg
     2221 ctacaccaat aactggatat aagacgattg ccttcaagcc atctagttgt ttaggtaaaa
     2281 ttatcagtag cttttgcaaa aataagataa aataacctgc taagaaccca gctaaaatac
     2341 ctcctaaaaa tccagtaccg ccattcatgg ctacgattcc accaacaaat ccaacgacta
     2401 accctggacg tttcgcgatc ccttctgcaa tataagctgc taaaactgga accatcattc
     2461 ccattgctgc atcgtcaact gcttttacca ttgccgctgt agcattgtat tgtgcatgat
     2521 ctggatcaga agaatagatt ccccaaacgg caaatgaaat agcaatcaat actccgccag
     2581 ctaccacgaa aggcaacata tgagagacac cgttcatcaa atttttatag atgctacgac
     2641 caaaattagc accacttatc gcttctggtt ctaaagtatc agcagcttca ttatctgcta
     2701 agacagtacc ttttcctgcc aatgcttcgt caatcagccg ttgtgcatcc ttgattcctg
     2761 tactgacaga aacatcaatg actcgttttc ccacaaatct ttctgaatgg acatctttgt
     2821 ctgcggcaat aatgacagca tctgcctgtt tgatttcttc agccgttaat tcattctcga
     2881 tcccaatttg tccatgggtc tccactttga tcgtgatgcc ttttttccta gctgcttgct
     2941 ctaatgcctc ttgcgccatg taggtgtgcg caattcctgt cggacaaccg gttgctgcaa
     3001 ttagttgata ttttcccatc tttcgaacct ctcttccttg attttgattt gtttacttaa
     3061 ttcctccaca tccaaaaaat cagtcagtcc tttacgaaaa gcagtagagc tgcctgctgc
     3121 tatgctttta cgtaacgttt catcaagcga tcgtcgattc atgtatcctg ctaaaaaagt
     3181 gcctaacatg gcatcacctg cacatgctgt attgacaact tttccttttg gcgagtttcc
     3241 tcgaatcacc ctgtctttcg taaaaagaat cgctcctttc tcaccgaggg ataacaaaac
     3301 attttctgcg cctgactcta ctagtcgttg tccataaaca aagtaatctt catctgtaac
     3361 catttttcgt ccgaaccaag aagaaagttc ttcctcattt ggtttcagca aaaacggtcg
     3421 atagggtaaa caatccatga cttcttgatc actactatca ataatcaaac aaatccgact
     3481 gatttctatt aaaacctttg gagacagccc tcttggtaaa ctcccagaaa cacaaagata
     3541 atcccctgct tgtaatgctc ttatttgatt taaaaaatta tgaacagctg tctgcggtat
     3601 ttcaggtcct tgattcacta atttgtattc ttctcccgtt tgattgactt gtgtaaatac
     3661 attgatacgc gtcataccag gcacttcaat aaactctgtt tggatttgtt tctgctgtaa
     3721 atagtcttct atatactttc ctgtgaaccc agcagaaaaa cctaatgccg tacttgggaa
     3781 atctagcatc tttaatacta gtgacacatt cacccctttt ccgtttgcct gaatgtcatc
     3841 ctcttttgtg cggtttacca taaaaggatg catctcatct gtttcaataa atagatcaat
     3901 tgccaaattc atcgtgcatg tgtagataga cataaccaac gacctccttg attctattat
     3961 gaaatacgcg aaatagaaaa tgttttctta atcgtcccaa cacaaagaaa acctttacat
     4021 ttataaactc gtcgtgcttt ttttaactaa cttttggtga aagaaggaaa gaatatgaat
     4081 cacaaattaa gtgaaacaga gcagtacctc tggaacttta tcgaacacct catcctagag
     4141 atccctaact attccatcgt gaaactaagt gaacaagcaa atgtctcaac agccaccatt
     4201 gtgcggacaa tgaaaaagaa aggctatgaa gggtttactt cgtttaagca tcatctaaaa
     4261 gaaggagaaa acaagaacat caatttttca tttttagata aagtagacaa aggaatccgc
     4321 cgagccattc taaaaaatga acaagaagtg gtacgtacaa tcaatatgtt ggaaatcggg
     4381 aacattgaag atgcaataca aagaatcaaa tttgctgacc gggtttttgt ctttgcccga
     4441 ggattttcag aaatgattgg ccaagaaatg ctggtaaaac ttcaattaac gggaaaaaat
     4501 tgtcagatgc atactgatcc agaaattatc aaaagcatca gtcggaaact aactaagaaa
     4561 gacagcgtta tttttgtttc tttgaacggt gag
//
LOCUS       DAHUWM010000195         4547 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHUWM010000195 DAHUWM010000000
VERSION     DAHUWM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.199"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..954)
                     /locus_tag="NK788_002395"
     CDS             complement(<1..954)
                     /locus_tag="NK788_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6770701.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGG"
     gene            complement(956..2395)
                     /locus_tag="NK788_002396"
     CDS             complement(956..2395)
                     /locus_tag="NK788_002396"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCH6770702.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2580..4526
                     /locus_tag="NK788_002397"
     CDS             2580..4526
                     /locus_tag="NK788_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCH6770703.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1436 a          783 c          757 g         1571 t
ORIGIN      
        1 tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt cgcctttaat
       61 ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt gtcttattgt
      121 tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa taatttttaa
      181 atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa tggctgttaa
      241 atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact ttatttccat
      301 ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat ttggattttg
      361 aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat ttcctgtaat
      421 cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt ttcccccttc
      481 aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa aagcaacaat
      541 aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg agattatgcc
      601 atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag gatcattttg
      661 actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga gatactcaat
      721 gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa gctgaggtga
      781 ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt tcatagcttc
      841 atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga cgcgtgaaac
      901 ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta ccattttaat
      961 attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata ccattttggt
     1021 gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata aaaatctcaa
     1081 tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt gagcattcac
     1141 gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct aaaataattt
     1201 ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca gagaaaagaa
     1261 gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta ttatttgttt
     1321 ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct tctactggat
     1381 attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt gctccctgat
     1441 aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg acaatcagag
     1501 cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca taatcaagat
     1561 tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat tgacagatct
     1621 taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac ccttgaggac
     1681 aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca atcatgtatt
     1741 ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc acgttattgt
     1801 catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct ccaacaatcg
     1861 catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca gttacatcag
     1921 caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta tccatccatg
     1981 ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct gtatagagta
     2041 agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg ctatctcgtt
     2101 ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca tctgattcat
     2161 tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga taaaaaagcg
     2221 tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct gttttaggtt
     2281 cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt ttatccatct
     2341 catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag ttcatatttt
     2401 actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg aaaaatcaat
     2461 catttcattt aaaaaaataa actatatgac aaacgtttga catattttta agatatgcta
     2521 aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga gtgattcgaa
     2581 tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat aaccttattg
     2641 cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg aaagtgaatc
     2701 aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat ggacaatatc
     2761 aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc aaaaaaactg
     2821 gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat aaaaaattca
     2881 accccattat ggcattaatt aaattattat cagatatttt tgttccaatt atacctgccc
     2941 ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct ggactttttg
     3001 gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca atgatccaac
     3061 tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca gctgctaaac
     3121 gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca acaccagatc
     3181 ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa acaaactatg
     3241 cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta gaaaaattct
     3301 ttcataaaaa attgccatca tctattgact ttacattcac gccattatta tcagtaatga
     3361 ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg tctaatggaa
     3421 ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg ggtatttttg
     3481 gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca gctattgaaa
     3541 ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc tttgtagttg
     3601 catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc ttaactaaaa
     3661 attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt ggaattacag
     3721 agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt gctttaattg
     3781 gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt tcacttggtt
     3841 cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt tacgtattgt
     3901 gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt aaaacaaaag
     3961 ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat tcttcaaaag
     4021 tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa gtttcatcag
     4081 aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta aatgatccag
     4141 tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga aacacggtcc
     4201 attctccagt agatggtact attcaactca cgtttgaaac tggacatgct tatggtatta
     4261 aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta tcaatgggag
     4321 gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc gatattcttg
     4381 gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca atgattattg
     4441 tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca gtcactgagg
     4501 gtactgattt acttgaaatc aaataaatat atttccctga gtaaaga
//
LOCUS       DAHUWM010000196         4502 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHUWM010000196 DAHUWM010000000
VERSION     DAHUWM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.200"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(5..667)
                     /locus_tag="NK788_002398"
     CDS             complement(5..667)
                     /locus_tag="NK788_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6770704.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            851..2497
                     /locus_tag="NK788_002399"
     CDS             851..2497
                     /locus_tag="NK788_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCH6770705.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2488..3570
                     /gene="uxuA"
                     /locus_tag="NK788_002400"
     CDS             2488..3570
                     /gene="uxuA"
                     /locus_tag="NK788_002400"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCH6770706.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3678..>4502)
                     /locus_tag="NK788_002401"
     CDS             complement(3678..>4502)
                     /locus_tag="NK788_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCH6770707.1"
                     /translation="YEYIDFFIDLSVDILRPNLPICREQRILVNILARLSITKIESEE
                     TSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLN
                     FNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDL
                     VHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISD
                     CPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         1433 a          784 c          887 g         1398 t
ORIGIN      
        1 aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca
       61 tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag
      121 ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca
      181 ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt
      241 cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag
      301 cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta
      361 ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt
      421 ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac
      481 tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg
      541 gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt
      601 ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc
      661 ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga
      721 aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg
      781 acatagcgat ttttattgca taaaattcac ttgaatacaa aaactaatat gttagtttag
      841 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
      901 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
      961 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
     1021 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
     1081 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
     1141 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
     1201 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
     1261 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
     1321 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
     1381 tattcttgct gtgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
     1441 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
     1501 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
     1561 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
     1621 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
     1681 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
     1741 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
     1801 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
     1861 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
     1921 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
     1981 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
     2041 tccaaatatt cctaatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
     2101 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
     2161 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
     2221 aggaaaacca ttcatcccga gtccagaccc actattgaca gaccttcagc ataagctttc
     2281 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
     2341 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
     2401 agaaatgttc atcaagatgg tagcaggacc aaatgccgta cgaaaaacat tacaggatta
     2461 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
     2521 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
     2581 acattgatgg atataccagt aggagaggta tggcctaggg agaggatcaa agaactaaaa
     2641 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
     2701 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
     2761 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
     2821 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
     2881 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
     2941 gaatttggat tgccgggatg ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa
     3001 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
     3061 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
     3121 tactcgattt ttggattagc tcgaatcgta aagaatcgag aagatttaga ttggttgtgt
     3181 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
     3241 ccagaaaatg atgtatatgg gattttagca gaattttgcc aaagagatcg aatcccattt
     3301 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accacataaa
     3361 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt
     3421 aacggataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
     3481 tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc
     3541 gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat
     3601 aagaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt
     3661 tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt
     3721 gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct
     3781 tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc
     3841 aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc
     3901 gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca
     3961 cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt
     4021 actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt
     4081 tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaat
     4141 acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta
     4201 agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt
     4261 tcaggtataa acaaatcaaa tacagtacaa agggactcca ccatttctct tgaataaaga
     4321 acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat
     4381 tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa
     4441 attggcagat taggtcgcaa aatatctaca gagagatcaa taaaaaagtc aatatattca
     4501 ta
//
LOCUS       DAHUWM010000197         4499 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHUWM010000197 DAHUWM010000000
VERSION     DAHUWM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.201"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(48..659)
                     /locus_tag="NK788_002402"
     CDS             complement(48..659)
                     /locus_tag="NK788_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6770708.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(782..907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(989..1705)
                     /locus_tag="NK788_002403"
     CDS             complement(989..1705)
                     /locus_tag="NK788_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCH6770709.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="NK788_002404"
     CDS             complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="NK788_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCH6770710.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2264..3061)
                     /locus_tag="NK788_002405"
     CDS             complement(2264..3061)
                     /locus_tag="NK788_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCH6770711.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3031..3402)
                     /locus_tag="NK788_002406"
     CDS             complement(3031..3402)
                     /locus_tag="NK788_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCH6770712.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="NK788_002407"
     CDS             complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="NK788_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCH6770713.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1222 a          981 c          786 g         1510 t
ORIGIN      
        1 ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct
       61 gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc
      121 agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca
      181 atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac
      241 attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg
      301 atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta
      361 aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct
      421 accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct
      481 gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta
      541 aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact
      601 aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt
      661 tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa
      721 aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa
      781 gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt
      841 tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa
      901 gacgaacctt atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca
      961 acatctaaca attataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt
     1021 cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt
     1081 atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt
     1141 gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca
     1201 ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg
     1261 tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat
     1321 ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca
     1381 agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg
     1441 gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt
     1501 taggtttgta caataaatag taaccgtatt cttcttgata aattgggact tcatccactt
     1561 ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt
     1621 ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg
     1681 caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa
     1741 tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc
     1801 ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg
     1861 ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg
     1921 taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg
     1981 tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc
     2041 ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct
     2101 ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc
     2161 tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt
     2221 actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat
     2281 gttcggtatc tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg
     2341 caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac
     2401 ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac
     2461 gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt
     2521 ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga
     2581 tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca
     2641 taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg
     2701 catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt
     2761 cccataattc ttcatcgtct gtgccgaatt cttggacagg aagcaatgtc ttgcttttga
     2821 ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt
     2881 tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata
     2941 tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat
     3001 ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca
     3061 ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc
     3121 ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga
     3181 aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat
     3241 gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt
     3301 atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc
     3361 catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga
     3421 ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt
     3481 ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc
     3541 agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc
     3601 tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga
     3661 aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa
     3721 tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat
     3781 gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc
     3841 tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa
     3901 gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc
     3961 tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg
     4021 ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag
     4081 acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag
     4141 aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag
     4201 aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc
     4261 ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct
     4321 catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag
     4381 caaagaaaaa gccgtctaga cactcagacg gactggtttc ttttttcggc tctataattt
     4441 atgggactga tgaatcagaa ttgattcttc agccccattt tcattttagg tattaaaaa
//
LOCUS       DAHUWM010000198         4492 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHUWM010000198 DAHUWM010000000
VERSION     DAHUWM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.202"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            46..1443
                     /locus_tag="NK788_002408"
     CDS             46..1443
                     /locus_tag="NK788_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6770714.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            1524..1964
                     /locus_tag="NK788_002409"
     CDS             1524..1964
                     /locus_tag="NK788_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6770715.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            2112..2438
                     /locus_tag="NK788_002410"
     CDS             2112..2438
                     /locus_tag="NK788_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6770716.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            2439..3188
                     /locus_tag="NK788_002411"
     CDS             2439..3188
                     /locus_tag="NK788_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770717.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(3268..3723)
                     /locus_tag="NK788_002412"
     CDS             complement(3268..3723)
                     /locus_tag="NK788_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCH6770718.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(4150..4425)
                     /locus_tag="NK788_002413"
     CDS             complement(4150..4425)
                     /locus_tag="NK788_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770719.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         1486 a          722 c          896 g         1388 t
ORIGIN      
        1 ttcgttatga ttacggtatc aagaaaaaag agaggaagta aaaaaatgaa aacctacaca
       61 ttcccagaaa atttttggtg gggttctgct gctagcggac cgcaaacaga aggaagagta
      121 agtgatgatg gtaaaggaga aaatatttgg gatcattggt atgaattaga gcctgaaaaa
      181 ttcttcgatc aagtaggacc tgaaaaaact tcgcaagttt acacaaaata caaagaagat
      241 gttcagttaa tgaaagaaac tggccataac acatttcgta catctatcca atggagtcat
      301 ttgattccag aagggacagg tgaaatcaat caaaaagctg tttcttttta tcgagattat
      361 tttactgaat tgctggataa tggtattgaa ccttttgtga acctttacca tttcgatatg
      421 ccactaccac tgcaagaaaa aggcggatgg ttatcaagag aaacggtcga cgcttatgtt
      481 tcttatgcag cagcgtgctt tgaactgttt ggagatctgg tcaaaaaatg gtttactcat
      541 aatgaaccaa tcgtacctgt agaaggaggc tacttgtatc aattccacta tccaaatcat
      601 atcaatatga aagaagcggt ccaagtaggc tatcatgaga atctagcaag cgcaaaggta
      661 atcaaagcct accatgaagg cggacatgat ggtgagatcg gaatcatctt gaatctgaca
      721 ccaagctatc cgagagatga aacgaaccca gaagatgtaa aagcggcgaa gatcgctgac
      781 gcattcttca accgatcatt ccttgaccct gcagtgaaag gagagtttcc acaagagttg
      841 atcgaaattg tcaaagaatt ggctataatg ccagagatcc atgaaggaga cctagaactc
      901 atccgtgaaa acacgattga cctgctagga atcaattact atcagcctcg tcgaatcaaa
      961 gcaaaagaaa ctccaatcaa tcacgaacat agccccatgc cagatgatta ttttgataat
     1021 tacgacatgc ccaataaaaa aatgaatcca tatcgtggat gggaaattta cgaaaaagga
     1081 atctatgata ttttaacgaa tacaagagaa aactatggta atatcaaatg ctatatttct
     1141 gaaaatggga tgggggtcga aggagaagaa cgatttgtta acagcgaagg aattattgaa
     1201 gatgattacc ggatcgagtt tgttacggat catttgaaat acgtccatca agcaatccaa
     1261 gaaggaacca attgtgtggg ttaccacatg tggacatgta tggacaactg gtcttggaca
     1321 aatgcttata agaatcgcta tggctttatc gctgttgatt tggcacaaga cggtaaacgg
     1381 acgatcaaaa aatcaggtcg ctggtttaaa acggtctctg aaaaaaacgg atttgatgcc
     1441 tgatattttt tgataaaacg ttcacaaaat gtttgttcta acactgtaat cgtgctacac
     1501 taacagtgaa ggaggcgttt acaatggtag atacttatcg taatatttta gtagcaattg
     1561 atggttctga aaaggcagaa aaagcatttc ttgaggcaat cacgattgcc aaacgaaatc
     1621 aggcaacgtt gcacatcctg tatgtcaatg aagtaacagg caattacttt ggtgattttg
     1681 catttgttac aacaagcctt caggaagaat tggatgaggt tgcggaaaac cagatgaaag
     1741 aacatcgaaa cttggcaatt gaaaaaggcc tgacagatat cgagacatat gttctttacg
     1801 gttatccaaa gacactgatt gctaatttcc atgaatcgaa agaaccaatc gatctgattg
     1861 tcatgggggc gacaggtctg aatgcggtag aacgtgcact agtaggttcc actacttcct
     1921 atgtagtcaa ccacgcatca tgtaacgtca tggttgtaaa ataaataaat taagggaaca
     1981 gaaagcaaaa aagcataacg cttcttttgc tttctgttcc ttttattttt ttgtaaaaaa
     2041 ttactttacc tgatgaagta atcatgctat tctaattaca acgacagatg gagtaatatg
     2101 gaggaaagaa aatgatttca agtgatggaa tacgcggata caatgatgcg attattttat
     2161 cgatccttaa caaaaacgat tcttatggat atgaaatttc taaaacgatt cgtgagagga
     2221 caaatgattc ctatgtgatc aaagagacaa cactttactc cgtttttgcc cgtttagaaa
     2281 agaatcaact gattgtttcg tatcctggag aagtgactca tggaaaaaag cggacatatt
     2341 ataaaatcac tgaaataggg cgtaatcttt tagaagaaaa aaagcaagag tggctgacga
     2401 cgaaagacgt agtcgatcaa tttttggagg gaaattaaat ggatacaatc cgtaattatt
     2461 tagacagtct ttttatcgga gtacctcaaa gtaccgagat agataagctg aaaactgatt
     2521 tgttggcaaa tatggaagac cattatcatg aattgatggg agaaggaaaa aatgaacagg
     2581 aggccatcgg tacagtaatt tcgacatttg gttcaatcga tgaattatta gaagaactag
     2641 atgtcgaaaa aaaacatcaa gctgacgaga cggaaacaaa cacagcttct atttatttaa
     2701 gcgaagcaga aaactactgg aaagaataca gagcggcaag tctccaagtg gcatcaggtg
     2761 ttctattcat tagcttatct tttgcaagtt ttcttttctt ttgttcagca ggatatgtgt
     2821 tcatgggtat ttcttgtctg attttcggta tagcattagc tgtcggtttt tttattgctt
     2881 ctggtatgaa aatcacacgt ttaaatcact ttttgcacca tcggaaaata cctgaaaaag
     2941 tattagcaga agcaaaagaa aaagaagaag agtatcagcg aagttttggc ttctcactga
     3001 ttgcaggaat tggattatgt attttttcac ttttcccact tttggcttct ttaatgtggt
     3061 atatggatgg tagtatcggt gcctctatat tttttgttac ggtaggaacg ggtgtcttct
     3121 tgatcattta tggcagtcta gtccgtcatt cttatcgtca attcactcaa agcgcttatt
     3181 actggtgaat gtattcagat ttgtaaaaat attctattta tccggcgtag aaaaaaagac
     3241 tgtgacacgc ttttgtcaca gtctttttta tatataataa tttaaaaatt catcatctaa
     3301 atccgtagaa ttttctagat tgcttaccca tgtaaaatcg ttatagacag atttcagttg
     3361 acttctgcgc cattgtacag ggataaaaag acgcatgcta tcttcattga atccgactaa
     3421 taatttcttt ccgtcagtaa tgatgggacg ttgtaacaaa gtaaaatgcg agacgatcag
     3481 atcgacaagc tctgaggtcg tacattggtc gatttggtct ttcaaatttt tataatcaat
     3541 cgagcgttta gctaatagat cgtcaaatcc gttttctgtt aatgacaata aataaaatat
     3601 ctctttcctt gttaaaggct gtttaggtgt gaaaattcgt tctctgtaag gcatttgatg
     3661 gtctttcagc cagtttgcca tcagatggta actatgtgtg cgagaagaat agatcgtaag
     3721 cataaaaaaa ctccttaaat agtccattaa tttgttagat ctgttgagat aaataatagg
     3781 taaggagctg tgcataagca ttgatcaaaa caacccaaac atcattagaa ataactttct
     3841 aaagtcttaa acttgcgacg agaaacaact agactaatag aaaaaatgtc ttagccattt
     3901 ggcttcctgt ttgttttttt gcttatgctg cagctcctcg ggaaagggtt ctaacaattt
     3961 gaagaaggtt gatgattgat ctagtttaaa ttgtgtgaaa tttgaaatct ggtgttttta
     4021 ctagatgaat cacctaacaa ctatgattat aacttataga acttctgttt tgcattcggt
     4081 tttgttatag ttgttataaa gttttatttt attacagaac atttttaatt aataaagaac
     4141 agttagaagt taaaattgct gattcacata gagtttatga cttcttggaa attctttttc
     4201 aatcagtttt ttcatctgtt tatgaaattc ttttttttca gtaaacataa aggtttgggt
     4261 atcgtaacct tcttcatgta ggaaatagat tattcctttt tctgtgtttt gcgtataagc
     4321 ttttatactc ctgtcttttt tgaaagaaca gatcgtatag ccatctattt ggtctggtag
     4381 tcgtttgatt ttcttcaaaa tgactttttt atcctcaagt ttcatttttt tcacctgact
     4441 ttattttttt catatggaaa tcatagcgcc tttggttata tgaaaacaag aa
//
LOCUS       DAHUWM010000199         4468 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHUWM010000199 DAHUWM010000000
VERSION     DAHUWM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.203"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            316..1047
                     /locus_tag="NK788_002414"
     CDS             316..1047
                     /locus_tag="NK788_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCH6770720.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1192..3219
                     /locus_tag="NK788_002415"
     CDS             1192..3219
                     /locus_tag="NK788_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCH6770721.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3327..4424
                     /locus_tag="NK788_002416"
     CDS             3327..4424
                     /locus_tag="NK788_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCH6770722.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1568 a          717 c          923 g         1260 t
ORIGIN      
        1 ataatataca ttttttattt aattataatt aatattgatt gaagtctagg cagtcaagtc
       61 acattttgat agataggagg gggacttacg aattatttta aaggctatga cagctgtatt
      121 tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg gctgataatc
      181 atttataact aatcgataca taggaccaaa tataggtcta tgttctttta tttcataagt
      241 atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt agtaaataaa
      301 gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat gatgttattc
      361 agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa ggaatataac
      421 acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc tgttgatcca
      481 gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc tggaacagga
      541 ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa aatttctact
      601 gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag aaaaccgaca
      661 tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag tgtctctcaa
      721 ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct gaaatttacg
      781 aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt gaattctgaa
      841 ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc aggaacaggt
      901 gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga tgcggtacag
      961 ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa attagtttgg
     1021 acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa ataaatacgt
     1081 agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc taaatttgtt
     1141 tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat attgaaaaag
     1201 aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc gatcataggg
     1261 tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga aagcaaactc
     1321 acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag tgaggattta
     1381 agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc ggacaaacaa
     1441 ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa tattatttct
     1501 atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga aaatgggaag
     1561 ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc aaataagaag
     1621 attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa tcaacgagat
     1681 agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc tagtaatccc
     1741 agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg ttctggccaa
     1801 ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag tggttcttct
     1861 gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt cgcttttgtc
     1921 acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac ggtagctcct
     1981 acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc ggagcaaaga
     2041 agtcttactt atgatttgga aggtgtcggt cactcttctc ggattatacc tgatctcatt
     2101 aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg tacttttttt
     2161 caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac agctgatgta
     2221 aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga taagataact
     2281 atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat gcagactagt
     2341 atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg aaatcaaatt
     2401 gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac agattatttt
     2461 attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt aattcctgaa
     2521 aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga gcaatcgaat
     2581 gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta ttataaagat
     2641 aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat aggagagaag
     2701 tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc agcgaataca
     2761 gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa aggcaaactt
     2821 tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc cagtagacca
     2881 ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg ggcttttgga
     2941 agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt tgagcttcca
     3001 gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc cgctgttacg
     3061 attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga ttctacagct
     3121 catcgtggaa tcagaataga tattcctgta gaaaaccaaa gaatcggaac ctttgagggg
     3181 aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa gaatataaca
     3241 aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc acagagacaa
     3301 gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg agaccaaaac
     3361 aacaattatt tataacactc ctagttattt taggggcact ttttaacaat atatcagtgt
     3421 cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa atcgacaaga
     3481 gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta aaagcggaat
     3541 tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc gcaaccacca
     3601 attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg agcctgatct
     3661 ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct aaaagtgtcc
     3721 aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta ttggctggtg
     3781 ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag gatcaaagct
     3841 tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag acactgaacg
     3901 aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat gcaagaaatg
     3961 tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta gttattgaaa
     4021 caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa gaaggattgc
     4081 agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa ccgctaaccc
     4141 caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat ggaaaatttt
     4201 cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt gaaaaagatt
     4261 ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg attaaaaaag
     4321 acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt ttattatgca
     4381 tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa gataacaaat
     4441 agtaaatttt tattattgta ggatggtt
//
LOCUS       DAHUWM010000200         4440 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHUWM010000200 DAHUWM010000000
VERSION     DAHUWM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.204"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            529..1440
                     /locus_tag="NK788_002417"
     CDS             529..1440
                     /locus_tag="NK788_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCH6770723.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1927..2193
                     /locus_tag="NK788_002418"
     CDS             1927..2193
                     /locus_tag="NK788_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770724.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2265..2606
                     /locus_tag="NK788_002419"
     CDS             2265..2606
                     /locus_tag="NK788_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770725.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2661..3080
                     /gene="ssb"
                     /locus_tag="NK788_002420"
     CDS             2661..3080
                     /gene="ssb"
                     /locus_tag="NK788_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCH6770726.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3157..3453
                     /locus_tag="NK788_002421"
     CDS             3157..3453
                     /locus_tag="NK788_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770727.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3468..3899
                     /locus_tag="NK788_002422"
     CDS             3468..3899
                     /locus_tag="NK788_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCH6770728.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4081..4386
                     /locus_tag="NK788_002423"
     CDS             4081..4386
                     /locus_tag="NK788_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770729.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1632 a          699 c          950 g         1159 t
ORIGIN      
        1 caaaaaccta agcgggaagt ctccgaataa cataccaagc gccgacagcc aacagataaa
       61 gaatacacta ctaaaaatag tagtagcata aaatttaaaa gggaaaaaat caacccctac
      121 cccagtcttg gggtctcaaa actaactccc ccctaacccc ctctttcagc ctcttttgaa
      181 agagggggag aatcaacccc acggccaacg ccccagtgtt cttggggctg tccagaacag
      241 aaacttgcga gagagtgggg gtaaaacaag tgtagaggaa acttgaagac ttgttcgaac
      301 agaaacttgc gagagagccg gagtaaaaca agtgtagagg aaacttgaag acttgttcga
      361 acagaaactt gcgagagagt tggagtaaaa caagtgtaga ggaaacttga agacttgttc
      421 gaacacaaac ttacaagaga ggggagtaaa gcagttgcag aggaatcagg aagatttgtt
      481 cgaacagaaa cttgcaagaa agcattatta aattgaaggt gatgaaatat gaagtattta
      541 ttgcatggct attttattga ttattctact cgcaatagta gtactttagc gattctatgt
      601 ttgcttcatg aattgagagt ggttactaga aaacagctat acaaactatt gaattttgac
      661 agatcaatta gtaaacaagg agtaacgaat gctttagtga gattatatga aaaacagtta
      721 atcgatcgaa aaaaatatgg aacagaaatg tattattatt tgacgaaaga gggacatcaa
      781 agcataggtg ggtattatac gttgccgaaa gttcctgaat ataatttgaa tcaccattta
      841 caaattaaca attatttgat taaaggaatg gcattggcaa aagaccaccc acatttaaaa
      901 cggattattt cagaaagacg taccgtctat gaaacgaagg atttaaatcc taagacaaaa
      961 ggaagaaaat tttttgtgcc agattttatt tttcgttttc gagacaaaga aaatcatgag
     1021 atcaattggt cctttgaaat tgagctaacg ttgaaaacac gtcgtcgcta ccgtgaagcg
     1081 atttttccga aatatattcg tgagttgcga acacgtcctg aatcacattt aatttatgtg
     1141 acacctagtg gcgcaattca agaagaactt catgcgttta agcgtatttt tttcaagcgc
     1201 gaaactgaaa aagatgcgag tcaacaagaa ttgtttgagc ggttgcatat tttttcagct
     1261 ttagagtttg aagagggatt aaaagcatta ttagagaatg atccagcagt caattggcgg
     1321 acagtagaac gagaaaaaat ttatccgaat ggcgctgttc aaagtcgttt gaatttattt
     1381 gaagaatcaa ctgaaaatga ggaaaaacca aaagtgaagt ttacgcctga agtattgtaa
     1441 aagagggtgt tcgcttcgct ctttttttgt gccagtttgc tggcaccgtt cagacgcaag
     1501 cgtctgaacc ggctcgtttt taggaaggac taacttgttt gatttatccg ttgtcaaaga
     1561 agtggacgtt cgagagtccc taatcgtata gcagtcattc ggcgagtata acctaaaaca
     1621 taacctgcca attgccaagg gtaaaatgca cgctgcgcgc ccttggcaat cgtcagaaca
     1681 tgttttcgag ccggttatgc cgaacgaccg ctatacgaaa agggcctcta aacagaagtc
     1741 cactttgatc cttgacaact gaaacccaaa atcaaacaag caggccttcg tctaaaaacg
     1801 agccgaatga agaccttggg ggttgcaccc ccaaaccccc gtgcccccga cacactaccg
     1861 aaaaggtggg ggtgggtggc gaaaaaatca aataaaacat tctattcaag aaaggaagaa
     1921 acaaccatga caactaaaaa cgagcaactg aaagaacagg caagaaaaga gttgcaagaa
     1981 agaggtttga ttattgaggg gagctttgaa ggagattttg aaacctatat tggttgttat
     2041 gcaagaccga taaacaaacc tacagcctta gacccaatga acgaacaaga agtaaaagaa
     2101 caagaaaaac atgcagtgaa tggttttccg caagatttca ctgaatggta tgagtgggaa
     2161 attaacaacg ggaagcttga aaacttctta taaaagtcag atgacgtgca aagagaatga
     2221 ttctctttgt acggtaaaaa tataaagaaa gaaggaaaaa gaaaatggca aaaaacacac
     2281 cttatcaagt aggagacatt ttttatagta gttggggcta cgaacaaacg aacgtgactt
     2341 tttggcaaat tgtgaagtta acagaaaaaa cagcatggtt tcagcctatt caaaagaaaa
     2401 cggtcgagca ttattcgtcc atgagtggaa aagttgttcc aatgaaagac aagtttatag
     2461 agtatccttt agcaaaaact aaagacagta ttgtaaaaaa acgaattgat ccagaaacct
     2521 atcctgatat gttatgtggt tatcgtagtt gggacacgtt tcatagatat aaaaatcaag
     2581 ctgtttatga aagtagttat tattaagggg atagtttccc ctttcaaaaa taataaatag
     2641 aagaatggag agaaaaaatc atgatgaatt cagtgaattt agtaggacgg ttaacaaaag
     2701 aagtggattt aaaatacaca ccaaaaggca atgcgacagg cacatttatt ttagctgtta
     2761 atcgaaatta tacgaatgca aacggcgagc gtgaagcaga ttatatccgc tgtgtcattt
     2821 ggcggaaagc tgcggaaact ttagcaaaat atactggaaa aggaagtttg attggcatta
     2881 atggacgtct tcaaacgagg agctatcaga atcagcaagg acagaccatg tatgttacgg
     2941 aagttctagt gacagatttc tatttgttag agagtaaaga aatcaatgag caacggtcaa
     3001 aatcaatggc gaataatcaa aataattctt caattccagg aaatgagatt caaatttcag
     3061 atagttattt gcccttttaa gtgaaaaata attttgttct atacctgatt tcttcaggta
     3121 tagaacaaaa aataaataga gatgaggcga ttttttatga aaatcattga cagttacgat
     3181 caacgaacca tgcaatattt gggctcgttt gattatacag atgaaaatat tattaattat
     3241 gttgcaagtt tacctcctta tcaaagcgtt cgcttggtag attttgcaag tgatgaattg
     3301 gtattaacaa cgatgggcaa ctttttagat tatgtcggag acaaaaaatg gttggaaaaa
     3361 attttgtcta aattgattgc gaaacaaaca ggagaagaac ccataaatgc gattcaattg
     3421 ttcaatcgat atgaacaaga aaacaatgat taaagaggag agaaaaaatg aaaagattaa
     3481 aacgaagtat ttttattgtg ctagcattga ttgattttgt tgggacaggt tgtgaagcaa
     3541 ttgacgttca aaaagctccc ttatcggaaa taaaacaagc aataacaaaa caagatgaag
     3601 aacaaccgct aaatacacaa gtagaaatag tgaatcaaaa taaaccgagt ttttctgaag
     3661 aagatttttc gttagaaaat ggaaccaggc aatttttttc agatttggat cgtttaaatc
     3721 gggtaggtgt agcaagtgca cttttgcata aaagcaggat gccaacagaa gaacgagacc
     3781 gattatacat taagccaact ggctggaaac aaaagaaaat gaagaatggc gattatcttt
     3841 ataatcgttg tcatttgatt ggctatcaac gtgcgacaag tttccccata agtatgtaat
     3901 acgaaacggg aggattaatc ttaagattaa tcacaactaa cctatttgac agttggaatg
     3961 aaagctatca tgtctgctga atcaactggt ctgatactcc tacatgcaat aaagtctact
     4021 aggaagttat tgtatgaagc taggtaaagg cggcagaaca atttctaagt gtggaatcat
     4081 atgaagttgg atatgccggg acgctataaa ttactcaggg tgagactgtt tttaattaaa
     4141 ctgacgaact tgcgaatgta cgggtctata accgacttat attcgaaaga tataagattg
     4201 cgaatgcaaa gaacattatc gaaaagtaag aatgttggtc atgaaattcg agatggaaga
     4261 ctagcggtgt atctgtcaag agttggtaaa acatgtttga ttgattcaga tgagagtggg
     4321 aatgcgacaa aacgttttac agaagaaata gagcaactag cagaactgaa tatacgatat
     4381 ttttaaatac tttagcggag aataaaaaaa tcctctacag aaaactacgg agggcaacat
//
LOCUS       DAHUWM010000201         4425 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHUWM010000201 DAHUWM010000000
VERSION     DAHUWM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.205"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            690..1658
                     /locus_tag="NK788_002424"
     CDS             690..1658
                     /locus_tag="NK788_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6770730.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="NK788_002425"
     CDS             1669..2484
                     /locus_tag="NK788_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCH6770731.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="NK788_002426"
     CDS             2552..3262
                     /locus_tag="NK788_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6770732.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="NK788_002427"
     CDS             3298..>4425
                     /locus_tag="NK788_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCH6770733.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAHUWM010000202         4345 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHUWM010000202 DAHUWM010000000
VERSION     DAHUWM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.206"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(387..1853)
                     /locus_tag="NK788_002428"
     CDS             complement(387..1853)
                     /locus_tag="NK788_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6770734.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="NK788_002429"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="NK788_002429"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCH6770735.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DAHUWM010000203         4331 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHUWM010000203 DAHUWM010000000
VERSION     DAHUWM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.207"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..1381)
                     /locus_tag="NK788_002430"
     CDS             complement(<1..1381)
                     /locus_tag="NK788_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCH6770736.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYW"
     gene            complement(1350..3503)
                     /locus_tag="NK788_002431"
     CDS             complement(1350..3503)
                     /locus_tag="NK788_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCH6770737.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            3761..3997
                     /locus_tag="NK788_002432"
     CDS             3761..3997
                     /locus_tag="NK788_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCH6770738.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            4016..4231
                     /locus_tag="NK788_002433"
     CDS             4016..4231
                     /locus_tag="NK788_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCH6770739.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         1355 a          757 c          691 g         1528 t
ORIGIN      
        1 gccaatatgt tttttctcct acaattaaag acaactctcc agttaatcca taacgaaacg
       61 gaaacgattg agttgttttt aacgttcctt tcactgcata aaaaacacct ttctcagtgg
      121 cagtagaatt ttctgaaatt tcatctaaag ttccgtcgat cgtaatcgga gccccacctt
      181 tttgatccaa tttaaaatga accttcatcc cttctttaat ataggtactt tcattcgcag
      241 gtaaaagtgc tgtaaatttc atttttgatt tttcgtggat cggataaatt tctgcaagca
      301 ctgttccttt ggctagttct gcttgtccct ttgtttcttc atttaaatga accgttccag
      361 atacaggagc ttttagtatt ccttttgaaa gttgatcgtc caaacttttg ataactgact
      421 ggttcttctc ttgtttttca tttagctctg taactgtttg ttttacagtt gttattgctt
      481 gttctagctc ttgctttcct ttttccgttt gactaattct gtcattctca gaagtagttg
      541 gtttaactaa tttagctttt tctgactgat actgttctac ttcttttttt agttgctcaa
      601 tttgttcatt tatgcttgct gaaattgtca gtttcacttg atttttttga tcatcagaaa
      661 cattatttag ttgttcttgc caattctcat agtttgccat catttctttt gaaaaatctt
      721 ttaatgattg attattactc caagctacct gtacttgttg ccattcttgt aatttactat
      781 ctgaactctc aatttgtttt tctagttgtc tttttgtctt ttcgtatact tctaattgct
      841 tttgatcatt taattcactt tgcttcaaag cgtagcggag tgattctttt tctgacaaca
      901 tgctctttaa ttgattactg tagccaaaac tgtcatccgt tgaaaacaaa ttttttcctt
      961 tacttatact ttcaataaat agctgagcca tctttatttg ttgagtaata cgttggtttt
     1021 cttgttctat ctgagccttt tcattctcta ttaaagaaca atccaatcta accaacacat
     1081 cgtccttatg aacaaattga ttttctttta ggaagttttc cattatttta gcttctaatg
     1141 gcacttgtaa cttctctatt ttttcggggt tcaattgagc cgttgtccga ataacaacct
     1201 cttttttagc aaaggttaaa aacaatccaa taatacataa aaaaagaatt acagggtata
     1261 acacccaacg gtagaagcta tgatgttgtt tagtatagat actagaatgt tcgaaccaat
     1321 tattattctg cataaattgt ttccctctcc tagatttccc ataatttttg atagatacca
     1381 tcggcaaaac gaagttcatc gtgcgttcct ttttctacca aatgcccttc atggagaact
     1441 aaaatttgat cacatgcttt tgctattggt aaatgatgcg caataaaaat aatcgttttt
     1501 tcttttaaat tgaggagata attcataatt gcatgttcta acaaggtatc taatccacta
     1561 gttgcttcat ctaaaattaa tatgtccgga ttttttaata aagcccgtgc taaggctaat
     1621 cgttgtcgtt gctctcctga aagattattt ccaccttctt ccaacaatgt ctcgtaacgt
     1681 aatggctgtt gattaataaa ttcatttagt tggactatct cacaaacttc tattatttgt
     1741 tcgaaacttg gtgggctatc taaaccgaat ataagattct caatgattgt tccactaaag
     1801 aaaaatgact cttgaggcaa atatgtcact cgatcgcgta atatctgaaa aggaatgtct
     1861 aagtgattaa tgtttccata atggatcgat ccttctgatg cttcataaaa acgaacaaat
     1921 aactttgcaa gagttgattt tccagaacca ctaactccaa ctatagccgt tttactgtgc
     1981 gaaggaattg tacatgaaat ttcgttcaac gttggggcct tcatgctata cgagaatgaa
     2041 acgtttttta cagtgatatc ttgttgaaat aattgttcag aaagcatata ttcaaaagac
     2101 tggttcgatt tttctgaatc aatcgcgaaa atttcattta aacgattatt tgcaacttga
     2161 gccgtttgca ttttcacttg taaattaata atgttttgta aaggatctgt caaaaatact
     2221 agtaaagcat tgtatgtgat aagttgacct agactaattc tattatccat aacaaaataa
     2281 gaacctagcc ataaaattaa tgcactgcta attaattgaa tcaaatgttt aattccttgt
     2341 tgaatattat caagcgtaac cgtacgaaat gattttttca ttaatttaac aaattcatgg
     2401 tctatacggt tatatacttt ctcttcaccg ctatatgctt taattgtttc tattccttta
     2461 agactttcta tgatactaga atttacagct gcaccggctg ccatttcttc cctgttcgat
     2521 ttttcatagc ttttcacgaa agcgaatatt gcaactacat aaaaaggcaa agagcataga
     2581 gtgattaaaa acaacgtact attctgaata gctaacgtaa ccccaacaag caacaccata
     2641 ccaatatcta aaaatattga taacgtagca cttgctaaag catcaataat cttactggca
     2701 tcaagaaaac gagaaataat ttcccctgat tttctcgtag caaaaaaatt cattggtaaa
     2761 gttaacacat gtttgaaata ctgaagcata attgccatac tcattcgctg tcctaaaata
     2821 ataagaagat aactacgact atattcaaac aatacccgaa ataaatagac agcgatcaag
     2881 ccaatggaaa ttatatttaa agttgaacgt gcttgattag ggatgaaaaa atcaagaata
     2941 ccttgaaaat aatatgagct tcctatgcca aaaaacgtaa tgaaaatcga tgctaaaacg
     3001 atgtggaata ctaatccttt ttgtttccaa attataggca aaaaagaact taatccagct
     3061 actttttctt taataggaat ataagtttct ctaggcatag gtaataaaag aatacctgtc
     3121 cattcttttt gaaacgcttc aatcgttttt ctattttttc cattagcggg atctgcaatc
     3181 catagtacgt ttcccttcgt tttataaatt acaacgtagt gcatatagct gttatcaacc
     3241 aatatatgtg caattaatgg taagataaga tctctttctt tccagatctc actatctgct
     3301 tgaatagcca tacaatcaaa agacaatttt tcaaatgtct ttttcaatcc aaatgctgaa
     3361 gtaccctcta aatcagtccc agataggtcc cgtaatttat gaatcgggat atcagtttta
     3421 aaatatttta aaatcatcga aaggcaagca acaccacaat ctttttcatc ttgttgctgc
     3481 acaaaaggaa atgattttat cacttatcta acacaactcc ttctaaaaaa tttttataga
     3541 aaaattctca atatagcttt aatacagtga aaatattgtc tcataattcc acaaaaactt
     3601 tgaaaacagt tcttgaacac taactttttc attcctgaat caaatttatt ttggaaattt
     3661 tactattcaa gaataaactt gataagtttc aggaagattt ttactgttaa tcaaatacgt
     3721 atgttacagt agcaccgtaa aaaatattat ttaaagaagc gtgtttaaac atgcaaaatg
     3781 taaaggaact aagtattgtg gaaatgcaca aactgttggt ggaggcaata atgcttgggg
     3841 aaaattgggg caagttgtag gtggacttac cacaggggca gtaggtggtg caggtttagg
     3901 aacagcaatt tgcggaccag cttgcggtgt tgttggaggg ttatatggag ctgtcgctgg
     3961 aggtgctgca gctggctggg atgctcgaaa aaaataaaaa aggagaataa gtagtatgca
     4021 tattaaaaac acaaaaacaa catttatatt atcttcagaa gagttgaaaa atattcaagg
     4081 tgggagtgcc gtgggagttt taggcactac tttttctggg gcaacagcag gagtaaaact
     4141 ttgttcagcc ggaggacctt atgcaatagc tgcttgtggt gtaggtggag ctttattagg
     4201 tgctggattc agcatgtgga ccggtgcgta aacaattaaa aaatatcaaa cctcaagatt
     4261 ggtttgtgtc gattacgaac ataggtatgt tctttatctt ttcaaaagtt tggaatggac
     4321 agaacttttt a
//
LOCUS       DAHUWM010000204         4312 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHUWM010000204 DAHUWM010000000
VERSION     DAHUWM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.208"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..281)
                     /locus_tag="NK788_002434"
     CDS             complement(<1..281)
                     /locus_tag="NK788_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCH6770740.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="NK788_002435"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="NK788_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="NK788_002436"
     CDS             complement(2359..2721)
                     /locus_tag="NK788_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770741.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="NK788_002437"
     CDS             complement(2725..3063)
                     /locus_tag="NK788_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770742.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="NK788_002438"
     CDS             complement(3042..3461)
                     /locus_tag="NK788_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770743.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="NK788_002439"
     CDS             complement(3454..3687)
                     /locus_tag="NK788_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770744.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4312)
                     /locus_tag="NK788_002440"
     CDS             complement(3684..>4312)
                     /locus_tag="NK788_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCH6770745.1"
                     /translation="SQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1221 a          959 c          618 g         1514 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aa
//
LOCUS       DAHUWM010000205         4298 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHUWM010000205 DAHUWM010000000
VERSION     DAHUWM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.209"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(21..917)
                     /locus_tag="NK788_002441"
     CDS             complement(21..917)
                     /locus_tag="NK788_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6770746.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(910..1590)
                     /locus_tag="NK788_002442"
     CDS             complement(910..1590)
                     /locus_tag="NK788_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6770747.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1591..2373)
                     /locus_tag="NK788_002443"
     CDS             complement(1591..2373)
                     /locus_tag="NK788_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6770748.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2366..3283)
                     /locus_tag="NK788_002444"
     CDS             complement(2366..3283)
                     /locus_tag="NK788_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6770749.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3447..3770)
                     /locus_tag="NK788_002445"
     CDS             complement(3447..3770)
                     /locus_tag="NK788_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770750.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1258 a          943 c          753 g         1344 t
ORIGIN      
        1 ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc
       61 ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa
      121 agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg
      181 aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga
      241 atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa
      301 ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta
      361 tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa
      421 gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt
      481 tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca
      541 acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc
      601 agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg
      661 acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt
      721 tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg
      781 actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat
      841 ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc
      901 aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg
      961 tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc
     1021 aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg
     1081 atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc
     1141 attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg
     1201 cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa
     1261 aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga
     1321 aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg
     1381 tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat
     1441 caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag
     1501 aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc
     1561 gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa
     1621 cattcctaga gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc
     1681 ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca
     1741 ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc
     1801 catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa
     1861 agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag
     1921 aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta
     1981 agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac
     2041 gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat
     2101 cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag
     2161 aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc
     2221 cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata
     2281 aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac
     2341 ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga
     2401 aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta
     2461 ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc
     2521 tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg
     2581 tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata
     2641 attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt
     2701 catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga
     2761 tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa
     2821 tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta
     2881 aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt
     2941 cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg
     3001 cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg
     3061 cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca
     3121 taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca
     3181 ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg
     3241 ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt
     3301 attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa
     3361 gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt
     3421 atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc
     3481 gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat
     3541 tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa aattagactg
     3601 tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca
     3661 agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc
     3721 gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa
     3781 aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct accaaataag
     3841 aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca
     3901 actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct
     3961 tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt
     4021 ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga
     4081 atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg
     4141 ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg
     4201 taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg
     4261 gctttcttaa gacctggttt tttcttcgta tcattgaa
//
LOCUS       DAHUWM010000206         4291 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHUWM010000206 DAHUWM010000000
VERSION     DAHUWM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.210"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..734
                     /locus_tag="NK788_002446"
     CDS             <1..734
                     /locus_tag="NK788_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6770751.1"
                     /translation="YVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDYKRESKTIKIK
                     DKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVV
                     INGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTK
                     GTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENS
                     DITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(866..1213)
                     /gene="rplS"
                     /locus_tag="NK788_002447"
     CDS             complement(866..1213)
                     /gene="rplS"
                     /locus_tag="NK788_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCH6770752.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(1349..2110)
                     /gene="trmD"
                     /locus_tag="NK788_002448"
     CDS             complement(1349..2110)
                     /gene="trmD"
                     /locus_tag="NK788_002448"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCH6770753.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            complement(2100..2621)
                     /gene="rimM"
                     /locus_tag="NK788_002449"
     CDS             complement(2100..2621)
                     /gene="rimM"
                     /locus_tag="NK788_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCH6770754.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            complement(2791..3582)
                     /locus_tag="NK788_002450"
     CDS             complement(2791..3582)
                     /locus_tag="NK788_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770755.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            complement(3707..3958)
                     /locus_tag="NK788_002451"
     CDS             complement(3707..3958)
                     /locus_tag="NK788_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCH6770756.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            complement(3970..4245)
                     /gene="rpsP"
                     /locus_tag="NK788_002452"
     CDS             complement(3970..4245)
                     /gene="rpsP"
                     /locus_tag="NK788_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCH6770757.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
BASE COUNT         1259 a          851 c          764 g         1417 t
ORIGIN      
        1 agtacgtcag tgtaattaaa tccaaattga atcttctttt ttcatacgct atgaaaaaag
       61 gctatgtaga aaaaaatcct atcaacgaag tagttattga ttataaacga gagtcaaaaa
      121 cgataaaaat taaagataag tttttagaag atgatgaata taaaagacta gtaaatttca
      181 caacttcaca caataaaaga tattctctcc ttttccagtg gctatatttg actggaatga
      241 ggcctggaga agcaatcgca ttaagtaaag acgatgtaca cattaccaat aataatgcat
      301 cagtagttat aaatgggacg atgatgtata gagaacgttc aatagctgat atgaaaaaat
      361 ctgattctac aaaaactgct gctggaatgc gagaaattga tttaccaaaa aaagcgatag
      421 ctatttacaa tgagcttcta gaattaaatc cgaatggtca atttctattt caaacaacga
      481 aaggaactcc tttccaacta acagcaatta acacctattt gagaaatcat aaagctgaca
      541 tgaagattga taaaaaactt agttcacata tttttagaca tacccatatt tcaaaattag
      601 cggaactagg aactcctctg tatgctattc aggatcgcgt tgggcacgaa aatagtgata
      661 tcactgaaaa gatttatttg catgtgacaa agggagtaaa agaaaaattg aaagaagata
      721 tagaaaaact gtaacttgtt gccccttttt tgcccctttt tattaatcaa aaatgtccca
      781 aatagaaatc gttaatataa aaaaacagag aacaaaaaaa gaacccttga ataccaaggg
      841 ttctttctca gtctgataac gatgattaac gacggatttc tttgatacga gcagcttttc
      901 cgtgtaatgc acgtaggtag tacaattttg cacgacgtac tttaccgtag cgaactactt
      961 cgatttttgc aacacgtggt gtatgcaatg ggaatgtacg ttcaacgccc actccgttag
     1021 aaactttacg tactgtataa gtttcgctga ttccagcacc gcggcgtttg ataacaacac
     1081 cttcaaataa ctggatacgt tcacgagtac cttcaacaac ttttgcgtga acacgcacag
     1141 tgtcaccagg gcggaacgct gggatgtcag aacgtaattg ttcttttgtt aattcttcga
     1201 ttaatggatt catggttttc ttctccttat tccaaatatt cttatgagct tttctcacag
     1261 cggaatatcg taatagttaa gtgcttttgc actcaccata aaatactaac atattatcaa
     1321 cctactgtaa agaagatttt agaaaaagtc acttggcttc atctgctgtt ttttcttgtt
     1381 cttctttctt gatttcttct aaaaatttcg tcatttgagg tgtccattca agtgtttcca
     1441 aaagatcagg ccgacgttga taggtccttc ttagtgattc ttttaattgc cattcttcaa
     1501 tcaatttatg atttccattt gtcaatactt ctggtacttt catcccttta tattctgccg
     1561 ggcgagtata ttggggatgt tccaataaac cggttgaata agaatccgtt tgagctgaag
     1621 tctgattccc taacacatct ggcaaaagcc gaactgttgc atcgatcatg accatcgcac
     1681 ctaattctcc cccagtaagg acataatctc ctaaggaaac ttcatctgtc actaatgtgc
     1741 ggatacgttc atcatatccc tcataatgac cacaaatgaa tactaagtgt tcttcttgtg
     1801 aaaattcttc tgccattttc tgatcgaatc gtttaccagc tgggtcaaga agaattactc
     1861 tttttttgac atccggtgca gctttttcaa tggcttggat gttatcatag atgggctgga
     1921 ctttgagcag catacctgct ccaccgccgt atggataatc atctactgtc tgatgtttgt
     1981 tatctgaata atcgcggaag ttagagatat tgatatctaa taatccttta tctacagcct
     2041 taccgatgat ggattccccc ataggacctt caaacattct tggaaataaa gtcaatacat
     2101 caatcttcat cgtcaatcag tccttctgga atttctacac ggaccactcc agcttctaaa
     2161 tcgacttctt tgacgacaga agcgatatat ggaagcaata tatcagcttt tttcggacgt
     2221 tgaacgaccc aaacatcatt ggcacctggt gacaagattt cttttacttt acctaactca
     2281 gttccatctt ctaatacatg cagaccgatg atctcatggt aatagaattc attttcagct
     2341 agatctacag agttgtcttt tgacactttt aatatgccat cccgatattt ttccacatca
     2401 ttgatagaag gatgtccttc aaaactcaat aagtcaaaat ttttatgttt gcgatgactt
     2461 ttgactgtta attccacagg tgcttttccg tcttggaata gtgttagcac cgttcctttt
     2521 ttgtaacgtt cttctgggaa atccgttgtt gagataacac ggacttcgcc tttgatacct
     2581 tgtgtgttta cgatttttcc tacattcaag tattcagtca acttatatcc ccatttcttt
     2641 tttgtctgtt ttattttagc atgtgtgaag aggcaattct agttttcttt taaggtatta
     2701 agccaaaatt atgcaaaaat gcggcgtgct atttttgatt acttcctgtg acttggagtt
     2761 aaatgatggg ctcagatcag cgcgctgatt tcacaacctc ttcatagaaa ctgccatctt
     2821 caacgcagac ataggatctt gatgagtgat cgtataggca tttttcggtt cttgccagct
     2881 tacttctacg gctcgattcg tcgtatcacc catatcaaca gtaattttac caaattgttc
     2941 aggtgctggt agactgtttt cttccaaata agctacgatt tctttagcta atagtaaggg
     3001 atcttggcct tctatattat tcccacggat tcgaatacgc cagtttcctt cttgccattc
     3061 aagaaaactt gaacctgctg caccttgctg ataccctgtg attcgagaac ctaagtccac
     3121 ttgctgacca tttgtgtcga tctcaaatgg ctgcaaaact tggattgttt cttgactaga
     3181 agcaaaccca tattgatata gataggttgc aataggcgtt tcttgattca attcgtcagc
     3241 attcaacccg aggggacgat ctgttctgta atataaaata gaataacctt gctttgtatg
     3301 tgtcgctgcg atattgagat agcctgaaga gactggtaca tctagaggca tgggaacatt
     3361 tggctgttgt tctttcaact ggtctaaagc agtctttttc tcattatttg aggtagccat
     3421 tgtttgttca gtggtggaag ccgttttggt actacttttt tctggactgg taacagtttc
     3481 agatatactg cttgatagat gtgtattgtc tgattcttga gtaaccgaac tacaagcgct
     3541 taacaataga gcaaatggaa taaataacat tttctttctc attggctgtt cactccctcc
     3601 tcctataagg atacgctttc ttgcttgttt tgccaaataa tcatttcttc aattaataaa
     3661 aaaaacaaaa aagctgcact cgaaaattcg agtacagcac gtttatttac ttttccttat
     3721 catcgatgat attcaaacgc acttttttcg gtgcattgat ccgaacaccg taaacgattg
     3781 tgcggattgc tttagcaaca cgcccttgtt ttccaatgat acggccaata tcttcaggag
     3841 ccactgttag attgtactcg taaaagtctt tcgactctac gacgtctaac ttgacttgat
     3901 caggataagt gactagcgga cgaacgattg ttaagactaa gtcgcttaaa tctttcatat
     3961 ccggtcacct tatttctttg agaatttagc ttcgtggtgt ttttgcataa cgccttcttt
     4021 tgaaaggatg ttacgtacag tgtcagaagg ttgtgcacct tttgataacc agtcaagaac
     4081 taagtcttct tttaaaacta cttctgcagg gtctttcaaa ggattgtaag tacctacagt
     4141 ttcgatgaaa cgtccatcac gaggagaacg tgaatcagct acgacgatac ggtaaaacgg
     4201 actcttttta gaacccatac gttttaaacg gattttaact gccattattt atttccacct
     4261 ccattgtttt attcacaaga tgtagtgtaa c
//
LOCUS       DAHUWM010000207         4278 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHUWM010000207 DAHUWM010000000
VERSION     DAHUWM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.211"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(9..530)
                     /locus_tag="NK788_002453"
     CDS             complement(9..530)
                     /locus_tag="NK788_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCH6770758.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..>4278
                     /gene="nifJ"
                     /locus_tag="NK788_002454"
     CDS             729..>4278
                     /gene="nifJ"
                     /locus_tag="NK788_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCH6770759.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPE"
BASE COUNT         1332 a          803 c          945 g         1198 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagagg
//
LOCUS       DAHUWM010000208         4238 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHUWM010000208 DAHUWM010000000
VERSION     DAHUWM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.212"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..319)
                     /locus_tag="NK788_002455"
     CDS             complement(<1..319)
                     /locus_tag="NK788_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770760.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTE"
     gene            complement(527..1453)
                     /gene="glsA"
                     /locus_tag="NK788_002456"
     CDS             complement(527..1453)
                     /gene="glsA"
                     /locus_tag="NK788_002456"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCH6770761.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1476..2903)
                     /locus_tag="NK788_002457"
     CDS             complement(1476..2903)
                     /locus_tag="NK788_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCH6770762.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2934..4148)
                     /locus_tag="NK788_002458"
     CDS             complement(2934..4148)
                     /locus_tag="NK788_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCH6770763.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1325 a          907 c          758 g         1248 t
ORIGIN      
        1 gttctgtact cgtgaatata ccattaaaat aataacttaa aaactttaaa aaataagatt
       61 gaccgtacag aaactgtgtg agctcagaaa gagcaatacc atgtttgaat acgagtttat
      121 acttatgctt ctctcttact tgttctttta tacaatattc tagacactcg gctagacttt
      181 ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga aatagctgtt tgttcgatgt
      241 ctatttcttg gaattcaaat aattgttcac acagatacac ttttctaaat atatttcgct
      301 cgatgtaatt ttccagcatt cctgatacac gcctccagtt tttcttatga ttttatttaa
      361 tcatatctgt aaatcgtatt tttgtttcct ttttatttag aaaagccctt tcaaaaacca
      421 ataaaaaaag aaactgggac aaaagtcaaa caagctcaca atttcagcct cattgtaggt
      481 agctttgagc cgtttggact tttaccccag cctctttcaa gaatggttat tcaaagatgt
      541 cagcgtctag ttctttgact acatctttca acaattggat accagctgcg ctgttaccaa
      601 atggatctag tgccgggctg aagacaccta tgccgtaacg atttggcaca gctgccatca
      661 acccgccgcc tacaccgctt tttgcaggta caccaacatg gacggaaaac tctcctgatt
      721 catcatacaa accagcagtt gtcataattg atttgacgat tgtagcactt tcttcagtaa
      781 tgatttgttt gccattccaa ggagcaattc ctttatttgc cagcacagca gctaattttg
      841 ctatgcctgt agcggtgaca ttgagggagc attgtctgaa ataagcatcg agtatctccg
      901 gtacgtctcc ttcgatcatt tgattacctt tcatatagta agcaagagag cgattgatat
      961 cacctgttct tgattcagaa tgatagattt cttcattcaa tgtgatatta ggatcatcac
     1021 agatctcttt cataaatgac aagattcgct caaatttttc atctgcatct ttaccggcaa
     1081 tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg attcattgga tgtttgcgat
     1141 gattgatttc catattcatg atagaattga aggcaaaacc ggttggttcc gtgttgattg
     1201 tttgaaagac tttttctatt ccgttgtctt tgattgctaa taacaatacg ggaacttttg
     1261 acatgctttc gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat
     1321 ccaaatcata aatcgccata cctaattgtt tgggatccac ttcagctaat gctggaatgt
     1381 aatccgccac ttgtcctgtt tgataatagt ggaaattttt tccaatcaat tgttctaatt
     1441 cttttacttc catttgaata accagctttc attttttatt tttctgcatg ctggaattta
     1501 tgacggcatg cgtagatcgt aaatggaata actaagacga taagataaga aacaatcaga
     1561 atcgtttcat aaatcccatg ctgtccagct gcgatactag caggtggaac aaatgagata
     1621 caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa cgattacttt tacaactttt
     1681 ccgcctggaa cctgataggc tctttcaaga ctgtcatctt taaaaaccag cttcaaatag
     1741 cccgcgaaaa acataaagta caacatcagg tagattacaa cagtcaatga catcgctgca
     1801 agaaatgaaa gattattccc cccgccgctt aatgtaaaga cggctgccca gattgttgca
     1861 acgatcccat ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa
     1921 tattcaggaa gtaatccttc ttctgctgct acgtgaagtg cttttgaagg agaggttacc
     1981 caagaagcaa tttctccgat gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc
     2041 catgcaagat ttggatcaat gtgtcttaat aggaattcta acgattgaat cacccctgta
     2101 ttaagagaca aaccatgttg aggtactgta gtagcgacgg tcaatccgcc aaaagtatct
     2161 aaaatgattg ccagaataac caatacaaac attgctaatg gataatcctt tttagggttt
     2221 tccatttcat ttgcatgact ggcagatgtt tctacgccca tatatgctaa aataaaggaa
     2281 acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt catagacagt
     2341 atcgtttgta gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa caagactaat
     2401 gctggcaaca agataccagc tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt
     2461 ccttttaact gtgagagtaa aatagcccag aaaataatca ataggattgc caatttcaga
     2521 tatttattgt tttgtatagc accaataccc gttgcatagg ataatgcacc agttaagaaa
     2581 tacagcatgg tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa
     2641 ccgaatcgtt ttcccaatgt gcgactgacc caagtataga caccgccttt ttcccatcct
     2701 ttcaccgttg ccatctctgc tgcgcacaat gccacaggga taaaccatag caaaccgcct
     2761 aataacaaga aaaatactaa gctgaagcct gaggtcgcga atgtgggata ttcatataat
     2821 gaaacgacca ttgagatggt catagccaaa aagccaaaga acgttaattt ttttgcagga
     2881 attttcgttt tgctttgttg catgaatctt cctcttttct atttgaattt gtttcaattg
     2941 agtttgttgc caaagaagaa aaagtccaat ccttcttctt gttcttcttt tgtagtagtg
     3001 atcgttgtaa agtgcatcgc cgattttaaa atcactgttg tcagcacata ggcatacacg
     3061 gcagtaatga tgacaccaat cacttgaatc cctaagaagt accagctgct tggattcgtt
     3121 agtaaatggc tgttagatgc aaagatccct atcagaatcg tacctgtgaa tccgcccatg
     3181 ccatgaacac cccacacatc aagtgcatca tcccattgtt tctttttacg aaaatcaaca
     3241 gcagcatgac agacgatacc cgcaatgatc ccaatcagca tggcactttt tgcttctaca
     3301 taaccggcac aaggcgtaat ggctgccaat ccagcaacgg atcctgttag tacgtcgaca
     3361 aaatcaaagc catttccttt tacttttgca atgatcagcc aagtaatcat cgcaaatgca
     3421 agagcgatga atgttgaacc aaatgcattg actgcttgtt catctgctcg aagtgcacca
     3481 ccggaattaa aaccgaacca gccaaaccaa agcagtcctg taccgatagc agctgccata
     3541 agactgctat gctgacttct ttctcctaaa ggaatctttc tttgaccaag tgtgaggata
     3601 catgctaaag agccgaatcc agctgtcgtg tggatgactg ctccaccagc gaaatctcta
     3661 aatcccatct gatccaaaaa tccgccaccc catatccagt ggcaaacagg gaaataaatc
     3721 agcagattcc aaaaaataac aagtataata tagcctttta aattcaatct ccctgcaaaa
     3781 gctcctgtca tcaaaggaac ggtaatcacg ataaacatca gttggtaaag aaagaacatt
     3841 aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa
     3901 aagaaatctg ccgggtttcc aataacaccg ccgatatccc gtccaaaggc caatccgaat
     3961 ccaccaaata cccacataat cgttgttatc ccaacggaga gaaaagattg agacatgatc
     4021 gttagtatat tattcttccg tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc
     4081 aggcaaacca ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt gataggtaat
     4141 gtattcattt ttttcgcctc ctttctacga ttacgagtat aaaagaaggt gtagttgttt
     4201 ttttcttctg tttttttgaa ttaatacttg catttata
//
LOCUS       DAHUWM010000209         4234 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHUWM010000209 DAHUWM010000000
VERSION     DAHUWM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.213"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(141..1088)
                     /locus_tag="NK788_002459"
     CDS             complement(141..1088)
                     /locus_tag="NK788_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6770764.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1334..1798
                     /locus_tag="NK788_002460"
     CDS             1334..1798
                     /locus_tag="NK788_002460"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCH6770765.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1860..2903)
                     /locus_tag="NK788_002461"
     CDS             complement(1860..2903)
                     /locus_tag="NK788_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCH6770766.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(3280..4233)
                     /locus_tag="NK788_002462"
     CDS             complement(3280..4233)
                     /locus_tag="NK788_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCH6770767.1"
                     /translation="MKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIF
                     IFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVI
                     FQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISL
                     ICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALF
                     ALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLD
                     FLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         1447 a          809 c          811 g         1167 t
ORIGIN      
        1 tctcaaattt ttgtgaaatt tcggcttatt tccgaagaag ctacttctgg aacaccattt
       61 attcggaaaa aaggactgtg acaagacttt tgtcacagtc ctttttcatt taaaaaaatg
      121 tttacttcat ttgacgtata ttattggtga ggcttcaatt catttggctt cactttttct
      181 gtttctctta tgatataaac aggacgctgt ttcgtttcta agaagatttt cccaatatat
      241 ttcccgatga ttccaagaca taacaattgg attccaccaa tgaacaggaa gatcgagacc
      301 atggaaggcc atcctgatgt aggatcacca aacaacaatg cccgcaaaat aatgaacaaa
      361 attgcaatac cagaaccgac acaagacaaa gcgccgataa atgaagcaat cgttaacggt
      421 gcatccgaga agttcacgat accatcaatc gagtagttga ataatttcca gaaggaccaa
      481 gatgtttcgc cagccacacg ttcgcggttt tcataagaaa tatattctgt tttaaatccg
      541 acccagctaa ataatccttt tgaaaatcgg ttgtattctg ttaattctag gacggcatcc
      601 accatttgac gagtcatcaa gcggaaatca cgagcgccat cgaccatttc tgtatcgctg
      661 attttattga tcaactggta gaattttttc gcaaagaaac tgcgtatcgc aggctcgcct
      721 tcacgagtcg aacgccgggt gccgacacaa tcgatatcag aagtagtgat ccggttgatc
      781 atctcaggaa gcagttcggg cggatcttgc agatcaacat ccatcacggt cactaaatct
      841 cctgtagaag cttgcaatcc agcgtacaga gccgcttctt tcccaaaatt tctggaaaat
      901 gagacaaagc ggacataagg ataagctgag gctagcttct tgattgctgc taacgtaccg
      961 tcttttgatc cgtcgtcaat aaacaaatat tcgaactcat gggcaccttt gaatttttca
     1021 acctcatcaa aaaaaagagg aacagttttt tcttcattaa aacatgggac aacaattgat
     1081 actttcatcg tttctatcct ttcaatcgta tgtttatata tggtttacat tcaaaattta
     1141 ataaacctta aataacacct ttaaaatttg attttacgta aaagaacagg tattcttttt
     1201 gtaaactaac gtatttagtt taccatgttt cctcggttta tcaaactttt aagaacattt
     1261 tttattagtt ggcaagtagt tttggataga gaaaaaggag gaagttccag tctattgact
     1321 aggagcaaac aaaatgaaaa tacacacacc tatcggcttt ttatggttag atgcagatga
     1381 atccggattg actgctgtat cttttcagcc aatttcagaa agcgaatcag cgaatcagcg
     1441 aattttaacg cgcacacaag aacagtttga agaatatttt gcgggaaaaa gaaaagaatt
     1501 tgatgttcct ttatctataa agagaggtac gccttttcag caaaatgtct ggaaagcttt
     1561 gtcgaccatt ccttatggag aaatccgaac gtataaagaa gtagccgttg ctgttgatag
     1621 tccgaaagct gtgcgagcga ttgggcaggc aaatcgaacc aatccattgc caatcgttat
     1681 tccttgtcat cgagtgatcg ggcaaaatgg aaaactgaca ggatatatgg gaaacgcaat
     1741 agatggggtt gaaatcaaac gccaattgtt gttgttagaa ggatatctgc aaaattaaaa
     1801 acagttgtga ttcggacatt gttccatgcg agaatcacaa ctgttttttc atgttcattc
     1861 taaactttga tcagccaaat aaatagtaaa gccatcactg tttggagaag cccgacagct
     1921 aatccataag ataagaagcg tgtaccttct ttcatgaatt ttgcaaagtc taaacgcaga
     1981 ccaattgccg ctaaagcagt gatctcaaac cagctgctga taaaatgtgc cgattggtca
     2041 ataactacag gtaaagaaaa caagctgttg accaaacaaa agatcacaaa ccctaataca
     2101 taccaaggaa gcggtaattt tcgttttttc tgcgtatcag ttggcgtttc aaacagagct
     2161 ggttgtttct tgtgcatgaa tttttcaaac agatagacaa caacaacgag catcataata
     2221 cgtagaatct taaatagcat ggcgaattcg acaacagaat tgtcaagtaa gcttgcccca
     2281 gcgactactt gaccaacaga ttggagtgta ccacccaaaa gtgcactttt ttcaatgaga
     2341 tttccaccaa acaactgtaa tccaagaaaa gggagagtca acatcatcac agtacccagt
     2401 agattgacta gtgtgatgat ctgtcctttt tcttcctctt tcgcatggat ggaaggggcg
     2461 attgaagcaa tagcagaaga accacaaaca gcgtttcctc cagccatcat caaagaagaa
     2521 gtatcgctga attgcagttt tttaccaatc aagtaagcac cgatgatagt gactgacatt
     2581 tgcagtaaca caaaaacaat tccttttaca ccaatttgag caatcgtttg agctgtgaca
     2641 gttgcaccta gtaaaaaaac agagaattct aatagtttgc tttcagcaaa ttttgtgccg
     2701 attgctagaa caggctgttt aaaaaaagta tttcctaata aaatacccaa taaaatagca
     2761 atagtcgctc cacctaactg tggcagataa atagcaagaa gcctgctgaa ggcagcgatg
     2821 gcaaaagaaa gcagtaaacc gggtaagatt tcgcggaccg ctgaatcctt tacagatttt
     2881 attggatgtg acaatgattt catttgatcg gttcctttcg tcaataaata ctaaaagcat
     2941 actcgaacat agtagcacta aaccagtatt ttgacaaaag aacaagaggt cgtgacaaaa
     3001 gtcttgtacg acctcttgtt tcgaataaac ggtgttccag aagtagcttc tacggaaata
     3061 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     3121 gctgaccact tcttttacga cctcttggta aacggtgttc aaaagcttgc atctgcggta
     3181 ataagcccaa ccagtacaaa aacatgaaca gctgtttttg tttggccgtt cttattactt
     3241 gatgcagaac agctttttca caacctattg aatcgattat taagcgattt ttttagaaac
     3301 aaattttcca tgtttgctat gttctttaat taagaaacgt acgtaatgag caagaacgta
     3361 gaagacaact acacttgcaa acagacattc cgcaactttt aagaaatcca atacgctact
     3421 atagatacct aaactgttga agtaatgaac agtagtaaat atagctgttg cagagataac
     3481 aagcgggaat gtaaaggctg catagcttgg ataaaaacga agcttgaaca aacgtggaag
     3541 catagacagc acaacgaaat acaatacttg agataaagcg aacaatgcta cgacaagata
     3601 aaaattaggt tcagcaaagt ttttaagata acctgttagg caaagagaac caggtgctgc
     3661 aacgatcgta atcagcggac gtgtcggatg agccatattt cttacgaaaa agacacgttt
     3721 gatgatgatc ggcaataaga acgcatagca gatcaatgaa atccaaaaga aaatttttcc
     3781 aataagtggg aagaagttcc cggcagttac agtgatgacc ccaaatccaa cgtaaatgat
     3841 aaaccagctt ggataaattg cttcaattga aagatcagct ttgataacat ggtgataatt
     3901 gaaataaaag acaaggatca cttggaagat aacagcgcac agccaaatga atttgacaat
     3961 tggtgcaata ctttcatatg aaacaaaata agtacaaata accatgagtg acatcgtaaa
     4021 agttggtgaa acggaggcga tgattggatc atgcaacgtt tgtttcgtgt gttcgaataa
     4081 gataaacaat ttaacaaaaa tgaatatcat aagaatcata gagataccgc cagttagatt
     4141 gcctaaagca acatggtgat agtctttaaa cagatttcca agggaagcta acccaaggat
     4201 caacccgcag atcgggatcg gaataatttt caaa
//
LOCUS       DAHUWM010000210         4218 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHUWM010000210 DAHUWM010000000
VERSION     DAHUWM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.214"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(82..1848)
                     /locus_tag="NK788_002463"
     CDS             complement(82..1848)
                     /locus_tag="NK788_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770768.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1808..3562)
                     /locus_tag="NK788_002464"
     CDS             complement(1808..3562)
                     /locus_tag="NK788_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6770769.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3672..>4218)
                     /locus_tag="NK788_002465"
     CDS             complement(3672..>4218)
                     /locus_tag="NK788_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCH6770770.1"
                     /translation="IGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVY
                     ISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQ
                     VASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLV
                     VASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1427 a          800 c          594 g         1397 t
ORIGIN      
        1 gctgtttaat aaaattattc tttttgaaaa agaattataa ctatatccca aaaattgtag
       61 gttaatttaa agcctatttc atcatcgtga ttgagcttct ttttcataga cgaatttctc
      121 agatagttta gttttcttta tgagattttt gtatatttct gaccttcgat acaactcatt
      181 atgagtacct tgatcaacta gttttccttg atccatcact acaattttat ttaccttttc
      241 aatagacttc aagcgatgcg atataattag tactgtctta tcttgtatta aacgatttaa
      301 actttcctga atacgttttt cattttccgc atctaaactg gcagtaattt catctaaaat
      361 taaaattggc gcatttttca aaaaagcccg tgctatggat aggcgttgtc gctctcctcc
      421 agaaagtgaa cttccgtttt ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat
      481 aaagtcttcg cagttagcta aacgagctgc ttttttcact tcctcatctg acgcttgact
      541 atttccaata cgtatatttt ccaaaatact tgtattaaac aaagttacct cttgaaaaac
      601 aatagcgatt ttgctaaaca aagaagctgg agatacacgt ttaatatcat acccatcaat
      661 taaaatacag ccttcatcat aatcatacag tctagacact aatttcaaga tactagtttt
      721 cccggaacca ctagcaccta ctaacgcagt cacttctcct tgtttagccg taaaagagat
      781 atgatctaaa attggggtat tgttatcata tgagaaagag acatctcgca actcaatgtc
      841 aaaagatttc agaggactat ctgatccctc ttggatactg gtttccttca tagctctaat
      901 tcgctgtatt ttaggagcta aataaaatat ttcaagcact gcttctttca tagaatcgaa
      961 ggaatccttt atttttattg cggctagtaa gtaaccaacg acgtagagta tagtcacttc
     1021 acccgttaac agtaagtgaa cacctactag aatgactact gctaaactaa catatgagaa
     1081 aatagaggat aaggccatga ctgaaaaagt acttaattct acctttaaat gaatcctttc
     1141 actctcttcc atttttttga aaagtctgtc ttgaactttt ttagatagat taaagctatt
     1201 aatctcttgc tgcaattcta tagtttcttg aaattcttcc gagttttctc tcaaagtatc
     1261 gtaatattta gtattggctt tcgtttggga tttctttgat aacaagatta agacaaaact
     1321 aaataacgtt ggcaaaataa cagctaatcc cattttgaca ttaccaacca aaagcatcac
     1381 tgaaataaaa gggaaaaaca gagccatacc gccggcttta ggtattgcat gactcatcgc
     1441 atgctcaata ccttcaacgt cagacatgat agtttgagat aaatctgata aattatgttt
     1501 tgaaaaatag gataatggta aatttgacaa atcgtctgca atttgcactc ttaaatgcgc
     1561 actttcttta taagtactat tatataagtt ttcatactcc ctatctaaca accaatacat
     1621 gaaaagcaaa gtaacaaacg atacggcaaa ataaagccaa cggggctttg tattttctaa
     1681 aactaattcg tctatcagca tcattataat gaatgctggt accatattaa tgcagtatac
     1741 aaaaaaacta gaaatagaag ctttagttaa agcttctgcg ccctgatcac ttaaagcaaa
     1801 tttgttttta taaaaacttt tcattgtcaa gcctccaatc atttgcattc tcataattag
     1861 tgaccaattg tttatacact tgatgtttag ccattaattc attatgattt ccacgttcta
     1921 ctattttccc ttctgacaag acaagaattt catctacaga tttaatgctg gataatcgat
     1981 gagctatcat aataactgtt ttttctttca tcagatgttg aaaagccttt tgtagttcat
     2041 actcattatc tggatcaata gatgcactgg cttcatccat aataacaatc tttgcatctt
     2101 ttaaaattgc tctggcaata gctatacgtt gtttttctcc tcctgacaga taaactcctt
     2161 tactaccaat taaggtatgc tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg
     2221 ctaatcttag cgcgtcaaat actttacttg ctggcgcttg aggatttgct atagaaacgt
     2281 tatcgtaaat actctgattg aatagtttcg tatcttgaaa gacaaaagca attgtgttaa
     2341 taattgtgtc ttttgaatag tgatttaaag gcttaccacc aattttaatc tgtcccttat
     2401 ccacattgta gaaacctgaa attagtttag ctatcgtact ctttccgctt cctgattttc
     2461 ctactaatgc ataaattctt ttttgttcta aaactaagtt gaaattttct aaaacacact
     2521 cttctttgta tgaaaagctg acattttcaa atgcaatgtc acaattttta atctgtgtct
     2581 cgttcccata ttttagttgg tcttgcttca tttctaaata aagggagtct agtgtttcga
     2641 ctgcatatct tgcttcaaac aagtgcatac ttacatacat tattctcatg aaagaaacaa
     2701 aaataattcc acttaaaaaa aacaacatca acaagtcaac agctaatatc ttgggagtgg
     2761 taagagaagt tataaaaaag gttataggaa ctatcaaaat gcaaactatg ccaaaaaata
     2821 gccattgaaa aagaacaaaa ggtgttttcc cactcaaaga atacttatat gcaaacttag
     2881 cataatcgtt aattgcttta ttcaaggctt taaatgatgt tactttagca ccaaatatct
     2941 ttataacttg aattccgcgg atatattcta ccgtttcaga gcttaacaca tctaacgatt
     3001 cttgatattt ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa
     3061 ttactaacat ggcgattaga accaatccaa ttttaaagtt aatgaaaaag cctaaggcaa
     3121 ctgataaaac aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca
     3181 tatgtgtttt agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat
     3241 caaaaaaacg aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct
     3301 ctaagcgaaa cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctccta
     3361 aagttaacca agcaacagtt tgaaccgcat aactcttagc taaatattca tctcctacaa
     3421 taattagaga ctgaagaaat ttgtaaatac tgtaatatcc atagactgtt aatacgacag
     3481 aaaaatcaga aaataaagtg gctaaccatc cgtagtattt cattttaggg acataactaa
     3541 ataatttttt gtatactttc attttttcct cccccgcatt caatttcttc tcttattcgt
     3601 ttacaactgt taatgctttc cgtaatagcg taaattagag atgtacaaac agataaaagg
     3661 taaactacgt tctatttttt gtattttttt aagacttttc ttccaatcaa tacaccaatc
     3721 atagataaac ttgctacaac taacactgaa atgattcccc atatacctac cgcatagtgg
     3781 atcattgcat ccattgttgc agcagattgt cctttatcta tccaatcttg acgaaaacga
     3841 tctgtaaaga accatatagg aatccactgt ccggcagcac tgcctaattg cattagagcc
     3901 caagacgcaa cttgaggaat tgctcgatca tatccaaata cagatagaat aatatcagct
     3961 aagatcgcca tactaatcga actgataaac caaggtaagt acgccccgcc cattaacgta
     4021 aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc cttttctagg gtagttatag
     4081 ctaatataaa caaatatgat tccagataat aatccaaaaa tcccggggga tatgctgtga
     4141 ccaaacgccc caccaatact actaaccatt attgaaaaaa agtaaattac tagggaaata
     4201 actgtagcta aaccaata
//
LOCUS       DAHUWM010000211         4202 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHUWM010000211 DAHUWM010000000
VERSION     DAHUWM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.215"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(1..353)
                     /locus_tag="NK788_002466"
                     /pseudo
     CDS             complement(1..353)
                     /locus_tag="NK788_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(613..1161)
                     /locus_tag="NK788_002467"
     CDS             complement(613..1161)
                     /locus_tag="NK788_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770771.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="NK788_002468"
     CDS             complement(1162..2019)
                     /locus_tag="NK788_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCH6770772.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="NK788_002469"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="NK788_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="NK788_002470"
     CDS             complement(2581..2910)
                     /locus_tag="NK788_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCH6770773.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="NK788_002471"
     CDS             complement(2987..3259)
                     /locus_tag="NK788_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCH6770774.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="NK788_002472"
     CDS             complement(3259..3522)
                     /locus_tag="NK788_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCH6770775.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3628..4044)
                     /locus_tag="NK788_002473"
     CDS             complement(3628..4044)
                     /locus_tag="NK788_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770776.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4050..>4202)
                     /locus_tag="NK788_002474"
     CDS             complement(4050..>4202)
                     /locus_tag="NK788_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6770777.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1593 t
ORIGIN      
        1 aaatgttctt actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg
       61 atctcgctta ttataatcgc tttcatttat cctaagagtc aaatcttttt ccaccataac
      121 ttttacaccc aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt
      181 taaactgaca attagctcat tgggtacata cttatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatttttat taacaagttt
     3661 tatcacaggc ttcatatatt tgattataac ttcatcttct acgtagatat ctgttatcat
     3721 tacgacatct gtactatttc gattgagtac tttcacttga tctccagcat tgattttccc
     3781 tctaaatctt ttaggaatga accaactata catatgatta gcatcaccaa aatgtgcttt
     3841 tatcgcattc tcaggatata acttattcaa tagttcattc tttttaattt tcaaaacttc
     3901 aaatctatac ttatcatcat ctgctaatct aaataaatct tctaaacgca tgttttctaa
     3961 ctgtaagttt tgaaaatctt ccggtataaa ttttcggttc tgttgcgaag tttgaaaatc
     4021 aaacgcgccc tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt
     4081 tcagtagaca ccgaaaagct gaagagcgtt ccatttcttc ggttcttttt atatattcct
     4141 cgaatggtct ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta
     4201 tt
//
LOCUS       DAHUWM010000212         4158 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHUWM010000212 DAHUWM010000000
VERSION     DAHUWM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.217"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            74..1375
                     /locus_tag="NK788_002475"
     CDS             74..1375
                     /locus_tag="NK788_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770778.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1402..1662
                     /locus_tag="NK788_002476"
     CDS             1402..1662
                     /locus_tag="NK788_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCH6770779.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2661..3896)
                     /locus_tag="NK788_002477"
     CDS             complement(2661..3896)
                     /locus_tag="NK788_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCH6770780.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1195 a          669 c          866 g         1428 t
ORIGIN      
        1 taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa
       61 aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg tcccaccaat
      121 ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg tttcaacatt
      181 gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt ttcctggatg
      241 gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga tgacgatggg
      301 attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat catatggatt
      361 agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac aagttcctgt
      421 aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga gttctttggg
      481 agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag tatatcgttt
      541 ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg
      601 tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc tgattcgaag
      661 cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt taaccaatat
      721 tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt tgttgtggtc
      781 atttggtatc catggcatga gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat
      841 gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat taccctatat
      901 cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag caacgatcgt
      961 ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt
     1021 ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag ctcctatgat
     1081 gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga ctattatcag
     1141 ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa catggacatt
     1201 accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg ttatcttgat
     1261 catgatcaac acattgatag catttattat ctattatcca tttgtaaaag cttatgataa
     1321 aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg
     1381 gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt tatcttttta
     1441 ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca aatcaatcga
     1501 tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat tgtaccgcat
     1561 atgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc tatcaatttt
     1621 atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg
     1681 taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc ttcttaaatt
     1741 tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt
     1801 tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag ccctttttta
     1861 cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa aatggaaatt
     1921 ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag tcaaatatag
     1981 atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt
     2041 atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg ctgggaatag
     2101 caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata gaacattctg
     2161 acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat gcatcggatt
     2221 gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag tggtaaatcc
     2281 tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca aagcgatgct
     2341 tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat ggtctttctt
     2401 ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg actaagaata
     2461 aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt taaggatgat
     2521 tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca ctatttactt
     2581 tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt ctctattaaa
     2641 aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc gatttcttga
     2701 attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga
     2761 atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt cactggtttt
     2821 gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct ttgctcaaat
     2881 tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac ggtcccttcc
     2941 ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga cagatattct
     3001 gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc aataggtgca
     3061 ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga
     3121 ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg ctttcgctgt
     3181 ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat tggaccaacg
     3241 attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga
     3301 tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat ttctttttgt
     3361 gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt ttttgtccag
     3421 tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat cagcgcatgc
     3481 tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa aggaatcttt
     3541 ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag tcgaggctca
     3601 aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc tttggtaaaa
     3661 gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg ttttttcgta
     3721 aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg agcaaaagtt
     3781 ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc aacctcaatc
     3841 attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt cttcatgaaa
     3901 tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa aaaacacagc
     3961 gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt tcacaacttc
     4021 taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac gcaacaattt
     4081 tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt aaaaataaac
     4141 atgagaacat aaaaaaat
//
LOCUS       DAHUWM010000213         3987 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHUWM010000213 DAHUWM010000000
VERSION     DAHUWM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.218"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(7..549)
                     /locus_tag="NK788_002478"
     CDS             complement(7..549)
                     /locus_tag="NK788_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCH6770781.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            684..1073
                     /locus_tag="NK788_002479"
     CDS             684..1073
                     /locus_tag="NK788_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770782.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1077..1841
                     /locus_tag="NK788_002480"
     CDS             1077..1841
                     /locus_tag="NK788_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6770783.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1970..2917
                     /locus_tag="NK788_002481"
     CDS             1970..2917
                     /locus_tag="NK788_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6770784.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3047..3787
                     /locus_tag="NK788_002482"
     CDS             3047..3787
                     /locus_tag="NK788_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770785.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1322 a          683 c          810 g         1172 t
ORIGIN      
        1 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
       61 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
      121 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
      181 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
      241 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
      301 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
      361 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
      421 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
      481 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
      541 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
      601 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
      661 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
      721 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
      781 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
      841 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
      901 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
      961 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
     1021 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
     1081 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
     1141 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
     1201 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
     1261 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
     1321 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
     1381 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
     1441 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
     1501 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
     1561 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
     1621 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
     1681 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
     1741 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
     1801 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
     1861 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
     1921 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
     1981 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
     2041 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
     2101 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
     2161 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
     2221 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
     2281 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
     2341 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
     2401 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
     2461 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
     2521 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
     2581 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
     2641 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
     2701 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
     2761 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
     2821 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
     2881 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
     2941 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
     3001 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
     3061 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
     3121 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
     3181 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
     3241 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
     3301 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
     3361 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
     3421 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
     3481 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
     3541 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
     3601 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
     3661 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
     3721 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
     3781 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
     3841 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
     3901 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
     3961 cgcagatcgt ttgcaaaaat agtttat
//
LOCUS       DAHUWM010000214         3981 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHUWM010000214 DAHUWM010000000
VERSION     DAHUWM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.219"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(91..1596)
                     /locus_tag="NK788_002483"
     CDS             complement(91..1596)
                     /locus_tag="NK788_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCH6770786.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1593..2279)
                     /locus_tag="NK788_002484"
     CDS             complement(1593..2279)
                     /locus_tag="NK788_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6770787.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2472..3893)
                     /gene="gndA"
                     /locus_tag="NK788_002485"
     CDS             complement(2472..3893)
                     /gene="gndA"
                     /locus_tag="NK788_002485"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCH6770788.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1096 a          848 c          684 g         1353 t
ORIGIN      
        1 tttataatga catccctgaa tttctcactt gcttgtattt tgtaccttat tctttgttgg
       61 atatataaaa aaacaagtaa agcacatgct ttacttgtct tctgcctttg cgataggaat
      121 aaagattcta aaaattgtcc cttgccctac tacactttct gctaagatac gtcctttata
      181 gttttcaacc agttgtttag cgatagacaa ccccagacca tttccaccct tcgttctagc
      241 tcttgcttta tccactcgat aaaaacgatt gaagatctta ttaaggtctt ctttgggtat
      301 tccttcgcca aagtcctgaa tcgcgatctc aaattcattc agagtggaag aaatagagat
      361 atggacttct tttcgtgtag ttgaatactt tactgcgtta tccaatatga taatgattaa
      421 ttgttcaaag tgattacgat aaatttgcag ggtcacttct tttgtcaaat catcatctaa
      481 agtgatcaca tagtctggat ataatatctt gaaattatta taaacttgat aggtgacttc
      541 ttttccttta gttgtttcat tcgcataatg aacatctact tgttctgctc ttgataagtc
      601 tagcatctct tgaactaagc ttttcatacg gctgatttct tgcatgctgg cgcttaatga
      661 ttcttctaat atctcaggat catctttccc ccaacgattt agcagactaa gatgcccttc
      721 aataattgca acaggtgtcc ttaactcatg agatacgtct tccacgaatt gctcttgttg
      781 ttcaatatac aaacgcatac ggtcaagcat ctcattgaat atttcagcta aatctgccaa
      841 ttcatcattt gtgtcgattt caggcatgtg gatatctgat tgcggatctt tacgtatagt
      901 atccattgta tctctgagta ccttgagtgg ttttaagaaa taagaagata aaaagaaacc
      961 taaaatacta cttaagatca aagaaacaac ttctaaaaca ataagagtca ataatagctt
     1021 atttcgaata tcatagaaag aagataattc atagaagctt tgcgcatagc cgatcttttc
     1081 tcttgtacct ttagaatata taggctggat agataaaaag ccagtttttc cttctagtgt
     1141 aacgatcgtt ggaactttac gtgtagtctg aatcaggttt cgactgtttt catgggtttt
     1201 aaaaatcaag ttctcgtcca aatcataaac ataaagatcc attgatggct gccctagctc
     1261 agctataaag gaatcgatct tcattaagtt tccttcgacc agtgaagtat gatctacgac
     1321 ctcatctccg tcatttgtcg ctttcgtcag attacgatac gtatttacta atgtcagttc
     1381 ttcatccgag ttagccaaac gagaagctac ttctgaaatc gtacgctcta cattgtttct
     1441 ttcttttgcc acaatcagat tgatagatga tttataggta atgacagcga aaattgtaaa
     1501 cacaacgaat atgaagaaag aactcgcaaa ggcccacttg attgtcaagg agggcccttt
     1561 cagttctctt ttttccgatt ttttcggctt tatcacgagc gcatcacata acctgttccg
     1621 cgtacagttt ggatatagct ttcctccccg ggaacgtcaa ttttgttgcg aagataacga
     1681 atatatacgt ccactacgtt tgtttcaact tctgtttcgt atccccacac tttatttagt
     1741 aaaacgtcac gtgctaatac cacgttcacg ttttccatta aagtaagcag caactcgtat
     1801 tctctttttg ttagttcaat tacttcattt ccgcgacgta cgacacggtt ttctttttcg
     1861 atagtcaaat cgcgataagt aatagtagtt tgtttagcca catttttatc gccttcaata
     1921 tcgattcgac gcaacaatgc acgaagacgt gctaataatt cttcgatagc aaatggtttg
     1981 acgatgtagt catctgctcc gtggtcaagt ccagatacac gatcgatcac agaatctctt
     2041 gcagtcatca taatgattgg tgtatttttg acttggcgta cacgtctgca gacttctaaa
     2101 ccgttcaatt ctgggagcat cagatccaac aatatggcat cccactcatt atttaatgcc
     2161 gcatctaaac cagttcggcc gttgtagtgg acttctgcgt tatacccttc atgcttcaac
     2221 tctagctcga caaatcgtgc caagttcttt tcatcttcaa tgattagaat attgctcatt
     2281 tattatttta tcctttctat tctactggct ttttaaaaac tctatcatta taccgataat
     2341 atgcttaaaa atctatagtc attttatcat ggatagcaat tttaaaaaag agctgaagtc
     2401 tactcataac aatcgacttc agctctttcg tcctcttttt attttctcat ttcctaacag
     2461 ccgaaagact attattcttc atggtaccat gagtaatgga agattccttc tttgtcgaca
     2521 cgctcatagg tatgtgcgcc aaagtaatcg cgttgggctt ggattatatt tgcaggcaga
     2581 cgttctgctc tgtatgaatc gaagtaagcg atggctgatg agaaagtcgg tacaggtacg
     2641 ccagcttgga cagccaaggc aacgacgtct ctgactgatt gctgatattt tttcgtgatt
     2701 tcaacgaagt aatcatctag caataaattt tcgatctcag gatttttgtc ataggcatcc
     2761 gtaatttttt gcaagaatcg agctcggatg atacatccag cgcgccagat ttttgcgatt
     2821 tcaccaaatg gcaagtccca atcaaattct tttgaagcga cacgtaattg agcaaatcct
     2881 tgggcataac tcatgatttt actgaaatac aacgcttctc ggattttttc gaccaattct
     2941 tttttgtctc ccgtgaactc aaaatcgttc gtacgagaca aaatcttgct tgcttgtacg
     3001 cgttcttctt tataagcaga aatatagcgg gcaaatacag attcagtgat caatggaagt
     3061 ggcacaccta agtcaagcgc gctttggctt gtccatttac ctgttccttt attcccagct
     3121 gcatcaagga tcacatctac tactggttta cctgttcctt catcgtcttt acgtgtcaga
     3181 atgtcagcag tgatttctat caaatagctg tctaattcgc cttgattcca ttctttaaag
     3241 atatcagcca tttcttctac tgaaaggttt aaaatattct tcattaagtc ataagattca
     3301 gcaatcagtt gcatatctcc atactcaata ccgttgtgta ccattttcac ataatgtccg
     3361 gctccatttg ggccaatata tgtgacacat ggtgctccgt cttctgcttt tgcagaaatt
     3421 tgctctaaga ttggtgcaac tagcgcatag gcttcttttt gtccaccagg catgattgaa
     3481 ggtcctttta aagctccttc ttcaccaccg gaaacgcctg taccgataaa gttgatgcca
     3541 gagttggcta actcttcatt acgacgcatc gtatctttaa agaatgtatt tcctccgtca
     3601 atcaaaatat cgcctttatc aaggtgagga agtaactctt ggattgttgc atctgttgcc
     3661 ggtcctgctt tgaccattag caagatccga cgtggtttct cgatagattc tacaaattct
     3721 tcgattgtat atgttgcttt gaaattttta tctgggtgtt cttctactac agcggttgtt
     3781 ttagcccctg tacgattgaa taaagccact gaatagccgc ggctttctat gttcaaggct
     3841 agatttttac ccataacggc cataccgaca acgccgattt gttgttttga catatgaact
     3901 cctcctacat acgggatttc tccctaatct cttacattat agcttaacac ggctacattg
     3961 caaactaaag aacaacatat t
//
LOCUS       DAHUWM010000215         3971 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHUWM010000215 DAHUWM010000000
VERSION     DAHUWM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.220"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(120..1376)
                     /locus_tag="NK788_002486"
     CDS             complement(120..1376)
                     /locus_tag="NK788_002486"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCH6770789.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(1488..1693)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1956..3257
                     /locus_tag="NK788_002487"
     CDS             1956..3257
                     /locus_tag="NK788_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCH6770790.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3326..3925)
                     /locus_tag="NK788_002488"
     CDS             complement(3326..3925)
                     /locus_tag="NK788_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6770791.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         1188 a          796 c          682 g         1305 t
ORIGIN      
        1 atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt
       61 ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact tactaaattc
      121 taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta
      181 ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta
      241 atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt
      301 gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt
      361 ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat
      421 gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact
      481 tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct
      541 aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg
      601 cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca
      661 agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat
      721 gggatcgtta aaccgaacgc ttctacttct ggtccttcta attttcggat taggtctaat
      781 ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt
      841 ttatgcagat gcaaaaagtc gatagattgc agaatttggt aagtaaactc tgtaaatgag
      901 attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag
      961 tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg
     1021 ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg
     1081 tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt
     1141 gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat
     1201 tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt
     1261 ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct
     1321 tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta
     1381 ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatgtgt aattaattat
     1441 ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa
     1501 tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac
     1561 gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta
     1621 ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga
     1681 tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac
     1741 agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc
     1801 gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat
     1861 ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga
     1921 caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt
     1981 taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc
     2041 gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag
     2101 aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg
     2161 attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac
     2221 aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta
     2281 ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact
     2341 ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga
     2401 tcgaacaact ttatcaaaat gattttgttt tagttaattt aaatgatttg atccaaaaag
     2461 acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg
     2521 ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc
     2581 gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag
     2641 aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt
     2701 tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt
     2761 atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag
     2821 ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg
     2881 gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc
     2941 agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag
     3001 acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc
     3061 aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta
     3121 cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag
     3181 acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag
     3241 cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa aaagccgaac tgacatttta
     3301 tcgtcagttt cggctttttt atgctttatt tcttcttcgg tgcgattccc ttgatcagga
     3361 cttctgtcca ttctgctgta tatggaagca gttttgcgta tatctccggc ttagaaattc
     3421 cttctaccat tgcttgaaaa tagaggatca agaactcatc cgaatatttc aaatccactt
     3481 ttccttcttt ccgccctcga tggaaaagat caagcatgat actgaaactt tcttgcgtat
     3541 acgcatccat cgcgtcagaa aattcttttt tttctttttt tgtatagtat gtcatcaaat
     3601 cgatataaaa ttgtttgttc actcgattga gataacggat tttgttttca ctcatggcaa
     3661 tcagtgtttc ttcaaatgga agattttccg caatcaacgc tttcgcttct tgccctaaat
     3721 cattcataaa tgcaaaaaat acttcataaa tcagcccttc cttgctgcca aaatatttaa
     3781 aaatcgtcgc tttacttacc tgagcgttct cagctacctt atccattgtc actccagcgg
     3841 ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc tttttctctt
     3901 gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg taattttaaa cttttataat
     3961 atatatagtt c
//
LOCUS       DAHUWM010000216         3968 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHUWM010000216 DAHUWM010000000
VERSION     DAHUWM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.221"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(176..1462)
                     /locus_tag="NK788_002489"
     CDS             complement(176..1462)
                     /locus_tag="NK788_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCH6770792.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="NK788_002490"
     CDS             complement(1455..2723)
                     /locus_tag="NK788_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCH6770793.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="NK788_002491"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="NK788_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCH6770794.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3496..3942)
                     /locus_tag="NK788_002492"
     CDS             complement(3496..3942)
                     /locus_tag="NK788_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770795.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1119 a          804 c          699 g         1346 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaaagaaag ccgattttca gtgtcggctt
     3481 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
     3541 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
     3601 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
     3661 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
     3721 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
     3781 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
     3841 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
     3901 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
     3961 ttttttat
//
LOCUS       DAHUWM010000217         3942 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHUWM010000217 DAHUWM010000000
VERSION     DAHUWM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.222"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            12..686
                     /locus_tag="NK788_002493"
     CDS             12..686
                     /locus_tag="NK788_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCH6770796.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            679..843
                     /locus_tag="NK788_002494"
     CDS             679..843
                     /locus_tag="NK788_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770797.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            919..2061
                     /gene="dcm"
                     /locus_tag="NK788_002495"
                     /pseudo
     CDS             919..2061
                     /gene="dcm"
                     /locus_tag="NK788_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2051..2341
                     /locus_tag="NK788_002496"
     CDS             2051..2341
                     /locus_tag="NK788_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770798.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2600..3784
                     /locus_tag="NK788_002497"
     CDS             2600..3784
                     /locus_tag="NK788_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCH6770799.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            3781..3918
                     /locus_tag="NK788_002498"
     CDS             3781..3918
                     /locus_tag="NK788_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCH6770800.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         1277 a          658 c          913 g         1094 t
ORIGIN      
        1 ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca aataaggata
       61 agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac gcagttttta
      121 tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct gagaattatg
      181 ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat tataatggaa
      241 aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca tttagatatg
      301 tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta agtgctgaaa
      361 aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat gtttatcaag
      421 accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga cgtcatgtgc
      481 cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca aaagtaaaga
      541 gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat aatatttccg
      601 aagttcatta tgctacggat aatctagaat tatttttacc actagggtat agtaggaaag
      661 atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt aaagaaatac
      721 aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa aacggtgatt
      781 aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt aaataaaaaa
      841 tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct tttttctttg
      901 caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg tggctttcgt
      961 ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga taaatttgct
     1021 agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca tgacatcaca
     1081 agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg cggcggattt
     1141 ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac tcgaggtact
     1201 ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt attccttgag
     1261 aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat cctccgaacg
     1321 ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc ctacattccc
     1381 caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac cgaacaagta
     1441 tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt ggtaaatatc
     1501 aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt agcgccaacc
     1561 ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat ctctgtagca
     1621 ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac tggaaccgcc
     1681 cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa tgaaacagaa
     1741 tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc cacaagtcgc
     1801 cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca acaagcgatc
     1861 gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg acttcagggc
     1921 tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca gttgtataag
     1981 caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag attaaaagag
     2041 ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga ttggaaagat
     2101 tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata ttcgcaagaa
     2161 tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg ctcgaattga
     2221 aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag aaccagatat
     2281 tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta cagatgagta
     2341 acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg aagcagaaat
     2401 cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt ctcacccccg
     2461 tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa gaatgcttgt
     2521 tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa gttaacacct
     2581 tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat taaaagaccg
     2641 acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac ttagtcggcc
     2701 gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca tgaggaaaat
     2761 ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt ttgactatgt
     2821 aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc tccatttgaa
     2881 atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac agtatgtcat
     2941 gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt tagaattaaa
     3001 ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta gctggtacga
     3061 ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact tggcaatcaa
     3121 tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga aggaagaatg
     3181 tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa aggctgatga
     3241 aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg tttatttctg
     3301 tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag accaaactga
     3361 gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg cgattcaaga
     3421 tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc gttacttgcg
     3481 atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg aacaatgggg
     3541 gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa aaccgtatac
     3601 cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga aaatggctaa
     3661 ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga atgcacgctt
     3721 atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa atttgattat
     3781 atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt tggtggcagt
     3841 atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga tcgagcaatg
     3901 gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DAHUWM010000218         3791 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHUWM010000218 DAHUWM010000000
VERSION     DAHUWM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.223"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            73..2172
                     /locus_tag="NK788_002499"
     CDS             73..2172
                     /locus_tag="NK788_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCH6770801.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2368..3231
                     /locus_tag="NK788_002500"
     CDS             2368..3231
                     /locus_tag="NK788_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCH6770802.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3336..>3791
                     /locus_tag="NK788_002501"
     CDS             3336..>3791
                     /locus_tag="NK788_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCH6770803.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1515 a          633 c          770 g          873 t
ORIGIN      
        1 attggcgtga cgtatagaat atgatagatt aaatggtagc gtaaacctag taaaggagcc
       61 gatcgctaag tgatgaaaaa atgtccaaat tgcggatcga aaaaaatgga gcagaatact
      121 atctgtccag actgcggtca tcaaataact gcagaagatc agcaaataaa agaatcaatt
      181 gaagaaacaa atacagatgc agctatgcag gataaatcaa ctgaagatgc agtctttcct
      241 gatacagaat taaacgatcc aatcgaatgg tcggaattga aagatttacc tctagaatct
      301 gtaatggaat tattcgaaaa agcagaacca gaagacgaag aaccacctgc tcacgatcaa
      361 gtaactaata tagagaaaaa tgaacagaag aaagcaactg ttcatgagac ttcggaagaa
      421 tcaggaaaga atcaagaaga aacgcttgaa tcaaaacaaa aaagaagagt agctgaactg
      481 aaagaaatcg tcgataatga ggaagaaaat tcgatactat ccgcttatat caaagcacat
      541 cgtgaagata caaaagaaga gcacgcgaaa gagctgctca aaatgatcag tgaaaaaatt
      601 gcacaggaaa acaaagtaga gccagaaaag gaacaagaaa ctgaaaaaga ctctgataga
      661 gaaaacgcca caggtaaaga aacagtaaaa ataaaagacg taaaaacgga tatcacagaa
      721 aaaactgaag tatttactga aaaacaatta gaaaaggaaa aaacgaataa actgccggaa
      781 gcagagaaaa aagaaatact cgaaaacaca gacaaaaagt caacagagcc cgatgaaata
      841 aatagtgaat ccgatgctga aaatcaatct gtaagcaaag agggtactga tcttgaagca
      901 aaaatagaaa aagaagagaa ccagcctaag ctgtttgaag cagaagcgaa atcaataaca
      961 atcgtgccag atactgaaaa aacacatcaa gaatcaaact tagaaaaaac agatccagat
     1021 aaagcgcaaa agaaaaaatc aaaaaaagta ccttatctcg ttttagcagc agtacttcta
     1081 ctcggtggag gaagctgggc ttattatgac catcatcaaa aagtagaagc cgaagtagca
     1141 gcagaagcaa aaagacagca acaaatggaa gaacttcatg agaaacttgc tgccttttat
     1201 acagatgatc accatcaatt tattcgtgca gatatgattc atcaagattt atcaaaacta
     1261 aaaaatgatt tatccagtct taagaatgaa aaaggatatt cagagttaga aacaacttac
     1321 caagatatcc aatctaagat ccgagatatc caaaaagtga atgagtggtt catagcacct
     1381 gccatcgtcg atgatcatct agcagatgac atcaagttga aagcagataa agagatccaa
     1441 gcaatcgaaa cggataacac agattttgga aaattgattc aacaagcaca aacagaagct
     1501 aaaaatcaat atgctgaatt gcaaacagct aaagaaaaaa cagctatagt ctttaaagac
     1561 ggaaaagtag tggattcagc aacacgcgac caatacaaga cggcaaaaga agcagtagaa
     1621 aaagtgaaaa atacagatct atctgctcag ttgaatgaac aattgaaaca agtagataag
     1681 atgctcactg ataaagagaa aaaagaagaa gaggaaaaga aaaaagcaga agaagcgaaa
     1741 aaagcagctg aagaacaagc aaaacaagcc gctcaagcag cagctgcagc tcaagccgct
     1801 caacaagctg cggtcgcaca agcagcacag caagaagcgg catcctcgac cccttcctct
     1861 tcaaactcag cgaatcagcc aatcaaatct gccagagcaa gtgatattgc cgatgcatct
     1921 aatccagcat ggacgtgggc accaggtgtc caaagcaaag tgattgctac atgtatccaa
     1981 cgtggatata tcgttgaagg aggttacaga ttagaaaaag ccagaatcga aaatggggaa
     2041 ggctattata atctatatgc cacttcaaca aaatctgcat tgatgaaagg cattggagaa
     2101 agtgctttgc cattttacat cgtaaccatc aactgtaaaa ctggatggtt cggaggaaat
     2161 gggagcaatt aattttaggc aaatgaaaga ggctgtgagc ctgccgttta acaccgttta
     2221 ttcgaaaaag gggagtatga cacaactgtt gtcatactcc ttttttcacc tttccaaacc
     2281 aaggctcagc tattcaagta gataacatta tgttatgata gcaaagaaat gtattaagaa
     2341 aacctttcac tatagaggga gaaaaaaatg aacgatttat tagcaactat atttacaggt
     2401 attattattg atgaaaatga aaaaaactac tttgtccaaa aaaacggaca aacctttggc
     2461 ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag ttgaaggatt cggttaccaa
     2521 aatcaaaaac atgaaaatcg gatcactaca caaattccta aaagccgtat cggtcattac
     2581 gcttttggaa tcgttactgc tacacgaaaa gatttaggtg cgtttgtcga tatcggtctt
     2641 ccagataaag actttgtcgt atctcttgat gaattgccga caatgacaga gctatggcct
     2701 aaaaaagggg atcgcttgat gatttccctt agagttgatg caaaagaccg tatttggggc
     2761 agtttagcag atgaaaaaat attcaaatct ttaagcaaat atggaacaga agaaatgaaa
     2821 aacaaagatg tgataggaac agcataccgc ttgaaattaa caggtacgta cgttttaacg
     2881 gaagatttct atcttgggtt tatccatcct tctgaacgat ataatgaacc tcgtctaggc
     2941 gagcagataa aaggtcgagt gattggtgtc cgaccagacg gtgtactgaa tctttcattg
     3001 aagccaagag catatgaagc tatatcagat gatgcacaaa tgattttaac cattttagaa
     3061 cgttctgcag atcagcaaat caactttaca gataaatcgg atccagaaga aatcatgaaa
     3121 gcttttggta tcagcaaagg agcatttaag cgcgcattag gtaatctttt gaaacaagga
     3181 ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa aatctaattg agaatccctt
     3241 gcatgttttt cttaactagc ttataataat tttaattaac atagtgttat atcttttttt
     3301 atttgtaatt attataaatt gttggggttg agtaaatggt catgaatgct atttcggcga
     3361 tgaaaaaaac aaaacagcag ctacatgaag cggggtttaa gctgacaccg caaagagaag
     3421 ctactctgct tgtgcttttg gaaaatgaga aagagcattt atctgcagaa gaaatcttct
     3481 ttttagtgaa aaaaaagagt ccggaaattg gacttgctac tgtttatcga acccttgaaa
     3541 tcttgacaga attgaaaatt gtcgataaag taagttttaa tgatggatta gcccgttatg
     3601 atttgagaaa agaaggcgcc aaacatttcc atcatcattt actatgcttg aactgcggca
     3661 cgatcgagga agtagaagag gatcttttac ttgaagtgga gcaagtagta gaagaacgtt
     3721 atcatttttt agtaaaagat catcggctaa cgttccatgg aatctgtcag gaatgctatg
     3781 aaaaagaaaa a
//
LOCUS       DAHUWM010000219         3745 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHUWM010000219 DAHUWM010000000
VERSION     DAHUWM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.224"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(456..881)
                     /locus_tag="NK788_002502"
     CDS             complement(456..881)
                     /locus_tag="NK788_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770804.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="NK788_002503"
     CDS             1255..2226
                     /locus_tag="NK788_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6770805.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="NK788_002504"
     CDS             2258..3655
                     /locus_tag="NK788_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6770806.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1288 a          605 c          771 g         1081 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aactt
//
LOCUS       DAHUWM010000220         3720 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHUWM010000220 DAHUWM010000000
VERSION     DAHUWM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.225"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            272..1159
                     /locus_tag="NK788_002505"
     CDS             272..1159
                     /locus_tag="NK788_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCH6770807.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            1156..2325
                     /locus_tag="NK788_002506"
     CDS             1156..2325
                     /locus_tag="NK788_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770808.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2349..3704
                     /locus_tag="NK788_002507"
     CDS             2349..3704
                     /locus_tag="NK788_002507"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCH6770809.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         1230 a          672 c          870 g          948 t
ORIGIN      
        1 aaagaagagc tgctagaaga gctgccagag cagaacgacg taaaaaaatg gaacaagcta
       61 caaaagaaaa aaaggaaaaa taacagaaaa aatataatga ataattctct gaagtttaaa
      121 aatatttatt gcagagaaaa ataaaacgca tacttgcaat tttcttgaaa attatgcgtt
      181 tttctcaaga aaaatacgag catatcagcc tctaaaatta aaacatatga tatactagag
      241 aggaaatctc aaaagagaag agaggctttt tatgtcattt caaatcgggg aactttttaa
      301 tttggactat tggcagcaaa tgttttccgg tgacttgttc tctctgaatt ttttggtgaa
      361 catacttgat attctggttg tctggtatct tgtatacaaa ctgatccaac tgctgcgagg
      421 cacaaaagcg attcagcttc tgaaaggtgt agctattttt attgttatcc gaatcgtagc
      481 ggaaatcatt ggtctgcaca ccttgtcttg gctgatgaac caagtcatta cgtacggggt
      541 gatagctgct atcgtgattt ttcagccgga aatccggaga ggactggaac atcttggacg
      601 cagttcgctt ttcaaacaat caaaaagcga acagcaggaa gatgagaaga tgatcttgtc
      661 ttttgacaaa gccatccaat atatgtccaa gcgaaaaatc ggtgcgttga tcactatcga
      721 acgtcatact ggattagatg agtatattga gacagggatt gctttagatg cagatatcac
      781 tggggagctg ctgatcaata tctttattcc taatacaccg ctgcatgacg gtgcagttat
      841 tgtaaaagaa ggaaagatcg cagtagcaag cgcctatctt cctctatcag aaagtatgtt
      901 gatccctaaa gaattcggga ctcgacaccg ggcagctgtc ggtataagcg aagtcagtga
      961 cgcgattacc atcgttgtct ctgaagaaac cggggacgtc agcatcacgt tagataatga
     1021 actgatggct ggcttatccc agcaagaata tttggcaatc ttgcgtagag aactaattgc
     1081 tgaagagaaa acaaaaaaca agaaaaacgt gattcaatct ttcctagacg gtatgactaa
     1141 gggaggggat cgcaaatgat ctcaaaagag aaacggacaa gcattcttta cagttttatt
     1201 gcattgctgt tcagtattgc gctgtacttc aatgcaaatg gccagtccgt tccaaatacg
     1261 ctttctggaa atgaagcgta taatcaaaca gttacggggg tgccgatcaa gatttcttat
     1321 gattcaaagc gctactatat ccatggatat gaaaatgcag tgactgtcaa actttccagt
     1381 gcaaaccgag ttcagttgaa tgcagaggca aacgaagata cacggatgtt ccgcgtcaca
     1441 gcagatatca ctaagctggg cgaaggaaca catgaagttc cgctaaaaat tcaaaatttg
     1501 agcagtgctg tcacagcaaa gatcgaacca tcgactatta cagtaacggt tgagaaaaag
     1561 gtaataaaag atttcgatgt ggaaacgttg attccttcaa cgttcacacc atctggatat
     1621 gaacttgatg atacttccgt tagtcctaaa aaagtctcta tcactacagg tgacaaaaca
     1681 ttggcagaaa tcaaacgagt agtagctgat gtggacgctt cgatggtcac agatgatggg
     1741 attaatagtg aggtcccaat acaggcgctg aatgcagcag gggaagtatt aagtatcgtc
     1801 tctgatccag tgcaagtaac agtgagagct gattcgatca agccaagcaa gtcggtacga
     1861 ctttatggga tacagcaagg aacaccagct gccggggtag aaagctatga tttcagtttt
     1921 tcaaaactgg aagcagaagt aagcggctct agcgatttgc ttgcatcgat cggtgacagt
     1981 attgctgtac cgatcaatgt ctcaggtatc aatcatcgaa caactcgtaa aattgaaata
     2041 ccagtagaag aggggttaag tatcaagccc gaatcagtta gtgtggagat tacacccgtg
     2101 ttgcaaacgt caagcagtag ctctgcttcg acgagcagtc ggacacaaga aaatacaagt
     2161 acgagtagaa gtatttcatc tgctgattca ggaacggaac catcgacaac aaccatttct
     2221 tccagttcgg aatctgggac aagcagctcg accacaagta cagaagaaac atcagaaaca
     2281 agcgaatcaa cacaaatgag cagaacaaat tcctcagaaa attaagttaa aggagtaatt
     2341 ttacaactat gggtaaatat tttggaacag acggcgtacg cggtgaagcc aacaaggaac
     2401 ttacgccaga acttgcattt aagctgggac ggtgcggagg ttatgtccta agccagcatg
     2461 aagaagaagg aaaacgccca cgcgtgttag tagggcggga tacaagaatc tcaggtcaaa
     2521 tgttagaaga agcgctgatt tcaggacttt tatcggtggg tatcgaagtg ttccagctag
     2581 gtgtgatctc tacaccaggt gttgcttatc tgacacgttt gcaaaaagca agcgcagggg
     2641 tcatgatttc tgcttctcat aatccagcac aagataatgg aatcaaattt ttcggtgcag
     2701 atggttttaa actagtagat gaccaagagc tagaaattga agcattatta gatgctgaag
     2761 tggatgaatt gcctcgtcca tcagctgaag gattgggaac tgtagaagaa tttccagaag
     2821 gcttattgaa atattctcaa ttccttgtac aaacgatcaa cggtgacctt tccggtttga
     2881 ccatttgtgt cgatgcagca aacggagcaa cttcaacaag tgttaatcgt ttgtttgccg
     2941 atcttgaaac agatttttat acaatgggca caaatccaga cgggttaaat atcaatgacg
     3001 gcgtaggctc tacccatcca gaacgtttag ctgagatggt cgttgaaaaa ggagcagatg
     3061 ctggactagc tttcgatggc gatggcgatc gtattattgc ggtggatgaa cttggaaaca
     3121 ttgtcgacgg tgataaaatc atgtttatct gtgctaaata cttggcagaa aaaaatcgat
     3181 tgaaaaaaga tacgatcgtt acgactgtca tgagtaactt aggattccac aaagcggtgg
     3241 aaggaatcgg tttgaaagat gtcgttacac aagttggtga tcgttacgtt gtagaagaaa
     3301 tgcgcaaaaa cgattataac tttggcggcg agcagtctgg gcacatgatt ttcttagatt
     3361 ttaacacaac tggagatggg atgctttcag gaattcagtt actgaatatc atgaaacaga
     3421 caggtaagaa attatcagag cttgcagcag aggtaacaat ctatccgcaa aaattagtga
     3481 atatccgcgt aagtgataaa aacggtgcga tggatgttcc ggcaatcaaa aaagtgattg
     3541 aagaggcaga agctgaaatg ggatcagaag gaagaatcct agttcgtcct tccggtacag
     3601 aaccattact tcgagtgatg gcagaagcgc ctactgatga gaaagttaat tactatgtcg
     3661 ataaaatcgc ggcagtagtc aaagaagaaa tcggactgga ttaagagata ttttcataaa
//
LOCUS       DAHUWM010000221         3684 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHUWM010000221 DAHUWM010000000
VERSION     DAHUWM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.226"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            14..793
                     /locus_tag="NK788_002508"
     CDS             14..793
                     /locus_tag="NK788_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770810.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            839..1783
                     /locus_tag="NK788_002509"
     CDS             839..1783
                     /locus_tag="NK788_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCH6770811.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1799..2578
                     /locus_tag="NK788_002510"
     CDS             1799..2578
                     /locus_tag="NK788_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCH6770812.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2590..3288
                     /locus_tag="NK788_002511"
     CDS             2590..3288
                     /locus_tag="NK788_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCH6770813.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3316..>3684
                     /locus_tag="NK788_002512"
     CDS             3316..>3684
                     /locus_tag="NK788_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCH6770814.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLF"
BASE COUNT         1312 a          699 c          780 g          893 t
ORIGIN      
        1 taggagtgaa tctatggaaa agccattcgt caagtcattg actgctgtgg cggtaggtgc
       61 aggtgctatt gcgatctgtt tgtttggata ccatatcaat aatcagcggc aacatcagca
      121 acggatcaat tatgcagaat ctgccatcac caatcaaaaa gacaccttga cttctttatc
      181 aaaagaagta gacaagctgt atagtacaaa agaaaaaatt tttctaaacc ctgaaataac
      241 agaagaaaca atcacaagtt tgtcaaataa gctatcctct gtcaaacttt cagcagatga
      301 ttacgacatc aaagaatcag aattgcctaa agaagctgtt gcaatccaag atgagaagaa
      361 agcaatcgtg acacagttag atagtgcaga atcaaaactg aagatccaga cagctgtcaa
      421 cgatttattt actaaaaatg tatcaaactg gcagcaagct gtagacgatg tcatcatcaa
      481 agaaaaggtg acaagtgcag atgtcgctcg tgtacgtgaa aatatgagct tttttaaaga
      541 tagtccttgg aaaacggttg ttatgcaata tctaggattc gctgatattc agatcgcgca
      601 agtcacgcaa ctagatcagt tattcgatac gatgttaaag gatggtcaag tgactgccac
      661 cgctacttac gatcaatatt tgacggtgct cagtcaaata gagcagatcc ggaatgaaaa
      721 aataagcgca gcatacgcaa caaaagcaca aactgttgct gagcaaatgg gctatagcaa
      781 tataagctat tgacagttaa aacgttgatt caagcaaggt tagcaaacgg aggaaactat
      841 ggcaagacga agaagaaaat caagagggaa aaaaatattt ctgattattt tagcagttct
      901 tatcgtatta gtattagcaa ttgtaggagt aggagcaaaa ttatatctgg atctctcaca
      961 ttctattgag aaaacttacg aatcagtgga acgaacccaa ggagaacgaa cgaaaccagt
     1021 tgatctggac aaacaagaat ctttctctgt tctattgatg gggattgata ctggagattt
     1081 aggtcgggtc gatcaaggaa gatcagatac gatgatggtt gccacagtca gtcctgaaga
     1141 caaacagaca acgatcgtca gcattcctcg tgatacgtat gtcgacatcg taggtcgtga
     1201 cacaaaagac aagatcaacc acgcttacgc attcggcgga gctgccatgg cgatggatac
     1261 cgtacaaaaa tatgtagaca tcccaatcga tcactatgtc tctatcaata tgaaagggct
     1321 aaaggaactc gttgatgcag ttggtggtat cgaagtcaat aatgacctca ctttctcaca
     1381 agatggctat gactttccaa tcggtaaaat tacattgaac ggggatcagg cattatctta
     1441 ttctcgtatg cgttatgaag acccaaatgg tgattacggt agacaagggc gccaaagaaa
     1501 gatcgtagaa ggcgtagcga aaaaagtatt gagtctaaac ggtgtcagca actatcaatc
     1561 cgttttgaat gctctagagt cgaatatgaa aacagacatg aactttgatg aaatgaaaaa
     1621 aattgcttta gattatcggg aggcatttgg tacgattaag caagatcagc tgcaaggaga
     1681 aggcttcatg caagataata tctcctatca aaaagtcagt gatcaagaac ttgatcgtgt
     1741 gaaaaaagaa ttaaaagatc aaatgaatct agagaataaa taggaaagtg gagaaaaaat
     1801 ggaagagaca atcagcttga aagaaatttt tgaaacatta cgcaaacact tggtaataat
     1861 catcatctca atgtttgctg gcttggccat ctcaggaatc gtaacatttt tcatcttgac
     1921 accaaaatac agttcacaag cacaattgat cgtgacactg ccacaatcag acactgcaaa
     1981 tgtcaatgat gtcaatacga acctgcaaat gatcaatacg tataaagata tgatcgtcag
     2041 tgatttagtg ctcaatgaag tcaaagatcg tttagaaaca gaagacaatg tgaaaatgac
     2101 tgctgaacaa atcaaagatg ccatctcagt taatcaggca gaaaattcac aaatgttctc
     2161 gattcaggca ataagtacga atgcagtggc tgctcaacag atcgctaaca caacttcaca
     2221 agttttccaa gagaacgcaa aagatgtgat gaatgttgat aaaatctcga tcatttcagg
     2281 tgcagttgcc aatacgacac ctgtttcgcc aaataataaa ttgaatttaa tgactggctt
     2341 aatgttagga atgatggtag gtgtaggtct ggcattctta ttggaattac tcgatcggac
     2401 agtgaaagat gaaaaatatg tgacagatac attaggattc ccaatcttag gaacagttcc
     2461 agaaatgtca gcgaaagaat tgaatgctgg tatccgtaaa aaaggaccag ctcagacaca
     2521 accaaaagct acaaacgata aaacagcgcc atcacgcaga agccgctcaa gagtctagga
     2581 ggaaagaaaa tggcacgaac acagaaacaa aaagcagccc aaactcatgc agtcagtttg
     2641 gtcacactca ccaatccctc ttcaccaatc gctgaacaat atcgtacgat tcggacgaat
     2701 atccagtttg cctccgcagc tggtcagcaa atcaaaacga ttgttgtgac ttcatcagga
     2761 cctggagaag ggaaatcgac cactgcagca aacattgccg ttgtttttgc caaatctggt
     2821 caacgggtgc tgctagtcga tgcagactta cgtaagcccg tcatctataa aacgttcaag
     2881 ttgaacaacg caagcggatt aagtactgtt cttagttcat caggaagtgt agcagatgag
     2941 atccaacgaa caccagtcga caatttgtcg attctgccaa gtggacctaa accaccaaat
     3001 ccatcagaac tactaagttc gccaagaatg gatcagattt tagcagaagc aagacaacta
     3061 tttgatgtag tgatcttcga catgccgcca gtcgttgccg taacagatgc acagatcatg
     3121 tcttcaaaaa cggatggcac cttattggtc gtacgggaaa atacgtcacg aaaagaatca
     3181 ttgactaaag caaaagaact gttagatatg gtacaagcac gagtcttagg tgtcgtatac
     3241 aacggagcag aacacagcaa agatgctggt tattactacg gaaattaagg aataagaaga
     3301 gaggagacaa gagaaatgat cgatctacat tgccatatcc taccaggaat cgatgacggg
     3361 gcagaaaact tagaagcaag tattgcaatg gctgaaaaag cgattcagca aggcattacg
     3421 catattttat gcacaccaca ccacaataat ggaaaataca gcaatgaaaa atcacaagtc
     3481 atttctctag tcgcttcgtt acaagccgaa ttagagaaaa gacagttgcc tttgacgtta
     3541 ttggaaggac aagaagtacg tattaccgga acgctcatcg aagacatcca ccgagatgaa
     3601 attttattta cagatttaga tgatacatat cttctgatcg aatttccgac actggaagtg
     3661 ccgttatatg cagaacggct tttt
//
LOCUS       DAHUWM010000222         3648 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHUWM010000222 DAHUWM010000000
VERSION     DAHUWM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.227"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..965)
                     /locus_tag="NK788_002513"
     CDS             complement(<1..965)
                     /locus_tag="NK788_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HCH6770815.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLSEKIIENFKEITPFSSFKL"
     gene            complement(969..1379)
                     /locus_tag="NK788_002514"
     CDS             complement(969..1379)
                     /locus_tag="NK788_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6770816.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1397..1888)
                     /locus_tag="NK788_002515"
     CDS             complement(1397..1888)
                     /locus_tag="NK788_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6770817.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1898..2689)
                     /locus_tag="NK788_002516"
     CDS             complement(1898..2689)
                     /locus_tag="NK788_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6770818.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2682..3455)
                     /locus_tag="NK788_002517"
     CDS             complement(2682..3455)
                     /locus_tag="NK788_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770819.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1136 a          766 c          498 g         1248 t
ORIGIN      
        1 tctaacttaa aacttgaaaa tggagtgatt tctttaaaat tctcaattat tttttcagac
       61 agtttaacac caagagtttt gtctgtcttt tgaataattt gatgtggaac aagtgcaccg
      121 ccccaatccc agttattttg atcgttctcc ccttcttttg ttgccaccca gccagttaga
      181 aaacggccag tttcattagt tccgactgtt cttgctgcat aataggcttt tccgtcgaaa
      241 gcttcgtcta ctggtgattt ccaatctgaa aataatcctg ctccgactcg atatttagtc
      301 tttttatctt cacaatactc tgaaaaaagt aaataccacg ttccatcaat ttcaaatata
      361 tcaggcattt caatcatgtt aaattcatta ttagcccagt agtctccttt gaaattccaa
      421 tcttttaaat cttcggattc aaaatagacc aatctccccg tcggcttttt cttacttgct
      481 ggttttctag ctcctagtat caaaacgtat tttccatgat cttgatcaaa aattgcaaaa
      541 ggatcgcgcc aatcattctt atcatatcct tcttgaggaa caagttttgc tgcttcgcca
      601 tcctttttcc aatgaattaa atctttagaa cttgcaatca ttaacacttc tgaagttttt
      661 ccatccaact tagctttgcg attatgtcca gtatacaaag ctaaatactc attgattcct
      721 tggacaatac ttccagcgta aatgtattga tcaatgtcat cgttgccacc acgatgaatt
      781 acttccccgt attcttcaaa tttcacaaaa tcagtggttg taactagtga ccacccaaaa
      841 ggatcagtta aaggtttagg atttctcgta tctcgttgat gaaatagata aaaagttcca
      901 tctttataaa aaggcatgca atccccaaac caaaaatctt taggcttgta aaaaattttt
      961 tgcatttatc actcatcctc ttcgttagta tgtctttgca tatctgcaag acacgcaatt
     1021 gtattcatat ttcttagatt ttcgatgata tcatcgcttt tttttccttg taatctcaaa
     1081 ttgacagcct caatcaaaat tcctaaattc atgccaccaa gaagttgaaa cccattttga
     1141 aatacttcac ttcttttagc aaattcgttg aaaggtgttc caccaatcaa gtcagcaaat
     1201 accataactt cttgattttt tacttgagaa actatttttt caatgttttc acggaattct
     1261 tcaatgcctg catctccaag tgttaccaca tgacaattta aattatcgcc aaaaaacatt
     1321 tttaatgtat ctacaaatcc aatggcaata tttccatgag atgtaattat aaactccatc
     1381 cactcatcct ctgttcttaa taaataaaat cctttaaaaa ttcttttgaa tctctaggag
     1441 tttgtatcac tgtcacttca actccatcac taattagttc tttaaaagcc tctctctctt
     1501 cttcagtaat atttatcgtc ggacggatgc gttctgttcc attccttctt gataaatttc
     1561 ctacgtttac acttgttatt ggtaaaccgt tatcaatcaa aaatttaaca tcgattggag
     1621 aaactgcaac aataagaact ttttgtcctt catatttacc agaaagaata tttttaactg
     1681 ctacttccct tgtaattatt gaagttctaa cattaggtgg tgcagcgagg cgaactacag
     1741 ctttttttac gtcatcattg gctacttcat tattaataac cataattctg ttagcactca
     1801 attgatttgt ccattgtgtt gctacttgtc catgaatcat tcgatcatca atacgaacat
     1861 gtataatacc tttcattttt tcatccatat tcaacaccta cttcaaaata tttaacgctc
     1921 ttaacgcaat agaaactact aagattaata gaattacata aactggttta acctttcgtc
     1981 ctaacaaata atacacaatt gctactacac atgcaggtaa taaaccaggc ataacttgat
     2041 caaagatttc atttccggtc attgttaagt cgccttgttt aaattcaaag ccaacattca
     2101 cttttacaac tgtggcaatg agtgcaccga ccaccattaa ccctaaaatt gatactgcat
     2161 cagtaataga ctttaatttt gtttcaattt gagttagtag gctcgcaccc gattctttcc
     2221 cagcaaggta taaaggataa gcagtaatcc ttaataaaat tgatgccagt atccacaaaa
     2281 taattccaac aaaactacct tccaatgcca tattagctgc aattgctcca aaaatagtcc
     2341 atggaataac tacaaataca ctatctccaa ttcccgctaa aggacccatt agacctgttt
     2401 tcagagcact tactgcttcc aaagacttat ttttttcttc tttttcaatt ccaacattca
     2461 taccaataat gtaaggcgct gtatacggcg tagtgttaaa aaattgtaag tgagctaaaa
     2521 cggattgttt cattccttca ggatcatctt tatatgtgtc cttgataaaa ggaagcatag
     2581 aatacgcata acccaaccct tccatttttt cataatttaa tgaagttgca ccattgagaa
     2641 tccaccggaa aattacttgt ttgtcgattt ttgtcagttt cttattcatc ttcgtcaaaa
     2701 cctcctgttg cagctggtgc tacttgagta ttatttgatc tatttttata tacgattagt
     2761 gcgattgcag cgccaagtaa cgaaatccct aaaattggta tttggaaata aactgccaaa
     2821 acgaaaccaa ctaacaaata ggcaaagtaa tcttttgtag gtaaatattt aagtagcatt
     2881 cctatcccaa cagcaggtaa tagtccccct gctaccttta aaccaccggt aacccattca
     2941 ggaatataat tgattgcact tcccactaac gttgggccta atatcacagt taacaaaaca
     3001 ggaattccag atacaaagct tgtattaatt gatgccaaat attgcatcca ctctaatttt
     3061 tgataatctt ctttttgtgc gaatttttct gccttttgtt gaaaaaaaat gtttgttgac
     3121 cactttagaa catctagttg tacaattaac attgctaccg gtattgctaa agttattcct
     3181 atagaagcat cctggttagt tgagattgct aaataagtac cgatcaatgc tgctgtttga
     3241 taatccggta ctgatgcacc accaaaacta gtaataccta gtgtcattaa ttgcaaagtc
     3301 ccaccgatat aaagtccagt ttgaatatct cccataataa gtccggtcaa gaaacctgca
     3361 acgacaggtt gggaggttcc gattttgggc ccttccttat cccagttaat gaaaaatcca
     3421 tacaacacaa ctaaaaatgt ttgccaaaac gccattttgt tctctccttt cttcttatac
     3481 ttttatataa gcaattactg tgccaactaa cattatccaa taaagcggga aaatacagta
     3541 aatattaatt aaatgagcgt ctaaatatac tttggacacc ctttcatttt tttagacgct
     3601 agtatgctat ttagaaaata gtctgctcaa aaattgtata gattaact
//
LOCUS       DAHUWM010000223         3605 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHUWM010000223 DAHUWM010000000
VERSION     DAHUWM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.228"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            127..579
                     /locus_tag="NK788_002518"
     CDS             127..579
                     /locus_tag="NK788_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770820.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="NK788_002519"
     CDS             593..1282
                     /locus_tag="NK788_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770821.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3605
                     /locus_tag="NK788_002520"
     CDS             1407..>3605
                     /locus_tag="NK788_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCH6770822.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGAYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGV"
BASE COUNT         1218 a          480 c          750 g         1157 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggg cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgta
//
LOCUS       DAHUWM010000224         3602 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHUWM010000224 DAHUWM010000000
VERSION     DAHUWM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.229"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            75..470
                     /locus_tag="NK788_002521"
                     /pseudo
     CDS             75..470
                     /locus_tag="NK788_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="NK788_002522"
     CDS             complement(465..683)
                     /locus_tag="NK788_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770823.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="NK788_002523"
     CDS             790..1104
                     /locus_tag="NK788_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6770824.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="NK788_002524"
     CDS             1208..2386
                     /locus_tag="NK788_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6770825.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="NK788_002525"
     CDS             2789..3082
                     /locus_tag="NK788_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCH6770826.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1225 a          528 c          709 g         1140 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 ct
//
LOCUS       DAHUWM010000225         3602 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHUWM010000225 DAHUWM010000000
VERSION     DAHUWM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.230"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..401
                     /locus_tag="NK788_002526"
     CDS             <1..401
                     /locus_tag="NK788_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCH6770827.1"
                     /translation="FFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCLAQLTAP
                     SYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFGKEKVVQ
                     MKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            445..1896
                     /locus_tag="NK788_002527"
     CDS             445..1896
                     /locus_tag="NK788_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCH6770828.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            1928..3064
                     /locus_tag="NK788_002528"
     CDS             1928..3064
                     /locus_tag="NK788_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCH6770829.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            3064..>3602
                     /locus_tag="NK788_002529"
     CDS             3064..>3602
                     /locus_tag="NK788_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCH6770830.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIR"
BASE COUNT         1265 a          440 c          787 g         1110 t
ORIGIN      
        1 ttttttttga gttgttagaa ctaggaaaga taccagtgat cgtccatcca gaacgcaacg
       61 cgcattttcg agaagaccca aatcatttgc ttccgttcct agatatggga tgtttggccc
      121 aacttactgc gccaagttat gttggtgtat ttggcaaaga tatccagaaa acagccaaaa
      181 caatggtcaa acataatctc gtgcagatgg ctgcttctga tgcacacggg gtctctaagc
      241 gcacctttta cttaaaagag tgctatgagc agattgccaa ggagtttggt aaggaaaaag
      301 tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg ggatgagatc aattatccaa
      361 tctatgaaga ggtaaaaaag aagaagtttg gtttatttta gttctttatt cgtagataaa
      421 aaggaaatga ggagagagtg agagatgtat agaaaaaact caagtggttg gtctaaacat
      481 attgatttta ttttattaga cttattttgt gtccagcttg ctttttatat atcatatgta
      541 atgcgacagg gtgactggaa tccgtatgtg attcctattt atcgccacat ggcaattttt
      601 atagaacttg agaatcttac gattatattt ttatttgagg catataaaaa tgttttaaaa
      661 agaggatatt ataaagaggc tgtagcttct ataaagcaat cttttatgct gttacttgtg
      721 tgcagtttat accttattac aatgcaggat ggaaatgatt attccagagt ttctttattt
      781 ataatggggg tggtgtatgg attattaaca tatataattc gtatactttg gaaagggttg
      841 ttgcatcacc gcatgaaaaa cggtgaagat gtatcgttag ttattgtaac atctcaaaag
      901 attgcaaaaa atgtagttcg taatcttaaa gagaagaatt atggcatgta taaacttagt
      961 ggtgtgattg tagtagacag agatatgtat ggaactacca tcggaggtgt cccagtagtt
     1021 gcaaatgctg ataatgcagc ggaatatctt ttacagaatt gggtagatga agtattcatc
     1081 aatgttgacg aaagtgttcc atatcccaaa gaattaatcg ccagatgttc ggaaatggga
     1141 cttgctgtac atatgaatct tacaaaagtg tctgatagta cacgtggaaa acaatttatt
     1201 ggccgagttg gtgattatac tgttattaca tctagtatta atgttatgac gatgaggcaa
     1261 gcatttataa agagaacgat tgacattctt gctggaatta ttggatgtat tgcaacaggt
     1321 gttattttta tcttcgtagc accagttatt tacatcaaat caccagggcc gattttcttt
     1381 gcacaagaac gtattggtca aaatggtaag acatttaaga tgtataagtt ccgtagtatg
     1441 tatatggatg cagaagaacg caaagcagag ttgatgaaag aaaataagat gagtaataat
     1501 ctcatgttta agatggattt tgacccaaga atcattggca ataaaatact tcctgatgga
     1561 acaaagaaga caggaattgg acaatttatt cgatctacat cattggatga gtttccacaa
     1621 ttttataatg tcctaatggg aaaaatgagc ctggtggggt ttagaccttg cttgaagtca
     1681 gaatatgaga agtatgattt tcatcatcgt gcgagaattg cgatgaaacc tgggattact
     1741 ggaatgtggc aggtgagtgg tagaagtgat atcacagact tcgaagaggt tgtaaaactt
     1801 gatgcagaat atatcaggaa ttggagtatg ggattagatt ttagaatttt gtttaggaca
     1861 gtcaaaactg tgcttcatag aaacggttcc atgtaagata aagatactat agaaggagta
     1921 aaacgtgatg gcaagaaaaa aattaaaaat ctgtatgcta ggtcataaaa gaattccatc
     1981 aagagaaggt ggaattgaga tagtggtaga agaattggca acgcgaatgg ttgcacttgg
     2041 gcatgaagtg acttgctata atcgaagtgg tcatcatgtt agcggaaagg aatttgacca
     2101 gcataaaaat aaagaatata aaggtgtaaa attaaagact atttttacat taaatataaa
     2161 aggaattgca gcaatgagtt cttcagtatt tggtggaatt agagcagcat tcggaaaata
     2221 tgatgttgtt cattttcatg cagaaggacc atgtgctatg ttgtggttgc caaaactttt
     2281 tgggaaaaga tgtgttgcaa caattcatgg tctagatcat caaagagaaa aatggaacaa
     2341 gcttgctagt acatacatca tgttagggga aaagtgtgct gtaaaatttg cagatgaaat
     2401 aattgtactt agtgaaagcg tccaaaatta ttttgaagat atatatggaa gaaaaacaag
     2461 gtttatacca aatggcgtta aaaaaattga aataaaatca gcaggattga ttacagaaaa
     2521 gtatggatta acaaaagatt cttatatttt gtttttgggg cgattggttc ccgaaaaagg
     2581 aattcggtat ctaattgagg catttaaaga tgttcagaca gataaaaaac ttataatagc
     2641 cggaggaagc tctgatacag atgaatttgc aaatgaacta aaggaattag caaaaggtga
     2701 tgagcgaatt atatttactg gatttgtaca aggacaggaa ttagaagaat tatatagtaa
     2761 tgcgtatatc tatacacttc caagtgattt agaaggaatg ccattaagtt tgttagaagc
     2821 aatgagttat gggaactgtt gtatagtttc aaatatctca gagtgtacgg aagtagtaga
     2881 agacaaggct atgattttta agaaatcgga tgtaagtgat ttaaagataa gattagaaga
     2941 ggcatgcaat caatcggaaa tggttaaagt tttgaagaat caagctactg agtttatttg
     3001 tagtaaatat aattgggata aaatcgtaca agaaacatta aatttgtata ggggaaatat
     3061 ataatgattc gaatattaca gtgtgtgaat gatatgcatc gtgcagggtt ggagacgatg
     3121 ctaatgaact attataggaa tatagatcga actaaaattc agtttgattt tttgacacat
     3181 cgtccatata aaagtgatta tgatgaggaa ataataagtc ttggaggaaa ggtctattat
     3241 gcaccgagac tttatccaca aaattatcct aaatatttta aatggatgga acaatttttt
     3301 aaggatcatc cagaatatga gattgttcat tctcatattg atgcgatgag ttatttacct
     3361 ttaaaagctg caaaaaaagc tgggattccg atacggatag cacatagcca taatacatcg
     3421 atagatagag attttaaata tcttttaaag caatattatc gatgtaagtt aacgtctgtt
     3481 ttgacacatg aattttcatg tggaaaagta gcaggtgatt ttttgtttcg aaatgataaa
     3541 ttcactttga ttccaaatgc agtggatgct aaaaaatttt attatgatga aagtattaga
     3601 aa
//
LOCUS       DAHUWM010000226         3566 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHUWM010000226 DAHUWM010000000
VERSION     DAHUWM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.231"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            69..1358
                     /locus_tag="NK788_002530"
     CDS             69..1358
                     /locus_tag="NK788_002530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCH6770831.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1605..2855
                     /gene="clpX"
                     /locus_tag="NK788_002531"
     CDS             1605..2855
                     /gene="clpX"
                     /locus_tag="NK788_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCH6770832.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            2938..3525
                     /locus_tag="NK788_002532"
     CDS             2938..3525
                     /locus_tag="NK788_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCH6770833.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         1288 a          596 c          763 g          919 t
ORIGIN      
        1 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
       61 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
      121 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
      181 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
      241 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
      301 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
      361 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
      421 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
      481 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
      541 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
      601 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
      661 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
      721 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
      781 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
      841 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
      901 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
      961 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
     1021 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
     1081 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
     1141 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
     1201 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
     1261 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
     1321 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
     1381 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
     1441 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
     1501 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
     1561 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
     1621 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
     1681 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
     1741 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
     1801 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
     1861 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
     1921 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
     1981 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
     2041 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
     2101 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
     2161 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
     2221 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
     2281 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
     2341 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
     2401 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
     2461 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
     2521 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
     2581 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
     2641 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
     2701 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
     2761 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
     2821 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
     2881 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
     2941 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
     3001 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
     3061 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
     3121 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
     3181 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
     3241 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
     3301 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
     3361 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
     3421 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
     3481 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
     3541 cttaccagtc aaactctttc ataaaa
//
LOCUS       DAHUWM010000227         3459 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHUWM010000227 DAHUWM010000000
VERSION     DAHUWM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.232"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            33..1772
                     /gene="yfcC"
                     /locus_tag="NK788_002533"
     CDS             33..1772
                     /gene="yfcC"
                     /locus_tag="NK788_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCH6770834.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1929..3113
                     /gene="iadA"
                     /locus_tag="NK788_002534"
     CDS             1929..3113
                     /gene="iadA"
                     /locus_tag="NK788_002534"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCH6770835.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3224..3427
                     /locus_tag="NK788_002535"
     CDS             3224..3427
                     /locus_tag="NK788_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCH6770836.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1098 a          582 c          814 g          965 t
ORIGIN      
        1 aaattactga aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac
       61 cgaaacgcga cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag
      121 tcatctttgc atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata
      181 ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct
      241 tccgttatgc ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag
      301 atcatccagc agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg
      361 ctgacggaga aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg
      421 tgctttacga tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag
      481 ccaaaatatg gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag
      541 gactagtatc tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg
      601 ttggaggtgc ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt
      661 ttatcagtaa aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt
      721 cattcggtgg cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg
      781 ttccatttgt cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg
      841 catctcaagt cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag
      901 ggattgctgg aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg
      961 ttactgcatt agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag
     1021 aaagttcgtt gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg
     1081 atgaaaataa aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc
     1141 ttgtctggat cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc
     1201 aattctttgt gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa
     1261 tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag
     1321 caatcgtagt aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg
     1381 ttccttcagc tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag
     1441 ctgtcattgg tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat
     1501 caaattcagg acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag
     1561 gtgtatccag acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt
     1621 ttacacccgt atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa
     1681 aatgggcaag attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca
     1741 tcatgattgc cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa
     1801 tccaaacagt agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa
     1861 gatcctatcg tttggattat tttttaagat cctatcatca gttatactta aagaaagagg
     1921 aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa
     1981 tggatgtact gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct
     2041 acgaaggagt ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca
     2101 ttgactgtca tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc
     2161 cagaagtcac attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg
     2221 gaacagacgg agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag
     2281 cagaaggaat ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa
     2341 cagattctat catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg
     2401 cagtatccga tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag
     2461 atgctcgtgt aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg
     2521 gaggaaaaag aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca
     2581 cacaattttt gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat
     2641 ttgccaaacg tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga
     2701 agacagatgg tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt
     2761 ctttagataa tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa
     2821 acaatcattt cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag
     2881 aagctgtgca aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc
     2941 cagcacgtat attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc
     3001 tgtgtatctt ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa
     3061 tggtccaaga aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag
     3121 ctctaactat cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta
     3181 ccctgaaggt agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg
     3241 acgttctaaa gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc
     3301 aacagacgac aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa
     3361 agaaaaagct cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa
     3421 aaaataaaaa ccacttatta ctaattgaat caagcaaat
//
LOCUS       DAHUWM010000228         3456 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHUWM010000228 DAHUWM010000000
VERSION     DAHUWM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.233"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            200..982
                     /gene="rpsB"
                     /locus_tag="NK788_002536"
     CDS             200..982
                     /gene="rpsB"
                     /locus_tag="NK788_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCH6770837.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1081..1962
                     /locus_tag="NK788_002537"
     CDS             1081..1962
                     /locus_tag="NK788_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCH6770838.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2098..2820
                     /locus_tag="NK788_002538"
     CDS             2098..2820
                     /locus_tag="NK788_002538"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCH6770839.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2823..3380
                     /gene="frr"
                     /locus_tag="NK788_002539"
     CDS             2823..3380
                     /gene="frr"
                     /locus_tag="NK788_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCH6770840.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1265 a          601 c          768 g          822 t
ORIGIN      
        1 caggtaaact attgaatgtt gagcagaaaa gtatatttgc tcttgcgata cggatctttt
       61 gtctgtataa tagctgaggg tgctttatgc atcagcaaat ccacattctt attggattgt
      121 cggtaggtgc ttgaaaaagt ttgccggcaa ggtagaagat aagaagagga aaaataaaac
      181 caaatcggag gaaacaaaca tggcagtaat ttctatgaaa caattactag aagccggcgt
      241 acactttggt caccaaactc gtcgctggaa tccaaaaatg aagaaatata tcttcacaga
      301 aagaaacgga atctacatca ttgacttgca aaaaacagtt aaattagtag atgcagctta
      361 tgattacatg aagagcgttg ctgaagaagg cggcgtagca ttattcgtag gtacaaaaaa
      421 acaagcacaa gaagcaatca aagaagaagc aactcgtgca ggacaatact tcgttaacca
      481 tcgttggtta ggcggaacat tgacaaactg ggatactatc caaaaacgta tcagccgttt
      541 gaaacaaatc aatgcaatgg aagaagatgg aacttttgaa gttctaccta aaaaagaagt
      601 tgctggtttg aacaaagaac gtgaacgtct tgaaaaattc ttaggcggta tcgctgatat
      661 gccaagaatc ccagatgtaa tgtacatcgt tgacccacgt aaagaacgta ttgctgttca
      721 agaagcacac aaattaaata tcccaatcgt agctatggtt gatacaaact gcgatccaga
      781 tgaaatcgac gtagtaatcc cttcaaacga cgatgcaatt cgtgccgtta aattgattac
      841 ttcaaaaatg gctgatgctt tcatcgaagg aaaccaaggt gaagatcaag tagttgaaga
      901 agatttcaca gaagaaaaca cagcaacatc aatcgaagaa atcgtagatg ttgttgaagg
      961 cgacaattct tcagcagaat aattttgaat gatggagctg tttcaaaggc tgggcgacaa
     1021 gcctcactct cctttgaagc agcttttttt aaaaagaaaa acacacagga ggagttaaac
     1081 atggcagacg ttacagctaa aatggtaaaa gaactacgcg aaatgactgg cgtaggaatg
     1141 atggatgcga aaaaagcatt agttgaagta gaaggcgaca tggaaaaagc agttgatctt
     1201 ttacgtgaaa aaggaatggc aaaggctgcg aagaaaaatg accgcattgc agctgaagga
     1261 ttggcttcag ttgctgtgaa aggtaacact gcagcaatcg ttgaagtaaa ctcagaaaca
     1321 gactttgttt ctaaaaacga aatgttccaa gatttagtaa aagaaattgc agaacttgtt
     1381 gctgaaaaca aaccagcaga catggaagct gcaatgaaaa tcaaaacaga taaaggtaca
     1441 attgaatctg acttgatcga agcaactcaa gttatcggag aaaaaatcag cttccgtcgc
     1501 tttgaagttg tagaaaaaga cgacaatgct gcatttggtg gatacttgca tatgggcgga
     1561 cgtattgccg tattgactgt tctagatgga acaactgacg aaacagttgc aagagacgta
     1621 gctatgcacg tagcagctat caatcctcgt tatgtaaacg aatcacaaat tccagaagct
     1681 gaattagaac atgaaaaaac tgttcttaca gaacaagcat tgaacgaagg caaaccagcg
     1741 aatatcgttg aaaaaatggt agaaggacgc ttgaagaaat tcaaagcaga aatcgctttg
     1801 gtggaccaac cattcgttaa agatcctgat atgactgttg aaaaatacgt tgcatcaaaa
     1861 ggcgcaactg ttaaaacatt cgtacgtttc gaagttggcg aaggtatcga aaaacgcgaa
     1921 gataactttg tagaagaagt tatgagccaa gtgaaaaaat aattctgttg aataacagac
     1981 gggaacgcac ggtttgtgcg ctcccttttt ttaagcaaaa caagaaaaac cagtcttaga
     2041 gtgtcgaaat catctaagta tggtaaaatg gcaatagaaa atattggagg aatgaacatg
     2101 gtcacaccga aatatcagcg tgtagtctta aaattgagtg gagaagcttt agctggggac
     2161 gcaggatttg gaatcaaacc accagtaatc aaagaaatca tccaagaaat caaagaagtt
     2221 cacgagctgg gcgttgaaat ggcaatcgtt gtgggcggcg gaaatatctg gcgcggacaa
     2281 atcggagcac aaatgggcat ggaacgtgct caagctgatt acatgggtat gttagctaca
     2341 gttatgaatg ccttagcttt acaggatacg ttggaaaacg tgggtgtacc gacacgcgtg
     2401 caaacttcga ttgaaatgcg tcaaatcgca gagccatata ttcgccgtaa agcagaacgt
     2461 catttagaaa aaggacggat cgttatcttt gcaggcggta ccggcaaccc gtacttctcg
     2521 acagacacaa cagcagcatt acgcgcggca gaaatcggcg cagatgttat cctgatggct
     2581 aaaaacaatg tagacggtgt ttattctgct gatccgaaag tagatgcaaa tgcagtaaaa
     2641 tttgaagaac tgactcatct ggaagttatt gccaaaggat tacaagtcat ggattcaact
     2701 gcaagttcac taagcatgga taatgatatt ccactgcttg tcttcaattt aaatgaacac
     2761 ggaaatattc gccgtgcaat cttaggcgaa aatatcggaa caactgtaag ggggaaataa
     2821 aaatggcaga tgcaattatg acagaagcaa aagaaaaaat gaaaaaagca gcacaaaatc
     2881 ttcaacgtga acttgggcaa atccgcgcag gccgtgcgaa cgctagcctt ttggaccgta
     2941 ttactgtgaa ctactacggt gcgccaacac cattgaacca aatggcatca atccaaattc
     3001 cagaagcacg tgttttaatg atcacacctt ttgacaagag cattcttcaa gatgttgaaa
     3061 aagctattat ggcaagtgat atcggaatca gtccaaccaa tgatggaaat gtcattcgct
     3121 tagttatccc gcaactgaca gaagaacgca gaaaagaatt agctaaagat gttaaaaaag
     3181 aagcagaaaa tgcaaaaatc gcgattcgta acatccgtcg tgatgcgatc gatgaatata
     3241 aaaaacaaca aaaaaatggt gatattaccg aagatgattt gcgcggatta gaaaaagacg
     3301 tacaaacatt gacagataac agcatcaaag aagtagataa gatcgctgct gataaagaaa
     3361 aagaattatt ggaagtttaa taaaatcata aaaacagagg ggcggatcat tccgcttcct
     3421 ctgtttttat ttatatttct tttatttaaa aattaa
//
LOCUS       DAHUWM010000229         3435 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHUWM010000229 DAHUWM010000000
VERSION     DAHUWM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.234"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            93..1139
                     /locus_tag="NK788_002540"
     CDS             93..1139
                     /locus_tag="NK788_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCH6770841.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            1141..3294
                     /locus_tag="NK788_002541"
     CDS             1141..3294
                     /locus_tag="NK788_002541"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCH6770842.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         1066 a          673 c          665 g         1031 t
ORIGIN      
        1 ttatgtctac gctttcaaaa aatatctttt tatttacttt ttcatccatt atggtattat
       61 ttgaatatga tatatcatta agaggtgttt atatgactca accattgtac cagacaatat
      121 ttaatgattt gcaaacggct attttaactg gtgaattacc tgtagacagc caactaccaa
      181 ctgaaaaaga actttcacgt acctataaag taagcagaat cacttccaaa cgtgcattga
      241 ctgaattaga gcaattagga cttatttatc gcgttcgcgg gaaaggcagt ttcgttaaat
      301 ccgcttctcc ggaacagatt cttgcttctc ctacaaaaac acgacggatt ctgtttcttc
      361 ttccatatgt cgctgatttg tctgttggtg attttagcaa gggattgaac cctgtgatgc
      421 aagataaaca gattgatgtc atgatgacga cgcttgactt tctacaaaat aaaacagcaa
      481 aagagctaat caatgaattt gacggattga tttattatgc tcttgataca gaacaacatc
      541 ttgacttact ttttgagctt tcattgaaag aattccctgt cgtcatatta gataaaaaaa
      601 tctatgagtt accttttcct accgttcttt ccgataattt ccaaggcggt gcgcttgctg
      661 cctcttattt gatcgaacag ggacattcaa aaatcgctta tttgtttggt acgcagactc
      721 atccacaatc cgttcgacag cgttaccttg gttaccttga agcattggac aaagctggtt
      781 tgactttcca cacttcactg gatgacaaaa gcgcgacatt agatgaattg ctttcttata
      841 tcactaagta tgaagtaact gcttttgttt gtgaaaacga tgttgtcgct atccagacca
      901 tgaatctctt gagacagcat ggttatcata tccctgaaga tttttcaatt atcggatttg
      961 atgatatcca agcagcagct cttgttgacc cgccactgac aaccatcgca caagattttg
     1021 aacagttagg taagcttgcg ggagatgctt tggtcacttg gttagagaca aaagatcgac
     1081 catccgatag taaatttcca gtttcattca tcaaaagaca atcaacaaag gagaaataaa
     1141 atgaatatcg gtcaaataga tactagacat gcaacagcga atcagccaaa tttctctaat
     1201 gggaactgcc agccttatac tggcgttcct tttgggatga attattttgc accgcaaact
     1261 acggatcaaa acggcagctg gtggtttcat cctgatgacc gcgtttttca aggctaccgt
     1321 cttacccatc aaccaagtcc ttggatgggt gatttcagtc atatgctgtt tacgccgatc
     1381 aatggaaaac tacaggagaa cacccttttc catgctcaaa gttcttaccg cccagaagaa
     1441 agtatatttt gcccgactca tctgtcgatc tgtcaattac gttatggcat ctgttctaca
     1501 ttgataccta gcatgtatgg cggaatctta tctattgatt acagcaaaaa tgacagcggt
     1561 ttacttttat cctttccggg acgccatcag ctctttgtta tagatcctta cacagtggaa
     1621 ggaaaaatca cacattttgc tggttcagaa gatcctgatt tttcttttta ttttatattg
     1681 agatttgaat atcctttaac aattgatgag ttagctgttt ccaacaaaaa tagcgactcg
     1741 atcccgttgt attttgaaca gacaaaaaaa caaactatcc gatttgggac atcttttatc
     1801 agctcggaac aagcagctta taacctggaa ctagaacaaa accaaagcaa agagacttac
     1861 cttgctcaca gtcaagaaca atggcagtcc tattttgatc gtatacagct cacacaccat
     1921 gatcaacaac aattgcggac gttttatcat aatttatatc gggtgttttt atttccacaa
     1981 acattttatg aaattgatcc tagtgggcaa aaaatccact atgatacgac tagtcgatct
     2041 ataaaacctg gtgttctcta tacaaataat gggttttggg atacgtataa aacggtttat
     2101 cctctttatt ctttgattgc tgtagaaaaa tacgaagaaa tgctggaagg ttttttgaac
     2161 agctatagag aaagtggcta tttgccaaaa tggctgtctc ctgatgaacg tggacttatg
     2221 cctgggacat tgatcgatgc ggtcattgca gatgctgctg taaaagatat ccgacgagac
     2281 ttgatgcctg aatttctaac ggcaatggaa aaaggtgcta cggttcaaag caaacatcca
     2341 aattatggac gccaaggaac agaagattac ttgacatatg gctatgttcc taatcactac
     2401 cacgaatcag tcaatcacac attagactat gcttatagcg atttttgtat tagccaagtt
     2461 gccaaggtac tagaagaaga ggaaatgacg aagaaatatc agcagcaatc attgaactat
     2521 caaaatatct ttgactatga gtcaggattt atgcgttcaa aagataaaga tggcaagttc
     2581 agagaaccat tttcagctaa tcgttgggga caagattatg cagaaggaag tgcttggcaa
     2641 agcagctttg ctgtttatca cgatttttca ggactgatcg gaaaatatgg tggcagtgag
     2701 agatataaag aaaaactcat tgagctgtgc aatcagcccc caacgtttga cccaggagga
     2761 tatggttttg agatccacga aatgagtgag atggccgcga tagaatttgg acaattagct
     2821 atttcaaacc agccaagttt tcactatcct tacttgttca gttatatagg acaacctgaa
     2881 atggcacagc cactgcttaa acagttgatg actcagacct tcgacgattc tccaatgggt
     2941 taccctggag atgaggacaa tggaagtatg gccgcttggt atatcttcaa tagtctggga
     3001 ttttatcctg tcacgcctgg aaccggtgaa tatgttatcg gtatgccatt aattgacaag
     3061 gctgttttgc ttttgtcaaa tggaaaggaa ttgacgattg aagcaacgcc taatcggccg
     3121 cagcaacaat ttatccataa aatcaaacgg atggacagag aatatacgaa attatatttt
     3181 acccatcaag atttgctgaa aggcggcacg atctcttacc aattagggat catccctcac
     3241 cctaagaaat atcaagcaaa agatttgcct ttttccttat caaactataa ttaacaagag
     3301 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagatcctca
     3361 tatttttcgt ttggttggtc ttattaccgc agatgcaagc ttttgaacac cgtttattga
     3421 gaggttgtga aatca
//
LOCUS       DAHUWM010000230         3391 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHUWM010000230 DAHUWM010000000
VERSION     DAHUWM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.235"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(22..2493)
                     /locus_tag="NK788_002542"
     CDS             complement(22..2493)
                     /locus_tag="NK788_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCH6770843.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="NK788_002543"
     CDS             2766..>3391
                     /locus_tag="NK788_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6770844.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DAHUWM010000231         3376 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHUWM010000231 DAHUWM010000000
VERSION     DAHUWM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.236"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            375..1814
                     /locus_tag="NK788_002544"
     CDS             375..1814
                     /locus_tag="NK788_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6770845.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2109..2327
                     /locus_tag="NK788_002545"
                     /pseudo
     CDS             2109..2327
                     /locus_tag="NK788_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2576..2791
                     /locus_tag="NK788_002546"
     CDS             2576..2791
                     /locus_tag="NK788_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770846.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3008..3264)
                     /locus_tag="NK788_002547"
                     /pseudo
     CDS             complement(3008..3264)
                     /locus_tag="NK788_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          996 a          587 c          715 g         1078 t
ORIGIN      
        1 tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa acaaataggt
       61 gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaatttgc ttacgtctca
      121 cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga aattgctaga
      181 agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa gcatctccaa
      241 cttacatggt tacatgagga tatcaaatcg aaattactca agcttcctat ggctagagtc
      301 gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac gatcattaga
      361 aaggaaatcc tattatgtat agttatcaag aagatgcaaa agtccaaaaa aatcgctggt
      421 ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc agtattgtca
      481 atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa gcggaatggg
      541 tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt aaaattggcg
      601 atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc ttgggatcgc
      661 tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt cgcacgggtg gtacaagcca
      721 ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc tttcccatac
      781 atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt ttcattaggc tcgattgctg
      841 gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt ttttggatca
      901 atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca aaagatatca
      961 ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct acgattatca
     1021 gcttttttgg agcaatcttt ttggggcaag aaagtggctt tttccatcca gtcccccttg
     1081 tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa aagaaagcag
     1141 cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg agcttgatta
     1201 cagccttgtt gattttttct tccaacttct ttgtcaatgt agtgattcct ttttatctgc
     1261 aaaatgctcg cgggttgagt cctagctatg ctgggctgtt gatgatggtc tttcctttga
     1321 tgatggtcgt cggggcgcct ttaagcggct acctgaccga ccgggttggc ccggagattt
     1381 tggtattagt aggcttgggc ttattgtcgg taacccaggt catgtatatg tttttgtcag
     1441 tggactctcc tttgtggcta tacattgtcg ctactgccat catgggtgcc ggcaatgcct
     1501 tgttccagtc ccccaataat acgatggtta tgagctcggt cactatcgaa aacttgggac
     1561 tggcaggctc gatgaattcc tttgcccgaa atttggggat ggtcagcggg atttcgcttt
     1621 ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccagcaagta actaactata
     1681 tcgcaaatcg gccggatgtc tttttatatg gaatgaagat taccttttta ggcagctttg
     1741 ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa ggaggtattc
     1801 agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg aggcctgcgt atgggtgagt
     1861 gggatttcca tggatgatgg aagatgaagt gaacttctaa aagccgaaaa actcaagtat
     1921 tatcttcaaa atactagtgc tcaatgagat gcttgatttg atcacaagaa gactgcgaaa
     1981 gccgctggca accgcaagct aggtatccca ccgatggact acgttttaga ggctactggt
     2041 tatgaatacg gagccaacgc tctcatctgc ataagaatac atcacccaga ttatgtaaag
     2101 attttcgata cttctgtcaa aaaatcatta agatagataa atgatttccg tctagtcaaa
     2161 ttggttcaga atgtagacac acagatggta agaagtcact cacaattcga gaatggactt
     2221 gtcctatttg ccgtatacat cataatcgaa acgtcaacga tagtatcaat attttgacca
     2281 agggctatga atacaagcat tgccttagaa aacatcaaga accttaggaa caacggggat
     2341 agcttggtta ataagagata ccgctgttta ggcttgcaag tatactcttt tatcaagaat
     2401 tccgtggctt tagtcatgag aggcttaata ctatgaaaat tttctttagc atttttgatt
     2461 taggttcctt ttctaattat aataagatga ccactacagt aacctactga aatatataaa
     2521 cagccactgt agtaccattt tcaaagtagt ctatgagagg agaatcttca ttcttatgaa
     2581 cgctggaatt gcggatatga atttaataaa aaaaaccctt aatgatttta cttccaattc
     2641 aatatcaaaa ggtactggta tcaatttaag cacaataaaa aagctgaaaa gtggtgaaag
     2701 atcagtggaa aagctgaatc ttttagatgc tataaaaata acggaatttg ctatgaaaaa
     2761 tggaaaagct gagattgaaa tatggagata attatcgtct agtgaattac ttgccactta
     2821 gctgaattta acgatccttg gtgcttgaag ggggagattc ttgggaatag agcatacttg
     2881 caagcctatg tctttactaa caacgatatc tcttattaac caagctatcc ccgtagttct
     2941 aaggctttta cttttatcta agcaataatc gtattcaaag gaaatttttg acccttagaa
     3001 agtctcattt tatcagacgt gagcttaggc attgctgaat tttgttaatt cggttttagg
     3061 ttgtgctttg agcataatat gaaagtattc tttatcacga tttcattcat ctaaggtaat
     3121 gtgatgtgat tctgaaattc tttcaaaggc agtttttgca taagctgaca ttacatcatc
     3181 aatgacatgt ttacaatgtt tcactactaa aataaggggg taaccaagaa gaaaaactga
     3241 atgattattt gtatctaatt ccatggtata tcaaccattt cctatataga ctgattatga
     3301 catggattaa aataaaaagg cactatgtct taattgtcgg ttttcgagtt accgaatggc
     3361 attcatcacc caccta
//
LOCUS       DAHUWM010000232         3343 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHUWM010000232 DAHUWM010000000
VERSION     DAHUWM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.237"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(746..1702)
                     /locus_tag="NK788_002548"
     CDS             complement(746..1702)
                     /locus_tag="NK788_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770847.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1811..2056)
                     /locus_tag="NK788_002549"
                     /pseudo
     CDS             complement(1811..2056)
                     /locus_tag="NK788_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2201..2623)
                     /locus_tag="NK788_002550"
     CDS             complement(2201..2623)
                     /locus_tag="NK788_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770848.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2803..3174)
                     /locus_tag="NK788_002551"
     CDS             complement(2803..3174)
                     /locus_tag="NK788_002551"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCH6770849.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         1019 a          718 c          544 g         1062 t
ORIGIN      
        1 tcactttata ttactttctc ttgagtaaaa aaacatgtga ggtcgtaaac aaagattgaa
       61 ctatctacat tcatactcta taatctgttt atttgcagac tgataatagc aaaagaaaac
      121 ttataatcaa ataatatcaa catttgatca cataatttga gattgaatgc tgatttacct
      181 atgttttacg taatggtctt tagttatttt tgctcataaa atgcataaaa cacgcgtttt
      241 tatgtgtgct tgaaatttct tggctctata atttctaaga ctgataaaaa tattctatcg
      301 gttctttttg caaataaaaa aacatataga agctccatta gattgaatca ccacaacaca
      361 atcaaaggag cattccgtat gcttgatttt cattctataa aactactttt aggaatcaaa
      421 gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga agtaccattg tttcatcgat
      481 catgagaaaa gcattcacct ctgtctgcag atgttcttat atcgctctgt cagtcctatt
      541 aataaagtac tgttttgttt tcaaaaaaac tattacgagt actttttaag agaatgcctt
      601 gcaaatattc atcacacacc gttatataag tcaaatagtt tctttcagtc gtcaagaaaa
      661 gattctcctg tccttggcat tatcagttaa gtagcaactt aattctcgga cttgttctac
      721 tctagcaaaa tttattttca tcaacttacg tccaaatgtt tttttgaata attacttcat
      781 cgcgggtctg gaagacttgc ctcattcgat aagtcactag cttttggcgt ccttcaggat
      841 ttctagtata caccaactga catttgggca gcgacgattg atttaattcg tttagaaaaa
      901 ggttagcgat ttgcttattt cggccaaagc gttctggtac cgtcagaact atctttttcc
      961 cttcgattaa ataccgcgga ttctccagcg gtgctaaaag ctccctcata gcttcagtaa
     1021 aaagctgtcc ttctcgatag ccagcattct caagatagca tctaaattcc tgctgatcag
     1081 ccgctacgat tacttttcta tcactaactt caccaaggcg ctgcaacgtc gtttgaacgc
     1141 atatggccaa gctttttttg cgggcacatt ccttttccca tagcttttgt cgcctacgaa
     1201 tttggaaata ttccagcaag ttacctttaa atatagctga ccagaagctt agaggctcta
     1261 tattttttac tactatcggc aatgaagttt cttgtttttg ctttacctga tcgactttcc
     1321 taacgacgat tggcatagat aggtgccagc catcagctaa agcagtactc cattgttctt
     1381 tcaaccgact tcgttcacaa gaaaaataga acatcgactg agtataagtt tccatcgtgg
     1441 tggtcgtata ttccagtggt aaagcaaagc gatcgccacc actctcaatt tttggtgaag
     1501 attcaaaggt cagacctgaa atattgccca aacgccgtag cattcttttc aaatcaggct
     1561 gttcagacag aggaactccc ggtaatccaa tcgctaaatg ccaaatgttc gttactttat
     1621 tttgtgcacc taggcgtaag ccccgccctc gtagttgttg tgtttgcaca aaagtggaag
     1681 cttgattagc caagatcaac agattaattg caggacaatc ccagccttct cctagcaaac
     1741 taacggttcc taccaaaata ttcaacttct tttcggttaa taggcgagta actgcttgca
     1801 tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa ataacccgac atataaggat
     1861 gttcattcat acccgtcgct agttctgcca gctcaacagt tactagcatt ttcggtaaca
     1921 gtaagaactc tccacagatg gcaccgattt gctgagtgtt acctaataga cgaacaactt
     1981 catcaaacaa aggaaccacg ccataagcta aggtaccatc actgcctgct aatacctcct
     2041 tgcaaatacg atccatcaaa atgaccgctg tcagttcttg cttcagctga acttcttctt
     2101 ttaacaaaat ttgacaaact gcttgctttt tctcaggaat cgcctgacta ccaacagtgt
     2161 ctggatactc tggaaacaaa tgcagcgact catcaaacaa tcatccagct tttttcaatt
     2221 gataaaaaat aggcaattcc ggttgatctt caaacatctg ctgataaata gtgatcagct
     2281 cttttttacc taaagatgga actgaagctt ttttaccttg ctgcttagaa ctgcctaata
     2341 gctgccaatg ttcgggtatc aattcatagt tatgatgatt caagtaaatt aatgcactag
     2401 tgaacagctc aaaattttca taaatttcct tcgaatgctc aagcggatgc tgaacaaatt
     2461 ctaaagaaag caaataatcc accgtctctt ggctttccat taacgcttcc accagctcat
     2521 tttgtttctc taaaaaggta aaataatgct gagtagtttc ctgattagcc ggaatcaaat
     2581 atagaaaatc ctgatgagga catagaacct cctcctttac caactcggga atgctaattt
     2641 cttcgtcaat caggccattt taactcaata tattgcttcc actccgtctg agagctatca
     2701 atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg gttgtttttt cttcaagtca
     2761 atcaacaatt caccccagct tttttttaaa tgatgagctt catcaaaaaa ctaacagctc
     2821 cagctgatca ccataagttg gctcctcctt cagtcggttg taaatggtct gatacgtttc
     2881 cactgtaata tgactaggat gttcaatcgc cccagaaaga tagcaactat ctgctggatt
     2941 tactaagaaa tattcctcga ttgttgttaa ccactgtttc ttcaatatta gggaaggaac
     3001 caaaattaag gttttacaat ccaaacgtcg gatcatctca attcctaaaa aggtcttgcc
     3061 agcccctggt ggagccacta gatggactga attgttattt aaataagtag caaagttagt
     3121 caatatacgc ttctgatagc tacgccactg aattttttca tcagatagcc acatcccagg
     3181 tccccctctc acgtattttc tctatcataa tctaaatgaa tacagcaaaa aaaagatttc
     3241 ttcaagaata tgtgttttta tagtaatatt ttacttactc taaactcaaa tgaaaaggta
     3301 aaaaaactgg tagtacaatc ttttaaattc tcattgtttt gta
//
LOCUS       DAHUWM010000233         3341 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHUWM010000233 DAHUWM010000000
VERSION     DAHUWM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.238"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(303..1277)
                     /gene="sgrA"
                     /locus_tag="NK788_002552"
     CDS             complement(303..1277)
                     /gene="sgrA"
                     /locus_tag="NK788_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCH6770850.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1473..2312)
                     /locus_tag="NK788_002553"
     CDS             complement(1473..2312)
                     /locus_tag="NK788_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCH6770851.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2497..>3341
                     /locus_tag="NK788_002554"
     CDS             2497..>3341
                     /locus_tag="NK788_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCH6770852.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKD"
BASE COUNT         1115 a          692 c          500 g         1034 t
ORIGIN      
        1 aacaaagtac atactccttt ttatctatta tttagactta ttccgacagc tacatgaata
       61 acgtacaaat agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt
      121 caattattct tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg
      181 ttagtaaaca aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg
      241 aatgaaatat tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt
      301 ctttatttca ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt
      361 aacacaccca tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg
      421 ctttttctat tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa
      481 tcaggctgtt ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt
      541 ggtatgatag gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc
      601 gtgcttcctg tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc
      661 tccgtactgc tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta
      721 ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc tgtactgctt
      781 tcaccgctcg tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt
      841 ccaacaactg tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa
      901 aaaacaatca aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa
      961 atcattattg tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca
     1021 ataacttttt ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca
     1081 ctagcattca cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca
     1141 taaacaatcc gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca
     1201 gaagtactga ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg
     1261 gtcgctgttt tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa
     1321 ttatataaaa tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg
     1381 tcgtgtattc gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc
     1441 aagaccgata tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc
     1501 atccgctttg ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat
     1561 gtagttgata tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat
     1621 tggatcagag tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata
     1681 gctccttgtg tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta
     1741 ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat
     1801 gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct
     1861 attaacggac caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct
     1921 gatgccccca ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt
     1981 cctgaatcta accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact
     2041 ccaattccaa caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca
     2101 ccataatctt ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa
     2161 acagctaaat gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac
     2221 atatccgcaa gtgcattatt gacaagaatt cctaaaacaa aaccaactaa gactattaaa
     2281 acagatccaa tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta
     2341 acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa
     2401 tagattttta cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc
     2461 taattcttct gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa
     2521 ctactaatta tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc
     2581 aatgaaacga ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac
     2641 ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt
     2701 gatgatgtat acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa
     2761 atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag
     2821 acatataaga aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta
     2881 aaattaacag ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca
     2941 cagatcattc tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa
     3001 ggggaaaatt tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact
     3061 gatggaaaaa tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca
     3121 gcatttaaac taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact
     3181 tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa
     3241 gacaaataca aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa
     3301 ttcatggata aaacaatccg taaagtgatg aaaaaagaca a
//
LOCUS       DAHUWM010000234         3341 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHUWM010000234 DAHUWM010000000
VERSION     DAHUWM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.239"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(111..596)
                     /locus_tag="NK788_002555"
     CDS             complement(111..596)
                     /locus_tag="NK788_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCH6770853.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    686..1031
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1076..1717
                     /locus_tag="NK788_002556"
     CDS             1076..1717
                     /locus_tag="NK788_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCH6770854.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1736..2371
                     /locus_tag="NK788_002557"
     CDS             1736..2371
                     /locus_tag="NK788_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCH6770855.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2389..3204
                     /locus_tag="NK788_002558"
     CDS             2389..3204
                     /locus_tag="NK788_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCH6770856.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1085 a          555 c          721 g          980 t
ORIGIN      
        1 gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct
       61 aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt
      121 tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata
      181 atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca
      241 gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact
      301 gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc
      361 ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg
      421 acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca
      481 ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa
      541 gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg
      601 catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc
      661 atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg
      721 tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg
      781 agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt
      841 tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc
      901 acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca
      961 acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat
     1021 acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga
     1081 gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt
     1141 tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt
     1201 gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc
     1261 gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga
     1321 tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat
     1381 ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca
     1441 attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt
     1501 accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt
     1561 aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact
     1621 gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcataat
     1681 gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat
     1741 tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct
     1801 aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac
     1861 gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg
     1921 tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca
     1981 agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat
     2041 ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt
     2101 agggattagt gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg
     2161 ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac
     2221 aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga
     2281 agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga
     2341 tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc
     2401 agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa
     2461 acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac
     2521 gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt
     2581 gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac
     2641 tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat
     2701 tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat
     2761 caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag
     2821 agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta
     2881 ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg
     2941 atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat
     3001 tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt
     3061 gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa
     3121 gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc
     3181 gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc
     3241 cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca
     3301 ggctcacaac ctcttggtaa acggtgttca aaagccagtt t
//
LOCUS       DAHUWM010000235         3276 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHUWM010000235 DAHUWM010000000
VERSION     DAHUWM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.240"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..2232)
                     /locus_tag="NK788_002559"
     CDS             complement(<1..2232)
                     /locus_tag="NK788_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770857.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="NK788_002560"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="NK788_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          897 a          732 c          534 g         1113 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttc
//
LOCUS       DAHUWM010000236         3258 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHUWM010000236 DAHUWM010000000
VERSION     DAHUWM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.241"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(117..347)
                     /locus_tag="NK788_002561"
     CDS             complement(117..347)
                     /locus_tag="NK788_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770858.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(441..779)
                     /locus_tag="NK788_002562"
     CDS             complement(441..779)
                     /locus_tag="NK788_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770859.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1707..1823)
                     /locus_tag="NK788_002563"
     CDS             complement(1707..1823)
                     /locus_tag="NK788_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCH6770860.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2299..3177)
                     /locus_tag="NK788_002564"
     CDS             complement(2299..3177)
                     /locus_tag="NK788_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCH6770861.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1034 a          597 c          472 g         1155 t
ORIGIN      
        1 aaataaaata ctctggatac tctttatact gcattaaagt tagtaatatt aacagcaaag
       61 atatcgaaaa aacaataaca taatatgtgt caaaattttt cataataaga actcccttac
      121 tgttgcatac taacataatc caaaaactct tctacttcag ctaaagacat tccactttct
      181 ttagtccaaa aaacagtttt ttctatcgat ccttctctgt aaactgtaaa tgtcggagtc
      241 gagttaattg agtagcgact cttgaattct ttccactcat tttcatcttc tctaactttt
      301 gctacattca cgtaataaat attttctttt cccttttcta ccagcatatc gaatagcttc
      361 ctgcttttga tgatcgattt cttgtgctac atctagtaaa ttttcatcaa tttctatatc
      421 ataaagtatt tcactagtgt ctacatccac aatatatact ttttatcagt agcaattttt
      481 gagtaatccg ttaaaatgat ctcagtctcg tcactttcat cagtataaat atacgttaac
      541 aatgaactca ccttttcaat cggtgttagc tctaagccta ctgtattttt tactattgag
      601 tcattattta ccaccataac ttcgtactgt atataccatc ctatttggtt tccatcaata
      661 tcagtaattg gctcactagc taatggagtc tccggaacaa tattttcctg atatttctta
      721 aactcttctg tagcacgaaa aaggttatca tgttcctcac ttgaaactgg ttgagtcata
      781 gaaaatgtta cttggctaaa cagagggaaa ataaacacta caatcaacaa agtataaaaa
      841 aaagcccttc tttttcattt tttatcttcc aatcacaaat tgaaaaactt attcctgcaa
      901 ctttgagtct agtatcacaa tcgactgact acaactattc atttacattc tactttaagt
      961 tcctccttaa ccattaattt tattaaagat acttgtaata gtgattctca caaaatttca
     1021 tgaaaaatac tttattactg tatctaaaat tccaatacac aacttatcga ctattatctg
     1081 aaaaccaaaa cttattattc cttgatgtta ttctagcaat agtaactgct tttttatttt
     1141 tttgcatatt ttttcggcgt tactttttaa agttaacttt ttcgacaaat agaaagagat
     1201 acgaatcact gatttttttg aagaaaagaa atggagtgag ctgaacgtta gagatattgg
     1261 gagctttcgt aactgcttat taaacgataa tggttatttg caaccgtatt gctcgcttct
     1321 acatgaaata tttcatgaaa tatttcgcaa aacctttgat tatgtagtta cgtttccatt
     1381 cttgactaaa aatgtttcaa aaacgaacaa aggtgttaaa aggtaaagtg atcaatacta
     1441 ctggtcaaaa taagaggttg aaagggaatt gaaaaataac tacagataat acttttataa
     1501 acgtacgtgc tttgtggttc tgtagttaac tatataactg gaattaggga atggtatcaa
     1561 tttgaaaaga aagattctac caatttatca tatgttaaaa atggaaaatc aacagcagtt
     1621 ctttcggaaa ccttacacaa aaaccgatta ttgaaaatag ccgaaaaacg tttttcggct
     1681 atttttttaa ttaagtttcc ttcttcttac cttttcccac tattccgttt cacatctttg
     1741 atgatcttca gcgcacgttt tcgtgttttg atatttgggc ttttcaagcg gcggtatgca
     1801 cttgagatat ctgttttttc catatggacg acccactttc ttctttgatt gtagtcatgc
     1861 taaaaaacac aaatcgaaac aattacgatt tgtgttttaa atggtatcaa aagttaaaat
     1921 gaaagtcaac ttgttaaaac agtcgatcag actagctgat tgactcctat ctctggaaat
     1981 aaattttcat tattacgttt tagctaccta aaaaaggccc tcttgtccgt taactttaat
     2041 atctattgtt atagctagtt tacctattga acgtactgtg tgttcgatag aacactaaaa
     2101 aagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct
     2161 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgct tccgccgttt
     2221 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tcttttaaac
     2281 tatttcgaag tactaaactt atgaagtaag aatacctaag ccagagccaa tgtgctcgcc
     2341 tgcaagcaat tgctcttttg cttgtcgctg taaactcgta ctttctggat aatcatgtat
     2401 ttgggcatct actcgtccaa agatccaagg gactccacta tcgtcctgtt catcaactga
     2461 taataatcga taatcggctg caaaaagaca gatttttgac tcttttttca accatttagt
     2521 aagattgggt gatagtaacc aagtatctgt gtacatcact tggtaatggt aatcaggaaa
     2581 ataaagttca aaaaaacgaa tggcactttc gtaactatct cttatcttat tctgagataa
     2641 atctgcatcg gaaggaatat gaagagaaag gcactgcttt ccttctaatc ttagatccgt
     2701 gtaccctttc ggaaaatatg tcatttcgta ctccaattca ccaatccgaa agatcaagcc
     2761 actagtgtaa tgccaaatcc aaaaaccacg gtcaaagcga tagcttcctg ttacttggaa
     2821 atgttcatga ataaatctag taaaagcatt cattgttgcc aaatagatag ttcttggaat
     2881 cttcaatggt tgataaattt ttttccaact ttgagaagct gcatatagat aaatagctaa
     2941 tgctgcaaaa ggttcctctt catagacttt gtctattttc tttgcagtcg cctttgcttt
     3001 attaggatcc attagttcat gactatattg ctcaaacacc tcatcaagga tgttttttgc
     3061 ttgtacaatc atttgctcta taacgacttt gggaagagtt aaagtttctg ccagaatttg
     3121 gatttcattg cttattttct tcatcaaatt tccccttttc atatctttga acctcattgt
     3181 ttaaatgact gattatccgg acaccatatg gtataaacat acttttattg tacactaact
     3241 ataaaactta aactttct
//
LOCUS       DAHUWM010000237         3258 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHUWM010000237 DAHUWM010000000
VERSION     DAHUWM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.242"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..183)
                     /locus_tag="NK788_002565"
     CDS             complement(<1..183)
                     /locus_tag="NK788_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCH6770862.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLIT"
     gene            341..604
                     /locus_tag="NK788_002566"
     CDS             341..604
                     /locus_tag="NK788_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770863.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            628..816
                     /locus_tag="NK788_002567"
     CDS             628..816
                     /locus_tag="NK788_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770864.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(899..1084)
                     /locus_tag="NK788_002568"
     CDS             complement(899..1084)
                     /locus_tag="NK788_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770865.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1299..1670
                     /locus_tag="NK788_002569"
     CDS             1299..1670
                     /locus_tag="NK788_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCH6770866.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1820..1892)
                     /locus_tag="NK788_002570"
     tRNA            complement(1820..1892)
                     /locus_tag="NK788_002570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1857..1859),aa:Gln,seq:ctg)
     gene            complement(1938..2366)
                     /locus_tag="NK788_002571"
     CDS             complement(1938..2366)
                     /locus_tag="NK788_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770867.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2426..3184)
                     /locus_tag="NK788_002572"
     CDS             complement(2426..3184)
                     /locus_tag="NK788_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCH6770868.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1079 a          520 c          612 g         1047 t
ORIGIN      
        1 tgttattaat acagacgttg tttcccaatg cctaaaagaa aaaatttgat aagtaaaata
       61 tacagcaggt aaagaggcca gtaaaactga aaacttcttt tttgtccatt ttatgtaata
      121 gaaaagaatg cttatcataa taaaaaaagg aatataaaaa acaaattgac cgatcgcgat
      181 catactattg cctccgataa ataaagtcca tgatacaaaa taacactttg tgcgaaatgg
      241 cgcaagcagt tttgtggatt tatccggtaa aatctgtatc gattttgaaa ttagaggaga
      301 aacttgctat aatcaaagtg aaagaaggag tgggattttc atggatcatg tagtgttaga
      361 aatcgatttg catttgatca ataatattcg ggtgatttac tatgttgtaa gtaacagtgt
      421 cgaacaacgt gtactgacga ataaaataaa tggtatttta gcaaagaaag acgttcaccg
      481 attcaataat ggtgaaggaa gttattatag tatcccagta gaaaaaatta tttatacgac
      541 agtcaaagtc agagaagatc tagataccaa aaaagcatat gaacctattt ttacaactta
      601 ttgattgaca gcagatggag gaaaaaaatg aatccatatg aacaacttat tcaagatttg
      661 atcgatggaa aaatcgaaaa gattgatgta aaaagagaag aattaatggc ttttcgagaa
      721 gcgtggctga agttggagaa tcgtaaatat gtggtgggaa aagcctcact gaacggcaat
      781 gtgacttatc attacgatcc gacacgttta ttttagtatg aagtattcag aaaagaacca
      841 aaggaggacg atgcagcagc attgtcctcc tttgatttta caaaagtgaa agttttactt
      901 atttgaatag atttttaaat ttgtttttga cactgtcgat atttacatct tttaaaagat
      961 tttgggcttt ctctttataa ggtcctaatt gttctttatg ttcttcgata aatcgttttg
     1021 cgccatcgaa attgccgttc ttaatcattt ccttgacttt ttgaaataat tcttcttgat
     1081 tcattttgaa cacatccttt gctatcaaca tgatttttta catggacaaa acgagaaaaa
     1141 ctgatttatc cacacgtcaa taatatcaaa taacgatcat ttttactaaa aaaacactcg
     1201 attctcgctt ctaaagtagg agaaaaatct ccaacttggg acggatggaa tttctttaac
     1261 tgacccatat actgaatata taacaaggag gtagaaagat gaaagtgatt aaatatttga
     1321 ttggactggc ggcgattagt ggtattttgc tatttggact gaaatattat actgaaggaa
     1381 gttatggaga aatccctggt atacttgatc agctaaatcc attagttgaa aaaggagagg
     1441 tctatgtaaa gacacaaaaa ccagaggaag taaatgaata tggtacagca aggtatgtgc
     1501 agaaagctgc tgatgccaat gggaaagaac gaacggttga atttaatggt ctttctgtgt
     1561 taaaagaaaa tcattatttg aaaattagca ataaaggagc acacatagaa acgtatgagg
     1621 aagttcctag agaaaaagtt ccggagaaag cattagcaat tattgggtaa atcagttttt
     1681 gtattaagaa tacgcccgat tttattattc ataatttaat tatgtaaact aaatacaagt
     1741 agtaaaaata atacaataat ccccctgcaa aaagattgtt cttttacagg gggttcattt
     1801 tttcagcaag cacatcaaat tgggatggca gggttcgaac ctacaattgc ttgattcaga
     1861 gtcaagtgcc ttaccagttt ggctacatcc catttattca acaaaataat catagcactt
     1921 tcctaaaaaa acgcaagtta tttttcttga tcggatagtt gttgctttac tcgtaaaagg
     1981 cggcgtacta gtttcttttc taatccagca ggaagggcaa tcagttctgc tactgacaag
     2041 acggtgtcca ataactgata ttctttgaca ggttcattca taatatgata gatgtttgct
     2101 aaacgttcaa tatattcaaa gcgagtatac tctgttcgag caattagaaa tttcaataaa
     2161 tcggtggctt tctcgtactc ttgatctgcg taataaacaa gagattgacg atacaatttg
     2221 atttccagtt cattgagggt agcaggtctg cgacgctcgt tccactcatt aatagagact
     2281 ttaccttgaa catttccctt gcagtcacgg acaaaaataa tcggaatttc atttggatcc
     2341 atttttttat gtgtgaattt ttccattatc tacgctccct tcaaaggttt ggtatcttga
     2401 gtataaggga tttttgtttg aattactagc ttttctccaa agttagcatc tgtccaccca
     2461 ttgcagtgaa aatcgccgaa aaatcgttga tcggtactgc tcgatttttt acaccatagg
     2521 gatcgttgac ataaatattt tctttgtcat atccagtgac tacacaagca tgaacaagag
     2581 gagtgactct tacttctcca ttatttgtgg tccaagtcat aaaatcagtt tcatctggga
     2641 cttcaaaatt gatagtcaca gaagtccaga caggatttcc tttagatagt gcttttttga
     2701 tatcatcgag ttgagcatcg gtttttggaa aagtgtgata atccgtttgg acgatttgct
     2761 gagcaaggga ggcaactggg ggaacataga cgaccatagc ccagtctccg ccactgatat
     2821 caccaacaaa tccttcatta ggatcgccat gagtcccatc tgcattgaaa acagggacgt
     2881 aatccaattg ctcagctaac tggtttttag ttgtttcata accataaaaa gataaaagca
     2941 tagaaagcga agtgacttca cagccatttt ctaaagaagg agaatcaaac tggctctcta
     3001 atggaacatc tagtaaaact tctgctttct cttttgtctt tttcgttttt tgtgttgaac
     3061 ttttttgtgc agtgttcatt gtatgttctg gagaatggat atgtaggtaa taacttgtac
     3121 cgcttaataa aagagcagct gtgcccccta gaaacaatag ttttttgaac gtttttttct
     3181 tcatttgaaa tctccctttt cagctgttta tctttcctat tataaatgta tttttattgg
     3241 aaatggaagt attatatt
//
LOCUS       DAHUWM010000238         3256 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHUWM010000238 DAHUWM010000000
VERSION     DAHUWM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.243"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..502
                     /locus_tag="NK788_002573"
     CDS             <1..502
                     /locus_tag="NK788_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6770869.1"
                     /translation="SDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLELNGKKVSLSN
                     ASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQILIVTH
                     NANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTNRAKRYG
                     FKQIVE"
     gene            552..941
                     /locus_tag="NK788_002574"
     CDS             552..941
                     /locus_tag="NK788_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6770870.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            928..>3256
                     /locus_tag="NK788_002575"
     CDS             928..>3256
                     /locus_tag="NK788_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6770871.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1053 a          546 c          699 g          958 t
ORIGIN      
        1 ttcagataaa aaattaatca attctataag aaaattgaat gatgcacaag ttcttgaatt
       61 acaaaccctt aagccaaatg atttagtagt tatccgactt gaactaaatg gaaaaaaagt
      121 tagtttatcg aatgctagtg ctggtcaaaa gacatctgca attctttcta tgattttggc
      181 gtatggaaat tcaccactca tattggatca gcctgaagat gatttggata gtcaattaat
      241 taataactta atcgtaagaa gtatcattac aaaaaaggaa aacagacaaa ttttaattgt
      301 aactcataat gcaaatatcc cagttaatgg tgattcagaa tggttgatta gcatgggaga
      361 ttcaaaagaa atatcggctg atcagtgcgg gtcggtagat gaacccaata tcaagccaag
      421 aatatgtact gtgatggaag gtggcgagga tgcttttaca aatagagcga aacgctatgg
      481 ttttaaacaa attgtagaat aacactagag ggtgagacat attattatgt ccgccctttt
      541 taggaggaaa aatgaaaaaa ataaaaagct ataccagtat ctgggcagtt gaaaaagtca
      601 tttacgcaat caatgatttt cagttgcctt ttcctgtgac ttttaaccaa atggcttggt
      661 tcgttttatc tttactgttt gtcatcgtat ttgcccatgt gcctccacta tctatgatag
      721 aggaggtctt tttaaaatac ttaggcatac cggtggctgt cacttggttt atgtcgcaaa
      781 agacgtttga tggcaagaaa ccactgggat ttttacgatc ctttatcagc tatcacttgc
      841 gatttaaggt gacttttgca ggaaagaaag tcaaagaaca gaagaaacga tgggatgaac
      901 ccagtactct tgtaaggagt gtgaactatg tatccgatta agtatattga aaataatctc
      961 gtctttaacc aagaaggcga gtgttttgcc tactatgaat tagtgcctta caattactcc
     1021 tttttgtcac ccgaacagaa atttcaagtg catgataact ttcgccaact aattgcgcaa
     1081 aatcgtgaag gcaagatcca tgcgttacaa attgccacag aaagtagtat tcgcgccaca
     1141 caagaacgat caaaaaaaga aattaccggt agactcaaag aagtagcgaa acaacgaatt
     1201 gatatacaga cagatgcatt agtatccatg attggcgata gtcagattga ttatcgcttt
     1261 tttattggct ttaaactaat tgcgacagat gaagaagtca atctaaagag tctcaagaaa
     1321 tctttctttt ctgggcttca agaatttgtc tatggagtga atcatcattt gatgggcgat
     1381 tttgtttcct tatccaatga agaaattcgc tgctattcta agcttgaaaa attaatggaa
     1441 agcaaattgg ctcgaagatt taaagtcagg cgagtcacac cgagtgacct gacgtatttg
     1501 attgaacata tttatggcga aaaagggata ccgtttgaag aatacgaatt tcagctacca
     1561 aagaaaaagc taaaatcaga aacactggtg aaacgttatg acttattacg tccaaatcgt
     1621 tgtttaattg aagaaaaacc aaggtgctta cggatggaac atgagaatca tgagtcgtac
     1681 gtagcgtatt taacaattaa tacaattgtg ggggagatgg aatttccttc ctcagaactt
     1741 ttctattacc agcaacaaca atttacattc ccaatcgaca caagtatgaa tgtagagatt
     1801 gtgacgaata aaaaggcttt agctaccgtt cgtaataaga aaaaagaact gaaagattta
     1861 gacaatcatg cttaccaatc tgacaatgaa acgaattcca atgtcttaga tgctttggat
     1921 tccgtggatg aattggaaac caccttggat cagtcaaaag aatctatgta caaactgagt
     1981 tatgtggttc gagtgagtgc cgaatcagtg gatgaactaa agcgacgctg tgatgaagtg
     2041 ctcgattttt atgatgatac caatgtaaaa ctagtccggc cctttggcga tatgatgggg
     2101 ctacatgaag aattcttacc ctcaagtaaa cgatatatga acgactatat tcagtatgtt
     2161 acgtctgatt ttttggcggg gcttggtttt ggggctactc agatgctagg agaattagaa
     2221 gggatttact ttggctacaa tgtggataca ggtcgtaatg tttacctgaa accggcattg
     2281 gcttcacaag gagtcaaggg ttcagtaacc aatgcgctgg ccgccgcttt cctgggttct
     2341 cttggtggag gtaaatcctt tagtaacaat ctcttagtct actatgctgt tttgttcggt
     2401 ggtcaagctg tgattgtcga tccaaaaggg gagcgaggag ggtggaaaga aaccttgcca
     2461 gaaattgcgg atgaaatcag tatcctcaac ttgacgagta agtcagaaaa tcaaggtctg
     2521 cttgatcctt atgtgattat gaagaaaaag aaagactcgg aaagtttagc aattgatatt
     2581 ctgacttttt taacaggaat ttcaagtcga gacggggaga agtttcctgt gttacgtaaa
     2641 gccattcgaa cggtgactca aagtgatcaa cgggggttat tacaagttat taacgaattg
     2701 cgaaaagacg ggtcaccaat cgcggaaaat atcgccgatc atatcgagtc ctttaccgat
     2761 tatgattttg ctcatttgct tttctcagac ggctcgatta cccaatcgat tagtttagat
     2821 aaacagctaa atatcattca agtggcggat ttggttttac cggatgcaga aacgactttt
     2881 gaagaatata ccaccatgga attgcttagt gtttcgatgc taatcgtcat ttccactttt
     2941 gccttggact ttatccatag taaccgagaa atcttcaaaa ttgtcgattt ggatgaggct
     3001 tggagttttc ttcaagtggc tcaaggaaaa acgttatcga ataagcttgt tcgtgctggt
     3061 cgggccatga atgctggggt ctatttcgtc actcaaaata gcgacgattt attagatgaa
     3121 cgtttaaaaa ataatatcgg gttgaagttt gcttttcgca gtaccgatat tcatgaaatc
     3181 aagaaaacct tggaattctt tggtttggat caggaggatc aaagcaatca aaaacgcctg
     3241 cgagatttgg aaaacg
//
LOCUS       DAHUWM010000239         3187 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHUWM010000239 DAHUWM010000000
VERSION     DAHUWM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.244"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            70..360
                     /locus_tag="NK788_002576"
     CDS             70..360
                     /locus_tag="NK788_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770872.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            373..1398
                     /gene="sppA"
                     /locus_tag="NK788_002577"
     CDS             373..1398
                     /gene="sppA"
                     /locus_tag="NK788_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCH6770873.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            1401..2060
                     /locus_tag="NK788_002578"
     CDS             1401..2060
                     /locus_tag="NK788_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCH6770874.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            2217..3149
                     /locus_tag="NK788_002579"
     CDS             2217..3149
                     /locus_tag="NK788_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770875.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT         1075 a          515 c          720 g          877 t
ORIGIN      
        1 ggtttttatc tatcatcttt ttcaggagtt cggtacaata gaaagtaatc ggatagagga
       61 ggagtaaaaa tggattcaaa aattgagatc atgacactgg gaatgttaaa aaaacaattg
      121 tccaaattcg aagcatcagc tggtgtatca gatgatacga aaatattttt agatactggc
      181 tgggatagca tccaagaaat cgcacctgat gccttggaag ttgctcaagc tcgtgagttt
      241 acggtagaag atgaattaac aaaagagtcg ttttccgggt atgcgcggga agaaaaagca
      301 gagcggttcg acacttctga gcagtcagaa actgtgatcg tcataaaaaa cttgtattag
      361 aaaggagaaa gaatgaataa aagacgttgg gcagcagttc tgattgctgc aggattattt
      421 gtggtttcgt tagtttcagc tagtttgact aagcctcagg aaaacgatca aattgaggga
      481 ttgaacagtt ggttatatgg ggatgaagaa ctagctccaa atgtgttgga agagggagac
      541 agtacgcaaa aaatcgtaaa attatcagta gatggaacga ttgctgcggg agggtcttca
      601 ggtttatttg cttctgaagg ttacaatcat aaaaatttta tggaacaact gaaggcgatt
      661 gaagaagatc caacagtcaa aggtatcttt ttagaagtaa attctcctgg tggcggtgtc
      721 tacgaaagtg cggaaatcgc taagaaattg gatacgattc gtaaagaaca tgatatccca
      781 atgtatgtca gcatgaaaaa catggcagct agcggtggct actatatttc tgctcaagca
      841 gataaaatct ttgcaacaga agaaacagtg accggctcaa ttggtgtgat catgtcagga
      901 ttgaattatt caggacttct agaaaaactg ggtgtggaag atacaacagt taaaagcgga
      961 gcgttgaaag atatgggttc aggaacgcga ccagagacaa aagaagaaga agcggtgctt
     1021 caagcatata tcgacagcgc atatagtcgt tttgtagcaa tcgtcagcga gggacggaat
     1081 aaatctgaag aagaagtcaa aaaaattgct gatggaagga tttatgatgg tgtccaagca
     1141 aaagaagtag gtttagtcga tgaattggga tttcctgaag atgctttaaa agctatacga
     1201 aaggaacaac agcttgaaga tgcggaacta gtagaatatt cttccacgcc atctgggttt
     1261 gctagtacat ggttaggaag taaactagca gaattccagg gattgaagcc ttctgagact
     1321 agtcagatct tatccgttct tgagaatcta gggacagcgg aatcaccaaa agcaatgtat
     1381 tattacggag gtgagtaagc atgtcagagc tttcaaatga aaataaggaa caagaaaaag
     1441 ataagcaaca gcagcctgct gtaggacctg actccaactt ttcccaaaag gtactgagct
     1501 cttttgaaag agagcctttg acgcccgaag aaatcaaaaa gaaacaacgt aaatggaaaa
     1561 agtatacgga cacaagaaat gaaccgccaa aagcaaatga ttttcctaag tatttctatg
     1621 caggtttttt tatccgaatg tttgccttct tggtcgatct tttgtgtatc ggagctatta
     1681 cgcgtttaac acttggtatt gccaaaaatc taggttggct taatttttca gattcttatc
     1741 tgagtctcta cggattcaca gccttactta tttatctagc gtattttatt ttattgacga
     1801 aattcaatca tggccaaacg attgggaaaa tgatctttgg tattcgagtc atcagctttg
     1861 atgaagaaga acttagctgg tcaaccgttt tagttagaga aggtgcttgc cggttcattc
     1921 taaaatttcc tttgctgatg ttagggtatc ttccagcagt ctttaatcag aaaaagcaac
     1981 atctgggcga ttttttcact aatacaagtg ttgttacgat caatctgatc aaagcgttca
     2041 accaggaagt caacagctga tgagcaataa aaagcagtaa cagttgaaag caccttctaa
     2101 aagtcagatc taaaatctag cttttagagg gtgcttcagt tatttttaat tgtcatgctt
     2161 tttttgtttt gctactattg aacatgagct gaatatgagc aagaggagga cactgcgtga
     2221 agccgattga ttttccagaa aaatatgaga atttaatgcg agtggcacaa caagcactag
     2281 caaaccagca atatcagcag gcaaaagaat tatttcaacg agcttacgag ttgaaagaaa
     2341 gctttgaggc taatagcctg cttgtttttt gtctttacga gttggacgag aaaaaagaag
     2401 cattgaagca agcactcttg catgaaaaac agtatcttca aaatgaggag tttgctgaat
     2461 tttattttga cttgttaatc agtacacaag attttttata tgctagaaaa ttgatagcaa
     2521 gtacagattt ttatgaagct ttcgagcagc gggtcattga aaaaatccaa tttgcagaag
     2581 aattatccgg acaaatggag aggcagaaga tcaaggcact ccacaataaa tcggaggaac
     2641 ttccttcgtt ggaacctgca cgtcagcttg cgtctattga tgagatcgaa cagcttcctt
     2701 atcatgagtt tatccaaaca gcgagtaaac ttattgtatc gccagaggtc catattttag
     2761 caagggcaaa attactggag acacttaggc aattgaacga atgccatcct gttttctatt
     2821 tgacgattga agaaaagtta gtcaaagtca taccaaaaga tctgccaaag ccgcagcagc
     2881 aggcaagtta ccagcaatta tgtgtatttt cagatcgtta cgggaatgaa gatgcgctgc
     2941 ttagttccgt gttaaaagaa gaattcacgt tgcaatctgc aattgtttat cctgtctatg
     3001 atacatacat cgaagacccg aagcgctggt ttcaactgac tgtagaggcg tgtacaggaa
     3061 aaacgatgta tgaaggtaca gaagatgaaa aacaagattt tttaaaaaaa agagaaaaaa
     3121 ttttgcagca aatgatcttc ttccattaga ttttatcagt gaaaaagata aaaatgttat
     3181 gaaaaac
//
LOCUS       DAHUWM010000240         3168 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHUWM010000240 DAHUWM010000000
VERSION     DAHUWM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.245"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(115..612)
                     /locus_tag="NK788_002580"
     CDS             complement(115..612)
                     /locus_tag="NK788_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCH6770876.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(742..1452)
                     /gene="budA"
                     /locus_tag="NK788_002581"
     CDS             complement(742..1452)
                     /gene="budA"
                     /locus_tag="NK788_002581"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCH6770877.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1465..3129)
                     /gene="alsS"
                     /locus_tag="NK788_002582"
     CDS             complement(1465..3129)
                     /gene="alsS"
                     /locus_tag="NK788_002582"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCH6770878.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          876 a          703 c          521 g         1068 t
ORIGIN      
        1 aataacactt gtgattttat aaaaaaataa aaaaaccgaa aaacagaaat gaccactgtc
       61 tttccggctc tttttattct tgatttctct tcttttttgt ttcaatagac gattttagtt
      121 attaataaat gaaaaagcat ttatattaaa atttgctaat aaaagatcta catatttttc
      181 tagaaatgct tgatcgattt catcataccc tttcgttact ccgcaatcga tatctaaaac
      241 accgattaaa ttaccttctt ttaccattgg cactacgatt tctgattgtg cggctgaatc
      301 gcaggaaatg taatttgcat gggttttgac atcatcaacg atcaatgttt cttgtttttc
      361 agctgattct ccacaaactc cttttcccat cttgatacgt gtgcaagaaa ctcgcccttg
      421 gaacggtcct aaaatcaatt cattaccatc gaataaataa tatcctgcaa agacagtctc
      481 aggcaaagtc tcattcaaca atgcagatga atttgctaaa tttgctaacc agttatcttc
      541 tgcttctaat aatccttgtt gctgtaataa taaaatttcg tatgcatctt ttttctgttt
      601 ttctgtccac atgataaaaa ccgctccttt ttgatctatg gagaagatta tagctcagtt
      661 tttttgtaaa cgctaggttt cctcctcaaa aaaacgagat acgtcaggtt aatcaccttg
      721 aacgcatctc gctgtctttt tctattctgc ttgttggatt tctgctgcta aatcagcata
      781 atcaatcgtt tcgtgaacaa aagtcggatc atcaactgga aaatgctgta caagggacga
      841 gatgcggcta atctcaactt ttccttcagc cacttcaaaa tccattacat gtccgccaaa
      901 tgtatgtgta tcatcgataa aatgcaggtg aaatcccgct gcagcaactc cttggaataa
      961 ttcaggcgta aagaacccga ctaatgttcc ttggatattg ctttgttgga attctggttg
     1021 agactctgaa atttttgcta gtcttctgta aggtttttct tgcttcggca tgatacggat
     1081 gtgcatattt ttgaataatc cagttatctt cactgccaaa aataggtttt ccccggcttc
     1141 tcttactaat atgtcttttt tcaattgttc tccatctgca gtttttgaga cagaaaaact
     1201 agtatctggc gtaaaatctg taattgctgc atagggggta agttcacttc catctaattg
     1261 ggcaactgag ccatctgatc ttccttgata agctacgcca tctaaaaaga tgacttcacc
     1321 atcaagacca tgcaatgttc ccaatcccag agttcctttt tctaatatat gagcaatcgt
     1381 ttctgttcca tccatcaagc cggccatcaa agctcctaat gtcccgtgct ggtataagat
     1441 tttttcagtc atgtcgacac ttccttaata gaattgatct ggtaagattg tttctcccaa
     1501 tttctcatta tcacggtaat caatcggaat atcaataatt acgggtcctt ctgtcgcaaa
     1561 tccttcttct aaagcttttt ctaattcctc tacacttgta gctcggattc ctttagcgcc
     1621 aaaagcttct gcatatttca caaaatcaac tggaccaaag tctacacctg acgcccgctg
     1681 atatttcatt ttttcttgga actctaccat attgtagtgt ccatcattcc aaatcaaatg
     1741 aacgatattc aattttttac gtaccgccgt ttctaaatcc tgggcagaga ataaaaatcc
     1801 cccatcgccg gaaactgata cgatttgtgt atttggacgc accaatgcag cagaaatcgc
     1861 ccatggcaat gcaacaccaa gcgtttgcat accattactg aataacaaat gacgaggttc
     1921 ataacttcta aagtggcgtg ccatccaaat ataatgacta cctacatcta cggtaacagt
     1981 catatcatct gttacttttg actgaagcgt gttaataact tctaaaggat gcaagatacc
     2041 tgcttctcct tttgattgaa caatgtcccg ctccgttaat tttgcttgaa gagatgctaa
     2101 atattctttt gcatcttctg acacttgttg aggctctaat gaacctgtca ataaatcaag
     2161 agtggctgag atatcaccga tcaattctct ttcaggctgc ataaaaggat cgatttcagc
     2221 tggcgcttca tcgatcacaa tgatccgcgc atctttttcc gcattccagt ttcttgcttc
     2281 atattcgatt ggatcatagc caattgcaat aactagatca cttcgtttca aaagcatatc
     2341 tcctggttgg tttctaaata aacccacacg accaaagaaa tgcgcttcta attcacgtga
     2401 gatcactcca gctgcttgga atgtctctac aacaggcaat gctgtctttt caactaattg
     2461 cctgatggct aacgtttctt tttcacttga tcctcgcata cctactaata gtaccggtag
     2521 ttttgcttct ctaatgcgtt cagctaggta acggatatca tctgcagaag cagaacctaa
     2581 tttaggatcg ctcaatggct tgatgacatt accttggaca ggtgcatcca ctacatcttg
     2641 cgggatactg ataaagctcg cgcctttttt tgaactctta gccatacgat aagcattggc
     2701 aatcacttct gaaattgttt ctggatcttg gatttctgca ctgtattttg tgattggctg
     2761 gaacagtgct gcattatcca tgctttgatg ggtcaacttc aatagatcgc ttcgttttac
     2821 ttgtccggca attgctaaaa ctgggtcacc ttctgctgtc gctgttacca aacctgtagc
     2881 taagttacta gcaccaggac cacttgttgc gataactact cctggctctc ctgtaatacg
     2941 tccgattgct tgtgccataa atgcagcatt ttgttcgtga cgagtcacga ttaattcagg
     3001 accttgatcc tctaattcat taaatacacc atcgattttt gcacctggta taccgaatac
     3061 ataatcaaca tgatgattaa tcaaactttc tactacagat ttcgaacctt gtttcatttt
     3121 ttcagccatt gaacatcgtc ctttttatgc tttattttta attattag
//
LOCUS       DAHUWM010000241         3166 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHUWM010000241 DAHUWM010000000
VERSION     DAHUWM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.246"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            35..247
                     /locus_tag="NK788_002583"
     CDS             35..247
                     /locus_tag="NK788_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770879.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(525..2324)
                     /locus_tag="NK788_002584"
     CDS             complement(525..2324)
                     /locus_tag="NK788_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCH6770880.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2448..3044)
                     /locus_tag="NK788_002585"
     CDS             complement(2448..3044)
                     /locus_tag="NK788_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCH6770881.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1129 a          582 c          570 g          885 t
ORIGIN      
        1 aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag taggaagatc
       61 ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg aacgtacata
      121 tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc aacttatcaa
      181 tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg ctgaattggt
      241 ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa taaagacaga
      301 agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat tattcttgca
      361 gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga atcaagagaa
      421 agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt ggcaggttat
      481 gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc gactcccaaa
      541 acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt cacactttca
      601 tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat catgcgactc
      661 atagcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg gtgggcagaa
      721 ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc aggcgtcaca
      781 gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg ttctgcttct
      841 tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt ctctaagata
      901 actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt tttagaggtc
      961 ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata agaatcaaaa
     1021 gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa ttctttcaat
     1081 gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc atcatgcatc
     1141 actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc aggatgatag
     1201 tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg ggtactcaca
     1261 cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata atccatattg
     1321 taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt gtttttcagc
     1381 gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc aggcaagaag
     1441 ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga catcacgata
     1501 ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat caatgcatag
     1561 tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata tcctaaagaa
     1621 gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc caaaagacgc
     1681 gatttggtca atgaccagct ttcccgaatg gctgctcgaa aaggacgatc tcgtagaatc
     1741 atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat ataaacgagt
     1801 aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat gaaagcgggg
     1861 atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg tagaatcaat
     1921 aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag ctgaagtatc
     1981 gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa aaaaacactc
     2041 atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa aactacgatt
     2101 aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc ataagacaaa
     2161 tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa gggtaaacaa
     2221 ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg tgttcctttt
     2281 aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc acctgctctt
     2341 cacattgtag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg cagacccttt
     2401 cccagattta acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga
     2461 ccacttagat attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt
     2521 ttagcaatca aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc
     2581 aaacataacg catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca
     2641 aaacggtttt gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg
     2701 gaaagtatga atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg
     2761 cagattccaa tatagttata gataaatcca gcataagggc caaatatcag gacaccagct
     2821 gcagtactga tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca
     2881 aaaataatcg gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct
     2941 ttgaaaacac ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata
     3001 atggatatga aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt
     3061 agaggttctt tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata
     3121 tgacagaaaa caaacaattt tgtaagttca tcataagtat aaaaga
//
LOCUS       DAHUWM010000242         3142 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHUWM010000242 DAHUWM010000000
VERSION     DAHUWM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.247"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..153)
                     /locus_tag="NK788_002586"
     CDS             complement(<1..153)
                     /locus_tag="NK788_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6770882.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            1030..1284
                     /locus_tag="NK788_002587"
                     /pseudo
     CDS             1030..1284
                     /locus_tag="NK788_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1354..1866
                     /locus_tag="NK788_002588"
     CDS             1354..1866
                     /locus_tag="NK788_002588"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6770883.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1863..2900
                     /locus_tag="NK788_002589"
     CDS             1863..2900
                     /locus_tag="NK788_002589"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6770884.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3060..3142
                     /locus_tag="NK788_002590"
                     /pseudo
     CDS             3060..3142
                     /locus_tag="NK788_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1125 a          564 c          610 g          843 t
ORIGIN      
        1 cacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt tattgatgac
      241 cacgaaagtg catggcgata tttgatgttg atactttatg cagtacccta tgtattcttc
      301 gctatcattc tgtggctcat agcattgttt tttagagtat ttaatgacct ttaggtagat
      361 ttatatataa aaagggctaa atacgcaaaa taccccctta aaatgcaaaa taaatgcaaa
      421 taagggggta actccttatg aaaaaacaac taatagtatt ataacacgtt tttatgccga
      481 gaaaacttat tggttggaat gggctatgtg ttagctaact tgttagcgag ttggttggac
      541 ttgaattggg attaatccca agaaagtacc aactcaacaa cacataaagc cctgtaggtt
      601 ccgaccaata aggaaattgg aataaagcaa cgaaaggagt tgagtaaaat gagtgagaaa
      661 acgaagaaaa tcattcgagt atcattagct ttttcacaag tagtagtgca atcattgata
      721 gtttattata cttaccagca atataaatta tctaaagaga attaagtttt gattatgtaa
      781 taagttttgg tactaaaaaa gacttgatct aattagacca agtcttttgt tagtgttata
      841 ttaataacaa aataaaaata agaagtcgct cactccccga ccaaagtttg tgagcaaaat
      901 aaacagtcga tgtttgtttc ttaattgtat atcttgatat taaacgattt aagattattc
      961 ttcaagatat atattcgcaa atgtaattgg aaaagcgact tcttaaacaa aattaaggag
     1021 tcgcttttta tggcaaaaat aacgaaaaat gagcaagaaa atttggtgga aaattacaca
     1081 aaaaaaccgg cgtactctaa tagccggtta gacgcacatt tcgtgtgcac ttctaatccg
     1141 aaattgagct ttgacgctat gacgatagtc ggaaacctta ataaaaacag cgctaaaaag
     1201 ctatccgatt ttatgagttt agatccacaa attagacttt gggatatact tcaaacaaaa
     1261 tttaaagcta aagcactaca agacccccta tgttaggcta gttgtcgtat aggataataa
     1321 gttataataa agactatacg gaggatgatt accatgacaa ataaaaacaa tgaacgttac
     1381 tctaccgaac tgaaagcgac agccatcaag aaaatgatgc cacccgaaaa tagggggatt
     1441 cgccaattaa gtcaaacttt gggcattcca gaagcgactc tttattcttg gcgtaaaaaa
     1501 gctagaattc aagggaatac tacacccgga acgaaaaaaa atgtcgaaaa atggtcaagt
     1561 gaagataaat ttttaattgt gatggaatca ttctcaatga accaagtaga acttgcggaa
     1621 tattgccgaa agaagggctt atttaaagaa caaattgagg cttggaagaa aacgtgctta
     1681 tctgcgaatg aacaggaaga aaatcgaacg agagaacttg caactgaact aaaagaagag
     1741 aaaaaacaag cgcgtcaatt agagaaagaa ttacgtcata aggaaaaagc attagcggaa
     1801 gccgctgctc tcttattact aagaaaaaaa gcacacgcga tttgggggga ccaagaggaa
     1861 gaatgatttc atcttcagat cgcaaacaag ctgttgaact tattcaagaa gccaatcgca
     1921 atggggcccg tctcacctat gcctgtaatg agctgaatat tagcgttcga acctacgaac
     1981 gatggacgcg tgaaggtacg atagcgcatg atcaacgccc tttggcgaaa cgtcctgtac
     2041 ccaaaaataa actgacggat caagaacgcg aaaaaataat agaaaccgtt tcaaaaaaag
     2101 aatttatgaa tttgccacct agccaaattg taccaaaact tgcggattgc tcgatttata
     2161 ttgcatcaga gtctagcttc tatcgtgtat tgcgagaaaa aaatatgcaa catcaccgtg
     2221 gccgaagtca agtttcacaa aaacgcatac cgccaagtca ccttgcgacg aagccaaatg
     2281 aagtttggac ttgggatatt acttggctaa aaggtccaat aaaaggactc ttttaccggt
     2341 tatatctcat cattgattta tttagccgta aaatggtagg ctgggagatt tgggaatcag
     2401 aagaagctcg tcatgccgaa aaacttatta aacagacggt gcttaaggaa cgtatccaag
     2461 gacagccgtt ggttttacac tccgataatg gcagccctat gaaagccgcg acttttcaag
     2521 tcttactcga aaaattagga attcaaagtt cttattcaag accacgagtg agtaatgaca
     2581 atccttattc ggaagcggct ttccgaacgt taaaatatcg accagaatat cccaataacg
     2641 gctttaaaac aatagatgat gctcgaaaat ggacgaaaga gtttgtcgat tggtataacg
     2701 attctcattt gcatagtggg attcaatttg tgtctcctgc agcgtgtcat gaaggacgac
     2761 atctcgctat tttagagaaa cgaaagcagg tttacgagga cgctaaaaag cgccatcctg
     2821 aacgctggtc aaaaggtgtt cgaaattgga ctccacatca atcggtggcg ttgaatcctt
     2881 taaaagaaac agaaagttaa caggagcata atctttcaag attatcaaat aaccctcgtc
     2941 acccaatagg ggaaaaagtt attcttcccc tattggataa cgaggccaac cgaccatagg
     3001 gaggttctgc tttttgatag gtggaaaata aaaatacgac aactatattg acaaacaccg
     3061 accatcgtcc agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacaa
     3121 cgattaaagg catggaagcc at
//
LOCUS       DAHUWM010000243         3092 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHUWM010000243 DAHUWM010000000
VERSION     DAHUWM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.248"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(65..628)
                     /locus_tag="NK788_002591"
     CDS             complement(65..628)
                     /locus_tag="NK788_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCH6770885.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(643..1686)
                     /locus_tag="NK788_002592"
     CDS             complement(643..1686)
                     /locus_tag="NK788_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770886.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1819..2223)
                     /locus_tag="NK788_002593"
     CDS             complement(1819..2223)
                     /locus_tag="NK788_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCH6770887.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2456..3091)
                     /locus_tag="NK788_002594"
     CDS             complement(2456..3091)
                     /locus_tag="NK788_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCH6770888.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT          943 a          648 c          530 g          971 t
ORIGIN      
        1 ccataaaata taaataatag acctagcatc atctggtata aaatggatgc taggtccgtt
       61 atatttactc aaaattgatt ctacgaatag atcttcttat catttctatt ttagtagtca
      121 tgcgttgttg ttcttctaaa ggtggtaatg gtataagtag tttagataaa tttgtaccat
      181 ttacatttgg ttgcccagtt ccgtaagaaa ttttttctaa taacttccaa tataatggcg
      241 agtctaagaa gcagtctacg tattctgata ataatttctc aactaaccta attcgaatca
      301 agtaagaagc aaaaacagat tcctcactta tttccttaac caagaaactt tttcccatag
      361 ttcctcctgt ccttgctatc aaaatgtcat tttcttccaa tcttaaatca actagtttag
      421 aattactaat atcacagtaa ggcacactgg accaatttac acgtccctct tgtatatctg
      481 tgattctcaa aaacttaaca ttgccttgtt ttttcgccga atctgtatag ccatattgaa
      541 tcttagtaga aatacttcct aaataaaccc atagtcgaac atcgctagat gatttttctt
      601 gtcgttcttt atgaacaaca gtgatcatct tgagttgtcc gactattttt tagttgcagc
      661 aatctttttg ggacgcttat ttgcatgaag caacgttgct aagtctagta ataaaacatt
      721 ggctaacaca ggattggctt tctcgcgata cgctgcggcg ccactttcaa acaatactgc
      781 tagacgatct tctacaacaa cgatttgttc caaatacggt ggaaatttga ttcgttttag
      841 gatccggcta ggagagtccc gctcggacac taagagttcc gaggtataag gaccataaga
      901 acgactggct aagagataat ggccataaaa agccacacct tgaatcttat cagatgtttt
      961 tctgctcatt gtcacggttg gttggatttt gtctttgcgg cgaattgctg ttttcatttc
     1021 agctatcgtc ggcaatagat agaaagtcaa ctgtccttca ccttttagtg tgaagttgcc
     1081 agataccagt tgatcattgt tttgagtcaa atacgatgct tgtggaatct ctgagagatc
     1141 agttgtcagc caataagcga ttggctgtcc cgtctcttca gatgtatctg ctaagatatc
     1201 gatcaaacgt aaagcggcta ctcgtccagt agcagatcct gtgattgtca accataaaag
     1261 ttcccgtttt gtatcatata ctaagccccc tacatgtggc cgtttaggta aaatcagcag
     1321 tttgatcggc tgtcctgttt ctctatccag tacatgaata atagaatgat gctgatggtc
     1381 atagcagtag gcagaaataa acacaaaatc ttgagaaaaa gccaaccctt gcggcgtcat
     1441 ccattgacaa acctcatttt cgccggtttc cggcaccacc attcttgttt ctgttaatcc
     1501 aggaatgtta tattgttttg cttcagcctt ttcccaagct gcttgaacaa taggataccg
     1561 ttttaacttt ttttcaatat cctgatgtat ccgttgctgc ttccaatgct taaactttag
     1621 gacatagtaa acaagaaggc caatgaccag caacactaaa atcattattg ctacccgctg
     1681 tgtcattttt ttgcctcctt gttctattcg atcaatcgtc catataccgc tattaattac
     1741 cgtaactaca tgtttctttt actagcgttt ctttatagga tctttcataa tctcatttat
     1801 agtaacatta agaatccctt attctactat agtcacttcc acatcaatat tgcctcttgt
     1861 tgctttagag tatgggcaaa atgcgtgggc ttcttctgcg attttctctg cttggtcctt
     1921 ctcaagtcct ggcaaagaaa cttctaaaac gaccccgatt tggtagcccc catcttctaa
     1981 ttgaaataaa gatacccgtg ctttgacttc agaatcagac tcaagattat tttcttccag
     2041 catgtgttga accgcgccgt tgaagcaaga tgcgtatcca gccgcaaaaa gttgttccgg
     2101 atttgtccct tcactatgga ttcctgggcg gtcgatcttc atgtgcatac ttccgtttgg
     2161 tgcctcaact tcgccatcac ggcctccacg attgatcatt gctgtttcgt acattttttt
     2221 cattgagatc atcctttctt tcgtcgttct atattaagtg taacgagtat tttttaatct
     2281 gacaaaggta atgcttgcat tcggcgacat cagttagaca acatcgatga tcagtgatag
     2341 aagcagttta tctatacaaa aaaagaaggg ttctatgata tttggtgtcc ttactcgcct
     2401 tgagtaacaa caccaaatat catagaacca cttaatcagc ttcacactgc tttttctact
     2461 ttcgtagttg aaaaaaactt aaacaaatca tcggcaaata gttaaagaaa aaacttaatc
     2521 gttgaaagaa gcccaattgc ttaaagaagg caaactgact aatcgacgga atccgtgcta
     2581 atccgtaaag acctgcaatt agtaaactaa taactgttaa aacaagataa aaatggctaa
     2641 gcgtaccttg accactttga cgataaagca gaacaagtaa caatgggaaa agtagaaagc
     2701 ctgcatagcc tagcccagaa cctatattat ggatccaagt cgatagatta caagagcttt
     2761 cattcgtatt gatactgaat aagccagtaa aaatacaatc acctatccca tataaaccaa
     2821 ctgttgcaac aacaagtatt gcaagtgttt ttgatgttcc aacaaatagg tgatacaacg
     2881 caggcaacga aagaacaaaa aaaagtccag atacaactga ccagaccaag aaagcacgtc
     2941 tgactggact atctacgtca ccaaacacac taattacggt agtcatttga ttcagtttgg
     3001 gataaaaaag gccaaggata tacggtgtcg ataaatcact catcacacct aataataaaa
     3061 aataaaaacc atatttttgc aaaaaaatca tt
//
LOCUS       DAHUWM010000244         3058 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHUWM010000244 DAHUWM010000000
VERSION     DAHUWM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.249"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            28..783
                     /gene="fabI"
                     /locus_tag="NK788_002595"
     CDS             28..783
                     /gene="fabI"
                     /locus_tag="NK788_002595"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCH6770889.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(893..1906)
                     /locus_tag="NK788_002596"
     CDS             complement(893..1906)
                     /locus_tag="NK788_002596"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCH6770890.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2071..2991
                     /locus_tag="NK788_002597"
     CDS             2071..2991
                     /locus_tag="NK788_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCH6770891.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          889 a          618 c          588 g          963 t
ORIGIN      
        1 ttttatcaga aacaaaggag agtcattatg tcatttctag aaggaaaaaa aatcgtcatt
       61 atgggcgtcg ctaacaaaaa aagcattgct tggggttgtg ctgaagcaat gttgaaacaa
      121 ggggcagaaa ttatctatac ttaccaaaat gaacgaatga aaaaatcatt agtcaaattg
      181 gtcggcgaag aagcattcac tgtagaatgt gatgtttcta gcgacgaaag catcgaaaaa
      241 gcaatgaatg ccatcggtga atatgctggg gagatccacg gccttgtcca tgcagttgct
      301 tacgccaaca aagaagaatt gtccggaaat gtgtctgata tttccagaga cggctatcaa
      361 ttagctcaag atatcagcag ctactcttta attgctgtat caaaatacgc acaaccgttt
      421 cttgcaccaa acagcgggat cgttagcatg acttatctag gttcagaacg cgcaatcccg
      481 aattataaca tgatgggtat tgcgaaagct tctcttgaag ctgctatccg ttatttggca
      541 gctgagttct cgcctaaagg aattcgggtc aatggaatct cagctggggc aatcaaaaca
      601 ttagctgtca caggtgttaa agactatcaa aaactgatcc agttatctga atctcgtacg
      661 cctgatcagc aaggtgtgac gatcgaagaa gtcggtaatg cttgtgcttt cttaatcagc
      721 ccactttcca gcggaatcat cggtgatatt atctacgttg acaaaggtgt ccatctttca
      781 taaacttttg ttgcattctt taaagtaaaa catataaaaa tcaggaaaaa actttttaaa
      841 gtctcttcct gattttttct ttttacgttt ttttcaactc attactttga aattattcca
      901 cgacttcaat ccagccttct ggtgcttcta tgtctccaaa ttgaatacct gtcaattcat
      961 catacaaccg ttttgttact ggtcctactt ccgtttcact ataaaataca tgaagattgt
     1021 tattgtgata aatcccaccg attggagaaa taacagccgc tgtaccacat gcaccagctt
     1081 ctgcaaattg atctagatca tcaatgaaaa tatctccttc aattgcttgc agtcctagtc
     1141 gttcttcagc taactgcaac aaggaatatt tcgtgatact tggtaagata gagggcgatt
     1201 gcggagtgat gaattttccg tcttttgtaa ttccaaagaa atttgctgca cctacttctt
     1261 cgattttcgt atgtgtggct ggatcaagat aaacacaatc tgagtaatcc agttcttttg
     1321 cttctgttcc tggaagtaaa ctcgctgcgt aatttccgcc gacttttgct gcaccagtcc
     1381 ccttaggagc agcacgatcg taatcagata caataaaatt cgttggtttt agtccacctt
     1441 tgaaatacgc tcctactggc gtacagaaga cgacaaataa atattctgga gctggatgga
     1501 caccaatgtt ttcaccgacc ccaatcagta aagggcgcaa gtataatgta gcgcctgttc
     1561 catagggcgg cacaaaatct tcatttgctt ttaccacttg cttgacagca tcaacgaaca
     1621 tcttttcagg gaccattggc atcagcaatc gtctagcact ttgattcatc cgtcgactat
     1681 tttgttcagg tcgaaagaga ttgaccttgc cgtcttttcg acgataagct tttagacctt
     1741 caaaacattg ctgaccataa tgaagcgctg gagagccttc atgaatcgtc aggtaattat
     1801 cttctgtcaa ttctccttct tcccattgtc cgtctttcca tttggcaata aaacgccaag
     1861 gagttttgat ataagaaaaa cctagattgt tccaatcgat ttgcatatac ttaccacctt
     1921 cgttattttc tccacatttt atcattatcc atattatttg tcattttatt ttcagctttt
     1981 ttccttcatt atagttattt ttgctatttc cttttttgaa aacttcttgg tacaatacag
     2041 aaagaatcaa attttagaaa agaggatttt atgttttcat tattagcaac tgccggtacg
     2101 acagactctt ctggcgagac attgacagat caagctgtca gcaaagtaaa tgcgtttcag
     2161 cggttttggg acgggattga ttgggacgcc attttggctg cctttatcca aaaaagttta
     2221 tatctgattt ttttgatcgt ccttttttgg attcttcacc gtgtgggaaa atatctgatt
     2281 gataagtctt ttaatcagta ttctaaaaaa gccattctaa acgagagccg cttaaagact
     2341 ttacatagtc ttttaaatac tatttttcat tatacccttg gtttcttttt tatctatgca
     2401 attttatctg ctatcggtgt gccgatcgga tcacttttgg ctggtgccgg aattgcaggt
     2461 gtggcaatcg gtcttggtgc ccaagggttc atgagtgatg ttattaccgg attttttatt
     2521 atcatggaac aacagatgga tgttggggac tacatcaagc tagcaaactt aacgattgaa
     2581 ggaaccgtcg tatctgttgg tatccgtacc cttcagctga aagcaacgga tggtactgtc
     2641 catttcatcc cgaatcgaaa catcaccacg atcagcaatc tctctcgtgc aaatatgcaa
     2701 gtacttttag atattcggat tgttcctgaa gaaggatatg acaaaatcta tgagatcatt
     2761 gatcgagtaa atcgtacatt agctgaaaaa taccaagaag agctccaaac tgaaccttct
     2821 atctttggat tagtggatct tggaaacagc aattttgcga ttcgtacggt ctgctacgca
     2881 ttgaacggaa aacaatatgc cttaaaagaa gagttcttga gctcctatgt caaagaattg
     2941 acagcttctg gtttcacgat tccgaacagt ccaattgctg tcggaaaata atttggacaa
     3001 acgatgcaac gaatctcact aaaaagagcg tgggacaaaa gtaaagaaca cttttgtc
//
LOCUS       DAHUWM010000245         3024 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHUWM010000245 DAHUWM010000000
VERSION     DAHUWM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.250"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1243
                     /locus_tag="NK788_002598"
     CDS             <1..1243
                     /locus_tag="NK788_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6770892.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETSFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="NK788_002599"
     CDS             1256..1888
                     /locus_tag="NK788_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCH6770893.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="NK788_002600"
     CDS             2093..2320
                     /locus_tag="NK788_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCH6770894.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="NK788_002601"
     CDS             2389..2655
                     /locus_tag="NK788_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCH6770895.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="NK788_002602"
     CDS             2645..2914
                     /locus_tag="NK788_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCH6770896.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          442 c          607 g          903 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagacttct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DAHUWM010000246         3013 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHUWM010000246 DAHUWM010000000
VERSION     DAHUWM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.251"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            177..359
                     /locus_tag="NK788_002603"
     CDS             177..359
                     /locus_tag="NK788_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCH6770897.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="NK788_002604"
     CDS             462..746
                     /locus_tag="NK788_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770898.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="NK788_002605"
     CDS             762..998
                     /locus_tag="NK788_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770899.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="NK788_002606"
     CDS             1020..1274
                     /locus_tag="NK788_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770900.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="NK788_002607"
     CDS             1356..1541
                     /locus_tag="NK788_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCH6770901.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="NK788_002608"
     CDS             1608..2003
                     /locus_tag="NK788_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCH6770902.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="NK788_002609"
     CDS             1993..2370
                     /locus_tag="NK788_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770903.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="NK788_002610"
     CDS             2392..2766
                     /locus_tag="NK788_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770904.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DAHUWM010000247         3012 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHUWM010000247 DAHUWM010000000
VERSION     DAHUWM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.252"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            202..1077
                     /locus_tag="NK788_002611"
     CDS             202..1077
                     /locus_tag="NK788_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770905.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="NK788_002612"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="NK788_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="NK788_002613"
     CDS             complement(1642..2973)
                     /locus_tag="NK788_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770906.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1060 a          544 c          466 g          942 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tt
//
LOCUS       DAHUWM010000248         2966 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHUWM010000248 DAHUWM010000000
VERSION     DAHUWM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.253"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..296
                     /locus_tag="NK788_002614"
     CDS             <1..296
                     /locus_tag="NK788_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6770907.1"
                     /translation="DCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(382..1476)
                     /locus_tag="NK788_002615"
     CDS             complement(382..1476)
                     /locus_tag="NK788_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCH6770908.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1665..2426
                     /locus_tag="NK788_002616"
     CDS             1665..2426
                     /locus_tag="NK788_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCH6770909.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2541..>2966)
                     /gene="fabZ"
                     /locus_tag="NK788_002617"
     CDS             complement(2541..>2966)
                     /gene="fabZ"
                     /locus_tag="NK788_002617"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCH6770910.1"
                     /translation="SATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTINEDFFQ
                     GHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVPGDVLK
                     CHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          950 a          588 c          508 g          920 t
ORIGIN      
        1 ttgactgtgg aaaagaattt tctaactgga aagccattag taaccaacat gatattgata
       61 tatattttgc ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga
      121 ttctgcgtcg taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta
      181 tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac
      241 caatcgaaat atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa
      301 tcataaccta taaaaaaaca aaataaaaaa gcagaaataa ctggctattt ctagcggatt
      361 atttctgcaa taactaatta tctatttttt attttcaatt aatttaaagc ttgaccatgg
      421 tttcaaaaag tcaaggagga ataattcgtc atcggagatt cttcctacaa cattgacacg
      481 tccgtcgttt ttcatatcct tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc
      541 ttcattgtca atcagtacat ccccatgatg catatctcgc gtattatgag gcgggaaatc
      601 tttatctttg tagtatacac gagtcatcgt tgaacgaagg atatattctg agcgatcccc
      661 tcgataacta tgaaggttat caaacaagca gattcgttcg ttttctgtaa taccttcttc
      721 cgtatctact ttcaatgttg gataatccgc gttaaatgct tctgccattt ctttcaattc
      781 ttcttcactt gcgtacgcat ttccaataga aatatcatca atcaatcctg taaggattaa
      841 atgcttcact tgtgtcgcaa tttcaagatc tcggtggtct tccagcgtgc acaagccatc
      901 ttgcgtaggc caagggccaa actcagcaga ttgcgaattg acgaatgcca tcgtattcaa
      961 attatatttt ctgaattttt ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta
     1021 tcgatgagga taaaaattat gagaacctaa taaattatcc gtgtttggcg aataactcat
     1081 gatgttgtca acgtaattcg tgcccgaact catattgatc tcgattttga taccaaaagg
     1141 attgcgcgtc atttttgctt cttctgctcc tgtgaatccg atatctaagc ggaccccgta
     1201 ggcacccatc ttatggaaaa aagataagtc atcgtaagaa atctccaact gttcaaataa
     1261 cgctggattg atatcaacca ttacttccat gcctaggcta ttcgcgtaat caactacttc
     1321 tttaaattct gaaagaactt tttctttatc atcattgatc tgcagcaaac tggtaaaaac
     1381 ccgtttatac ccatacttat gagctaaatc caagtacgct ttgtcctttt caaatgttga
     1441 ccgttctgga taaatcgaga ttcctaattt tcccatatta ccatttcctc ctgcatcttt
     1501 tcgtttttgt atccacttac attatagcaa acacaaagta aatggtctac accttttctt
     1561 tttcttttaa aataaatgat aaattccaat aatttaagta ggtattttcc ctctattttg
     1621 ttatcattaa agataacaaa atagtaaatt ggaaaggaac agttatgaag aaaaataaag
     1681 ttattttaag tattagttta gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca
     1741 acacgacttc ttctagttcg tctacagaaa gttcaaaaag tacatctact gcggaaagca
     1801 cgactgccag tagcgtcgat ttgaacgcat tggaattacc acaattgacg aaagatgtcg
     1861 ccgaaaatga agatctggtc gaaatggtga cgaataaagg cacaatcaaa atcaaattat
     1921 ttcccgaaca cgcaccaaaa gcggttgaaa actttatgac acatgcaaaa gacggttact
     1981 acaacggact gactttccat cgcgtcatca aagatttcat gatccaaggt ggcgatccta
     2041 aaggcgatgg cactggaggg gaaagcattt ggggagaagg atttgaaaca gagatcaaca
     2101 accaccttta taatataaga ggtgcgttat ctatggctcg ctcacaagat cctaacagta
     2161 acggcagcca gttctttatc gttcaaaata cagatgatat gcatgatggt cttttaaaag
     2221 acgactatcc tcaagcaatc atcgacgctt ataaaaacgg cggttatcct tcactagatg
     2281 gccattatac tgtcttcggc caagtgattg aaggaatgga tgtcgtggat gctattgcaa
     2341 atatcgaaac agatagttca gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata
     2401 tactgcaaga agcaaaacaa aaataaaaaa ataacgtctg aatcctgcat agatgacaac
     2461 gtatgctagg attcaggcgt cttcttttta cataaaaagc aagtaagctt ccttttgttt
     2521 tgctatatca ggttttatag tcaagcattt tggttgacga tgaacgtgaa ctcgcactca
     2581 cagacttttt tatcatctac atatgctgca gataacgctg taccgactga tcccttcatt
     2641 ttggtgattt caaaatgaca tttcagcaca tctcctggaa caactttttg acggaatttg
     2701 gccttattga tcccaccgat ataagctctt tttcctagaa actcttctga ttttaggata
     2761 agtatcgaac ctgcttgtgc cagtgtttct agaatcaacg cacccggcat cactggattg
     2821 tcaggaaagt gaccttggaa aaaatcttca ttgattgtta cattttttgt cgcaacgatc
     2881 ttttttcctg gttctaaagt atctacataa tcaatataac aaataggata acgattagga
     2941 atcaattcca ttacttcagt agcaga
//
LOCUS       DAHUWM010000249         2909 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHUWM010000249 DAHUWM010000000
VERSION     DAHUWM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.255"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            59..202
                     /locus_tag="NK788_002618"
                     /pseudo
     CDS             59..202
                     /locus_tag="NK788_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            352..552
                     /locus_tag="NK788_002619"
     CDS             352..552
                     /locus_tag="NK788_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6770911.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(806..2485)
                     /locus_tag="NK788_002620"
     CDS             complement(806..2485)
                     /locus_tag="NK788_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCH6770912.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(2487..2699)
                     /locus_tag="NK788_002621"
     CDS             complement(2487..2699)
                     /locus_tag="NK788_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCH6770913.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT          857 a          564 c          581 g          907 t
ORIGIN      
        1 tcaaagtgaa agtattgatt taaatgattc ttttgtaatt tactcttgat aatatgaaat
       61 tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag aaatgatggg
      121 gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt tgagagaaga
      181 aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat
      241 tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc tatcgtacac
      301 gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat tatggaaaac
      361 ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg tgaagatgga
      421 agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac attagaagaa
      481 ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt
      541 gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt ttacgagaga
      601 acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg caggtatttt
      661 tttgtatgaa aaagtaaata aatttaagag gctatgaaag agccaccttg tatcaaggaa
      721 taagaacaaa caaaaatggc ttctcatgtt tttcatttgt tttctcttat attcaggaca
      781 aggtgctgat ttcataacct cttatttatt cttcatctgg tgtcattacc attggcaaaa
      841 tcattggatg tctttctgta cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt
      901 ctttcaattt atattctgaa cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata
      961 agattcgctg agcttcattg atcatttctc ctgattctcg catgtagaca aatccacgag
     1021 agagaatatc tggaccagcc aagatttctt ttttcttgat atccactgtc gcgacagcta
     1081 atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta ccaatatcac
     1141 cgatcccgct accatcgata tacacatctc cagccacaaa atgcccagca ggacgcgcag
     1201 aatcgctagt taacgctaaa acatcgccat tttccatgat gaagcagttt tctgcaggaa
     1261 cacctacatc ttgtgctaga gaagcatgta ctttcaacat gcggaattct ccgtggacag
     1321 gaataaaata ttttggcttc atcaaacgaa gcattaattt ttgttcttct tgtccaccgt
     1381 gaccggaagt atggatgtta ttgatttttc cgtgaatcac ttctgcacca gcttcagaca
     1441 gcaaattgat caaatgattg acacttgttg tattaccagg aataggtgaa cttgaaaaga
     1501 tcactgtatc gtctggctgg atttggattt gacggtgagt accattcgcg atgcggctca
     1561 acgcggccat cgcttctcct tgagaacctg tacataggat catcgtttca ttggcaggca
     1621 agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg aactttgata tatcctaaac
     1681 gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag
     1741 ttgccacagc ggcatctgct gcttgctgca aacggaagat gttagaagca aagctggcaa
     1801 aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat agaatgccca atcgtttttt
     1861 ctgatttagt aaatgttgga atctcagcat ttgtactatc agataaaagg cacaagactc
     1921 cttcttcccc gatacgagcc attttatgca agtttgctgg ttcgccaaca ggagtgaaat
     1981 cgaatttgaa gtctccagtc gctacgatat ttccagaagg tgttttcacc acgatcccta
     2041 atgcatctgg gatactatgg gtcgttctga aaaagctgac agaagtcttt ctaaaacgga
     2101 tgatcgtatc ttcattgatc tcatggagct ctgcatctcg aagcaagcca tgctcatcta
     2161 atttgtttgt gatcaacgct aatgcgagag gaccagcata gataggaata tttgcttgtt
     2221 tcagcaagta gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt
     2281 ttactttgtg taagttttgt atgatgtagc tgtagtcggg aatgacatag tcaattccaa
     2341 gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa aatgatttca tcttggaatt
     2401 gtaccccata cgtatttttc ccgatttcac ctaatccacc aatagcaaag acgcctgtct
     2461 cgttattttt gatggaaagt ttcatattaa aactccacta atgagaaatc agcattttct
     2521 ttttcgtact ctagatgact tccttctaga agttgaacga attcaatatt gtatggggta
     2581 ttttcttcca cacgttggcg agcgtctaca tcgctttttg cctcaatgta aagagatttt
     2641 gtttcttccc gtttagggtt gcgtactttt gtttcttgat aataaacttt atagatcatt
     2701 gttttgtctc ctttatctgc gtaaagcagt gatttagcta gttgattttt ttagacaaca
     2761 caaaacgagg ggacaagcca cctggttttt catatgcttt gactagaata ctggattcga
     2821 gtcaaagaga caagcctcgt ttttgcttat ctgttcggat attttttcaa ccgtaccttc
     2881 tgtataaaaa atatacaaca attaattgt
//
LOCUS       DAHUWM010000250         2894 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHUWM010000250 DAHUWM010000000
VERSION     DAHUWM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.256"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(61..1371)
                     /locus_tag="NK788_002622"
     CDS             complement(61..1371)
                     /locus_tag="NK788_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCH6770914.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="NK788_002623"
     CDS             complement(1383..2801)
                     /locus_tag="NK788_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6770915.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          902 a          603 c          451 g          938 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcat
//
LOCUS       DAHUWM010000251         2890 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHUWM010000251 DAHUWM010000000
VERSION     DAHUWM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.257"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..652)
                     /locus_tag="NK788_002624"
     CDS             complement(<1..652)
                     /locus_tag="NK788_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770916.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLL"
     gene            complement(639..1025)
                     /locus_tag="NK788_002625"
     CDS             complement(639..1025)
                     /locus_tag="NK788_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770917.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(1172..2842)
                     /locus_tag="NK788_002626"
     CDS             complement(1172..2842)
                     /locus_tag="NK788_002626"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCH6770918.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT          805 a          657 c          493 g          935 t
ORIGIN      
        1 cgagcagatt accatcggga ttcccatctt tagtgataag tacggcttcc aaagtccctt
       61 gctcacgaaa catcgcttca acctcgtaaa tatgtgtttt agctggaaca aaatgataat
      121 ttgtgtactt tccgttttgg atgatcaaat ccaacgctcg tttatttgtt aaatcaaccg
      181 gacctttttg gctttctttt gctagccaaa acccgatatt acgtaaagta atcaatgcta
      241 caaattttcc tttattgtat aacggaaatt gtgaatacct tttttctgct actatcttta
      301 acagttcaag taacggaaga tcttgttcaa aacctgttac acgttttgca aatcgaggca
      361 gcacggtttc aggctgaatc agttcttgtt cgatcagctg gattcgcttg actgtccaat
      421 catttggctc tgcaatgaca aaatcgactg cgatccgatc atggacgatc gcattcctta
      481 actgagcaag ctgtaaaaga tcatcttcgt attttctgat cattaattgt tttctttgag
      541 ctaggcgtct gacaagttcg gtaaatccca tatttcgtgc atttcccatc tcgtctctca
      601 tccatttttc gattcgattg aaactgatta aaaaaagatc agagtttgcc ataattttcc
      661 tccttgatca aatatgcccg gttttttata tagaaaaaga acgcaatcaa cacaataact
      721 gcaaaaaaac tgaaaaataa attttgtagg aaaacggccc ccaacccagt tgctagaaat
      781 aaaaacggat agatttgaag aatcaagtaa ttcattgtga tcacataagc aggctttgct
      841 tctttcactg tataaacggg gctttttagc caataaaagg tgacctgcat cgtaaagggc
      901 acttccagat caagcgttat cttttgttca tgactgagaa cactgcgttt ttcattgttg
      961 attcttagct gaatcgagct acccataccg taaaaaccgg tttttcttat aaatgtcgtt
     1021 ttcatcatac cctcctatta tgctttagta tagctcaaaa tacaataaag aaaaagaatg
     1081 atcctagtgt aaacaaaatt aattccctat cgtctaaaaa aagaagctgt agtcaaaata
     1141 cagctccttc gatggtagat tataatcaag cttaaaacaa tcccaacact tctccgtcat
     1201 ttgtcacgtc catattcaat gcagcaggtt ttttaggcaa accaggcatc gtcataacat
     1261 ctccagtcaa ggctactaaa aatcctgccc ctaattttgg gatgatttcg cggatcgtga
     1321 tggtgaaatc ttccggtgca cctagcagtg aaggatcatc agaaaatgaa tactgtgttt
     1381 ttgccataca aatcggcagt ttgtcccatc cattcttcgt aaactcttct atttgttttt
     1441 gcgctttttt gctgaaaaca acaccatttc ctccgtaaat tttcttgacg attgtttctg
     1501 tcttttcttg tatcgtttct tctaggcggt ataatggctt gtaattagct tcttttctat
     1561 caatcaatcg tactacagct tttgctaaat caacgccgcc ttctgctcca tctgcccata
     1621 cactcgctct cttggcaaga atcccctgat tttcacacaa gtgctccaac agtgtgagtt
     1681 cattgtctgt atctgttacg aattcattga ttgcgacgat cacaggtagc tgatattgtt
     1741 ccatattacg tatatggcgc tttaaatttg caaaacctac tttcaaagca tcaagattct
     1801 catttttaag ttcatctttt ttcattccgc catgcatttt caatgcacgg acagtggcga
     1861 caatcaccac agcatccggc gcttttttca agttaggtac tttgatatct aaaaatttct
     1921 ctccgccaag atctgcacca aatcctgcct ctgtgacaac gtaatctgct aatctcaatg
     1981 ctgtcttggt agctaaaata ctattgcagc catgagcgat attggcaaac ggtcccccgt
     2041 ggacaaaagc tggtgttccg taaattgttt gtacaagatt cggcttgatt gcatctttca
     2101 gcagtaatgc cagagcgcct tctgctttca agtctccagc agtcacaggc tgacggtcaa
     2161 atgtatagcc gacaacgatc cttgcaagac gagcctttaa atcttcaagg tctgccgcaa
     2221 gacacaagat agccatgatt tcgctggcaa ctgtgatatc aaacccatct tctcttggaa
     2281 caccttggat tggcccaccc aagccaacaa ttactttacg cagttcacga tcattgagat
     2341 cgactacccg tttccaaatc actcggcggg catcgatgtt caattcattt ccttgatgaa
     2401 tatgattatc taaaagagca gataatgcat tattagctgt tgtgatagca tgcatgtcac
     2461 cagtaaaatg caagttgata tcttccatag gcaatacctg tgcatatcct ccgcctgcag
     2521 caccaccttt gattcccatc acaggaccaa gtgagggttc tcgcaatgca ataacagatt
     2581 ttttattgat tttattgagc gcgtctccta accctacagt gatcgttgac tttccttcgc
     2641 cagctggtgt tggattgatg gaagtaacta agatcaaatg cccctcagcg ttatcctttg
     2701 atcgattgat tccagaaaaa ttgattttcg ccttatattt cccatacagt tccaaatcgt
     2761 cttccttaag atctaattta ctagccactt caacaatcgg ttctagctct gtttcttgtg
     2821 aaatttgcag atcattcttc atttgattac cccttttcac gaactttatt caacaaaata
     2881 tcttctttgt
//
LOCUS       DAHUWM010000252         2825 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHUWM010000252 DAHUWM010000000
VERSION     DAHUWM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.258"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            105..2714
                     /gene="clpB"
                     /locus_tag="NK788_002627"
     CDS             105..2714
                     /gene="clpB"
                     /locus_tag="NK788_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCH6770919.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         1000 a          487 c          627 g          711 t
ORIGIN      
        1 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
       61 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
      121 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
      181 atcaagatat cgacattgcc catgtatgga aaatcttttt acaacctaat cattttggtc
      241 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
      301 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
      361 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
      421 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
      481 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
      541 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
      601 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
      661 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
      721 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
      781 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
      841 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
      901 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
      961 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
     1021 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
     1081 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
     1141 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
     1201 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
     1261 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
     1321 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
     1381 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
     1441 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
     1501 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
     1561 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
     1621 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
     1681 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
     1741 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
     1801 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
     1861 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
     1921 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
     1981 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
     2041 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
     2101 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
     2161 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
     2221 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
     2281 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
     2341 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
     2401 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
     2461 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
     2521 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
     2581 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
     2641 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
     2701 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
     2761 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
     2821 ccgaa
//
LOCUS       DAHUWM010000253         2733 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHUWM010000253 DAHUWM010000000
VERSION     DAHUWM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.259"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="NK788_002628"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="NK788_002628"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCH6770920.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1069 a          424 c          558 g          682 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atc
//
LOCUS       DAHUWM010000254         2649 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHUWM010000254 DAHUWM010000000
VERSION     DAHUWM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.260"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            256..531
                     /locus_tag="NK788_002629"
     CDS             256..531
                     /locus_tag="NK788_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770921.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="NK788_002630"
     CDS             544..2616
                     /locus_tag="NK788_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCH6770922.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
BASE COUNT          811 a          413 c          569 g          856 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaa
//
LOCUS       DAHUWM010000255         2638 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHUWM010000255 DAHUWM010000000
VERSION     DAHUWM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.261"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(562..1065)
                     /locus_tag="NK788_002631"
     CDS             complement(562..1065)
                     /locus_tag="NK788_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCH6770923.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1424..2449
                     /locus_tag="NK788_002632"
     CDS             1424..2449
                     /locus_tag="NK788_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCH6770924.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          857 a          408 c          485 g          888 t
ORIGIN      
        1 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
       61 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
      121 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
      181 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
      241 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
      301 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
      361 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
      421 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
      481 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
      541 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
      601 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
      661 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
      721 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
      781 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
      841 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
      901 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
      961 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     1021 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     1081 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     1141 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     1201 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     1261 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     1321 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     1381 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     1441 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     1501 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     1561 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     1621 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     1681 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     1741 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     1801 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     1861 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     1921 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     1981 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     2041 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
     2101 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
     2161 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
     2221 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
     2281 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
     2341 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
     2401 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
     2461 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
     2521 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
     2581 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaacttt
//
LOCUS       DAHUWM010000256         2621 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHUWM010000256 DAHUWM010000000
VERSION     DAHUWM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.262"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            416..622
                     /locus_tag="NK788_002633"
     CDS             416..622
                     /locus_tag="NK788_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCH6770925.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            622..873
                     /locus_tag="NK788_002634"
     CDS             622..873
                     /locus_tag="NK788_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770926.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            888..1325
                     /locus_tag="NK788_002635"
     CDS             888..1325
                     /locus_tag="NK788_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770927.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1318..2025
                     /locus_tag="NK788_002636"
     CDS             1318..2025
                     /locus_tag="NK788_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770928.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2406..2585
                     /locus_tag="NK788_002637"
     CDS             2406..2585
                     /locus_tag="NK788_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770929.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          947 a          443 c          547 g          684 t
ORIGIN      
        1 tgaggattgc agctcttttt gttttacata gaaacaggaa aagacctttt taggaagaca
       61 caagtgcctt ctcaattgaa aaacctttct ctcactccaa ccgccaccgc cgttcgagaa
      121 gaaaggaagt gaacgttgca cgttcactaa gatatggtgt cttgcaaagc aagacaaaat
      181 agaaaaaact tcctctcacc aaaaggttcg ttcctgttcg ctatctcttt attgatttgc
      241 cagaccggca acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca
      301 cgctgcgcgc ccttgtcctt gtcgcttttc gttgattcat tctggccaac aaatcaataa
      361 agaaagaact aacgaaagga aataaaacct ttcctaagag gaggcagaat caatcatgcg
      421 aaagaaattt gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc
      481 acatactgtc gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct atcgtttgta
      541 tcaatgtcca acatgcggga aaaaatatac cttgtcttat ggcgattatg ttttgattga
      601 tgagaaagta aagaaacggt gatgaaaaca caacacaaat tattgactgt aaaagaagtc
      661 gttcaaattt tggtggacaa agaagtcatt tctgaaaaag tagcagaagc tttacttatt
      721 ccgcggtact ggacgataga aaccccttta gtagaggatg caccgactat gctatttgaa
      781 gacctagtga agattgcagg atatgaactg cattatcaag aaaacatgga aacttatgaa
      841 atcgaatatt ggttaaccaa attagagaac taaaaaggag aaaaaatatg gctcaaaatt
      901 actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg gaggatatga
      961 cgttcctata ccatgaaaca cgtgtaccca aaaaacatta caaggaaaaa ttgtcagtcg
     1021 cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg tactatcgaa
     1081 cactggaaga actgaaaaaa caaaatggaa aatggttttt ccaagcctta ctatgcttgg
     1141 aagtaggtgt caaaccaagc actatcaaac cttccgaata tcaagcctta gaactgactt
     1201 atgcaaaatt cgcacagatt aagaaagcta aagcaatggc tagcgactgg ctagatactt
     1261 ttgaaaatat cgaaaaaaat ggtgcatttt atacgattga atcggaggag aaagataatg
     1321 aataaggtgg tttatcagct aacaacagaa gatgtttcgc aatttttaac aaacaaagaa
     1381 cacttgaatg aagtaatcag acggattcaa aaagacttta gttgtatgga agaaatgaag
     1441 tactttattc aaagagtgga agaacagttg tatatggaaa gtgaaacgag tcaaacgaag
     1501 gttacagtca gccataatcg tttagacgtg tataaacgtc gtcctgacac attaaaaaaa
     1561 ggagcggagc ttctacgact agaaacagaa aaatatatca tgaatgttta cctagaaagg
     1621 agtgagctgg tcgaaaactt tgtcagctac acgatttgca ggaaacacta ttactcagaa
     1681 tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat ggaagagcta
     1741 aacagcaaag atgcgacggt agacgattat taccttgtat ttactagatt tttgacgtgt
     1801 gggacggaat ctatcgtgtt aatgggaatg gattatgaac tcactgtacc cgaagcattt
     1861 ttgagggaat atgtagaaga agtagataac ccaggcgacg actggtacac gactttaact
     1921 gattttttag acacctacac ttacgacgat acagaagaaa tccaaaatga acttgaccgt
     1981 aaacgaatgc catatcaacg atttgaaagg aaaaaaggtc aataaccatt tataccgaaa
     2041 agtgaggatg tgtgaaataa gaacaaaaaa ccttgaaatt aagggaatgg ttcttatttc
     2101 gcaccaatcg atccaaccgc ccccgccgct taggtggtgc gaaataagcg ggcaaagccc
     2161 gataacctaa gagaaagaag gagatccata tgaataaaat cattcctcta ttgttcgtac
     2221 tgtttgttgg cgagatactt atgcgtcatg gtgcagctct acatttaaac ttcgtagtat
     2281 tcttaggaat actggtattt tgtttgatct tttggcgctt tgtgttaacc cttgtgagtc
     2341 taatattcaa gttgattgca gccatatttt taatagggtt atttcgatga aaggagagag
     2401 tactaatgcc aattgcaata ggaaataaac ggttacccgt gacgttggat gaaaagagac
     2461 aaaaagaatt gcagcaacta aagcagaagt acggcaaaag tgaatccagg attatgtgta
     2521 ttgcgttaga tttattgatt gcccaagaaa aagcaggatt tgaggtacca gcactcaaaa
     2581 agtgacgtca ctttttatcc taaaaactaa aagtgatagc a
//
LOCUS       DAHUWM010000257         2617 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHUWM010000257 DAHUWM010000000
VERSION     DAHUWM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.263"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(218..1270)
                     /locus_tag="NK788_002638"
     CDS             complement(218..1270)
                     /locus_tag="NK788_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6770930.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1283..2221)
                     /locus_tag="NK788_002639"
     CDS             complement(1283..2221)
                     /locus_tag="NK788_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCH6770931.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          854 a          559 c          398 g          806 t
ORIGIN      
        1 ttttttctca ttttaacaca tatttttatt ctagttgtct tttgaacaat caataacata
       61 aaggtgagtt tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc
      121 atcaatctcc tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt
      181 acgcatccat tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt
      241 cttctcgatc atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa
      301 aaacagggat gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag
      361 aagccaacgg accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa
      421 tagaaaatag agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa
      481 tcacaggttt tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa
      541 ttgtcacacc tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag
      601 ctacgcccat cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg
      661 ctactgttga aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca
      721 ttaacagcgg ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg
      781 ctgttacaaa agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg
      841 gtgttaacac aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag
      901 caatcgatgc tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac
      961 ccgtttcacc caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg
     1021 ctacaaccat cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga
     1081 tcaaaggtgt tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat
     1141 acgcactaaa atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga
     1201 ttcctgttgc tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg
     1261 aagtttccat tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa
     1321 gctcagtcaa gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa
     1381 ttgcgccatt gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca
     1441 ttgaaggata atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga
     1501 cttctgggac atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa
     1561 tcgtcgccac cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta
     1621 agattgtgca tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt
     1681 gaatattgtc agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt
     1741 ttgcagctgc ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata
     1801 atttgacctg accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat
     1861 tttccattgg tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata
     1921 agacctctgt atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca
     1981 tcgctttggt aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa
     2041 gaacgattgg tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgtt
     2101 ctttgatttg ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt
     2161 ggtgaagcat caacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca
     2221 ttgattcttc ctcctttttc tttctattat actcatttga gaatcttttc acaacctcgt
     2281 tttgttattc ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca
     2341 aatcaataaa aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata
     2401 agaaaataga aacattcata gaaaaaatct cactcctctg attataaaag agggggcatt
     2461 aacctcgtgt atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa
     2521 atattcattg tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta
     2581 aaatcgaaca atacacttat ttaaaaactt ctttccc
//
LOCUS       DAHUWM010000258         2564 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHUWM010000258 DAHUWM010000000
VERSION     DAHUWM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.264"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            13..1047
                     /gene="sppA"
                     /locus_tag="NK788_002640"
     CDS             13..1047
                     /gene="sppA"
                     /locus_tag="NK788_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCH6770932.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            1049..1570
                     /locus_tag="NK788_002641"
     CDS             1049..1570
                     /locus_tag="NK788_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCH6770933.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            1567..1953
                     /locus_tag="NK788_002642"
     CDS             1567..1953
                     /locus_tag="NK788_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCH6770934.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT          878 a          336 c          497 g          853 t
ORIGIN      
        1 ggaggagttt atatgaataa aagaagatgg attgctgtgg gagtagcgtg cggattattg
       61 ttactatcca ttatcgtcgc gctaattcca ggaaaagata aagaggaagc atcaaataca
      121 accctaacag gaataaataa gattttttac ggatctaacg aaatcacaga agaaacgtta
      181 gaagaaggcg catcaaataa aaagattgtc aaactatccg tcaatggagt tattgcagat
      241 acaggtgaaa gcaatctttt tagtagggaa caatataacc atcagaattt tttaacacag
      301 ctaaagaaaa ttcaagaaga caaagcggtt aagggagtgt tattagaagt aaattcacct
      361 gggggaggaa tatatgaaag tgctgagatt gctaaagaaa tggcaaaaat taaaaagcta
      421 gatattccta tctatactgc atttaaaaat acagcggcaa gtggagggta ctacatttct
      481 gctggttcag ataaaatatt tgctacggaa gaaacaacaa ctggatcaat tggtgtcatt
      541 atttctggtt taaattattc tggcttatta gaaaaacttg gcgtaactga tgcgacgtat
      601 aaaagtggtg cattaaaaga tatgatgtca cctcaacaca aaccttcaga ggaagagaat
      661 aaagttatac aggaatttgt gatgagtgct tatgatcgtt ttgtcaatgt tgtagcaaaa
      721 ggaagaaata tggatactaa tgcagtgaaa gaacttgcgg atggaagaat ttatgatggt
      781 aaccaagcag tagaaaatgg gttggtggat caaattggtt actctgaaga tgcattagat
      841 tccctaaaaa aagagaagaa attaactgat gcgacggtta tagaatataa aaatgacaca
      901 actggctttg cttcttcttg gttaggtaat aagattgctg aatggcaagg gctaaaggca
      961 actacatcag atcgtgtaat ttcagttttt gaaaaacttg gattttctga tactccaaaa
     1021 ccaatgtatt actatggagg gctataaaat gaataagaag agtggcattg aaactttgcc
     1081 taattatgta tttgcgggat tttggttacg attttttgct tttcttattg atttaatttg
     1141 tattagcatc atcacccatg ctactatagg tctcttttac aaaatcatgg gactggaatc
     1201 tgacaaatcc attattactt tttatggtat agcgtcatta gctatttatt taacctattt
     1261 tgtacttttg actaagctga attcaggaca aacaattggg aaaatgattt ttgggataag
     1321 agtagtgagc tttgaggaaa aagagctaag ttggcaaact atctttattc gtgagtgtat
     1381 atgtaggttt atattaaaaa attttgtatt tcaaattggg tatttggtgg ctgctttcac
     1441 gcccaagaaa caacatattg gggattattt ttctaacacg tcagtggttg ttattcgttc
     1501 attagaagct attaactatg gtcaaaaaat ttctaattct aaaacactta atctggagag
     1561 aattaaatga ataagagaaa aacagagttt attttgattt taattagtgg aataacaggg
     1621 attttatgta gtcttttagc attgatgggt ggtatgtttc tatcttctat tgattcaact
     1681 actttaaata ctacagaact aaccagtaag gatatagata tgttagtcaa tctaagcggg
     1741 aatgttgttt tatttggagg aattacccta gttttttcga taatgatttt cataataggt
     1801 tttttagtaa gaaaaaatca aaaaacagtg gtctatggta ttagtttact cattttatct
     1861 attattccat tttttctttt aacattattg tgggtaattc ctggagtttt aggtattatt
     1921 gcgggattga tgcttctaat aagaaaatct taattaggca atgtgtagac aacgtgaaac
     1981 gattaatcaa ttaaaagcta aatacgtttt gagaaatgat aacttgtagt cgatggaaga
     2041 catgtagata aaaaaactac gtgtctttta tttattctaa ttttttcgaa aaaaagacac
     2101 tctctgattg ttgtattaat ggaaaagggg aaagcagcaa gtaaatttag tttttctatt
     2161 aaatattctt cactttttaa ccttttatag tacttagatt taatcctata tgtttagaga
     2221 gttcattggc agcatgattt tgcaaagctt ctcgaattat tgttatatta gaaactttac
     2281 tgttcataat tatgcttctt ttttaactca aagtccaaag ggctacagtg gactttgagt
     2341 taaaaataaa aagccctagt tattttgaaa aataaaaaaa gaacagcgta aatgttttcg
     2401 aaatgctttc gcaccaacat ttacctgttc cgtgggtttg gcacccaatg ggccgtgagg
     2461 ggctcgaacc cgcgacccgc tgattaagag tcattaatgt caatttttaa ataaaattaa
     2521 tatgtctctt taagtattaa actatcactc tttatcctat gata
//
LOCUS       DAHUWM010000259         2563 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHUWM010000259 DAHUWM010000000
VERSION     DAHUWM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.265"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            1..1452
                     /locus_tag="NK788_002643"
     CDS             1..1452
                     /locus_tag="NK788_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCH6770935.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="NK788_002644"
     CDS             1801..2433
                     /locus_tag="NK788_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCH6770936.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          954 a          374 c          506 g          729 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gta
//
LOCUS       DAHUWM010000260         2556 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHUWM010000260 DAHUWM010000000
VERSION     DAHUWM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.266"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            3..1010
                     /locus_tag="NK788_002645"
     CDS             3..1010
                     /locus_tag="NK788_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6770937.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1022..2026
                     /gene="neuB"
                     /locus_tag="NK788_002646"
     CDS             1022..2026
                     /gene="neuB"
                     /locus_tag="NK788_002646"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCH6770938.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2023..>2556
                     /locus_tag="NK788_002647"
     CDS             2023..>2556
                     /locus_tag="NK788_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HCH6770939.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDT"
BASE COUNT          933 a          332 c          512 g          779 t
ORIGIN      
        1 aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt ggaagtgaaa
       61 ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt agaggtgaag
      121 aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga tttttaattg
      181 gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat attatttttc
      241 atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa
      301 caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat gtaaaacatt
      361 ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt gcgacaaagt
      421 tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca gaaacaatat
      481 tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt cctcttttta
      541 gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg acacgattta
      601 tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat tcaaaatttg
      661 gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct caatcattaa
      721 taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa gttgtaggta
      781 tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt gaacgctcat
      841 atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac gaactgtaca
      901 aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa ttaatgagtg
      961 gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa ggagttttat
     1021 gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg gaagtctaga
     1081 tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg ttaaatttca
     1141 aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat atcagaagga
     1201 taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat tatctcaatc
     1261 tgattttatt agaataaaag attattgtat acaaaataaa attgattttt tgtctactcc
     1321 ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat ttaaaattgg
     1381 ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg ggttgccagt
     1441 tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag attgtttcat
     1501 agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt ctgaagatat
     1561 taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg ggtactccga
     1621 tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta aggtaattga
     1681 gaaacatttt accttggata aggaactacc tggtccagat cataaagctt ctttagatcc
     1741 tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt taggtgatgg
     1801 tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa aaagcctagt
     1861 ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta caataaaacg
     1921 tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa aagttaaaag
     1981 accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa aatacttata
     2041 gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa tttactacaa
     2101 gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa atatggattt
     2161 actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca aagtttgttt
     2221 ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa tggtttcaca
     2281 aatatatttg aacaaactag ccctgatttt gtattattac taggggatag aggagaaatg
     2341 ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat tcacggaggc
     2401 gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat agcatctgtt
     2461 cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga agattcttgg
     2521 agagtttttc ccgtcggagc tcctagaata gatact
//
LOCUS       DAHUWM010000261         2539 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHUWM010000261 DAHUWM010000000
VERSION     DAHUWM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.267"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            463..1482
                     /locus_tag="NK788_002648"
                     /pseudo
     CDS             463..1482
                     /locus_tag="NK788_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1499..1648
                     /locus_tag="NK788_002649"
     CDS             1499..1648
                     /locus_tag="NK788_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770940.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1645..2091
                     /locus_tag="NK788_002650"
     CDS             1645..2091
                     /locus_tag="NK788_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770941.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2093..2230
                     /locus_tag="NK788_002651"
     CDS             2093..2230
                     /locus_tag="NK788_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCH6770942.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2268..>2539
                     /locus_tag="NK788_002652"
     CDS             2268..>2539
                     /locus_tag="NK788_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770943.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          925 a          435 c          520 g          659 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taatctactt
      481 gatgcaccgt attatttgag taaggtggct ttgggtgaga atctaattaa accagaatca
      541 caacaaccag ttactaatag taactatctt attaacacct ataacattaa accaatggta
      601 aaaggtaaga agtataccat cacacttgaa ggaactaagc cagcaacaca ggtttttaga
      661 ccatttttca cacgagcaac aggagatgca tgggaggttg gtgacttaca accagtagaa
      721 gggttaactg atgtttggtc tggaacattt acagcagcag ctgacccaca ccctaccagc
      781 ccacaagttc agatttgtca ggtaccaagc acaagtttag gaaactgtac aattaaatgg
      841 ttaaaactag aggaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga
      901 atcggcatgc gagactcaaa caatccaaaa gattacgtct gggatctagc accagaatat
      961 gtcgaagaca atctggccac agatattaaa atttctgaaa ttactggtaa agcaaacaat
     1021 tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat taatgaagta
     1081 gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa
     1141 ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt aacagttaca
     1201 aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt
     1261 gtggctttaa acggaagaag cagtggtgga aatgattcaa cgatactaac aattccagaa
     1321 ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg
     1381 aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt
     1441 tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt gaatcgatat
     1501 gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga
     1561 agaacattta ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa
     1621 attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg tactggcgga
     1681 gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca
     1741 ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat
     1801 cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga tttaaacaat
     1861 ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca gatcaatgcc
     1921 aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa agaaaaagcg
     1981 gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac
     2041 atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag
     2101 ttttagcgat gtgaaaatga tgtatgattg gggctgtttc actaacgaac aagtaatggt
     2161 tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa atcattagca aagaagagag
     2221 cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag
     2281 gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     2341 taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca
     2401 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
     2461 aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg gtagaacaca
     2521 cgcaacaaat taaaacttt
//
LOCUS       DAHUWM010000262         2521 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHUWM010000262 DAHUWM010000000
VERSION     DAHUWM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.268"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            4..2376
                     /locus_tag="NK788_002653"
     CDS             4..2376
                     /locus_tag="NK788_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770944.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1010 a          398 c          554 g          559 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcatatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 g
//
LOCUS       DAHUWM010000263         2520 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHUWM010000263 DAHUWM010000000
VERSION     DAHUWM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.269"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            10..261
                     /locus_tag="NK788_002654"
     CDS             10..261
                     /locus_tag="NK788_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCH6770945.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            363..2360
                     /gene="tkt"
                     /locus_tag="NK788_002655"
     CDS             363..2360
                     /gene="tkt"
                     /locus_tag="NK788_002655"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCH6770946.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          837 a          466 c          570 g          647 t
ORIGIN      
        1 gggctgagca tgctgtcacc tgaaaaaata gagcggatca atgaacttgc acgtaaaaag
       61 aaggaaaatg gtctctcaaa agccgaagaa caagaacaaa tcaacttgcg caaagagtat
      121 ttggaagctt ttcgttccgg tatgcgtcat catatcgaag gaatgaaagt cgtcgatcct
      181 gagggcaatg acgtgacccc ggaaaaatta aaagaaatcc aaaagaaaaa aggcttacac
      241 aaccgaaatg acaactttta gtaaaaaata ctgaaaaaga ttgctttttc gtgtgacatc
      301 ttatagaatg aaagtgtaag aaataaaggt aaaaaaaacc taaattaata ggaggggtta
      361 ttttgtttga caaaattgac caacttggcg ttaacacaat tcgtacatta agtattgaag
      421 cagtccaaaa agcaaactca ggacatcctg gattgccgat gggcgcagca cctatggcat
      481 atgcgttatg gacaaaacac ctgaaagtaa atccaacgac atcaaaaaac tgggctgacc
      541 gcgaccgttt cgttctttcg gcaggacatg ggtcagcaat gttgtatagc ttgcttcatt
      601 tagctggcta tcaagtgaca atcgatgatt tgaaacaatt ccgtcaatgg gattctaaaa
      661 caccaggaca tcctgaagta catcatacag atggtgtaga agccactaca gggccattag
      721 gacaagggat tgcaatggct gtcggtatgg caatggcaga agcgcacctt gcagcaacgt
      781 ataacaaaga aaactttaat gtgatggatc actatacgta tgcaatatgc ggtgacggag
      841 acttgatgga aggggtatct caagaagcaa gttcgatggc aggtcatatg aaactaggga
      901 aattgatcgt tttatacgat tcaaacgata tttcactaga cggtccaact tcaaaagctt
      961 tcacagaaaa cgttggtgct cgctatgaag catatggctg gcaacatatc ttagtgaaag
     1021 acggaaatga cctagaagca atctctaaag cgattgaaga agcaaaagct gaaacagaca
     1081 aaccaacatt gatcgaagta aaaacagtca tcggctatgg tgctcctaaa gaaggaacat
     1141 ctgctgttca tggtgctcct cttggtgaag atggcatcaa gatggctaaa gaagtttatg
     1201 gttgggagta tccagatttc actgttccag aagaagtagc tgcccgcttc catcaaacga
     1261 tgatagaaga aggacaaaaa gctgaagatg catggaatga aatgtttgcc aattataaaa
     1321 aagcttatcc tgaattagca caacaatttg aagatgcttt tgatggcaaa ctaccagaaa
     1381 actgggatgc agaattgcca acttatgaag ttggaagcag tcaagcaagc cgtgtttcaa
     1441 gtaaagaagt catccaagaa ttatcgaaag ctataccaag cttctggggt ggttcagcgg
     1501 acttgtcagg ttccaataat acaatggtgg ctgcagataa agatttcact cctgaacatt
     1561 atgaaggacg caacatctgg ttcggtgtcc gcgaatttgc aatggcttcg gctatgaatg
     1621 gtatccaact tcatggcgga actcgtatct acggtggaac attctttgtc tttgtcgatt
     1681 atctaagacc agcagttcgt ttagcagcaa tccaacatac tcctgttgtt tacgtcttaa
     1741 cacatgactc ggttgctgtc ggagaagacg gaccaacgca cgaaccgatt gaacaactag
     1801 catctgttcg ctgcatgcct ggtgttcaag tgattcgtcc ggcagacgga aatgaaacac
     1861 gtgctgcttg gaaagtagcg atggagacaa cagatgcgcc tactatcctt gtattgagtc
     1921 gtcaaaacct tccagtattg ccttctacaa aagaagtagc agatgaaatg gtgaaaaaag
     1981 gcgcgtatgt tctttctcca tcacaaggag aaacaccaga aggtattttg attgcaacag
     2041 gttctgaagt tgatttggct gtgaaagctc aaaaagaatt ggctgaaaaa ggcaaagatg
     2101 tttctgttgt ttctatgcca agctttgacc tctttgaaaa acaatcttct gaatataaag
     2161 aaagtgtact tcctaaatca gtgaagaaac gtgtggctat cgaagctgct gcatcatttg
     2221 gatgggaacg ctacgtagga acagagggcg caacgattac aatcgatcat ttcggtgcat
     2281 ctgcccctgg cactaaaatt ttagaagaat ttggatttac agtagaaaat gtcgtgaata
     2341 cgtacaatca actatcatag atccaagcaa ttgtttgact gataagaaca gctgtctgca
     2401 agttggagac agctgttctt tatgtgttag aagaataaat caatattact tttcgttttc
     2461 ggctatgtta catttaaatg acaaaacaaa gaattcttcg gattagtttg tgtttcttga
//
LOCUS       DAHUWM010000264         2510 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHUWM010000264 DAHUWM010000000
VERSION     DAHUWM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.270"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            285..518
                     /locus_tag="NK788_002656"
     CDS             285..518
                     /locus_tag="NK788_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCH6770947.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            515..922
                     /locus_tag="NK788_002657"
     CDS             515..922
                     /locus_tag="NK788_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCH6770948.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(2059..>2510)
                     /locus_tag="NK788_002658"
     CDS             complement(2059..>2510)
                     /locus_tag="NK788_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCH6770949.1"
                     /translation="EFETIPEELKDKRVEEVDRTPAENLAYQVGWTNLILKWENDERN
                     GLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNIDDINMMIDSMSDEDL
                     FTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRLAL"
BASE COUNT          864 a          417 c          390 g          839 t
ORIGIN      
        1 actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat taatttgttt
       61 caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt atacataaat
      121 agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt aattatatag
      181 aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt taaaatcaac
      241 aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca attaaagaaa
      301 gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag tttcttaaag
      361 aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt cgagcggcaa
      421 ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa gcattaactg
      481 aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc tagaagccaa
      541 agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg aaccccttgg
      601 tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct cagcttttgg
      661 tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac aattgataaa
      721 gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt ttgtcttttt
      781 gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt tagcggtata
      841 tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca acactataaa
      901 gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt tattagctgc
      961 tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa ctatatagaa
     1021 aacgtaaatt ttctatatag ttggattaag ataacgacta tttattttat catattttta
     1081 ctatacccta tacttttact tggtatattt atgaataatc gcaaatttac ccctatacta
     1141 ctagtttgta agttagatta ataaaaatac taaagttatt tgttctttta ttaaatacac
     1201 ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata acagctgata
     1261 gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt
     1321 ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcggtac tgggattaaa
     1381 ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt gatttattta
     1441 ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa tttatttaag
     1501 atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga tggttagaat
     1561 aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg ctatctccaa
     1621 attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta acccaaagtc
     1681 agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga ttccatattt
     1741 aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt
     1801 cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct cattttatcg
     1861 aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac gatgcaccct
     1921 ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca gttagcaaag
     1981 aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt ccacaattgc
     2041 ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct ttgttctaaa
     2101 agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc atacagccgt
     2161 tttggttgcc tcgtccgccc attttctttt gtgtgcagtg aatagatctt catcactcat
     2221 tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct ctaattcttt
     2281 caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta actgattcca
     2341 tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat tttcccattt
     2401 tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg ttctatccac
     2461 ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta
//
LOCUS       DAHUWM010000265         2482 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHUWM010000265 DAHUWM010000000
VERSION     DAHUWM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.271"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(69..683)
                     /locus_tag="NK788_002659"
     CDS             complement(69..683)
                     /locus_tag="NK788_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770950.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            921..1343
                     /locus_tag="NK788_002660"
     CDS             921..1343
                     /locus_tag="NK788_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770951.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1353..1556
                     /locus_tag="NK788_002661"
     CDS             1353..1556
                     /locus_tag="NK788_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770952.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1767..2351
                     /locus_tag="NK788_002662"
     CDS             1767..2351
                     /locus_tag="NK788_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770953.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          819 a          386 c          443 g          834 t
ORIGIN      
        1 taattttata gatagtaatt atatgtagga aattaaagat gagaagataa taaaagcagc
       61 atgatcaact atttgtcatt gaccatgctg tcttttttta ttctatacac ggtttgtctc
      121 gtaacattgt agtctttagc aatcttagca atcgcaacac catttttaag atcttcaacg
      181 atgcttttgt aaacaaggcg acgttgggga tcttttgcgt ctgcactgta taatttgggc
      241 cgccctttgt agacgccgtt tgcttttgcg atcttaattc cttgtgcctg gcgacgtttc
      301 gattcagtcc gttcttgttc agcgatcatg gccaaaattt ggatgatcag atccttaatg
      361 aatttatcca ggagaggatt gccaattgct tcagccatga tcggtaaact agtaatgatc
      421 agcttcacat ttttcttctt caagtaattg actgaagcga tgatctcgtc ataatttctt
      481 ccaagtcgat caatagcttc cacaataaaa atatcttgat ctcggacgta atctaatgct
      541 tcttggaaaa caggtcgatg agtaatcgtt gcgccagatt gtttctcgat gaaaattctc
      601 tctgcaccga attttttcat ttcttcaatc tggcgttgtt cattttgatc gatcgatgag
      661 acacgtatgt atgcaactct caaaactttt acctactttc tgcaactatt taaaatacac
      721 cctaatcata cacgaggaag ccatatgtca cttttctttt taaatggcaa tattttttgt
      781 aaataaatgt caatcaagaa aatttaaacg attatgacat taaaaatata ttttatgaat
      841 tttctagtat ataacttgat ttatctgtta agatttatta agaaaacgtt ttcttagttt
      901 tgatttcgag gtgatagaca atgaaaaata agtttatttt atctttaggt attttaacta
      961 ttggaattag tgtaactgga actacaattc ctacatttgc tgatagtaca aatgaaccaa
     1021 ctactgtagt agataataac caagaattaa ctcctttaga tagggacgga atagccattg
     1081 gtaacccaac taacgactta actgttactg aaaattattt aatagtttct ccattacttg
     1141 ctctagcagc ttctaaaacg atacgagtga caggaacgat gatttttgat aaagttggtt
     1201 ggccttctag acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt tattggtact
     1261 atggaaaatt gtatttacaa agttataaat cagctggaaa caattggatt gcgacttatt
     1321 caggaacctt aactagaaag tagggatttt ttatgccaga atttgtgaac acaccaaatt
     1381 tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa tcagctttac
     1441 aagttcagcg aactttacag gagacacaaa aaattcaaaa atcaatttct aatctagaag
     1501 attctgaaga aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa aaataatgat
     1561 tttaaaaaaa gagtgtctag tcgacattct ttttttctaa ttgaaagtgt tcataaaatc
     1621 acccatttct ggacactttc tttttttgct ttatttttca aaaaaacaat tcatttcatg
     1681 ttcataaacc cataacaata tctatgttca gttaaactat atggataaag ttatgttaca
     1741 ataaaattaa tgaaggagtg atttttttga aaataggata cgcccgggta tctactggtt
     1801 tacaaaattt ggatttacaa gaagacagcc taaataaatt tggttgtgaa aaaattttta
     1861 ctgatcatat gagtggggca aaaagtaatc gaccaggatt agaaatggcc attgattttg
     1921 ttcgctctgg cgatacactt gtcgtttggc gcttagatcg tttaggtaga aatatggaag
     1981 atttgatttc aatcgtcaat cgactgaatg agcgtggtgt gagttttcat agtattcaag
     2041 aaaatattac gatggacaaa tcaagttcta caggacagct catgtttcat ttgtttgcgg
     2101 catttgcaga atttgagcgt aatttgatcc tggaacgatc cgcagcagga cgagcagctg
     2161 ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa gatttggaat
     2221 tactgaagac tttagtggat agcggtacac ctattaagac tattgcggaa cggtggaacg
     2281 tctcaagaac caccatttat cgttatttgg caaaaatgga ggagaaaaat ggaaagacaa
     2341 attctcatta gtttttgtca tttaatgctg aaaatttttg ccatttatct agaaaagtga
     2401 caatagtatg agaatttttt gtaagtgtct actgataact tattctcaaa tgttgtaagg
     2461 attttattca tttgttaata tt
//
LOCUS       DAHUWM010000266         2463 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHUWM010000266 DAHUWM010000000
VERSION     DAHUWM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.272"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(379..2014)
                     /locus_tag="NK788_002663"
                     /pseudo
     CDS             complement(379..2014)
                     /locus_tag="NK788_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          838 a          433 c          368 g          824 t
ORIGIN      
        1 gaagcatgta attttttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca
       61 tcacttactt gtgtaaaata gcgacgttta atgacaaaga tatcttcatt gctatctagt
      121 gtaccttcat ttctttgtgc ttaaattcaa agataatttt ctcaataaag atttttgatt
      181 ccagtaagat tcctttactc cttttacaac atagcgttgt cctttgtttt gaaacatctt
      241 cttcccctct ttgccattaa aaatagccca attctctata aagaagatta agcttcatta
      301 ttataatata aagtttaaat ttggtttatt acttactttt tcttattctc tagtaagcta
      361 atattgaaaa aaatgtaatc agatcattat ttttatatgt tgtaacttcc tgataagaaa
      421 actaattaaa agaaagaggt aactacataa actaattaaa aaagctaact tcatagttgg
      481 aggataaatg gaagaaactt caatgattgt ttctccttgt gttccttcaa tcgacactag
      541 tcctattatc aatctctctt ttttgtccat ttacttttgc gactgttccc caataatcaa
      601 atacaggaag cgttattttt tgtcctttag attcatgtac tgtatagact attttcgaat
      661 ccatcacttg ataaggcata gtatgccatt ccttgccaat tcttacttca tgattctgca
      721 aatgttcttt gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga
      781 gtgtcgtgta acgaataaga ttttcttgat tcattaccaa gtgatctctc tgagtgacaa
      841 ctttatgttc atagtttgct tttgttgcag cagatacagc caacagtaat aaaacaattg
      901 ccgttatatt aactactttt ctttgcttag ctgatgtgct cttcatgaaa acgaacgata
      961 aaatcaacga gccaaacagt acttgaaagc ctaagattcg ccaaggaaat tgaatcattt
     1021 gaatgggtgt attttgtaat aaagaccatg gaaaaaattt ggtggataag agccaagaga
     1081 ctaccgcact aaggtaaatc actttttctt tgcgattaaa tcctttaaaa aagaagagtg
     1141 gacagatcaa agaaataata ccaatgattc ctaccgtaaa ggttgtcagg tcattattca
     1201 tcgcaccaat caaactatcc gagacattta atgcttggtg ttgtaaggtc gttttatctg
     1261 gctcattaat cgattgaacc atgatttgtc ggaacatcgg ataccaaaag tatgctgtta
     1321 aaagaatcgt cattcctact gcctttgaca actccacaat caaccggatg ctttcttttt
     1381 ttcttgtttc acttactaca aagccgccta agactagtaa gccaacggct attacactca
     1441 taaaagtgga aagaacatgt gtataaatga gtaagctcat actgactgct aatggaattg
     1501 cacatggctt tttctcataa atcagtgcgt aagcataata cataactatc ggtataaccg
     1561 taagtgcaat cgcttcagca atagcagaac gatgataaat atcaatcgta cggtacccat
     1621 aaaatgtata taaacagcta aatataaaag ccgcatcgat tctttttaaa aattttagtc
     1681 cataatgaaa catcagcatg atagtacaaa cattcaatag ccaaacatat aagatataac
     1741 taacgattaa attattactt atccaataaa aaatgactgc cggaaaaaag gtcaagtagg
     1801 gataaaaata attcactccg ctcccataat gattaaacgt cgtaaaattg attggaccag
     1861 aaaaaattga agataaccct ttgactctag caaaatggaa ggcaatatcc cccgatgtag
     1921 aaatagtatg aacatttcta aaataattga ttgaataaag tccagcaata attgctagga
     1981 gaatggacaa tattattttt ttctttactt gcattactta attatatact cctttcatgg
     2041 attgctttat ctagtataac ttaaatttta aataaatcgt tatttatttt tactctcact
     2101 taaaaaagca ctaaaattat aagagaggga ggtaatggtc tgaacaccaa gaattgttcc
     2161 aacatcgaat tgcttaacta atgagttcaa agcttaatta tgtggctctt atatttaccg
     2221 agcgtggatt gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa cagccatatc
     2281 cgctttcttg gatatgacta tttactacta ctttactatc tttcattctt cttgtctcac
     2341 ttatgataag gcgctgcgta tcatgagtga atgatattag gaaaacttca taaaataaat
     2401 cttttataca tttccacttc tagatggtca ttcatgttag aagaatcaaa ctattttttt
     2461 act
//
LOCUS       DAHUWM010000267         2441 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHUWM010000267 DAHUWM010000000
VERSION     DAHUWM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.273"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            137..2290
                     /locus_tag="NK788_002664"
     CDS             137..2290
                     /locus_tag="NK788_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCH6770954.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          833 a          427 c          541 g          640 t
ORIGIN      
        1 tgattgctgt gaaacaaaaa gttgatttta tcttgaaatt aggtaaagaa tttcttgctc
       61 cgcccatttt ttctttttta ttaagaagtt tttgggtaca atgaagcatg aatgtaaata
      121 ggcggaagga taggaaatga tgaaagattt tatgaaaaaa atattaaata cttcttggtt
      181 agaaaaaata agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt
      241 tccttttcgc ttaaattttc tttttttcat tatatttacg ttatttgttg cgttgattgc
      301 tagattgggt tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac
      361 ttctacgatt acggtccaag aaagtactcc tcgcggaatg atctacgact ccacaggaaa
      421 agctttagtt acgaataaag cgaatcaggc catcacatat acgagaggaa cacaagttac
      481 ggctaaagat ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga
      541 tgaaaatctg acagatagag ataaaaaaga tttttggctg gctgatccca aacatttgaa
      601 agaagccaca aaaagacttt cggcaaaaga aaagcaatta gggaacagcg aacaatatag
      661 tgcgatggtc aataaagtca cagctgatga gattaattta aacgaggagc aattaaaaat
      721 tgctaccatc tttaaacgga tgaatagtgc atatgaatta aacactgtct atatcaaaaa
      781 ctctggtgtt actgatcaag agttagctgt tgtggcagaa cgcgcttctg aacttccagg
      841 agtttcgact ggcacagact ggacacgaga atataatgca gcaagttcac tgaaaagtat
      901 tcttggcagt gtgacgactg aaaagcaagg acttccagca gatgaagcag agaaatacct
      961 agcaaaaggt tattctcgga atgaccgtgt aggacaaagt tatttggaaa aacaatatga
     1021 agatgtcctg caaggaacca aaacccaata tgaagtttcc cttgataatg aaggaaatgt
     1081 ttcgaatcaa aaagaaatct tctctgggga aaaaggttct aatttaatgt tatctatgaa
     1141 tgcagaattc caatcaaaag tagaagaaat tttgaaaagg aattatcaaa cattgatcaa
     1201 caacggcaaa gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg
     1261 agaagtgcta gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa
     1321 tgctttaggc acgattacta gcgcctttga tccagggtct gtagtcaagg ctggtacttt
     1381 gacagcaggc tgggcaagta acgcgatcag cggaaatcaa gttttgattg atgagccgat
     1441 tcgtctgcaa ggtgcttcag aaaaaagctc tgtctttaat cgaagcggac aagttgctct
     1501 tgatgcagtc aaagcatcgg aattgtcttc taatacgtat atgatcaaag tggcgttaaa
     1561 gatgttagga ctagactata cacctggaat gggactacct agtttagatg aagaagctaa
     1621 agcttatcag caattgagag actctttcaa agagtttggt cttggtacaa cgactgggat
     1681 cgatttgccg aatgaatctc ctgggatctc acgttctgtt gattatatga agaagttcaa
     1741 tgcggataat ggcaaagaat ggtatgctcc aggtaacttt accgatttgg catttggaca
     1801 gtttgacaca tatacaccaa tccaattagc tcaatatgct tcaacagtcg ctaatggcgg
     1861 gaaacgagta cagccgcacc tagtcaaagc gatctatggc aatgacgaaa atggaaactt
     1921 gggtgaagtg aagaaagaaa ttggcacaac agtagaaaat acggtcaata tttctgcaga
     1981 aaatatggcg atcctccgtg aaggattcca ccaagttgtc catggtaccg atccttacac
     2041 aacagccaaa ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga
     2101 aacggttgcg gaaggtcatc cagatattgc gacagtcaat agtaatatcg tggcatacgg
     2161 accaacagac aatccggaaa ttgccatcag tgttgtcttg ccgaacttat tagacgaaca
     2221 agaccacata aacttgacga ttgctaaaga aataatggac gcgtattatg atatgttcat
     2281 ggcaaaataa aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt
     2341 gtgaaaatag cttctcacat ttttgcttaa atttgacttc ataccgagaa actggctttt
     2401 gaacaccgtt tactcggcaa aaaaggttgt gacaaaaatt g
//
LOCUS       DAHUWM010000268         2423 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHUWM010000268 DAHUWM010000000
VERSION     DAHUWM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.274"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            37..222
                     /locus_tag="NK788_002665"
     CDS             37..222
                     /locus_tag="NK788_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HCH6770955.1"
                     /translation="MEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNN
                     YQFGNLSYEMISKVLSL"
     gene            229..741
                     /locus_tag="NK788_002666"
     CDS             229..741
                     /locus_tag="NK788_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770956.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            984..1598
                     /locus_tag="NK788_002667"
     CDS             984..1598
                     /locus_tag="NK788_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770957.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1622..1972)
                     /locus_tag="NK788_002668"
     CDS             complement(1622..1972)
                     /locus_tag="NK788_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCH6770958.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1966..2229)
                     /locus_tag="NK788_002669"
     CDS             complement(1966..2229)
                     /locus_tag="NK788_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCH6770959.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          864 a          373 c          373 g          813 t
ORIGIN      
        1 accgaattta agttagcact ttatgcacaa ttttttattg aaaaattaaa taggtttcaa
       61 gaaatagttg aagatagtga ttttaacact tttaatagcc ataaaccgat gagaggtact
      121 tatataaaat ttctcgaaaa ttataatgat ttaaaatcta ccaataatta tcaatttgga
      181 aacttgtctt atgagatgat aagtaaagtt ctaagtttgt gatgataaat gaaattaaat
      241 agtataaatg atttatttgc tattataggt gttattgcta acatactttt ttcgtgtttg
      301 aattttagat ttactcaaaa gaacaaaaag aacaaactca gtatagaaca aatgaaaaaa
      361 gacattgaaa ctgatttaaa aaatgaccat aaagaaagaa gaaaaattga agaatatact
      421 tctttaatgg aaaagtgtaa agcaactgat gcaattttta gctttttaag aaaaagtgat
      481 gagataatca cacgtgaaaa tatagattta gaaaatgaga ttagtaaact tgaatctatc
      541 ttttccgata taaattctct taagtattta atcgtcgaag aaaattcgga tagcttttta
      601 gaatttgaaa aatatctaac agatcttata gataatagaa ataatgaatt aatgagacaa
      661 aacattgcaa gattccttac taaattagtt tctgaatgga aaagtattat ttctcaaaat
      721 atggataaat taagatatta aggatgttag ttttaaaata aactaatgtc cttatttttg
      781 tgttcacatt tctgttacct aagaaatact taaaggaggt gatgtagcca gcaacgacta
      841 caacttgaac ccgctatcta cagtttattt gttgtttctt ttgggtatac cctaactaga
      901 cacgaccgcg acattgaaaa atctggtttt ctcgtgtatg attagggtgt tttttaaata
      961 gttgcagaaa gtaggtaaaa attttgagag ttgcatacat acgtgtctca tccatcgatc
     1021 aaaatgaaca acggcagatt gaagaaatga aaaaattcgg tgcagagaga attttcattg
     1081 agaaacaatc tggcgcaacg attactcatc gacctgtttt tcaagaagca ctggatttcg
     1141 tccgagatca agatattttt attgtggaag ctattgatcg acttggaaga aattacgacg
     1201 agatcatcgc ttcagtcaat tacttgaaga agaaaaatgt gcagctgatc atcacaagtc
     1261 taccgatcat ggccgaagca attggcaatc ctctcctgga taaattcatt aaagatttga
     1321 tcattcaaat tttggccatg atcgctgaac aagaacgtac cgaatctaaa cgacgtcagg
     1381 cacaaggcat taagattgca aaagcgaacg gtgtttataa aggacgtcct aagctataca
     1441 gtgccaatgc aaaagatccc caacgtcgtc ttgtttataa aaacatcgtt gaggatctaa
     1501 aaaaaggtgt tgcgatcgct aagattgcaa aagactacaa tgttacgaga caaaccgtat
     1561 atagaataaa aaaagacagc atggtcaatc acgaatagtt gatcatgctg ctgtttctat
     1621 tttatttgct tactttttga aaaactgttc taactttcat caataactcc tcaactaaac
     1681 tatcatgcgc tttttccaaa aaaatacact ctctggcttc ataatcaatt gtcgttagtt
     1741 gatctaataa tacttttcct gttgtcttca tttcatattc tgagtttata tttacatata
     1801 gcggatagtt tctagttgta ctagaaattg gagcaactgt aacaacgtta ctataatccg
     1861 caataatatc atgacttaaa acaatataag gtcttttacc tgtctgttcg tgtccagatc
     1921 gaggtgctgt attcaataat agaatatcgc cttgtctagg ttttctcacc atttttcatt
     1981 acctactgct tcacctaaat tttgaatcgt cacatgattt ctttcatcag tgtaattttc
     2041 aaataactgt tcaattgtta attcattttc tatattagcc gcttcgataa ttagccgctt
     2101 ctgaccgttt acttcttctt gtattatctt cactttgtct tctgtatgaa ttccaagata
     2161 ttccataata tactttggca atctaaggcc attactattt ccccattttt ttataggtaa
     2221 catttccata gtagacacct ctttctgtat atacatagta taacatacat aagaaaaaac
     2281 gtatatactt aattatcctt ttctattaaa ttagattaac attccatgaa tccataaact
     2341 atgttaacat ttatatgaaa atagtcgaaa ggaaatcata atatgtttaa gaaaacaaaa
     2401 tctttagaat caggtaagaa aaa
//
LOCUS       DAHUWM010000269         2403 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHUWM010000269 DAHUWM010000000
VERSION     DAHUWM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.275"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            134..484
                     /locus_tag="NK788_002670"
     CDS             134..484
                     /locus_tag="NK788_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6770960.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            898..1716
                     /locus_tag="NK788_002671"
     CDS             898..1716
                     /locus_tag="NK788_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCH6770961.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1789..2148)
                     /locus_tag="NK788_002672"
     CDS             complement(1789..2148)
                     /locus_tag="NK788_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCH6770962.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          795 a          348 c          446 g          814 t
ORIGIN      
        1 tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga tgggtataag
       61 tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag tgtaacataa
      121 atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag caatcattcc
      181 tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa ttgtcggttt
      241 tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt ttattggtaa
      301 gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa aaagaattga
      361 tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat ttgaaattga
      421 atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt tagtaaaaaa
      481 ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat ttttattttg
      541 atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca ttcagtaata
      601 aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac cagttataga
      661 aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag gctgacctgt
      721 gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt atgtatctag
      781 atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa taggctgctt
      841 taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag tgtttttatg
      901 aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac atttgctcgt
      961 actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat gaaagctgtt
     1021 gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc gttatttcca
     1081 aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa agataaaaca
     1141 gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct tgatttatgt
     1201 aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta ctgtgaaaga
     1261 ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag attagaatgg
     1321 gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac tatgcctgtt
     1381 gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct attagtacct
     1441 accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa aactttcggc
     1501 ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc ttttggagat
     1561 gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat ggataatgca
     1621 acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga agacggtgtt
     1681 ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc atcaaaaaac
     1741 ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt acgaagcgtc
     1801 tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat tctttaaacg
     1861 aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa aggtagtatc
     1921 gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat atgctggaat
     1981 ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc cctctgctga
     2041 agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta aacgaggtac
     2101 ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt tcacgaccac
     2161 catcgacttc cacagttgaa attgacattt tatttaacac acctattccc ccttaactat
     2221 aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc aaatcgccaa
     2281 tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg taaaaattaa
     2341 accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt ccttagtcta
     2401 tta
//
LOCUS       DAHUWM010000270         2392 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHUWM010000270 DAHUWM010000000
VERSION     DAHUWM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.276"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1346
                     /locus_tag="NK788_002673"
     CDS             <1..1346
                     /locus_tag="NK788_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770963.1"
                     /translation="FKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLY
                     YAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKF
                     VVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNND
                     ITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISL
                     LQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKV
                     IQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYK
                     AADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKI
                     EITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1333..>2392
                     /locus_tag="NK788_002674"
     CDS             1333..>2392
                     /locus_tag="NK788_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770964.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLI"
BASE COUNT          791 a          367 c          409 g          825 t
ORIGIN      
        1 tttttaaaaa gatagatgct gcttttttat ttagttgttt atatactttt gcaacttatc
       61 gaacagttga tatctattat cgatccgcaa ttgcagaagc aatcgctctt acgattatcc
      121 cagctgtttt atattatgct tatggattga tattcaatag agaaaaaaaa gtcatcgctt
      181 tatctgtcag tataagtcta cttgtgtata ctcatgtctt aactacacta ttgtgtagtg
      241 cattgattgc ttgtatgatt atagttcgtt tgtttttaag caagcaaata aaaaacgata
      301 taaaatttgt ggtcgtgaat ttagtaaagg ctgttttttt gacttgtgca ttaactagct
      361 atttttggta tcctatgttg cagcagatga attatcaaga aatacacagt cccttcaaaa
      421 cagatttgca gctagaagca ctttctattt cagatagtat cattcaagcg ttaaataacg
      481 atattacaac atacactatg gggttattag gattggttgc gttgattttt cctttattca
      541 tactgaaaga aatggagaaa aaagaaaaaa tcatctatgt tggcgtagtt tctacatggg
      601 tatgcgtaac taatttattt ccatggtttt tgtttcaaaa aacaccgata agcttactcc
      661 agtttccttg gcgtatctta ggtatccaaa tctttttttc ttcaatgatc ataacgcttg
      721 tttttatgaa aaagactatg aataaaaaat attcatggtt aatcattggc gctactgtgg
      781 tgtttctact cgtcacgtct atagcagcaa aagaaaatta cgcaaaagtt atccaatcaa
      841 aacacggtca tattgttatc aatgaagaaa cactcccacg ttatacaaca tcagatatgg
      901 gaggattata cgattacgcc ccaacagaag cgataaaaga aaggcctcac ttagaaaaac
      961 atgaagtaaa agtaggagaa tcgtgggaaa aagggaagta taaagctgca gataataaca
     1021 ttacttacac agtggcaagc aacaaaaagc aaaaagtgac agtaccagta tatgcttatt
     1081 taggtacaca ggtaaaagta aacggtaaag tcgtaaatga ctcttataaa aaacatggct
     1141 tagtgaatgt ggatgtaaat gctggagaaa ataagataga aattacaaca aaatatactc
     1201 caactgctct catggcttta tggatcagta ttctgactta ttgtttagtg gtgtattcaa
     1261 gttttaaacg atttgcacct aataagttga aagattctcg atatgtgact aaaaaggaga
     1321 taaacaagaa gaatgaaaat aaataaaaaa gtagtaatat tgagtggatt aattatagtt
     1381 agtagtatat attctttcat ctttttcaaa aatacacaga gtatttatac cactggagat
     1441 ctttcttttc acttatctcg aatcaagggg ttatcgacta tttttaccag tccgattaat
     1501 tatgagacat tcaattatac tggatatggt gtaaattatt tttatccctt tctgactttt
     1561 tttccagctg taatgttata ttggatgaca aaaaatttga ttgtaagcta tattatttac
     1621 gtttggttat tgaacttgtg tacaacactc gtagcttatc attatggcga gcggttttta
     1681 aaacaaaaaa aagcggcatt tttatttagt tgtctctata ttttctctgc ttatcggaca
     1741 gtagacatct actatcgttc cgcaattgcc gaagctatcg caataacact tgttatccct
     1801 gtattatttt atgcttatca aatcatctct ggaaaagaag aaaagtatcc gtctgtaaag
     1861 ctagcattga gtatgagttt gctagtgtac agccatgtac tatcaacatt aatgagtaca
     1921 gcattaatta tcatttttat ttttattcgt ttggtttcta aaggtttcaa gaatgcagat
     1981 tttattgcta tatttaaaaa attatttagt gcggcaggaa tgacattggt tctgacgagt
     2041 gctttttggt atccgatgtt tgaacaaatg ctctaccaaa aaatcaacaa acctagcgtg
     2101 acaaatttat atgctcatgc atctaatgtg ttcgatagtc tgactgaagc tatgaacaac
     2161 gacttaacta cttactcaat gggactagtt ggtctgttat ctttatgtat tcccctgatt
     2221 ttatttaaaa aattaacaaa catagaaaaa aagatttatt atggtacgtg tttgacttgg
     2281 ctagctacaa ctagtttggt gccatggtat cttctgcaaa atacgccagc caaattgtta
     2341 caatttcctt ggcggatctt aagcttgcaa ataatattct cttctctgat at
//
LOCUS       DAHUWM010000271         2359 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHUWM010000271 DAHUWM010000000
VERSION     DAHUWM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.277"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            456..1949
                     /locus_tag="NK788_002675"
     CDS             456..1949
                     /locus_tag="NK788_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCH6770965.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2084..>2359
                     /locus_tag="NK788_002676"
     CDS             2084..>2359
                     /locus_tag="NK788_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCH6770966.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRK"
BASE COUNT          809 a          368 c          479 g          703 t
ORIGIN      
        1 atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga gtgaaaagag
       61 gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag caaaagcagt
      121 ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt gattttagca
      181 gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc tcaagaatat
      241 gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc gattgccttt
      301 attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg tacttaaatt
      361 aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa gctaaactgt
      421 gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt tattcagcta
      481 ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa cctgcaggtc
      541 atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa ttaatgaatg
      601 ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa aagaaactaa
      661 cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat cgaacagtgg
      721 ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt gatatcgatt
      781 caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg ggctacatgc
      841 caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta aagacaccag
      901 gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa atttcaaaaa
      961 caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt aaccacttcg
     1021 gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac tatcagtatt
     1081 cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa acaaaagaag
     1141 atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa gtagatgaac
     1201 cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga cgtcttggtc
     1261 gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat gcattggata
     1321 cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta tctgaagcag
     1381 aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca agcagagaat
     1441 tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat gatctgttca
     1501 ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa tatagtcata
     1561 aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt gctagtgagc
     1621 cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc aagcgaacac
     1681 ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag gtaacacgag
     1741 gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg gttattcaac
     1801 taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt ggactcaacg
     1861 agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct cttaaagaag
     1921 tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc cttatacaat
     1981 atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta ggtggtatac
     2041 ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt taaaaaatta
     2101 tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat ctgtggaagt
     2161 gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta ccagggaaaa
     2221 aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt ctggggagac
     2281 tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg agtataaaat
     2341 agctaacgag ttaagaaaa
//
LOCUS       DAHUWM010000272         2344 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHUWM010000272 DAHUWM010000000
VERSION     DAHUWM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.278"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            18..>2344
                     /gene="esp"
                     /locus_tag="NK788_002677"
     CDS             18..>2344
                     /gene="esp"
                     /locus_tag="NK788_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCH6770967.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          850 a          370 c          481 g          643 t
ORIGIN      
        1 gggagttgat ttgaataatg gttagcaaga ataataagag agtatttcta gaaaaaacaa
       61 agaaacgagt attgaagtac agtatcaaaa aattaagtgt tggggttgca tctgttttga
      121 ttggtgttgg tcttgtcttg ggaacaactg aactagttaa agcacaagat gaaatttctc
      181 caagcagccc acttgaaaca gcgataagct ccgtacaagt aggtgacaaa gtagcttcgg
      241 ggaatacttt tcaggagaat cctggttata cgaaaaatta taacttttcg gatttacaat
      301 ttagtcctca agaactaact ggagacacac taaaaggcaa tacaattgga tttgaagttt
      361 acggaaaaca taatattgca gcatcaacta aaaactggga aatccgtctt caattagatg
      421 aacgattagc taaatatgta gaaaaaattc aggtcgatcc aaagaaagga attggatctt
      481 ctagacggac ctttgtaaga attaatgatt cgctgggtag acctacaaat atttggaagg
      541 tcaattatat tcgtgcaagt gatggattat ttgctggggc agaaacaacc gatacgcaaa
      601 ccgctcctaa tggggtaatt acgtttgaaa agagcctaga tgaaattttt aaagagatag
      661 gcatagacaa tctcaagacc gatcgtttaa tgtatcgaat ctacttggta agtcatcaag
      721 acgatgataa aattgtaccg ggtattgata gtacgggcta ctttttaaca gattcagatg
      781 atttctataa tagcttagat gtatctgaaa ataatcctga ccagtttaaa catggatcag
      841 taagtgcaaa atatgagaaa cctaatactc aaacaaaaga tggttcagga tccacaggtg
      901 ctaatggagc tataatctta gatcataagt taacgaaaaa ttataatttc tcatattcat
      961 cttccgcaaa gggcacgcct tggtatgcta attacaaaat tgatgaacga ttagttccat
     1021 atgtagcggg tatacagatg cacatggttc aggctgacaa agtaacatat gatgtttcgt
     1081 ttgaatccgg aacaaaagta gctgatttag caatcgagcg acgggaaggt catgaaaatt
     1141 atggcatagg ttcaataact aataatgatt taactaaact tatcgatttt gctaatgcaa
     1201 gtccacgtcc agtcgttatt cgatatgttt tacaattgac taagccatta gatgaaatct
     1261 tagaagatat gaaagcaaca gcacaagttg aagaaaataa accatttggt gaagatttca
     1321 tctttgattc ttggttgtcg gatacgaata aaaaattaat ccagaacact tatggaacag
     1381 gttattatta tttgcaagat attgatggtg atggaaaccc tgacgataaa gaagagagcg
     1441 gagacacgaa tccatatatc gggaaacctg aattagaaga agtatatgat gttgacacaa
     1501 cagttaaggg gaaagtattc atccacgagt tagcgggaac aggtcacaaa gcccaacttg
     1561 ttgataaaga aggtactgta ttagcagaaa aaactatcgc tccaaatgaa aaagatgggg
     1621 ctccaatttc agatactgta gaatttgaat ttacgggtgt agattcaagt aaattaatcg
     1681 cgaaagatga attaaaaatc caaatcgttt ctccaggttt tgataaacca gaagaaggtt
     1741 caaccgttat taaggaatca ccaaaagcgg ttgataaaca aaccgtggta gttggattta
     1801 aaccagatgc taaagaatca attcggaata ataaaaactt acctgaagat gcagagtatt
     1861 catggaaaac agagcctgat acttctaacg ttactgatag tacgaaaggt attgtaactg
     1921 ttaagatcgg aaatcgaact ttcgacgtgg atgtagagtt tgctgtaaaa gcttctcaag
     1981 ctatggaaaa tgatgcaaca tacgtaccta taacaacaac tccagaaacg acagttcaaa
     2041 gtggaaaacc tacatttgat aaaccagatg ttcctttagc taaagatgcc ttttcaattt
     2101 cagatgtcta taataagtac ttcggtaatg caagtgttga tgcaaacact ggtattgtta
     2161 cattcactcc agctaaaggt gtaggagaat cggagccgat tactggaaca attcctatta
     2221 aaattgttta ccaagatggt tctgtaggca cgaccgattt agcagtaact gtaagtaaag
     2281 atatttatga aaatccagga gaaaacattc ctgcaggcta ccacaaagta accttcaccg
     2341 cagg
//
LOCUS       DAHUWM010000273         2336 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHUWM010000273 DAHUWM010000000
VERSION     DAHUWM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.279"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            324..941
                     /locus_tag="NK788_002678"
     CDS             324..941
                     /locus_tag="NK788_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770968.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            982..1281
                     /locus_tag="NK788_002679"
     CDS             982..1281
                     /locus_tag="NK788_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCH6770969.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1315..1482
                     /locus_tag="NK788_002680"
     CDS             1315..1482
                     /locus_tag="NK788_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6770970.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1527..2132)
                     /locus_tag="NK788_002681"
     CDS             complement(1527..2132)
                     /locus_tag="NK788_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770971.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          790 a          369 c          434 g          743 t
ORIGIN      
        1 agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa ataaaatata
       61 ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag accgttaagg
      121 cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg taaacttact
      181 gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta tggcataata
      241 accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa acaattttag
      301 tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc agtagttatg
      361 atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct gaagaatata
      421 tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac
      481 ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta ggcagaaata
      541 aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt gatatttctg
      601 tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg ttagagacgc
      661 ttatctctga cttaattctg caattactca gctatatggc cgaagacgag cggaaacgaa
      721 ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca
      781 gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa
      841 agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc ttttatcgac
      901 gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa cagctattag
      961 acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt tactggaacg
     1021 ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa cggccattgt
     1081 caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc taagtcgttg
     1141 tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat tgcgttaggt
     1201 catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca acagttgaaa
     1261 gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat ttttatgaag
     1321 cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa tattgctttt
     1381 cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa ttacttttac
     1441 tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa tagtgcggta
     1501 gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat tttataaata
     1561 gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc ttgctttaac
     1621 atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt aatagaatac
     1681 tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc ttgccgctga
     1741 cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga taaaaacata
     1801 tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa ctgtgcatct
     1861 aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt tatatcttca
     1921 taatctcttc ctaaacgatc tagtgatgta acgatgacgc aatcaccttc acggatataa
     1981 tttagtaatt tttgaaactc aggacgctca gtatctttac cactggcttt ttcttggaat
     2041 aatttatcaa tgttaagtcc ttccatcatt gccaattgtc gatccaaatt ttgatcttgt
     2101 gaagatactc gaatatagcc aatttttccc ataaaaaact cctttttaaa agtgatataa
     2161 tttacatgta aaatttactt gtagttttac tgctatcata gcatttttaa aaataaattt
     2221 tatgtagact taaaatttac ttcatggagg gtttaaatgg atgaaatcaa aagtaacaaa
     2281 aaagcatttg gcatttattt aggagaacct agtacagaac aattaaacca attttt
//
LOCUS       DAHUWM010000274         2322 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHUWM010000274 DAHUWM010000000
VERSION     DAHUWM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.280"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            69..443
                     /locus_tag="NK788_002682"
     CDS             69..443
                     /locus_tag="NK788_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770972.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            448..876
                     /locus_tag="NK788_002683"
     CDS             448..876
                     /locus_tag="NK788_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCH6770973.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            967..1881
                     /locus_tag="NK788_002684"
     CDS             967..1881
                     /locus_tag="NK788_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCH6770974.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            1997..>2322
                     /locus_tag="NK788_002685"
     CDS             1997..>2322
                     /locus_tag="NK788_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCH6770975.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPET"
BASE COUNT          889 a          365 c          400 g          668 t
ORIGIN      
        1 ttttattagt tgtcaacacc tttaactaat ttttttatta gtgttttcgt agcttgtgag
       61 gtgcgaaaat gaatttacta gatagaataa aggaattagc tcataaaaga ggcataagta
      121 taactcaatt ggaagaagaa ttaaatatac ctaaaaacac gatttatcaa tggaaaaata
      181 gaactcctag tactaaaaga ctacaacttg tagcagatta ttttaatgta actactgatt
      241 atttactcgg tagaaaccaa gttcctgact gggcaacaaa agatgaagta gttgaacttg
      301 ataaattact agattcaaat gtcaatatgt cttatggcgg agaaacactg acgcctgaac
      361 aggtccaacg cgtaaaagat atccttattg gaaccttctg ggatattgtg aaagaagaca
      421 aagaaaaagg caaaaagatg tgagcttatg gagatggata cgattaattt agtcgaggag
      481 ttgaagcgga aataccagtc cgctaatcct ttttatattt gtgaaaagat gggcattaaa
      541 attcaatacg ttccttttat cgagaatccc aaaggacagt ttcaagaaat tagaggtcgt
      601 gcaataatct tcttaaatga tgaactgaga gactctgagg aaagattcta catttgcgct
      661 cacgaattag gtcacgctat ttttcatcgt ggcttatcca gttactatgt atcaacaaga
      721 acatctagaa gcaaatctga aagcgaagct aattgctttg ctgctaatct cattgtttct
      781 ctatacaaag aagacaatga tcaatatcct aaacgaattg aggaattaaa gaatctttac
      841 ggacttccag aaagcgctta tcgctttctt atataatgta aaaaaagtcc gtgctgcaac
      901 acggactaaa aaccttattt ctaagatcct acaaaaaaat catatcatag aagtgaggaa
      961 ttaaaaatga aaaaaattgt ttttgggttg ctttttttag gcttggtatt aagcggatgc
     1021 tccactagtc ctaattctgc tagcactaaa gaaaatactg tttcatcaac atctgaatat
     1081 gaaacaaaaa aactaacttc atcagacgtt cataaaataa aagttggtga tgatttaact
     1141 caagtttatc agacacttgg ttcaccgtta cgtaaatgga caaatgattt tgtttacaaa
     1201 gaattagaca atgctatcac acgggatgaa ttaattgtat ctatttcaaa ggatgcacaa
     1261 ggagattatt ataaagagca ctctgaatta attgaacatg gaaatagtgc aaaagatata
     1321 aaaaaattag agatgctcca atatacttat tcaactgatt ctgatgatga aacaatgtta
     1381 atttgggtta gtccaaaaac tgaacaggtt gtatattata atcaacgaac tttcttagat
     1441 aaagatggaa aatccaaaga ttcaagctct actacaacta caactacaac ttcgtcttca
     1501 gaaccccaga cggcatcagt tgcacatagt ataggagaag cagttccttt tacttctgaa
     1561 aatggtaact ctattaacgt aactgtccag tctgtatcaa aagattacgg tgatgatttc
     1621 tataaaccag aaggcctatt ttacgcaaag gtagattttc ttgtagaaaa cacagggact
     1681 acaccttttg atgcaagttc aagttattta gaattttatg attcatcaga tattaaatct
     1741 gatttagcta gtagagatta cttttcggaa accatccaac cgggtaaatc cgcacaagga
     1801 acagcatatt ttgaagtgaa aaatgatgga acaacatttg aagtgttctt cgcagatact
     1861 tcatggaaag gtagctacta aacaaaaaaa cacgccccct ccctcgccaa agtttgtgga
     1921 cgtgatgaaa aataaacctg ctataattgg cttacttatc cattcctatt atagcaacaa
     1981 ataggagatg aaaacaatgt ggattgaaac caaaactgat aaaaacggaa aaaaagtata
     2041 taaatataat gagcgatata ttgatccaaa aactagaaaa agaaaaaaag tgagcattac
     2101 ttataaaaat aaatctcgag aaactcagaa agtggcattg ctagagttaa ataaaaaaat
     2161 tgatataaaa ttaaacgaaa aaacacttca gaagcctgat ctaacatttc atgagcttgt
     2221 cgaagaatgg ttagttattt acaaaagaca agttaaggag tctacatatt atcctacgaa
     2281 taatatctta aacactataa agaaaaagat acctgagact ta
//
LOCUS       DAHUWM010000275         2316 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHUWM010000275 DAHUWM010000000
VERSION     DAHUWM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.281"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            135..1997
                     /locus_tag="NK788_002686"
     CDS             135..1997
                     /locus_tag="NK788_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6770976.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1997..>2316
                     /locus_tag="NK788_002687"
     CDS             1997..>2316
                     /locus_tag="NK788_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCH6770977.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          827 a          345 c          457 g          687 t
ORIGIN      
        1 gtagtcgtct ttataataga taaactctgt aatattagtt aaaaagctat aagttgcact
       61 tagttaagtg caaacatttc atattgtcgt tagtttagaa aacgcttata ttagcaatgt
      121 aaggagagat gataatgcag cagctaacaa aaagagaaag gcagcttttg atttacttgg
      181 tggaaatgtc tgaaccggta acaatcaaaa agttggctga aaaagaaaat gttagtgttc
      241 ggacgataaa atacgatcta gatgatataa gagagtggct gaatgagcgg aagcaagtcc
      301 tatatagcaa acgtagtcag ggaatttgga tgaatattag tagctctgat cgaatcaaat
      361 taaaaagcga attgatggat gttaatcgtt ttgaaatatt tgcagatcaa agtctccgaa
      421 tcaaacgttt actaattatg cttttagtaa caaaagaagc tattactgga atgaaaatgg
      481 ccaacgcatt agatgtcagc aaagatacga tcatgaatga tttagatgtg ctggaaaata
      541 attttcttaa ggaaggattg accttgaatc gtcaggcgcg aaagggtttt tggatcatag
      601 gagaagaacg tctagttcga ttaacgattg aagagattct tcaaaaagag ttcacggatt
      661 atgacattta taaattaatg tcattgttat tgaatggcgg tgagactgaa tactttgaaa
      721 tgtattctgc tactgctaca cctatccaag aggtatttaa tcaagtaatc attcgaatgc
      781 gacacttact ggaatttgaa aatctagaga aactgaatta cgcggaatta ctaaatattc
      841 tgattcgagt gacaattgct accgttcgct tacgcaaaga ggctacgatt ggtcgttatc
      901 aattggttgc tgaacaagaa ttagacaaaa acgatctttc ttatcgcgtc atgcagcagg
      961 tctttgatta ttatcagttg ccactgttcg aggatgaata tcattatatc tatagtgata
     1021 cattcgacac gattcaccag caagacgtca tgaaactagt tgagaatgtg atttataaag
     1081 taagtaaagc aatcaaccat tctttttatc aagatccaca attgttgacg aatctgtatg
     1141 ctcatttatc catgcgatta tcgagaaaga aaaaatttgt caatgaatat aatcctttta
     1201 aagaagatat caagcagaaa tacccggaat tatttgatgc gatcaagcaa gcagttcgac
     1261 aagaaataag tgaacaaaat attctgatca atgattcttt cgtggcatat attgccttac
     1321 actttttggt ttcctacgaa agagaagaag atctacgaag cgttagagcc gtctacattt
     1381 gttcaacagg gttgggtgtg actagtctga tcaagcaaaa gatcaatgaa gaattaaata
     1441 atatcactat cgtggcattt gcttcagtat tgaatgcgca agaaatgatt gctaccaaag
     1501 atcctgattt ggtgattagt atttttccaa ttaatggcat cgatcgtcct ttcatcaaag
     1561 tccatccatt acccactgaa caggacttga aaaaaattca acaaataata aaaaaaatac
     1621 ttgcttcttc caatcagcag gaaaggcata acttgaatct tagagaaacg tccatcaata
     1681 aagatacttt agaagacttt agcaaggaat tgattttgaa agcttatacg atttatgaac
     1741 gtttacttgt cattttggaa gcagaattga tccaagaata tcgagaagct tttttgcttc
     1801 atgtattctt aatggttcat cgtatcacat ttgatcaaca atatatggca gaaggtcgtc
     1861 aagtagacca gattatttta gacaaaggaa cactggtagg aaaaatcgaa gcagtttttg
     1921 cagaaaataa tttaggaatc aatcagtcag aaatcagtgc attattctca tatataaaag
     1981 aaggagcgtc aagttagtga aaatgtcaga cgaggcttta aaaattatcg aggaaagtca
     2041 aaatcgggaa aagttaaaac aagtgattga tctaattgaa gagcgattag tagaacatgc
     2101 tattgtacca accgagttgc agtggaccat tctaattaat cacttgaatg agatgctgaa
     2161 acgttctcaa agaaaggaaa cgattcccgt agtcgatcgg gatttgttca aagaagtatc
     2221 caaagaagcc ataactattt ctgatgaaat agttagaagt atcggtaatt taaccgatga
     2281 tgaaatatat gttttgtcga ttcattttga aacagc
//
LOCUS       DAHUWM010000276         2299 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHUWM010000276 DAHUWM010000000
VERSION     DAHUWM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.282"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            135..1748
                     /locus_tag="NK788_002688"
     CDS             135..1748
                     /locus_tag="NK788_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6770978.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          718 a          407 c          447 g          727 t
ORIGIN      
        1 atttttaggt tttatccaca aattatttct ctatattttc tgtctattca aaataaacaa
       61 taattttccg gcacaattat ttgctgtttt ttaccataac atagtacgat caacgtagaa
      121 aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt agctggcggt ccttcatttg
      181 ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa cgcggagaaa
      241 atatttctta tgcaagttgt gctttgcctt attatttagg aggtgtgatc actgaccgtg
      301 actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc gatgtcttta
      361 ctaaacacga agtaacagca atcgatcctt ctactaagcg attaacagtt aaagacctat
      421 ccacaaatga agaaataaaa acagattacg ataagttgat catctcttct ggtgctagac
      481 cagattatcc ggatattccc ggagtttttg aagcagaaaa cggctttgtc ctccgtagtg
      541 tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa catgtcgtca
      601 ttcttggtgc aggtgttatg ggtctggaat tagctgagaa tttcaagcat cgcggcttag
      661 acgtgacttt aatcgatcaa ttgccacaag tcgctttccc ttatgatcca gaaattgcta
      721 atttagttta tgacaaattg ctgaaagaag gattagccgt tcatttagaa acaagagtta
      781 ctgagatccg tgataaaggt cgagaaatag tattatcaga tggctccgtc ctttctgctg
      841 atatgctaat ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa gcaaccggca
      901 tacaattatc agatacaggt cagatcattg ttgatgacca gttacaaacc aatctgccgg
      961 acatctatgc gattggcgat attatcgaaa cgactagtgt agtgactggt cagccgatcc
     1021 aaagtatgct ttccagtgcg gccaatcgtc aaggacatat gctggcagat attttaaatg
     1081 gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc tttgatcata
     1141 cagcaagtta tgcgggaatg acagaacatg aattaaaagc atcaggcata acaaattata
     1201 aaactgtttt tatcactccc tttgaccatg cctatttcta tccagaagct acaagattaa
     1261 atctaaagct gatttttgat gcagatagcg gtcgtatttt aggtggacaa gcatttggag
     1321 aaaaaggtgt cgataaacgg atgggagaac tttctgtagc aatcaccgga aacttgacag
     1381 tctttgattt gcctgatttg gagttgcctt actctccacc gtattctact acccgtgacc
     1441 cgttgaatat agctggttat gtcgcaatca atcaaatgac gaatatcgta gaaacgatca
     1501 aagcaagtga tatacccgaa aacgatttga aagaagcgtt ctttttagat atacgtgaac
     1561 ctaataaagc accttctggc agtatctcgg caacaaaaaa tattccaatg aatgagttac
     1621 gagatcgaat caatgaaatc ccaaaagata aaaaagttta cattactttc agaagaggat
     1681 tgaatactta tacttctgcc cgaatcttgg caggtttggg tatcaaagcg attttgattg
     1741 aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag gagttatttt tacttgagga
     1801 gtaaaaatga aaaagtatat agggttgttg gttggtatgt atatagaata gaagataaat
     1861 gtgaagaaat tgtgcattat ttcttcacat ttattaggtt atagtttaag aaagtttaaa
     1921 cattgcgcaa tttaattatt ttagctgttt cttttcataa aaaaacatgt tgtgactctg
     1981 agacaaatca tagtccatag tgatgtctct agagtaacta cgcctaatat tacgaggcat
     2041 catattaatc tatcagcact agtacctaaa ctagcatccc tttcgaatat tttttcttat
     2101 ataataaagt ttgctttcac tcctctaatc tcaccaaatc gactggcttg cgttgtgtga
     2161 tactaggaac tgattggttt agaacgactt ttctattgta atcatatttt tcgccatcct
     2221 cgttgtcttc taatccttca atgaatgatc cctaatgtgt gtattaaata atttaatttt
     2281 tttgttccta tttatttaa
//
LOCUS       DAHUWM010000277         2260 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHUWM010000277 DAHUWM010000000
VERSION     DAHUWM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.283"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..649
                     /locus_tag="NK788_002689"
     CDS             <1..649
                     /locus_tag="NK788_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770979.1"
                     /translation="YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLL
                     EKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQG
                     TYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKA
                     SSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(725..1060)
                     /locus_tag="NK788_002690"
     CDS             complement(725..1060)
                     /locus_tag="NK788_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770980.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            1187..1402
                     /locus_tag="NK788_002691"
     CDS             1187..1402
                     /locus_tag="NK788_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770981.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            1439..1801
                     /locus_tag="NK788_002692"
     CDS             1439..1801
                     /locus_tag="NK788_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCH6770982.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            1847..2173
                     /locus_tag="NK788_002693"
     CDS             1847..2173
                     /locus_tag="NK788_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770983.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          799 a          313 c          459 g          689 t
ORIGIN      
        1 atacctaaat aggttaaagg aagagaaagc gttaggaaac tttaatcata agagaagcaa
       61 tgtctatcga ttagacgttt ttaatgcaac ttctgtgatt caaaaattga atgaagatat
      121 ccttccactt ctggaaaaaa ttcattttac atatcaatat ttttcttatg aatgggttaa
      181 gatatgtttt aacgaaaaag aggctaaaag agtatttcct caaaattccc gatcagaact
      241 cagcaaggaa aaacaattga ttttagatga agcacatgaa gttttacagg gcttatcgtg
      301 gcaagggaca tatctaatga cagagaaaga acttattaac actgtttgtg aacgcctacg
      361 taatgttggg gataacagcg tgaaaaatgc tttaataaaa aacagaggct actttgttaa
      421 acaaggattc actcttgaac gagcttctaa ggatattaag aaactataca atgtgaaagc
      481 aagtagtagt tttttaatat atttagcgaa aggggcaggg atagatgaaa caataaaaga
      541 attgatatta tcaggagctg ggatagttcc tctgtatcaa tttacaagtg taacaagaga
      601 tgatggacgg agagtaagga agagaaaaga cccaattgta ttaaactaga caggttatcc
      661 atagctctta atcttatttc atctcaatac acccaccgtt aaagttcggt gggttattta
      721 tttttcaatc tttcaatagc tgattgcgtt cattttcttg cctttttaat agcttcatat
      781 agtcaattgt tcgaactgca tcttcagtgc taagagaacg atagagagcc aaaaactctt
      841 cctcataatc tacgacaaca ttgtctagaa aatggcttac aggggttcct agggctctag
      901 aaagagctgt taagctatcg aggttaggct ctgttacaga attttcccat cttgctactg
      961 cctgttgtgt agagaagatt ttttcagcta attctttttg tgtccacccg agagcttttc
     1021 gcttttctct aatgaccttt gcatattgtt tctgtttcat taatacaccc ccatctcagt
     1081 atagtacaac aagttgttgt atttgtaatg gtatttgaag ttttttatat tttatgagtt
     1141 gaaatacatc atattgttgt atataataaa acaggagggg agaatcatga aaaaagtaag
     1201 taatctcagc cgatacagaa aagaaaaagg actttctcaa acggagttag caaaaaaaat
     1261 gaatgttaca cagcaatgtg taagttcgtg gcaaacaggg agaacgattc ctaaacccta
     1321 tcaaatgaag atgctttctg agatattaag tgtaccaatg aatgagctgt tttctgatgt
     1381 atttaataaa gtaaacagtt aactaataag caattgaatg attttaggag gagaagacat
     1441 gtattgtaga aaatgtggca aagaaataac agatgaaagt aagtattgcc agttttgtgg
     1501 agcactacaa gaaacagatg agacaaccgg aacagacaca gtaaagaaaa gtactgagca
     1561 gacaattaat gaaatgaagg aacaaatcgt acatggacaa gagacaggga agctgtattt
     1621 gattattggt tggatttcta tggtagtatc gttagcattt attcctgttg tatttggtgc
     1681 tgttgctgtt attatgggct acttatatcg agaaaaagat gagaaaatgg gtacgatttt
     1741 gatgatagca ggggtagctg gagcaatctt tggtgtactt ttagggatgt caatgtactg
     1801 ataagaggga taagagcaaa ttattttgga gataggagat aaaaagttga ataaagtaaa
     1861 tatgaacaag aagtttgtca ttataggtat tgttgtcttg gctattatag gtattatttt
     1921 ttcaaaaatg acaggtacat ctggattaga gaagtcatca aaaccattgg taacacagat
     1981 tttacaagaa cagtacaact taaatcgatc atgtgatgat gtgacgatta ctcatgaaaa
     2041 tggagataac acataccggg ctaaagctat attagacaat ggaagtgcta tcaacataaa
     2101 tattgagtac tatccaaaga aagaccgtat atatgtggag ataccatatg ctgaggtgtt
     2161 aatgttaaat tgatttaaat gtattatgca taccgtccaa acaggaactc ccaaaggtgt
     2221 aaattcacta gggagttttt atagtttcct ataaattaaa
//
LOCUS       DAHUWM010000278         2257 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHUWM010000278 DAHUWM010000000
VERSION     DAHUWM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.284"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            164..349
                     /locus_tag="NK788_002694"
     CDS             164..349
                     /locus_tag="NK788_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6770984.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            361..609
                     /locus_tag="NK788_002695"
     CDS             361..609
                     /locus_tag="NK788_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770985.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            640..819
                     /locus_tag="NK788_002696"
     CDS             640..819
                     /locus_tag="NK788_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770986.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(862..1332)
                     /locus_tag="NK788_002697"
     CDS             complement(862..1332)
                     /locus_tag="NK788_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770987.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1419..2144
                     /locus_tag="NK788_002698"
     CDS             1419..2144
                     /locus_tag="NK788_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCH6770988.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
BASE COUNT          839 a          321 c          443 g          654 t
ORIGIN      
        1 taataaacac ttcacggtcg gcaaactctg aaatgtaaat tactaggcat ttctgtgtct
       61 tatttagcta tgtctatata ctaataaaaa tattagttat tgtcaacgaa tattagctaa
      121 ttttttagct tacagattat ttttttagaa aggagctatt tttatgtctg agaatttaga
      181 cttaaaaatt cgagcggaga tgagaaaaag aagaatgact ttcaaagaac tagctgcgct
      241 tatcggtatt tcaggagctt atttatcaga tattctaaac ggcaatcgtg atggaaagaa
      301 agcacaacag catatcgaaa cagtgaaaaa aatattggac atccgatagg ggggtaggcg
      361 atgacaaaac taaaaaaaca agattttgta aaaaaataca attattctcc atctacttat
      421 caacgtcgaa tgtcggaact aaaaaataca gcaattttct cagcggcgta tgaacgggtc
      481 acaggacaag aagtttggat caatacagaa ttatacgata aatttttgtc tttcaaatcc
      541 tataacaggt tacgcacaag aaaggtaaca cctaaagaat ttatcgagaa gcatttagtt
      601 gatttataaa aaacagaaca ttttgagagg tgaaggttaa tgggtaaatt caacagagca
      661 ttggtattca gcgcaccgct aatcatctac gctttaggac tttggggaag caggcaagcg
      721 ttgataggaa cgattgttta catggtttgg atttttatgg ggcttgatga agctgaagct
      781 gagtacagag cgaaaaagcc aaccgagggg gctgactaaa aatgaataga aaagagaaac
      841 tagaatgtat attactatta ctcagtttaa ttctttcact aatttctcta ttgggaagtt
      901 tgtatttttg atatcaacaa ttttgtggat ttgggttcta tttagagtaa atatcatttt
      961 tgtttggtga ttaaggagtt tattaaaatt aattttccct acttcaaatg caaggtaaca
     1021 agatatagca cttttaggag gaatatttag cggtacagct tcagagtata ttctattatc
     1081 atcgactgat cctcctttag caattaattt cttaaatgga gtagctgacc atctgcggtt
     1141 taagttagcg gatgaaagtt ctaaattaac taacatagct ggttctgttg aatagttaga
     1201 aatgattact tttgtataaa tcatatctgc agcgaagtaa gaagcattaa gttcaacttc
     1261 tagttgaggg cgcattcctt ttatttttat ccctgttaaa aacgtactca atataaaact
     1321 tattattgac atccattgaa aaattgttaa gtgaaaaaaa ttcaaaataa acaccaccag
     1381 tttttaacta aattatacca aaaaggagag aagaaataat gcaagaatta gtaattttga
     1441 aaaataaaga agctgtaact acgagcttac aagtggcaga aagtttcaaa aagaaacata
     1501 agcatgtact agaagcaatt gaatcaataa aaagatcggt cgaaaattcg gccaatgttg
     1561 aagatgggtc caattttgga cagatgtttg tggaagggaa cgagccagac tcatacggaa
     1621 gaagtcggag agtttatttc atgaatagag acggattttc cttgctagct atgggattca
     1681 ctggaagtaa agcaataaat ttcaaactaa aatttattga agctttcaac gaaatggaag
     1741 atgttattcg gaagaatact gttcctcaaa caattgaaga catgatgatc tatcaattag
     1801 aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa agatactatg cgaattagcg
     1861 gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa agttatggaa gttctagggg
     1921 gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg
     1981 aatttaaacg taccttttca atcccaagat atggcgagtt acctcgtaag agattcgatg
     2041 atgctgtttc atttattgaa atgtggttac cagaaactgc gatccgcatg gaaatcgatc
     2101 aactgaacag acaacaaaga cttttcggtg atgaaaatga atagagctga agcgctaaga
     2161 atagggacgg taattgctaa tcgctggtgg agacacaata aaccaaacat cctaagccaa
     2221 caacatatcg aaaagcaaaa agcatggcaa caaataa
//
LOCUS       DAHUWM010000279         2241 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHUWM010000279 DAHUWM010000000
VERSION     DAHUWM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.285"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            115..2172
                     /locus_tag="NK788_002699"
     CDS             115..2172
                     /locus_tag="NK788_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCH6770989.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
BASE COUNT          807 a          399 c          404 g          631 t
ORIGIN      
        1 aaataagaaa agccaaaatt atataaggct tttcttattt tttattgact atatcggtta
       61 accgatatat aataatcatg taaacatttt taattaaatt taggagttga aaaaatgaaa
      121 ttatttaatc caaaaactgt tttagctatt ggaacatttg ctgcctcact tagtcttgct
      181 ggatctgttg acgctgcttc aactaacgtc atgtatcgcc tttacaatcc aaataataat
      241 gaacattttt acacctctag caaaaaagaa cgtgaccatc tagtaaagat aaaatgggga
      301 aattatgaag gccccgcctg ggaagcaccg actagcggtg gccatcttgt ctatcgtttg
      361 tacaataaag gtcttcgtga tcaccattac acagctagtt gggatgaagt gaaatggttg
      421 actaaaaatt acggctggac ttatgaaggg cctgcctggc gttctgccga aaaaaataat
      481 aaaccgatct accgcctttt ccttcctggt gtaaaatctg gctcacatca ctacactgca
      541 agttggaaag aagtacaatg gttaaccaaa gactatggct ggaaatacga aggtatcggt
      601 tggtatggcg ctgacacctc taaacccaaa gtgaataaat cggctctaga agctctttat
      661 acgagcgtta agggaacgaa aaaaggtgac tataccgaca atacctggaa cgcattccaa
      721 accgctttaa acaatgctaa gaaagtgtta aatgactcaa aagcgactca aaagcaagtg
      781 gatagtgcca aaaataaatt agattctgct tacaaaggat taaagaaaaa accagttcct
      841 actgtgaata aagctgaatt acaagcttta tacaaccgag taaaatcaac tgctaaagga
      901 gattttacag aagaatcttg gaataatttt cagactgctt tatccaatgc taaaaaagta
      961 ctagacgatt ctaaggtttc tcaaacacaa gtaaacaatg ctaaaaatag tcttgatgct
     1021 gcatttaaag ggttgcaaca caaacctgaa acgccaaaag aatatatggt taccattaac
     1081 catattgaca atcaaacagg tcaagtgatt gctacagatc aagcaaaagt gacttctggt
     1141 tctacttaca ctgctaaggc aaaagcattt aaatatagcg aaggtgctaa agataatttt
     1201 agctacaaag tgattggtaa tgatactcaa agcaaaacaa tcacaggtaa tacaacaatc
     1261 acattcaatt ataatcaagt gcatcaaatc aatttgttgt gcaataacaa ctacaaaaat
     1321 gaggggcgag ttgatatttt gaaagaacag ataatcaacg tcactcacgg tgaaagtgtc
     1381 actttaactg ctccttctat tcaaggatat gttttagacg atcgggtaga accaacaaat
     1441 tcagtaacct taaacaatgt gactgaaagt caaaattata ttttcaacta cactcgtaaa
     1501 tttaatgtga ctgttaatca tgtaaatgct gataccaacg ctgttctctc tagtgaatca
     1561 aaaacagtat acgaagggga tagcttctct actccttgga aaaatatgac tgatcaaaat
     1621 tatttcttgt gcagaaatga cgatcctacc gttaccgtgg ataaaaacgg ccaacgatcc
     1681 attaataacg tcgatcaaaa taaaacaatc acattcaaat ataaaaagat ttcattagac
     1741 gaattacgta actttatgcg tcaaaaagaa cttgcttggt taaatgatta tcgccaacaa
     1801 aatggtgttg ccccaatgca attcaatgat attgttcaac aggctgcaga tattcgagct
     1861 aaagaattac aagtcagctt cagtcactat cgccctggtg gtggtacttt ccaagattta
     1921 ttggaaagct taggctgcta tggtgctaag ggagaaaaca taagtcaaac tgggctgtat
     1981 attgatgacc ttttatcaga cgatggtgct atcgatgcca tggtaggatg gaaaaatagc
     2041 cctgcccata atgcaaattt actttataat aaccaaagta ttctcggatt aggtcataat
     2101 tttgtagttg actctgctgg tagaattggc gtagataatg tatttattag tgcgaatcca
     2161 atcttcaaat aaaagaaaac aaggctgaaa atcagccttg tttttatatt tatttgagtt
     2221 ttaaaactac atagcccaat g
//
LOCUS       DAHUWM010000280         2232 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHUWM010000280 DAHUWM010000000
VERSION     DAHUWM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.286"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..544
                     /locus_tag="NK788_002700"
     CDS             <1..544
                     /locus_tag="NK788_002700"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6770990.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="NK788_002701"
     CDS             complement(900..1472)
                     /locus_tag="NK788_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCH6770991.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="NK788_002702"
     CDS             complement(1488..2093)
                     /locus_tag="NK788_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCH6770992.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DAHUWM010000281         2191 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHUWM010000281 DAHUWM010000000
VERSION     DAHUWM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.287"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..2101
                     /gene="acm"
                     /locus_tag="NK788_002703"
     CDS             <1..2101
                     /gene="acm"
                     /locus_tag="NK788_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCH6770993.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     LIVTICAGIFLQKYKKV"
BASE COUNT          865 a          349 c          422 g          555 t
ORIGIN      
        1 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
       61 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      121 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      181 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      241 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      301 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      361 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      421 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      481 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      541 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      601 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      661 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      721 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      781 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
      841 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
      901 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
      961 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1021 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1081 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1141 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1201 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1261 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1321 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1381 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1441 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1501 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1561 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1621 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1681 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1741 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1801 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     1861 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     1921 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     1981 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2041 tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta
     2101 gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt
     2161 ataatcgaaa aaagaagcat ttttatatat g
//
LOCUS       DAHUWM010000282         2178 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHUWM010000282 DAHUWM010000000
VERSION     DAHUWM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.288"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(205..1104)
                     /locus_tag="NK788_002704"
     CDS             complement(205..1104)
                     /locus_tag="NK788_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCH6770994.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="NK788_002705"
     CDS             complement(1114..1470)
                     /locus_tag="NK788_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCH6770995.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="NK788_002706"
     CDS             1825..2121
                     /locus_tag="NK788_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCH6770996.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          680 a          418 c          369 g          711 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacat
//
LOCUS       DAHUWM010000283         2173 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHUWM010000283 DAHUWM010000000
VERSION     DAHUWM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.289"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(284..1624)
                     /gene="glnA"
                     /locus_tag="NK788_002707"
     CDS             complement(284..1624)
                     /gene="glnA"
                     /locus_tag="NK788_002707"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCH6770997.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1652..2044)
                     /locus_tag="NK788_002708"
     CDS             complement(1652..2044)
                     /locus_tag="NK788_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCH6770998.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          620 a          489 c          372 g          692 t
ORIGIN      
        1 cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt
       61 tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt acttgttgca
      121 gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
      181 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt agtttctcta atactcaggt
      241 caaaacgctg atttcacagc ctctaattca cggtattcct gttttaataa agttctagat
      301 attgttctct ttcccactcg gaaacagttt gtctgaatgc cgcccattcg agacgttttg
      361 cttcaacaaa atttgagaag atatgttgtc ctaatgcgtc aatcattaca ttatccttac
      421 gcaattcttt gatagcatta tggatagttg aaggtaagtc ctggatatgt gcagcttctc
      481 tttcttcctc atccattaca taaatgttac ggtcaacagc tgctggcggt gtgatctgat
      541 tacggatacc gtctagtcct gcttgcaaca atactgccat tgccaagtat gggtttgcag
      601 atggatcaac tgaccgcaat tccaaacgtg tggataaacc gcgagattct ggtacacgta
      661 ctaatggcga acgattacgg ccactccatg cgacataaac aggcgcttca taacctggaa
      721 cgagacgttt ataagaattg actgttggat tgcaaacggc tgtgtaggca cgagcatgtt
      781 tcagcaaacc acctaagaaa tgataagctg tttcacttag ttccaattca ccgtctttat
      841 catagaaaac atttccttca tcattgaaca aagacatatt gcaatgcata ccagaaccat
      901 tgatcccaaa taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg
      961 ttttgacaac gagcttgaag gtttggatat tatcacaagc atctaccaca tctgcatatt
     1021 taaagtcaat ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc acttcaaagc
     1081 ctagactttc taattccaac acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg
     1141 caaaatcaaa ataaccacct ttatcattca aggtcgttgt gatcccaccg tcttcgtcta
     1201 atttaaataa gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc
     1261 gcatatcttt caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat
     1321 ccggattata gatatcacaa attaaacgag caacttttcc atgagcactt tcccacggga
     1381 agatcatcca agtggagaga tctggataca aatacatatc gctttcttca attcgaacaa
     1441 atccttcaat cgaagaaccg tcaaacatca ttttattttc taatactttg tcgatctggc
     1501 tcactggaac ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa
     1561 atcgtacgtt ttcttcctca atgattcgtt tgatttcttc acctgttatc tgtttcttta
     1621 ccatggtatc cctcttcctt ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt
     1681 gcgactgata atgacctgtt ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa
     1741 tttgtcgaac gtcatgatct gtcaacggct tcttgttttt tgcttcttgt tttttcgcct
     1801 gtgccatttc gtatacacgt ttgatcccag ccatattcaa tccctctgaa agatagtctt
     1861 taatctcaag caacatatca atatcattca gcgaatacat acgtcggttt ccttcgcttc
     1921 gttccggatg aactaaatct tgttcttcat aatagcgaat ttgccgagca gacaaatcag
     1981 tcagcttcat aactgtacca attggaaata cggacataga tcggcgcaat tccttttctc
     2041 ccatcagacg tcccctttcg ttttataagt tggctaaagt gtaccaatta aaaaaaatat
     2101 tgtcaactgc atatgttagg ttttctaaca tgagatctct attttttata aaataacaaa
     2161 aaaataagaa cac
//
LOCUS       DAHUWM010000284         2166 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHUWM010000284 DAHUWM010000000
VERSION     DAHUWM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.290"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            167..796
                     /locus_tag="NK788_002709"
     CDS             167..796
                     /locus_tag="NK788_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6770999.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(920..2029)
                     /gene="ugpC"
                     /locus_tag="NK788_002710"
     CDS             complement(920..2029)
                     /gene="ugpC"
                     /locus_tag="NK788_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCH6771000.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT          615 a          402 c          404 g          745 t
ORIGIN      
        1 tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg atactcacac
       61 tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta agtgagttat
      121 actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga gaaaaaattc
      181 agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta tcccaatggt
      241 catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg tgccattatt
      301 tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta cgacatttgg
      361 tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc
      421 gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa atggacagtt
      481 tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga ttcattcttt
      541 agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg aaacatatta
      601 ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc ttatccacag
      661 tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat attttgatat
      721 ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa
      781 gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt aatggataat
      841 aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat cttctggcac
      901 agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct ttatcaaaga
      961 agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta tggaaatctc
     1021 ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc atcgtttcag
     1081 ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt gaagcttcaa
     1141 gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact tcttttcctt
     1201 cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc ttaagcccat
     1261 gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg gcaggagatc
     1321 caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc ggtgtaccga
     1381 tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc attgcttctg
     1441 tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt ttggcaatct
     1501 cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca tccattaaga
     1561 agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt tgtccaccag
     1621 ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct gctgcattgt
     1681 ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca aaagccatat
     1741 tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg gcaatatcac
     1801 ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct ccttcagaga
     1861 tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg gaaggaccaa
     1921 caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca gaataatttt
     1981 ctgcattgtc atatttttta tatacgtttt tcagagccat ttctaccata atacgttcca
     2041 cactttcttt tttagtttta ttaactattt aaagtataaa tgaaagcgtt aactaacttc
     2101 aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt ttaagcttta
     2161 ataaat
//
LOCUS       DAHUWM010000285         2165 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHUWM010000285 DAHUWM010000000
VERSION     DAHUWM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.291"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..593)
                     /locus_tag="NK788_002711"
     CDS             complement(<1..593)
                     /locus_tag="NK788_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCH6771001.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIA"
     gene            complement(604..1272)
                     /locus_tag="NK788_002712"
     CDS             complement(604..1272)
                     /locus_tag="NK788_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCH6771002.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1402..1986)
                     /locus_tag="NK788_002713"
     CDS             complement(1402..1986)
                     /locus_tag="NK788_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCH6771003.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          768 a          363 c          291 g          743 t
ORIGIN      
        1 tcagctataa caatagtaaa tcctttattt tttaaatcac aaagtaagtc agcaagtttc
       61 ttagtcgctt tgaagtccaa atttgaagac ggttcatcca atataattaa tttagagttt
      121 agcgataaac tacatccaat agaaattttc tgccgttcac cacttgatag cgaaaaaatg
      181 tttttatcta atatatttga aagatctaat aattcagcta attcattgat tcttttatcc
      241 atatgctctt ttgtaaatcc aaagttttcc atcgaaaata ctaactcact cgttgtattt
      301 gttgtaaaga attgtcctct aggatcttga cttacgtagc caatattttt tgatatactt
      361 tcttgtgtta aggattgtag cgagttattt tcaaataaaa tttccccaac caattttccg
      421 tcataaagtc ttggaataag tccattcatt aactttaata gtgtggattt cccactccca
      481 gaagaaccag taatcaatgt aagactgcct ttttttattg aaaaattaag gtcaacaagc
      541 gcttgttcct taacatcctt atatgaaaaa gaaacattgt taaattcaaa caattctttc
      601 acctcaaatc aaaatactta agaaaaaaat agtaacggaa aacaggatga tatagtctaa
      661 taagctcatg tttgttttat agtaagaact tcttttcaca ggagattcta tcccctttgt
      721 tattgcatta gcggtcattt cttcagcaat catagttgca cgtataagaa cgggaactaa
      781 cagaaattcc aaatattgca gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa
      841 agattcttta atcattctta tttcagaacg ataagtcgga aagaatctga tcactactgt
      901 aatacttaac attatttttt tcggtaaatg tattttttct aaagaggata ataattcatt
      961 gctagtatag acactaacca acaaccatga gatcattacg accggcaaca ttcgaaatac
     1021 aatatataag gatgtcgcaa aaaatatagt aaaaaactct atcttgatag catcaataat
     1081 tttcattact aaaaacagaa ctgaataaat caagatgaaa taaatacatg tctttatttt
     1141 acgaaacatc aatactaaaa ctgtagcaat aatgaaacac cccacttgaa atatatttcc
     1201 ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt
     1261 gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa
     1321 aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt
     1381 ctcaatgcaa gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg
     1441 atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc
     1501 atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa
     1561 attttgattg atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt
     1621 cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa
     1681 aatcaaatcg aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt
     1741 gaagttctct ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt
     1801 aaactcttca ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa
     1861 taacgcttcc ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcag
     1921 gcacagttgc cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact
     1981 ttctattaac ttttggtgag ttataatata atctctggtt tctatgggta cttcctccta
     2041 ataagatgaa ttaactttat tatcttacac aggacttgaa gtttcaatcg ttaacgttta
     2101 ttactgaact cattccttag agctaaacta atgagaaaga gcagaataac tgatacaaaa
     2161 agcaa
//
LOCUS       DAHUWM010000286         2161 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHUWM010000286 DAHUWM010000000
VERSION     DAHUWM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.292"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..474)
                     /locus_tag="NK788_002714"
     CDS             complement(<1..474)
                     /locus_tag="NK788_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771004.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKA"
     gene            complement(446..1645)
                     /locus_tag="NK788_002715"
     CDS             complement(446..1645)
                     /locus_tag="NK788_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCH6771005.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1775..>2161)
                     /locus_tag="NK788_002716"
     CDS             complement(1775..>2161)
                     /locus_tag="NK788_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCH6771006.1"
                     /translation="AVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLG
                     NNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSL
                     VLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          681 a          433 c          370 g          677 t
ORIGIN      
        1 agctttgatg tgggtagaat ccagatacaa attatcctct gttacaaaac ctgctttaac
       61 tgcttgttct aaaatatagg caaaaatatc ctcaaacacc gtcgtttctc taaatcggcg
      121 aacataattc tttccaaaag ttgagaaatg ggggattttc tcctcgaagg agtagcctaa
      181 gaaccagcgg taagcgacat tggtatcgac ttccttaatg gtttgtcgca tggaacggat
      241 accgaataaa tactgaataa acaccaattt gatcagaatc actggatcga tacttgggcg
      301 acctaaatcg gagtaagtgg cttcgacgat tggataaata aattcaaaat caagcgcggc
      361 atcaatcttt cgaacgagat gatcttgagg aactagatca tcaatcgtaa gcatcgcaaa
      421 ttgatttcta ccattcgtat tgtttttaga catcatagct atcactcctt gcattcgttt
      481 tcataaatct attataaaag aaaagactgt agacatcatc cataaaatgg actttgtcta
      541 cagtctgcgc attctcatta gagaacactc ccttttttgt gacttttctg aagctagagt
      601 gaatactttc aactgcatta gtagtgtaca tgatttttct aatggcgctg ccataatcaa
      661 agagttgttc aacatgagaa aagtttcgtt tgcatacatc tactgcacca gaataatgag
      721 accaccgatt ttgaaaactg tcgaaggcag cgtgtgcagc gtttaaagaa gaagcactgt
      781 agaatttttt catgtctcga cagacttcct tataatcctt acttgggagg tatcgtaacg
      841 catttcgaac aagatgaaca atacagcgct gcacaatgac agaaggaaaa attgcttttg
      901 cgccttcttt gagaccggaa acaccgtcca tcgaaatgaa aaaaacatct tctacaccac
      961 ctactttcag ttcatcaaat acttgcatcc agcgattttt agattctgtt tgattgagcc
     1021 ataggcctaa aatctccttg tttcctttaa gatcatagcc aagaatggtg tacacagcat
     1081 attctttagc ttcatagttt tcgcgtaaag tgacgtacat acaatcaacg aatagaaatg
     1141 cataacactt ggctaatggc cgagcttgcc attcttccaa ttcaggaagg acggcatccg
     1201 tgatatcaga aatcatttcg tgggaaatat caaagccgta aatatcttca acggttgcgg
     1261 cgatatcgca ttgactcatt cctcgtgcat acatggaaag aaccttacct tcgatgtcgg
     1321 agacatctcg ttttctatta ggaatcaact caggttcaaa agaagcctcc cggtctctgg
     1381 gaacatcaat gtttacttta ccaaagctcg ttttaagcgt cttattgcca taaccgtttc
     1441 gtcgattgtc atgtttcttt ggctctttag aatgggcgtc ataccctaaa tggttattca
     1501 attctccttg aagcattttt tcaaataagg gaccaaacac atcttttaaa acatcttgca
     1561 tatcctctac agattcgggt tgataagtat tcaaaatgga ttcagctaac ttctcagcat
     1621 caggatttct tttctttcta gccatcgtgt aatcacctct tgatcttatt ttagaaaaat
     1681 aaaaaccgtg aagcaaccac ccgcctagga ttgatggtta cttacacggt ttatattaca
     1741 ctctcatttt tggtgctaaa aagcaccgaa aattttaatt attgtcaatt aataattctt
     1801 ttggattcct tcttaatata ttgaatgaag aaataatcaa tgatacaaga agtactaaac
     1861 tcataaatat aactacataa atcatgaatt taggtaatac attgatatct aaacttgata
     1921 atgtcttgtt aaatccttca gcttctgccc caccacctaa ttggctagat gcagattgtt
     1981 tagctatttg tttagctata tcaccagtaa ccttattcaa tatattattc ccaagcttgt
     2041 cagctgtata ttgagctaag aaataggaac caacgaatgc ggggattgat ataaatacca
     2101 tctcaattag gaattgacca aaaatttcta attttgatat acctaatgat agtaatactg
     2161 c
//
LOCUS       DAHUWM010000287         2157 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHUWM010000287 DAHUWM010000000
VERSION     DAHUWM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.293"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..914)
                     /locus_tag="NK788_002717"
     CDS             complement(<1..914)
                     /locus_tag="NK788_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCH6771007.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="NK788_002718"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="NK788_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCH6771008.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..>2157
                     /locus_tag="NK788_002719"
     CDS             2006..>2157
                     /locus_tag="NK788_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771009.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEA"
BASE COUNT          731 a          381 c          417 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaa
//
LOCUS       DAHUWM010000288         2144 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHUWM010000288 DAHUWM010000000
VERSION     DAHUWM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.294"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            58..498
                     /locus_tag="NK788_002720"
     CDS             58..498
                     /locus_tag="NK788_002720"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCH6771010.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            626..1309
                     /locus_tag="NK788_002721"
     CDS             626..1309
                     /locus_tag="NK788_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCH6771011.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1618..1691
                     /locus_tag="NK788_002722"
     tRNA            1618..1691
                     /locus_tag="NK788_002722"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1652..1654,aa:Arg,seq:tct)
     gene            1850..>2144
                     /locus_tag="NK788_002723"
     CDS             1850..>2144
                     /locus_tag="NK788_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCH6771012.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTS"
BASE COUNT          712 a          335 c          436 g          661 t
ORIGIN      
        1 acttttataa gttctataac gttgattata tttttaattc ttattcttct cgtcgtcttg
       61 gttccccgaa tttttggttt gactccttat attattgagg acaaagaaat gagtccatat
      121 tatgcacaag gaaatctgat atatgtacga ccgaaaaata caaatgagat tttagtcgga
      181 gatattatta catattatga aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc
      241 gttgaaaaca accacgaaga tttttataca aaagctgata caaagactta tattgagcct
      301 ggagtagtaa aaaagagaaa tattatcggt agtcccatct ttcagattcc atatattgga
      361 ctagtccttt caaaaacggc atttgcatgg attcaggggt tattctttac gattgctttt
      421 tgcctaacag gaatcacagc atggaatacc tttgtagagt tgaaaaagaa gcggagacga
      481 acacagaaat ttttgtaaga atgttaaaaa cataaatgta ttccagagat ccaacgactc
      541 ttctgaacat gaaacacgag aaaacactca tgcagcttgt agatttagtc agttgttcgt
      601 ataatgtgtg aagaggagga gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa
      661 agaagaacta ttggatttta tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa
      721 actggaagaa gaagctcatg tagcagaagt accagttatt ccccatgaaa cagttgtatt
      781 tttgaaattt ttacttggac agcttcagcc tgaacgaatc ttggagatcg gagcagctat
      841 cggtttttca tcaagtgtca tggccacgac aatcggagaa aacgctcatg ttacgacaat
      901 tgatcgtttc gatgtcatga tcgagaaagc taaaaaaaac tatgaacgtc taggactgac
      961 agataaagtg actttgcttg aaggtcaagc agcagatatc ttgccggagt tgtctggtcc
     1021 gtatgacttt atttttatgg atagtgcaaa atcaaaatat attgaatttt tacctgaatg
     1081 cttacgtttg ttaagaaaag gtggcgtttt gatggtagat gacatcttcc aaggaggaac
     1141 gatcttgctc ccagatgaag agattccccg tggcaaacga gctatccatc gtaaattaaa
     1201 tgaatttctt cgtgttgtga tggatcatcc cgatttgaca tcgacaatct tgccattagg
     1261 tgatggtgtg atcttaatga caaaggaatc agaaacaatc gagttgtgat acatgattat
     1321 ggcagcctgt agataatcga ttgaatcgag ggctcaattc gaacttgatg taaaagtttc
     1381 tatatttgta agtcaattaa aggatacagg aaatctaaat aaacaggagt agcatgaagt
     1441 caatgtagct cctgttttca attgataacc ttagaacagc tgaaggagaa tgtctcgttt
     1501 taaaagattg atttagtagt tgggccaaaa taattcatta tttgctaatt aaataactat
     1561 ttcgtattga cagaagcaat ttaattatct ataattattg atgttccgct gattcttgtc
     1621 atagtagctc agctggatag agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc
     1681 ctcctgtgac gctaaaaaac cgttagaaac tgaggtttct aacggttttt ctttgttttt
     1741 tgtgccaata ttgtttgttt tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac
     1801 aattataagg agcaaaaaaa taaaactcat cagaaaagag gaagaagata tgacgatact
     1861 atctagaatt ttagtgactc tagttgcaat tgaatttttt tatatcatgt atatcgaaac
     1921 ggtgagaact gattctgaca cgacaagtag ggtttttaaa atgagtaaag aagagttaag
     1981 cagaaaatct gttcaaacat tgtttaagaa ccaaggagtg tacaatggac tattaggagt
     2041 gggactactt tatggagctt atctttcttc cgcatctaaa gaaatcacaa gtatgctatt
     2101 aatatctatt ttttttgttg cattatatgg cagtttgact agcg
//
LOCUS       DAHUWM010000289         2134 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHUWM010000289 DAHUWM010000000
VERSION     DAHUWM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.295"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            32..>2134
                     /locus_tag="NK788_002724"
     CDS             32..>2134
                     /locus_tag="NK788_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCH6771013.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLE"
BASE COUNT          799 a          292 c          353 g          690 t
ORIGIN      
        1 catattaatt aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa
       61 ttttagaaat tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg
      121 tgagaatgat tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag
      181 ttattactat agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg
      241 gtggcaaggg cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt
      301 tgacaatgag atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga
      361 attgatttca aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa
      421 agctattaga aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga
      481 atttagaaat tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct
      541 aaatttctgt atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc
      601 tagtaatcct gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat
      661 tttcaagcat atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa
      721 aaacaatcga aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga
      781 taatgttgag tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc
      841 tgaaattagg aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt
      901 gtattctcct aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa
      961 atttctttca atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct
     1021 aaatatgatt tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact
     1081 acttaatatt atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac
     1141 tctgttcgaa ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc
     1201 tgtacattta gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg
     1261 caaatctatc gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa
     1321 aaagaacaat ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg
     1381 attgcttttt tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc
     1441 tcaaatagta aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa
     1501 tgttggtagt acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag
     1561 aaagtgtgct attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta
     1621 taaaagcact gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa
     1681 tgaaaacaat tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga
     1741 tgtgaataaa gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga
     1801 taaaaaaaca ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa
     1861 tttagcgatg ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg
     1921 tgaaaaaatt gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc
     1981 atgtcaggaa atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt
     2041 gtacgaggaa actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc
     2101 tgatttgaat atagcaataa tttctttatt agag
//
LOCUS       DAHUWM010000290         2129 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHUWM010000290 DAHUWM010000000
VERSION     DAHUWM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.296"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            743..1075
                     /locus_tag="NK788_002725"
     CDS             743..1075
                     /locus_tag="NK788_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCH6771014.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1694..2035
                     /locus_tag="NK788_002726"
     CDS             1694..2035
                     /locus_tag="NK788_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCH6771015.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          753 a          281 c          437 g          658 t
ORIGIN      
        1 tattacaaaa taatagctat tttaatagaa tttataagtt aaaatattat gataaaaatc
       61 acaaacgtag ccttttacgt cataagtgtt aaaagaaata attaatttca aatatagaaa
      121 agggaacgta tgtttgcata ctcctttttt ttgagttata ataatacgcg aagagagata
      181 atttcattat ttaaacaagc taaaaatctt gatattactt cttttcaggc aatacgaata
      241 ttattcgctt acattttttg tataaattta attgtctgct atgcttgtga tgaattgatt
      301 ttcgttctgc acttttggat gataaatttg gacaattgaa aatacaaaaa aaagaaaggc
      361 ggtatgaact cggaaggctg gtaaccttcg tcagcgttca tacacggcat taagatttgg
      421 cccttaatgc gttaaccttt cagtgttacg gtattacccg caatggattt agtataacag
      481 atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac
      541 gatgaatcta tgcctaagca caatgttata aaaatgtccg tgtggctctt tgccgtatga
      601 cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat
      661 gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta
      721 aagaaattgg gtggtttatt ttatgtcata tacaggaatt ttgagtctca aagatatttg
      781 tcactacggt aaacggtgta ccgctacaga gaaaatcacg aagaaactat caactggcca
      841 aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagagga
      901 aatgatttat tacattggga aacaaaagca aataatttta aaagatccta tccccttaaa
      961 agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag
     1021 aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg gctgaattga
     1081 tcattctcat aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc
     1141 taaaggaact tagagtcact agaaaaataa ggagtagact tgatctatca acaattccct
     1201 tcggggagtg acggggtagg ttttaacgat tggtaacgta aaaaattggt caatcgagta
     1261 aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa ataataagcg cagagtaaac
     1321 aagagggaat ttagaagtta gacaagttag aatagcgtct agattaatca tgagcagcta
     1381 ctaagggatc tagactttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg
     1441 ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt
     1501 tgattattgg atcaatcgag catttaccgc tgtctactag ggtttctcgt ttcggacgcc
     1561 ataaacgagt cgaaattttt tatgtatttt taagtaaacg aaaaacgata aaatatgaac
     1621 aagtcctgaa taaagtgaaa aaattgatat aaaacataga gaaaatgaaa tttacgtaat
     1681 ggaggaatga caaatgaaaa attatgatcc gaatatacga ttgggcacac atactattaa
     1741 ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaagag gcggaaattg
     1801 taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa cattgactga
     1861 taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat ggtttaaaat
     1921 gacgcttatg aatgataatg gagacgaatt atctgttgaa gatacttggt catatttgag
     1981 tgattatatt gtcagctttg aaatcattga atttgtagcg gataaagaag aataaaaact
     2041 ggaggaataa attttacaga taaaggctta gaaggagtca cgatgattaa taaaaaggta
     2101 gtattacctt aggcattgag taaggaatt
//
LOCUS       DAHUWM010000291         2129 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHUWM010000291 DAHUWM010000000
VERSION     DAHUWM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.297"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(112..345)
                     /locus_tag="NK788_002727"
     CDS             complement(112..345)
                     /locus_tag="NK788_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCH6771016.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(360..1310)
                     /locus_tag="NK788_002728"
     CDS             complement(360..1310)
                     /locus_tag="NK788_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCH6771017.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1307..>2129)
                     /locus_tag="NK788_002729"
     CDS             complement(1307..>2129)
                     /locus_tag="NK788_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCH6771018.1"
                     /translation="GLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLMGDAISHA
                     VLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSSFLALGVI
                     LIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMA
                     KAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFI
                     SATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT          712 a          401 c          256 g          760 t
ORIGIN      
        1 ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta
       61 tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa
      121 accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc
      181 taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg gactaacact
      241 tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat
      301 tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat
      361 tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta
      421 tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata
      481 ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc tacgaataag
      541 gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct
      601 tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa
      661 tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat
      721 ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct
      781 ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg
      841 taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc tttctcactc
      901 tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc
      961 tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt ttccaagtta
     1021 acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt
     1081 tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt
     1141 gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt taatttttcg
     1201 ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa tataaaaaga
     1261 gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat aacgatctct
     1321 ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg
     1381 ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca ataaacaatc
     1441 caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt ttcaagctat
     1501 ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt aatatagttc
     1561 ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat agataatgat
     1621 aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa gtgattaata
     1681 attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca attgtaatcc
     1741 acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat aaatccgttg
     1801 agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg aaagtaatac
     1861 caattgctgt atctcctttt attacactat tgctttttat aaaagtaata atgatagaag
     1921 ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg
     1981 ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa
     2041 taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat
     2101 tctgtaaaaa gtgaaagtca tacagacca
//
LOCUS       DAHUWM010000292         2121 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHUWM010000292 DAHUWM010000000
VERSION     DAHUWM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.298"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(548..913)
                     /locus_tag="NK788_002730"
     CDS             complement(548..913)
                     /locus_tag="NK788_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771019.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(989..1234)
                     /locus_tag="NK788_002731"
     CDS             complement(989..1234)
                     /locus_tag="NK788_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6771020.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1304..1558)
                     /locus_tag="NK788_002732"
     CDS             complement(1304..1558)
                     /locus_tag="NK788_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771021.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1722..2045)
                     /locus_tag="NK788_002733"
     CDS             complement(1722..2045)
                     /locus_tag="NK788_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771022.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          799 a          362 c          371 g          589 t
ORIGIN      
        1 aacaaaaaaa gaaagcgtta ttattttagt ctattttcag ggggggaacc ttatgataaa
       61 aaaactattt ttactatcaa tattaacgct agcaatggca ggttgttctg actctggctt
      121 agcaactgaa ctgatcacaa aatcaactga agccacgaca gaggagttaa aatgtggaaa
      181 cgatcacagt gactacagag gcttaaatcg acgatgcatg ggaatattag gcagaagcaa
      241 ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag
      301 aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca gacgtcgaag atgcggtact
      361 tttaaaccac gtcaggagat gttcatttat gtctagggaa gttggcgatg aaccaaaact
      421 agagtacgct tttagttttg cacgtgagga tgaagataac cctaaaactt acgacatttt
      481 agcttgatct aagatggtta aagcttttta atgaagtaag aggagataga aggcttttct
      541 agttatatta ggtaagataa attccttttt gctccaaatt tgctaaacat tctgctagat
      601 aaacttcatc gtgttccgga taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa
      661 atgcttcgca tgatttgcca cgatacatag gatttttcca ctctggtgct gggctgtaac
      721 cacgtttttc catttcatcc atgacaagct gatgatattg gaataatcta taaggagaat
      781 agtgaaaaac ataatccaca gtagcatgtt tctttcccca gccatttcct cttaatgcac
      841 agcattcccg atgttgtcct aaaagctgtt gacgaggaag ctttgaaatc aatttttcat
      901 gccataatcg catagaaatg tctcctttat atatagattg ttaaccttag acgttaaatg
      961 aaaaaaccag caccaaggct ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac
     1021 caattaggta atcaacggaa atatcgtaaa agtcagcgat atagattaat tcaatgatgc
     1081 ttggaatttc ttctccttct tcgtacttac gatatttgaa taaaggaata cttaacgcgc
     1141 tagaaacagc tacttgagat aaagatcggc cagttcttaa gaaattcaga cgacaacgta
     1201 aagtatccag taaaatagct tcttcgatac tcatttaatc acctttcatt atagtagaat
     1261 agttataatg actatagaaa gctacacatg caatcaatag tactcaatgt tttttattta
     1321 aacgtatcaa ataaagaatg acgatcaata aagacaaaac cagtcctgat aattcttcta
     1381 taaaaacact atcgggttca aagtagttgt aattaaaaac tttccacata aagtagatag
     1441 ccggaagagt caaaatagct aaataaaacc gttctttgtt ccaattcata agataaacaa
     1501 tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt
     1561 aacggactcc tcaacgattc atataataaa agataacatg ttattgcata catcacaaat
     1621 gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa atgaaaaagc agaacagttt
     1681 tctgctgata agcaggaact gttccgctga atgtgtctta tctatcgaga aaaaatcaat
     1741 ggattgatat ctaagtaatt caatacatat aaaaagagga cgatcgcatt cactaaaaag
     1801 gaaagaatga tgatcccata gcgaatggaa acatatttaa caaatacttt tgtttgaata
     1861 cgggcatatc tttttccttt atagctgagc atcatgacaa gaagcaaaag ggtaatcaca
     1921 ccttgaaaaa tcattccaat aaacagaatt cctgaggttt gataatttaa ttggagaaaa
     1981 taagctgctg ccgcaactaa atcgaataaa atcgcaataa atggcaaaat aatcactcct
     2041 aacattgaga ataaataaaa gaataacatt ttttctaaaa taagcaaata aaaattaaag
     2101 taacaaaata gttatgacac a
//
LOCUS       DAHUWM010000293         2103 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHUWM010000293 DAHUWM010000000
VERSION     DAHUWM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.299"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            169..1539
                     /locus_tag="NK788_002734"
     CDS             169..1539
                     /locus_tag="NK788_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCH6771023.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="NK788_002735"
     CDS             1560..1844
                     /locus_tag="NK788_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771024.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cat
//
LOCUS       DAHUWM010000294         2086 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHUWM010000294 DAHUWM010000000
VERSION     DAHUWM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.300"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..177
                     /locus_tag="NK788_002736"
     CDS             <1..177
                     /locus_tag="NK788_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771025.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="NK788_002737"
     CDS             190..690
                     /locus_tag="NK788_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCH6771026.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2086
                     /locus_tag="NK788_002738"
     CDS             787..>2086
                     /locus_tag="NK788_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCH6771027.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSI"
BASE COUNT          679 a          284 c          465 g          658 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatac
//
LOCUS       DAHUWM010000295         2078 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHUWM010000295 DAHUWM010000000
VERSION     DAHUWM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.301"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            269..2074
                     /gene="glmS"
                     /locus_tag="NK788_002739"
     CDS             269..2074
                     /gene="glmS"
                     /locus_tag="NK788_002739"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCH6771028.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT          675 a          394 c          441 g          568 t
ORIGIN      
        1 tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga taaaaaacaa
       61 aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga
      121 gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac
      181 aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga
      241 attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa
      301 aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc
      361 agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat
      421 tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac
      481 acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag
      541 tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac ttaaaattgc
      601 ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat
      661 tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat
      721 caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc
      781 taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga
      841 tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt
      901 taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc
      961 ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc ctttttatat
     1021 gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac
     1081 agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg
     1141 aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagacttt
     1201 ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg gctataacac
     1261 acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga
     1321 cagccgccaa gtgctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa
     1381 tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc
     1441 cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt
     1501 agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca
     1561 tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga
     1621 agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggtgc
     1681 tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc
     1741 agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac
     1801 accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa
     1861 agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga
     1921 aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt
     1981 accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc
     2041 acgtaattta gcgaaatcag tgacagtaga ataataaa
//
LOCUS       DAHUWM010000296         2074 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHUWM010000296 DAHUWM010000000
VERSION     DAHUWM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.302"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..219)
                     /locus_tag="NK788_002740"
     CDS             complement(<1..219)
                     /locus_tag="NK788_002740"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771029.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="NK788_002741"
     CDS             complement(330..584)
                     /locus_tag="NK788_002741"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771030.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="NK788_002742"
     CDS             complement(777..1052)
                     /locus_tag="NK788_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCH6771031.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="NK788_002743"
     CDS             complement(1024..1977)
                     /locus_tag="NK788_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCH6771032.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          634 a          377 c          299 g          764 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcag
//
LOCUS       DAHUWM010000297         2038 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHUWM010000297 DAHUWM010000000
VERSION     DAHUWM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.303"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..442
                     /locus_tag="NK788_002744"
     CDS             <1..442
                     /locus_tag="NK788_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCH6771033.1"
                     /translation="HRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVMALTIAKPA
                     DCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFGGVSKKSES
                     KQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            456..1205
                     /locus_tag="NK788_002745"
     CDS             456..1205
                     /locus_tag="NK788_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCH6771034.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1205..2035
                     /locus_tag="NK788_002746"
     CDS             1205..2035
                     /locus_tag="NK788_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCH6771035.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT          647 a          282 c          403 g          706 t
ORIGIN      
        1 ccacagatta cttcatggac aagtaatatt ttcttggact aaacagtatg atattaacca
       61 tattattgtg gctgataaca aagttccagg agatccaatg tctgtgatgg ctttgacaat
      121 tgctaaacct gctgattgca aattagacat tgttaaaatt agtgatgtta aagggattat
      181 ggaaacatcc ccaaatgata attacatgat tttaattaaa ggacctaagg aagcttttga
      241 attagttcaa aatttaccag aaattaaaga aattaatttt ggcggtgtgt ctaagaaaag
      301 cgagtccaaa caatatggaa aggcagtctt tttaactcct gaagaattag aagccacaaa
      361 aaaaattctt gatcaagaag tgaaaatatt tgtgcaacaa gtcccatctt caagtattga
      421 aaatgtagat tttagtaagt aaggggggaa aataaatgtt attaaaagcc attttattag
      481 gtttagttgg tgtggtagct gttattgact ccaggttaat tggtcgacaa aatattggta
      541 gaccactaat tttgagtaca cttgccggac ttgtgctagg tgacttgcat acagggattg
      601 ttcttggggc atctttagaa ttaatttcta tgggattcgt ctcaattggg gcggctggac
      661 ctcctaatat gcaacttggt agtattattg ccactgcttt tgcaatttta actaatagta
      721 gtacagaaac agccttaaca atagctatcc cagtggctgt agctggtgaa tttatgagta
      781 ttatcatgcg catgataatt gctcaatttg gacatgctgc agataaagcc attgaaaata
      841 ataaatttag aaaagctcaa attattcata tttattggtc atttattttt aatgcttttg
      901 tttactttat tccaatcttt ttaacagtat attttggtgc tgacgttgtg gcagatttag
      961 tagataaaat tccagaaatg attacagacg ccttgactgt ctctggaaat atgctttccg
     1021 cattagggtt tgcaatgcta ttaagcacaa tgttgagtaa aaaattatat ccttacttta
     1081 tttttggatt ctttattgtt gcctattcag gtctttcact aattggcgtg acaatttttg
     1141 cagctctgat tgcctttgta atggatcaag ttaaatatgg aaaaagagaa gaggcgcatg
     1201 gttaatggaa aatagtacag aaaaaaaacg tttaatcaca aaaaaagatc ttaataagat
     1261 gtgtcttcgt tcatggacaa tcatgtgttc gtggaattat gaaagacaaa tgcatatggg
     1321 atttatgtat gggatggcgc caattttaaa taaagtttat aaaaatgatg aagagaaaaa
     1381 aagagaagct tatactcgtc atatggcatt ttttaactgt acgcctcaaa tgacttcatt
     1441 tattatggga ctttgtgctt ctatggaaga acaaaatgct caagcagaag aaggaaactt
     1501 taatactgaa tctatctcca tgattaaaac cagtttaatg ggtccttttg ctggaattgg
     1561 tgattcattt ttccaaggta ctttaagaat tattacattt gggattggat tgtcttttgc
     1621 taagcaaggt agtattttag gaccgatttt agctgttggc ctttttgcta taccttctat
     1681 tttagctgtt ttttacggcg gtaaattagg atatgagtct ggcaataaat accttactaa
     1741 attgtatcaa gaaggtctta tggatcgagt aatgcaattt gcttctatca ttggtttggc
     1801 tgtagtaggt ggaatggtgg ctagcatggt gccaattgtc acccctttaa aattcactac
     1861 agggggaaca gaacttgttt tacaagactt acttgattcc attattcctg gattattacc
     1921 acttgtatgt actttgggaa tatataaatt gattaagaaa aatgttagta caaatatgct
     1981 cttattaggt tgtattttgg cagggtttgt tttaagtata ataggtatct tataatga
//
LOCUS       DAHUWM010000298         2020 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHUWM010000298 DAHUWM010000000
VERSION     DAHUWM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.304"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            287..694
                     /locus_tag="NK788_002747"
     CDS             287..694
                     /locus_tag="NK788_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771036.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="NK788_002748"
     CDS             708..1109
                     /locus_tag="NK788_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771037.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="NK788_002749"
     CDS             1111..1482
                     /locus_tag="NK788_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771038.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="NK788_002750"
     CDS             complement(1518..1820)
                     /locus_tag="NK788_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771039.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          764 a          270 c          337 g          649 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt
//
LOCUS       DAHUWM010000299         2016 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHUWM010000299 DAHUWM010000000
VERSION     DAHUWM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.305"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(197..631)
                     /locus_tag="NK788_002751"
     CDS             complement(197..631)
                     /locus_tag="NK788_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6771040.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            779..1920
                     /locus_tag="NK788_002752"
                     /pseudo
     CDS             779..1920
                     /locus_tag="NK788_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT          793 a          367 c          339 g          517 t
ORIGIN      
        1 ttagtcggtc aagtacttct tttttaaaaa aagtattcat cataaaagcc actacttgcg
       61 gatcttcatc ataaaattct gtagcagtac ttgaaaagat gttccaataa atatctaatg
      121 ctgcatattg aatttgtacc tcttttccag aaaaagactt tctgccatta ttagaaaagg
      181 agatagacaa accgtactat gctaaaaatg cattaatctt tttcattttc gtatacaaat
      241 aagaggcact atagtttgtt tgctgactga aactgataat cgaatttgtc tgacagctga
      301 acaaagaagt caagatctga taactgactg attcgttgca gtatcttcca aatagacgat
      361 aatagcagta agccggatca attccttcaa aattcaactt taaaaaaggt ttttgcttat
      421 caatatgcag ttctgttaga ttgagacgct caaagtcttg ttctaattct ttgatgtata
      481 gtaaaaaatt tgattcagac atatggaaat gatctgctaa atattgtgta gtaataaaga
      541 aacgatgttc agataggaaa agaaaaatat ctaattttgt attgcttttt ttcttcaaga
      601 taaacgtttt aatcgcttct tgagtttgca tgaaagaggc tcctttttac ttaaattgtt
      661 aaaacttgaa aatattactg tttttttcat aaatttagac aaaaaaacag taaacatata
      721 ggtagaatct ctactatctt atttcttgca tacaaaaaaa tcaaggagtg agagagttat
      781 gagatattca accatcttaa tatcaacatt tgcagcctta agcttatcgc ttactataaa
      841 tcagacagtc cacacagagg agacagaaac agccaaatgg acagaaacca agtgggccaa
      901 cgtcaatcga attacctttc aggatgaatt ttcatccttc aattcaggag tcgcaaaatt
      961 aagaaaagga caaacagcga ccatcaaagc aaaaattgac tattcgggag acttagcacc
     1021 tatactacaa tcagctgtta catttgagaa tgttgatccc actttgtcga ttggctatga
     1081 cgagaacact ttgactatcc aacacaagca agcgatattt accttcaccg ttacattaaa
     1141 tcaggacttg ttaaaaccag cgctttttac tgtcaaggta tcagatggac taccgaacga
     1201 tcatcattta acgccgtata gtcagcggca gacaattgag caagattcag ccatcgactc
     1261 aggaggagat cttgttgaag aaccaacaga taaaccagaa aatgaaaata agccagaagt
     1321 accgcctaca gaaaatccag acggagaaca gaaaccggaa atagaacctg gagaagaacc
     1381 cgatacagaa actcaaccag agccagacaa taaatcaaaa cctgaaataa cccctggaga
     1441 aaaaccagat gtaactccag aaccagatac ggattctgga aaccaaacag taccggaaac
     1501 aaatccagac acagataatg aaactgaaaa tccagaaaaa ccagaggtag atccagaaga
     1561 aaaaccagat gtgactccag aaccagatac ggacgcaaga gaccaaggaa taccagaaaa
     1621 aataaacaaa aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat caaatctagc
     1681 aattctaaaa ataaatgaag agcagctgaa taaaaaatca cgaatatttg atagcgccca
     1741 atcagctgaa accttaaaaa gtagcaagga tacaaccttt gctgtcctga aacgaaaaat
     1801 aaacagctac caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt
     1861 ctattaagta tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa
     1921 catttgaata atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaatgacc
     1981 ccaaaatacc agattgaaat ctgatatttg gggtcg
//
LOCUS       DAHUWM010000300         2002 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHUWM010000300 DAHUWM010000000
VERSION     DAHUWM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.306"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..378
                     /locus_tag="NK788_002753"
     CDS             <1..378
                     /locus_tag="NK788_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCH6771041.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="NK788_002754"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="NK788_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCH6771042.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2002
                     /locus_tag="NK788_002755"
     CDS             1859..>2002
                     /locus_tag="NK788_002755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771043.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DAHUWM010000301         1962 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.307, whole genome shotgun sequence.
ACCESSION   DAHUWM010000301 DAHUWM010000000
VERSION     DAHUWM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.307"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..272)
                     /locus_tag="NK788_002756"
     CDS             complement(<1..272)
                     /locus_tag="NK788_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771044.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="NK788_002757"
     CDS             complement(309..446)
                     /locus_tag="NK788_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCH6771045.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="NK788_002758"
     CDS             complement(448..894)
                     /locus_tag="NK788_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771046.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="NK788_002759"
     CDS             complement(891..1040)
                     /locus_tag="NK788_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771047.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..1386)
                     /locus_tag="NK788_002760"
                     /pseudo
     CDS             complement(1054..1386)
                     /locus_tag="NK788_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DAHUWM010000302         1954 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHUWM010000302 DAHUWM010000000
VERSION     DAHUWM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.308"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="NK788_002761"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="NK788_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCH6771048.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="NK788_002762"
     CDS             852..1040
                     /locus_tag="NK788_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCH6771049.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="NK788_002763"
     CDS             1053..1610
                     /locus_tag="NK788_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCH6771050.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="NK788_002764"
     CDS             1642..1884
                     /locus_tag="NK788_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCH6771051.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          304 c          404 g          531 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggtt
//
LOCUS       DAHUWM010000303         1911 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHUWM010000303 DAHUWM010000000
VERSION     DAHUWM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.309"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..969
                     /locus_tag="NK788_002765"
     CDS             <1..969
                     /locus_tag="NK788_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCH6771052.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="NK788_002766"
     tRNA            1159..1232
                     /locus_tag="NK788_002766"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          639 a          327 c          362 g          583 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DAHUWM010000304         1890 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHUWM010000304 DAHUWM010000000
VERSION     DAHUWM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.310"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            5..1150
                     /locus_tag="NK788_002767"
     CDS             5..1150
                     /locus_tag="NK788_002767"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCH6771053.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="NK788_002768"
     CDS             1321..1779
                     /locus_tag="NK788_002768"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCH6771054.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          619 a          356 c          375 g          540 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt
//
LOCUS       DAHUWM010000305         1883 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHUWM010000305 DAHUWM010000000
VERSION     DAHUWM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.311"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(571..861)
                     /locus_tag="NK788_002769"
     CDS             complement(571..861)
                     /locus_tag="NK788_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771055.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1069..1761)
                     /locus_tag="NK788_002770"
     CDS             complement(1069..1761)
                     /locus_tag="NK788_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCH6771056.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          506 a          362 c          291 g          724 t
ORIGIN      
        1 aattttcatt atatccagaa aaatttttct tagttaagga cctatggatt gcagtaatgc
       61 agaattcgtc gaataaactg ccaaagacat acaatcatat tgcttttaaa attgacgaac
      121 aagatataga ttcgtttgtc tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac
      181 gttctcgtgt gaaaggcgag gcttcttcaa tttactttta cgactatgat aatcacctct
      241 tcgagttaca taccggtact ctccaggaac gtttaaaatc ctacaattcc actacctgag
      301 actagtaatg actagtctct ttttatggcc tctgatcccc ccattgagcg aatactaata
      361 gggcttcgaa ttgatctgtt caatctttaa gcaatatccc tcaaatgagt ttggaaagtt
      421 tagtgattca tcacaaatac ttaaatacat gtagttatat atagattgag gttcttgtga
      481 gcgtatgctc gtgtgggttc aaatcccatc gtctgcatcc atctttccaa aacaagcggc
      541 taggacattt ttgtctcagc cgcttgtttt ctagtttatt ctttcatctt gtcggacctt
      601 ttttcttggg tatagacttt tctcatattc taccacaatt ttgggtttcc ctaacacatg
      661 ataggatctc acaacaaatt gctttctatt attataataa cctgtaacga ctaacttcat
      721 cccttcgtct acttcagcta ggaagttcaa actatgggaa gcaatcaaac aattgacccc
      781 atttaaactg aaacggatca acggtctggc actaacttgc aaagtacgga tcttatctac
      841 tgttccatat tttttctcca taaccttcac ctcaatacca ttatacgaac atacattccc
      901 tttgtaaaga agaaactctg ttttatcttt ttgacttttt gtctattacc tttcttaaaa
      961 tcatattggc acttcctgcg aagatgaagg acatgtacta aaaaaaagag gcagcagtat
     1021 agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt atttacgcaa
     1081 atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat cttttgcttt
     1141 ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt cgtctaattt
     1201 cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca gtgttgcgat
     1261 ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt gtgcttcttg
     1321 tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt ggttgatctc
     1381 atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct tgctgactaa
     1441 ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt ctaattcatt
     1501 tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct gttctttttg
     1561 tgctagtcga gcggtaagat ctgtaagcgc ttgctcttta tctgtcaatg tatgatcaag
     1621 ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca aaataatttc
     1681 atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga caatcccacc
     1741 agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta catgctaaaa
     1801 gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catgtgtaat tttaatataa
     1861 atactatcaa atactcattt aat
//
LOCUS       DAHUWM010000306         1881 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHUWM010000306 DAHUWM010000000
VERSION     DAHUWM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.312"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(18..239)
                     /locus_tag="NK788_002771"
     CDS             complement(18..239)
                     /locus_tag="NK788_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771057.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(337..582)
                     /locus_tag="NK788_002772"
     CDS             complement(337..582)
                     /locus_tag="NK788_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771058.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            822..1436
                     /locus_tag="NK788_002773"
                     /pseudo
     CDS             822..1436
                     /locus_tag="NK788_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          645 a          324 c          280 g          632 t
ORIGIN      
        1 ataaaaagag cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct
       61 agaatcactt caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac
      121 ttcttttctt tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt
      181 gctctttgtt caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt
      241 attgatccct cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc
      301 ttagatctaa gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc
      361 gtcttttgct ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc
      421 attaattaca cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat
      481 tttaattcca tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc
      541 acctatctct tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt
      601 caaaagtaat aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat
      661 tctttttctt ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta
      721 gagtttaccc taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat
      781 tagggtgtat tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta
      841 gagtttcatc catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg
      901 ctgaaaaaat ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc
      961 aggaagcttt agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac
     1021 ttggacgcaa ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc
     1081 gacttattat caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata
     1141 aattcatcaa agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg
     1201 aatcgaaacg acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag
     1261 gtcgtcctaa gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga
     1321 gtatcgttca ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg
     1381 tgactaggca aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc
     1441 ttttttgaat cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat
     1501 tcatctagct aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt
     1561 gatgcataaa tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct
     1621 tatgtaaact aataagattt gaattttatt ttatacacac tcgataatag atcaatttta
     1681 tggtcccccg catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt
     1741 ttcaattcaa acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt
     1801 aaaatttaaa acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga
     1861 gctttgtatt tctccataat a
//
LOCUS       DAHUWM010000307         1860 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHUWM010000307 DAHUWM010000000
VERSION     DAHUWM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.313"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(405..902)
                     /locus_tag="NK788_002774"
     CDS             complement(405..902)
                     /locus_tag="NK788_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCH6771059.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(942..1751)
                     /locus_tag="NK788_002775"
     CDS             complement(942..1751)
                     /locus_tag="NK788_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCH6771060.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          535 a          347 c          324 g          654 t
ORIGIN      
        1 ttcgagtcct acaaggtaca taagaggttg tgaaaaagct gccctgcatc aagtaataag
       61 aacagccaag taaaaacagc ttcttatgtt tttacttggc tgttcttatt actgcagatg
      121 caagcttttg aataccgttt aaggaagagg ttgtgaaatc agcgttttga cctgagtatt
      181 agcttaacaa acggaaaaat agttcctcat attttgccgt ttgttaagct aataccgagg
      241 gtcagctgat tgaacaccat ttaagaaaga ggtcgtgagc ctgccgtttc actctgtgtc
      301 ataaaaaagc caatctactt tgatagaaaa agcacgtagg actaaaacag ttctgcgtgc
      361 tttttttatt gtgtgttaaa tatcgtaaat gaagatagat atttttatga ttcgacgata
      421 gagacgttga aatctttcaa cagctctaaa gcggattctc cgtgttttct actggagact
      481 aacattggat caacaaaaaa gtctgcttca ggaaaagcac tctgcaggcc tatcagatta
      541 gagagtacac acaagtttgt ttcaatcccg actacttcga tctctttttg ttcttctttg
      601 ctttcaaata aagtttcctg gatttcagct aaagtttcag gtggaatcgt aaagtaatac
      661 tttttaatca ctcgttcatt ttcttgtgga gaaagaagag ggatcaattg caaatcttct
      721 cgttcttctt ctttgtcctt cagttcaact ggtatatccc gtgtgaagag aatatattca
      781 ttattttggc gtgctttagc taatctttga gcaatacgtt ctgccaattg atctgactct
      841 ggaatataaa attcgctgtt tggatcaaga atatgatttt gcatatcaat aataactaac
      901 attttctttc ctcctacatt catatcgtca ttttattctt tttaatagaa gttttttaaa
      961 atataagcaa caccagcttc ttcattcgtc agtgttactt ggtttgcaat ttcttttact
     1021 tgtcgctgtg cattccccat cgcaatactc aacccgctgt aattcaacat agcaatgtcg
     1081 ttttcttgat cgccaaaagc catgatgtca ctcgattcga ttccaaggta atccgccaga
     1141 aaaacaagtg aatgagcttt agtgatacct gtaggcgtga tttctaagaa atgcggatca
     1201 gagaatactg attggatacc atctgcttgc agatctcttt gtagcttctg tagtttttct
     1261 tcactcttat ctgttaatgc taatttaaga tagctaagag aaggatcttc caacatattt
     1321 aaaatttgat caaaagaatg gttctgtaaa ttgaaatgtt cataataagg gtcatctttg
     1381 actagatttg attcatcgaa tgtcagctga tcatcttggg tttctcggct agcaatgatg
     1441 gatattttcg cttcttgagc atagaagaag gcttttctca gtgtatcgct atctattttt
     1501 gaacgtctga gaactttcgc catttttcca tccttcattt cagcaatcag tgaaccattg
     1561 gaagaaatca aatagccacc gattcctaat tcggaaaaaa tatctttagc agaaatgctg
     1621 ttccttccgg tagcgacggt tacgattccg ccattttcat caaatttccg aagtgcttgg
     1681 atattttctt ctggtatctt tccatttcga tcaagcaagg taccgtctaa atcaatcgct
     1741 gctagtttca ttttatcttc ctactttctt tttacatatc tattttcctg ttaccttaag
     1801 attaaatgag aatttaaaaa aatgaaacta acttgctttg tcattatgaa ttttttcaaa
//
LOCUS       DAHUWM010000308         1830 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHUWM010000308 DAHUWM010000000
VERSION     DAHUWM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.314"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..470
                     /locus_tag="NK788_002776"
     CDS             <1..470
                     /locus_tag="NK788_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCH6771061.1"
                     /translation="KIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEIS
                     IGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVG
                     TQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            460..1602
                     /locus_tag="NK788_002777"
     CDS             460..1602
                     /locus_tag="NK788_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCH6771062.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1615..>1830
                     /locus_tag="NK788_002778"
     CDS             1615..>1830
                     /locus_tag="NK788_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6771063.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPI"
BASE COUNT          641 a          245 c          381 g          563 t
ORIGIN      
        1 aaaaaatcta taaatttatt gtagctattg gtgactgtgt agtaagaaaa aagattataa
       61 aaaaattgaa tatgccaagt gagcaatatg gaatagctgt tcatccaagt gctatagtgg
      121 caaaagaaat ttcaattggt tatggaactg ttatttttgc gaatgctgta attaacccag
      181 atgctgtaat tggagagcat gctataataa acacaggtag cattgttgag catgattgta
      241 gaattaataa ctatgttcat ttatctccag gtgtatgctt agcgggtgga gtacatgtag
      301 gtgtaggaac tcaaatagga attggttctc aatgtatcca gatgaagaaa attggatctt
      361 ggtgtatgat aggcgcaggg agcactattg ttaaggatat tccctcacat tccttagctt
      421 atggcaatcc agctaaaatt aagaaagaag gaattaattt tgaattctag aattttatta
      481 tcaagtcctc atatgggagg aacagaagaa aaatatgttg ctaaggcttt taaagataat
      541 tggatagcgc cattaggtga aaatgtggat aaatttgaag aaagtattac aaaatatact
      601 ggttctaagg gcggagcttc tgcgttaagt tcaggtacag ctgccataca tcttgcctta
      661 agactgctta atgttggaaa aaacgatatt gtctttgctc cttcatttac ttttatagct
      721 acagtaaatc ctattttata tcaaggggct gtaccagtgt tcattgactc agaacctaat
      781 acatggaata tgagccctgt tgcactagag agcgcattag aagaagctga aagaaacaac
      841 caattaccta aggcaatcat cgtagtgaat ttatatggtc aaatggctga ttatgaacga
      901 attttagcta ttgcaaacca atttaatgtt ccagtgatag aagatgcagc ggaatcgtta
      961 ggttctactt ataagggaat ttcgtcaggc ttatttggga caattgggat ctattcattt
     1021 aacggtaata agatcatcac aacctcaggt ggaggagctc tagtgtctag tgataaaaaa
     1081 tatgttgagc attcaagatt tttagcaaca caagcaagag atcaagcacc gtattatcaa
     1141 catagcgaaa taggttataa ttaccgtcta agcaatattt ctgccggtat cggacgtgga
     1201 caaatggaag tattgaatga tagagtagct aaacgaagag aaattttcaa tcgatataag
     1261 gaagcttttt ctggaattga tagaattgaa atgatgccag aagtggatgg catggtgcca
     1321 aatcgttggt tgtctacttt aacgatcaat gattataatt ctattgatca agtagttaac
     1381 gtaataaata aactaaatga aaaaaacatt gaaacaagag ctttgtggaa accactacat
     1441 ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg ataataatct tagtgtgtca
     1501 gaaaagttat ttaatagcgg cttatgctta ccttctggaa gtaatatgac agatagtgaa
     1561 caagaatttg ttattgatga gtttaaaaaa atattcaagt aggtgtgata ttttatgaac
     1621 ataggaatta ttggtttagg ttccatgggg aaaagaagac ttagactcat tcaagaacac
     1681 tttaatgata ttttactatg tggtgttgat actaatgagg cgagacgaaa ggaagttgaa
     1741 aaggaatttg gaataccaac ttataatagt ctaacagagg caaaaaattt gtttgaaata
     1801 aattcagttt ttgtttgtac atcccctata
//
LOCUS       DAHUWM010000309         1827 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.315, whole genome shotgun sequence.
ACCESSION   DAHUWM010000309 DAHUWM010000000
VERSION     DAHUWM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.315"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(30..731)
                     /locus_tag="NK788_002779"
     CDS             complement(30..731)
                     /locus_tag="NK788_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCH6771064.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(747..1187)
                     /locus_tag="NK788_002780"
     CDS             complement(747..1187)
                     /locus_tag="NK788_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCH6771065.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1187..1762)
                     /locus_tag="NK788_002781"
     CDS             complement(1187..1762)
                     /locus_tag="NK788_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCH6771066.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          513 a          400 c          282 g          632 t
ORIGIN      
        1 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
       61 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
      121 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
      181 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
      241 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
      301 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
      361 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
      421 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
      481 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
      541 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
      601 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
      661 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
      721 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
      781 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
      841 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
      901 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
      961 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
     1021 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
     1081 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
     1141 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
     1201 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
     1261 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
     1321 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
     1381 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
     1441 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
     1501 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
     1561 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
     1621 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
     1681 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
     1741 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
     1801 tacacgtgtt tttttaaaat aaaagac
//
LOCUS       DAHUWM010000310         1800 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHUWM010000310 DAHUWM010000000
VERSION     DAHUWM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.316"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            77..1642
                     /gene="guaA"
                     /locus_tag="NK788_002782"
     CDS             77..1642
                     /gene="guaA"
                     /locus_tag="NK788_002782"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCH6771067.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          555 a          374 c          386 g          485 t
ORIGIN      
        1 aggtctgatc tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac
       61 taagttttat tcaaatttgt cttcaccaac acgatttgac gaagagccaa aaattatcgt
      121 acttgattac ggtagtcagt ataaccaact gatcactcgc cgtattcgtg agttcggtgt
      181 tttctcagag ttgatgagcc accgcattac cgcagaagaa gtaaaggcaa tgaaccctaa
      241 aggaatcatc ctttctggcg gaccaaacag tgtttatgat gagaaggcct ttggtattga
      301 tgaaaagatt tttgatttgg ggatcccagt tttaggaatc tgttacggta tgcagttgat
      361 tacttataaa ttaggcggaa aagttaaacc agccaaaaac cgtgaatacg gaaaagcaga
      421 attggaaatc acttctgcag aagcggccct tttccaaaac actcctgaaa aacaaaccgt
      481 ttggatgagt catggtgacc tagttaccca agcaccgact gatttcgatg tcgttgctac
      541 cagcaaagat tgccccatcg catcgatcca aaatgcagaa cgcaaaatgt acggaatcca
      601 attccatgca gaagttcgcc attccgaata tggaaatgac ttgttgcgcc attttacact
      661 agatgtctgc caatgtactg gtgactggac aatggataac ttcatcgaca tggagatcgc
      721 aaaaattcgt gaacaagtcg gtgacaaaaa agttttatta ggcctttcag gcggagtaga
      781 ttcctctgtt gttggtgttc ttttacaaaa agcgatcggc gatcaattaa catgtatctt
      841 cgttgatcac gtattgttgc gtaaaggtga agcagaacaa gttatggaaa gtcttggcgg
      901 aaaatttggc ttgaacatta taaaagtcga tgccaaagaa cgtttcttat caaaacttgc
      961 tggcgtgtct gatccagaag aaaaacgtaa aatcattgga aatgaatttg tatatgtctt
     1021 cgatgatgaa gcaaccaaac ttgccggtga agaaggcgtt tctttccttg cacaaggaac
     1081 cctttatacc gatgtgatcg aatcaggaac tgaaactgcc caaaccatca aatcacatca
     1141 caatgtaggt ggattacctg aagacatgca attcaagctg atcgagccat tgaacacatt
     1201 gttcaaagac gaagtacgtg aattaggtac ccaattaggc atgcctgaca gcatcgtttg
     1261 gcgccaacca ttccctggac caggcttagg tattcgtgtc cttggtgaat tgacagaaga
     1321 aaaattagaa atcgtccgtg aatcagacgc tatcttgcgc gaagaaatcg ccaatgccgg
     1381 tttagatcgc gacatctggc aatacttcac cgtcctacca ggcattcgct ctgttggggt
     1441 tatgggagat ggccgtacgt atgactacac tgtaggtatc cgtgctgtca cttctattga
     1501 cggcatgacc gctgactttg cccgtatccc ttgggaagtc ttgcaaaaaa tcagtgtccg
     1561 tatcgtaaat gaagtcgcac acgttaaccg tatcgtgtat gatattacca gcaaaccacc
     1621 tgcaacagtg gagtgggaat aaagagccaa cataatatgg atgaggaata cctataaatc
     1681 aacattattc aattactaaa gttcctgatt ttcgtaaaaa ataagatggt ttcctaccat
     1741 tttccctacc aaagcccttg ttagattaac tactaacaag ggctttgctt ttattcacta
//
LOCUS       DAHUWM010000311         1795 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.317, whole genome shotgun sequence.
ACCESSION   DAHUWM010000311 DAHUWM010000000
VERSION     DAHUWM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.317"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..193)
                     /locus_tag="NK788_002783"
     CDS             complement(<1..193)
                     /locus_tag="NK788_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6771068.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREE"
     gene            487..627
                     /locus_tag="NK788_002784"
     CDS             487..627
                     /locus_tag="NK788_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771069.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            925..1182
                     /locus_tag="NK788_002785"
     CDS             925..1182
                     /locus_tag="NK788_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCH6771070.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1153..1461)
                     /locus_tag="NK788_002786"
     CDS             complement(1153..1461)
                     /locus_tag="NK788_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771071.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1539..>1795
                     /locus_tag="NK788_002787"
     CDS             1539..>1795
                     /locus_tag="NK788_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCH6771072.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSA"
BASE COUNT          646 a          304 c          306 g          539 t
ORIGIN      
        1 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
       61 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
      121 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
      181 caaacgttat catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta
      241 aaattcgttg actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa
      301 ctaaaaagac aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta
      361 aaatcaatgt ttatatgtct tatttaacta tgttctgatt ttaacactgt atgttaattt
      421 agtcaacgca gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag
      481 aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa
      541 caagcaaaag tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc
      601 atagcagaga ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca
      661 tagataacag cataatgaat atctaaataa gcttgaacaa tttcttttgg cgaaattgat
      721 tcgcctttag tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg
      781 atttcaaacg gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt
      841 cgtggtaata ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa
      901 tcatttatta aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa
      961 aaaatgatag atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa
     1021 ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg
     1081 aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac
     1141 gagcaagaat tactatttga aaccgatact tttaacagct gatgttttat agatgacagt
     1201 ttcatccgaa tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat
     1261 aagacctaca agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt
     1321 attcacgtaa atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc
     1381 atcgtatcca acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt
     1441 aattacaaat tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca
     1501 ctgataagga aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta
     1561 atttcgagca aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa
     1621 aagatgtagc agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg
     1681 acttggagga taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga
     1741 ttaacgagtc aggcctttac agtttaatct taaaatcaaa acttccctct gccaa
//
LOCUS       DAHUWM010000312         1788 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHUWM010000312 DAHUWM010000000
VERSION     DAHUWM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.318"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..1557)
                     /locus_tag="NK788_002788"
     CDS             complement(<1..1557)
                     /locus_tag="NK788_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCH6771073.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVIL"
     gene            complement(1576..>1788)
                     /locus_tag="NK788_002789"
     CDS             complement(1576..>1788)
                     /locus_tag="NK788_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HCH6771074.1"
                     /translation="MLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQA
                     TFQLNDLNKAFLASHIYGKILSAKFE"
BASE COUNT          565 a          331 c          239 g          653 t
ORIGIN      
        1 taaaataacc aaaacatttt tttgaatttt tgttaaataa atatccccta tttgaataaa
       61 acgattgact aattgaactt ctcgactaca ccaactatta atatcattga tgaaggaata
      121 taatttatcg tccgatgtta attggatttg ataaatccaa gaattcaaaa agatagacgt
      181 tatttgaaga taataatagt caaattctat ttgaaaatct ctctctattt ccttaacata
      241 ctgtctaaat cgaatctctt gataggtggg atatgttaaa gctgaattat tttggatatc
      301 aacattcttt tgtctaaaat atctatcata tttctcaaaa agctgctgct tatcagtgtc
      361 tttcttccac gtttgataaa gatactctat aagttcgttg atattaaaaa cttcgtctac
      421 tttttccaaa tcatccattt gaatatattt taattcattc tgtcttttca taaccttcat
      481 gccgttgaaa ttcacccatt gtggtaaatg atacatctga atataaagtt cttccaaatc
      541 atctaagaaa taaaaggcat tggcacaggt caattgaatg atatttttca aattgccaac
      601 attttcacta aagcgatagt ttaataaaaa atctaataat tgttgagata tcagtatttt
      661 ctttgctagt ctttcttctt ctgatttaaa aaatatcttg attaactgtt ttctttcata
      721 aagagaacga ctgtctaatg ttggaagtgt tagctttaca ggtattcttc tcaacaaagt
      781 tgataatagt ttatcctcag gtggttctga tgttgcaaat ataatccagc aacttgatgt
      841 gtaccagttt tcattatcac ccatccgatg aaatttcctc gtatccataa actgaaaaag
      901 tttctcctga cattcattgc tcaatgcatg aatttcatct aaaaataaga ctccattatt
      961 agctaattct gctaaaccct ttcttccatc ttcggctcct gtatatgcac cttttacatg
     1021 cccaaaaaaa ttggacaaga aaagttctgg attattggca tattcactac aattaactga
     1081 aatgaaagat gccttctctt ctatataatt attggccaac aaatccttat acacataatt
     1141 tgctaaatag ctttttccag ttccagtctc gccatgaagt aaaatgggta agccatttgg
     1201 cggatagtag attgctgctt taatttgagc aattacattt cgcagacttc cttgacttcc
     1261 gataaccctt tctattccaa ccctaaaaga gctatcatac catttttccg cttcctctga
     1321 agagctaaaa atttgttgat ttggaacttt ctccggtaaa gaaaagaaga ttactggacg
     1381 agtattgatt tttccaaatt ctccatcttt tacgaattca ttcaaatatt gagagacaat
     1441 attacgacta acatttaatt tttcagatat tttctgtgct gtattgttcc cccatttatt
     1501 tttaatctca gagttctcta tgacataatt gacaatgcgt tcttttaatt cttccatcac
     1561 agcacctcct cgacactatt cgaattttgc cgacagtatt tttccataaa tatgacttgc
     1621 taaaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     1681 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     1741 cagatcttct tttgattctt catctgaaag taattcacga tacaacat
//
LOCUS       DAHUWM010000313         1776 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHUWM010000313 DAHUWM010000000
VERSION     DAHUWM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.319"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            23..499
                     /locus_tag="NK788_002790"
     CDS             23..499
                     /locus_tag="NK788_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771075.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(505..687)
                     /locus_tag="NK788_002791"
     CDS             complement(505..687)
                     /locus_tag="NK788_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771076.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(687..986)
                     /locus_tag="NK788_002792"
     CDS             complement(687..986)
                     /locus_tag="NK788_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771077.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(1091..1294)
                     /locus_tag="NK788_002793"
     CDS             complement(1091..1294)
                     /locus_tag="NK788_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6771078.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1309..>1776)
                     /locus_tag="NK788_002794"
     CDS             complement(1309..>1776)
                     /locus_tag="NK788_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCH6771079.1"
                     /translation="SEVLPTIRKTGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEY
                     EPKISYLDTILSSTDTVATSQIAADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMN
                     QGYTKSHTSEIPKADGGTKVVMNTKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT          502 a          347 c          285 g          642 t
ORIGIN      
        1 cgttagaaag tgaggtgatt ctatgaggaa aaagctcaat tacattctta ctgcgacttt
       61 aaccatatat ataattctgt ttattacctt atctttcctg tcttcaatta aaaaaattcc
      121 ttttattaca ttgaataaaa atagtatttt gaaggagttc aataacactg actacatcgc
      181 atttctaaaa cttttttcag tctgtgtgct aatatcatgt gtaattattt ttattgttgc
      241 taaaacatgt tggacaatgt taaataaaaa ccaacgcgat gttgaaaata ttgctatatt
      301 tttaattaag attacttcct caatatcaat tatcattccg atgactgtaa ctttttcaaa
      361 agaacagttt aatgtagcgg ctacttatct ttcatttata gcgctatttt cttttgttct
      421 tcctaaaaga aataagagca actttgacaa caaggagctg gatgaagtac gcaatagcag
      481 cccaaaggaa caaaaataaa aaaatcatat tttagtcagt ccctcccgac tggctttttt
      541 cgctctgtgc tcagcttcat caagccccat aaaaatccaa accatgtaaa cgattgtccc
      601 tatcaacgct tgtctgcttc cccagaggcc taaagcgtag atgattagcg gtgcgctgaa
      661 tactagtgct ctgttgaatt tgcccattaa cttgcctcct tcaaaacatc tgaaggcgat
      721 aaacttttta cacgatattt gtttgcgtct ttccacttta aaaaccaaat aaaagtatgg
      781 taatgaataa atgtggttga gtgacctgga cgaagtattc cttctgcaaa ttcaggaatt
      841 gtttccattt ccttgcagta ttcaactaaa gtagtttttg acatgccgtg gaacttctcc
      901 aagattaaac tttgtcggta ccagtcgtct ggttttactg ccttctcagc agcttctatc
      961 agctctgaaa gtgttggttt tttcacttat tatccctcct aaaaattatc gttccatact
     1021 cgatgtttaa catttcttga agctctgatt ttgtaagaat gattttatct tccaacgact
     1081 ttacctccct ctacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg
     1141 cagaagtctt tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt
     1201 cagcaagcat ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc
     1261 tatctcgttg agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc
     1321 ctctaaatcc atttgagggt aatagccctc tgcgattaat aagttgtaaa taaacactcg
     1381 ccctttctgt gtccatttgg tattcattac aactttagtg ccgccatcgg ctttcggtat
     1441 ctcacttgtg tgcgattttg tgtatccttg gttcatatgt tttcggtaaa gtatccattg
     1501 tccgctaact ttatgctgaa cacctaactc gtttagcaat ttgtttagag caattgccga
     1561 cattccgtaa tcagctgcaa tttgagaagt agctaccgta tctgtcgatg aaagaatcgt
     1621 atctaagtag ctaatctttg gttcgtactc ggcaatttgt tgttcgagta attggttctt
     1681 ttcttctaaa tccgctgcta aacgcaatgc ttgtgcaaaa ctttgaggta cgtttgaata
     1741 gctacctgtt tttctaattg ttggaaggac ttcact
//
LOCUS       DAHUWM010000314         1766 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHUWM010000314 DAHUWM010000000
VERSION     DAHUWM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.320"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            194..790
                     /locus_tag="NK788_002795"
     CDS             194..790
                     /locus_tag="NK788_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCH6771080.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(848..1330)
                     /locus_tag="NK788_002796"
     CDS             complement(848..1330)
                     /locus_tag="NK788_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771081.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1436..1633)
                     /locus_tag="NK788_002797"
     CDS             complement(1436..1633)
                     /locus_tag="NK788_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771082.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          591 a          359 c          270 g          546 t
ORIGIN      
        1 attcctaaaa cacaacaatg aaagcgataa ctaagaaaat ccttacataa cgatttttct
       61 tattaaaatt gttttttgaa cttcgcttat tttttgtgag catatctgtt tacttacttt
      121 ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa atgcaaaacg caaatcaaag
      181 gagaaatttt attatgacaa cttttactga cactttgaaa aaccgccgtt caatctatca
      241 tctaggacgt aatgtttcac tttctaatga agaattaaca actttgatca aagaagcaat
      301 taaagaaagc ccaactgctt tcaatgctca atcaacacgc gcagttatcc ttttcggaga
      361 tgcacatgaa aaactttggg aaatcactga agaagcactt cgcccattga ctccagcaga
      421 agctttccca aatacacaaa acaaattagc tggtttcaaa aatggttatg gtacagtatt
      481 attcttcaaa gatactgacg tggtaaaagg tctacaagaa caatttgaat tatatgcaga
      541 caacttccca gattggtcag aacaatcaaa cggaatcgca acagctaata cttgggtagc
      601 attagttgat aaaggattgg gcgcaaactt gcaacattac aacccagtta ttgacgaagc
      661 agttgcaaaa gaatggaata tcccttcaaa ctggaaacta cgttcgcaat tagtattcgg
      721 ttcaccagaa acaccggctg gggaaaaaga atacatgaac gatgccgatc gcttccgcgt
      781 atttggataa ttttatttga aataaaaaac agaggttgtg acaaacatct tgtcgcaacc
      841 tctgttttta ttctggatca atcataatat caatctctgc ctcatcgttt aagcgttggg
      901 acaaaaatgg aactaattct tctaatgatg cagaaaattt cagcatcggt cctcgccatc
      961 cgtttcggaa aaaattcatt tttacgattt tagaaccttt tggctgatat aatacaattt
     1021 tattgatctc agaaaaagga acaccttttt tgtcaaagct attcaaaaca agtccttctt
     1081 ctgtgattcc tctactgtcc aacaaaaaac taataaaaat cattgctgac aagatccctt
     1141 ttgccaattc atctgctgta ggatgactga tgagtgtata ccaaatgact agtgacacta
     1201 agaagggtat caccaagtag acccatcgat ttcgcacagt ccgataaata atttcttttc
     1261 gaaccagcca aagaaataaa aacatcacaa caacgaataa aaacaataga accgtaaaga
     1321 atgttaccat gacaactcgc ctcgcttttt cttctccaga aaataggaaa agaatactga
     1381 tttaccttct caaagaaatt ggacttgcag cacgccgcac gtccaattgt tttctttatt
     1441 caaatgtatc atattttgca acaaccatgc ttgctttttc atctaataat tcttggtcct
     1501 ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt catttttgat acaacttcac
     1561 caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata ttgttctcct aaattaatca
     1621 tttcttccgt catcaaaatc ctcctaaact gctggaactt ttctccacat taccactgct
     1681 atttctttgg agatttaact cattgatttt tatttacagt taaaaatcaa ccttacaggc
     1741 attatagcgt actgttttcg ttttca
//
LOCUS       DAHUWM010000315         1761 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.321, whole genome shotgun sequence.
ACCESSION   DAHUWM010000315 DAHUWM010000000
VERSION     DAHUWM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.321"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(12..212)
                     /locus_tag="NK788_002798"
     CDS             complement(12..212)
                     /locus_tag="NK788_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6771083.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(517..1749)
                     /locus_tag="NK788_002799"
     CDS             complement(517..1749)
                     /locus_tag="NK788_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCH6771084.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          491 a          360 c          336 g          574 t
ORIGIN      
        1 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
       61 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      121 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
      181 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
      241 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
      301 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      361 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      421 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      481 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      541 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      601 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      661 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
      721 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
      781 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
      841 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
      901 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
      961 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
     1021 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1081 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     1141 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1201 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1261 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1321 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     1381 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1441 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1501 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1561 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1621 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1681 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     1741 agataacata ataaaaattc c
//
LOCUS       DAHUWM010000316         1748 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHUWM010000316 DAHUWM010000000
VERSION     DAHUWM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.322"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            299..1339
                     /locus_tag="NK788_002800"
     CDS             299..1339
                     /locus_tag="NK788_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCH6771085.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1588..>1748
                     /locus_tag="NK788_002801"
     CDS             1588..>1748
                     /locus_tag="NK788_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6771086.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          621 a          211 c          374 g          542 t
ORIGIN      
        1 ataaataaac ttgcgagaac cagtttaggg gtatatattt tacatgaaaa tcattatttc
       61 attgatggta tgaagtctag aggcttaata tgggatggga tatttggatt aggcaaaagg
      121 tttttagata gggatttttg gatttggttt ataattgcag ttgtagttgt gtgcataatt
      181 tgcatggcta tcgatgttgt aagagaaaaa tttgaaagac agatttttaa aattaagagt
      241 gtaaagaacc tgtgtaaact tatgaatcat gttataaata tatacccata attaataaat
      301 gaggagaaaa gaagtgaaag ttatatcatt cggagaatta ttattgagac ttgcagcacc
      361 cggttattca aagttatttc aaagagatag tttggagact acattctgcg gaggggaagc
      421 taatgtagcg gtatccttag caatatttgg atatcaatca gaatttataa caaagcttcc
      481 ggattcagat gttggatatg cggcagctaa tgctttgcgt tattttggag taaatgttga
      541 taatattata tttggagagg gacgcatggg actgtattat ttagaaaaag gagcagctca
      601 gagaccatct aaagtaattt atgacagaaa attttctgca atttcgttag ctgatagtga
      661 tgaatttaag tggaaggaga tcctaaacga tgcaaattgg tttcattgga cagggataaa
      721 tccagcattg tctgagaatc tctttgagac gtccttagat gcatgtcagg ctgccaaaaa
      781 aagaggtata acaatttctt gtgatttaaa ttatcgatcc aagctttgga catcagagga
      841 agcaaaagaa aagatggaac agatactacc attggttgat atttgtattt gtaatgaaga
      901 agatgcagaa aaagtgctag gaattaaagc tgagcataca aatattaata gtggagaatt
      961 aaataaagat ggctatgtgc aggtggctag ggagattcac aagaagtttg gatgtaggta
     1021 tgtagctaca actctgcgta gaagttattc ggcaagtaga aatggttgga gtgcaatgtt
     1081 atacgaagca gaaaaagata aagcgtattt ttccacagaa tacaacatac aaattgttga
     1141 tcgcgttggt ggtggagaca gcttttctgc tggtataata tatgcgttat gtgaaaatat
     1201 gtccggacaa aaggctattg aatttgcgac tgcatctagt tgtttaaaac atactataga
     1261 aggtgatttt aatcgaacta caattgagga tgtatataat ttaatgaata gcggtggaaa
     1321 tggtcgcgtg ttgcgttagt caacatataa agataaatca cagtaatttt tatggtgatt
     1381 atctgtctta gtagtgatta gaataacgtg aaaataaatt gttgagcgtt tatactgttt
     1441 tgaaaaataa atttaacata ttgaaaaata caaatatata cgtatattat aaagcgccga
     1501 ttgtaaaatt aagctagaca actaaaacag tcatttgtgc tacactcaaa atagttccga
     1561 ccaaagaatt ataaggagtg agtgcaaata acctatacct atcttacaac ggatgaactt
     1621 gtaataatag agtcttattt gaaaattaac caatctgttg ctaaaactgc gcattgcttg
     1681 aatcgttcaa gtcaaacgat ccataaagta tatctgttct tcaagcaaag aaaatcagcc
     1741 ttagaata
//
LOCUS       DAHUWM010000317         1728 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHUWM010000317 DAHUWM010000000
VERSION     DAHUWM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.323"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..182)
                     /locus_tag="NK788_002802"
     CDS             complement(<1..182)
                     /locus_tag="NK788_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCH6771087.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="NK788_002803"
     CDS             517..951
                     /locus_tag="NK788_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCH6771088.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="NK788_002804"
     CDS             974..1294
                     /locus_tag="NK788_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771089.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="NK788_002805"
     CDS             1435..1626
                     /locus_tag="NK788_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771090.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          653 a          260 c          363 g          452 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatcc
//
LOCUS       DAHUWM010000318         1712 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHUWM010000318 DAHUWM010000000
VERSION     DAHUWM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.324"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(1..>1712)
                     /locus_tag="NK788_002806"
     rRNA            complement(1..>1712)
                     /locus_tag="NK788_002806"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          366 a          534 c          359 g          453 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cctgttatcc ccagggtagc ttttatccgt tgagcgatgg
      481 cccttccatg cggaaccacc ggatcactaa gcccgacttt cgtccctgct cgacttgttg
      541 gtctcgcagt caagctccct tctgccttta cactcttcga atgatttcca accattctga
      601 gggaaccttt gggcgcctcc gttacctttt aggaggcgac cgccccagtc aaactgccca
      661 tctgacactg tctcccacca cgattagtgg tgcgggttag agtggccata acgcaggggt
      721 agtatcccac cagcgcctcc atcgaaacta gcgttccgat ttctacggct cctacctatc
      781 ctgtacatgc ggtacagaca ctcaatatca aactacagta aagctccatg gggtctttcc
      841 gtcctgtcgc gggtaacctg catcttcaca ggtactaaaa tttcaccgag tctctcgttg
      901 agacagtgcc caaatcgtta cgcctttcgt gcgggtcgga acttacccga caaggaattt
      961 cgctacctta ggaccgttat agttacggcc gccgtttact ggggcttcaa ttcgtacctt
     1021 cgcttgcgct aagcactcct cttaaccttc cagcaccggg caggcgtcag cccctatact
     1081 tcatcttacg attttgcaga gacctgtgtt tttgataaac agtcgcttgg gcctattcac
     1141 tgcggctgat cgtatgatca gcaccccttc tcccgaagtt acggggtcat tttgccgagt
     1201 tccttaacga gagttctctc gctcacctta ggattctctc ctcgactacc tgtgtcggtt
     1261 tgcggtacgg gccgctgttt tctcactaga agcttttctc ggcagtgtga catcaggaac
     1321 ttcggtacta ttatttccct ccccatcaca gcttgtcctt aagaaaagaa gcatttgact
     1381 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
     1441 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
     1501 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
     1561 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
     1621 ttctcacttc taagcgctcc agcagtcctc acgatctgcc ttcaacgccc ttagaacgct
     1681 ctcctaccaa tacacctaaa ggtgtactcc ac
//
LOCUS       DAHUWM010000319         1678 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHUWM010000319 DAHUWM010000000
VERSION     DAHUWM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.325"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(486..1238)
                     /locus_tag="NK788_002807"
     CDS             complement(486..1238)
                     /locus_tag="NK788_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6771091.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1253..>1678)
                     /locus_tag="NK788_002808"
     CDS             complement(1253..>1678)
                     /locus_tag="NK788_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCH6771092.1"
                     /translation="KNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRV
                     LGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKK
                     GHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          545 a          293 c          219 g          621 t
ORIGIN      
        1 gctgtagttg gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta
       61 cttgtcggat gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta
      121 attatctcaa atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta
      181 actaaaaaat gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt
      241 ccaccataca tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact
      301 attcatttgg cccttttttg gttccagacc atagaaatat tcctccagta actaatattg
      361 ctgttatgca cgtaactaat aacagctata aaaattggta caactttaat aggattcaga
      421 aaaatttgca ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa
      481 aaccactaaa cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta
      541 aagtttctgg ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta
      601 acgttaaaga tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt
      661 tcaagctcac taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat
      721 ctatgtggta attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt
      781 tgagaaccta tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt
      841 tcttgcgcta ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct
      901 tttgttacaa atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca
      961 tgagtagttg taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat
     1021 tctataccgt taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt
     1081 cggaattgtt tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta
     1141 aatactttat cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt
     1201 ataatttgat cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca
     1261 acaaaataac ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg
     1321 gattcttttt attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg
     1381 ttgttattcc ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg
     1441 atatcgaaat ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt
     1501 gactctctat agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt
     1561 caaatattgg aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct
     1621 tattgttatt tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcattttt
//
LOCUS       DAHUWM010000320         1672 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHUWM010000320 DAHUWM010000000
VERSION     DAHUWM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.326"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="NK788_002809"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="NK788_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCH6771093.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          456 a          292 c          337 g          587 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg ca
//
LOCUS       DAHUWM010000321         1621 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHUWM010000321 DAHUWM010000000
VERSION     DAHUWM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.327"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(27..1508)
                     /locus_tag="NK788_002810"
     CDS             complement(27..1508)
                     /locus_tag="NK788_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6771094.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          509 a          224 c          284 g          604 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 t
//
LOCUS       DAHUWM010000322         1584 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.328, whole genome shotgun sequence.
ACCESSION   DAHUWM010000322 DAHUWM010000000
VERSION     DAHUWM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.328"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(24..401)
                     /locus_tag="NK788_002811"
     CDS             complement(24..401)
                     /locus_tag="NK788_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771095.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(701..1582)
                     /gene="galU"
                     /locus_tag="NK788_002812"
     CDS             complement(701..1582)
                     /gene="galU"
                     /locus_tag="NK788_002812"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCH6771096.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          435 a          356 c          240 g          553 t
ORIGIN      
        1 tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact ttgttcgaga
       61 gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg agactttctt
      121 ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc aaatacgcaa
      181 gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac ggatgctgca
      241 taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat gataaataga
      301 ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag ccacttgagt
      361 atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac acaccttttt
      421 ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg gctttcattt
      481 ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat tagcataata
      541 ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga ctcaactgat
      601 ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc cgctttaata
      661 acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct gttcttagct
      721 ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa tgttgaagtc
      781 catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat ctttttcctt
      841 taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca tccgttagct
      901 gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt ttaggagtca
      961 gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca ggtttttcta
     1021 caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca ataattccgt
     1081 atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct tgtgtttctt
     1141 cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa tcatccccca
     1201 gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg gcatccccca
     1261 gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt tcttcaacta
     1321 aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg atatttgcat
     1381 caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg tcttcgattc
     1441 ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg attggaagca
     1501 tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca gcggcaggaa
     1561 tgactgcttt tttgaccttc actt
//
LOCUS       DAHUWM010000323         1573 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHUWM010000323 DAHUWM010000000
VERSION     DAHUWM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.329"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAHUWM010000324         1570 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHUWM010000324 DAHUWM010000000
VERSION     DAHUWM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.330"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            37..411
                     /locus_tag="NK788_002813"
     CDS             37..411
                     /locus_tag="NK788_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCH6771097.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(525..1154)
                     /locus_tag="NK788_002814"
     CDS             complement(525..1154)
                     /locus_tag="NK788_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCH6771098.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1288..1360)
                     /locus_tag="NK788_002815"
     tRNA            complement(1288..1360)
                     /locus_tag="NK788_002815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1325..1327),aa:Thr,seq:ggt)
BASE COUNT          553 a          228 c          270 g          519 t
ORIGIN      
        1 tgcttttgta agaaaaagaa agagggcgta ttagttatgt atcttaaaat tacggaacaa
       61 gcacaagaaa gattacaaaa atatattgat gaaggcgcaa cagttatatt ggatttagat
      121 gatggcgttg gggaatattc taaaatgggt gtttgctctt tagatactag ttttcgttta
      181 cttcttcttg ataaagaaca gagaaaaaat gattataaac tccaattaga tagtgatatt
      241 ggggatgttt atataaaaga ttattcaaaa atgtacatgg atgaacatat gaccctttct
      301 ttagatccac gtctaggtgt atttactctt gaaagcccaa gtggttcttt agacagtcat
      361 gttcaattag tagatctacg accagaaaaa ttctctggaa taaaacaata actttctctc
      421 tattgtccat aaaactagtc tcaatcaagc tgtattgaaa aaacaagaca acattcttac
      481 catggtaaat aaaaagacga cacattttgt cgtctttttt taaattaact attttcgata
      541 aaagatcgca tagcttgtgc cattatttct ggcttttctc tattcaattc atgttttccg
      601 tttttaataa agagtagccg gccattcgtt gaaagtttat ataaacgttt tgctgctggt
      661 ttattcgcat gatctttggt accgcataaa atcaatttag gaagtcgtga tgtacgaagc
      721 tgttttttgc agtctaatgt ttcaagtgaa cgaagtaggg taagggtttg ttttttagat
      781 agacctaatt tttcaaatat cttagaaggt acgcagtaaa aataaatatt ctggaaagta
      841 agcaataagc gattaggaga ctcaaattgt ggagcgacta aaacaacctt ttttaaatat
      901 ggagaaggag ttgcaagata gtttaaagca ataacagcgc ctaaagataa tccacataaa
      961 ataaaaggtt cctttatctg attcaattgt acttcgatgt atttggttaa gtcgtctaaa
     1021 gtagcagtgt ctgtgctgtt taaatgattg aacaatgaaa gacttttgca agaataacta
     1081 ttgggtagat aagagattac atctttccat gattgttcgt cttggcctag accatgcaaa
     1141 aaaacaattt tcatattgct ctccttttta tagttatcat atagactttc tttgtactat
     1201 taaaatataa acattcattt cttacgtcat aaaataaaaa aacagcgcag acacattgtt
     1261 aaaccaacat gttgcactgt ttaaggaagc cacttgccgg gcttgaaccg gcgacctctt
     1321 ccttaccatg gaagcgctct accagctgag ctaaagcggc attatcatta atacaaataa
     1381 taggataacg aatattaatg tatttgtcaa cagaattttt tcgaaaaaat gtgtttctct
     1441 aataggaaaa cagattttag aaaaaataaa aataacaaaa aatgcgaacg gtttctttga
     1501 taatataata aaaaaattca aaaaaagtat tgcgttattt agaagaaatt gatattatat
     1561 tcatgtactt
//
LOCUS       DAHUWM010000325         1561 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHUWM010000325 DAHUWM010000000
VERSION     DAHUWM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.331"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            81..797
                     /locus_tag="NK788_002816"
     CDS             81..797
                     /locus_tag="NK788_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCH6771099.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            816..>1561
                     /locus_tag="NK788_002817"
     CDS             816..>1561
                     /locus_tag="NK788_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HCH6771100.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAG"
BASE COUNT          601 a          188 c          303 g          469 t
ORIGIN      
        1 agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat gttattttta
       61 taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt cttaaaaaaa
      121 agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta aataacaaaa
      181 gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt tattatatac
      241 tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt agtaacccag
      301 atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt tccttagatc
      361 aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta gaggctgatg
      421 tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt gaaaaaggac
      481 tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa ttgcaagatt
      541 tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct agaacacagg
      601 atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc aatgtgatga
      661 ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt gagattgagt
      721 cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat aatcatctta
      781 ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag attttagatt
      841 gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat aatacaaaaa
      901 aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg tttctcacaa
      961 ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa ttagcacaga
     1021 taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat ggcgaatata
     1081 atatagagga tcttccagat tatgatggtt catctattga tggaattagg attgcgtttc
     1141 ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa aaaggctact
     1201 tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt ttagcagtaa
     1261 taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt tttggaatga
     1321 tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta gaaaaagata
     1381 tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat gcacaaaaac
     1441 ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac ggaatgggaa
     1501 gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat aatgcaggaa
     1561 a
//
LOCUS       DAHUWM010000326         1553 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.332, whole genome shotgun sequence.
ACCESSION   DAHUWM010000326 DAHUWM010000000
VERSION     DAHUWM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.332"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            191..445
                     /locus_tag="NK788_002818"
     CDS             191..445
                     /locus_tag="NK788_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCH6771101.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="NK788_002819"
     CDS             446..763
                     /locus_tag="NK788_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCH6771102.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..>1553)
                     /locus_tag="NK788_002820"
     CDS             complement(1397..>1553)
                     /locus_tag="NK788_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCH6771103.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          552 a          231 c          293 g          477 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa gaa
//
LOCUS       DAHUWM010000327         1537 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHUWM010000327 DAHUWM010000000
VERSION     DAHUWM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.333"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(40..>1537)
                     /locus_tag="NK788_002821"
     CDS             complement(40..>1537)
                     /locus_tag="NK788_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCH6771104.1"
                     /translation="IFVGLLTGIVVSLFRLSIEKLSDLVRSIYEMSREQPIYLIGLAI
                     CCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSMNWFSVLWKKFIGGVLSVGAGL
                     FLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSGASSGLAAAFNAPIAGLLFVLE
                     EIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYLGDLPSLPLKYYGSLVILGVLL
                     GILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLILPIGYLWPSTLGGGNQIVLAFG
                     HASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPILTLGALIGSFYGNIMVDFGMDP
                     IYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLNHLMPLGLAALVAYIVNDLLGG
                     NPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSLDGLMVRDFNWPKNMLLISIRR
                     GNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEAKSSITNLKQSF"
BASE COUNT          523 a          331 c          294 g          389 t
ORIGIN      
        1 tagatgaata gttgttgtaa aatagttttt ccatgttcgt taaaatgatt gcttcaaatt
       61 tgtgatagaa gatttagcct cgatttcctg cttgattctt ggcaagtttg cttcatcagt
      121 caagataacc aaaatatctc ctacattcat gatcgtatca ccgtgagtca agatttcctg
      181 atttcctctg cgtatagaga ttagcagcat atttttaggc caattgaagt ctcgtaccat
      241 taatccgtct aaagaactct ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt
      301 tagtgaggcg gtctgagtat ttccaagtag acgttccaat aatgattcgt aaattggatt
      361 accgccgaga aggtcattga cgatataagc aacaagagca gccaatccta aaggcatcag
      421 atgattaaga gagccaacca tttctgtcac taaaataatt gctgtcagcg gtgcttttcc
      481 aatcgctgtg aaataaccag ccatggcaat aacgacaaaa tccttgatgt agattggatc
      541 catcccgaaa tcaaccataa tgtttccata aaagctaccg attaaagccc ccaatgtcaa
      601 tatcggaagg aaaatccccc ctggaagacc cccaccgtaa gaaatcatgg aaaaaacgaa
      661 tcgaagaaca aacaaaccga taatcataat gattggcaag gaagcatgtc caaaagctaa
      721 aacgatttga ttgcctccgc ctagagtact aggccataaa taaccaattg gcaaaatcag
      781 caagaaagga acgaacccgt gaaaataagc tggtaaaggc aatcggttgt aaaattttgg
      841 caaagccagc aagacattct gataaataaa acctaagatg cctagaagta ctcctaagat
      901 cactaaggaa ccataatatt tcaacggcaa agaaggaagg tcacctagat ataaaacagg
      961 tctcaagccg aaaaagtaaa gagagacgaa gttagcagtg attgctgaag caaatgatgt
     1021 cagccaaaca agtggagaaa aggagtgatg gatttcttct aaaacaaaga gtaatcctgc
     1081 gataggagca ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt
     1141 ttcttctgaa gatggactcc gaaacaattg tccagctcct tgtccaatgg atgcgccaag
     1201 ctgtatagaa ggtccttctc tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc
     1261 aatgaacttt ttccataaaa cagagaacca gttcattgat aattccccgc gaagctgtcc
     1321 ctctacttgt ggaatcccgc ttcccttgat atccggttca tttttgacca gataacccac
     1381 gaaaaatgct gcaaggatgc agcaaatagc taggccaatt aagtaaatcg gctgttcccg
     1441 actcatttca tagatacttc gaactaaatc gcttagcttt tcgatagata atcgaaacag
     1501 gctaacgacg attcctgtca gcagtccaac aaatatt
//
LOCUS       DAHUWM010000328         1530 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHUWM010000328 DAHUWM010000000
VERSION     DAHUWM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.334"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..395
                     /locus_tag="NK788_002822"
     CDS             <1..395
                     /locus_tag="NK788_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771105.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="NK788_002823"
     CDS             398..1147
                     /locus_tag="NK788_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6771106.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="NK788_002824"
     CDS             1159..1428
                     /locus_tag="NK788_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771107.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
BASE COUNT          607 a          253 c          304 g          366 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca
//
LOCUS       DAHUWM010000329         1513 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.335, whole genome shotgun sequence.
ACCESSION   DAHUWM010000329 DAHUWM010000000
VERSION     DAHUWM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.335"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..393)
                     /locus_tag="NK788_002825"
     CDS             complement(<1..393)
                     /locus_tag="NK788_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCH6771108.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSP"
     gene            complement(436..1368)
                     /locus_tag="NK788_002826"
     CDS             complement(436..1368)
                     /locus_tag="NK788_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCH6771109.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          420 a          320 c          250 g          523 t
ORIGIN      
        1 tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt gaagaaccta acgtgccata
       61 agacgctccg gtaatcatca ctggtttatc cacaaacgga aaaataccat atgaaagcca
      121 atttaatgcg ttaatcaatg aagcaggaac cgcatggtca tactctggtg tgctgatgat
      181 tacgccatct gcttgttcaa tttttccagc aatttcttta acaacatttg gtagttctgt
      241 gttttcaggt ttgttaaata gtggcagctc cttgatttcg acaatttcga tttctgcctc
      301 atttacaaaa taatttttca taaactgtaa taattcacgg tttgttgacc gcgttgaatt
      361 tgttcctact aatccaataa atttcatctt cattctcttt cctttctaac atcgatttct
      421 gactgttcgc acgtactaaa ttttttcaaa tccttttgta tacaagaatt gattctcttt
      481 tgttacaact gctccctcga tacccggctg ttcttcaagc gctttcaaca cttctaaaga
      541 agatgctcca aataaacgtg tagtccagat ttctccatct acagaagaat cagacaaaat
      601 cgtcaggctt gctacgtcat ttttaactgg aaagccagtt ttcggatcaa tgatgtgatg
      661 atacactgac gaaccatttt gtagataacg ttcgtagatt ccagaggtaa ctaaagattg
      721 gttgtttagt ttaaccaccg caagcgactc ttcacgtgaa cctttaggat ttcgcaaacc
      781 aatacgccaa gcaccatccg aacgtcttgg tgattcacca aaaacaacga tatttcctcc
      841 taagttaact aaagcaaacg ctgcatttcg ctctcggcaa tgtgtaatta atttatctgc
      901 aatgtaacct tttgctaacg cccccaagtc aatcatcatt cctggtttgg ttaaaaaaac
      961 ggttttcatt ttttcatcta attgaatttg gtgtggatcc gttagcagaa gtaactcagt
     1021 tatctctgct tttgttggaa ccaatgcatc ttcaaatcca atgtgccatt tttgtacaac
     1081 cggaccaata gcaatattca gataactatt tcttgcacaa ctatgtgctt ttcctaattt
     1141 gatgagctca aaaagttcag gatgtacaat cacagcttgt cgtccagcag cttgattgat
     1201 ggccattaat tcagaagttg catcatgtgc gctgaagcga tgttcataaa cgtgcaacca
     1261 ttcgcctaac tctgctagta atcgactagg ctcttcatgc ccaacttgta atgtaattgt
     1321 tgtccccatc atttgccaga ttttcgtttc ttcgatcacc tgattcattt tgtaccttct
     1381 ttacttgaac tacggattac atgagtaatt agtttttatt tttgttactg ttttcacaac
     1441 cagatgatac gcctgctaga tttatttgtc aaccgatttt atatattttt ttatataata
     1501 aggtaatttt aat
//
LOCUS       DAHUWM010000330         1509 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.336, whole genome shotgun sequence.
ACCESSION   DAHUWM010000330 DAHUWM010000000
VERSION     DAHUWM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.336"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            101..868
                     /locus_tag="NK788_002827"
     CDS             101..868
                     /locus_tag="NK788_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCH6771110.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="NK788_002828"
     CDS             1060..1458
                     /locus_tag="NK788_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCH6771111.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAHUWM010000331         1504 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHUWM010000331 DAHUWM010000000
VERSION     DAHUWM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.337"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1457
                     /locus_tag="NK788_002829"
     CDS             <1..1457
                     /locus_tag="NK788_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCH6771112.1"
                     /translation="QDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANG
                     GLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALP
                     FGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAP
                     LIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFI
                     RNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEK
                     PRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIF
                     ILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLG
                     SFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGT
                     ESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          408 a          288 c          318 g          490 t
ORIGIN      
        1 agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga
       61 tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat atgtacgata
      121 cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct
      181 acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag
      241 gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg
      301 ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg
      361 cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc
      421 gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac
      481 cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg
      541 ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg
      601 gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta
      661 acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc
      721 atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt
      781 attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat
      841 gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt
      901 catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga
      961 tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat
     1021 tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc
     1081 ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg
     1141 gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg
     1201 ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat
     1261 ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg
     1321 cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg
     1381 cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa
     1441 tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc
     1501 tgtt
//
LOCUS       DAHUWM010000332         1502 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.339, whole genome shotgun sequence.
ACCESSION   DAHUWM010000332 DAHUWM010000000
VERSION     DAHUWM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.339"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            138..476
                     /locus_tag="NK788_002830"
     CDS             138..476
                     /locus_tag="NK788_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771113.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(681..1256)
                     /locus_tag="NK788_002831"
     CDS             complement(681..1256)
                     /locus_tag="NK788_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCH6771114.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          464 a          312 c          256 g          470 t
ORIGIN      
        1 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
       61 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
      121 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
      181 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
      241 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
      301 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
      361 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
      421 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
      481 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
      541 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
      601 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
      661 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
      721 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
      781 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
      841 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
      901 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcata aaagccactc
      961 attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact
     1021 gtctctgcac gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc
     1081 atgatttccg gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac
     1141 ggaaaaactt ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct
     1201 cgataataat tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta
     1261 atccctccca atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg
     1321 ctttcaattt ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt
     1381 attctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt
     1441 tttgaaattg ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg
     1501 gt
//
LOCUS       DAHUWM010000333         1486 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.340, whole genome shotgun sequence.
ACCESSION   DAHUWM010000333 DAHUWM010000000
VERSION     DAHUWM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.340"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            95..1084
                     /locus_tag="NK788_002832"
     CDS             95..1084
                     /locus_tag="NK788_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6771115.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1066..>1486
                     /locus_tag="NK788_002833"
     CDS             1066..>1486
                     /locus_tag="NK788_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCH6771116.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQK"
BASE COUNT          539 a          193 c          276 g          478 t
ORIGIN      
        1 aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga taataggtat
       61 tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa tgagacaact
      121 acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa ttgatcatgt
      181 atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat atctatttga
      241 taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact atgctatacc
      301 aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca caacaccaga
      361 aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt gtttatctaa
      421 tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg attggggtaa
      481 agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat ttaatatttt
      541 acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg ataagttaaa
      601 tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat ggggtaaaaa
      661 ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg gagaaattta
      721 ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg ctattcattc
      781 tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat tcatcttagt
      841 gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca ctaaaaaatt
      901 cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta tagatagtgt
      961 agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa tggtagaagc
     1021 gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc aaatgaaata
     1081 ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt gattacaaca
     1141 cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat gaatgacgcc
     1201 acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt tgaggagagt
     1261 atgaaagaaa taattagtca gcatcatttg caacctctaa ttattttaac ggatttaatt
     1321 ggaggaacac ctaataatgt ttctattaaa cagttgaaca acaatagaat tcaaattatt
     1381 gctgggatta atttacctct tttactggag ttattaatgg cccagtcagc tggtaaatct
     1441 atagaagaaa tagattttaa ggaattactg aataactctc aaaaaa
//
LOCUS       DAHUWM010000334         1486 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.341, whole genome shotgun sequence.
ACCESSION   DAHUWM010000334 DAHUWM010000000
VERSION     DAHUWM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.341"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..210
                     /locus_tag="NK788_002834"
     CDS             <1..210
                     /locus_tag="NK788_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCH6771117.1"
                     /translation="ARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRELFKDFLLFV
                     TITSQLQHAHQANKREAESFSWETI"
     gene            230..1450
                     /locus_tag="NK788_002835"
     CDS             230..1450
                     /locus_tag="NK788_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCH6771118.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
BASE COUNT          514 a          268 c          300 g          404 t
ORIGIN      
        1 gcacgaatta tgccaagctc agaattttta ttgggtcatt cactccaagc ctttgatgag
       61 caaggacgac tcattgatga agaacaagtc gcaaaattac gtgaactatt caaagatttc
      121 ttactctttg tcacaatcac tagtcaattg caacatgcgc accaagcaaa taaacgtgaa
      181 gccgaaagct tttcatggga aacgatttaa tcgaggggag caaacaaaga tgaaattaat
      241 aggtatcgtc ggatcagcag ctgaactttc atataaccgt aaattattaa catttatcca
      301 aaaacaatat catgaactat ttaatttaga aatcattgaa atcaaagatt tacctctgtt
      361 caatcaagat gatgatcaaa caaatagtcc tgctattcaa cagctaaatc gcaaaatctt
      421 aatggctgat ggggtaatta ttgctacgcc agaacacaat catacggttc cagcaggatt
      481 gaaaagtgca cttgaatggc tatcatttaa aatccatcca cttgaaaata aacctgtaat
      541 ggttgtgggt gcttcttact acgatcaagg aacttcacgc gcgcaattac acttgcgcca
      601 aattttggac gcaccaggcg tgaatgcatt agtgatgccc ggaaatgaat ttttattggg
      661 aaaagcaaaa gaagcatttg accaagaaga tcgcttgatt gatccacgaa ctgttgactt
      721 tttaggcagt acattagcaa gttttgtaaa attcattaaa gtcgtgtcta ccttagcagg
      781 gcctaaaaag cttccaccag aagatttgca tgcgacagga acaattgcta cgacaatacc
      841 cgatgttgat atgtcagatg atcattggtt ggaaaaagct tctgaagcag tcggtgccgt
      901 ttctggaaat acttacgtca aattaaatcg tgggttattg acagtggatc aaatcaatta
      961 ctttttagat tcgatgccga tggaattaac ttttgcagat aataataacc aatttttata
     1021 ttataatttg cgccaagaag cgaaagagat gttagctgct cgtgtgcccg gtcaggtagg
     1081 gaatccatta gccaactgtc acccagaaaa atcgtataaa aatgtagagt gggtaattca
     1141 acaactgcga agcggagcaa cagatgcggt acgtgttcat gtaccaaaac aagtgccaga
     1201 aaaatatatt gtgcataatt accaagcaat gcatgatgaa aatggagagt atgctggaat
     1261 caatgaatac attcttgatt tcaaaccaat cattgagtgg tatttacaac aaacgggtca
     1321 acaacttgtt ggaaatgaag atgcggtttc aagtgcatct gttcatggtg gaaaaacaga
     1381 tgcagtaacc agcgccagca cacacgatga aactgcagta gatgcagttt cgagtgcttc
     1441 tgtaaaataa aaaggttctg ttgcaaagtt ttaaataaag aataaa
//
LOCUS       DAHUWM010000335         1484 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHUWM010000335 DAHUWM010000000
VERSION     DAHUWM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.342"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            66..1235
                     /locus_tag="NK788_002836"
     CDS             66..1235
                     /locus_tag="NK788_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCH6771119.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          538 a          218 c          234 g          494 t
ORIGIN      
        1 aaaaagaaag tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact
       61 ttctcatgcc caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag
      121 atgaattatt tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa
      181 cagcttttct tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta
      241 atgggaatta ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta
      301 gagatagaaa tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact
      361 ctttagaaac cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg
      421 aattgattga aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta
      481 aaatagtttc tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg
      541 tcatttttat ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag
      601 tttatattgt aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg
      661 ctccaactga atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt
      721 taaaagaggt tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata
      781 gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc
      841 ataaggttcg tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag
      901 caagttcaaa agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc
      961 agtatccacg agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc
     1021 ctccgtcaat cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat
     1081 taaagaaata taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc
     1141 tagtatctca attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta
     1201 aagaaataca tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttcc
     1261 atttacacaa aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat
     1321 attaataaaa atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt
     1381 atttcacttt atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat
     1441 ttaattatgt aaattagcta ttctaagaac ttctacggat aact
//
LOCUS       DAHUWM010000336         1481 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.343, whole genome shotgun sequence.
ACCESSION   DAHUWM010000336 DAHUWM010000000
VERSION     DAHUWM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.343"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            336..1439
                     /locus_tag="NK788_002837"
     CDS             336..1439
                     /locus_tag="NK788_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCH6771120.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          479 a          241 c          338 g          423 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc c
//
LOCUS       DAHUWM010000337         1468 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.344, whole genome shotgun sequence.
ACCESSION   DAHUWM010000337 DAHUWM010000000
VERSION     DAHUWM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.344"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            117..1076
                     /locus_tag="NK788_002838"
     CDS             117..1076
                     /locus_tag="NK788_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6771121.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1083..1468
                     /locus_tag="NK788_002839"
                     /pseudo
     CDS             1083..1468
                     /locus_tag="NK788_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          538 a          249 c          267 g          414 t
ORIGIN      
        1 attatatcac aatttattaa aacgtcgatt gtaaaattaa gctagacaac taaaaagatc
       61 atttgtgcta cactcaaaat agttccgacc aaagaattaa taaggagtga gtacaaatga
      121 cttacaccca tcttacaaca gacgaactag taatcataga gtcttatttc aatatcgaga
      181 aacctgtcgc tcttgtcgca caaagtttaa atcgctctag acaaaccatt tataatgttt
      241 attcgtttct caaacaagga aaaaaagtgc tagattatta ccgtcaatac aaacaaaata
      301 aatctcgttg tggacgacgt tcaaccgttt tatctgatga acaatcaaca tatgttcaag
      361 aaaaggtagc tcaaggatgg tcacccgatg tgattatcgg ccgtggcgag tttccaattg
      421 attgttctgt tcgtacactc tatcggatgt ttaaaaaagg cctttttgac acacagtcgt
      481 tacctatgaa ggggaaacgt aaaccgaata atcatcaaga aaagcgtgga aaacaggcct
      541 ttcgcagaac gattcatgaa cggaacagtc tctatccaca cctgactaaa gaatttggtc
      601 atctcgaagt cgacaccatt gtgggagtta aacacaagag cgctgttatt acattggttg
      661 agtgtttatc caaggttatt attactttga aaccgacagg cagacgagcc gaagatatcg
      721 aaaacaggtt aaatgattgg ttcaaatcca tgcctaaaca cttgtttaaa tccatgacat
      781 ttgattgtgg aaaagaattt tccaactggc gatctataag taactcgaac gatattgcca
      841 tctttttcgc agatccagga acaccttcgc aaagagggct aaatgagcac tcaaacggct
      901 tgcttagacg agatggctta cctaaaacaa tggactttag ggacacggat gaaaccttta
      961 ttcaatccgt tgcctcgaaa agaaaccata ttccaagaaa atcattaaat tatcgaacac
     1021 cattggaagt atttttgagc tgcatagata aagacatatt gtctagctta atttgacaaa
     1081 tcagaaatat taataaaaca gtggaaaaga atggagttta taaaggtgat tttcattttc
     1141 aaaatgctga gactattgaa cgagaacatc attatttcga tttattagtg aatacagtgg
     1201 tagaattagc gaaatgggca aagtcaaacg aaacttatag tcaagtactt aaaagaatta
     1261 tgagtcaaga aacttcattt atctatgagt taagcaagtc aaatttgaaa tatacttata
     1321 atgaagatga acttgaaatt ttagataatc cagaaaattc acaatggatt aaagagttga
     1381 agagagaaga ctttattgag acactaagaa ttctaaaatc gttatttgat ttgaaattag
     1441 ccaaagctac catcatggga attgaaag
//
LOCUS       DAHUWM010000338         1461 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.345, whole genome shotgun sequence.
ACCESSION   DAHUWM010000338 DAHUWM010000000
VERSION     DAHUWM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.345"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..660
                     /locus_tag="NK788_002840"
     CDS             <1..660
                     /locus_tag="NK788_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCH6771122.1"
                     /translation="TTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDSY
                     GRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMI
                     YQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVF
                     SKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDN
                     E"
     gene            838..1179
                     /locus_tag="NK788_002841"
     CDS             838..1179
                     /locus_tag="NK788_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771123.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1172..>1461
                     /locus_tag="NK788_002842"
     CDS             1172..>1461
                     /locus_tag="NK788_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCH6771124.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          545 a          219 c          308 g          389 t
ORIGIN      
        1 actacgagct tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat
       61 attgatgcac taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa
      121 ccagattcat atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg
      181 ctggctatgg ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca
      241 ttcaacgaaa tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg
      301 atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat
      361 actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt
      421 atgggagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc
      481 tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct
      541 cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc
      601 cgtatggaaa ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag
      661 agctgaagcg ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc
      721 aatcatccta agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg
      781 actccgccgg caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg
      841 gaaaaagaac tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa
      901 attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct
      961 tgggatcaag atgatttagg caaccatgta actatttggg atcatgttta tcttatccat
     1021 ctatcgaagc attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat
     1081 gcgctagaag agtacgacag agataacttg attgatatct cttattacgg accaggtaag
     1141 aaatacattg ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt
     1201 tgaaaaactt tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta
     1261 tttatcttgg gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac
     1321 tattaatgag tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga
     1381 ggaatataaa ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt
     1441 taaaggacac acggaaaccg a
//
LOCUS       DAHUWM010000339         1448 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.346, whole genome shotgun sequence.
ACCESSION   DAHUWM010000339 DAHUWM010000000
VERSION     DAHUWM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.346"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            5..403
                     /locus_tag="NK788_002843"
     CDS             5..403
                     /locus_tag="NK788_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCH6771125.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="NK788_002844"
     CDS             387..1082
                     /locus_tag="NK788_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCH6771126.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          546 a          209 c          295 g          398 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaa
//
LOCUS       DAHUWM010000340         1446 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.347, whole genome shotgun sequence.
ACCESSION   DAHUWM010000340 DAHUWM010000000
VERSION     DAHUWM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.347"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..777
                     /locus_tag="NK788_002845"
     CDS             <1..777
                     /locus_tag="NK788_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCH6771127.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            788..1446
                     /locus_tag="NK788_002846"
                     /pseudo
     CDS             788..1446
                     /locus_tag="NK788_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
BASE COUNT          502 a          218 c          276 g          450 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      781 ttaagataag ttaatgtgta gtaaagaatt tcagcaaaaa cgtttagcac tgattttggc
      841 tcatggagat ctaacggcaa cctgtatagc aagtactgct aataggatcg ttgagagata
      901 tatatacgac gccattgata tgccactaat gacttcaagt agtgagattg cagacaaaat
      961 taatagatac ttacaagagt gtaccggata tgaagaaatt attttacttg ttgatatggg
     1021 ttcactggag aaaatttatc taaatttgga actagacgct caaaaaaaat tcggactact
     1081 aaacaatgtt tcaacaagaa tggctattga tgttgcaaat cagatattat tagatttacc
     1141 gcttaaacag attcttgaac aagtaaaaga aaatcaaaat gtggattata tttatgaatc
     1201 atcaaatatt aagaaggata tgattgtctg ttcttgtgca agcggattag gaacttcgtt
     1261 taaattaaaa aagatacttg aagatagctt tccaagggat actgatcttg tcatagaaac
     1321 gtgtgactat tactctttag tgaatgaaaa ctatttatcg gagcttcgta aagagaacaa
     1381 tattcttttt attgttggaa cactaaatcc acatctagaa gatgtagaat tcttttcaat
     1441 tgaaga
//
LOCUS       DAHUWM010000341         1440 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.348, whole genome shotgun sequence.
ACCESSION   DAHUWM010000341 DAHUWM010000000
VERSION     DAHUWM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.348"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            559..780
                     /locus_tag="NK788_002847"
     CDS             559..780
                     /locus_tag="NK788_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771128.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            781..1038
                     /locus_tag="NK788_002848"
     CDS             781..1038
                     /locus_tag="NK788_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771129.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1174..>1440)
                     /locus_tag="NK788_002849"
     CDS             complement(1174..>1440)
                     /locus_tag="NK788_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCH6771130.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          495 a          323 c          232 g          390 t
ORIGIN      
        1 gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg cattactgat
       61 tttgtcaaaa aaaaacgctg aaacccttgg gagagtaagg ctaaacagct atcgaccaat
      121 gaccaatgac caataagggg ggtgtcgtag tccccccctc ctccgcccaa ttcataaaca
      181 ccaaaaccaa aaaacaaacc tcagggctcc gccccgaacc ccgacctcct tctaccgaaa
      241 tatatgcagg cgccacgctt actatttcgt tcagctatcg gcttttgcaa tctaacacga
      301 ccgtttcgct accagtcgcc ttacaggctc cattctcgct tctcttacgt gttttaacga
      361 ttgcgccgat aactaaacga aatagacgct aaatcgctta aaacgcaaaa tagacccttt
      421 ttagagcatt gattttttct actagtcatt tcgataatat aaaattgacc acaacacaga
      481 aaacttatat tataagctta tgtcgtaagt ccgacgtact cacgtaatat agacggacgt
      541 taaatggaag gagcataaat gagagtgata aaatcagaaa aattcacttt ggccgaaatt
      601 atgacgacca gcgaacttgc acaagaactt gattacaacc aagcttacgt tttgcgctta
      661 gcgaaagaac atctgactga aggaaaagaa tatcgctctg ccggtagaag aaactatctt
      721 ttcagcccag aagcccttaa aaaactagaa aaagtcataa ccaccaaaga aggtgagtaa
      781 atgcctaaag aaacaactat caacgttgaa caactagaaa aattgctaga atcatattcc
      841 aatgatttta tcgaattgac cggcggaaaa gtcgaagaaa ccaccgacct ctttgaccta
      901 cttctcacaa atattctgga attaaactct gaagaacaaa aagagcataa gggaaaactg
      961 atagcacctt cctacaggaa ggtggctaag tcggctgtgc caactggttt tcttacaaaa
     1021 caaccttata ttttttgata tcacaaaaaa atccattttg gacctatttt cggtcataat
     1081 atagtaccta cttttggtca tagtttcgtc cgtagttttg gtcatagtga ctaagacttt
     1141 cacgcacaaa aaaagagctg aaattaacag ctcttatcct ttatttattt ttgaatttac
     1201 acttccacca tgacgattcg attttatata ttgcccgata atataagctg aattatcaca
     1261 aagaagcgga aaccaatcat caaatctttc tgacattgca tagtcaatta aatctttgat
     1321 ttcagtgata ttcttagaat caacaaacga catcatctct ctaatcaact catatcgttc
     1381 tgcactcgtt actgacaaat aactagcaat ttctgcacca ccatgagcga taatatcact
//
LOCUS       DAHUWM010000342         1429 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.349, whole genome shotgun sequence.
ACCESSION   DAHUWM010000342 DAHUWM010000000
VERSION     DAHUWM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.349"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1350
                     /locus_tag="NK788_002850"
     CDS             <1..1350
                     /locus_tag="NK788_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCH6771131.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          563 a          225 c          309 g          332 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaata
//
LOCUS       DAHUWM010000343         1425 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.350, whole genome shotgun sequence.
ACCESSION   DAHUWM010000343 DAHUWM010000000
VERSION     DAHUWM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.350"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            100..1392
                     /locus_tag="NK788_002851"
     CDS             100..1392
                     /locus_tag="NK788_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6771132.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          461 a          294 c          260 g          410 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa tgccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cactgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttgttgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat cattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DAHUWM010000344         1418 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.351, whole genome shotgun sequence.
ACCESSION   DAHUWM010000344 DAHUWM010000000
VERSION     DAHUWM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.351"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(150..1337)
                     /locus_tag="NK788_002852"
     CDS             complement(150..1337)
                     /locus_tag="NK788_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCH6771133.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DAHUWM010000345         1404 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.352, whole genome shotgun sequence.
ACCESSION   DAHUWM010000345 DAHUWM010000000
VERSION     DAHUWM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.352"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(27..1058)
                     /locus_tag="NK788_002853"
     CDS             complement(27..1058)
                     /locus_tag="NK788_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6771134.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          424 a          231 c          240 g          509 t
ORIGIN      
        1 tgtgaaaaaa agatcataca tttgttttat atggaagaaa cccgaacgaa tgccgctaat
       61 tcgtgataag aaaaatcaac catcttttcc cagatatctt gaaaagaatg ggatgagagg
      121 tatcgattac cacataaata atttaccgcg tcagcatcat ctaaatatag ttctgcataa
      181 taacgatatg tttttctgca tagttctggc gatacgtttt tatatactgt aattttgctc
      241 aaaatttggc gaatagtccg aggagaaatt tgctgatcat gtgaggaacg aaaaacaaaa
      301 gaattttcgt ccgtattttg tgctgttaac ttaaaatagt tctccagaaa tggcatcgct
      361 tcttttggta ttggtacttt attcagtctg ttttcttttc tattcagatt aagaatctgc
      421 ttggtcaaag atacatcttt acgtttaagt tctactactt catggatctt taatccgcaa
      481 tacagaagaa tggcagtaat tgcaaggttc ctcggtctat tcttttcttc aaagctagct
      541 tctctgcttg tttgaaagcc ttcaatcggg ataaacaaat gctcaaaatc tttgaaaagt
      601 tctgcatcga tatatcgcgg agctgctttg gttgtttccg gtaaacttgt acccaaaaga
      661 gaaatctctt cttcaatcag tcctttttga tacaaaaatc gtaaaaaatg atttaatgaa
      721 tattgcttcc ttgaaatccc gtaatagcta ttttcaaaca attgctccgt ttcttggttc
      781 gctgtagttt ggaacgaacg agtatgtcga tagattttag ctaagaaatt ctgataatct
      841 tgtttttcta aatggataat cagctgatca tccacttctt cagaaaatac ttttctcaaa
      901 taatcagtaa acagatgaaa atcttttgcg tattcatagc gactgcctaa tgaaagggaa
      961 gaacgatttt cttctaaaaa ctgttgaaag acggaaggga gcaagtgtgt acgttgcttt
     1021 aacttttctt gcagttgttt tctagttttg ctgtccatta caaattccct ccaatagtgt
     1081 tcattttcta tattttaccg catttcatca aatgaagata gacagtttgt cttgatctta
     1141 ggaagcaaat ttttaactaa gtactagcta aaagatatga agtaagaaga tacattttta
     1201 gttattttat tagtgcagtc attcttttga tcttttaatt gatttatcct ctttttagct
     1261 atgtggttac ttcatgagca atgaaatgga tttggttcgt tctaatggca ggaggctctt
     1321 tcttttattt ccgaaaaatt agaaaacatt ttttcagaat cgattggctc tccatttatt
     1381 caataatggt ttgtctaacg atat
//
LOCUS       DAHUWM010000346         1395 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.353, whole genome shotgun sequence.
ACCESSION   DAHUWM010000346 DAHUWM010000000
VERSION     DAHUWM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.353"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            329..1369
                     /locus_tag="NK788_002854"
     CDS             329..1369
                     /locus_tag="NK788_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCH6771135.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          545 a          188 c          226 g          436 t
ORIGIN      
        1 tactagtaat ggtagtatgt tttatatgca taaggtagtt cttaatttat tttttgtttt
       61 gacactggta gtacttaatt atctttaatt ttaaaactaa attttattca tacgtttttc
      121 tggtagtatt taattataaa taatatttat ttaaagtagt tcttaatgac tttaaaggta
      181 gtacataatt cattttattt ctgattttag gtagtattta attaaaaaac agttttaatg
      241 cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat
      301 aaaacaacgt gaaagtagag gatctacaat gactgatttt aaatttttta aggcggatag
      361 agtttacaac gaattatttt atcaatttcc aaaagtcttt attgtttctg acgaatacaa
      421 aaaaatgaaa gattcaacta agattgccta tatgctttta aaagcaagat tagagatcgc
      481 aatcagcaaa cggcaaatcg atgaagaagg taatgtttat tttacttata cgacaaatga
      541 actatgtaga gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga
      601 atcctttggt ttattattac aaaagcagat gggatttaac aaacagttag ggaaaaataa
      661 tcctaataga ctatatctag cagaattaaa agtctcagaa aatgatatct acttactcga
      721 aaaatttgat agagagaata gggaaaacgt tgataaatca gagggtatga aaatcatacc
      781 caccctcgac gaaaaatcag acgctgaatc ccttggggct caagagggta tgaaaatcat
      841 accgtgccaa aacgttgata aatcagaggg tatgaaaatc ataccagaac ttaataataa
      901 tatattagac actaatagac acaatataga cactgaaaaa gaccgcctac aagatcaatt
      961 gttgttagac aattttgaga caattatgac aaacgacagc attgctacgt ttgtccctga
     1021 acgagtatta aatttgataa aaacattttc ttcaagttac agtgaagctc aaaaaaccgt
     1081 ccagactatt cataatgcaa agaaaaaagc tgaaatagaa agtggtattt cgatagtttt
     1141 tgaagaactc gatagttatt atgtcaatgc agaacaagaa ttatacacga cactgttaaa
     1201 agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta
     1261 tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt
     1321 gaattatgaa agctccccaa gcactattac gaaagactgg ttagagtgaa actttactga
     1381 caaagtgatt gttgc
//
LOCUS       DAHUWM010000347         1382 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.354, whole genome shotgun sequence.
ACCESSION   DAHUWM010000347 DAHUWM010000000
VERSION     DAHUWM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.354"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            474..725
                     /locus_tag="NK788_002855"
     CDS             474..725
                     /locus_tag="NK788_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771136.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="NK788_002856"
     CDS             759..1037
                     /locus_tag="NK788_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771137.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          490 a          192 c          260 g          440 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 at
//
LOCUS       DAHUWM010000348         1381 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.355, whole genome shotgun sequence.
ACCESSION   DAHUWM010000348 DAHUWM010000000
VERSION     DAHUWM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.355"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(353..967)
                     /locus_tag="NK788_002857"
     CDS             complement(353..967)
                     /locus_tag="NK788_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCH6771138.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          447 a          269 c          231 g          434 t
ORIGIN      
        1 aatgtactat aattactttg taaagagttt tggggaagac tctttcatca acgtgtatta
       61 agagacaaga aagaatccat atggattctt tcttgtctct ttttccacta ctttttattt
      121 ttctgacact acatattctt ataactcatt tctcataact gtgaaacaac ttataaaaat
      181 aacaaacttt aaacaaaaca agcttaaaat caaagatgat ccatcttaaa ataactaaaa
      241 caaaaaaagt agtcgttgtt aatcctcatt aataatcgtt ttcttttttt caaacgctcc
      301 atttttatgt ggaacattat ttttttaagg aactttgtca acatgtggat aactattttc
      361 cacttgtgtc ctggactagc tgttttgctg taaaatacaa aacttgctgc agcaaaaaga
      421 tcgttgcaag agaggctgtt ccgcagatcg ctggatctag atttggcaaa gtcaagactg
      481 catcatgtaa attggcgtgg ttgtccaaaa ccaaatcagc aaattcaaat aatcgcaaac
      541 cactgctatg ttgcgatttg atacttcgtg acaaatcaaa tccagtaaca atgattaatg
      601 gatgaccatt ttctttgatc cactgagcta gttcgatgat tgccgggttt cttccttcat
      661 tcgaaagaag aagaaaaatg tcttctgatt tgacagaaag ctgatcaata attgcctgtc
      721 ctgcaccttc tagcgtttca tagacacctc cggctggatt ttcaatcaat atacttggta
      781 caagatccgg ggtttgatcc caaaattcaa atgctacacc acttagtgtg cggctagcat
      841 atatctgcaa tctaccacct tgattaattt gtttcgcgat ccaagtgcca gcttgaagca
      901 tcagcggctc ttctatcctt aatttctctt ccaattggtg ataatagtca aaataacata
      961 aactcattta tctctcccgc ctagctatac ttgacatgaa tagaaacagc tgcctaatct
     1021 aaggaagtac ataaaaaaac agtagttaga cagtaacaca tgtggtcttt gctgtttcaa
     1081 acacaaaaaa ggcattgaac gaatacgccc aataccttga catagcaaat aaaacaacgt
     1141 gcagaccatg gaaaatatct acataggggt agaattttca ccctagatta ggtacaataa
     1201 attaggtcga tgcactgcat tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga
     1261 ctggtcattc cctgattctt agcgccaact aagggtcagg gagccttttt ttattcagct
     1321 gtttctatct gaatttattg taagtaatct cttatccaag tgcaagcaag aaaatacaaa
     1381 a
//
LOCUS       DAHUWM010000349         1369 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.356, whole genome shotgun sequence.
ACCESSION   DAHUWM010000349 DAHUWM010000000
VERSION     DAHUWM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.356"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            398..1309
                     /locus_tag="NK788_002858"
     CDS             398..1309
                     /locus_tag="NK788_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCH6771139.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          544 a          201 c          260 g          364 t
ORIGIN      
        1 ttttggtagt tttaatatat tcatttttag ttgtataaaa atatatcata ataaaaacaa
       61 caaaaaaatg tctggaaaat cgaacataaa agtgtctgat ttgaatatcc cagcagcgga
      121 agaagaaaaa caaaatcgat aacatttgtt ctaagaatta gttacgttaa atacggaacg
      181 caagatagac ttgaaaaacg gtaacaaaag aagtagtata ttaaggtgtg atgtgctact
      241 tgacatttat tctgctgagc gaactttctg gattgtgtag ccttatatct attcaggacg
      301 tatcgagcag attagtaaag gtgttcttga tgattttcat cttgaacacc tttgttttta
      361 gcaattcgca aaaaacgaaa agtaggagaa ttgataaatg aaaaaaagat ttttagcact
      421 tgccatcgta ctaggcactg gcttattgtc aggctgtacg aatgccggtg aaaaaacagc
      481 cgttagttat aaaggcggta caatcagcga acaagaagta atggatagct tgaaaaaaat
      541 gcaaggagca gattccgctg tgcaacagtt gatcgtttat caagtatttg aagataaata
      601 tggagatgac gtatcaacca aagaaatcga ttctcaatat gatcaaacaa agaaacaatt
      661 gggtgattca tttgattcac aattgaaatc tgcaggttac acagaacaaa cattcaaaga
      721 cagtatcaaa caaagtctag cctttcaaga aggtctaaag aaacatatca aattgaccga
      781 tgaagattta aaaacggctt gggaatcttt ccatccagaa gtggaagctc aaattatcca
      841 agtagcaagt gaagatgatg caaaagatgt gaagaaagct gctgataagg gagacgattt
      901 ctctaaatta gccaaagaca agtcaactga tactacgaca aaagaagatg gcggaaaagt
      961 gaaatttgat tccactacta caacggttcc ggcagaagtc aaagaagcag cctttaagtt
     1021 gaaagatggt caagtttctg atgtgatcac ttctaccaat gcttcaactt atactacgga
     1081 atattatgtg gttaaaatgg tgaaaaacca aaacaaaggc aacgacatgg ataaatacaa
     1141 aaaagaattg aaagaaattg caacagacac aaaattaagt gacagtacct tccagaataa
     1201 agtaattggc gaagtattga aagacgctaa tgtaaaaatc aaagacaaag actttgaaaa
     1261 tgttttgagt acattcactt cagattctag tacagcaagt agtaaataag cggtataaat
     1321 aaaaaatatg aactatttaa atcaataaaa ctgttcatct aaatctttt
//
LOCUS       DAHUWM010000350         1359 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.357, whole genome shotgun sequence.
ACCESSION   DAHUWM010000350 DAHUWM010000000
VERSION     DAHUWM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.357"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            286..>1359
                     /locus_tag="NK788_002859"
     CDS             286..>1359
                     /locus_tag="NK788_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCH6771140.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMER"
BASE COUNT          429 a          260 c          289 g          381 t
ORIGIN      
        1 taaaaataaa agtgcaataa aagtgcaatt tttgcaccta atcatactct gtttatatat
       61 gaaaactaga ttctgaatcc aatatttggt taacgagagg cgttcgaatt tgaacgtctt
      121 ttatatatta ctttcaaata aatatgaggt agaatactat ctattttctt tttaacccaa
      181 ttgttataat ggagagtgta aaatattttg tgtaaataga aaaaaggaag tcccttctgt
      241 agaatagagt taccacaaca cattcacaga agagaggact tccctatgaa cgattttact
      301 acagaaattc taaagactct agcgaacaaa ggcgatttga atgaattatt ccgtgtccat
      361 ttggagaaag cagtcaatac gcttctcaaa acggagttaa cggctttcct agattacgaa
      421 aagtatgatc gcattggttt taacacgggt aattctcgta acggctccta tgaccgtacg
      481 gtcaagaccg agtatgggga acttcatctc cagattccgc gcgaccgcaa tggtgagttc
      541 aagcaacaga ctgttcctgc ttatagacgg acgaatgaca cgttagagga gaccgtcatt
      601 cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag acttgattga gaaaatgtat
      661 gggcattact acacgcccca aaccatgtcc aatataacaa aatcatttac agaagaggta
      721 acggcgttta atgggcggga gcttcatgac cgttatgctg ctatttatat ggacgcaacg
      781 tatattccgt taaagcggaa aaccgtcgcc aaggaagcta ttcatatcgc agttggcatt
      841 cgcccggacg gatcaaagga agtattgagc tatgcgattg caccgactga atccatcacg
      901 atttgggagg aaattttatt ggaccttcaa gagcgcggtt tgaaaaatgt cctcctgttc
      961 atcacggatg gcttaaaggg gatggtagga gcgattagtc ggttctatcc caaagctcgt
     1021 tttcaacatt gttgtgtaca cgtttcccgt aatatcagtc ataaagtgcg tgtcgatgat
     1081 cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag cctcatctaa agaggtggcg
     1141 ttggaagcac gtggtacttt tgcggaaaaa tggaaaacca gctatccaaa agtggttgaa
     1201 tcgattcttt cgaacgatca cttgctcact ttctatgatt tccccttggc catacgcaag
     1261 agtatttatt ctacgaactt gattgaatcc tttaataaac aaatcaagaa atacagccac
     1321 cgcaaggaac agttccaaaa tgaagagtcg atggaacgt
//
LOCUS       DAHUWM010000351         1318 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.360, whole genome shotgun sequence.
ACCESSION   DAHUWM010000351 DAHUWM010000000
VERSION     DAHUWM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.360"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            38..1150
                     /locus_tag="NK788_002860"
     CDS             38..1150
                     /locus_tag="NK788_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCH6771141.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          463 a          245 c          219 g          391 t
ORIGIN      
        1 taaaaaaaga aaatattcat ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt
       61 ctctacagga actattcttg gatggcaatt ttacaataat gaacactctc agatagttgc
      121 ccatgctcaa tcagctcaaa aaaagttgat actgactacc aatgaacaac agctcgttga
      181 aaaaatcgat caactattaa gaaaaaatga ttatattggc tcaatttacg taaagaaaaa
      241 taatcgcatc atcatggaaa aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa
      301 tgatccttct ctttattatc agattggttc ggttcaaaaa gcattgacta ctcttttgat
      361 tttaaaacaa gtcgagcgtg ggcacctttc tttagatact aagctggctc aattctatcc
      421 acaaattgct ggcagccagc aaattactat ccgtgacttg ctgtatatgc gctcaggctt
      481 gcaccgaact gcttctccaa ccataccgat gactgatgaa gaagtcgttc aatttgcgat
      541 tcagcactta aaatttacag attatcagac ctctcattat gattcattga actttaccat
      601 attgactgga attttgatca aattaacaaa tcaaccgtat gaaaaattat tgaccgatga
      661 tatcatcgaa cctttaggat tacagcatac aaaattttat gaccaagtca aaaattcatc
      721 caatcatgca ctatcctatc aaatgtctaa agaggatgat tactttaaac cactgacaga
      781 atctgaaaca gagatccata acgaattagg gactggtaat atcagtatgt ccgtctatga
      841 tttaaatacg ctgtttacac aagttttatc tggaaaacta gtttccaaag acttactctt
      901 ctcattatgg cgtgaaaatg cgaatggaca tccatatagt ggtggggtat atagcggaga
      961 taattatatc ctgtcgcaag gaaatatcga ctcttttgat acatctgcta tatttagcaa
     1021 agattcacag gatgctatca tcatggaaag caatataaaa gctgacaaaa ctatcaaact
     1081 gcctactgct gatctaagaa accaaatcta tgacctgatg gaaggaacta ctaagttggc
     1141 aaatagctga tatgatgaaa agatagcaaa tactacttct ttgaatagca tttgcttaaa
     1201 aaaagtactc gactaaacgt aggaaagagt gtgggacaaa tctaaaaatc aatttttgct
     1261 acagacttta attccaaata aacagaacag aaagaggtgt tcttctttct gttctgtc
//
LOCUS       DAHUWM010000352         1314 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.361, whole genome shotgun sequence.
ACCESSION   DAHUWM010000352 DAHUWM010000000
VERSION     DAHUWM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.361"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..848
                     /locus_tag="NK788_002861"
     CDS             <1..848
                     /locus_tag="NK788_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCH6771142.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DAHUWM010000353         1295 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.362, whole genome shotgun sequence.
ACCESSION   DAHUWM010000353 DAHUWM010000000
VERSION     DAHUWM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.362"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..626)
                     /locus_tag="NK788_002862"
     CDS             complement(<1..626)
                     /locus_tag="NK788_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771143.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1295
                     /locus_tag="NK788_002863"
     CDS             914..>1295
                     /locus_tag="NK788_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCH6771144.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYK"
BASE COUNT          432 a          201 c          248 g          414 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taagg
//
LOCUS       DAHUWM010000354         1294 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.363, whole genome shotgun sequence.
ACCESSION   DAHUWM010000354 DAHUWM010000000
VERSION     DAHUWM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.363"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1244
                     /locus_tag="NK788_002864"
     CDS             <1..1244
                     /locus_tag="NK788_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCH6771145.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAHUWM010000355         1292 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.364, whole genome shotgun sequence.
ACCESSION   DAHUWM010000355 DAHUWM010000000
VERSION     DAHUWM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.364"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            465..704
                     /locus_tag="NK788_002865"
     CDS             465..704
                     /locus_tag="NK788_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771146.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1066..1290)
                     /locus_tag="NK788_002866"
     CDS             complement(1066..1290)
                     /locus_tag="NK788_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771147.1"
                     /translation="MCQPIGLKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGK
                     VVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          430 a          241 c          179 g          442 t
ORIGIN      
        1 aaactgattg aaataacccc tccgtaaaaa atagtctgtg aatataccca ctttttttca
       61 gaagacgtac ttcgtttggt tctaaaacct atccctgcat ttctactctt atcaaaaaac
      121 caaatattta gaagtaataa tattgctatt attaagataa aattactgta aaaatccaaa
      181 agctatcctc ctagcaacta tttaaatacc caaaaaaatt acctctagat aatatcatac
      241 ctacgttttc acttataaat tttttcataa aaaaagcgtt tagaggcata atcgttaata
      301 cagatggtaa ccaatgtatc tggtttgaac tgggatatca ccctctccat atatgtatta
      361 ctaagactaa tagatactat aaaagacttg atagttttgc attgattttg cctaatactg
      421 agcataggtg tatgctaaac gatactataa ggagtgtgta cataatgaaa agaattctgt
      481 ttatcagttt atcaaattta ttgttactta ctgcttgtgg gacttctgca tcaaagaaca
      541 taccttcaga tacgcaaatt tcgaatagta tcgattcaac aatttctaca aatgagaaaa
      601 attctccggt agaaacttcc gatataaaag tttctacctc tgcaaaaaac agggtaaaga
      661 aactgtcagc acgtctcctt tatttcaaac aaactctgaa ttagcttctt catcttcttc
      721 agcaaataat gatgatagtt tcgcgtttac tcctaactaa atgcaacctt tacaaatgct
      781 ttacgataac aagcctgagc taaatgatcc tgatatcagt tttgcatttt ttgaaatgat
      841 tggatcggat gatcttttta aaactactag tttgtctatc cgaaaatagg acagttctgg
      901 cagtataggt ttttatcgag tttctccacc aggcctcgtt acagagacag attctctagg
      961 cgaacctttt gaataaatca aaaatcatga acaatcactg ccaactaaat aaagtgcctt
     1021 caaaagcttg gtcttctcgc ttttgaaggc actttattta aatctttatg aaactaaagt
     1081 atgttcatat ttgttgctat agttatatag atattctgtt gtttccaact cataaacgtg
     1141 agtatatttt gccactactt ttccgcattt ttctaatatt tcattgcgat cgacgtcatg
     1201 atattcgtct acgcagatca caacacaatt ttctaattta ctgattactt ctccaaccac
     1261 tttttgtttc aagccaatag gttggcaaat at
//
LOCUS       DAHUWM010000356         1234 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.366, whole genome shotgun sequence.
ACCESSION   DAHUWM010000356 DAHUWM010000000
VERSION     DAHUWM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.366"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1126
                     /locus_tag="NK788_002867"
     CDS             join(<1..240,240..1126)
                     /locus_tag="NK788_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6771148.1"
                     /translation="KQQIVDLYLAGKPRAEIIREYELTPSSFDKWMKQAQSTGSFKER
                     DNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANKHKYSISAMCKILN
                     ISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRIGRI
                     MKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWH
                     YICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIH
                     EILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWW
                     NYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          403 a          241 c          272 g          318 t
ORIGIN      
        1 tcaaacaaca aatcgtcgat ctctatctcg ctggtaagcc tcgcgcagaa attattcgag
       61 agtatgagct tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct
      121 cattcaaaga aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga
      181 aaaataagca actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac
      241 gaaaagacaa gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat
      301 tctaaatatt tctcgtcaga cctactatta tcaagcgaaa ccgatcgaaa atgagtccga
      361 cttagaagag atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccgaaa
      421 attgaagaag tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa tcggtcgaat
      481 catgaaacgc cgcggattga catctaccta tacgatcgct cattttaaag ggcaacgaac
      541 agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg acctttacac aagaacagcc
      601 attggaagcc atcgttacgg atcttactta tgttcgcgtg gggaaaaagt ggcattatat
      661 ctgcttaata cttgatttgt ttaatcgaga aattattggt tattcctgtg gtgagaagaa
      721 agatgcctca ttggtaaaag aagcctttgg acggataccg tattctttaa cagacgtcaa
      781 gctttttcat acagaccggg gaaaggaatt tgataaccaa accattcatg agattctgaa
      841 tggttttgga attactcgtt cattgagtag gaagggttgt ccgtatgata atgccgttgt
      901 ggaatcaacc tataaatctg tcaaagtaga attcgtgcat caataccaat ttgagacact
      961 ggcacagcta cgtctagaat tgtttgatta tgtgcattgg tggaactatc tacgcttaca
     1021 tggcacgttg gcgtatgaaa caccgatcca aattcgacaa cagagattgg cgaagcgaat
     1081 ccttgataat gagcgcggat ctgatacctc tggagaggca gcgtaattga atgattgtgc
     1141 ttctgccgga gaaaatcaga tccgaggatg ctcattgtca agggcaatcg tagccataac
     1201 accgcagcat tcacaacacc ttataatttt tgtc
//
LOCUS       DAHUWM010000357         1232 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.367, whole genome shotgun sequence.
ACCESSION   DAHUWM010000357 DAHUWM010000000
VERSION     DAHUWM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.367"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..363
                     /locus_tag="NK788_002868"
     CDS             <1..363
                     /locus_tag="NK788_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCH6771149.1"
                     /translation="ENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIAR
                     ALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIIL
                     ELSGKKLKKVNKMNLEVE"
     gene            363..>1232
                     /locus_tag="NK788_002869"
     CDS             363..>1232
                     /locus_tag="NK788_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6771150.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQ"
BASE COUNT          525 a          131 c          226 g          350 t
ORIGIN      
        1 gaaaatatta gattagtaaa taaatcagca gatgaaagta tcttgtttga actaggttta
       61 gataaaaaac aaataaaaag aaatgttatg aaattatctg gtggacagca acaaagagta
      121 gctattgcta gagcattggt atcagatgct ccaataatac tagctgatga gcctactggt
      181 aatctagaca gtgttactgc tggagaaata attaatatat taaagacatt agctaaagat
      241 agaaataaat gtgtaatagt tgtaacacat agtaaggaag tagcagattc tgcggatatc
      301 attttagaac taagtggtaa aaagttgaaa aaagtaaata aaatgaattt ggaggttgaa
      361 taatgataaa aaatgcattt gcttatgtaa ctagaaaaag tttaaaatca ttaattatca
      421 tattagttat tttatctatg tcaactttaa gtctcattag tttatctatt aaagatgcta
      481 cggacagagc ttcaaaagaa acatttgcta atataacaaa tagtttttct atggagataa
      541 atagacaagt aaatccagga acacctagag gtgggggaaa tgtaaaaggt gaagatatta
      601 aaaagatatc tcaaacagat agtatagact cctatgtaaa aagaataaac agtgttgcag
      661 atttagttga ttatgatatt attgaaactc aagagactct tgcgaatcaa tcacctgaaa
      721 gagcaaagaa ttttaaaaga acagttatgt taactggggt taacgactca acaaaagaaa
      781 caaaatttgt atcagaagca tataaattag tagaaggaaa gcatttagaa aatgaagata
      841 aaaataaaat cttaatgcat aaagatttag ctgaaaaaaa taaccttaaa gttggagata
      901 agataaaaat aaaatctaat ttatttgatg ctgataatga gaaaggtgca gatgaaacag
      961 tagaagtaga aattaaaggt ctattcgatg ggcataatag cggtggagta agtgcagcac
     1021 aagaactata cgaaaataca ttaatcactg atattcatac agctgctaaa gtttatggta
     1081 ataccgaaga tacagctgta taccaagatg caacattctt tgtaaaaggt gataagaatc
     1141 ttgatagtgt aataaaagat cttgggaaat tagatataaa ttggagagaa tataatttga
     1201 ttaaaagttc atcaaattac cctgcattac aa
//
LOCUS       DAHUWM010000358         1230 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.368, whole genome shotgun sequence.
ACCESSION   DAHUWM010000358 DAHUWM010000000
VERSION     DAHUWM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.368"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            93..506
                     /locus_tag="NK788_002870"
     CDS             93..506
                     /locus_tag="NK788_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCH6771151.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            1021..>1230
                     /locus_tag="NK788_002871"
     CDS             1021..>1230
                     /locus_tag="NK788_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771152.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSA"
BASE COUNT          400 a          182 c          212 g          436 t
ORIGIN      
        1 aaagctaacc attatacttg catttttttc aaaatagagt tattacgtaa atagggataa
       61 ataaaaaata cttattgaaa ggatgatcaa aaatggcaaa tgtacctagc attcgcgaca
      121 tgttccctga tttcaatgac gtgtttagtc cagcattcaa tgatttttta ggtgtttcaa
      181 gttatccaaa agtagattta gtcgaaaatg aaaaagaata caaactaact gctgatatgc
      241 ctggctgtga caaagaagac acgacagttg aatattcaga taatactttg acgatctccg
      301 ccaatcatga gtcccataca gaagataaag aagatggcaa ctatgtacgg aaagaaagac
      361 attcggtctc ttacaaacgt tcattctatc ttcctaatgt tgatgaagaa aaaatcactg
      421 gaacctttaa aaacggtgtg ctgaaattgg tattacctaa aacagcttat cagccaaaag
      481 aaacaaaaaa aattgagtta aattagaact tatcgcctct cgatgaatga ggggcgattt
      541 ttatatattt ggataagaac gagtatgttt aaattttcaa aatgagcaaa aaaatatttt
      601 gtttttacga taaagatgtt atgatctctc ttggtaaaaa atatgggagt tgatcatttg
      661 attgtcttaa gtaaactttt gttttggata ccatttatca taatgaccag tgctttcttt
      721 tacttattca aaaggcctaa caaatactta ctcttatctg cttgtccgat gatctatttt
      781 atccataaga ttagaggatt ttattattac gactctggta tctcattgtt ttgctacgaa
      841 atcggcttat tagcttcggc catctttttc atatttgtgt tagaatattt cagaaaaaaa
      901 taaacttcca gtcagttttg gctggaagtt ttttatttgg tgcagatctt ttcagctgtt
      961 attcatacaa gttgatccat ttttctatta ttaagtacat accattgtta ctctttgaat
     1021 gtgggggaga aaaaaataaa aggagctggt attttgttag ttatgagtaa agctttgttt
     1081 ttacttccct gcacgttcat gtttattgtt ttatgttatt tcaaaagaag aaaaaaatgg
     1141 tgctggatcg atgttctact attccttccc tttttcccgc ttatttattt tcttgaagaa
     1201 atatgggatg atatggcatg tgattctgct
//
LOCUS       DAHUWM010000359         1219 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.370, whole genome shotgun sequence.
ACCESSION   DAHUWM010000359 DAHUWM010000000
VERSION     DAHUWM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.370"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(221..1081)
                     /locus_tag="NK788_002872"
     CDS             complement(221..1081)
                     /locus_tag="NK788_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6771153.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          364 a          233 c          178 g          444 t
ORIGIN      
        1 gtgttgctta tttccctcta actatcgttt tctatttgat ggttaaccta cttaaaataa
       61 atactgcaac gacaaacgga gatcaagcat gctttgtaac gtattttacg ataattacga
      121 ctttatttta ttactctatc ttgctgccat acccgtctat ccctaatcat ctttggtgaa
      181 ttttttcaaa gttagcataa caaaaataag tattttttcc ttagttaatc aatagtatca
      241 tttttttcat atgatttaat tcatcaatta attttttgtt tttattaatt ttagcaaaag
      301 ctagtgcatc ttgaatcaat tcgagcactt ctgccttcga cttattttta gcctgtgcgt
      361 tcttcgctaa ttgggcagca gctcgtgcta agtaataagt caaatggtta tccgaacaaa
      421 tatcgattgc gtaatttagt agtgcatcac ttgtttccaa atcaccgtct tgcgcaaaaa
      481 aaacaccaca atgaaagacg atatttaaca cccgccagat atcttctgtt gtacgaatcg
      541 gatacgtata aattttttca aacactcgat tgaaataaac ttctgctttt tccaactctt
      601 tttctctagc atagaccatt ccaatacctg tatatgccaa taaacgaaac aacgtgttgg
      661 ctgtttctgc catcaacaac tgatcaaaat ggaacaacac atccgtaatt ggtgcattcg
      721 taaaaatcat ttcaaatcct tttagatagt agtaacgtgc aatcagctca gactcgtcaa
      781 ttaccttggg atcaatggct gctaaaatct gtgtcaattg ttcatattca ctcgtaatca
      841 gtgaaaactc tgctttttct agctgttcat caagttgtgt attgatcacg ttcatcgaag
      901 aaaaaagttc aactaacgga agattgattc gattacataa tttgattaaa atttttaacg
      961 aagggacttg accattgttc tcaaatttac ttaatgttgc ttgtgtacaa attccttcag
     1021 acacttcttt ttgagataag ttggcagcct ttcttgccgt aataaatttt tcaatgttca
     1081 ttattttctc tccaatcaga cctcacacga aaatcatttc tgtctcgatt ttagcatgtt
     1141 ttctttggga atgctttagg ttctgttgca aagttttaaa taaagaataa agtcccttac
     1201 ggtatctatg atttaagct
//
LOCUS       DAHUWM010000360         1215 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.371, whole genome shotgun sequence.
ACCESSION   DAHUWM010000360 DAHUWM010000000
VERSION     DAHUWM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.371"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            414..947
                     /locus_tag="NK788_002873"
     CDS             414..947
                     /locus_tag="NK788_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCH6771154.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAHUWM010000361         1208 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.372, whole genome shotgun sequence.
ACCESSION   DAHUWM010000361 DAHUWM010000000
VERSION     DAHUWM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.372"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..1032)
                     /locus_tag="NK788_002874"
     CDS             complement(<1..1032)
                     /locus_tag="NK788_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCH6771155.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNK"
BASE COUNT          377 a          240 c          188 g          403 t
ORIGIN      
        1 tttattgaac atatatctta ctttatctat ccgactattt agacgacggg tctggcaaac
       61 aggttcgcca gtggtaacct gatatccttt tagctctgct aaacaaacac taagcccatt
      121 tgtaaaaaaa gttaaatcat tgcgataatc ttgaatacat cgagcaggaa tttctccaat
      181 aataatgacc tcattatttt tcagttgagt atttacgata tttgcacaat atttgggagc
      241 atcgttatat gcccgtgaaa gatattcctg tggtgcataa actttaaaac taagatatgg
      301 ctctaacaat tctgttccag cttttctaaa ggcttgctcc agtacaatag gagtaagcat
      361 ccgaaaatct gctggagtac taacagggct atagtataaa ccgtacttaa aacagatttt
      421 acaatccgtc acattccaac catataatcc ttgttcgcaa ccatagcgta tcccttccat
      481 aactgcattt tgaaatgatt gatttaagta tccaagagaa accgagctct catactgcat
      541 tccacttccc aacggaagcg gtgatacaga taaaccaatg gaagcccaga aaggatttgg
      601 cggcacttcg atgtgaatgg tatattctgc attttttaac ggtctctcca tataaatgac
      661 tgtaggctct tttagttcta tctccacatg atacttttct tgcaacagtg cactaatcac
      721 ttccatttgt actttcccta agaaagaaag tataatttca tgtgtcgtag aatccacgta
      781 atatcgtaga agcggatcac tatctgagat ttccaaaagg gcatcaagca acatttctct
      841 ctgttcaggt ttactcggtt caacagttgt ttgtagtaga gggtgcggat tttcaatctt
      901 ttttctctgt ggcaatagtt ttgtatctcc aagaacacta tttaacttca aaaactcatt
      961 ttgcaaaata acaatttctc cagaataagc tctatcaatc ttacataatt caccatttat
     1021 tgaagtatac atttctgtaa cttttatttt ttctttttct gatactctaa ccgaatctcg
     1081 taaatgtagt actccactat aaaggcgtat atatgcaagt aagtagttca gttttggagt
     1141 acaaaatgac aaaatgtact tcaaaatgga ctactttttt cattttaact tacataccaa
     1201 attacata
//
LOCUS       DAHUWM010000362         1207 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.373, whole genome shotgun sequence.
ACCESSION   DAHUWM010000362 DAHUWM010000000
VERSION     DAHUWM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.373"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            1098..>1207
                     /locus_tag="NK788_002875"
     CDS             1098..>1207
                     /locus_tag="NK788_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771156.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          429 a          202 c          188 g          388 t
ORIGIN      
        1 ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc cgttctacaa
       61 tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata aaatatccca
      121 acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg aatatatttc
      181 ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc aaaatcatgt
      241 taactactat caattactaa taaaaactac cactttaagc gttaagaact gttaagttta
      301 gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg taaaactcaa
      361 aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg gtaaaaaata
      421 ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga aacagctaca
      481 aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc catattcacg
      541 cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt tttaggaaat
      601 tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt caccagatat
      661 tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc accgcgaatt
      721 tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa ttaagggatc
      781 aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt aaacaattat
      841 agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga ctttcttatt
      901 ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt tacgagtact
      961 attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac ccccacttag
     1021 cttggatagg ctacaattaa ctagacagaa aaattaaggt gtgtagacta gaagaaaaca
     1081 taccaggagg aatttttatg tctaagagaa cacgaagaac tttttcacaa gaattcaagc
     1141 aacaaatcgt caatctttac ttagctggaa agccacgtgt agaaatcatt cgagaatatg
     1201 aactaac
//
LOCUS       DAHUWM010000363         1195 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.375, whole genome shotgun sequence.
ACCESSION   DAHUWM010000363 DAHUWM010000000
VERSION     DAHUWM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.375"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1076
                     /locus_tag="NK788_002876"
     CDS             <1..1076
                     /locus_tag="NK788_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCH6771157.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAHUWM010000364         1186 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.376, whole genome shotgun sequence.
ACCESSION   DAHUWM010000364 DAHUWM010000000
VERSION     DAHUWM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.376"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..993)
                     /locus_tag="NK788_002877"
     CDS             complement(<1..993)
                     /locus_tag="NK788_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771158.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESI"
BASE COUNT          475 a          175 c          194 g          342 t
ORIGIN      
        1 gattgactct ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc
       61 ttcaggtgct ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga
      121 gagatgcaat attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt
      181 agaaaaaata taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc
      241 atttaaccgc cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg
      301 aaataaattg gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc
      361 agcagttaat ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc
      421 aatagtatag ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat
      481 accacttaat tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa
      541 aggaattaag aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa
      601 gagtctagcg attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa
      661 tacagaaacc atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga
      721 caaccagtac catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa
      781 aggcaaaaat gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc
      841 acgataggcg gaaaatgtat aaataatcga aaataacagc gaaatataac gattgttttt
      901 tatttgtttc atagaaaaat aagaaacgac cattgttaaa aaagtgataa aaaaatagta
      961 gatattataa cttaaaacaa gatttcccat cattttataa aataaaaaag ctggataaag
     1021 agtaagccat ggataaaaaa tattcatcat tgttccatga tgatcgaagt tattgaagtt
     1081 tactggagaa ctccatacat tatctagtcc tatgattcga gataaatgaa agtaagtatc
     1141 ctgattgaca ctatgataga ttcccttaga catgaataga gggatg
//
LOCUS       DAHUWM010000365         1173 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.377, whole genome shotgun sequence.
ACCESSION   DAHUWM010000365 DAHUWM010000000
VERSION     DAHUWM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.377"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            158..>1173
                     /locus_tag="NK788_002878"
     CDS             158..>1173
                     /locus_tag="NK788_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6771159.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAA"
BASE COUNT          460 a          163 c          200 g          350 t
ORIGIN      
        1 atagagaaaa aagcctgtga tataaatatt gcaggctttt ttattgattt gaaataatca
       61 agaatattag aaacttctac attaatcaag aactttcttc tgtatgaaaa tagagataat
      121 aattgtacac ttaagctgta ttcaggaggt gaagataatg ggcatcgaat ttagcgaacg
      181 gcaaatgaaa atactatact ttttgattga aaatgagcac acaacttaca aagaattatc
      241 agaacaatta tatgtatcta cgaaaacaat tagcaaagat attttaattt tgaaagaaat
      301 aatccaaaaa atgccagatg tacaaatgtt ggttcaacct aaaaaaggcg tttcgcttat
      361 tggaaaaaaa gaaacacttc gaaatttatt atccagtcat aaaatcaata tcgcagactc
      421 tccggaggac cgtctaactt atgtgtttgc taaattattg aatactaatg attctctaaa
      481 gattcaaaat cttgctgacg aattatacgt gagcaggtcg acggtagaaa gcacaatgaa
      541 agatgttaga aaaagactaa acaagcatgg gatgtcctta gtttcagatc gaaatggcgt
      601 gcgcatcaaa gctgatgaaa aaagtaaaag aaggttgatg tcagaagttg tgaactatta
      661 ttggggtggc tttacagcat ctaacagtaa aacagatctc ttagacttga atatcaattt
      721 cattggtgaa gcgaataatt tcttaaataa ggacatattg aaaaaagtag ccgaattgct
      781 taatcgatac atgaataaga cacaattgaa atttacagat tatgaatttc aaactttagc
      841 tatccacctt gctattgcga tagaaaggat tgaaacaagc aatttcttat cacgtagtaa
      901 taattctgaa gaagaaatct caaagaatac acttatttta atcaaaatga ttgaaaaagc
      961 attttcaatt attgtgccaa tagatgaaca agcttatgtt gacattcaca ttacttcgat
     1021 tgaaaaaaat gctatgaatc acgacttaga tcaaacgaag ctttcctatt tagatatggg
     1081 tgttgagcta caagagttag taagaattaa attaaatgaa ttttatcctg atgaagaatt
     1141 gatcaataat ctagtattac atctcaatgc agc
//
LOCUS       DAHUWM010000366         1162 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.378, whole genome shotgun sequence.
ACCESSION   DAHUWM010000366 DAHUWM010000000
VERSION     DAHUWM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.378"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(257..1129)
                     /locus_tag="NK788_002879"
     CDS             complement(257..1129)
                     /locus_tag="NK788_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCH6771160.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          318 a          277 c          225 g          342 t
ORIGIN      
        1 atttgagtgt aacataaatg gcttttttat ttgtctcgct taattataca aacggcgtgt
       61 aaaaaactat tgcacaatac aagtggccta cggctatcga aatttccata actttcgagc
      121 aagaatttat ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc
      181 tgcctaactg gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg
      241 aagagccttt tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga
      301 gcgttccagc gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt
      361 cgacataccc attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt
      421 gtttcatcac accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt
      481 aggcacattg agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct
      541 cttgtccttt tttccctaag cgtttttcga tggcgggtcc agcggccatc ccttctaaac
      601 agttgccatg aaatggacag ctgccagcaa agtcatcttt tggatgaggg acgacgagca
      661 tatgccccat ttctggatga ctaaaccctt cgataaagcg cccttcttgt aaggcaccgc
      721 cgccaacacc cgtaccgatc gtatagtaaa cgacactgct caatcctttg ccactgccga
      781 agacatactc accataggca gccgcattga catctgttgt ccaagaaatc gggatcggaa
      841 attctttttt catggtaccg atgaaatcaa aattttgcca tgccaatttc ggggtcgagg
      901 taatgtaccc ataagtggcg gaatcgcgat ggatatcgat aggcccaaat gagccaatcc
      961 caatccctgc taaatctgct tggtattgtt tgaagaagtc aatgactaaa ggcattgttt
     1021 cctcaggtgt cgttgtcgga aagctcactc tttccttgat cgtcatttct tcatcgccaa
     1081 tggcacacac aaattttgtg ccgcctgctt caattgcacc gtatagcata gacatttctc
     1141 cctttatttt agaatgaaat ca
//
LOCUS       DAHUWM010000367         1154 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.379, whole genome shotgun sequence.
ACCESSION   DAHUWM010000367 DAHUWM010000000
VERSION     DAHUWM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.379"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..1090
                     /locus_tag="NK788_002880"
     CDS             <1..1090
                     /locus_tag="NK788_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6771161.1"
                     /translation="TRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQL
                     TKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDE
                     FKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMN
                     ASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKR
                     YWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFV
                     KRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKL
                     IKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          363 a          247 c          220 g          324 t
ORIGIN      
        1 gacacgcact cagatattga aagtcaaaga aaagttaact attttagaat taaaacgtac
       61 ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact tagttgatga
      121 acatcatcaa ctaacaaagg aactgaagca ggcaattctc atggaactgt acgaaaatca
      181 atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga cacggatctt
      241 gcgtgaagca acgaagcatt accagtcacg tatgaacttc cttccgacgg tgttgtgtat
      301 ggatgagttc aaatcaatga agtctgtttc tggttccatg agttttattt gtgttgatgg
      361 gacaacccat cagctattta cgatccttga agatcgcaga ctttacaaac taactcaata
      421 cttcctgcac tttcctagaa aagcccgttt aaaggtcaag tacctagtga tggacatgaa
      481 tgccagctat tgtcagttgc ttaaaacagt ctttccaaat gcggagatcg taactgaccg
      541 ctttcatatt gtccaacaca tcaatcgcag cttcaatcaa ttacgtgtcc agatcatgaa
      601 tcgttttcga acctcacact ctgaaaatca gaagaagtac cgtcggctca agcgatattg
      661 gaaactctta ctcaaagatt ccacgacatg ggagccactc aaacggcatt atcatcgact
      721 gtttaaacga ccaattagtc aaacagaaat cgttgatgag ctgttaagct acaacgaaga
      781 attgcgaact gcgtatcatt tttgtcaatt attacgctat tatttcgtta aacgaggtac
      841 ggaaggcttt caagaaaccc ttaaaacaat ttcatctacg ttacctcagt cgtttaaaaa
      901 gaaattcacg atcttccatt gctaccaatc gggtatttca aatgccttta aggtgtccca
      961 ctcaaacggc gtcactgagg ggctgaataa caagatcaag ctgattaaac gaattgcgtt
     1021 tggttatcgc aacttttata actttcgggc acggatctat ttacaacaag gtctgatctt
     1081 tgaaaactaa aaaaatatgc ggcgcacaga acgcgccgca tatcggacta agttttattc
     1141 aaatttgtct tcac
//
LOCUS       DAHUWM010000368         1144 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.381, whole genome shotgun sequence.
ACCESSION   DAHUWM010000368 DAHUWM010000000
VERSION     DAHUWM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.381"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="NK788_002881"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="NK788_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCH6771162.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DAHUWM010000369         1135 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.382, whole genome shotgun sequence.
ACCESSION   DAHUWM010000369 DAHUWM010000000
VERSION     DAHUWM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.382"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(241..1089)
                     /locus_tag="NK788_002882"
     CDS             complement(241..1089)
                     /locus_tag="NK788_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCH6771163.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          355 a          194 c          196 g          390 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctc
//
LOCUS       DAHUWM010000370         1135 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.383, whole genome shotgun sequence.
ACCESSION   DAHUWM010000370 DAHUWM010000000
VERSION     DAHUWM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.383"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(406..780)
                     /locus_tag="NK788_002883"
     CDS             complement(406..780)
                     /locus_tag="NK788_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCH6771164.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..>1135)
                     /locus_tag="NK788_002884"
     CDS             complement(803..>1135)
                     /locus_tag="NK788_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HCH6771165.1"
                     /translation="LKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQMFDKGLQR
                     PLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDY
                     SINYECGF"
BASE COUNT          386 a          213 c          137 g          399 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaa
//
LOCUS       DAHUWM010000371         1133 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.384, whole genome shotgun sequence.
ACCESSION   DAHUWM010000371 DAHUWM010000000
VERSION     DAHUWM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.384"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            79..702
                     /gene="lexA"
                     /locus_tag="NK788_002885"
     CDS             79..702
                     /gene="lexA"
                     /locus_tag="NK788_002885"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCH6771166.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            882..1100
                     /locus_tag="NK788_002886"
     CDS             882..1100
                     /locus_tag="NK788_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771167.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          350 a          215 c          220 g          348 t
ORIGIN      
        1 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
       61 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
      121 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
      181 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
      241 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
      301 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
      361 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
      421 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
      481 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
      541 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
      601 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
      661 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
      721 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
      781 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
      841 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
      901 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
      961 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     1021 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     1081 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAHUWM010000372         1127 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.385, whole genome shotgun sequence.
ACCESSION   DAHUWM010000372 DAHUWM010000000
VERSION     DAHUWM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.385"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(84..1010)
                     /gene="cysK"
                     /locus_tag="NK788_002887"
     CDS             complement(84..1010)
                     /gene="cysK"
                     /locus_tag="NK788_002887"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCH6771168.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          298 a          267 c          177 g          385 t
ORIGIN      
        1 tttttctcat cataaatttt tgtgtgttct tttcatcttt aaaagttttt tacataaacc
       61 tatctggaga aaaaccaagt gccttatatc tcttggtaaa gggctgtaga taaataacgt
      121 tcaccattgt cgggaaccac agctaatacc ttcttgcctt ttcctaattc ttttgcgact
      181 tttagcgcgg cagcaattgc agcgccagat gagatgccta ctaaaatccc ttcttttcgc
      241 ccaacttctc tagctgtttc catcgcttcg tctccactaa tagaaaggac tttatcatac
      301 acagaagtat ccagtgtttt aggaacaaaa cctgtaccga ttccttggat tttgtgaggt
      361 cccgcttctt tcccttctaa aatagcagat tctgctggtt ctaccccgat taattcgatt
      421 ttcggataca cacgttttaa ttctcggcca gcgccagtga tcgttcctcc tgtaccgata
      481 cctgcaacaa acgcgtccaa gccatttact ccaaatgctt gatgtatctc aggaccagtc
      541 gtcttctcat gcacaagagg atttgcttca ttttcaaatt gtaatggtaa aaagtagccg
      601 ttttcttttg ccaaacgttc tgcttctctg attgatccag aaataccatc cgcaccagga
      661 gtcaaaatca actctgcgcc gtaccctttc atcaataaac gtctttcgat actcatcgtc
      721 tcaggcatca cgatgattac tttatatcct tttgcaactc ctaccatcga caatccgatt
      781 cctgtatttc cagaagtagg ttcaatgata gtatcacctg gtttcagtag accatcgtgt
      841 tctgcttttt ctatcatact taaagcaata cgatctttta cacttcctcc tggattaaaa
      901 aactcaagct ttacaaaaac atctgctgaa tcttctggta cgattttatt taatttgacg
      961 attggcgttt tgccaatcaa ttcggtgact gagttataga tagctgtcat cttcaattcc
     1021 tccttgtctt tgctcttctt acttatcata atacaatctg ttcatttatg gcgagtgatt
     1081 cgtctttgac tagcaaaaaa aagacaaaga tataccatat atggtaa
//
LOCUS       DAHUWM010000373         1116 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.386, whole genome shotgun sequence.
ACCESSION   DAHUWM010000373 DAHUWM010000000
VERSION     DAHUWM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.386"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..898)
                     /gene="vanY"
                     /locus_tag="NK788_002888"
     CDS             complement(<1..898)
                     /gene="vanY"
                     /locus_tag="NK788_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCH6771169.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          160 g          400 t
ORIGIN      
        1 tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg tttcctgata
       61 tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca ggataataaa
      121 agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct tttaggtaat
      181 ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt ggtaaaccaa
      241 catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg tcctctggat
      301 aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct tcaggggctc
      361 gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta tgctcactat
      421 aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt tgctcatcaa
      481 agtctcgata gccactatta ataataaaat gactaacgcc accctttaca gcatcattga
      541 ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta tcaagcaacc
      601 cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac ttcacacttt
      661 cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa acctgttctt
      721 ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa tgttctttgg
      781 gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct tcattaacgt
      841 agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac ttcttcattt
      901 tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat cttcccctta
      961 aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag tcaattattt
     1021 cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt gatacccatg
     1081 ctgaatttaa cgaggctgaa ctgcctgttg acaatg
//
LOCUS       DAHUWM010000374         1103 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.387, whole genome shotgun sequence.
ACCESSION   DAHUWM010000374 DAHUWM010000000
VERSION     DAHUWM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.387"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            69..1016
                     /locus_tag="NK788_002889"
     CDS             69..1016
                     /locus_tag="NK788_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCH6771170.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          354 a          170 c          235 g          344 t
ORIGIN      
        1 tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag
       61 gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag
      121 ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag
      181 tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat
      241 ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag
      301 ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa
      361 gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt
      421 ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg
      481 tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag
      541 attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc
      601 caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata
      661 agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta
      721 gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag
      781 ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga
      841 ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt
      901 cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac
      961 cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca
     1021 tactaagata agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt
     1081 gtaaaatcaa atctgggata gaa
//
LOCUS       DAHUWM010000375         1091 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.388, whole genome shotgun sequence.
ACCESSION   DAHUWM010000375 DAHUWM010000000
VERSION     DAHUWM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.388"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            45..587
                     /locus_tag="NK788_002890"
     CDS             45..587
                     /locus_tag="NK788_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771171.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            743..>1091
                     /locus_tag="NK788_002891"
     CDS             743..>1091
                     /locus_tag="NK788_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCH6771172.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          464 a          114 c          214 g          299 t
ORIGIN      
        1 ataaaaaatt atatttcatc aacgtttcat aaaaggtgtg tagaatgtta ttgaaagtag
       61 ctataaaatg ctattgtgta aaaaataaag gaggaataat ttatgaaaaa atagtaccaa
      121 gaggagcatg cctattactt gcattatgtt tgagcttaag cgcagttggg tgtcaaaaaa
      181 gtaatgaaaa caataaaata aaagaaggac aatccataag ttctgaagag gaaggcatgt
      241 caattaatgc aaatggcaaa aatgaaactt ttaagccatc agattataca ttggaggcaa
      301 agaaagaata tgtttatgaa tatttagggc ttaaatttaa actctcagac aaatttagaa
      361 attatatagc tgataaaaag atagcgatgt tagatgatca aagtcctata gataaagaac
      421 taaagtatgc tattttgaca tttgagaaaa tgacagaaga acaaaagaat gcagttatag
      481 aaaagatggg cgatggatat aaaaattggc aaaatgagct tgaaagaatt gggactattg
      541 gtatatttga aaaaaataca tcagaagaaa aaaatctaaa attataaaat gtgatactca
      601 tactaaaata ggagtttcaa gtgatggaaa atacgattgc tatttaagca caaatagcgg
      661 agctgaaagt aatcttctgg atgaattaaa aagaactgag attcaaatta tagataaaaa
      721 agaacgtcct aaaaatggtt ttgtgctatc agaaaaaact gatttagaaa atacagaagc
      781 atttaacaaa gaatcggtta aagatttacg taaactacca acaaaggaca taaatggcaa
      841 ggattttaca agtaaagatt ttgagaagta tgatcttact atggtaaatg tatttgcaac
      901 ttggtgtaca gcttgtgtaa aggaaattcc tgatttggtt gaggttcaga atgaaatgaa
      961 aagcaagggt gtaaatatcg ttggagttgt tacagatcct gttgatgata acggtgaaaa
     1021 caaggaagca attgaaaagt caaagttaat acatgaaaag acaaaggctt catatccttt
     1081 cttaatgcca g
//
LOCUS       DAHUWM010000376         1079 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.389, whole genome shotgun sequence.
ACCESSION   DAHUWM010000376 DAHUWM010000000
VERSION     DAHUWM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.389"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            4..963
                     /locus_tag="NK788_002892"
     CDS             4..963
                     /locus_tag="NK788_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCH6771173.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          376 a          205 c          211 g          287 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaa
//
LOCUS       DAHUWM010000377         1069 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.390, whole genome shotgun sequence.
ACCESSION   DAHUWM010000377 DAHUWM010000000
VERSION     DAHUWM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.390"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            82..>1069
                     /locus_tag="NK788_002893"
     CDS             82..>1069
                     /locus_tag="NK788_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCH6771174.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTS"
BASE COUNT          360 a          196 c          206 g          307 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccc
//
LOCUS       DAHUWM010000378         1066 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.391, whole genome shotgun sequence.
ACCESSION   DAHUWM010000378 DAHUWM010000000
VERSION     DAHUWM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.391"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>1066
                     /locus_tag="NK788_002894"
     CDS             <1..>1066
                     /locus_tag="NK788_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCH6771175.1"
                     /translation="ETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDI
                     PGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLF
                     DVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSM
                     DMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRS
                     SVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSE
                     SIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIE
                     EQITLDKLKMLDYLFHPSFS"
BASE COUNT          410 a          128 c          184 g          344 t
ORIGIN      
        1 tagagacaga agttgacaaa attgatattc aaaataaaaa gattagttgt gtaaaaaaag
       61 atggtacaag attctctcaa gaatatgaca aagttatatt agctacaggt tcaaagcaaa
      121 ttatgctaga tataccgggg aataatttag ctaatatttt ttcgataaag aattttccaa
      181 atgcaaaaga gatttttaaa gctttggatg atagtactat taaaaaagta ggaattattg
      241 gagcaggata tattggagtt gaaattgctg aagccataag aaaaagaggg aaagaagtat
      301 atcttttcga tgttgcagat agagtgttgt cgacttatta cgaccgtagt ttttcagata
      361 aagttgaaga aatactatca aaaaatggaa tacatctttg tctatcagaa agtcctaaaa
      421 aatatttagg aagaaaaaag attgaaaaaa tagtcactac aagtggtacc caatactcta
      481 tggatatggt cgtacaagct ataggatttc tatctaatag tccaattggg gaaaaggaac
      541 tcttgagaga tgttagtgga gcatatctaa ctaatgaata ccagcaaaca aatataaaag
      601 atatatatgc tattggggat tgtgctacaa ctttttttac gtccattaat agaagtagtg
      661 ttgatttttc tatttctaat gctctaagaa cagcttatat tgcaactcaa catattaaca
      721 atcaagattt tacaccttct ggttcccaat ttacaaatgg cttttctata tttaattaca
      781 atttttacgc agcaggtttg aatttaaaaa cagcacgatt attaagtgag tctatagatt
      841 atatagaaat agaagatttc attagacctg cttttttgag aaaaaatagt cgtgtaaaat
      901 taagaattag ctttgaaaaa aatacaaaga gaatagtagg tgtacaactt tgtagtcaag
      961 aagatttatc tggactatta tcaatgtttt ctcttgctat agaagaacaa attacattag
     1021 acaaattgaa aatgttagat tatttatttc atcctagttt tagcca
//
LOCUS       DAHUWM010000379         1065 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.392, whole genome shotgun sequence.
ACCESSION   DAHUWM010000379 DAHUWM010000000
VERSION     DAHUWM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.392"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(61..279)
                     /locus_tag="NK788_002895"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="NK788_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="NK788_002896"
     CDS             complement(545..739)
                     /locus_tag="NK788_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771176.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="NK788_002897"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="NK788_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          371 a          184 c          154 g          356 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caatt
//
LOCUS       DAHUWM010000380         1061 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.393, whole genome shotgun sequence.
ACCESSION   DAHUWM010000380 DAHUWM010000000
VERSION     DAHUWM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.393"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..410
                     /locus_tag="NK788_002898"
     CDS             <1..410
                     /locus_tag="NK788_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCH6771177.1"
                     /translation="GVLFYTQIATSEKGFNFDWQTTLFVSVIYFALLLGIVIAYFLLK
                     LLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDDAPGLMLVGIA
                     VVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            419..646
                     /locus_tag="NK788_002899"
     CDS             419..646
                     /locus_tag="NK788_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6771178.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          331 a          183 c          181 g          366 t
ORIGIN      
        1 tcggcgtact tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc
       61 agaccacatt atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt
      121 atttcttatt gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct
      181 taaacatact aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct
      241 tacttcctaa atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag
      301 gaatcgctgt cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac
      361 tgcttgaaca tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat
      421 ggcaattatc gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt
      481 gagtgagaaa gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa
      541 agccatccgt ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga
      601 tttgttgacc tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca
      661 aacagtaaat aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg
      721 tctattattc agatttttta tttcgattga ataccgtttt atctataaat aagatatgta
      781 ctgactcata caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact
      841 ggtttcctgt tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca
      901 gactgatagc agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca
      961 taccttgaag tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct
     1021 tttttagtaa gctcccatat atactgtaaa gccttctata t
//
LOCUS       DAHUWM010000381         1058 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.394, whole genome shotgun sequence.
ACCESSION   DAHUWM010000381 DAHUWM010000000
VERSION     DAHUWM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.394"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..384
                     /gene="spx"
                     /locus_tag="NK788_002900"
     CDS             <1..384
                     /gene="spx"
                     /locus_tag="NK788_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCH6771179.1"
                     /translation="CTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEGGV
                     QDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDEIR
                     SFVPREIRCVARNLIYLQNNNILNS"
     gene            398..754
                     /locus_tag="NK788_002901"
     CDS             398..754
                     /locus_tag="NK788_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCH6771180.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          367 a          158 c          203 g          330 t
ORIGIN      
        1 tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga atcaacttga atttgaagag
       61 aagaatattt ttgccgaacc gttaacagaa agtgagatca aaaagatctt ggcagcgact
      121 gaaggaggag ttcaagatat tatttccact cggtcgaaaa tttacgaaaa gctgggcata
      181 gattttaacg aattaacttt aaagcaaatg atcgtcttat tcaaagaata tccttcatta
      241 ttgagacgac ctattttgat tgatgaaaaa agaatactaa tcggatttaa tgaagatgag
      301 atccggtcat ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat ctatttacaa
      361 aacaataaca tcttaaattc atagggaggg agccttcgtg gaagtgaaag tctatggatc
      421 agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc aattgcctta
      481 tcaatttgtt gacttagaag aacgcgatct aagtacagaa gaagcagaag aattatgcag
      541 ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg tctgaggctt ttcacaacat
      601 tgcgattgac ctttcctgtg taggaaaaaa acaactgatt tttttatgca cacaacatac
      661 caatctcctc agacgtcctt tgattattat cgatgaagtt ctatttattg gatacaatga
      721 gcagttgatg gaacagctta tcttaaataa atagacttta actgttttaa tcaagtcact
      781 gagcatttca tacagttgta acagtcgaac acttagcatc aagcgtgtta tgtgttcttt
      841 ttttgacttt atttttctac tatagagaca tgctcaattt atgtggtcaa aattcaaatc
      901 taggtgggga tatagagtta aaaatatatt gacatgtgta aatttacatg atatattcat
      961 ttctgttgtt ttaagtagca aagcaataaa atagtatttt aaaaaacata taaaatagtt
     1021 gttgacatgt tagaaatgac atgataagat aactgagt
//
LOCUS       DAHUWM010000382         1035 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.395, whole genome shotgun sequence.
ACCESSION   DAHUWM010000382 DAHUWM010000000
VERSION     DAHUWM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.395"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..825
                     /locus_tag="NK788_002902"
     CDS             <1..825
                     /locus_tag="NK788_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCH6771181.1"
                     /translation="VHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVL
                     RKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKEL
                     DISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVE
                     LDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKD
                     TKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            858..>1035
                     /locus_tag="NK788_002903"
     CDS             858..>1035
                     /locus_tag="NK788_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCH6771182.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKN"
BASE COUNT          402 a          116 c          193 g          324 t
ORIGIN      
        1 gttcacaatc aggtttattt tggcggggga attaatttat ggttagccaa aaagaatgga
       61 atcagacaga gaattgctca tagccatgca actgaagatg gaaaatctca aaatattgta
      121 atgaatgtat tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac agactacctt
      181 gccgttagtc aagaagctgg tgaaagtctg tttcaaaacc atccctttga gatagttcat
      241 aatggtattg atttagagct ttatagtaaa aattcagaag ctaaggttaa taagagaaaa
      301 gaattggata tctcaatgag tacttttgtt gtgggaaaca ttggacgatt agagaaacaa
      361 aaaaatcaat cttatttgtt agagattttt ttagaaatac taaaaatgaa ttctgattct
      421 tacttgattt tagtaggtga agggtcactt agaaataaac tgaaagccaa agcagtagaa
      481 ttggatatag agaaaagagt aaaattttta ggagaaagaa acgatattcc cgagttatta
      541 tctgtgatgg atgtttttgt aatgccttct ctttatgaag gtctgccaat ggttggatta
      601 gaagcacaag cagcaaaatt aaaattagtc ttagcagata ctatttccaa agatacaaag
      661 ttaacaaata atgtggattt tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa
      721 gtaataaaag agccgtatgc taataaaata acacaagaac taaagagtta tgatgtttcg
      781 tacactactg aacaaatgga gagtatatat agtagagatc attaaaaatg ttcaagatat
      841 tttaggagtg attatttttg aaaatatgtt atttgatatt ggcatacaat gatattggca
      901 acttaaagag attggtcaat cgattgaacg aaaatgcagt ttttattatt catatagata
      961 aaaaaactga tatagaacca tttattgaag catttaaatc atatgataat gttgaatttt
     1021 tatgtaataa aaacc
//
LOCUS       DAHUWM010000383          995 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.396, whole genome shotgun sequence.
ACCESSION   DAHUWM010000383 DAHUWM010000000
VERSION     DAHUWM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.396"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(26..913)
                     /locus_tag="NK788_002904"
     CDS             complement(26..913)
                     /locus_tag="NK788_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCH6771183.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAHUWM010000384          994 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.397, whole genome shotgun sequence.
ACCESSION   DAHUWM010000384 DAHUWM010000000
VERSION     DAHUWM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.397"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..>994)
                     /locus_tag="NK788_002905"
     rRNA            complement(<1..>994)
                     /locus_tag="NK788_002905"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAHUWM010000385          986 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.398, whole genome shotgun sequence.
ACCESSION   DAHUWM010000385 DAHUWM010000000
VERSION     DAHUWM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.398"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..704
                     /locus_tag="NK788_002906"
     CDS             <1..704
                     /locus_tag="NK788_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCH6771184.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAHUWM010000386          980 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.399, whole genome shotgun sequence.
ACCESSION   DAHUWM010000386 DAHUWM010000000
VERSION     DAHUWM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.399"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            50..583
                     /locus_tag="NK788_002907"
     CDS             50..583
                     /locus_tag="NK788_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCH6771185.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            599..769
                     /locus_tag="NK788_002908"
     CDS             599..769
                     /locus_tag="NK788_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771186.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
BASE COUNT          406 a          169 c          167 g          238 t
ORIGIN      
        1 tatctgattt agataattta ttccagtagc aacctaggag gcaaccagta tgcaaccaca
       61 agaaaccttt acaataaagg aattgtcaga actttttaag atgagtaggc aagcaatatc
      121 gaaacatatc caaaaattag attcttccat gatagctaaa aatgagcgtg gttataaggt
      181 tgtattgcgt tctggagtat tgcaacttgc tcgcaactta ggcaaccaaa tgttgctaga
      241 taggttgcac gagtacgaac aagacaacga taaaaatgtt aatataccaa gcaaaaacgt
      301 ggttacaaac aagttgctag atcagttgcg tgaagaaaat gaatttttac gagaacaact
      361 tgccctcaga gagcaaaata attctcatat acaaaaacta ctcgaccagc aacaacaact
      421 caccctacaa gccaacaaac aaatcgaaaa actgcaagaa aaactacagt tgacttatac
      481 agaagaagct ccagagaatg actcaacgac tctttctgaa gcaacagaaa caatagacaa
      541 acaaattgat actgaaaaaa aatggtggca actttggaaa taactggagg gaacattcat
      601 gacaagaaaa aatcttctat tcggttctat tttactagga attctaggat tcagtccgat
      661 gttattgtca gtgatcaata aagggaataa acatggtgaa ttgaaattga atccaaatta
      721 ttacaacagt cctaacgaag aattgtttgt aaaagaaaca gataaataat aaaaaagact
      781 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      841 tatgaagcgc taccaaaaat ttatcatatt ttattaaaaa taacaaccca gtaaaaaatg
      901 actggtaaaa attcacaaag aaacggaaag aagcaaccgc agaaaatcaa gcttaaaaag
      961 agcttttgac ctagaccaat
//
LOCUS       DAHUWM010000387          971 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.400, whole genome shotgun sequence.
ACCESSION   DAHUWM010000387 DAHUWM010000000
VERSION     DAHUWM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.400"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..746
                     /locus_tag="NK788_002909"
     CDS             <1..746
                     /locus_tag="NK788_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCH6771187.1"
                     /translation="ENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRN
                     LENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQ
                     ENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEI
                     ANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSS
                     HFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            763..>971
                     /locus_tag="NK788_002910"
     CDS             763..>971
                     /locus_tag="NK788_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771188.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISH"
BASE COUNT          364 a          110 c          172 g          325 t
ORIGIN      
        1 cagaaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta tcacaaaatt
       61 acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat gttaaaaatg
      121 gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata attccaagtg
      181 ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt gaaaagttag
      241 cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt agagatgctt
      301 tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg agggattctg
      361 ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt tctcccttaa
      421 tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact gtaaaggaaa
      481 tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag aatgaaatgc
      541 atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa ataatgttga
      601 aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat tcttcacact
      661 tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag
      721 tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact atatcattta
      781 cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg ggaaatttct
      841 gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag tgtgttaaat
      901 gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc tagagaatct
      961 atcagtcata t
//
LOCUS       DAHUWM010000388          966 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.401, whole genome shotgun sequence.
ACCESSION   DAHUWM010000388 DAHUWM010000000
VERSION     DAHUWM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.401"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            82..>966
                     /locus_tag="NK788_002911"
     CDS             82..>966
                     /locus_tag="NK788_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCH6771189.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANI"
BASE COUNT          327 a          180 c          191 g          268 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatc
//
LOCUS       DAHUWM010000389          964 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.402, whole genome shotgun sequence.
ACCESSION   DAHUWM010000389 DAHUWM010000000
VERSION     DAHUWM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.402"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            331..964
                     /locus_tag="NK788_002912"
                     /pseudo
     CDS             331..964
                     /locus_tag="NK788_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          326 a          181 c          177 g          280 t
ORIGIN      
        1 atttttacca gaaagaaatg aacaccttca attatctaaa tctcgaaaat ggttagatgt
       61 aatcatcagg aaaaacaatc ttaaacgaat tactattcat ggtcttagac acacacatgc
      121 tagcttgctt ttagcaagct agtgcaaata ttaaagatgt gcaagagcgt ttaggccact
      181 cttctattca aatcattatg gatctatata ttcatattac agacaaaaga aaagaagaaa
      241 ccgtagcgca attcgcaaaa tataggctct ataatttatg ggactgatga atcaaaattg
      301 attcttcagt cccattgtta ttttaggtat atgattcgta cattgaaaga gttgaaaaac
      361 tatcttccga agccgtcaaa aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt
      421 tcgcacgctt caattgtgga taaaatgagc tttatttgtg cggatggcga gactggaaaa
      481 ctcattgata ttttgcctac acgaaaattg tctcgattaa cacactattt tagtcgctgt
      541 gcaaatcctg aagcggtgga atatttagtg acagatatga acgccgccta cttccaactg
      601 acaaaacgtg tcttaccaaa tgcgaaagtc gtcattgatt gttttcatat catcaaacat
      661 ttgaatcagg cgttcaatga ttttcgtgta caagaaatga aaagattaca ccagttaggg
      721 caacgacggg aagccgagaa agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa
      781 aaaatcaatc actatgatta caaaacatgg cgcagttttc gtgcacctaa atacccgcta
      841 ctcactgaag caatgatgat tgatcgattg ctggcatttt caacgccttt aaaggacgcc
      901 tatacttttt tccatgagct gaccgaagcc tttagagaca aggaatcaga cttatttttt
      961 acct
//
LOCUS       DAHUWM010000390          962 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.403, whole genome shotgun sequence.
ACCESSION   DAHUWM010000390 DAHUWM010000000
VERSION     DAHUWM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.403"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(37..951)
                     /locus_tag="NK788_002913"
     CDS             complement(37..951)
                     /locus_tag="NK788_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771190.1"
                     /translation="MLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLF
                     FSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESF
                     ENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEY
                     INDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKN
                     LSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILL
                     TIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          308 a          163 c          146 g          345 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 tt
//
LOCUS       DAHUWM010000391          959 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.404, whole genome shotgun sequence.
ACCESSION   DAHUWM010000391 DAHUWM010000000
VERSION     DAHUWM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.404"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(107..511)
                     /locus_tag="NK788_002914"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="NK788_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="NK788_002915"
     CDS             complement(570..>959)
                     /locus_tag="NK788_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771191.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAHUWM010000392          931 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.405, whole genome shotgun sequence.
ACCESSION   DAHUWM010000392 DAHUWM010000000
VERSION     DAHUWM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.405"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>931
                     /locus_tag="NK788_002916"
     CDS             <1..>931
                     /locus_tag="NK788_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCH6771192.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAHUWM010000393          921 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.406, whole genome shotgun sequence.
ACCESSION   DAHUWM010000393 DAHUWM010000000
VERSION     DAHUWM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.406"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            21..244
                     /locus_tag="NK788_002917"
                     /pseudo
     CDS             21..244
                     /locus_tag="NK788_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(449..769)
                     /locus_tag="NK788_002918"
     CDS             complement(449..769)
                     /locus_tag="NK788_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCH6771193.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT          317 a          139 c          180 g          285 t
ORIGIN      
        1 aagaaaggag agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga
       61 aaaagaagag atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga
      121 gcaaattgca caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga
      181 cacgcgtaga actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag
      241 ctagaaagaa aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca
      301 caaaaatttg aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc
      361 tttcgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatt attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt
      481 ttggaatcga gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt
      541 aaacggcgtc tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct
      601 ggattttacg tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga
      661 caatagtggt atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct
      721 ggtttaatac aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct
      781 cttttatgta tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac
      841 agtattttga cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca
      901 cgctataaaa ctggatttat t
//
LOCUS       DAHUWM010000394          921 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.407, whole genome shotgun sequence.
ACCESSION   DAHUWM010000394 DAHUWM010000000
VERSION     DAHUWM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.407"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(50..122)
                     /locus_tag="NK788_002919"
     tRNA            complement(50..122)
                     /locus_tag="NK788_002919"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:His,seq:gtg)
     gene            complement(125..198)
                     /locus_tag="NK788_002920"
     tRNA            complement(125..198)
                     /locus_tag="NK788_002920"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Trp,seq:cca)
     gene            complement(205..285)
                     /locus_tag="NK788_002921"
     tRNA            complement(205..285)
                     /locus_tag="NK788_002921"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Tyr,seq:gta)
     gene            complement(287..362)
                     /locus_tag="NK788_002922"
     tRNA            complement(287..362)
                     /locus_tag="NK788_002922"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Phe,seq:gaa)
     gene            complement(398..470)
                     /locus_tag="NK788_002923"
     tRNA            complement(398..470)
                     /locus_tag="NK788_002923"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Asp,seq:gtc)
     gene            complement(480..552)
                     /locus_tag="NK788_002924"
     tRNA            complement(480..552)
                     /locus_tag="NK788_002924"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Val,seq:tac)
     gene            complement(568..639)
                     /locus_tag="NK788_002925"
     tRNA            complement(568..639)
                     /locus_tag="NK788_002925"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Glu,seq:ttc)
     gene            complement(654..743)
                     /locus_tag="NK788_002926"
     tRNA            complement(654..743)
                     /locus_tag="NK788_002926"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Ser,seq:gga)
     gene            complement(783..856)
                     /locus_tag="NK788_002927"
     tRNA            complement(783..856)
                     /locus_tag="NK788_002927"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(820..822),aa:Asn,seq:gtt)
BASE COUNT          255 a          259 c          209 g          198 t
ORIGIN      
        1 agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaagg gcgaatgatg
       61 ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc
      121 gccatggcag gggcagcagg aattgaaccc acactaacgg ttttggagac cgctgttcta
      181 cctttaaact atgcccctat aaaatggagg agagtggatt cgaaccactg aaccctaagg
      241 aacggattta cagtccgtcg cgtttagcca cttcgctact cctccatggt ggcgcgggac
      301 agaatcgaac tgccgacaca cggagcttca atccgttgct ctaccaactg agctaccgcg
      361 ccatatttta aaattattaa ttgaataaca agaacgacgg ttccgacggg aatcgaaccc
      421 gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc agttcattat
      481 ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg
      541 agctaaacct ccaacattca attaaaatga cccgtacggg attcgaaccc gtgttaccgc
      601 cgtgaaaggg cggtgtctta accgcttgac caacgggcca ggtaaatttg ctacggagag
      661 taagggattc gaacccttga gacagtttgg caccatctac atgatttcca atcatgctcc
      721 ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc tcaaaatgag
      781 aactccggca gtaggactcg aacctacgac atcatgatta acagtcatgc gctactacca
      841 actgagctat gccggaataa catcagcgtg gcgacgtcct actctcacaa ggggcaaccc
      901 ctcactacaa tcggcgctaa g
//
LOCUS       DAHUWM010000395          903 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.408, whole genome shotgun sequence.
ACCESSION   DAHUWM010000395 DAHUWM010000000
VERSION     DAHUWM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.408"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            6..>903
                     /gene="bsh"
                     /locus_tag="NK788_002928"
     CDS             6..>903
                     /gene="bsh"
                     /locus_tag="NK788_002928"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCH6771194.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDM"
BASE COUNT          308 a          122 c          165 g          308 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tga
//
LOCUS       DAHUWM010000396          902 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.409, whole genome shotgun sequence.
ACCESSION   DAHUWM010000396 DAHUWM010000000
VERSION     DAHUWM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.409"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            66..881
                     /gene="thiD"
                     /locus_tag="NK788_002929"
     CDS             66..881
                     /gene="thiD"
                     /locus_tag="NK788_002929"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCH6771195.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          325 a          146 c          205 g          226 t
ORIGIN      
        1 acatatgata gaaacagtaa cagtattatg aaaaatcttt ttataaaaat ggaggaatcg
       61 atttgatgga agatcagtat gtacaagcaa tgacgatcgc aggatcagat agcgatggaa
      121 gcgcagggat gcaagcagat atgcatacat tctttacaag aaatgtttac ggtgtttctg
      181 tcatgaccgc atgtgttgca ggtaattctt acggaatcgg agcaagtgtg acattgccaa
      241 cagattttat cgacaaagaa tttgaactga ttgcgaagga ctttcaaata cgtgcagcta
      301 aaacaggtat gctagcagat tcaaagttga ttgagacagt tgtaaaaaat taccaaaaat
      361 atgattttgg accattagtc gtggatcccg taatcgtaac gaaacatgga aatcttttac
      421 tagaagaatc tgctttgcaa agcttgaaag aaaagcttgt ccctttggcc gaagtactga
      481 cacctaattt ttatgaagca gaaaagctga ctggacggac attagcagct gaagaagatt
      541 ttgtgcaagc agcaaaagaa ctgcaagata tgggtgcaaa aaacgttatg atcaaaggac
      601 atcatgctaa aaatgcgcag caagaaacag tcaatgacta cgtattgttc gaatcaggaa
      661 aatcattttg gttaaatgca ccattttatc ctaccgaccg gatcaacgga actggcgatt
      721 cgctatctgc atgtattgca gctgagttag cgaaaggaaa aacagttgaa caagcgatcc
      781 gattggcaaa agactatgta aaccgtgcaa ttcgtgaaga aataaatgta gggcacaaat
      841 atggccccat caatcattgg gcagctggtg aattagagta aaagaaagaa gttgtgaaaa
      901 ag
//
LOCUS       DAHUWM010000397          879 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.410, whole genome shotgun sequence.
ACCESSION   DAHUWM010000397 DAHUWM010000000
VERSION     DAHUWM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.410"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            86..355
                     /locus_tag="NK788_002930"
     CDS             86..355
                     /locus_tag="NK788_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCH6771196.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="NK788_002931"
     CDS             348..605
                     /locus_tag="NK788_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCH6771197.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          335 a           97 c          168 g          279 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgcttata
//
LOCUS       DAHUWM010000398          878 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.411, whole genome shotgun sequence.
ACCESSION   DAHUWM010000398 DAHUWM010000000
VERSION     DAHUWM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.411"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(624..869)
                     /locus_tag="NK788_002932"
     CDS             complement(624..869)
                     /locus_tag="NK788_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HCH6771198.1"
                     /translation="MIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          292 a          168 c          125 g          293 t
ORIGIN      
        1 atctatcaat aattatcaaa ctgagtcata tagagacaaa aatttcaaaa atgacctgtt
       61 aaatggtcat cgaatatcat ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca
      121 agcccgctga gagtaaaaaa tatacagtcc cataggtaaa gtgccacagg actgtatatt
      181 tttttatttg agattacctg aaatgattcc atttaatttt tatctcattt tttcctacta
      241 gttagtatca aatactctcc tattttctca ataaactatt ttgggttaca tggtatctga
      301 aacagctaaa accttgtcat tatagcaaaa aatcacttta tctccgtttc atttcagata
      361 catttcactt aagcggattc caaaaaaaca aaagaaaaag gaacccctag ttagcctatg
      421 ctttttattg gttcctctca aaacacggtc atataacaac ggggtggggt aaatgaatga
      481 aaaaggtgga ggaaacatta ttcaatagct gtcttaaata cttcgttata aaatgtgaac
      541 aaataaaaga catctatccc tattttctac acttccactt gactaccatc tttgaatcgt
      601 acaattttct taactcttct cgtttacctc aattcattct caagtggaat caactcatca
      661 tctaacattt tacgccatgc tcgcttcatt tcttgtgggt atttaaatgg cttcgcttct
      721 ggatgtttgg ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga
      781 attggatctt cacctggtag atattgcaga ttagaagcat gtaattcttt ctcagaaaca
      841 aaccactcat tggaaaagac atgaatcaaa tcatcact
//
LOCUS       DAHUWM010000399          873 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.412, whole genome shotgun sequence.
ACCESSION   DAHUWM010000399 DAHUWM010000000
VERSION     DAHUWM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.412"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>873
                     /locus_tag="NK788_002933"
     CDS             <1..>873
                     /locus_tag="NK788_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCH6771199.1"
                     /translation="FLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGF
                     FIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTA
                     DRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNT
                     FVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPIL
                     SAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKD
                     IFIKHYHIVVESWG"
BASE COUNT          274 a          111 c          171 g          317 t
ORIGIN      
        1 atttctaata caatttgcta aagggttaga aaaagttaaa gctatgggca tagcaggtat
       61 tatcagcaca attgtaacaa taattgccaa tattttcttc ttgcttattc tgaaatgggg
      121 attaattggt tttttcattg caaatatttt atcacagatt atttcagcta cttatctgtc
      181 agtattagtg agaatatggg aatatatcga ttttgaggct aaagatcctc agctaaagag
      241 agaaatgcta aattactcga ttccattaat tgcaactgtt gttggatggt ggattaatag
      301 tacagctgat aggtatgtgg tttcctttat agtaggtgtc tcggcgaatg gcttattgtc
      361 tgtggcatat aaaatacctc aaataattaa tactttgcag ggaatattta tacaggcctg
      421 gcaaatttct gcaattaaag aatatggaga taatgagact gcaaaatttt atggaaatac
      481 atttgttatg attaatttgt taatgtgcat aacctgttca agtcttatca tattaactag
      541 accgttttca catattcttt atgcgaaaga cttttatgaa gcgtggaaat atgttccatt
      601 tttattaata gctaatgttt taaattgtgc atcaggtctg ttaggaccta tcttatcagc
      661 aaaaaaagat tctaaatcca tgatgtggtc tgcaatcata ggggcattag ttaatgtgat
      721 tatgagtgta gtccttgttt ataagatagg ggttcaaggt gccacaatag ccactgttat
      781 atgttcatat gtaatatatg ttgttcgtaa acgagctgtg ggaaaagata tttttataaa
      841 gcattatcat attgttgtgg aatcttgggg att
//
LOCUS       DAHUWM010000400          855 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.413, whole genome shotgun sequence.
ACCESSION   DAHUWM010000400 DAHUWM010000000
VERSION     DAHUWM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.413"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..290)
                     /locus_tag="NK788_002934"
     CDS             complement(<1..290)
                     /locus_tag="NK788_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCH6771200.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSES"
     gene            complement(290..>855)
                     /locus_tag="NK788_002935"
     CDS             complement(290..>855)
                     /locus_tag="NK788_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCH6771201.1"
                     /translation="SYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWII
                     DTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIG
                     EKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTL
                     LEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          290 a          151 c           96 g          318 t
ORIGIN      
        1 atactctcag aaaaagcagg tttttctaaa aatatgtaat caactttttc aacaattaca
       61 tttagtgttt ccagatgctt aaaagtcgga tttgtaataa aagctatatc ataatgtgta
      121 tccaaatcgt ctctattttg aatatacgtt atttcttcaa actctttttt tccactgcta
      181 ctataagctg ttatatcaaa attaatagaa ttcttttttc catagcttat tagatttttt
      241 agatgtctct ttccaattga ccctaaccca aaaaaaatta ctttcatcat taatttacct
      301 caatgtcgtt aattaagtta ataatatact tatctttttc aaaagtatat tggggttcca
      361 ttttcttttc caaaagcgtg aaaaattctt taatctcctc taaatatgca tcttcaataa
      421 tagtatgtga ataattatta tcatgcataa cctcatcgta caattgaata tctgtcatac
      481 tagaagtttc ttcattccat gaaaatagag aatccgggtt accttcccat tctattaaaa
      541 tcttttcacc aattatagtt aaatttcttt tagctactct tgaaacaaca tctacactta
      601 aagttccaac tataccagtt tcatgctcaa taataagagt gaaactgtcc gaataatcaa
      661 tatcaagatt ggataatttc tgggtcatta cagtaaacga tttaactttc ccaaatgtat
      721 ctataatcca aggtaattca atagcgaata tttctctaca cccattagtt tttttgtttg
      781 aaacaaaaaa attgcgataa tcttcccaag gatgccagtc gggtaaatac tgtcctacat
      841 ggtattgata agaaa
//
LOCUS       DAHUWM010000401          811 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.414, whole genome shotgun sequence.
ACCESSION   DAHUWM010000401 DAHUWM010000000
VERSION     DAHUWM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.414"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..672
                     /locus_tag="NK788_002936"
     CDS             <1..672
                     /locus_tag="NK788_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771202.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAHUWM010000402          806 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.415, whole genome shotgun sequence.
ACCESSION   DAHUWM010000402 DAHUWM010000000
VERSION     DAHUWM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.415"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>806
                     /locus_tag="NK788_002937"
     CDS             <1..>806
                     /locus_tag="NK788_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6771203.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL"
BASE COUNT          338 a           73 c          133 g          262 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttag
//
LOCUS       DAHUWM010000403          805 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.416, whole genome shotgun sequence.
ACCESSION   DAHUWM010000403 DAHUWM010000000
VERSION     DAHUWM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.416"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            6..>805
                     /locus_tag="NK788_002938"
     CDS             6..>805
                     /locus_tag="NK788_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HCH6771204.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DAHUWM010000404          789 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.417, whole genome shotgun sequence.
ACCESSION   DAHUWM010000404 DAHUWM010000000
VERSION     DAHUWM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.417"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>789
                     /locus_tag="NK788_002939"
     CDS             <1..>789
                     /locus_tag="NK788_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HCH6771205.1"
                     /translation="LNSFYQKSYWGYSLPNYISAVHNAHPNYASYLSYKNTLTIEAMN
                     EIFEMMDEEKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDKKILLIAPGKSS
                     VDEVERIKEFVTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNI
                     QASDVYLRTSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYG
                     EQSMEIITKFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRID"
BASE COUNT          315 a           84 c          152 g          238 t
ORIGIN      
        1 ctaaatagtt tctatcagaa gagttattgg ggatattcat taccaaacta tatttcagca
       61 gttcataatg ctcatcctaa ttatgcaagc tatctgtcat ataagaatac tttaacaatt
      121 gaagcaatga atgaaatatt cgaaatgatg gatgaagaaa aaaaggtatc ttatgataaa
      181 caatatatag aagatttata ctacaaatat atggaaactg ggaaaataca ggaggaatat
      241 atggaagaac tgagaaaatt tctctcagac aaaaaaattc tactgattgc accaggaaaa
      301 agttctgtag atgaggtaga aagaataaaa gagtttgtta ccaaagaaga agtcgtaata
      361 attagtgtaa attttgaata ttctcatata gatatagatt acgtattttt aagtaattta
      421 agacgttttc atgaattacc gaaagaaagc agaactaaat gtattgttac atcaaatata
      481 caagcatcgg atgtatattt acggacgagt tataaaaagt tgttatctga aagggaagtt
      541 gtaagagaca atgcaggatt aatggcaatt agatttttag ccgatatgaa tgtggatgaa
      601 atattcttag ctggatttga tggatattca cataatgaag atgagaatta tggtgagcaa
      661 tccatggaaa ttataacaaa atttgcatta ttagatgcaa tgaacgtgca aatgagagat
      721 gaattgtcag aattagctaa aaaagtaaag gtgacatttt taacggatcc acgttgggtt
      781 cgaattgat
//
LOCUS       DAHUWM010000405          786 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.418, whole genome shotgun sequence.
ACCESSION   DAHUWM010000405 DAHUWM010000000
VERSION     DAHUWM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.418"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            136..309
                     /locus_tag="NK788_002940"
     CDS             136..309
                     /locus_tag="NK788_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771206.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          261 a          127 c          100 g          298 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaa
//
LOCUS       DAHUWM010000406          755 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.419, whole genome shotgun sequence.
ACCESSION   DAHUWM010000406 DAHUWM010000000
VERSION     DAHUWM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.419"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            330..>755
                     /locus_tag="NK788_002941"
     CDS             330..>755
                     /locus_tag="NK788_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCH6771207.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEK"
BASE COUNT          288 a           83 c          125 g          259 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaa
//
LOCUS       DAHUWM010000407          755 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.420, whole genome shotgun sequence.
ACCESSION   DAHUWM010000407 DAHUWM010000000
VERSION     DAHUWM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.420"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(1..559)
                     /locus_tag="NK788_002942"
                     /pseudo
     CDS             complement(1..559)
                     /locus_tag="NK788_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          230 a          153 c          123 g          249 t
ORIGIN      
        1 gccgaagatc gtcacatccg aggaggctca ttgtcaagga caatcggaat agcatacgga
       61 aagaagttct gcaccttata aaatttgtca aaaaaactgt tgccattcca ccagacactt
      121 gcttaaagcg aaaaatttct tcaatgtcca tgcctaattc tttggatatt ctcttgtctg
      181 accatccatt ttttactaat ctttggatga caatttccat tcctcttatt tcatgggtcc
      241 ctcttgcacg attgtgtcga attgttgaag ccatacgttc ttatatgggt ttatcaataa
      301 ctgttaccgg tacaactttt aagttgagtc tttccttagc aacacgatac ctatgaagca
      361 catcaaccaa gatatacttc tcttcttttt ccttgtaata acaaactaat ggttgagtaa
      421 agccatcttc aatgatagaa agctccaata agttcatttc cggagacgct actcgattgg
      481 gattataatc gttcgcctgt attttggtaa ttggaacaag ccgaacgtcc atcacaggga
      541 aagttatgtc tttattcata cgcttgtata ccctactttc ttaaaaagta gggtatactc
      601 aagaatccga ttaattatct tctcaatttg gataatatat ctcatcagtc ttcttgtcac
      661 tttatcaaca tgattttgct gcaaaatggg ataccgacag tactggactg gtaatcagga
      721 taaaagcaaa aataattaat ttgtatccta aaaaa
//
LOCUS       DAHUWM010000408          752 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.421, whole genome shotgun sequence.
ACCESSION   DAHUWM010000408 DAHUWM010000000
VERSION     DAHUWM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.421"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>752
                     /locus_tag="NK788_002943"
     CDS             <1..>752
                     /locus_tag="NK788_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCH6771208.1"
                     /translation="FKRSFMISVDLLEEIEERFCIHFNEDEIAYVTLHIQSLLDRYKP
                     DKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPI
                     ENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNV
                     LNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPE
                     GIDWDGTLVHSVFFFALNEKVKPELNKIYETF"
BASE COUNT          290 a           96 c          137 g          229 t
ORIGIN      
        1 ttttaaaagg tcatttatga taagtgtaga tttattagag gaaatcgaag agagattctg
       61 tatacatttt aatgaagatg aaatcgctta cgtaacactc catattcaat cacttttgga
      121 tcgttacaaa cctgacaaaa cgaaagtgat attagtttgt agtagcggat atggaacatc
      181 aaaattatta gagcaaagaa taaataacgg ttttgcagat atggttgaga tagaagatgt
      241 actaagcatc aatgaactac aagatagaaa agtcactgat gaacttgtta taagcacgat
      301 tccgattgaa aatacaaatt ttcctgttat tgttgttagt cctttgatgt taggggcaga
      361 tgtcagaaaa gtaaaacagt atctacagtt aaatgaacaa acagaagatg cttttctaaa
      421 tttattagat aaacgatttt gcttttttac ccaccaaata aaggataatc aagtgaacgt
      481 tttaaatata attaccaaaa aattggtaaa agaaaattac gcgaaagaag ggatactaga
      541 gggagcaatc aaaagagaaa aactatcttc aacagcgatg aatgattttg ctatgccgca
      601 tatcgaagta gattttataa aaaagccagt aatctgtgtc tacgttaatc cagaaggaat
      661 tgattgggat gggacacttg ttcattctgt tttctttttt gcacttaatg aaaaagtaaa
      721 acctgaattg aataaaattt atgaaacatt ca
//
LOCUS       DAHUWM010000409          749 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.422, whole genome shotgun sequence.
ACCESSION   DAHUWM010000409 DAHUWM010000000
VERSION     DAHUWM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.422"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(438..662)
                     /locus_tag="NK788_002944"
     CDS             complement(438..662)
                     /locus_tag="NK788_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771209.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          276 a          105 c          137 g          231 t
ORIGIN      
        1 gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta
       61 ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg
      121 gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct
      181 acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta
      241 ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga
      301 tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt cctcattatg
      361 ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa
      421 gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata
      481 aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt
      541 tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg
      601 atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc
      661 attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga
      721 aggaactgta cgttttttag aataaaaat
//
LOCUS       DAHUWM010000410          740 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.423, whole genome shotgun sequence.
ACCESSION   DAHUWM010000410 DAHUWM010000000
VERSION     DAHUWM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.423"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            132..344
                     /locus_tag="NK788_002945"
     CDS             132..344
                     /locus_tag="NK788_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771210.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>740
                     /locus_tag="NK788_002946"
     CDS             506..>740
                     /locus_tag="NK788_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCH6771211.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKL"
BASE COUNT          270 a          105 c          126 g          239 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa
//
LOCUS       DAHUWM010000411          738 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.424, whole genome shotgun sequence.
ACCESSION   DAHUWM010000411 DAHUWM010000000
VERSION     DAHUWM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.424"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..625)
                     /locus_tag="NK788_002947"
     CDS             complement(<1..625)
                     /locus_tag="NK788_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCH6771212.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
BASE COUNT          222 a          133 c           83 g          300 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaacta
//
LOCUS       DAHUWM010000412          736 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.425, whole genome shotgun sequence.
ACCESSION   DAHUWM010000412 DAHUWM010000000
VERSION     DAHUWM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.425"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..734
                     /locus_tag="NK788_002948"
     CDS             <1..734
                     /locus_tag="NK788_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCH6771213.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DAHUWM010000413          733 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.426, whole genome shotgun sequence.
ACCESSION   DAHUWM010000413 DAHUWM010000000
VERSION     DAHUWM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.426"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>733
                     /locus_tag="NK788_002949"
     rRNA            <1..>733
                     /locus_tag="NK788_002949"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAHUWM010000414          731 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.427, whole genome shotgun sequence.
ACCESSION   DAHUWM010000414 DAHUWM010000000
VERSION     DAHUWM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.427"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            7..624
                     /locus_tag="NK788_002950"
     CDS             7..624
                     /locus_tag="NK788_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771214.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          274 a           93 c          136 g          228 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag t
//
LOCUS       DAHUWM010000415          720 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.428, whole genome shotgun sequence.
ACCESSION   DAHUWM010000415 DAHUWM010000000
VERSION     DAHUWM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.428"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..575)
                     /locus_tag="NK788_002951"
     CDS             complement(<1..575)
                     /locus_tag="NK788_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCH6771215.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKP"
BASE COUNT          212 a          162 c          109 g          237 t
ORIGIN      
        1 ataggcttta cactcaatag actgccaaga gtagcacttg ctttggaaat cctaccgcct
       61 cgttctagat atttcagatc gtctactgtt acccatgaat ggactttcaa ataattactt
      121 tccagccact ctagggtttc ttcgatgctt ttaccttctt ttttcaatct tacagcttcc
      181 aatactaaaa gcccttcccc aatacttgca gcttttgtat caaaaacata gatctcaggg
      241 tcttcatatt cttccttcag caattcaacc gcttgaagcg cactactgta agagccgctt
      301 aatccactgg aaaaagccac atataaaaca ggaatctgtt tttccgcata agaacggaaa
      361 aattctaaat atcggccaac attgatttgt gatgtcgtag gcatcgcacc atttttcagc
      421 tgattcatgt agcggtcata attgaatgtc tttccgaggt catctactaa ctcttgatca
      481 ttcaattgga gattcattga taaaaaatca actttttctt ttgctaatac atggtatggc
      541 agatcacaac aagaatctgt tacaatttga tacacatttt tcaacccctt ctcgttacat
      601 tttaccctta tcgtctatta tagcaaatct cttgcgctag acacacttta accggtttat
      661 tttcgcaagt tctaatcaaa ataagaggtt gtgacagaag tggtcagctt caagaaataa
//
LOCUS       DAHUWM010000416          714 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.429, whole genome shotgun sequence.
ACCESSION   DAHUWM010000416 DAHUWM010000000
VERSION     DAHUWM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.429"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            28..696
                     /locus_tag="NK788_002952"
     CDS             28..696
                     /locus_tag="NK788_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCH6771216.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          190 a          124 c          130 g          270 t
ORIGIN      
        1 aaattttcaa aagaaaagag gctagaaatg aaaaaattaa atttatttac agaattgtgc
       61 ggaatcattt taggcgcagg aatctacgga ttagctgtga caggaatcaa tattccatct
      121 aagctggcag atggtggggt tactggtatt gctttgcttt tgcataatct ttttggattc
      181 gctccttctg tcacttcact gctttttaat ttacctttat tgcttatctc attgtttctt
      241 tttggaaaac acgcttttct ccggacgatc gtcggaacat ttgcgctagt tttcttttta
      301 catttatggg agagccttca tatacatttt gctgtagata gtctactgat caatagtttg
      361 atgacaggta ttctttctgg cattggttgc ggtctcgtct ttcgattcgg cggaagcaca
      421 ggcggaacag atatcattta tcaagccatt gaaaaatatt ttcatattaa gattggcaaa
      481 agtcttttct tgattacttt tggtatttta gttgtctcct tgctttattt ggatctcaca
      541 cattttctgt atacattact tagctgttct attctatctt atacattgaa taaagtgaag
      601 tatctggaaa ttggatctgt tttgccccaa ttggaagcaa aacagactac agaaacattt
      661 gaaccacttg atcctcttga acagttagag cactgattct tatttttcac tact
//
LOCUS       DAHUWM010000417          714 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.430, whole genome shotgun sequence.
ACCESSION   DAHUWM010000417 DAHUWM010000000
VERSION     DAHUWM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.430"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            55..411
                     /locus_tag="NK788_002953"
     CDS             55..411
                     /locus_tag="NK788_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCH6771217.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            401..667
                     /locus_tag="NK788_002954"
     CDS             401..667
                     /locus_tag="NK788_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCH6771218.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          287 a           98 c          146 g          183 t
ORIGIN      
        1 cgaattttta tgttatatac tataatataa aatcaatata tgatataatt gggtatggaa
       61 aaaatatctt ttgaaacatt taaaagacct aatggtcatg atgaattttt agaatggttg
      121 gaaacattac caaaaaaaga tagtgcgaag cttttacgta caatagaaga aacagaaaag
      181 aatgggatgc tagtagctca acggttgaaa tgggtgaaaa aattagacac taatttgtac
      241 gaattaagaa gtaaggtagg atcaaacatc caaagagcta tctatttcca tgtggtcaat
      301 ggaaggtatg tcattaccca tggatttact aaaaaaacgg agaaaacacc aataagtgaa
      361 cttaaacatg caaaagaact tagagaggag tggttcacaa atgatgatta aaaaattgat
      421 tgaagaaaag caaaaagaca aagattttgc agaagcatat gaccgtgaag gaagaaaact
      481 cgaaactgcg gttgctcttt atcatgcacg ggaggaagca ggcctaactc aagcggagct
      541 tgcacaaaaa gcacatacaa ctcaatctac tattgctcga attgagagag gggataacgt
      601 atctttcgat aaacttgatg atatcgctag ggcattaggc aaaaagctgg tagtagaatt
      661 tgcttaaaag cgagttgaaa atatacctgt aataggctcc taactaatca atta
//
LOCUS       DAHUWM010000418          710 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.431, whole genome shotgun sequence.
ACCESSION   DAHUWM010000418 DAHUWM010000000
VERSION     DAHUWM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.431"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..338)
                     /locus_tag="NK788_002955"
     CDS             complement(<1..338)
                     /locus_tag="NK788_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HCH6771219.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSIT"
     gene            complement(341..>710)
                     /locus_tag="NK788_002956"
     CDS             complement(341..>710)
                     /locus_tag="NK788_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HCH6771220.1"
                     /translation="SHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKID
                     FGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNE
                     VSKRYQRGIKNLVKSYLKMN"
BASE COUNT          204 a          136 c           88 g          282 t
ORIGIN      
        1 ttagtaatgg aattttttgt cagcttatca ataagagatc caaactcact gtcaaaagtg
       61 tcctccttat taatacaagc tctgagctct ttaacataat tattgaaatc cctatttggt
      121 tgaatagtag cctctattcc tgtaatatca gaaaaaattt cgcgcgagga actacttctc
      181 tcagaataga gattttgata tgatttattc attcttgaca ttgctgagtc aacctcaact
      241 tggtaatctt taagctttat ttcttgttga ataatttctt ccaatcgctc ttcagttttt
      301 gataaagatt gatataaaga atcaaggttt tgtaacattt ttaattcatc ttgagataac
      361 tcttcactaa gtttttgata cctctttgat acctctttga aacctcatta tagttaactt
      421 tccaatctga tttattcagc tctgctctat aactatcaat ttcatctgac atttcattaa
      481 ttattgtttg aatcttagcc cgattggtca ctaaattctt aatcaattct ccgcggtgtt
      541 catcaatttc gtctgtaact tttataccat tgccaaagtc aattttgtca acactatcag
      601 ttagattttt gggaatttcc ttaagtgcct ccacatcacg tttcagttcc ctataaatat
      661 ctgtctcttt tgaatactca tcataaacat ctttataact cttatgagat
//
LOCUS       DAHUWM010000419          695 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.432, whole genome shotgun sequence.
ACCESSION   DAHUWM010000419 DAHUWM010000000
VERSION     DAHUWM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.432"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(53..628)
                     /locus_tag="NK788_002957"
     CDS             complement(53..628)
                     /locus_tag="NK788_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCH6771221.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DAHUWM010000420          695 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.433, whole genome shotgun sequence.
ACCESSION   DAHUWM010000420 DAHUWM010000000
VERSION     DAHUWM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.433"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            21..>695
                     /locus_tag="NK788_002958"
     CDS             21..>695
                     /locus_tag="NK788_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCH6771222.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENL"
BASE COUNT          264 a           76 c          137 g          218 t
ORIGIN      
        1 tcatttagaa ggagaaagtt atgttagaag tgaaaaagct taatgtttgt tataacgatt
       61 ttttagcatt gaacgagatt tctttaaaaa tacaagaagg tgctttaaca ggtattattg
      121 gtccaaatgg tgctggaaaa tccacattgc ttaaagcaat gttaaatatt atccctcatc
      181 agggagaggt ctccattaat aatgaagata tcaataaaaa actggtgaag attgcatatg
      241 tagagcaaaa agctgatatt gattttactt ttccaataaa agtaaaagaa tgtgtatcta
      301 tgggaacata tgccggaatg aaagtttttc aaagaattaa aaatgcagaa tggcaaagag
      361 tttcaaaggc tttggaaaag gtcgatatgg gaaaatattc taatcatcaa ataggagaat
      421 tatctggagg acaatttcaa cgtgtacttt tagcaagatg tcttgctcaa aatgcggatt
      481 tcatattttt agatgaacca tttgtaggga tagacttagt tagtgaacga ataattatgg
      541 atacgttaaa aaaattgaaa catcagggaa aaacagtgct aattgtgcat catgatttaa
      601 gcaaggtaaa gaagtatttt gataatatta ttcttttgaa tcgaaatcta attgctcacg
      661 gaagtgtaga atctgtattt aatgaagaaa attta
//
LOCUS       DAHUWM010000421          690 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.434, whole genome shotgun sequence.
ACCESSION   DAHUWM010000421 DAHUWM010000000
VERSION     DAHUWM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.434"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            67..408
                     /locus_tag="NK788_002959"
     CDS             67..408
                     /locus_tag="NK788_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771223.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            401..>690
                     /locus_tag="NK788_002960"
     CDS             401..>690
                     /locus_tag="NK788_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCH6771224.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          260 a          100 c          137 g          193 t
ORIGIN      
        1 aaataaaaaa gtgactcagc cggcaagcat agagtcacaa aacatatcta aggagaattt
       61 tagcatatga ataaagaact ttccacttta gatcaatatt tgactgattc tgaatggggc
      121 aagtcgaata tcaaggaaac aaataatcga aaaatcagac gaaatctttt gacgaacgaa
      181 gaactatcat gtgatcaaga tgacttggga aattttgtga gtatttggga tcatgtttac
      241 cttatccatc tatcaaaaca ttcaaataaa cctgaataca tctatgtcat cgaagatggc
      301 ttgattgatg cgctagaaga gtacaacaga gataacttga ttgatatctc ttattacgga
      361 tcaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac
      421 aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa
      481 ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc
      541 aacggtaact attaatgagt ataaacacta cagggttgtt tctggaactc atcaagactt
      601 tcttgttgag gaatataaac cttttcttat ggatgaaaca gggacttatg tatctgtctc
      661 agtaacggtt aaaggacata cggaaacaga
//
LOCUS       DAHUWM010000422          689 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.435, whole genome shotgun sequence.
ACCESSION   DAHUWM010000422 DAHUWM010000000
VERSION     DAHUWM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.435"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(204..353)
                     /locus_tag="NK788_002961"
     CDS             complement(204..353)
                     /locus_tag="NK788_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771225.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          227 a          112 c          103 g          247 t
ORIGIN      
        1 tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt
       61 ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag
      121 acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt
      181 cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg
      241 attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta
      301 gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc
      361 atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc
      421 aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta
      481 ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg
      541 ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa taacttagca
      601 atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg
      661 tctaagatcg tgttaaaaaa tagttatct
//
LOCUS       DAHUWM010000423          688 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.436, whole genome shotgun sequence.
ACCESSION   DAHUWM010000423 DAHUWM010000000
VERSION     DAHUWM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.436"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>688
                     /locus_tag="NK788_002962"
     CDS             <1..>688
                     /locus_tag="NK788_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCH6771226.1"
                     /translation="FCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVAKE
                     LFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFN
                     YNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNED
                     YTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWR
                     AGLYKVFSLDL"
BASE COUNT          243 a           89 c          124 g          232 t
ORIGIN      
        1 tttctgtgga ggagaaactt tactgagaaa aaaaatagtt ttatcttgtg ccactttatt
       61 aaaagaggca ggtattccta atattgctat tgttactaat ggatcattag taactcagga
      121 tgttgcaaaa gaattattca aagcaggtat taatcgattt caagtaagtt tagatggaaa
      181 aaatgctagt agccatgaag cacttagatt atatccaaac agttttgaaa aggcaattaa
      241 tgctatcaaa ttatttagag aattgtccga agaagtggat gttgcttttt gtcctactag
      301 ttttaattac aatcaattac gagaagtata tcaactagta aatactctta aagtgaagtc
      361 tttaagaatt caaccattta tgcttagtgg taggggaatg caaaataggg aaatagtgcc
      421 aactgtttct caatatagaa aactagtttc tgatattaac tatattaaaa gaaatgaaga
      481 ttataccaag atagattggg gagatccaat tgatcatatc atccgacaca gaactttatt
      541 gaaagatttt tcgaattttg tttctattag gtcagacgga ggattaatgg catcacctta
      601 cttgccaatt caagttggaa atattaaatg tcacagtctt tctaaatatt ggcgagcagg
      661 actttataaa gttttttctt tagattta
//
LOCUS       DAHUWM010000424          681 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.437, whole genome shotgun sequence.
ACCESSION   DAHUWM010000424 DAHUWM010000000
VERSION     DAHUWM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.437"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            23..>681
                     /locus_tag="NK788_002963"
     CDS             23..>681
                     /locus_tag="NK788_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCH6771227.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPKYF
                     K"
BASE COUNT          261 a          106 c          117 g          197 t
ORIGIN      
        1 ttgagaggga ttaaatatga caattaaaac tagtctccaa aatcaagctt atcaatctat
       61 tcgtcagcaa attatctacg ctgacttaga acctggaaaa aaagtatcag aaaaaaaatt
      121 ggaagaaacg ctttctatcg gtcgtactcc aatacgtgaa gcattgattc aactccgtca
      181 tcaaggactt gttgatacaa ttcctcaaag tggaaattat atctcattaa tcgacttaga
      241 tttagcaaaa aatgctcgtt ttgtcagaga acatttagaa cgacaaatta tgctagagtg
      301 ttgtgccaag atgaataatc aaaagaaaca agttttagaa actataattg cagaacaaga
      361 aaaagctgtt agtcaaaaga atgctcgtag tttttttcat aatgataatt tatttcataa
      421 agcttgtttt gaaattgcag aacgggaaga agtatggcaa tggttggaag ataataatac
      481 gcacttggaa cgatttcgtt ggttacgagt aaaaacggaa ggattaaaat gggaaaaaat
      541 tatggaacaa catcatcaac tatttaaagc cttgaccatg caagaccctg aagaagctaa
      601 cttcttaaca tccttgcatc ttcatttaat gcttgatgaa caaaatgttg tattgactac
      661 tcacccaaaa tattttaaaa a
//
LOCUS       DAHUWM010000425          666 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.438, whole genome shotgun sequence.
ACCESSION   DAHUWM010000425 DAHUWM010000000
VERSION     DAHUWM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.438"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            58..424
                     /gene="ssrA"
                     /locus_tag="NK788_002964"
     tmRNA           58..424
                     /gene="ssrA"
                     /locus_tag="NK788_002964"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          215 a          116 c          134 g          201 t
ORIGIN      
        1 ccaatatatg ctatactaat tttgtatgaa aaatacacgt aacccttgtt agctaacggg
       61 gacgttacgg attcgacagg cacagtcgag cttgaattgc gtttcgtagg ttacgtctac
      121 gtaaaaacgt tacagttaaa tataactgct aaaaacgaaa acaactctta cgctttagct
      181 gcctaaaaac agttagcgta gatcctctcg gcatcgccca tgtgctcgag taagggtcct
      241 aactttagtg ggatacgttt caactttccg tctgtaagtt gaaaaagaga acatcagact
      301 agcgatacag aatgcctgtc actcggcaag ctgtaaagtg aatccttaaa tgagttgact
      361 atgaacgtag atttttaagt ggcgatgtgt ttggacgcgg gttcgactcc cgccgtctcc
      421 atatatgtat ggttgagaca gataaacatg tatgaaaaac gttgttaaat caacgttttt
      481 ttattttggt caaggagaga aaagtagaga aatacaaaat tttttgcatt gattttgcat
      541 tcaaaaaaac attcaaacat ttagaaatcc ccaatacgta ttcaggaaaa tctattttaa
      601 ggtttacaaa aagaactttt gttcgtacac tctttttgag gtgaactttt atgctgatgg
      661 aaggaa
//
LOCUS       DAHUWM010000426          664 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.439, whole genome shotgun sequence.
ACCESSION   DAHUWM010000426 DAHUWM010000000
VERSION     DAHUWM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.439"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            122..>664
                     /locus_tag="NK788_002965"
     CDS             122..>664
                     /locus_tag="NK788_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCH6771228.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLD"
BASE COUNT          258 a           65 c          145 g          196 t
ORIGIN      
        1 tactttactg tttaagttga catggaaaca atcttttaaa aagcaaaaag atgataaagt
       61 tactttttat gggatgttgg tagaaaacaa attattataa atattagagg tataaaaagt
      121 tatgaaacac aaggtaagtg tgatagtacc aatttacaat gttgaaaaat atttaagaaa
      181 atgtgtggat agtttattga gtcagacatt aaaagagata gaaatcgtat tagttgatga
      241 tggttcgcca gatgcttcgg gggaaatagc agatgagtat caaaaaaaat attctaatgt
      301 aaaaacaata catagagaaa atggggggtt aggaccggca agaaatacag gaattgagaa
      361 tgcgacagga gaatatgtgg cttttcttga ttcagatgat tgggtagaaa gtgatatgta
      421 tgaaaaatta tacttagttg cgtccaaaga aaatgctgat atagtagtta gtggacattg
      481 tgattttgcg aatgacaaag ctatggtgaa aaagcaacat cctttgagtg ggaatgtata
      541 tgatacgcct aatagtatta aaaatgtacg aaaaaattta tttggacata gtccaaaaga
      601 tcaagaagta gaggccttcc cgatgtcagt atgtatgtct ttgtataata aaaatctgct
      661 agat
//
LOCUS       DAHUWM010000427          660 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.440, whole genome shotgun sequence.
ACCESSION   DAHUWM010000427 DAHUWM010000000
VERSION     DAHUWM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.440"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>660
                     /locus_tag="NK788_002966"
     CDS             <1..>660
                     /locus_tag="NK788_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HCH6771229.1"
                     /translation="LTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQI
                     QSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQS
                     CPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHY
                     PLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIAND
                     IL"
BASE COUNT          246 a           92 c          116 g          206 t
ORIGIN      
        1 ttaactgtaa acagtaacgt tgcacgtaat attgctagta atataagggc gatttatgtg
       61 gatgaatttc aagatactag agaattacaa tacaatgtaa tagctaaatt acttcaaaac
      121 aaccctcaaa ttcagtcaat gtttgttgga gatattgacc aagcaattta tggatcatta
      181 gatggtatag ctaaatcagt tgaagaacta gaaatgctaa ctagtcacac ttttcaatca
      241 aagacgctac atggctgtta caggtctaat cagagattag tagacttcta ctctaatttt
      301 cagagttgtc cttacgagat tgaaagcaga gggaataata aaaacgaccg tggttttata
      361 agttatgatt gcaaagttac taaggaggat ttacccaaca taataaaaaa tataataaag
      421 gaaaagattg aaagcggcat tcatgaaaaa gatatttgtg tacttgctcc tcaacactat
      481 cctctgtttt cactgggtga gtatttaaag aaagaattac cgtattgcaa ttttgatgct
      541 ataaatatat caccaattaa ggtaaataat catagtttat ttttcaagtt agcaattttg
      601 tactttactg aatcaggtga aaaggttcga agaagaaagc taattgcaaa cgatatttta
//
LOCUS       DAHUWM010000428          646 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.441, whole genome shotgun sequence.
ACCESSION   DAHUWM010000428 DAHUWM010000000
VERSION     DAHUWM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.441"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            130..348
                     /locus_tag="NK788_002967"
     CDS             130..348
                     /locus_tag="NK788_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771230.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            419..>646
                     /locus_tag="NK788_002968"
     CDS             419..>646
                     /locus_tag="NK788_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771231.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEK"
BASE COUNT          254 a           74 c          132 g          186 t
ORIGIN      
        1 gacatattaa atggcttaat gaagatatag aaattgatat tctcgatgaa tacaaacatg
       61 atattttaat ttaggtttat cattggaaag tttagataaa caaagtactt ttagaaagga
      121 gggaaaaaaa tgatttctga acctcaaatg aataatatgt atgaaagccc aactataatg
      181 ggagctggtg aagctgcgcc aagagcttgt tcagcaatta tggtgtgtgt gttattagca
      241 tctgcaggag taggtatcca agcaggagcg gtgtatactt atgctgtagt tgctacagta
      301 gctgtagctg cagccgcagt ttggaaaaca gtaggattag ttggttagga attaatagga
      361 gactaactgt ctcctattga ttccaatgat taagctaagg aggaatttta agaaaataat
      421 gaaatacaat aatttaaaac cgaaattact taatgagaac atagaatata taagagaaga
      481 aaattatgga tatttaacca tagttcccaa aaatggaact gaattgaaag ataacgtttt
      541 caatccaaca gtaagatata tattggctaa ctgtactgga aaaaacagtg taaaaaaaat
      601 agcgaatggt ttaaaaaaga agtttcctag tttagaatat gaaaaa
//
LOCUS       DAHUWM010000429          639 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.442, whole genome shotgun sequence.
ACCESSION   DAHUWM010000429 DAHUWM010000000
VERSION     DAHUWM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.442"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(323..580)
                     /locus_tag="NK788_002969"
     CDS             complement(323..580)
                     /locus_tag="NK788_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCH6771232.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          194 a          127 c           88 g          230 t
ORIGIN      
        1 ccaataaata atttttatat tctgacgcat ccattttact acggagatta tctgccgcac
       61 taaataactt ctgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag
      121 ttgctatata ccctcttatt ttagcatact cagttttaga aaaagcaaaa aaataaagaa
      181 acttattctt gataacaagc tacgtgacgt atatccctaa cacaaaaata gccatatcct
      241 ttggtttgga tacggctatt tgatattact cattcaatta tcaaatcaca gtaggtgtga
      301 atctcatcgg gatcggcaat atctacttct gattaggatc aaaggtgatt ttctcaataa
      361 aggtgtgata caaagcttta acttgcttct tctcactctg gataagaaag caatccaaac
      421 tagtcaatat ctgttggaca tctttgactt gaatcggttc cccttgttgt tgaaagatcc
      481 ctaagatttc attctctcga atttgtaact gtcttcgttt ttcagtcaat tcatttaaac
      541 gatcttttaa catcggaaac aattcgggac tatcttccaa agcagcttcc tgtttttgct
      601 ctaatagtta gattttgcgt accattttct tcttttatt
//
LOCUS       DAHUWM010000430          618 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.444, whole genome shotgun sequence.
ACCESSION   DAHUWM010000430 DAHUWM010000000
VERSION     DAHUWM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.444"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            60..215
                     /locus_tag="NK788_002970"
     CDS             60..215
                     /locus_tag="NK788_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCH6771233.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            282..>618
                     /locus_tag="NK788_002971"
     CDS             282..>618
                     /locus_tag="NK788_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCH6771234.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
BASE COUNT          213 a           83 c          138 g          184 t
ORIGIN      
        1 ccttagttac ttttttgtta ctatggtttt taagaaaaag tcgttggagg atacgaatta
       61 tgatcagtaa tgaaaaaaag agagtcgttg taacgcttac taaggaagtt gcagataatc
      121 ttgataaaat tgcgaaacaa atgggtctta gtaagtctgg tcttattaca atttgggtta
      181 atgctaacgg aaaggaaaat gagcaaaaaa aatagcaaaa gcattagtgt tggcgcactg
      241 cttttgctgt tagactgttg ttcaattaag gtaattatag catgacgagt agaaaaacta
      301 aagatgaacg cacaaggaat tggacgtttg tagtctatcc tgaatcggct cctgaaaatt
      361 ggagggagtt tttagatgag ttacatgttc cttgggttga gagtccactt catgataaag
      421 atgtgaatcc tgacggtgaa ttgaaaaagc ctcattggca tgttttgata atgtttagcg
      481 gtaaaaagac gtatgatcaa atacgtgaga taactcaaaa gcttcggtct ccaaacccgc
      541 aaaaatgtgc taatgcaaaa ggtatggtgc gatattttgc tcatatggat aatccagaaa
      601 aatttcaata tgctaaaa
//
LOCUS       DAHUWM010000431          604 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.445, whole genome shotgun sequence.
ACCESSION   DAHUWM010000431 DAHUWM010000000
VERSION     DAHUWM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.445"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            35..>604
                     /locus_tag="NK788_002972"
     CDS             35..>604
                     /locus_tag="NK788_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCH6771235.1"
                     /translation="MGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKK
                     MFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIM
                     ILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGV
                     ARLLFNQAAIKSNSIITSWAVHVIFDFTAI"
BASE COUNT          178 a          101 c          105 g          220 t
ORIGIN      
        1 tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc actattgtcg ttgtactcgg
       61 tttgttaggg ttgctatttg cgccatccat tattagcatt ttttctttaa ctatcactgg
      121 atttatagca tttacacttg tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc
      181 tgtcgctcct gtcaaaaata tagccaaata tttttttatc aatgtagtaa tcagtgccgc
      241 agtctcggta tttttgcaac aaattttgaa atggggttta acaggcaatc caataaatga
      301 ggctttcagc ccgttattat ttattatttt gcctatcatg atacttggag aagaattgtt
      361 ctcagtgtat tttctcgcta ttttttctag taagttcagc attcctgtcg ctagtttctt
      421 gtcagcaatc atctttggat ttatccatta cagtacttat gataatggaa atattttaca
      481 cactgtagca cacatccttt tgatacaagg ggtggcaaga ttgttattca accaagctgc
      541 cattaaaagc aactcgatca ttacaagttg ggcagttcat gtgatatttg atttcacagc
      601 tata
//
LOCUS       DAHUWM010000432          589 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.446, whole genome shotgun sequence.
ACCESSION   DAHUWM010000432 DAHUWM010000000
VERSION     DAHUWM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.446"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..>589)
                     /locus_tag="NK788_002973"
     CDS             complement(<1..>589)
                     /locus_tag="NK788_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCH6771236.1"
                     /translation="IFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKI
                     KWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQH
                     SSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELK
                     RAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFS"
BASE COUNT          217 a          104 c           74 g          194 t
ORIGIN      
        1 aaactaaaga tatttaacac atagaaagca tagatacata taaaagcaat tataccactt
       61 gatacaagta cttgaagaaa tccattatgc atgtttccat gtgctctttt tagttcattc
      121 acatctgtag tatcaagatc tacttttttc aaaaattttg catcgctatt cccatagaaa
      181 tcggtctctc cataaccaaa taacaaagtt ggtcttataa ctttaagcgc agctttccaa
      241 atcgtaaaac gaccagctga aatttcggag ttatcactag aatgctgtaa tattacttct
      301 ttttctggaa gtggagtttt atctgattca tttgactcta taacttctac ttttctatca
      361 atcgatacag gaattgttgc taaaaaatcc ttagcataat tagtaccaaa agaaaaatat
      421 accaatagaa ctaataatat gcttaccatc catttgattt ttacaggtag tacttctcta
      481 atgctctttc taaattttga attggcaaac aacaaaagaa ataatactaa aaaagaacaa
      541 attactacct gggcacctct agacgaagaa agtgtaatat aaaatattt
//
LOCUS       DAHUWM010000433          561 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.447, whole genome shotgun sequence.
ACCESSION   DAHUWM010000433 DAHUWM010000000
VERSION     DAHUWM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.447"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..435
                     /locus_tag="NK788_002974"
     CDS             <1..435
                     /locus_tag="NK788_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCH6771237.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          181 a          128 c          109 g          143 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat t
//
LOCUS       DAHUWM010000434          554 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.448, whole genome shotgun sequence.
ACCESSION   DAHUWM010000434 DAHUWM010000000
VERSION     DAHUWM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.448"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..376)
                     /locus_tag="NK788_002975"
     CDS             complement(<1..376)
                     /locus_tag="NK788_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6771238.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSV"
BASE COUNT          162 a          106 c           81 g          205 t
ORIGIN      
        1 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
       61 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      121 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      181 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      241 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      301 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      361 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      421 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      481 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      541 ctttctcgcg atca
//
LOCUS       DAHUWM010000435          537 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.449, whole genome shotgun sequence.
ACCESSION   DAHUWM010000435 DAHUWM010000000
VERSION     DAHUWM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.449"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            41..>537
                     /locus_tag="NK788_002976"
     CDS             41..>537
                     /locus_tag="NK788_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCH6771239.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          198 a           99 c           88 g          152 t
ORIGIN      
        1 aagttaacga caaccaattc acagaaaaga ggacttccct atgaatgatt ttactacaga
       61 aattgtgcaa actctagtca ctaaaggcga tttaaatgaa ttattccgtt cgcacttaga
      121 aaaagcgata aacacactcc tacggactga attaacggct tttttagatt acgaaaaata
      181 tgatcgcact ggttttaatt caggtaattc gagaaacggt tcttactttc gatcaatcaa
      241 aaccgaatat ggtgaattaa cattggaaat acctagagat cgtaatggtg agtttaaaca
      301 acaaacttta ccagcctaca aaagaacaaa cgatacattg gaaaccacta ttatccattt
      361 attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg atcgaaaaaa tgtacggtca
      421 tcactatact ccacaaacca tgtccaacat gactaaagtt ctgactgaag aagtaaatgc
      481 ctttaaatcc agagccttaa atgataagta tgtcgctatt tttatggacg ctactta
//
LOCUS       DAHUWM010000436          534 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.450, whole genome shotgun sequence.
ACCESSION   DAHUWM010000436 DAHUWM010000000
VERSION     DAHUWM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.450"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(14..367)
                     /gene="tnpB"
                     /locus_tag="NK788_002977"
     CDS             complement(14..367)
                     /gene="tnpB"
                     /locus_tag="NK788_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCH6771240.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(357..>534)
                     /locus_tag="NK788_002978"
     CDS             complement(357..>534)
                     /locus_tag="NK788_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771241.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          155 a          120 c           90 g          169 t
ORIGIN      
        1 ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga ggttgatcga
       61 tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga acttcgtctt
      121 tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga agaaaaccat
      181 caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat aaaaaaacgg
      241 catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga ccatcaattc
      301 cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga agtaaatcat
      361 aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt aataccgtta
      421 tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt ggcagccgcg
      481 ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac aatc
//
LOCUS       DAHUWM010000437          532 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.451, whole genome shotgun sequence.
ACCESSION   DAHUWM010000437 DAHUWM010000000
VERSION     DAHUWM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.451"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
BASE COUNT          163 a          110 c           75 g          184 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aa
//
LOCUS       DAHUWM010000438          525 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.452, whole genome shotgun sequence.
ACCESSION   DAHUWM010000438 DAHUWM010000000
VERSION     DAHUWM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.452"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            35..523
                     /locus_tag="NK788_002979"
     CDS             35..523
                     /locus_tag="NK788_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCH6771242.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          219 a           41 c          101 g          164 t
ORIGIN      
        1 agaattgtac tagtaggata aggatggatg gaaaatgaaa aaaactattt ttgttttagc
       61 gatttttata tgttttgggc tacttttggt aggctacttg ttgtttaatt ctaatgaaat
      121 gagaactatt tctacgaatg acttgttaaa aaaaagtgaa ttaaaggata aaaaaaaaga
      181 atctcttgcc tttataatgt ttactcaaga agagtgtgag tattgtatag caataaatcc
      241 aatcatagat agtgtaaata aggaaacttt gaataagata aagaaatatg atatatctgt
      301 agattttcaa tctaaacaaa agtgtaaaat taagtttgtt ccgactattg ttgcatatag
      361 taagaataaa gaagtgggga gaattgacaa tgaattagat aatttgtatc gaaaaaagca
      421 agatggttca attgtaatag acaagaaaaa aacaaagaca ttgcttatag attggatgaa
      481 aaaaatggat aaacaaggag aatatttgta tggagaagat taaac
//
LOCUS       DAHUWM010000439          514 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.453, whole genome shotgun sequence.
ACCESSION   DAHUWM010000439 DAHUWM010000000
VERSION     DAHUWM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.453"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(125..424)
                     /locus_tag="NK788_002980"
     CDS             complement(125..424)
                     /locus_tag="NK788_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCH6771243.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          161 a           99 c           61 g          193 t
ORIGIN      
        1 tataaagaaa catctgtttc tttatttaga ataaaaaaat acagactctg ttcaaataaa
       61 tgaacctagc ctgcattatg agattgacta tactttttaa ttcattgaga tgaataactc
      121 gtttttataa atgcccaaaa gtaatcggtg ccgaagccat tcctcgacct aattcttttt
      181 caaattttcc tattgttgat atctccatat aaaacggtga tactggttta gacaattctc
      241 ccctgattgc taacggacgt aaactacgtt ccaaattatt tatgacttta ggataatact
      301 gacctttttc taagcctgtc tttgcgtcta tcaaaagtat acgttccttt tctttgatct
      361 ctggatttaa aacgagatca tataatttta gtaccatgtt ttctatttct tcacttttag
      421 acatcacgat ttcctcccaa aaatatattt cttcccacta cattctataa acaaagtata
      481 cgctacatct agattttttt aaatcattac tgga
//
LOCUS       DAHUWM010000440          514 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.454, whole genome shotgun sequence.
ACCESSION   DAHUWM010000440 DAHUWM010000000
VERSION     DAHUWM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.454"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..132)
                     /locus_tag="NK788_002981"
     CDS             complement(<1..132)
                     /locus_tag="NK788_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCH6771244.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAHUWM010000441          510 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.455, whole genome shotgun sequence.
ACCESSION   DAHUWM010000441 DAHUWM010000000
VERSION     DAHUWM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.455"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            1..510
                     /locus_tag="NK788_002982"
                     /pseudo
     CDS             1..510
                     /locus_tag="NK788_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DAHUWM010000442          497 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.456, whole genome shotgun sequence.
ACCESSION   DAHUWM010000442 DAHUWM010000000
VERSION     DAHUWM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.456"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(27..425)
                     /gene="spxA"
                     /locus_tag="NK788_002983"
     CDS             complement(27..425)
                     /gene="spxA"
                     /locus_tag="NK788_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCH6771245.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          141 a          102 c           85 g          169 t
ORIGIN      
        1 tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat
       61 tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact
      121 tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca
      181 agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact
      241 ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt
      301 aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt
      361 tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt
      421 aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa
      481 taatctccca tctgttt
//
LOCUS       DAHUWM010000443          491 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.457, whole genome shotgun sequence.
ACCESSION   DAHUWM010000443 DAHUWM010000000
VERSION     DAHUWM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.457"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            54..>491
                     /locus_tag="NK788_002984"
     CDS             54..>491
                     /locus_tag="NK788_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HCH6771246.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYL"
BASE COUNT          196 a           68 c           81 g          146 t
ORIGIN      
        1 aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg tatatgccta
       61 acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa ccggcatcca
      121 ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa aatttaaaag
      181 aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata tcagtacctg
      241 atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt aataaataca
      301 tcagggatag gaataaccca gcattaaaaa aacctcttaa aacatttggc gaaaaagatg
      361 ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa gtattgaatg
      421 actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga agtattttag
      481 aggaatattt a
//
LOCUS       DAHUWM010000444          485 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.458, whole genome shotgun sequence.
ACCESSION   DAHUWM010000444 DAHUWM010000000
VERSION     DAHUWM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.458"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(62..259)
                     /locus_tag="NK788_002985"
     CDS             complement(62..259)
                     /locus_tag="NK788_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCH6771247.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAHUWM010000445          476 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.459, whole genome shotgun sequence.
ACCESSION   DAHUWM010000445 DAHUWM010000000
VERSION     DAHUWM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.459"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(108..>476)
                     /locus_tag="NK788_002986"
     CDS             complement(108..>476)
                     /locus_tag="NK788_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6771248.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQ
                     RLAQRILDNELGCANASEAV"
BASE COUNT          137 a          103 c           92 g          144 t
ORIGIN      
        1 gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt ccttgacaat
       61 gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta gactgcctcg
      121 ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaatct ctggttacgg
      181 taaccaaccg gtgtctcgta gccaagtgta ccgtgcaacc gaaggtggtt ccaccaattg
      241 acatagtcaa ataactccaa atccaattgt tgtaaggttt caaatgtgta ttgatagaca
      301 aattctactt tcaacgactt ataagttgat tcagctacgg cattatcaaa aggacagcct
      361 ttatgactca atgatcgatt gatgtcaaaa gttgttaata attcatcaat agcttggtta
      421 tcaaactctt ttccacgatc agtatgaaaa atctcaacct ctgtcagagg ttgttt
//
LOCUS       DAHUWM010000446          474 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.460, whole genome shotgun sequence.
ACCESSION   DAHUWM010000446 DAHUWM010000000
VERSION     DAHUWM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.460"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            5..>474
                     /locus_tag="NK788_002987"
     CDS             5..>474
                     /locus_tag="NK788_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCH6771249.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          165 a           92 c           73 g          144 t
ORIGIN      
        1 ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct attcattgaa
       61 aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg gtctagcacc
      121 tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc tggttatctt
      181 aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag cagcagaagc
      241 ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg taaatggaga
      301 aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg tcactaaagc
      361 aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa tcgaacgatt
      421 tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc ttta
//
LOCUS       DAHUWM010000447          462 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.461, whole genome shotgun sequence.
ACCESSION   DAHUWM010000447 DAHUWM010000000
VERSION     DAHUWM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.461"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..430)
                     /locus_tag="NK788_002988"
     CDS             complement(<1..430)
                     /locus_tag="NK788_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCH6771250.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAHUWM010000448          457 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.462, whole genome shotgun sequence.
ACCESSION   DAHUWM010000448 DAHUWM010000000
VERSION     DAHUWM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.462"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>457
                     /locus_tag="NK788_002989"
     CDS             <1..>457
                     /locus_tag="NK788_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771251.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          185 a           68 c          106 g           98 t
ORIGIN      
        1 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag
       61 ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag
      121 caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg
      181 ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata
      241 aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt
      301 atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag
      361 tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc
      421 gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DAHUWM010000449          439 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.463, whole genome shotgun sequence.
ACCESSION   DAHUWM010000449 DAHUWM010000000
VERSION     DAHUWM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.463"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
BASE COUNT          171 a           54 c           82 g          132 t
ORIGIN      
        1 aaattgttgg tagaaggtga tcaaaatggg agtaaacgaa atgatgaaag tcagattgag
       61 tttttaaatg ataccttcct cagaacttca aaacgaattc aaagattcgt tgacaaatct
      121 tgagcaagat catttaaaaa aatattttct cttcatttga cgaaaaatgt ttagaaatct
      181 tatataagtg aagataaagt aattggtgta attaattgta gagaatagcc tttgagcaat
      241 tgaagcgata caattattta gaaaatgaac ttcctccagc atactacatg attccttacg
      301 ggacgatgtt aaagaagaaa gcaagaaatt ttagaagtat tagtagataa actaatgggc
      361 ttttctcaat ttaagggagt agcccaaatg tttatcaaat atggtgcaga tacagtagca
      421 accatgttta cagaagaat
//
LOCUS       DAHUWM010000450          415 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.464, whole genome shotgun sequence.
ACCESSION   DAHUWM010000450 DAHUWM010000000
VERSION     DAHUWM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.464"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..339)
                     /locus_tag="NK788_002990"
     CDS             complement(<1..339)
                     /locus_tag="NK788_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCH6771252.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAHUWM010000451          410 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.465, whole genome shotgun sequence.
ACCESSION   DAHUWM010000451 DAHUWM010000000
VERSION     DAHUWM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.465"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..>410)
                     /locus_tag="NK788_002991"
     CDS             complement(<1..>410)
                     /locus_tag="NK788_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCH6771253.1"
                     /translation="KNKGIITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFD
                     IDIWSIRRPDYAHASVASGYFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLM
                     IDYMIVLAQRINHEIAQTFDKIPSNNPLCDELIK"
BASE COUNT          139 a           77 c           49 g          145 t
ORIGIN      
        1 ttttaatcaa ttcgtcacat aatggattgt tactaggtat tttatcaaat gtctgtgcta
       61 tttcatgatt tattctctgt gctaaaacaa tcatataatc tatcatcaaa taatccacca
      121 tcatatcatt atttttccaa taatttaaaa aaaagtctct aattatagca aacatccatc
      181 tagactcttg attacatgct aatgaataac ccgcaaaata tcctgatgct acggatgcgt
      241 gagcataatc aggcctccta atagaccaaa tatcgatatc aaaatactca tctggtattg
      301 gttttgtaca ataaaatgtg gcgtctaacc acaaacctcc atgttttgct agcaaagata
      361 atcttaataa atcagaataa tttgttctcg taatgattcc tttgtttttc
//
LOCUS       DAHUWM010000452          409 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.466, whole genome shotgun sequence.
ACCESSION   DAHUWM010000452 DAHUWM010000000
VERSION     DAHUWM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.466"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..379
                     /locus_tag="NK788_002992"
     CDS             <1..379
                     /locus_tag="NK788_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCH6771254.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAHUWM010000453          391 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.467, whole genome shotgun sequence.
ACCESSION   DAHUWM010000453 DAHUWM010000000
VERSION     DAHUWM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.467"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..139)
                     /locus_tag="NK788_002993"
     CDS             complement(<1..139)
                     /locus_tag="NK788_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCH6771255.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KA"
     gene            complement(158..319)
                     /locus_tag="NK788_002994"
     CDS             complement(158..319)
                     /locus_tag="NK788_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCH6771256.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          130 a           74 c           42 g          145 t
ORIGIN      
        1 aagccttttc tatttcattg cttgatttca ttattgttaa acaagaatac aagcaagaaa
       61 gaactatagt taatatgata aaaattatta aagttctatt tttctttctt gtaatatatg
      121 ctatagcatt ttttatcact ttcctaacta ctcctctcta ttttcttact ccataactaa
      181 ttgcatccac tggacaagca tttttacatc gtccacatct gatacactcc agatgattgc
      241 aattctcaac tgaatcttta ttcatttggc aaactttttt acattttcca caattaatac
      301 acttatcctt atcctgaatc ggctacacta gactagacag aaaaaataag gtgtgtagaa
      361 taataaaaaa cacactggag gatttttcat g
//
LOCUS       DAHUWM010000454          385 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.468, whole genome shotgun sequence.
ACCESSION   DAHUWM010000454 DAHUWM010000000
VERSION     DAHUWM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.468"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(71..316)
                     /locus_tag="NK788_002995"
     CDS             complement(71..316)
                     /locus_tag="NK788_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCH6771257.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT          124 a           83 c           59 g          119 t
ORIGIN      
        1 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
       61 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
      121 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
      181 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
      241 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
      301 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
      361 ttcaagttta ccataaaaaa aaaaa
//
LOCUS       DAHUWM010000455          376 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.469, whole genome shotgun sequence.
ACCESSION   DAHUWM010000455 DAHUWM010000000
VERSION     DAHUWM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.469"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            81..>376
                     /locus_tag="NK788_002996"
     CDS             81..>376
                     /locus_tag="NK788_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCH6771258.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEK"
BASE COUNT          137 a           53 c           84 g          102 t
ORIGIN      
        1 ttaaataaaa tcattcacgc caattcaaat atcatgtata atgaataaag ttacgaactt
       61 gatagtaagg aggagttgtt ttgtcaggaa acaaacaaca cccaagtaaa gataaaaaag
      121 aagccagtaa ggcagctaca gcagatataa tcgacaagcg gagaaatctg cgtaaaaaag
      181 aagataagat tgtgaggaag atcattttag tgattgctct tacattattg attattggcg
      241 gatttcttgg atttaccgtg tatcgttatg tagatagcgg gttgaagcct ttagataaat
      301 cagatgacca attagtccaa gtcgagatcc cgagtggctc ttctaacaag cagattggtg
      361 agattttgga gaagga
//
LOCUS       DAHUWM010000456          374 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.470, whole genome shotgun sequence.
ACCESSION   DAHUWM010000456 DAHUWM010000000
VERSION     DAHUWM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.470"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..88
                     /locus_tag="NK788_002997"
     CDS             <1..88
                     /locus_tag="NK788_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCH6771259.1"
                     /translation="FFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            89..>374
                     /locus_tag="NK788_002998"
     CDS             89..>374
                     /locus_tag="NK788_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCH6771260.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSF"
BASE COUNT          140 a           48 c           76 g          110 t
ORIGIN      
        1 tttctttgaa aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga
       61 agtattgaaa atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc
      121 tgggtctaag gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca
      181 tttcgcttta gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac
      241 ttgtttgaat acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt
      301 gaaaattgta gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat
      361 acgtgattca ttct
//
LOCUS       DAHUWM010000457          367 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.471, whole genome shotgun sequence.
ACCESSION   DAHUWM010000457 DAHUWM010000000
VERSION     DAHUWM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.471"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            45..239
                     /locus_tag="NK788_002999"
     CDS             45..239
                     /locus_tag="NK788_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771261.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          164 a           46 c           68 g           89 t
ORIGIN      
        1 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
       61 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      121 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      181 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      241 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      301 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      361 atgatta
//
LOCUS       DAHUWM010000458          331 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.472, whole genome shotgun sequence.
ACCESSION   DAHUWM010000458 DAHUWM010000000
VERSION     DAHUWM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.472"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..>331
                     /locus_tag="NK788_003000"
     CDS             <1..>331
                     /locus_tag="NK788_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HCH6771262.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAHUWM010000459          322 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.473, whole genome shotgun sequence.
ACCESSION   DAHUWM010000459 DAHUWM010000000
VERSION     DAHUWM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.473"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            46..>322
                     /locus_tag="NK788_003001"
     CDS             46..>322
                     /locus_tag="NK788_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCH6771263.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          102 a           58 c           62 g          100 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gt
//
LOCUS       DAHUWM010000460          321 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.474, whole genome shotgun sequence.
ACCESSION   DAHUWM010000460 DAHUWM010000000
VERSION     DAHUWM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.474"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(69..321)
                     /locus_tag="NK788_003002"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="NK788_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAHUWM010000461          311 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.475, whole genome shotgun sequence.
ACCESSION   DAHUWM010000461 DAHUWM010000000
VERSION     DAHUWM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.475"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NK788_003003"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NK788_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCH6771264.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           98 a           46 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc a
//
LOCUS       DAHUWM010000462          309 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.476, whole genome shotgun sequence.
ACCESSION   DAHUWM010000462 DAHUWM010000000
VERSION     DAHUWM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.476"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            51..>309
                     /locus_tag="NK788_003004"
     CDS             51..>309
                     /locus_tag="NK788_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCH6771265.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DAHUWM010000463          308 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.477, whole genome shotgun sequence.
ACCESSION   DAHUWM010000463 DAHUWM010000000
VERSION     DAHUWM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.477"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(76..>308)
                     /locus_tag="NK788_003005"
     CDS             complement(76..>308)
                     /locus_tag="NK788_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771266.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAHUWM010000464          307 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.denovo.478, whole genome shotgun sequence.
ACCESSION   DAHUWM010000464 DAHUWM010000000
VERSION     DAHUWM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.denovo.478"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            17..220
                     /locus_tag="NK788_003006"
     CDS             17..220
                     /locus_tag="NK788_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HCH6771267.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          116 a           37 c           68 g           86 t
ORIGIN      
        1 aaagaaaaag aaagtattga aacattgtgt tattctagga atattaggaa cttgtctagc
       61 tggcattggt acaggaatag acgttgacgc agctacttac tatggaaatg gtctttattg
      121 taacaaagaa aaatgttggg taaattgggg acaatcatgg tcagaagggt taaaacgttg
      181 gggagataat ctttttggta gttttagtgg aggacgataa tgacttcaat aaacattgat
      241 cacacaaaaa aagtcataca tgaattatac aatagtttaa tgacacgacc taatcagacg
      301 aaagcat
//
LOCUS       DAHUWM010000465        13091 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.076, whole genome shotgun sequence.
ACCESSION   DAHUWM010000465 DAHUWM010000000
VERSION     DAHUWM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.076"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            116..613
                     /gene="dfrG"
                     /locus_tag="NK788_003007"
     CDS             116..613
                     /gene="dfrG"
                     /locus_tag="NK788_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCH6771268.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(685..2637)
                     /locus_tag="NK788_003008"
     CDS             complement(685..2637)
                     /locus_tag="NK788_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCH6771269.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2644..2880)
                     /locus_tag="NK788_003009"
     CDS             complement(2644..2880)
                     /locus_tag="NK788_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771270.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3115..3918)
                     /locus_tag="NK788_003010"
     CDS             complement(3115..3918)
                     /locus_tag="NK788_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCH6771271.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(3934..4050)
                     /locus_tag="NK788_003011"
     CDS             complement(3934..4050)
                     /locus_tag="NK788_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCH6771272.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(4295..5224)
                     /locus_tag="NK788_003012"
     CDS             complement(4295..5224)
                     /locus_tag="NK788_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6771273.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(5241..6263)
                     /locus_tag="NK788_003013"
     CDS             complement(5241..6263)
                     /locus_tag="NK788_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCH6771274.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(6260..8287)
                     /locus_tag="NK788_003014"
     CDS             complement(6260..8287)
                     /locus_tag="NK788_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771275.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(8284..10737)
                     /locus_tag="NK788_003015"
     CDS             complement(8284..10737)
                     /locus_tag="NK788_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCH6771276.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(10721..11116)
                     /locus_tag="NK788_003016"
     CDS             complement(10721..11116)
                     /locus_tag="NK788_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCH6771277.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(11188..11826)
                     /locus_tag="NK788_003017"
     CDS             complement(11188..11826)
                     /locus_tag="NK788_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCH6771278.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(11882..12376)
                     /locus_tag="NK788_003018"
     CDS             complement(11882..12376)
                     /locus_tag="NK788_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCH6771279.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(12443..>13091)
                     /locus_tag="NK788_003019"
     CDS             complement(12443..>13091)
                     /locus_tag="NK788_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCH6771280.1"
                     /translation="VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDL
                     ITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGE
                     YQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVK
                     TTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
BASE COUNT         3888 a         2535 c         2167 g         4501 t
ORIGIN      
        1 aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg agaaaaggtt
       61 ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt tataaatgaa
      121 agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga atgacattcc
      181 ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac atccgataat
      241 attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa gaaatattat
      301 tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt caatagaaga
      361 tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag aacagattta
      421 taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc atgaattcga
      481 aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa
      541 agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg aaagaaaaaa
      601 cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag cacaagagct
      661 tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc aatcaaattg
      721 tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa
      781 agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag catacttcgg
      841 attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta atggtctttc
      901 aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat ggtcattaac
      961 atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg ttctacaatc
     1021 aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt tatcggcata
     1081 tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat gacttgttag
     1141 taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga atcctctgat
     1201 atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc attgttttct
     1261 gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc ttaattcttt
     1321 cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa tcattcgcca
     1381 ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag tataaggagc
     1441 atcaacattt aaaccatcga caaatctact cctattagta attttcccta attcgtgaag
     1501 ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat aagaccaatc
     1561 taacctatca tttttacgac taaaagtatt gagatggtct tctttgaatt ttcttgtatc
     1621 ttcatttaat tcatccattg aaatattcca atcataggca acatcagaac ggaacacata
     1681 tcttgataag acctcagcag atacaactat atacgtttgc atattaaggt cttcaaaccc
     1741 cgtatatttc tttgaacctt gtcctttctt acggacgtta tttaggtagt agtgcatctt
     1801 gtccgtatta agccacatct cgttaaattt ctttgtttga aggggttgtg tctcatatcg
     1861 ttctaaaaac tccaaacaac gcctatacag ccactccagc ttatgatagt aggttccaac
     1921 tccaattcct aaaatatcac aagtacgact gactgaaacc cttccaacaa ccatcttagc
     1981 gaacattgga agaatggtgt tcttttgttg gtgataagta gtggtttctt ctctttttgg
     2041 tagaacatta gtgaacttct tacacgcctt acattgatag cgttgagatt tgcccctact
     2101 tttccccctt ttgtaaaatt gcttcggctc attgaatggg gttgattctt cttccgagca
     2161 gccttcttta tgaaattgat aatctggctc aacatcttgg atgctattga tttctataag
     2221 gcgtttaatt tcttcaacaa cagaccaatt agataaaggg gttactgtac agttttgcga
     2281 tacacctctg ccaataggat tgtcattgca gtacatacct ttatgacctt tatctttgga
     2341 actaccagtc attttatatc gacttggttt cccttttaca tctttgaatt tgtgctgttc
     2401 tttcccaaaa ttacaacaaa acggattgat gcagtaatta tattgaatgg gatattcaga
     2461 accgttccat ttaaaagacg ttggcaagaa caacattttg ttataccttg ttgcaaatcg
     2521 ttttggagtt aataaatcat attgtttagc cctatctttt aattcttcgt ttgaaatcgg
     2581 aattttaaca tctacaattt catcttcctt cgtggctaat cttttaagct tggtcattgt
     2641 ctatcaaccc actctatctt tgatacccat tttttcaaca atccgcttat tacttcgctt
     2701 gttggcaatc cattcaataa acttcttatc gtctaaaatg ttcaataaga attcatccac
     2761 tgcttcgctt tcagtacgct ctgcatattc agcgtattgc ttaactatca cccttacttg
     2821 ctctgatatt tcccaatcca cagacttagc gttcttattt tttggctcaa gaaattgcat
     2881 aggaaaatgc accccttttt gcttagtaaa ttgtaaccat tttaacacac caatttgcat
     2941 tcctacaata attatttcac gttcgcattt ttatatacat tttaacttac attttgtggt
     3001 gtaaattaac aacaaacgtt gatttaacaa taaaaaacga gtatcaaatt cagttgcaat
     3061 ttgatactca tttaggttta cattttcact ttttgtactc caaaactgaa ctacttaata
     3121 tgcaagacgt tgtctttttt ttgtatattc aattttgaaa acatttccgc aaagttcaga
     3181 cggacctcga tgtgttgatg aataaaattt attagtaata acttctataa ggttatcaat
     3241 ccctatatta ctttttgcac ttccatgata aagagggaac agagaacaat tctgaaatct
     3301 tatgctttcc tcttgttcga gttccaatgc ttctaatgat ttaccggaca tatatttctc
     3361 taaaaggtca tcgtttccct ctattaccgt atcccattgt tcagattcgg taaagttcgt
     3421 cacacacata ttaggataca gttctacctt ctgtttgatt acaatttcgg cagaaagttt
     3481 ctctttaata tcctgataaa ccgttgataa atcaattcca ttttggtcaa tcttattgat
     3541 aaaaaagatt gtgggaatcc ccattttcct aagtgcatga aataatatac gagtttgtgc
     3601 ttgtacgcca tcttttgcag aaatcagtag aattgcccca tctaaaactg ataatgaacg
     3661 atatacttct gctaagaaat ccatatgtcc tggcgtgtct atgatgttca ccttcgtatt
     3721 ttcccactga aaagaggtta ttcctgtctg aattgtaatt cctctctgac gttctaaaag
     3781 cgtattatcc gtcctcgttg tacctttgtc cacgcttcct aattctgtaa tcgctccact
     3841 gttatataat aagctttctg ttaaggtagt ttttcctgca tcaacatgag ctaaaactcc
     3901 aatattaata attttcatgt gattttcctc cattcaaaaa cccaaaaggg cataaaaatc
     3961 ccagtgataa atacttttat cactgggatt tttatgcata accataggta tacaaagcat
     4021 acagatattc tctggatact ttagaatcac atgataaagg tattcttaaa ctgggtacaa
     4081 aaaactaagc cctcctaaaa aaggacatct aattatttgt tcccgctatc aaattgacag
     4141 tttatttaag aataccttgc cgcatattta ttaactcctt ttaaatagtc acttaaataa
     4201 tagcacgtaa gagcatattt gtaaaggaat ctccaatttt ttatcaaaaa gagtacatga
     4261 ttacaaagta tctgtaatca tgtaccaata tttgttattt tacaatcttc caattatctt
     4321 gtttttctaa tatcaactca aattgcgaga tttgggttgc ctttgtttct tgatctaaat
     4381 atttcacagc gacattcacg attacttggt tatcttttct ggtaaagata ggattcacca
     4441 attcctcgaa aacatattcc ttgttaatca taggtaacac atgattactt acatagtaag
     4501 tcaattcttt ttctgtggcg gtaggataga gctggaagaa cgtttctaaa aagctagtga
     4561 tttcgtccat cgtttctgta tctactgtat ggtcactttc tagctgtttg ggttggtagt
     4621 ccgatttttg aggtttctta ctcattgtcg gatttttgat aataaccata ttatctgatt
     4681 catctatatg gacaaccaca tgaaagctag acgatatagt ttccttgtct ttgtcttcgg
     4741 taataacttg ttcaacagaa aacagtacct caaaggtgtt ctcattcact tgagaaacct
     4801 gccacacctg tatatcgttc acactagaac tagtaggaat atccttacga atcatttctt
     4861 catttaatac ctgcaattct tccgtcaaat aatgggtcaa tttttcattt cgtttatcaa
     4921 tggcttcttg ggattgttgc cacgaaaagt agtctttcgc aaaggatttc acaaaacttt
     4981 ctacttggtt ggtatcgaca atctgctgtt taatgatttc tgtttcacgg acagtatgag
     5041 tgtcaatggc agtaaaattt ttatagattc caaatccgac actaccaatc aataaaaccc
     5101 ataaaacaaa ggttaatttc ttatgcgtgc caactttaat catcggaact tttctttgct
     5161 tgctcggttt tttagatttc tttggtttct ccttcttttc ttttcgctcg atttttataa
     5221 tcatttgatt cattcctttc ttagttatga ataacacgtc tagccccaat cagatggtct
     5281 tgccaataac gactgcttaa gtcgccataa ccgattgggt cgcctgcatg ataaaagcga
     5341 tttccctcta aatatatcgc cacatgagtc acatatgtgc ctgcattata ggttgaatga
     5401 aagaagataa ggtcgcctgc ttgggcttct tccatagaga tttcttgcgt agcgtcatac
     5461 tgatcttggg cgacacgagg gagagaaatg ccagctttgt catacaccca ttgtattaag
     5521 ccactacaat caaaagaggt ggtaggcgac gcccctccaa agacataggg aaacccttcg
     5581 tatttcaagg cttcgtccat aatgacttgc acggtttcat catcaaattc tgttggggaa
     5641 gtgtcggtta ggtattgtga aaccaactga acataaaact gatttccata gttatatcgc
     5701 caaccaccat tcacaggtat ggctatagga ttgggatagt ctgctttttg accacccgac
     5761 ttctctttag aaaactgttc ggctaattca taggtatatt ttttcccatg actcctcaca
     5821 taatttaaaa agccaccgcc ataattataa gattggataa cagaatctat atccactcct
     5881 tgttgttctg cactggttaa taattcactg aaatatttaa ccccttgttt gatggactct
     5941 tcggtactca aactgttggg cggtaaaccc aaagattcgg aactttgcat aacatcttct
     6001 gctgtaccgc ccgattccac ttgcataatg gctaagatat aagaaacata gtcttcaatc
     6061 ccatattcct taccatattt ttcaatcaac ggacggtgtg ccaacacttc tggtgaaacg
     6121 gtcacaccgc cttgtggaat agaagaatcc ttgttgtttt cgccactgtc cgtatcatct
     6181 gcaaagaaaa tgactaggaa taataagaga aaaaagaaga gagagaagac cacactacaa
     6241 atcactgtta tttttagctt catgtttgtt gtcctcgctt atccgttctt ttatttagct
     6301 tcctctgatt gaattgcttc ggtggtgtct tttgtttttg ctcttttttc aatggtcgct
     6361 gatgattttc acgtttagtg ttttgtgaca aaacaggcgt gacggtttgc tgacgcttgc
     6421 gaacatgctg ctggttattc tgaacaggag atgatgggtt ttcttttcgt cttttttctt
     6481 ttgctttatc cagctccaaa cgcttttgag caatgttctt tcgtttttgc tcctgctggt
     6541 tcatacgctg ttgtttgcgt tgagctttgg tatctgccat agattgttta aagtctgtta
     6601 cattatgagt gacattctct tttccttgat gaagggcata tttgacatgg gttggtgtcg
     6661 cctgtatctg ttgttttgcc tgctttcttt tattgactag acgatttttc gtgtccaata
     6721 catgggctgt cttttcacct aatcgttgcc cgataccgcc ttgttgcttt ttcacattgg
     6781 aaagtgctgt ttctttcttt ggtgtagtcc gttcattttg acgagtatgg ttagctttat
     6841 tggtttgatt ctgctttgtt gtcattgtct tttgcttcgt tgaccctgct tttccacgaa
     6901 aaatcttact catactgcgt tgaaatcttc tggtttgtcg attcatcatt tgacgaggtc
     6961 tgcgaaacac ttgtctgccc atgttttgag agtcgttgct ttgtaaagtg aataggctca
     7021 ttaaatcgcc cagtttaaaa taaatccctg caaaggtcac aatttgtaaa aacataatca
     7081 tgaaaaaggg agaacttgct gacagcgaat aaagcatagt ggaaatacta aaggcaacgg
     7141 taataatcaa ggtaatacca gcccttgtca taatcacatt aaataacttg gtaatggcat
     7201 gcctgcccat gccatcaaaa ctcggcaaca tacttaatag gaaactgatt ggcaaaaaca
     7261 tagcgaaaat aataaatagc acttgcgaaa aaatcatgat tcctgttaga aggaaaacaa
     7321 aaatcgagat accaatattg aatacaaaca aaaagaagac tgttcctaat ctggaaatcg
     7381 tcttcggtag ggttaaatag gtattgtcat gatcttcaat ttcttcaatc actacatttt
     7441 ctctatcttc accattgttt gtatctggac tggtagacaa taaactttcc acacgttctt
     7501 cccctaacgc ttctacatca gtggagccat attgaagcaa tagccatggt tgctttactt
     7561 gaacagagaa taagctgtca cgtatcatgt ccacactgtc tttcccttga ctatccgtgt
     7621 taggaagaac aatttttgtc ccaatattca gactggcggt actgacatct ttggaaaaat
     7681 cattgatttt cgcaatataa tcgggtgcat acgcaataaa ggaagccgat agaataaaca
     7741 ccacgacaaa gtttaaaaca gctcgtatca ctttcgtcgt ttctcgctta accaatcctg
     7801 tataggcaac atagattcca acaatgagaa taaaaagaag gaggaaaccg acataaaacc
     7861 ctgaactgga aaagccattc ggtgtaatgc ctgcaatcgt ctgcatgttt tcgccaattg
     7921 cttgggatgt ttgactaatg aaatctaagg cataggcttc tttcaccaaa taacctgtgg
     7981 cgttcgatag ataaagagaa attgtccaaa taaaattagt aatggcatac aatccataca
     8041 tcaccgattg tcctatgcca tcactccaat tccatggaag ccagccccag ctattgtcca
     8101 cataataatc aagctgatat tgattcaaac catacttgga gtattcattg cttgtatcaa
     8161 tggtttcatc aactaagcct gtcgcttgta ctgcattgcc cagcgtgcct aataaaagta
     8221 aaataacgcc cacacaaagg agcgttatcc aactatagtg aagaaatttc ttttgttttt
     8281 gtgtcattct tccacctcat ttcgcacagg cggtctggta tcaaaggcat gaagcaactc
     8341 ttcaaaaatc gggtgaaact gcataacccc tacacgacca tagatgtctg aaactaaaca
     8401 ttgtccattt tctaattcac gtaagcgttt ctgattgttt tcatcttcct tgtccacacc
     8461 aaaaaattct aaggtctttt tgatttccac taggtcagtg gaacgaaagg caaatttcaa
     8521 gccaatatta ttcttcatct tttcatctag taaatcgtcc gcgttttgag taacgaaata
     8581 gacccctgca ttcatggcac gtcctgctcg aaccaatcgc atagatagag cttttccttg
     8641 tgccacttgt aaaaaactcc aagcctcatc aaggtcaaca attttaaaaa tggaacggtc
     8701 agagtggata aagtctaaag caaaggtact aatgacaatc agcattgcca cacttaataa
     8761 ttccatggta gtgtattctt caaaagagct attggaatcg ggtaagacca aatctgccac
     8821 ttgaataata ttgagttgtt tttccaagct aatggattgg gtgacactgc catcactgaa
     8881 taacagatgg gcaaaatcat aatcgacaaa gctttcgata tggtcagcaa tactattgct
     8941 gatgggcgta tcttcttttc gcaattcatc aatcaccaat aacaaccctc gttgtttgct
     9001 ttgagttaca ctccgaatag ctttacgcag gacaggaaac ttctcgctat ctcggctgga
     9061 aatacctgtt aagaacgtga gaatatcaat ggcaaggctt tctgaatctt tcgggagttt
     9121 taaaatcaca taagggtcaa gtaacccttt attgtcgttc tcactcgtga gattaacaat
     9181 attgatttca tgggcaatat caggtaaggt ttctttccat tcaccacgtt cagcttttgg
     9241 gtcaacaatg gttgcctgtc cgccaaagag tactgaataa tagaccagca tattgttaga
     9301 gaatgatttt ccgccaccta atgaaccaat aaaagcggac gctaatgcat ttgttacaga
     9361 gcctttaatt ccttgacttg caaggctagg ttggagataa atatttctgc ctgtatcgag
     9421 attgtaaccg acgtaaatac cagaattttc accaatcatt tgtgtagcac cgaaaccaag
     9481 accagccaaa aaatccgacg tgacatattg aatatagtca ttgatatatc gcttgcttga
     9541 gggaataaat tcattatgca agcctagcat atcgccaaaa ggtcgtacta atttgataga
     9601 cagatcatca taaaaatctt ttacttcatc acaacgttgt ttcagctcgt ccaaattctt
     9661 tgctgatacc cgtaccacat agcttagttt gtacatagat tctttggttt ggtctaaatt
     9721 actttctaat tcatcgacag attctaaagc gtccaccaca ttagtactgg tttcgttgtc
     9781 gctttcccat gcgtggctat ctaaatcttt cagttctttc tttttattgc gaacggtaga
     9841 taaggctttt ttattggcga caatttcaac attcatactg gtatcaacgg ggaaagtaaa
     9901 ctgctgttgc tggtaataaa aaatttcact attagggaaa tccaattcac caacaatact
     9961 attaattgta aaatacgcca cataagtagt ggtatcttcc tgttcaatcc gtaaatagcg
    10021 ttgattttct tcgattaaac agcgagtggg tttgagaata tcgtatttct ttaccaacac
    10081 ttttttatct tcgtatcgtt tcggtaaatg gtagtcgtag tcatcaaaag ggacacctgt
    10141 ttgcccgtaa agatgttcaa taatataacc aaaatcatct ttctccaatt tacgaatctg
    10201 aaagcgtctg gataatttac tttctaacaa ctgttccatc ttagaaaagc gttcgatttc
    10261 cgccataggc atagaaacaa aatcgcccat taatttgtga ttcacaccat gaaagaattc
    10321 cgcaaatgct ttttgaattt ctttcccaac attttttacg gatacttcct gttcattgag
    10381 gtagagttta aaaccaataa aaaagcgata gtctacttga ttgtcaccaa tcatagaaat
    10441 taacgcttcg gtctgttcat ctattttttc gtaagctatc tcttttaatc gtccagtgat
    10501 ttcttttttc gattgttcct gtgtggctcg aatacttgat tcggttgcta gttgcagagc
    10561 atgaatcttg ccatcacgat tttgggcaat caattgacag aaattatcat gaacttgaat
    10621 tttttcatcg gaacttaaaa aactgtaatt atagggaatt aattcataat aagcgaatac
    10681 ttcgccatca tgattaaata cgagattatc ttccatatat ttaatcggat atgccatcta
    10741 gttgttcact ccttacaatc gtaatcgatt cattcattgt ttcttttttt aatcgtattt
    10801 gtttgccagc gtaggttact ttagggcgta gagcataggt aatgatagac tttagaaatc
    10861 catagggttt ctttccgtca aaggtctttt ggcacatgaa cccagtgaga acaatgggaa
    10921 tccctaagta tttgagtagt gctccgtcaa tcagagaaag tggtggtaaa ctgccaaaga
    10981 gtatgacgac aaacaaagaa acgataaacc atgtcatttg cgtaaaggta attgggaaag
    11041 ggagttgtaa atcattaatc gcataaatca ctttttctac tgaccaaata ctggtgtaac
    11101 ttcttatttt tttcatgtct tcacgtcctt tccgtaaaaa tagaaaaaga acaatcaaaa
    11161 taggtgattg ctctttcaat gctttaacta ttcaatttta ttttttgtcc attttgcaat
    11221 aataccttca tcagtttctt catcatctgg attcattgaa tatctgaaac ttaaattggt
    11281 atctgaccct aatcgttcat ataagctggg tctatacaat ctcgttttat tgtcattaaa
    11341 aggacttcgt tttcccagct caccatttcc ctttaaagga tttcttctaa ctgaaaaaca
    11401 atattttttt ctgggatctt ctgaatcact ttgacttaat gagaaacaca tggcttcttt
    11461 ttggtcttca tttttcttct cactaacttc tgtcggaata aatcgagcaa gagtttggct
    11521 aaattgaaat aaaacatctc ccaagttact actgtattcg ataccaaaat attctcttat
    11581 tgttttcgtg tctgtatata attttaccga attagcatag acagctagca tatctgaaaa
    11641 atcgttttcc tttaaaaatt ctagttttaa cagagcatat tctggaactt tttcctcttt
    11701 ttcaaatagc ttaacaagta caatataatt ttgctgtttg taacgaaagt aaaaggaatc
    11761 tatcacccag cctttatctt tcatgtctag ttttaatagt ttaagatttt caaatacatt
    11821 taccataatt tatacctctc cgtgctattt attaatttaa ttataacacg gattgttaaa
    11881 cttactgaca atattcaaat accccatgag aggtaactag gtaattaccc tctatttcca
    11941 tatcacgtcc caaagattgg tagtcaatat aattttgtaa attggaaggg acttcaccaa
    12001 gctgtcctgt ttcttccacg taataacggg cgacatcttc catcgaatca caatcactat
    12061 aacaatgaat atcctctttg ttttccagta attcttccaa gctactaaac cacatgcctt
    12121 gaatttcttt gagttcatta taaataggtg taccttcgat ttcttgaatg gcttcacata
    12181 gacggttgat ttcactaatc ggcgtgtatt cgtccacatc aaaaggaagc tcaaaatcat
    12241 gtattgcata ttcttcgtag tcctcattta aaccgattct ttctgccatt tcttcctcat
    12301 caattggtgg agtaaaccat gctccgacta attcaccttc attataccgt cctaaatttg
    12361 ccacatagac tttcatttcc aattcttatc acatcctttt tataaaaata gtcataaaaa
    12421 aagtagcaat cagttaatga cgctacttat caaggtatcc gctaatataa tctgtaactg
    12481 tacttgaaat acctattatt agttttgctt ctctctttcc aataatattt gtattagggt
    12541 gtgcaagtga atgcctattt cttgcttcat taagagagct aatcattcca tttgatgacc
    12601 ttatcgttgt cttcactata tcagcaagtt cagtaggttg aatttctttt tcaattaatt
    12661 gttgtatcct actatataat ttagatagat tttcgtctct aggaactgta atttcatatt
    12721 tttttagtat ttcaattaaa tatccatgaa aagcagtgtg aactctgtca aaggcacgtt
    12781 gatattcacc attactcata aaagactcag cgtcttcaat agcttgtgaa accacatcac
    12841 taattttcaa ctgactaaag ttaattactt cgtaattctc tggaagtggt actaaaatag
    12901 tatccactaa tacattatca ccttgtttat catatatgga gcttgctgtc tgggtaatca
    12961 aatctttatg actttcaaaa aagtctaaat catcaaaagg aactcttatt ttaagatatg
    13021 tgcaaagcgt attccagccc tttttatatc ctttataata agtaaaatcg tcagtctcat
    13081 ctaaagttac c
//
LOCUS       DAHUWM010000466         8061 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.124, whole genome shotgun sequence.
ACCESSION   DAHUWM010000466 DAHUWM010000000
VERSION     DAHUWM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.124"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            128..1564
                     /locus_tag="NK788_003020"
     CDS             128..1564
                     /locus_tag="NK788_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCH6771281.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="NK788_003021"
     CDS             1753..4200
                     /locus_tag="NK788_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCH6771282.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="NK788_003022"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="NK788_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCH6771283.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="NK788_003023"
     CDS             complement(5690..6793)
                     /locus_tag="NK788_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCH6771284.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="NK788_003024"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="NK788_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCH6771285.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DAHUWM010000467         6268 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.155, whole genome shotgun sequence.
ACCESSION   DAHUWM010000467 DAHUWM010000000
VERSION     DAHUWM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.155"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            391..1287
                     /locus_tag="NK788_003025"
     CDS             391..1287
                     /locus_tag="NK788_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCH6771286.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1437..2378)
                     /locus_tag="NK788_003026"
     CDS             complement(1437..2378)
                     /locus_tag="NK788_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCH6771287.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2504..3625
                     /locus_tag="NK788_003027"
     CDS             2504..3625
                     /locus_tag="NK788_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCH6771288.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3883..5385
                     /gene="eat(A)"
                     /locus_tag="NK788_003028"
     CDS             3883..5385
                     /gene="eat(A)"
                     /locus_tag="NK788_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCH6771289.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5413..5979
                     /locus_tag="NK788_003029"
     CDS             5413..5979
                     /locus_tag="NK788_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCH6771290.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2066 a         1100 c         1287 g         1815 t
ORIGIN      
        1 gggccggcga tcccaatcgc ttcgccactt cctttaaata gacctgcacc gattgctcca
       61 cctaaagcta acatggtgat atgtctggag ctaagttgtt ttttcaattt tttatcttcc
      121 ataaatgacc tctttctttt aaattcacaa tcaaaattta tattaagaat aaatagcaat
      181 atttaacaat gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt
      241 aggcgaattt taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt
      301 tcatacaaat ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga
      361 aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca
      421 aaaaataaat cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa
      481 gatttactac agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg
      541 atcgtttttt tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc
      601 aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg
      661 aagcaggcac aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca
      721 aaagaacttg ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct
      781 agcgaaccaa agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca
      841 gaaagctata agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca
      901 catcgaggga atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg
      961 gaatcgcgcg ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca
     1021 tttgaatcca tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat
     1081 catttttatc gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg
     1141 gaatttctgc atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca
     1201 gaaaattggg caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat
     1261 gacctctcct ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa
     1321 acaaacaagc acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt
     1381 gggcttaaaa aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat
     1441 tctgcgccaa ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt
     1501 gtagcaatat catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca
     1561 gtcaaacgat ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg
     1621 tcaggatcat aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct
     1681 tcttttgctg cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct
     1741 ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt
     1801 ccatttacac aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt
     1861 aaatttgctt cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc
     1921 aagttctcta gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga
     1981 ccagctgtcc acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt
     2041 acatcttcat gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg
     2101 gattggatcg aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag
     2161 tccacgtgtt cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc
     2221 acttctttac cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca
     2281 ggccaacgat cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg
     2341 ctgcccattg ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa
     2401 tgagttccct tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa
     2461 atgaaaaaaa gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc
     2521 aattgattca tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg
     2581 ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac
     2641 tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct
     2701 cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc
     2761 agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac
     2821 tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga
     2881 agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt
     2941 actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac
     3001 taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc
     3061 tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca
     3121 aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca
     3181 gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg
     3241 cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt
     3301 cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga
     3361 agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc
     3421 aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg
     3481 actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga
     3541 agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag
     3601 atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt
     3661 caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct
     3721 gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt
     3781 ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag
     3841 ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata
     3901 aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta
     3961 aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact
     4021 ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa
     4081 tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat
     4141 gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat
     4201 cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta
     4261 ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat
     4321 ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc
     4381 atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc
     4441 gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa
     4501 cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta
     4561 aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag
     4621 aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct
     4681 gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc
     4741 agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc
     4801 caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat
     4861 ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt
     4921 cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag
     4981 gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat
     5041 gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca
     5101 tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag
     5161 cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct
     5221 gaactatata catgggatga accattgaat tatttggatg tcttcaatca agaacaatta
     5281 gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc
     5341 ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa
     5401 gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat
     5461 gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat
     5521 gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat
     5581 ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca
     5641 caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg
     5701 gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta
     5761 gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt
     5821 cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat
     5881 gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca
     5941 ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa
     6001 ataaaatatt tcaaggcact gcttttgctt agcagtgcct tttttaggtt atgacaattc
     6061 ttttgataat ataaactcaa cctttagaaa tgaaatttaa ttattttaat aaaagtattg
     6121 aaattttgtt tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag
     6181 agggataata tgtttggctt ttcaatattt atgaacgatt ctttgtcaga agagaagaaa
     6241 cagtatatcc gccaaatggc atccaatg
//
LOCUS       DAHUWM010000468         5971 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.162, whole genome shotgun sequence.
ACCESSION   DAHUWM010000468 DAHUWM010000000
VERSION     DAHUWM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.162"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            complement(40..1026)
                     /locus_tag="NK788_003030"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="NK788_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="NK788_003031"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="NK788_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCH6771291.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="NK788_003032"
     CDS             1851..3005
                     /locus_tag="NK788_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCH6771292.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="NK788_003033"
     CDS             3220..4188
                     /locus_tag="NK788_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCH6771293.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="NK788_003034"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="NK788_003034"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCH6771294.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="NK788_003035"
     CDS             5218..5826
                     /locus_tag="NK788_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCH6771295.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1806 a         1160 c         1341 g         1664 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaaggttctg
     5941 ttgcaaagtt ttaaataaag aataaaatcc c
//
LOCUS       DAHUWM010000469         4158 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.216, whole genome shotgun sequence.
ACCESSION   DAHUWM010000469 DAHUWM010000000
VERSION     DAHUWM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.216"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            108..1586
                     /gene="msr(C)"
                     /locus_tag="NK788_003036"
     CDS             108..1586
                     /gene="msr(C)"
                     /locus_tag="NK788_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCH6771296.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1633..2079
                     /locus_tag="NK788_003037"
     CDS             1633..2079
                     /locus_tag="NK788_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCH6771297.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2278..2793)
                     /locus_tag="NK788_003038"
     CDS             complement(2278..2793)
                     /locus_tag="NK788_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCH6771298.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2910..4112
                     /locus_tag="NK788_003039"
     CDS             2910..4112
                     /locus_tag="NK788_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCH6771299.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1237 a          706 c          905 g         1310 t
ORIGIN      
        1 cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa acagattttt ctgctctttt
       61 ttagtatcga taattgttgt tttacatgaa ataggagtgt aaaacatatg gaaaatttag
      121 cagtaaatat aacaaatcta caagtcagct ttggcaacca gctagaacta tctattgatt
      181 ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg agaaaatggt gtcggtaaat
      241 cgactttgct caaactaata gccggtgaac tttttcccga tcatgggaaa atccaaacag
      301 agatcacctt caactacctg cctcaattaa cctatctcgc tgaagcaaag gacctaaatt
      361 tggaattagc cagtcatttc cagttaagac tggaagaaac ttcagagcgg aaatggagcg
      421 gaggggaaga acgaaagatc gagttgatac gtcttctttc ttcttatgaa caagggatgc
      481 ttctagacga gccaacaacc catctagata gaaaaagtat tgatcgactg attgaagagc
      541 ttcgttatta ttatggtacg ctggtttttg ttagtcatga tcgctatttt ctagatgagc
      601 tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg agagttttcg gggaattata
      661 gtgcctatct cactcaaaag gaattggaga aaaagactca gctacgagaa gcagagtcga
      721 tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa
      781 agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat cagactggat cggttgtctt
      841 cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga
      901 gaagactcca aaaaatagga gaaacaacca agccgcagca gatgaaacaa atccgttttc
      961 cagtaccaaa atctcttgaa ctccacagcc gctatcccat catgggacaa aatgtccaat
     1021 tggaaagaag tgggagaaca ttactggtaa atggtgattt tcagttttct ttaggtaaaa
     1081 aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac cttattagaa catatccgca
     1141 aacaaggaga aggaatcctt ctctctccga aagtaagctt tcaagtatat cagcaaaagg
     1201 gttatcaaat gacatctgaa gaatccatca ttcgttttgt catgagacaa acagagtttt
     1261 cagaatcgct tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa actttgacga
     1321 aaccgttatg tacattaagt gggggagaag cgacccgtct gacgattgct ttgcttttta
     1381 ctaagccaag taatgtgttg ctgttagatg aaccgactaa ttttattgat atggcaacga
     1441 tcgaagcttt agagaagcta atgcaaatat atccgggaac gattttgttt acttctcatg
     1501 attcttactt tgtcgagcgt acggctgatg aagtttatga aataaaaggg cagaaaataa
     1561 aaaaagtact tacgagaaat ttttaaatgt gctacgatga ttttcgtgac aaatttaaag
     1621 gagtggtaca gattgcctac aaataaaaaa gaaggaataa tttttacaac aatgatgtgt
     1681 tttttgatgg tcttggggat gagcgcgtat aatttatggc tgcatcaaga tcttcattta
     1741 tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc
     1801 atcatcggtg tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc agaaagcaag
     1861 gtccaaatga tcttaactat ttcatgcttg atggttttag gaatggtgac atgtatgtct
     1921 ttattcggtg tgatcatgga acaaggaata cctgatcaat tattctctgc ttatatgaat
     1981 gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt
     2041 tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat ggaacactgt tatttgaaaa
     2101 aaggccttgg catgcttttt gtcatagtct cttgaatgca gttctagaaa aatagtgatc
     2161 cgaacgatag gttttggcct gtcgttcgga tcgctttttc ttttccaact gtattgctct
     2221 tttattgaag ataatttgct cttcaataga tatttctaga aaggttctgt ttatccctta
     2281 ctcaaaaaac tgctgcattt gttctcttac ttgctgacac cattggagtt cctgctgaca
     2341 aagcataatt ctgcgctgga tcacaacata acgtccaaat tcttgtttct tgtctagcga
     2401 gaggtcattc aatttttttt gatttttttg catgattttg ttcagctgtt gttctctagt
     2461 gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa aaagcataga tttttgctag
     2521 aaattcatct ttttgcgtag gagcgggagt ttcctctttg atccattcct ctacaagagc
     2581 acgtccttca tcagtcaagt ggtagatctt tttttgagag tgctcgtctt tgtccaaaac
     2641 ggtcacataa ccctgctggt ctaacttggc aagcgttgta tagatctgac tgtgatgggc
     2701 ttcccaaaaa attgtgatca gctgcttcaa ttcataccca gaacgtggtt tggataaaag
     2761 cacgcataat aatatatagg ctaaagtatt cataaaaatc ctacctctcg tcttattttc
     2821 tttattatat cacagtaaag tttccacgtt ttacatgtgc atattgacat gtaaaataaa
     2881 tcatgtatga tgctaataga gagaaaggga tggaaatatt ggagaatcaa atagatatac
     2941 aagaacaaac aaatgaagaa acaaagggga aaagttcagc gctagttgta gcagctacct
     3001 gtatgatcgc ttttatggga gtgggtctag tagacccaat cttaaaaacg attgctgcac
     3061 agttaaacgc tacgccagct gaaacgaccc tgttatttac tagttacatg ttagtcactg
     3121 gtgtggtgat gctatttaca ggtttcttat ctagtcggat cggtttgaaa aaaacattga
     3181 tgacaggatt gtttatcatc gttgtattcg ctggtcttgg cggattatct gggaatatcg
     3241 gaatgctgat tgggttacgt gccggctggg gtgtcgggaa tgctttattt gtctctactg
     3301 ctttagcaac gatcgtcagt attttagttg gtaacacaga aaaagcaatc atgatgtatg
     3361 aagcagcact tggctgcgga atggctgttg gccctctagt cggtggggtc ttgggcagtg
     3421 gttcatggcg ttatccattt tttggggtag catttttgat gttttgcggt tttatagccc
     3481 tagcggtact tttacctgaa actgagaaac caaagagaaa agtaggcctt tgggaaggtg
     3541 tcaaagcgtt aagcaatcat aaacttcgtt cagttggact aatcgcattg ttatataatt
     3601 tcggattctt tactttactt gcgtatgcgc catttttatt gattggctat tctgaattag
     3661 aagtcggttt tgtatttttt ggctggggtg tgcttctggc tattgcttcg attttcgttg
     3721 caccggctct tgagcggaag tttacgactt atcggacaat tttagcagca tttgtcttat
     3781 tcattatttg tttgctgtta ttaggagctg gaacttctat gccagtcctt gtaccgatca
     3841 gcattgtgtt agcaggattt ttccaaggaa ttatcaatac actgttgacg acgattgcta
     3901 tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt gtccgctttt
     3961 tcggtggagc tttagcacca tttattgcag gtgtgattgg tgaacatatt ggtgaaagct
     4021 atgcgtttta ttttgcaggt gccattgttc ttatcagtgt atgttgtatc atttatcacc
     4081 gtaattattt tgctacaaat gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc
     4141 tgcatcaagt cataagaa
//
LOCUS       DAHUWM010000470         2957 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.254, whole genome shotgun sequence.
ACCESSION   DAHUWM010000470 DAHUWM010000000
VERSION     DAHUWM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.254"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..465
                     /locus_tag="NK788_003040"
     CDS             <1..465
                     /locus_tag="NK788_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCH6771300.1"
                     /translation="NNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLV
                     TNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHF
                     CTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            472..849
                     /locus_tag="NK788_003041"
     CDS             472..849
                     /locus_tag="NK788_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCH6771301.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1003..1785
                     /locus_tag="NK788_003042"
     CDS             1003..1785
                     /locus_tag="NK788_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCH6771302.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1911..2642)
                     /gene="erm(A)"
                     /locus_tag="NK788_003043"
     CDS             complement(1911..2642)
                     /gene="erm(A)"
                     /locus_tag="NK788_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCH6771303.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2700..2759)
                     /locus_tag="NK788_003044"
                     /pseudo
     CDS             complement(2700..2759)
                     /locus_tag="NK788_003044"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
BASE COUNT         1025 a          443 c          542 g          947 t
ORIGIN      
        1 aacaacggga tgccccagca tattgtgcag aaatttttgg ggcatgaaag cccagaaatg
       61 acaagcagat acgctcatat ctttgatgaa actctaaaaa atgaatttac taaatttcag
      121 gaaaaactgg ttaccaataa tggagatgtg cttgatctag atgaagataa tgaagtcgat
      181 gatgtagagc ttcaatggtt caagaaaaat ataaatgcac aagtgcttcc aaatggttat
      241 tgtagattgc cagtagtagc aggtggttgt ccacatgcga atgcatgctt agattgcact
      301 cacttctgta ccagtaagca attcttacca cagcacgaag aacagttaga gcgtacagaa
      361 gagttattag ccatagctaa ggataaacaa tggcaaagac aagtagagac taatagccgt
      421 gttaaagagc gtttagaaca aatcattgga agtttgacgg ggtaattatc aatggataaa
      481 caagttagaa atacaacaga aattgtacgt ttggcgaagc agaaatcaaa aaagacaagg
      541 gaaaaagtag acaaagcgat ttctaaattt tcgattgaag gtaaagttat taattttaat
      601 tcaatagcaa aggaagctaa tgtttctaaa tcatggcttt ataaggaaca cgatattagg
      661 caaagaatcg aatcccttcg tgagcgtcaa ataacagcaa atgtagtctc aaaacccaag
      721 aaaagttctc gttcggagga aatccttatt aaaaccttaa aaagaagagt aatggaatta
      781 gaaaaagaaa ataaaaaatt acagaaccaa attcaaaaat tatatggaga tctgtataat
      841 aaagaataat tattaatctg tagacaaatt gtgaaaggat gtacttaaac gctaacggtc
      901 agctttattg aacagtcatt taggtatatg tccaatctag ggtaagtaaa ttgagtatca
      961 atataaactt tatatgaaca taatcagtta cgaggtgaaa tcatgagcaa tttgattaac
     1021 ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt
     1081 tcaatagttg gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc
     1141 gatgtagatg ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta
     1201 acagaaagac taatgactat atcaggaaag attggaaata cggattctgt tagaccactt
     1261 gaagttacgg ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa
     1321 tttatatacg gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc
     1381 tatgatcctg atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt
     1441 ggtcctgatt cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt
     1501 aaggattctt tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta
     1561 accctagctc gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct
     1621 gcagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga
     1681 aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca
     1741 ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca
     1801 ataacaggtg cttactttta aaactactga tttattgata aatattgaac aatttttggg
     1861 aagaataaag cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac
     1921 aatttgtaac tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg
     1981 acatttgcat gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca
     2041 cggtttaccc acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat
     2101 ggttgatgtc gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat
     2161 agtggtggta cttttttgag catttttata tccatctcca ccattaatag taaacccaaa
     2221 gctcgttgca gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat
     2281 ttagcctgac tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata
     2341 ttaccatata tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga
     2401 atcactttta tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca
     2461 tcaatttcta tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct
     2521 tttcctgatc cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt
     2581 tcttttacat gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc
     2641 attataacct tctccttact ggttataatg aactgacatt aagcgttcat tataaccaat
     2701 taattttgat ttggttgata acgaacttta ttaataacaa atatagaaaa agtacccata
     2761 cttttatctc ctttccattt ttttcattat gaatgagata aagtaatttc tactactgcg
     2821 atactggtgc acataattga aacctcctta tgtaactgaa tatgattgta tcatcttttc
     2881 attttagaat aaacctttat atgtaatttt gcacttgaca cttcagatgg acgttttctt
     2941 caaaataata tgttttg
//
LOCUS       DAHUWM010000471         1504 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.338, whole genome shotgun sequence.
ACCESSION   DAHUWM010000471 DAHUWM010000000
VERSION     DAHUWM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.338"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            262..345
                     /locus_tag="NK788_003045"
                     /pseudo
     CDS             262..345
                     /locus_tag="NK788_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="NK788_003046"
     CDS             394..477
                     /locus_tag="NK788_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCH6771304.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="NK788_003047"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="NK788_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCH6771305.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAHUWM010000472         1350 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.358, whole genome shotgun sequence.
ACCESSION   DAHUWM010000472 DAHUWM010000000
VERSION     DAHUWM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.358"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            47..298
                     /locus_tag="NK788_003048"
                     /pseudo
     CDS             47..298
                     /locus_tag="NK788_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="NK788_003049"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="NK788_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCH6771306.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRAIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          323 g          368 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccgggc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAHUWM010000473         1350 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.359, whole genome shotgun sequence.
ACCESSION   DAHUWM010000473 DAHUWM010000000
VERSION     DAHUWM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.359"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            47..298
                     /locus_tag="NK788_003050"
                     /pseudo
     CDS             47..298
                     /locus_tag="NK788_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="NK788_003051"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="NK788_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCH6771307.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAHUWM010000474         1255 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.365, whole genome shotgun sequence.
ACCESSION   DAHUWM010000474 DAHUWM010000000
VERSION     DAHUWM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.365"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            1..1233
                     /gene="aph(2'')-I"
                     /locus_tag="NK788_003052"
                     /pseudo
     CDS             1..1233
                     /gene="aph(2'')-I"
                     /locus_tag="NK788_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          520 a           98 c          216 g          421 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttattgat gataatatgg ttttt
//
LOCUS       DAHUWM010000475         1225 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.369, whole genome shotgun sequence.
ACCESSION   DAHUWM010000475 DAHUWM010000000
VERSION     DAHUWM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.369"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            235..>1225
                     /locus_tag="NK788_003053"
     CDS             235..>1225
                     /locus_tag="NK788_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCH6771308.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRR"
BASE COUNT          404 a          195 c          235 g          391 t
ORIGIN      
        1 caaaatcaca aattttcatt caattatcat tttcaattgc attttataaa tcattttgta
       61 tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta cattttgtca
      121 ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag tggagtacta
      181 catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat
      241 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
      301 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
      361 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
      421 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
      481 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
      541 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
      601 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
      661 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
      721 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat
      781 gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg
      841 gattgtaaaa tctgttttaa gtacggttta tactatagcc ctgttagtac tccagcagat
      901 tttcggatgc ttactcctat tgtactggag caagccttta gaaaagctgg aacagaattg
      961 ttagagccat atcttagttt taaagtttat gcaccacagg aatatctttc acgggcatat
     1021 aacgatgctc ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc
     1081 attattattg gagaaattcc tgctcgatgt attcaagatt atcgcaatga tttaactttt
     1141 tttacaaatg ggcttagtgt ttgtttagca gagctaaaag gatatcaggt taccactggc
     1201 gaacctgttt gccagacccg tcgtc
//
LOCUS       DAHUWM010000476         1195 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.374, whole genome shotgun sequence.
ACCESSION   DAHUWM010000476 DAHUWM010000000
VERSION     DAHUWM010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.374"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            260..>1195
                     /gene="vanY"
                     /locus_tag="NK788_003054"
     CDS             260..>1195
                     /gene="vanY"
                     /locus_tag="NK788_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCH6771309.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          174 c          238 g          357 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt ggttctgttg caaagtttta aatctactat caaat
//
LOCUS       DAHUWM010000477         1146 bp    DNA     linear   BCT 01-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M08921
            SAMN28668636-rid14569193.guided.380, whole genome shotgun sequence.
ACCESSION   DAHUWM010000477 DAHUWM010000000
VERSION     DAHUWM010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28668636
            Sequence Read Archive: SRR19561652
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-JUN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001324133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001324133.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/08/2022 08:01:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 22 of 70
            Pseudo Genes (incomplete)         :: 38 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 5 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28668636-rid14569193.guided.380"
                     /isolate="M08921"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2020-10-01"
     gene            <1..349
                     /locus_tag="NK788_003055"
     CDS             <1..349
                     /locus_tag="NK788_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCH6771310.1"
                     /translation="NLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKTMFYLHS
                     IGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGKNDYKD
                     EIVYVYDYEKGDK"
     gene            350..>1146
                     /gene="aac(6')-Ie"
                     /locus_tag="NK788_003056"
     CDS             350..>1146
                     /gene="aac(6')-Ie"
                     /locus_tag="NK788_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCH6771311.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYI"
BASE COUNT          477 a           92 c          192 g          385 t
ORIGIN      
        1 taatttaaaa ggatttctta atgataccag ttcatttggg tttatagcta aagaaaataa
       61 taaaattata ggatttgcat attgctatac acttttaaga cctgatggaa aaacaatgtt
      121 ttatttacac tcaataggaa tgttacctaa ctatcaagac aaaggttatg gttcaaaatt
      181 attatctttt attaaggaat attctaaaga gattggttgt tctgaaatgt ttttaataac
      241 tgataaaggt aatcctagag cttgccatgt atatgaaaaa ttaggtggta aaaatgatta
      301 taaagatgaa atagtatatg tatatgatta tgaaaaaggt gataaataaa tgaatatagt
      361 tgaaaatgaa atatgtataa gaactttaat agatgatgat tttcctttga tgttaaaatg
      421 gttaactgat gaaagagtat tagaatttta tgatggtaga gataaaaaat atacattaga
      481 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
      541 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      601 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      661 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      721 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      781 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      841 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      901 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      961 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
     1021 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
     1081 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
     1141 tattag
//
